BT1_killer_protein_signal.gb

LOCUS       BT1_killer_protein_signal 300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Killer protein signal sequence from Saccharomyces cerevisiae fused
            to αMF_no_EAEA defined as a BT1 part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..268
                     /label="BT1_killer_protein_signal"
                     /gene="BT1_killer_protein_signal"
                     /translation="MTKPTQVLVRSVSILFFITLLHLVVAMRFPSIFTAVLFAASSALA
                     APVNTTTEDELEGDFDVAVLPFSASIAAKEEGVSLEKR"
     misc_feature    270..273
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(275..280)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    281..286
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    287..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgaccaaacc aacgcaagtc ttagttcgtt cagtctctat
       61 tttattcttc atcacactgt tgcacttggt tgttgcaatg agatttccta gtattttcac
      121 tgctgtgcta tttgccgcta gttccgctct agctgctcca gttaatacta ctactgaaga
      181 tgaattggag ggtgacttcg atgttgctgt tctgcctttt tccgcttcta tcgcagccaa
      241 ggaagaaggt gtatccctag agaagcgttC CATgGAGACC ACTAGTgcag cttggttctt
//