BT1_Sce_αMF_signal.gb

LOCUS       BT1_Sce_αMF_signal       300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Saccharomyces cereviseae alpha mating factor secretion signal
            defined as a BT1 part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..274
                     /label="BT1_Sce_αMF_signal"
                     /gene="BT1_Sce_αMF_signal"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     misc_feature    276..279
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(281..286)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    287..292
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgagatttcc ttcaattttt actgctgttt tattcgcagc
       61 atcctccgca ttagctgctc cagtcaacac tacaacagaa gatgaaacgg cacaaattcc
      121 ggctgaagct gtcatcggtt actcagattt agaaggggat ttcgatgttg ctgttttgcc
      181 attttccaac agcacaaata acgggttatt gtttataaat actactattg ccagcattgc
      241 tgctaaagaa gaaggggtat ctttagagaa aagatCCATa GAGACCACTA GTtaaccgca
//