From 181039afdf80abb1ebc808908e2cf07935b85992 Mon Sep 17 00:00:00 2001 From: Anabella Trigila <18577080+atrigila@users.noreply.github.com> Date: Tue, 12 Nov 2024 14:43:08 -0300 Subject: [PATCH] Update docs/usage.md MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Nicolás Schcolnicov <90359308+nschcolnicov@users.noreply.github.com> --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 3549542a..c525e7b4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -47,7 +47,7 @@ If MirGeneDB should be used instead it needs to be specified using `--mirgenedb` See examples in: [the test-datasets repository of nf-core](https://github.com/nf-core/test-datasets/tree/smrnaseq/MirGeneDB). -> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences rather than `pri`. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF. +> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences, which originate from the hairpin FASTA, rather than the `pri` sequences, which come from the mature FASTA. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF. ### Genome