BC_pre_Ost1_pro_αMF_I_His_TEVcut.gb

LOCUS       BC_pre_Ost1_pro_αMF_I_His_TEVcut 356 bp    DNA     linear   SYN 28-NOV-2023
DEFINITION  S. cerevisiae Ost1 signal sequence paired with the α-mating factor pro
            region with His purification tag and TEV cleavage linker. Defined as
            a BC part. 
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM
SOURCE      .
  ORGANISM  .
            .
REFERENCE   1  (bases 20 to 337)
  AUTHORS   Juan J Barrero, Jason C Casler, Francisco Valero, Pau Ferrer,
            Benjamin S Glick.
  TITLE     An improved secretion signal enhances the secretion of model
            proteins from Pichia pastoris.
  JOURNAL   Microb Cell Fact. 2018 Oct 12;17(1):161 PubMed ID: 30314480 doi:
            10.1186/s12934-018-1009-5.
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by 
            PacI and SpeI to permit transfer to the SEVA family of vectors. Final 
            sequences designed by Ian Cavén and Scott Pownall of Open Science Network 
            Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="B"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..337
                     /label="BC_pre_Ost1_pro_αMF_I_His_TEVcut"
                     /gene="BC_pre_Ost1_pro_αMF_I_His_TEVcut"
                     /translation="MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYS
                     DLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEAGHHHHHHGENLY
                     FQ"
     misc_feature    340..343
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(345..350)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    351..356
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcTACTa tgaggcaggt ttggttctct tggattgtgg gattgttcct
       61 atgttttttc aacgtttctt ctgctgctcc agtcaacact acaacagaag atgaaacggc
      121 acaaattccg gctgaagctg tcatcggtta ctcagattta gaaggggatt tcgatgttgc
      181 tgttttgcca ttttccaaca gcacaaataa cgggttattg tttataaata ctactattgc
      241 cagcattgct gctaaagaag aaggggtatc tctcgagaaa agagaggctg aagctggaca
      301 tcaccatcac catcacggag agaatctgta ctttcaaggA ATGgGAGACC ACTAGT
//