From 4c97d970add2de651190f0ce092417bafb2dfa11 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Fri, 13 Jul 2018 14:43:21 +0200 Subject: [PATCH 001/146] Added basic shell of documentation --- docs/README.md | 23 ++--- docs/installation.md | 144 ++++++++++++++++++++++++++++++ docs/usage.md | 206 +++++++++++++++++++++++++++++++++++++++++++ 3 files changed, 358 insertions(+), 15 deletions(-) create mode 100644 docs/installation.md diff --git a/docs/README.md b/docs/README.md index 2b9ad38e..87535411 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,17 +1,10 @@ -# nfcore/smrnaseq Documentation +# nf-core/smrnaseq Results -The nfcore/smrnaseq documentation is split into the following files: +The nf-core/smrnaseq documentation is split into a few different files: - -1. [Installation](installation.md) -2. Pipeline configuration - * [Local installation](configuration/local.md) - * [Amazon Web Services (aws)](configuration/aws.md) - * [Swedish UPPMAX clusters](configuration/uppmax.md) - * [Swedish cs3e Hebbe cluster](configuration/c3se.md) - * [Tübingen QBiC](configuration/qbic.md) - * [CCGA Kiel](configuration/ccga.md) - * [Adding your own system](configuration/adding_your_own.md) -3. [Running the pipeline](usage.md) -4. [Output and how to interpret the results](output.md) -5. [Troubleshooting](troubleshooting.md) +* [`installation.md`](installation.md) + * Pipeline installation and configuration instructions +* [`usage.md`](usage.md) + * Instructions on how to run the nf-core/smrnaseq pipeline +* [`output.md`](output.md) + * Document describing all of the results produced by the pipeline, and how to interpret them. diff --git a/docs/installation.md b/docs/installation.md new file mode 100644 index 00000000..fdd10603 --- /dev/null +++ b/docs/installation.md @@ -0,0 +1,144 @@ +# nf-core/smrnaseq installation + +To start using the nf-core/smrnaseq pipeline, follow the steps below: + +1. [Install Nextflow](#1-install-nextflow) +2. [Install the pipeline](#2-install-the-pipeline) + * [Automatic](#21-automatic) + * [Offline](#22-offline) + * [Development](#23-development) +3. [Pipeline configuration](#3-pipeline-configuration) + * [Software deps: Docker and Singularity](#31-software-deps-docker-and-singularity) + * [Software deps: Bioconda](#32-software-deps-bioconda) + * [Configuration profiles](#33-configuration-profiles) +4. [Reference genomes](#4-reference-genomes) +5. [Appendices](#appendices) + * [Running on UPPMAX](#running-on-uppmax) + +## 1) Install NextFlow +Nextflow runs on most POSIX systems (Linux, Mac OSX etc). It can be installed by running the following commands: + +```bash +# Make sure that Java v8+ is installed: +java -version + +# Install Nextflow +curl -fsSL get.nextflow.io | bash + +# Add Nextflow binary to your PATH: +mv nextflow ~/bin +# OR system-wide installation: +# sudo mv nextflow /usr/local/bin +``` + +See [nextflow.io](https://www.nextflow.io/) for further instructions on how to install and configure Nextflow. + +## 2) Install the pipeline + +#### 2.1) Automatic +This pipeline itself needs no installation - NextFlow will automatically fetch it from GitHub if `nf-core/smrnaseq` is specified as the pipeline name. + +#### 2.2) Offline +The above method requires an internet connection so that Nextflow can download the pipeline files. If you're running on a system that has no internet connection, you'll need to download and transfer the pipeline files manually: + +```bash +# Download the latest release of the pipeline (see https://github.com/nf-core/smrnaseq/releases) +curl -L https://github.com/nf-core/smrnaseq/archive/1.0.zip -o nf-core-smrnaseq-v1.0.zip +unzip nf-core-smrnaseq-v1.0.zip +cd /path/to/my/data +nextflow run /path/to/pipelines/nf-core-smrnaseq-v1.0 [parameters] +``` + +#### 2.3) Development + +If you would like to make changes to the pipeline, it's best to make a fork on GitHub and then clone the files. Once cloned you can run the pipeline directly as above. + + +## 3) Pipeline configuration +By default, the pipeline runs with the `standard` configuration profile. This uses a number of sensible defaults for process requirements and is suitable for running on a simple (if powerful!) basic server. You can see this configuration in [`conf/base.config`](../conf/base.config). + +Be warned of two important points about this default configuration: + +1. The default profile uses the `local` executor + * All jobs are run in the login session. If you're using a simple server, this may be fine. If you're using a compute cluster, this is bad as all jobs will run on the head node. + * See the [nextflow docs](https://www.nextflow.io/docs/latest/executor.html) for information about running with other hardware backends. Most job scheduler systems are natively supported. +2. Nextflow will expect all software to be installed and available on the `PATH` + +#### 3.1) Software deps: Docker and Singularity +Running the pipeline with the option `-with-singularity` or `-with-docker` tells Nextflow to enable either [Singularity](http://singularity.lbl.gov/) or Docker for this run. An image containing all of the software requirements will be automatically fetched and used (https://hub.docker.com/r/nf-core/smrnaseq). + +If running offline with Singularity, you'll need to download and transfer the Singularity image first: + +```bash +singularity pull --name nfcore-smrnaseq-[VERSION].simg shub://nfcore/smrnaseq:[VERSION] +``` + +Once transferred, use `-with-singularity` but specify the path to the image file: + +```bash +nextflow run /path/to/nf-core-smrnaseq -with-singularity /path/to/nfcore-smrnaseq-[VERSION].simg +``` + +#### 3.2) Software deps: bioconda + +If you're unable to use either Docker or Singularity but you have conda installed, you can use the bioconda environment that comes with the pipeline. Running this command will create a new conda environment with all of the required software installed: + +```bash +conda env create -f environment.yml +conda clean -a # Recommended, not essential +source activate nfcore-smrnaseq-1.3 # Name depends on version +``` + +The [`environment.yml`](../environment.yml) file is packaged with the pipeline. Note that you may need to download this file from the [GitHub project page](https://github.com/nf-core/smrnaseq) if nextflow is automatically fetching the pipeline files. Ensure that the bioconda environment file version matches the pipeline version that you run. + + +#### 3.3) Configuration profiles + +Nextflow can be configured to run on a wide range of different computational infrastructures. In addition to the above pipeline-specific parameters it is likely that you will need to define system-specific options. For more information, please see the [Nextflow documentation](https://www.nextflow.io/docs/latest/). + +Whilst most parameters can be specified on the command line, it is usually sensible to create a configuration file for your environment. + +If you are the only person to be running this pipeline, you can create your config file as `~/.nextflow/config` and it will be applied every time you run Nextflow. Alternatively, save the file anywhere and reference it when running the pipeline with `-c path/to/config`. + +If you think that there are other people using the pipeline who would benefit from your configuration (e.g. other common cluster setups), please let us know. We can add a new configuration and profile which can used by specifying `-profile ` when running the pipeline. + +The pipeline comes with several such config profiles - see the installation appendices and usage documentation for more information. + +## 4) Reference Genomes +The nf-core/smrnaseq pipeline needs a reference genome for read alignment. Support for many common genomes is built in if running on UPPMAX or AWS, by using [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/). + +Alternatively, you can supply either a Bismark / BWA reference or a FASTA file. This can be done on the command line (see the [usage docs](usage.md#supplying-reference-indices)). +You can use the same pipeline config framework as used with iGenomes to specify multiple references of your own. Add the paths to your NextFlow config under a relevant id and just specify this id with `--genome ID` when you run the pipeline: + +```nextflow +params { + genomes { + 'YOUR-ID' { + bismark = '/BismarkIndex' + bwa_meth = '/genome.fa' + fasta = '/genome.fa' // used if above is not specified + } + 'OTHER-GENOME' { + // [..] + } + } + // Optional - default genome. Ignored if --genome 'OTHER-GENOME' specified on command line + genome = 'YOUR-ID' +} +``` + + +## Appendices + +#### Running on UPPMAX +To run the pipeline on the [Swedish UPPMAX](https://www.uppmax.uu.se/) clusters (`rackham`, `irma`, `bianca` etc), use the command line flag `-profile uppmax`. This tells Nextflow to submit jobs using the SLURM job executor with Singularity for software dependencies. + +Note that you will need to specify your UPPMAX project ID when running a pipeline. To do this, use the command line flag `--project `. The pipeline will exit with an error message if you try to run it pipeline with the default UPPMAX config profile without a project. + +**Optional Extra:** To avoid having to specify your project every time you run Nextflow, you can add it to your personal Nextflow config file instead. Add this line to `~/.nextflow/config`: + +```nextflow +params.project = 'project_ID' // eg. b2017123 +``` + +Bianca users - see the above docs about using the pipeline and singularity containers offline. diff --git a/docs/usage.md