CE_kp_RPP0.gb

LOCUS       CE_kp_RPP0               972 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Komagataella phaffi (Pichia pastoris) RPP0 accessory protein for
            improving heterologous protein expression., defined as a CE part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             17..952
                     /label="CE_kp_RPP0"
                     /gene="CE_kp_RPP0"
                     /translation="MGGINEKKAEYFNKLRELLESYKSIFIVGVDNVSSQQMHEVRQTL
                     RGKAVILMGKNTMVRKALRDFVEELPVFEKLLPFVRGNIGFVFTNEDLKTIRDVIIENR
                     VAAPARPGAIAPLDVFIPAGNTGMEPGKTSFFQALGVPTKISRGTIEITSDVKVVEKDS
                     RVGPSEAQLLNMLNISPFTYGLTVVQVFDDGQVFPANILDITDDELLSHFTSAISTIAQ
                     ISLAAGYPTLPSVGHSVVNHYKNVLAVSIATDYSFEGSEAIKDRLANPEAYAAAAPAAG
                     EASAGAEETAAAAEEEDEESEDDDMGFGLFD"
     misc_feature    956..959
                     /label="E"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(961..966)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    967..972
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcAatgg gaggtatcaa cgaaaagaaa gccgagtact ttaacaagct
       61 cagagagctt ttggaatctt acaagtccat ctttattgtc ggtgttgaca atgtctcctc
      121 tcagcaaatg cacgaggtca gacagactct ccgtggtaaa gctgtgattc ttatgggtaa
      181 gaacaccatg gtcagaaagg ccttgagaga cttcgtcgag gagctcccag tctttgagaa
      241 gctgttgcct ttcgtcagag gtaacattgg tttcgttttc accaacgagg acttgaaaac
      301 catcagagat gtgatcatcg agaacagagt cgctgcccca gccagaccag gtgctatcgc
      361 tcctttggat gttttcattc ctgccggtaa cactggtatg gaaccaggta agacctcttt
      421 cttccaagct cttggtgtcc caaccaagat ttccagaggt accattgaga tcaccagtga
      481 tgttaaggtt gttgagaaag actccagagt tggtccttcc gaggctcaat tgttgaacat
      541 gttgaacatc tctcctttca cctacggttt gactgttgtt caagttttcg acgatggaca
      601 agttttccca gctaacattt tggacatcac cgatgacgag ctgttgtctc acttcacctc
      661 tgccatctct accattgccc aaatctcttt ggctgctggt tacccaactt tgccatccgt
      721 tggtcactct gttgtcaacc actacaagaa tgttttggct gtctccattg ctactgacta
      781 ctcctttgag ggttctgaag ctatcaagga cagattggct aaccctgagg cttacgctgc
      841 tgctgctcct gctgccggtg aggcttctgc tggtgccgag gagactgctg ccgctgctga
      901 ggaggaggac gaagagtctg aggacgacga catgggattc ggtttgtttg attaaGCTTc
      961 GAGACCACTA GT
//