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Error executing process > 'NFCORE_SMRNASEQ:MIRNA_QUANT:SEQCLUSTER_COLLAPSE #485

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sajjadasaf opened this issue Dec 4, 2024 · 3 comments
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bug Something isn't working

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@sajjadasaf
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Description of the bug

ERROR ~ Error executing process > 'NFCORE_SMRNASEQ:MIRNA_QUANT:SEQCLUSTER_COLLAPSE (Treatment1_REP1)'

Caused by:
Process NFCORE_SMRNASEQ:MIRNA_QUANT:SEQCLUSTER_COLLAPSE (Treatment1_REP1) terminated with an error exit status (137)

Command executed:

seqcluster
collapse
-m 1 --min_size 15
-f Treatment1_REP1.fastp.fastq.gz
-o collapsed

gzip collapsed/_trimmed.fastq
mv collapsed/
_trimmed.fastq.gz Treatment1_REP1_seqcluster.fastq.gz

cat <<-END_VERSIONS > versions.yml
"NFCORE_SMRNASEQ:MIRNA_QUANT:SEQCLUSTER_COLLAPSE":
seqcluster: $(echo $(seqcluster --version 2>&1) | sed 's/^.*seqcluster //')
END_VERSIONS

Command exit status:
137

Command output:
(empty)

Command error:
INFO Run collapse
.command.sh: line 12: 33 Killed seqcluster collapse -m 1 --min_size 15 -f Treatment1_REP1.fastp.fastq.gz -o collapsed

Work dir:
/home/omic/work/ad/0fef8cefbdeba4f439b4e5c42dba7f

Container:
quay.io/biocontainers/seqcluster:1.2.9--pyh5e36f6f_0

Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named .command.sh

-- Check '.nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting

-- Check '.nextflow.log' file for details
-[nf-core/smrnaseq] Pipeline completed with errors-

Command used and terminal output

nextflow run nf-core/smrnaseq --input ./SmallRNA/samplesheet.csv --outdir ./results --genome TAIR10 --mirtrace_species ath -profile docker

Relevant files

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System information

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@sajjadasaf sajjadasaf added the bug Something isn't working label Dec 4, 2024
@sajjadasaf
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please help me to solve this problem

@atrigila
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atrigila commented Dec 9, 2024

Exit code 137 is a memory issue. The process is likely running out of RAM. Bump the amount for that process in your config:

https://nf-co.re/docs/usage/getting_started/configuration#max-resources

@sajjadasaf
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Thank you for your guidance.

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