BT1_inulinase_signal.gb

LOCUS       BT1_inulinase_signal     300 bp    DNA     linear   SYN 28-NOV-2023
DEFINITION  Inulinase secretion signal defined as a BT1 part. 
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
REFERENCE   1  (bases 20 to 238)
  AUTHORS   Ulrike Obst, Timothy K Lu, Volker Sieber.
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Jun 16;6(6):1016-1025. PMID: 28252957 DOI:
            10.1021/acssynbio.6b00337
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by PacI 
            and SpeI to permit transfer to the SEVA family of vectors. Final sequences 
            designed by Ian Cavén and Scott Pownall of Open Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..238
                     /label="BT1_inulinase_signal"
                     /gene="BT1_inulinase_signal"
                     /translation="MKLAYSLLLPLAGVSAMRFPSIFTAVLFAASSALAAPVNTTTEDE
                     LEGDFDVAVLPFSASIAAKEEGVSLEKR"
     misc_feature    240..243
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(245..250)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    251..256
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    257..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgaaactggc ttactccctg ttgctacctc tggctggagt
       61 ttccgctatg agatttccta gtattttcac tgctgtgcta tttgccgcta gttccgctct
      121 agctgctcca gttaatacta ctactgaaga tgaattggag ggtgacttcg atgttgctgt
      181 tctgcctttt tccgcttcta tcgcagccaa ggaagaaggt gtatctctag agaagcgttC
      241 CATgGAGACC ACTGTCgaat ttcaatcttg tctggtgtgg aagccaacca ggcactcatc
//