BT1_preOst1_proαMF_signal.gb

LOCUS       BT1_preOst1_proαMF_signal 313 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  S. cerevisiae Ost1 signal sequence paired with the α-factor pro
            region defined as a BT1 part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..295
                     /label="BT1_preOst1_proαMF_signal"
                     /gene="BT1_preOst1_proαMF_signal"
                     /translation="MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYS
                     DLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     misc_feature    297..300
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(302..307)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    308..313
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgaggcaggt ttggttctct tggattgtgg gattgttcct
       61 atgttttttc aacgtttctt ctgctgctcc agtcaacact acaacagaag atgaaacggc
      121 acaaattccg gctgaagctg tcatcggtta ctcagattta gaaggggatt tcgatgttgc
      181 tgttttgcca ttttccaaca gcacaaataa cgggttattg tttataaata ctactattgc
      241 cagcattgct gctaaagaag aaggggtatc tctcgagaaa agagaggctg aagcttCCAT
      301 aGAGACCACT AGT
//