b/docs/usage.md index e69de29b..29bf0e72 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -0,0 +1,206 @@ +# nf-core/smrnaseq Usage + +## Table of contents + +* [Running the pipeline](#running-the-pipeline) +* [Inputs and outputs](#inputs-and-outputs) + * [`--reads`](#--reads) + * [`--outdir`](#--outdir) +* [Software](#software) + * [`-with-singularity`](#-with-singularity) + * [`-with-docker`](#-with-docker) +* [Reference Genomes](#reference-genomes) + * [`--genome`](#--genome) + * [Supplying reference indices](#supplying-reference-indices) + * [`--saveReference`](#--savereference) +* [Adapter Trimming](#adapter-trimming) +* [Preset configurations](#preset-configurations) + * [`-profile`](#-profile) +* [Additional parameters](#additional-parameters) + * [`--saveTrimmed`](#--savetrimmed) + * [`--saveAlignedIntermediates`](#--savealignedintermediates) +* [Job Resources](#job-resources) + * [Automatic resubmission](#automatic-resubmission) + * [Maximum resource requests](#maximum-resource-requests) +* [Other command line parameters](#other-command-line-parameters) + * [`-name`](#-name-single-dash) + * [`-resume`](#-resume-single-dash) + * [`--email`](#--email) + * [`--plaintext_email`](#--plaintext_email) + * [`-c`](#-c-single-dash) + * [`--multiqc_config`](#--multiqc_config) + * [`--project`](#--project) + * [`--clusterOptions`](#--clusteroptions) + + +## Running the pipeline +The main command for running the pipeline is as follows: + +```bash +nextflow run nf-core/smrnaseq [parameters] +``` + +Note that the pipeline will create files in your working directory: + +```bash +work/ # Directory containing the nextflow working files +results/ # Finished results (configurable, see below) +.nextflow_log # Log file from Nextflow +.nextflow/ # Nextflow cache and history information +``` + + +## Inputs and outputs + +### `--reads` +Location of the input FastQ files: + +```bash + --reads 'path/to/data/sample_*_{1,2}.fastq' +``` + +**NB: Must be enclosed in quotes!** + +The file path should be in quotation marks to prevent shell glob expansion. + +If left unspecified, the pipeline will assume that the data is in a directory called `data` in the working directory (`data/*.fastq.gz`). + +### `--outdir` +The output directory where the results will be saved. + +## Software +There are different ways to provide the required software dependencies for the pipeline. The recommended method is to use either Docker or Singularity. The command line flags below are only needed if you are using the `standard` config profile (see below). It is not required with the the other profiles. + +If using Bioconda or manual installation, no command line option is required. + +### `-with-singularity` +Flag to enable use of singularity. The image will automatically be pulled from the internet. If running offline, follow the option with the path to the image file. + +### `-with-docker` +Flag to enable docker. The image will automatically be pulled from Dockerhub. + +## Reference Genomes + +### `--genome` +To make it easier to run an analysis with a common reference genome, the pipeline comes bundled with configuration paths for the AWS-iGenomes resource. If using these references, you can specify just the genome ID using `--genome`. + +The default AWS-iGenome paths are for files hosted on AWS s3. If running regularly, it is recommended that you download your own copy of the reference genomes and set the path root using `--igenomes_base` (or `params.igenomes_base` in a config file). + +If using the `uppmax` config profile (see below), the iGenomes base is already set to a local copy of the files held on the UPPMAX clusters. + +See [`conf/igenomes.config`](conf/igenomes.config) for a list of all of the supported reference genomes and their keys. Common genomes that are supported are: + +* Human + * `--genome GRCh37` +* Mouse + * `--genome GRCm38` +* _Drosophila_ + * `--genome BDGP6` +* _S. cerevisiae_ + * `--genome 'R64-1-1'` + +> There are numerous others - check the config file for more. + +### Supplying reference indices + +If you don't want to use the illumina iGenomes references, you can supply your own reference genome. + + + + +### `--saveReference` +Supply this parameter to save any generated reference genome files to your results folder. These can then be used for future pipeline runs, reducing processing times. + +## Adapter Trimming +Bisulfite libraries often require additional base pairs to be removed from the ends of the reads before alignment. You can specify these custom trimming parameters as follows: + +* `--clip_r1 ` + * Instructs Trim Galore to remove bp from the 5' end of read 1 (or single-end reads). +* `--clip_r2 ` + * Instructs Trim Galore to remove bp from the 5' end of read 2 (paired-end reads only). +* `--three_prime_clip_r1 ` + * Instructs Trim Galore to remove bp from the 3' end of read 1 _AFTER_ adapter/quality trimming has been +* `--three_prime_clip_r2 ` + * Instructs Trim Galore to re move bp from the 3' end of read 2 _AFTER_ adapter/quality trimming has been performed. + +## Preset configurations + +### `-profile` +Use this parameter to choose a configuration profile. See the [installation documentation](installation.md#33-configuration-profiles) for more information about profiles. + +Profiles available with the pipeline are: + +* `standard` + * The default profile, used if `-profile` is not specified. + * Uses sensible defaults for requirements, runs using the `local` executor (native system calls) and expects all software to be installed and available on the `PATH`. + * This profile is mainly designed to be used as a starting point for other configurations and is inherited by most of the other profiles below. +* `uppmax` + * Designed to be used on the Swedish [UPPMAX](http://uppmax.uu.se/) clusters such as `milou`, `rackham`, `bianca` and `irma` + * Launches jobs using the SLURM executor. + * Uses [Singularity](http://singularity.lbl.gov/) to provide all software requirements + * Comes with locations for illumina iGenome reference paths built in + * Use with `--project` to provide your UPPMAX project ID. +* `uppmax_devel` + * Uses the milou [devel partition](http://www.uppmax.uu.se/support/user-guides/slurm-user-guide/#tocjump_030509106905141747_8) for testing the pipeline quickly. + * Not suitable for proper analysis runs +* `aws` + * A starter configuration for running the pipeline on Amazon Web Services. + * Specifies docker configuration and uses the `spark` job executor + * Requires additional configuration to run - see the documentation dedicated to this topic. +* `none` + * No configuration at all. Useful if you want to build your own config from scratch and want to avoid loading in the default `base` config profile. + +## Additional parameters + +### `--saveTrimmed` +By default, trimmed FastQ files will not be saved to the results directory. Specify this flag (or set to true in your config file) to copy these files to the results directory when complete. + +### `--saveAlignedIntermediates` +By default intermediate BAM files will not be saved. The final BAM files created after the deduplication step are always. Set to true to also copy out BAM files from the initial Bismark alignment step. If `--nodedup` or `--rrbs` is specified then BAMs from the initial alignment will always be saved. + +## Job Resources +### Automatic resubmission +Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits on UPPMAX with an error code of `143` or `137` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. + +### Maximum resource requests +All resource requests are checked against the following default limits (`standard` config profile shown): + +```bash +--max_memory '128.GB' +--max_cpus '16' +--max_time '240.h' +``` + +If a task requests more than this amount, it will be reduced to this threshold. + +To adjust these limits, specify them on the command line, eg. `--max_memory '64.GB'`. + +Note that these limits are the maximum to be used _per task_. Nextflow will automatically attempt to parallelise as many jobs as possible given the available resources. + +## Other command line parameters + +### `-name` _(single dash)_ +Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. This name is also used in MultiQC reports if specified. + +### `-resume` _(single dash)_ +Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. + +You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. + +### `--email` +Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to speicfy this on the command line for every run. + +### `--plaintext_email` +Set to receive plain-text e-mails instead of HTML formatted. + +### `-c` _(single dash)_ +Specify the path to a specific config file (this is a core NextFlow command). You can use this in combination with configuration profiles to override defaults. + +### `--multiqc_config` +If you would like to supply a custom config file to MultiQC, you can specify a path with `--multiqc_config`. This is used _instead of_ the [config file](../conf/multiqc_config.yaml) that comes with the pipeline. + +### `--project` +UPPMAX profile only: Cluster project for SLURM job submissions. + +### `--clusterOptions` +UPPMAX profile only: Submit arbitrary SLURM options. From b6f1177c31b0fd7ef7cadfdf5e618cdbdbf05c1e Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Thu, 20 Jun 2019 17:18:12 -0400 Subject: [PATCH 002/146] fix authors --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 98d87bb6..de77663e 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,5 +1,5 @@ FROM nfcore/base -LABEL authors="Phil Ewels " \ +LABEL authors="Alexander Peltzer " \ description="Docker image containing all requirements for nf-core/smrnaseq pipeline" COPY environment.yml / From b21a7da869d7c97a1a52ca3b77f6d06cc60d92a1 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Thu, 20 Jun 2019 17:18:23 -0400 Subject: [PATCH 003/146] fix authors --- README.md | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 39f3633f..90608424 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,6 @@ # nf-core/smrnaseq -**Small-RNA-Seq Best Practice Analysis Pipeline. Started May 2016.