BT1_glucoamylase_signal.gb

LOCUS       BT1_glucoamylase_signal  300 bp    DNA     linear   SYN 28-NOV-2023
DEFINITION  Glucoamylase secretion signal defined as a BT1 part. 
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
REFERENCE   1  (bases 20 to 244)
  AUTHORS   Ulrike Obst, Timothy K Lu, Volker Sieber.
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion
            Libraries.
  JOURNAL   ACS Synth Biol. 2017 Jun 16;6(6):1016-1025. PMID: 28252957 DOI:
            10.1021/acssynbio.6b00337
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by PacI 
            and SpeI to permit transfer to the SEVA family of vectors. Final sequences 
            designed by Ian Cavén and Scott Pownall of Open Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..244
                     /label="BT1_glucoamylase_signal"
                     /gene="BT1_glucoamylase_signal"
                     /translation="MSFRSLLALSGLVCSGLAMRFPSIFTAVLFAASSALAAPVNTTTE
                     DELEGDFDVAVLPFSASIAAKEEGVSLEKR"
     misc_feature    246..249
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(251..256)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    257..262
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    263..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgtctttcag atccctattg gcattgtcag ggttggtctg
       61 ttctggattg gctatgagat ttcctagtat tttcactgct gtgctatttg ccgctagttc
      121 cgctctagct gctccagtta atactactac tgaagatgaa ttggagggtg acttcgatgt
      181 tgctgttctg cctttttccg cttctatcgc agccaaggaa gaaggtgtat ccctagagaa
      241 gcgttCCATg GAGACCACTA GTgtttgcac ttctggagag agaatatgct cggtcgtatc
//