T1T2_yCalm.gb

LOCUS       T1T2_yCalm               300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Calmodulin binding peptide (calm) N-terminal purification tag from
            the E. coli Protein Expression Toolkit, defined as a T1T2 part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society. Based on a part in the OpenBioeconomy Lab's 
            E. coli Protein Expression Toolkit.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             21..158
                     /label="T1T2_yCalm"
                     /gene="T1T2_yCalm"
                     /translation="GVMPREETDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQL
                     A"
     misc_feature    161..164
                     /label="T2"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(166..171)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    172..177
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcCCATg ggtgttatgc caagagaaga aaccgattcc aagactgctt
       61 ctccatggaa gtctgctaga ttgatggttc atactgtcgc taccttcaac tccattaagg
      121 aactgaacga acgttggaga tctttgcaac aattggctgg GTCAcGAGAC CACTAGTatc
      181 gttgtttcgg gaggttgcgg gttctcgtgg tcgtaggttg aggcgtgtac tcgtctggaa
      241 accagcgatc gtgcgaccta ttagtgcgat gacctgtttt gctccgtcac agctaggctc
//