**. +**Small-RNA-Seq Best Practice Analysis Pipeline**. [![Build Status](https://travis-ci.com/nf-core/smrnaseq.svg?branch=master)](https://travis-ci.com/nf-core/smrnaseq) [![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A50.32.0-brightgreen.svg)](https://www.nextflow.io/) @@ -27,4 +27,6 @@ The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found ## Credits -nf-core/smrnaseq was originally written by Phil Ewels , Chuan Wang , Rickard Hammarén +nf-core/smrnaseq was originally written for use at the [National Genomics Infrastructure](https://portal.scilifelab.se/genomics/) at [SciLifeLab](http://www.scilifelab.se/) in Stockholm, Sweden, by Phil Ewels (@ewels), Chuan Wang (@chuan-wang) and Rickard Hammarén (@Hammarn). + +It is been updated by Lorena Pantano (@lpantano). \ No newline at end of file From dcb5e8569f70c1be86690dd234934b8f1999e02c Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 25 Jun 2019 14:08:03 -0400 Subject: [PATCH 004/146] fix title --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index bd8b2952..248bf783 100644 --- a/nextflow.config +++ b/nextflow.config @@ -87,7 +87,7 @@ manifest { name = 'nf-core/smrnaseq' author = 'Phil Ewels ' homePage = 'https://github.com/nf-core/smrnaseq' - description = 'Small-RNA-Seq Best Practice Analysis Pipeline. Started May 2016.' + description = 'Small-RNA-Seq Best Practice Analysis Pipeline' mainScript = 'main.nf' nextflowVersion = '>=0.32.0' version = '1.0dev' From fff18bee3ed5d7340493eaa585fc4366ee0fbfad Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 25 Jun 2019 14:09:45 -0400 Subject: [PATCH 005/146] fix manifest --- nextflow.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 248bf783..6fef9df8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -85,9 +85,9 @@ dag { manifest { name = 'nf-core/smrnaseq' - author = 'Phil Ewels ' + author = 'Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano ' homePage = 'https://github.com/nf-core/smrnaseq' - description = 'Small-RNA-Seq Best Practice Analysis Pipeline' + description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' nextflowVersion = '>=0.32.0' version = '1.0dev' From 8bf75acebfa1b8b9a14620db82918e6612fe1261 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Thu, 27 Jun 2019 09:50:26 -0400 Subject: [PATCH 006/146] fix markdown lint --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 90608424..fc070dcd 100644 --- a/README.md +++ b/README.md @@ -29,4 +29,4 @@ The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found ## Credits nf-core/smrnaseq was originally written for use at the [National Genomics Infrastructure](https://portal.scilifelab.se/genomics/) at [SciLifeLab](http://www.scilifelab.se/) in Stockholm, Sweden, by Phil Ewels (@ewels), Chuan Wang (@chuan-wang) and Rickard Hammarén (@Hammarn). -It is been updated by Lorena Pantano (@lpantano). \ No newline at end of file +It is been updated by Lorena Pantano (@lpantano). From 1a99cd6e57ac4a40e57931cab1e0ddf95c16646d Mon Sep 17 00:00:00 2001 From: KevinMenden Date: Thu, 16 Jan 2020 07:38:32 +0100 Subject: [PATCH 007/146] small doc fix --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 384bcd90..951648fb 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -144,7 +144,7 @@ Default: "illumina" | illumina | Illumina TruSeq Small RNA | clip_R1 = 0; three_prime_clip_R1 = 0 | TGGAATTCTCGGGTGCCAAGG | | nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | clip_R1 = 4; three_prime_clip_R1 = 4 | TGGAATTCTCGGGTGCCAAGG | | qiaseq | QIAGEN QIAseq miRNA | clip_R1 = 0; three_prime_clip_R1 = 0 | AACTGTAGGCACCATCAAT | -| cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | GATCGGAAGAGCACACGTCTG | +| cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | AAAAAAAAAAA + GATCGGAAGAGCACACGTCTG (only polyA is used for trimming) | ## Reference genomes From 7b0bfd82d5184e016c47bf31a63b61a188e885ef Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Mon, 10 Feb 2020 21:53:46 -0500 Subject: [PATCH 008/146] make sure one file is used in collapse step this is related to UMI in the fastq files. Address: https://github.com/nf-core/smrnaseq/issues/35 --- main.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index f400b181..ca2085e2 100644 --- a/main.nf +++ b/main.nf @@ -396,13 +396,14 @@ process collapse { file reads from trimmed_reads_collapse output: - file 'collapsed/*.fastq' into collapsed_fasta + file 'final/*.fastq' into collapsed_fasta script: prefix = reads.toString() - '_trimmed.fq.gz' """ seqcluster collapse -f $reads -m 1 --min_size 15 -o collapsed - mv collapsed/${prefix}_trimmed_trimmed.fastq collapsed/${prefix}.fastq + mkdir final + mv collapsed/${prefix}_trimmed_trimmed.fastq final/${prefix}.fastq """ } From 496980987844f2b1b9fa58326ddedb50ae03ea4b Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Mon, 10 Feb 2020 21:57:13 -0500 Subject: [PATCH 009/146] add changelog updates and new version --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ac0e756c..cc395e44 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,5 +1,8 @@ # nf-core/smrnaseq: Changelog +## v1.0.1dev +* Fix error when UMI is on the reads header: https://github.com/nf-core/smrnaseq/issues/35 + ## v1.0.0 - 2019-09-19 * Add figures to output documentation * Add samtools stats for genome alignments From 2b3e647a7da59248ee39c758228334af32ddf52f Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Tue, 11 Feb 2020 15:18:46 +0100 Subject: [PATCH 010/146] add social preview image --- assets/nf-core-smrnaseq_social_preview.png | Bin 0 -> 47299 bytes assets/nf-core-smrnaseq_social_preview.svg | 448 +++++++++++++++++++++ 2 files changed, 448 insertions(+) create mode 100644 assets/nf-core-smrnaseq_social_preview.png create mode 100644 assets/nf-core-smrnaseq_social_preview.svg diff --git a/assets/nf-core-smrnaseq_social_preview.png b/assets/nf-core-smrnaseq_social_preview.png new file mode 100644 index 0000000000000000000000000000000000000000..cd6f354d673b29868beaf1f1092b8746eaa4f13a GIT binary patch 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zAN=p*`R}6m?^eJQ_-~|Oky}~_jQ_^R|Eu)za&eWWb + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + image/svg+xml + + + + + + + A small-RNA sequencing analysis pipeline + smrnaseq + + + + + + + + + + + + + + + + + + + + + + + + + From 4e823c9b919791332a4df7486b1a49b9c074365e Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 12 Feb 2020 16:50:59 +0100 Subject: [PATCH 011/146] update CHANGELOG --- CHANGELOG.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ac0e756c..dc8e5290 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,6 +1,7 @@ # nf-core/smrnaseq: Changelog ## v1.0.0 - 2019-09-19 + * Add figures to output documentation * Add samtools stats for genome alignments * Add seqkit and remove razers @@ -9,6 +10,7 @@ * Update tools and Dockerfile/Singularity to match current template #### Dependency Updates + * openjdk 8.0.144 -> 11.0.1 * fastqc 0.11.7 -> 0.11.8 * trim-galore 0.5.0 -> 0.6.2 @@ -32,11 +34,13 @@ * bioconductor-limma=3.40.0 -> 3.40.2 ## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2019-01-10 + * Add "protocol" with pre-defined settings * Add miRTrace in the pipeline. * Software updates: multiqc 1.6 to 1.7. ## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2018-08-06 + * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. * Add option for sequencing centre in BAM file. From acf380a07db0b00848811d5d840452cfea21d1ea Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 12 Feb 2020 16:54:36 +0100 Subject: [PATCH 012/146] remove links to non-existing 1.0 releases --- CHANGELOG.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index dc8e5290..f034e211 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -33,13 +33,13 @@ * bioconductor-edger=3.26.0 -> 3.26.5 * bioconductor-limma=3.40.0 -> 3.40.2 -## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2019-01-10 +## 1.0 - 2019-01-10 * Add "protocol" with pre-defined settings * Add miRTrace in the pipeline. * Software updates: multiqc 1.6 to 1.7. -## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2018-08-06 +## 1.0 - 2018-08-06 * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. From 3a71bc9b8a45c8e82f8da25a5c72269363681809 Mon Sep 17 00:00:00 2001 From: runner Date: Thu, 20 Feb 2020 15:34:02 +0000 Subject: [PATCH 013/146] Template update for nf-core/tools version 1.9 --- .github/CONTRIBUTING.md | 52 ++- .github/ISSUE_TEMPLATE/bug_report.md | 43 ++- .github/ISSUE_TEMPLATE/feature_request.md | 18 +- .github/PULL_REQUEST_TEMPLATE.md | 28 +- .github/markdownlint.yml | 4 - .github/workflows/branch.yml | 16 + .github/workflows/ci.yml | 30 ++ .github/workflows/linting.yml | 50 +++ .gitignore | 3 +- .travis.yml | 42 -- CHANGELOG.md | 14 +- CODE_OF_CONDUCT.md | 2 +- Dockerfile | 14 +- LICENSE | 2 +- README.md | 57 ++- assets/email_template.html | 4 +- assets/email_template.txt | 12 +- assets/multiqc_config.yaml | 4 +- assets/nf-core-smrnaseq_logo.png | Bin 0 -> 12974 bytes assets/sendmail_template.txt | 17 + bin/markdown_to_html.py | 100 +++++ bin/markdown_to_html.r | 51 --- bin/scrape_software_versions.py | 15 +- conf/awsbatch.config | 18 - conf/base.config | 37 +- conf/igenomes.config | 451 +++++++++++++++++----- conf/test.config | 7 +- docs/images/nf-core-smrnaseq_logo.png | Bin 0 -> 22048 bytes docs/output.md | 4 +- docs/usage.md | 95 +++-- environment.yml | 10 +- main.nf | 278 +++++++------ nextflow.config | 56 ++- 33 files changed, 1053 insertions(+), 481 deletions(-) create mode 100644 .github/workflows/branch.yml create mode 100644 .github/workflows/ci.yml create mode 100644 .github/workflows/linting.yml delete mode 100644 .travis.yml create mode 100644 assets/nf-core-smrnaseq_logo.png create mode 100755 bin/markdown_to_html.py delete mode 100755 bin/markdown_to_html.r delete mode 100644 conf/awsbatch.config create mode 100644 docs/images/nf-core-smrnaseq_logo.png diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 2b78eccc..0562bf83 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,47 +1,57 @@ # nf-core/smrnaseq: Contributing Guidelines -Hi there! Many thanks for taking an interest in improving nf-core/smrnaseq. +Hi there! +Many thanks for taking an interest in improving nf-core/smrnaseq. -We try to manage the required tasks for nf-core/smrnaseq using GitHub issues, you probably came to this page when creating one. Please use the pre-filled template to save time. - -However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) - -> If you need help using or modifying nf-core/smrnaseq then the best place to ask is on the pipeline channel on [Slack](https://nf-core-invite.herokuapp.com/). +We try to manage the required tasks for nf-core/smrnaseq using GitHub issues, you probably came to this page when creating one. +Please use the pre-filled template to save time. +However, don't be put off by this template - other more general issues and suggestions are welcome! +Contributions to the code are even more welcome ;) +> If you need help using or modifying nf-core/smrnaseq then the best place to ask is on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow -If you'd like to write some code for nf-core/smrnaseq, the standard workflow -is as follows: -1. Check that there isn't already an issue about your idea in the - [nf-core/smrnaseq issues](https://github.com/nf-core/smrnaseq/issues) to avoid - duplicating work. +If you'd like to write some code for nf-core/smrnaseq, the standard workflow is as follows: + +1. Check that there isn't already an issue about your idea in the [nf-core/smrnaseq issues](https://github.com/nf-core/smrnaseq/issues) to avoid duplicating work * If there isn't one already, please create one so that others know you're working on this -2. Fork the [nf-core/smrnaseq repository](https://github.com/nf-core/smrnaseq) to your GitHub account +2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/smrnaseq repository](https://github.com/nf-core/smrnaseq) to your GitHub account 3. Make the necessary changes / additions within your forked repository -4. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged. - -If you're not used to this workflow with git, you can start with some [basic docs from GitHub](https://help.github.com/articles/fork-a-repo/) or even their [excellent interactive tutorial](https://try.github.io/). +4. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged +If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). ## Tests -When you create a pull request with changes, [Travis CI](https://travis-ci.org/) will run automatic tests. + +When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. There are typically two types of tests that run: ### Lint Tests -The nf-core has a [set of guidelines](http://nf-co.re/guidelines) which all pipelines must adhere to. + +`nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. If any failures or warnings are encountered, please follow the listed URL for more documentation. ### Pipeline Tests -Each nf-core pipeline should be set up with a minimal set of test-data. -Travis CI then runs the pipeline on this data to ensure that it exists successfully. + +Each `nf-core` pipeline should be set up with a minimal set of test-data. +`GitHub Actions` then runs the pipeline on this data to ensure that it exits successfully. If there are any failures then the automated tests fail. -These tests are run both with the latest available version of Nextflow and also the minimum required version that is stated in the pipeline code. +These tests are run both with the latest available version of `Nextflow` and also the minimum required version that is stated in the pipeline code. + +## Patch + +: warning: Only in the unlikely and regretful event of a release happening with a bug. + +* On your own fork, make a new branch `patch` based on `upstream/master`. +* Fix the bug, and bump version (X.Y.Z+1). +* A PR should be made on `master` from patch to directly this particular bug. ## Getting help -For further information/help, please consult the [nf-core/smrnaseq documentation](https://github.com/nf-core/smrnaseq#documentation) and don't hesitate to get in touch on the pipeline channel on [Slack](https://nf-core-invite.herokuapp.com/). + +For further information/help, please consult the [nf-core/smrnaseq documentation](https://nf-co.re/nf-core/smrnaseq/docs) and don't hesitate to get in touch on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 73f22bdd..2857708e 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -1,31 +1,42 @@ +# nf-core/smrnaseq bug report + Hi there! -Thanks for telling us about a problem with the pipeline. Please delete this text and anything that's not relevant from the template below: +Thanks for telling us about a problem with the pipeline. +Please delete this text and anything that's not relevant from the template below: + +## Describe the bug -#### Describe the bug A clear and concise description of what the bug is. -#### Steps to reproduce +## Steps to reproduce + Steps to reproduce the behaviour: + 1. Command line: `nextflow run ...` 2. See error: _Please provide your error message_ -#### Expected behaviour +## Expected behaviour + A clear and concise description of what you expected to happen. -#### System: - - Hardware: [e.g. HPC, Desktop, Cloud...] - - Executor: [e.g. slurm, local, awsbatch...] - - OS: [e.g. CentOS Linux, macOS, Linux Mint...] - - Version [e.g. 7, 10.13.6, 18.3...] +## System + +- Hardware: +- Executor: +- OS: +- Version + +## Nextflow Installation + +- Version: + +## Container engine -#### Nextflow Installation: - - Version: [e.g. 0.31.0] +- Engine: +- version: +- Image tag: -#### Container engine: - - Engine: [e.g. Conda, Docker or Singularity] - - version: [e.g. 1.0.0] - - Image tag: [e.g. nfcore/smrnaseq:1.0.0] +## Additional context -#### Additional context Add any other context about the problem here. diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index 1f025b77..cf19a32d 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,16 +1,24 @@ +# nf-core/smrnaseq feature request + Hi there! -Thanks for suggesting a new feature for the pipeline! Please delete this text and anything that's not relevant from the template below: +Thanks for suggesting a new feature for the pipeline! +Please delete this text and anything that's not relevant from the template below: + +## Is your feature request related to a problem? Please describe -#### Is your feature request related to a problem? Please describe. A clear and concise description of what the problem is. + Ex. I'm always frustrated when [...] -#### Describe the solution you'd like +## Describe the solution you'd like + A clear and concise description of what you want to happen. -#### Describe alternatives you've considered +## Describe alternatives you've considered + A clear and concise description of any alternative solutions or features you've considered. -#### Additional context +## Additional context + Add any other context about the feature request here. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 6e48fa56..9db6d8f3 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,15 +1,19 @@ -Many thanks to contributing to nf-core/smrnaseq! +# nf-core/smrnaseq pull request -Please fill in the appropriate checklist below (delete whatever is not relevant). These are the most common things requested on pull requests (PRs). +Many thanks for contributing to nf-core/smrnaseq! + +Please fill in the appropriate checklist below (delete whatever is not relevant). +These are the most common things requested on pull requests (PRs). ## PR checklist - - [ ] This comment contains a description of changes (with reason) - - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If necessary, also make a PR on the [nf-core/smrnaseq branch on the nf-core/test-datasets repo]( https://github.com/nf-core/test-datasets/pull/new/nf-core/smrnaseq) - - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - - [ ] Make sure your code lints (`nf-core lint .`). - - [ ] Documentation in `docs` is updated - - [ ] `CHANGELOG.md` is updated - - [ ] `README.md` is updated - -**Learn more about contributing:** https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md + +- [ ] This comment contains a description of changes (with reason) +- [ ] If you've fixed a bug or added code that should be tested, add tests! +- [ ] If necessary, also make a PR on the [nf-core/smrnaseq branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/smrnaseq) +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). +- [ ] Make sure your code lints (`nf-core lint .