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OYC-dropout-mScarlet.gb
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OYC-dropout-mScarlet.gb
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LOCUS OYC-dropout-mScarlet 853 bp DNA linear UNC 03-JUL-2023
DEFINITION Open Yeast mScarlet dropout with J23100 promoter and strong pET RBS. Used
as a receiving vector for OYC GGA. Prefix: AAAA Suffix: CGAA
ACCESSION .
VERSION .
KEYWORDS Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 886)
AUTHORS Scott Pownall, Open Science Network Society
TITLE Direct Submission
JOURNAL https://iGEM.org/
REFERENCE 2 (bases 50 to 120 and 826 to 870)
AUTHORS Marcos Valenzuela-Ortega and Christopher French
TITLE Joint universal modular plasmids (JUMP): a flexible vector platform for
synthetic biology.
JOURNAL Synthetic Biology, 2021, 6(1): ysab003
PubMed ID: 31148366
doi: 10.1093/synbio/ysab003
REFERENCE 3 (bases 121 to 825)
AUTHORS Matthew C Haines, Benedict Carling, James Marshall, Vasily A Shenshin,
Geoff S Baldwin, Paul Freemont, Marko Storch
TITLE basicsynbio and the BASIC SEVA collection: software and vectors for an
established DNA assembly method.
JOURNAL Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023.
PubMed ID: 36381610
doi: 10.1093/synbio/ysac023
COMMENT Promoter & RBS from pJUMP vectors; PacI/SpeI for cloning into SEVA; BbsI
for cloning into pOpen-v3; mScarlet sequence from BASIC_SEVA; Can replace
either BsaI site with overlapping oligos for low cost customization.
FEATURES Location/Qualifiers
protein_bind 1..8
/label="PacI"
/ApEinfo_fwdcolor="#00FF2C"
/ApEinfo_revcolor="#00FF2C"
protein_bind 10..15
/label="XbaI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
misc_feature 17..20
/label="S1"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
protein_bind complement(22..27)
/label="BsaI"
/ApEinfo_fwdcolor="#7FFFD4"
/ApEinfo_revcolor="#7FFFD4"
protein_bind 28..33
/label="XhoI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
promoter 38..72
/label="J23100 promoter"
/ApEinfo_fwdcolor="#006400"
/ApEinfo_revcolor="#006400"
RBS 77..97
/label="pET RBS"
/note="efficient ribosome binding site from pJUMP & pET vectors"
/ApEinfo_fwdcolor="#006400"
/ApEinfo_revcolor="#006400"
CDS 105..809
/label="mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYKGS"
protein_bind 831..836
/label="MfeI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
protein_bind 837..842
/label="BsaI"
/ApEinfo_fwdcolor="#7FFFD4"
/ApEinfo_revcolor="#7FFFD4"
misc_feature 844..847
/label="S5"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
protein_bind 848..853
/label="SpeI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
ORIGIN
1 TTAATTAAcT CTAGAcAAAA tGAGACCctc gagggagTTG ACGGCTAGCT CAGTCCTAGG
61 TACAGTGCTA GCgtacTTGT TTAACTTTAA GAAGGAGata tacaATGGTT AGCAAAGGCG
121 AGGCGGTTAT CAAGGAGTTT ATGCGTTTTA AGGTTCACAT GGAGGGTAGC ATGAATGGTC
181 ACGAGTTCGA GATCGAGGGT GAAGGCGAGG GTCGTCCGTA CGAAGGCACC CAGACCGCGA
241 AGCTGAAAGT GACCAAGGGT GGCCCGCTGC CGTTCAGCTG GGACATCCTG AGCCCGCAGT
301 TCATGTATGG CAGCCGTGCG TTTACCAAAC ACCCGGCGGA CATTCCGGAT TACTATAAGC
361 AAAGCTTCCC GGAAGGTTTT AAATGGGAGC GTGTTATGAA CTTCGAAGAT GGTGGCGCGG
421 TGACCGTTAC CCAGGACACC AGCCTGGAGG ATGGCACCCT GATTTACAAG GTGAAACTGC
481 GTGGCACCAA CTTTCCGCCG GATGGTCCGG TTATGCAGAA GAAAACGATG GGTTGGGAAG
541 CGAGCACCGA GCGTCTGTAT CCGGAAGATG GCGTGCTGAA GGGTGATATC AAAATGGCGC
601 TGCGTCTGAA GGACGGTGGC CGTTACCTGG CGGATTTTAA GACCACCTAT AAAGCGAAGA
661 AACCGGTGCA AATGCCGGGT GCGTACAACG TTGACCGTAA ACTGGATATT ACCAGCCACA
721 ACGAGGATTA TACCGTGGTT GAGCAATATG AGCGTAGCGA GGGTCGCCAC AGCACCGGCG
781 GCATGGACGA ACTGTATAAG GGATCCTGAc ttctgactga gttgcacgct caattgGGTC
841 TCtCGAAact agt
//