-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathCE_kp_PDI1.gb
81 lines (81 loc) · 4.54 KB
/
CE_kp_PDI1.gb
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
LOCUS CE_kp_PDI1 1587 bp DNA linear SYN 30-NOV-2023
DEFINITION Komagataella phaffi (Pichia pastoris) PDI1 accessory protein for
improving heterologous protein expression., defined as a CE part.
ACCESSION .
VERSION .
KEYWORDS Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE .
ORGANISM .
.
COMMENT Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
PacI and SpeI to permit transfer to the SEVA family of vectors.
Final sequences designed by Ian Cavén and Scott Pownall of Open
Science Network Society.
FEATURES Location/Qualifiers
protein_bind 1..8
/label="PacI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
protein_bind 9..14
/label="BsaI"
/ApEinfo_fwdcolor="#00FF2C"
/ApEinfo_revcolor="#00FF2C"
misc_feature 16..19
/label="C"
/ApEinfo_fwdcolor="#ffef86"
/ApEinfo_revcolor="#ffef86"
CDS 17..1567
/label="CE_kp_PDI1"
/gene="CE_kp_PDI1"
/translation="MQFNWNIKTVASILSALTLAQASDQEAIAPEDSHVVKLTEATFES
FITSNPHVLAEFFAPWCGHCKKLGPELVSAAEILKDNEQVKIAQIDCTEEKELCQGYEI
KGYPTLKVFHGEVEVPSDYQGQRQSQSIVSYMLKQSLPPVSEINATKDLDDTIAEAKEP
VIVQVLPEDASNLESNTTFYGVAGTLREKFTFVSTKSTDYAKKYTSDSTPAYLLVRPGE
EPSVYSGEELDETHLVHWIDIESKPLFGDIDGSTFKSYAEANIPLAYYFYENEEQRAAA
ADIIKPFAKEQRGKINFVGLDAVKFGKHAKNLNMDEEKLPLFVIHDLVSNKKFGVPQDQ
ELTNKDVTELIEKFIAGEAEPIVKSEPIPEIQEEKVFKLVGKAHDEVVFDESKDVLVKY
YAPWCGHCKRMAPAYEELATLYANDEDASSKVVIAKLDHTLNDVDNVDIQGYPTLILYP
AGDKSNPQLYDGSRDLESLAEFVKERGTHKVDALALRPVEEEKEAEEEAESEADAHDEL
"
misc_feature 1571..1574
/label="E"
/ApEinfo_fwdcolor="#ffef86"
/ApEinfo_revcolor="#ffef86"
protein_bind complement(1576..1581)
/label="BsaI"
/ApEinfo_fwdcolor="#00FF2C"
/ApEinfo_revcolor="#00FF2C"
protein_bind 1582..1587
/label="SpeI"
/ApEinfo_fwdcolor="#8A2BE2"
/ApEinfo_revcolor="#8A2BE2"
ORIGIN
1 TTAATTAAGG TCTCcAatgc agtttaattg gaacatcaag accgttgcct caatcctcag
61 tgcccttact cttgctcagg cttctgacca agaagcaatc gcacccgaag attcgcacgt
121 tgttaagctg acagaggcta ccttcgaaag ctttattact agcaacccac atgtactcgc
181 tgaattcttc gctccctggt gcggccattg caagaaactg ggtccagagt tggtgagcgc
241 cgctgaaatt ctaaaagata acgaacaagt aaagatcgcc cagatcgact gcaccgaaga
301 gaaagagttg tgccagggtt atgagataaa gggctaccca accctgaaag tctttcacgg
361 agaagtggaa gtgccttccg attaccaggg gcagcgtcaa tcacagtcga tagtatctta
421 catgttaaaa caatccctgc caccagtttc cgagattaac gccacgaagg acttggatga
481 tactattgct gaagctaagg aacctgttat cgttcaggtt ctgccagagg acgctagcaa
541 tctagagtcc aatacaactt tctatggtgt cgcaggaaca ctgcgtgaaa agtttacctt
601 cgtttcaacc aaatcaaccg actacgctaa gaagtatacc tcagattcaa caccggcata
661 cttattggtg aggccagggg aagaaccaag cgtctattca ggagaagaac tagacgaaac
721 ccacttagtt cattggatag acatcgaatc gaagccactt ttcggtgata tcgatggttc
781 tacatttaag tcctacgccg aggccaatat tccgttggct tattactttt acgaaaatga
841 ggagcagaga gcagcagccg ctgacatcat caagccattc gcaaaggaac agagaggtaa
901 gatcaatttc gtcggtctgg acgcagtcaa gtttggcaaa cacgcaaaga atctaaatat
961 ggacgaggaa aagcttccac tcttcgtaat ccacgacctt gtttccaata agaagttcgg
1021 tgtaccgcag gatcaggagc ttaccaataa ggacgttact gaattaatag aaaagtttat
1081 tgctggtgaa gctgagccca tcgtcaagtc tgaacctatc cccgagatac aagaggaaaa
1141 ggtatttaaa cttgtaggta aagctcatga cgaggtagta tttgacgagt caaaggacgt
1201 attggttaaa tattatgctc cctggtgcgg gcattgcaaa cgaatggccc ccgcctacga
1261 agagttggca accctgtatg caaacgacga ggacgcaagc tctaaagtcg tcatcgctaa
1321 gttggaccat acgctgaatg acgtggataa tgtagacatc cagggatacc caacactgat
1381 actgtacccc gcaggagaca agagtaaccc acagttatac gacgggtcaa gggatttgga
1441 gagcttagca gaattcgtta aagaacgagg tactcataag gttgacgcac tggctcttag
1501 gcctgttgaa gaggagaaag aggccgagga agaggcagag tcggaagccg atgcccatga
1561 tgaactctaa GCTTcGAGAC CACTAGT
//