diff --git a/CODEOWNERS b/CODEOWNERS
index cdb12455397b..6033ffbf881f 100644
--- a/CODEOWNERS
+++ b/CODEOWNERS
@@ -607,6 +607,7 @@
 /scripts/print_docker_image.sh            @nrfconnect/ncs-ci
 /scripts/print_toolchain_checksum.sh      @nrfconnect/ncs-ci
 /scripts/sdp/                             @nrfconnect/ncs-ll-ursus
+/scripts/twister/alt/zephyr/tests/drivers/mspi/api/testcase.yaml @nrfconnect/ncs-ll-ursus
 
 /scripts/docker/*.rst                     @nrfconnect/ncs-doc-leads
 /scripts/hid_configurator/*.rst           @nrfconnect/ncs-si-bluebagel-doc
diff --git a/scripts/twister/alt/zephyr/tests/drivers/mspi/api/testcase.yaml b/scripts/twister/alt/zephyr/tests/drivers/mspi/api/testcase.yaml
new file mode 100644
index 000000000000..131c12ef39ee
--- /dev/null
+++ b/scripts/twister/alt/zephyr/tests/drivers/mspi/api/testcase.yaml
@@ -0,0 +1,30 @@
+tests:
+  drivers.mspi.api:
+    tags:
+      - drivers
+      - mspi
+      - api
+    filter: dt_compat_enabled("zephyr,mspi-emul-controller") or
+            dt_compat_enabled("ambiq,mspi-controller")
+    harness: ztest
+    platform_allow:
+      - native_sim
+      - apollo3p_evb
+    integration_platforms:
+      - native_sim
+
+  drivers.mspi.api.emspi:
+    tags:
+      - drivers
+      - mspi
+      - api
+    harness: ztest
+    platform_allow:
+      - nrf54l15dk_nrf54l15_cpuapp
+    integration_platforms:
+      - nrf54l15dk_nrf54l15_cpuapp
+    extra_args:
+      - SB_CONFIG_VPR_LAUNCHER=n
+      - SB_CONFIG_PARTITION_MANAGER=n
+      - SB_CONFIG_SDP=y
+      - SB_CONFIG_SDP_MSPI=y