`). +- [ ] Documentation in `docs` is updated +- [ ] `CHANGELOG.md` is updated +- [ ] `README.md` is updated + +**Learn more about contributing:** [CONTRIBUTING.md](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) \ No newline at end of file diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml index e052a635..96b12a70 100644 --- a/.github/markdownlint.yml +++ b/.github/markdownlint.yml @@ -1,9 +1,5 @@ # Markdownlint configuration file default: true, line-length: false -no-multiple-blanks: 0 -blanks-around-headers: false -blanks-around-lists: false -header-increment: false no-duplicate-header: siblings_only: true diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml new file mode 100644 index 00000000..819d8516 --- /dev/null +++ b/.github/workflows/branch.yml @@ -0,0 +1,16 @@ +name: nf-core branch protection +# This workflow is triggered on PRs to master branch on the repository +# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev` +on: + pull_request: + branches: + - master + +jobs: + test: + runs-on: ubuntu-18.04 + steps: + # PRs are only ok if coming from an nf-core `dev` branch or a fork `patch` branch + - name: Check PRs + run: | + { [[ $(git remote get-url origin) == *nf-core/smrnaseq ]] && [[ ${GITHUB_HEAD_REF} = "dev" ]]; } || [[ ${GITHUB_HEAD_REF} == "patch" ]] diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml new file mode 100644 index 00000000..77d552a2 --- /dev/null +++ b/.github/workflows/ci.yml @@ -0,0 +1,30 @@ +name: nf-core CI +# This workflow is triggered on pushes and PRs to the repository. +# It runs the pipeline with the minimal test dataset to check that it completes without any syntax errors +on: [push, pull_request] + +jobs: + test: + env: + NXF_VER: ${{ matrix.nxf_ver }} + NXF_ANSI_LOG: false + runs-on: ubuntu-latest + strategy: + matrix: + # Nextflow versions: check pipeline minimum and current latest + nxf_ver: ['19.10.0', ''] + steps: + - uses: actions/checkout@v2 + - name: Install Nextflow + run: | + wget -qO- get.nextflow.io | bash + sudo mv nextflow /usr/local/bin/ + - name: Pull docker image + run: | + docker pull nfcore/smrnaseq:dev + docker tag nfcore/smrnaseq:dev nfcore/smrnaseq:dev + - name: Run pipeline with test data + run: | + # TODO nf-core: You can customise CI pipeline run tests as required + # (eg. adding multiple test runs with different parameters) + nextflow run ${GITHUB_WORKSPACE} -profile test,docker diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml new file mode 100644 index 00000000..1e0827a8 --- /dev/null +++ b/.github/workflows/linting.yml @@ -0,0 +1,50 @@ +name: nf-core linting +# This workflow is triggered on pushes and PRs to the repository. +# It runs the `nf-core lint` and markdown lint tests to ensure that the code meets the nf-core guidelines +on: + push: + pull_request: + release: + types: [published] + +jobs: + Markdown: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v2 + - uses: actions/setup-node@v1 + with: + node-version: '10' + - name: Install markdownlint + run: npm install -g markdownlint-cli + - name: Run Markdownlint + run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + YAML: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v1 + - uses: actions/setup-node@v1 + with: + node-version: '10' + - name: Install yaml-lint + run: npm install -g yaml-lint + - name: Run yaml-lint + run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml") + nf-core: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v2 + - name: Install Nextflow + run: | + wget -qO- get.nextflow.io | bash + sudo mv nextflow /usr/local/bin/ + - uses: actions/setup-python@v1 + with: + python-version: '3.6' + architecture: 'x64' + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install nf-core + - name: Run nf-core lint + run: nf-core lint ${GITHUB_WORKSPACE} diff --git a/.gitignore b/.gitignore index 5b54e3e6..6354f370 100644 --- a/.gitignore +++ b/.gitignore @@ -3,5 +3,6 @@ work/ data/ results/ .DS_Store -tests/test_data +tests/ +testing/ *.pyc diff --git a/.travis.yml b/.travis.yml deleted file mode 100644 index a1a32657..00000000 --- a/.travis.yml +++ /dev/null @@ -1,42 +0,0 @@ -sudo: required -language: python -jdk: openjdk8 -services: docker -python: '3.6' -cache: pip -matrix: - fast_finish: true - -before_install: - # PRs to master are only ok if coming from dev branch - - '[ $TRAVIS_PULL_REQUEST = "false" ] || [ $TRAVIS_BRANCH != "master" ] || ([ $TRAVIS_PULL_REQUEST_SLUG = $TRAVIS_REPO_SLUG ] && [ $TRAVIS_PULL_REQUEST_BRANCH = "dev" ])' - # Pull the docker image first so the test doesn't wait for this - - docker pull nfcore/smrnaseq:dev - # Fake the tag locally so that the pipeline runs properly - # Looks weird when this is :dev to :dev, but makes sense when testing code for a release (:dev to :1.0.1) - - docker tag nfcore/smrnaseq:dev nfcore/smrnaseq:dev - -install: - # Install Nextflow - - mkdir /tmp/nextflow && cd /tmp/nextflow - - wget -qO- get.nextflow.io | bash - - sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow - # Install nf-core/tools - - pip install --upgrade pip - - pip install nf-core - # Reset - - mkdir ${TRAVIS_BUILD_DIR}/tests && cd ${TRAVIS_BUILD_DIR}/tests - # Install markdownlint-cli - - sudo apt-get install npm && npm install -g markdownlint-cli - -env: - - NXF_VER='0.32.0' # Specify a minimum NF version that should be tested and work - - NXF_VER='' # Plus: get the latest NF version and check that it works - -script: - # Lint the pipeline code - - nf-core lint ${TRAVIS_BUILD_DIR} - # Lint the documentation - - markdownlint ${TRAVIS_BUILD_DIR} -c ${TRAVIS_BUILD_DIR}/.github/markdownlint.yml - # Run the pipeline with the test profile - - nextflow run ${TRAVIS_BUILD_DIR} -profile test,docker diff --git a/CHANGELOG.md b/CHANGELOG.md index 95eea151..668b314e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,4 +1,16 @@ # nf-core/smrnaseq: Changelog -## v1.0dev - [date] +The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/) +and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html). + +## v1.0.0 - [date] + Initial release of nf-core/smrnaseq, created with the [nf-core](http://nf-co.re/) template. + +### `Added` + +### `Fixed` + +### `Dependencies` + +### `Deprecated` diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index 09226d0d..cf930c8a 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -34,7 +34,7 @@ This Code of Conduct applies both within project spaces and in public spaces whe ## Enforcement -Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-core-invite.herokuapp.com/). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately. +Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-co.re/join/slack). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately. Project maintainers who do not follow or enforce the Code of Conduct in good faith may face temporary or permanent repercussions as determined by other members of the project's leadership. diff --git a/Dockerfile b/Dockerfile index de77663e..12804ea0 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,7 +1,13 @@ -FROM nfcore/base -LABEL authors="Alexander Peltzer " \ - description="Docker image containing all requirements for nf-core/smrnaseq pipeline" +FROM nfcore/base:1.9 +LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ + description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" +# Install the conda environment COPY environment.yml / RUN conda env create -f /environment.yml && conda clean -a -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0dev/bin:$PATH + +# Add conda installation dir to PATH (instead of doing 'conda activate') +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.0/bin:$PATH + +# Dump the details of the installed packages to a file for posterity +RUN conda env export --name nf-core-smrnaseq-1.0.0 > nf-core-smrnaseq-1.0.0.yml diff --git a/LICENSE b/LICENSE index 9c60c99c..ba7a3227 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Phil Ewels +Copyright (c) Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index fc070dcd..380046e0 100644 --- a/README.md +++ b/README.md @@ -1,18 +1,44 @@ -# nf-core/smrnaseq +# ![nf-core/smrnaseq](docs/images/nf-core-smrnaseq_logo.png) -**Small-RNA-Seq Best Practice Analysis Pipeline**. +**Small RNA-Seq Best Practice Analysis Pipeline.**. -[![Build Status](https://travis-ci.com/nf-core/smrnaseq.svg?branch=master)](https://travis-ci.com/nf-core/smrnaseq) -[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A50.32.0-brightgreen.svg)](https://www.nextflow.io/) +[![GitHub Actions CI Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/smrnaseq/actions) +[![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions) +[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/) [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/smrnaseq.svg)](https://hub.docker.com/r/nfcore/smrnaseq) ## Introduction + The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. +## Quick Start + +i. Install [`nextflow`](https://nf-co.re/usage/installation) + +ii. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility (please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles)) + +iii. Download the pipeline and test it on a minimal dataset with a single command + +```bash +nextflow run nf-core/smrnaseq -profile test, +``` + +> Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + +iv. Start running your own analysis! + + + +```bash +nextflow run nf-core/smrnaseq -profile --reads '*_R{1,2}.fastq.gz' --genome GRCh37 +``` + +See [usage docs](docs/usage.md) for all of the available options when running the pipeline. ## Documentation + The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found in the `docs/` directory: 1. [Installation](https://nf-co.re/usage/installation) @@ -27,6 +53,25 @@ The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found ## Credits -nf-core/smrnaseq was originally written for use at the [National Genomics Infrastructure](https://portal.scilifelab.se/genomics/) at [SciLifeLab](http://www.scilifelab.se/) in Stockholm, Sweden, by Phil Ewels (@ewels), Chuan Wang (@chuan-wang) and Rickard Hammarén (@Hammarn). -It is been updated by Lorena Pantano (@lpantano). +nf-core/smrnaseq was originally written by Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano . + +## Contributions and Support + +If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). + +For further information or help, don't hesitate to get in touch on [Slack](https://nfcore.slack.com/channels/smrnaseq) (you can join with [this invite](https://nf-co.re/join/slack)). + +## Citation + + + + +You can cite the `nf-core` publication as follows: + +> **The nf-core framework for community-curated bioinformatics pipelines.** +> +> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. +> +> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). +> ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) diff --git a/assets/email_template.html b/assets/email_template.html index 45a9195f..852ea84a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -5,12 +5,14 @@ - + nf-core/smrnaseq Pipeline Report
