CE_kp_kar2p.gb

LOCUS       CE_kp_kar2p             2070 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Komagataella phaffi (Pichia pastoris) kar2p accessory protein for
            improving heterologous protein expression., defined as a CD part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             17..2050
                     /label="CE_kp_kar2p"
                     /gene="CE_kp_kar2p"
                     /translation="MLSLKPSWLTLAALMYAMLLVVVPFAKPVRADDVESYGTVIGIDL
                     GTTYSCVGVMKSGRVEILANDQGNRITPSYVSFTEDERLVGDAAKNLAASNPKNTIFDI
                     KRLIGMKYDAPEVQRDLKRLPYTVKSKNGQPVVSVEYKGEEKSFTPEEISAMVLGKMKL
                     IAEDYLGKKVTHAVVTVPAYFNDAQRQATKDAGLIAGLTVLRIVNEPTAAALAYGLDKT
                     GEERQIIVYDLGGGTFDVSLLSIEGGAFEVLATAGDTHLGGEDFDYRVVRHFVKIFKKK
                     HNIDISNNDKALGKLKREVEKAKRTLSSQMTTRIEIDSFVDGIDFSEQLSRAKFEEINI
                     ELFKKTLKPVEQVLKDAGVKKSEIDDIVLVGGSTRIPKVQQLLEDYFDGKKASKGINPD
                     EAVAYGAAVQAGVLSGEEGVDDIVLLDVNPLTLGIETTGGVMTTLINRNTAIPTKKSQI
                     FSTAADNQPTVLIQVYEGERALAKDNNLLGKFELTGIPPAPRGTPQVEVTFVLDANGIL
                     KVSATDKGTGKSESITINNDRGRLSKEEVDRMVEEAEKYAAEDAALREKIEARNALENY
                     AHSLRNQVTDDSETGLGSKLDEDDKETLTDAIKDTLEFLEDNFDTATKEELDEQREKLS
                     KIAYPITSKLYGAPEGGTPPGGQGFDDDDGDFDYDYDYDHDEL"
     misc_feature    2054..2057
                     /label="E"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(2059..2064)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    2065..2070
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcAatgc tgtcgttaaa accatcttgg ctgactttgg cggcattaat
       61 gtatgccatg ctattggtcg tagtgccatt tgctaaacct gttagagctg acgatgtcga
      121 atcttatgga acagtgattg gtatcgattt gggtaccacg tactcttgtg tcggtgtgat
      181 gaagtcgggt cgtgtagaaa ttcttgctaa tgaccaaggt aacagaatca ctccttccta
      241 cgttagtttc actgaagatg agagactggt tggtgatgct gctaagaact tagctgcttc
      301 taacccaaaa aacaccatct ttgatattaa gagattgatc ggtatgaagt atgatgcccc
      361 agaggtccaa agagacttga agcgtttgcc ttacactgtc aagagcaaga acggccaacc
      421 tgtcgtttct gtcgagtaca agggtgagga gaagtctttc actcctgagg agatttccgc
      481 catggtcttg ggtaagatga agttgatcgc tgaggactac ttaggaaaga aagtcactca
      541 tgctgtcgtt accgttccag cctacttcaa cgacgctcaa cgtcaagcca ctaaggatgc
      601 cggtttgatc gccggtttga ctgttctgag aattgtgaac gagcctaccg ccgctgccct
      661 tgcttacggt ttggacaaga ctggtgagga aagacagatc atcgtctacg acttgggtgg
      721 aggaaccttc gatgtttctc tgctttctat tgagggtggt gctttcgagg ttcttgctac
      781 cgccggtgac acccacttgg gtggtgagga ctttgactac agagttgttc gccacttcgt
      841 taagattttc aagaagaagc ataacattga catcagcaac aatgataagg ctttaggtaa
      901 gctgaagaga gaggtcgaaa aggccaagcg tactttgtcc tctcagatga ctaccagaat
      961 tgagattgac tctttcgtcg acggtatcga cttctctgag caactgtcta gagctaagtt
     1021 tgaggagatc aacattgaat tattcaagaa aacactgaaa ccagttgaac aagtcctcaa
     1081 agacgctggt gtcaagaaat ctgaaattga tgacattgtc ttggttggtg gttctaccag
     1141 aattccaaag gttcaacaat tattggagga ttactttgac ggaaagaagg cttctaaggg
     1201 aattaaccca gatgaagctg tcgcatacgg tgctgctgtt caggctggtg ttttgtctgg
     1261 tgaggaaggt gtcgatgaca tcgtcttgct tgatgtgaac cccctaactc tgggtatcga
     1321 gactactggt ggcgttatga ctaccttaat caacagaaac actgctatcc caactaagaa
     1381 atctcaaatt ttctccactg ctgctgacaa ccagccaact gtgttgattc aagtttatga
     1441 gggtgagaga gccttggcta aggacaacaa cttgcttggt aaattcgagc tgactggtat
     1501 tccaccagct ccaagaggta ctcctcaagt tgaggttact tttgttttag acgctaacgg
     1561 aattttgaag gtttctgcca ccgataaggg aactggaaaa tccgagtcca tcaccatcaa
     1621 caatgatcgt ggtagattgt ccaaggagga ggttgaccgt atggttgaag aggccgagaa
     1681 gtacgccgct gaggatgctg cactaagaga aaagattgag gctagaaacg ctctggagaa
     1741 ctacgctcat tcccttagga accaagttac tgatgactct gaaaccgggc ttggttctaa
     1801 attggacgag gacgacaaag agacattgac agatgccatc aaagataccc tagagttctt
     1861 ggaagataac ttcgacaccg caaccaagga agaattagac gaacaaagag aaaagctttc
     1921 caagattgct tacccaatca cttctaagct atacggtgct ccagagggtg gtactccacc
     1981 tggtggtcaa ggttttgacg atgatgatgg agactttgac tacgactatg actatgatca
     2041 tgatgagttg taaGCTTcGA GACCACTAGT
//