+ +

nf-core/smrnaseq v${version}

Run Name: $runName

diff --git a/assets/email_template.txt b/assets/email_template.txt index 92b9ca20..6e8b6393 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -1,6 +1,12 @@ -======================================== - nf-core/smrnaseq v${version} -======================================== +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/smrnaseq v${version} +---------------------------------------------------- + Run Name: $runName <% if (success){ diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml index 5ef712bc..9e05960a 100644 --- a/assets/multiqc_config.yaml +++ b/assets/multiqc_config.yaml @@ -3,7 +3,9 @@ report_comment: > analysis pipeline. 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HcmV?d00001 diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 2d671220..83d8a06e 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -8,6 +8,23 @@ Content-Type: text/html; charset=utf-8 $email_html +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-smrnaseq_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-smrnaseq_logo.png" + +<% out << new File("$baseDir/assets/nf-core-smrnaseq_logo.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + <% if (mqcFile){ def mqcFileObj = new File("$mqcFile") diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py new file mode 100755 index 00000000..57cc4263 --- /dev/null +++ b/bin/markdown_to_html.py @@ -0,0 +1,100 @@ +#!/usr/bin/env python +from __future__ import print_function +import argparse +import markdown +import os +import sys + +def convert_markdown(in_fn): + input_md = open(in_fn, mode="r", encoding="utf-8").read() + html = markdown.markdown( + "[TOC]\n" + input_md, + extensions = [ + 'pymdownx.extra', + 'pymdownx.b64', + 'pymdownx.highlight', + 'pymdownx.emoji', + 'pymdownx.tilde', + 'toc' + ], + extension_configs = { + 'pymdownx.b64': { + 'base_path': os.path.dirname(in_fn) + }, + 'pymdownx.highlight': { + 'noclasses': True + }, + 'toc': { + 'title': 'Table of Contents' + } + } + ) + return html + +def wrap_html(contents): + header = """ + + + + + +
+ """ + footer = """ +
+ + + """ + return header + contents + footer + + +def parse_args(args=None): + parser = argparse.ArgumentParser() + parser.add_argument('mdfile', type=argparse.FileType('r'), nargs='?', + help='File to convert. Defaults to stdin.') + parser.add_argument('-o', '--out', type=argparse.FileType('w'), + default=sys.stdout, + help='Output file name. Defaults to stdout.') + return parser.parse_args(args) + +def main(args=None): + args = parse_args(args) + converted_md = convert_markdown(args.mdfile.name) + html = wrap_html(converted_md) + args.out.write(html) + +if __name__ == '__main__': + sys.exit(main()) diff --git a/bin/markdown_to_html.r b/bin/markdown_to_html.r deleted file mode 100755 index abe13350..00000000 --- a/bin/markdown_to_html.r +++ /dev/null @@ -1,51 +0,0 @@ -#!/usr/bin/env Rscript - -# Command line argument processing -args = commandArgs(trailingOnly=TRUE) -if (length(args) < 2) { - stop("Usage: markdown_to_html.r ", call.=FALSE) -} -markdown_fn <- args[1] -output_fn <- args[2] - -# Load / install packages -if (!require("markdown")) { - install.packages("markdown", dependencies=TRUE, repos='http://cloud.r-project.org/') - library("markdown") -} - -base_css_fn <- getOption("markdown.HTML.stylesheet") -base_css <- readChar(base_css_fn, file.info(base_css_fn)$size) -custom_css <- paste(base_css, " -body { - padding: 3em; - margin-right: 350px; - max-width: 100%; -} -#toc { - position: fixed; - right: 20px; - width: 300px; - padding-top: 20px; - overflow: scroll; - height: calc(100% - 3em - 20px); -} -#toc_header { - font-size: 1.8em; - font-weight: bold; -} -#toc > ul { - padding-left: 0; - list-style-type: none; -} -#toc > ul ul { padding-left: 20px; } -#toc > ul > li > a { display: none; } -img { max-width: 800px; } -") - -markdownToHTML( - file = markdown_fn, - output = output_fn, - stylesheet = custom_css, - options = c('toc', 'base64_images', 'highlight_code') -) diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index e7615f43..36a72c77 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -18,14 +18,17 @@ # Search each file using its regex for k, v in regexes.items(): - with open(v[0]) as x: - versions = x.read() - match = re.search(v[1], versions) - if match: - results[k] = "v{}".format(match.group(1)) + try: + with open(v[0]) as x: + versions = x.read() + match = re.search(v[1], versions) + if match: + results[k] = "v{}".format(match.group(1)) + except IOError: + results[k] = False # Remove software set to false in results -for k in results: +for k in list(results): if not results[k]: del(results[k]) diff --git a/conf/awsbatch.config b/conf/awsbatch.config deleted file mode 100644 index 14af5866..00000000 --- a/conf/awsbatch.config +++ /dev/null @@ -1,18 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running on AWS batch - * ------------------------------------------------- - * Base config needed for running with -profile awsbatch - */ -params { - config_profile_name = 'AWSBATCH' - config_profile_description = 'AWSBATCH Cloud Profile' - config_profile_contact = 'Alexander Peltzer (@apeltzer)' - config_profile_url = 'https://aws.amazon.com/de/batch/' -} - -aws.region = params.awsregion -process.executor = 'awsbatch' -process.queue = params.awsqueue -executor.awscli = '/home/ec2-user/miniconda/bin/aws' -params.tracedir = './' diff --git a/conf/base.config b/conf/base.config index 7e48056b..e208748f 100644 --- a/conf/base.config +++ b/conf/base.config @@ -13,22 +13,39 @@ process { // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } - time = { check_max( 2.h * task.attempt, 'time' ) } + memory = { check_max( 7.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } maxRetries = 1 maxErrors = '-1' // Process-specific resource requirements + // NOTE - Only one of the labels below are used in the fastqc process in the main script. + // If possible, it would be nice to keep the same label naming convention when + // adding in your processes. // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors -} - -params { - // Defaults only, expecting to be overwritten - max_memory = 128.GB - max_cpus = 16 - max_time = 240.h - igenomes_base = 's3://ngi-igenomes/igenomes/' + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 14.GB * task.attempt, 'memory' ) } + time = { check_max( 6.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 42.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 84.GB * task.attempt, 'memory' ) } + time = { check_max( 10.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withName:get_software_versions { + cache = false + } + } diff --git a/conf/igenomes.config b/conf/igenomes.config index d19e61f4..2de92422 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -9,139 +9,412 @@ params { // illumina iGenomes reference file paths - // TODO nf-core: Add new reference types and strip out those that are not needed genomes { 'GRCh37' { - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${baseDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" } 'GRCm38' { - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed" } 'TAIR10' { - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" } 'EB2' { - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" } 'UMD3.1' { - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" } 'WBcel235' { - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" } 'CanFam3.1' { - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" } 'GRCz10' { - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" } 'BDGP6' { - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" } 'EquCab2' { - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" } 'EB1' { - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" } 'Galgal4' { - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" } 'Gm01' { - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" } 'Mmul_1' { - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" } 'IRGSP-1.0' { - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" } 'CHIMP2.1.4' { - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" } 'Rnor_6.0' { - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" } 'R64-1-1' { - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" } 'EF2' { - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" } 'Sbi1' { - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" } 'Sscrofa10.2' { - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" } 'AGPv3' { - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${baseDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${baseDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" } } } diff --git a/conf/test.config b/conf/test.config index 17b6e41e..9ec602c0 100644 --- a/conf/test.config +++ b/conf/test.config @@ -4,20 +4,21 @@ * ------------------------------------------------- * Defines bundled input files and everything required * to run a fast and simple test. Use as follows: - * nextflow run nf-core/smrnaseq -profile test + * nextflow run nf-core/smrnaseq -profile test, */ params { config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on Travis + // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = 6.GB max_time = 48.h + // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - singleEnd = false + single_end = false readPaths = [ ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 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Most of the plots a ## Pipeline overview + The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: @@ -12,6 +13,7 @@ and processes data using the following steps: * [MultiQC](#multiqc) - aggregate report, describing results of the whole pipeline ## FastQC + [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your reads. It provides information about the quality score distribution across your reads, the per base sequence content (%T/A/G/C). You get information about adapter contamination and other overrepresented sequences. For further reading and documentation see the [FastQC help](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). @@ -25,8 +27,8 @@ For further reading and documentation see the [FastQC help](http://www.bioinform * `zips/sample_fastqc.zip` * zip file containing the FastQC report, tab-delimited data file and plot images - ## MultiQC + [MultiQC](http://multiqc.info) is a visualisation tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in within the report data directory. The pipeline has special steps which allow the software versions used to be reported in the MultiQC output for future traceability. diff --git a/docs/usage.md b/docs/usage.md index 505ff0ce..8884bc15 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -2,8 +2,6 @@ ## Table of contents - - * [Table of contents](#table-of-contents) * [Introduction](#introduction) * [Running the pipeline](#running-the-pipeline) @@ -12,20 +10,23 @@ * [Main arguments](#main-arguments) * [`-profile`](#-profile) * [`--reads`](#--reads) - * [`--singleEnd`](#--singleend) + * [`--single_end`](#--single_end) * [Reference genomes](#reference-genomes) * [`--genome` (using iGenomes)](#--genome-using-igenomes) * [`--fasta`](#--fasta) - * [`--igenomesIgnore`](#--igenomesignore) + * [`--igenomes_ignore`](#--igenomes_ignore) * [Job resources](#job-resources) * [Automatic resubmission](#automatic-resubmission) * [Custom resource requests](#custom-resource-requests) * [AWS Batch specific parameters](#aws-batch-specific-parameters) * [`--awsqueue`](#--awsqueue) * [`--awsregion`](#--awsregion) + * [`--awscli`](#--awscli) * [Other command line parameters](#other-command-line-parameters) * [`--outdir`](#--outdir) * [`--email`](#--email) + * [`--email_on_fail`](#--email_on_fail) + * [`--max_multiqc_email_size`](#--max_multiqc_email_size) * [`-name`](#-name) * [`-resume`](#-resume) * [`-c`](#-c) @@ -37,10 +38,9 @@ * [`--plaintext_email`](#--plaintext_email) * [`--monochrome_logs`](#--monochrome_logs) * [`--multiqc_config`](#--multiqc_config) - - ## Introduction + Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler. It is recommended to limit the Nextflow Java virtual machines memory. We recommend adding the following line to your environment (typically in `~/.bashrc` or `~./bash_profile`): @@ -52,6 +52,7 @@ NXF_OPTS='-Xms1g -Xmx4g' ## Running the pipeline + The typical command for running the pipeline is as follows: ```bash @@ -70,6 +71,7 @@ results # Finished results (configurable, see below) ``` ### Updating the pipeline + When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash @@ -77,31 +79,40 @@ nextflow pull nf-core/smrnaseq ``` ### Reproducibility + It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. First, go to the [nf-core/smrnaseq releases page](https://github.com/nf-core/smrnaseq/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. - ## Main arguments ### `-profile` -Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. Note that multiple profiles can be loaded, for example: `-profile docker` - the order of arguments is important! -If `-profile` is not specified at all the pipeline will be run locally and expects all software to be installed and available on the `PATH`. +Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. + +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Conda) - see below. + +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. + +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). + +Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! +They are loaded in sequence, so later profiles can overwrite earlier profiles. + +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. -* `awsbatch` - * A generic configuration profile to be used with AWS Batch. -* `conda` - * A generic configuration profile to be used with [conda](https://conda.io/docs/) - * Pulls most software from [Bioconda](https://bioconda.github.io/) * `docker` * A generic configuration profile to be used with [Docker](http://docker.com/) * Pulls software from dockerhub: [`nfcore/smrnaseq`](http://hub.docker.com/r/nfcore/smrnaseq/) * `singularity` * A generic configuration profile to be used with [Singularity](http://singularity.lbl.gov/) * Pulls software from DockerHub: [`nfcore/smrnaseq`](http://hub.docker.com/r/nfcore/smrnaseq/) +* `conda` + * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker or Singularity. + * A generic configuration profile to be used with [Conda](https://conda.io/docs/) + * Pulls most software from [Bioconda](https://bioconda.github.io/) * `test` * A profile with a complete configuration for automated testing * Includes links to test data so needs no other parameters @@ -109,6 +120,7 @@ If `-profile` is not specified at all the pipeline will be run locally and expec ### `--reads` + Use this to specify the location of your input FastQ files. For example: ```bash @@ -123,21 +135,22 @@ Please note the following requirements: If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz` -### `--singleEnd` -By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--singleEnd` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--reads`. For example: +### `--single_end` + +By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--reads`. For example: ```bash ---singleEnd --reads '*.fastq' +--single_end --reads '*.fastq' ``` It is not possible to run a mixture of single-end and paired-end files in one run. - ## Reference genomes The pipeline config files come bundled with paths to the illumina iGenomes reference index files. If running with docker or AWS, the configuration is set up to use the [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/) resource. ### `--genome` (using iGenomes) + There are 31 different species supported in the iGenomes references. To run the pipeline, you must specify which to use with the `--genome` flag. You can find the keys to specify the genomes in the [iGenomes config file](../conf/igenomes.config). Common genomes that are supported are: @@ -171,33 +184,48 @@ params { ``` + ### `--fasta` + If you prefer, you can specify the full path to your reference genome when you run the pipeline: ```bash --fasta '[path to Fasta reference]' ``` -### `--igenomesIgnore` +### `--igenomes_ignore` + Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`. ## Job resources + ### Automatic resubmission + Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. ### Custom resource requests + Wherever process-specific requirements are set in the pipeline, the default value can be changed by creating a custom config file. See the files hosted at [`nf-core/configs`](https://github.com/nf-core/configs/tree/master/conf) for examples. If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. -If you have any questions or issues please send us a message on [Slack](https://nf-core-invite.herokuapp.com/). +If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack). ## AWS Batch specific parameters -Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use the `-awsbatch` profile and then specify all of the following parameters. + +Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use [`-profile awsbatch`](https://github.com/nf-core/configs/blob/master/conf/awsbatch.config) and then specify all of the following parameters. + ### `--awsqueue` + The JobQueue that you intend to use on AWS Batch. + ### `--awsregion` -The AWS region to run your job in. Default is set to `eu-west-1` but can be adjusted to your needs. + +The AWS region in which to run your job. Default is set to `eu-west-1` but can be adjusted to your needs. + +### `--awscli` + +The [AWS CLI](https://www.nextflow.io/docs/latest/awscloud.html#aws-cli-installation) path in your custom AMI. Default: `/home/ec2-user/miniconda/bin/aws`. Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a S3 storage bucket of your choice - you'll get an error message notifying you if you didn't. @@ -206,12 +234,23 @@ Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a ### `--outdir` + The output directory where the results will be saved. ### `--email` + Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run. +### `--email_on_fail` + +This works exactly as with `--email`, except emails are only sent if the workflow is not successful. + +### `--max_multiqc_email_size` + +Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB). + ### `-name` + Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always). @@ -219,6 +258,7 @@ This is used in the MultiQC report (if not default) and in the summary HTML / e- **NB:** Single hyphen (core Nextflow option) ### `-resume` + Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. @@ -226,6 +266,7 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U **NB:** Single hyphen (core Nextflow option) ### `-c` + Specify the path to a specific config file (this is a core NextFlow command). **NB:** Single hyphen (core Nextflow option) @@ -233,7 +274,8 @@ Specify the path to a specific config file (this is a core NextFlow command). Note - you can use this to override pipeline defaults. ### `--custom_config_version` -Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default is set to `master`. + +Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`. ```bash ## Download and use config file with following git commid id @@ -241,6 +283,7 @@ Provide git commit id for custom Institutional configs hosted at `nf-core/config ``` ### `--custom_config_base` + If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the @@ -261,22 +304,28 @@ nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs > files + singularity containers + institutional configs in one go for you, to make this process easier. ### `--max_memory` + Use to set a top-limit for the default memory requirement for each process. Should be a string in the format integer-unit. eg. `--max_memory '8.GB'` ### `--max_time` + Use to set a top-limit for the default time requirement for each process. Should be a string in the format integer-unit. eg. `--max_time '2.h'` ### `--max_cpus` + Use to set a top-limit for the default CPU requirement for each process. Should be a string in the format integer-unit. eg. `--max_cpus 1` ### `--plaintext_email` + Set to receive plain-text e-mails instead of HTML formatted. ### `--monochrome_logs` + Set to disable colourful command line output and live life in monochrome. ### `--multiqc_config` + Specify a path to a custom MultiQC configuration file. diff --git a/environment.yml b/environment.yml index 130033b8..ad6c9f22 100644 --- a/environment.yml +++ b/environment.yml @@ -1,11 +1,15 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.0dev +name: nf-core-smrnaseq-1.0.0 channels: - conda-forge - bioconda - defaults dependencies: + - conda-forge::python=3.7.3 + - conda-forge::markdown=3.1.1 + - conda-forge::pymdown-extensions=6.0 + - conda-forge::pygments=2.5.2 # TODO nf-core: Add required software dependencies here - - fastqc=0.11.8 - - multiqc=1.7 + - bioconda::fastqc=0.11.8 + - bioconda::multiqc=1.7 diff --git a/main.nf b/main.nf index c384d621..230deb4b 100644 --- a/main.nf +++ b/main.nf @@ -9,7 +9,6 @@ ---------------------------------------------------------------------------------------- */ - def helpMessage() { // TODO nf-core: Add to this help message with new command line parameters log.info nfcoreHeader() @@ -22,39 +21,41 @@ def helpMessage() { nextflow run nf-core/smrnaseq --reads '*_R{1,2}.fastq.gz' -profile docker Mandatory arguments: - --reads Path to input data (must be surrounded with quotes) - -profile Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, awsbatch, test and more. + --reads [file] Path to input data (must be surrounded with quotes) + -profile [str] Configuration profile to use. Can use multiple (comma separated) + Available: conda, docker, singularity, test, awsbatch, and more Options: - --genome Name of iGenomes reference - --singleEnd Specifies that the input is single end reads + --genome [str] Name of iGenomes reference + --single_end [bool] Specifies that the input is single-end reads - References If not specified in the configuration file or you wish to overwrite any of the references. - --fasta Path to Fasta reference + References If not specified in the configuration file or you wish to overwrite any of the references + --fasta [file] Path to fasta reference Other options: - --outdir The output directory where the results will be saved - --email Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --maxMultiqcEmailFileSize Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. + --outdir [file] The output directory where the results will be saved + --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits + --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful + --max_multiqc_email_size [str] Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) + -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic AWSBatch options: - --awsqueue The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion The AWS Region for your AWS Batch job to run on + --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch + --awsregion [str] The AWS Region for your AWS Batch job to run on + --awscli [str] Path to the AWS CLI tool """.stripIndent() } -/* - * SET UP CONFIGURATION VARIABLES - */ - -// Show help emssage -if (params.help){ +// Show help message +if (params.help) { helpMessage() exit 0 } +/* + * SET UP CONFIGURATION VARIABLES + */ + // Check if genome exists in the config file if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" @@ -62,103 +63,101 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome // TODO nf-core: Add any reference files that are needed // Configurable reference genomes -fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false -if ( params.fasta ){ - fasta = file(params.fasta) - if( !fasta.exists() ) exit 1, "Fasta file not found: ${params.fasta}" -} // // NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// If you want to use the above in a process, define the following: +// If you want to use the channel below in a process, define the following: // input: -// file fasta from fasta +// file fasta from ch_fasta // - +params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false +if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } // Has the run name been specified by the user? // this has the bonus effect of catching both -name and --name custom_runName = params.name -if( !(workflow.runName ==~ /[a-z]+_[a-z]+/) ){ - custom_runName = workflow.runName +if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + custom_runName = workflow.runName } - -if( workflow.profile == 'awsbatch') { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (workflow.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." +if (workflow.profile.contains('awsbatch')) { + // AWSBatch sanity checking + if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." } // Stage config files -ch_multiqc_config = Channel.fromPath(params.multiqc_config) -ch_output_docs = Channel.fromPath("$baseDir/docs/output.md") +ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() +ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) /* * Create a channel for input read files */ -if(params.readPaths){ - if(params.singleEnd){ +if (params.readPaths) { + if (params.single_end) { Channel .from(params.readPaths) - .map { row -> [ row[0], [file(row[1][0])]] } + .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" } - .into { read_files_fastqc; read_files_trimming } + .into { ch_read_files_fastqc; ch_read_files_trimming } } else { Channel .from(params.readPaths) - .map { row -> [ row[0], [file(row[1][0]), file(row[1][1])]] } + .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" } - .into { read_files_fastqc; read_files_trimming } + .into { ch_read_files_fastqc; ch_read_files_trimming } } } else { Channel - .fromFilePairs( params.reads, size: params.singleEnd ? 1 : 2 ) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.reads}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --singleEnd on the command line." } - .into { read_files_fastqc; read_files_trimming } + .fromFilePairs(params.reads, size: params.single_end ? 1 : 2) + .ifEmpty { exit 1, "Cannot find any reads matching: ${params.reads}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } + .into { ch_read_files_fastqc; ch_read_files_trimming } } - // Header log info log.info nfcoreHeader() def summary = [:] -if(workflow.revision) summary['Pipeline Release'] = workflow.revision +if (workflow.revision) summary['Pipeline Release'] = workflow.revision summary['Run Name'] = custom_runName ?: workflow.runName // TODO nf-core: Report custom parameters here summary['Reads'] = params.reads summary['Fasta Ref'] = params.fasta -summary['Data Type'] = params.singleEnd ? 'Single-End' : 'Paired-End' +summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" -if(workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" +if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" summary['Output dir'] = params.outdir summary['Launch dir'] = workflow.launchDir summary['Working dir'] = workflow.workDir summary['Script dir'] = workflow.projectDir summary['User'] = workflow.userName -if(workflow.profile == 'awsbatch'){ - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue +if (workflow.profile.contains('awsbatch')) { + summary['AWS Region'] = params.awsregion + summary['AWS Queue'] = params.awsqueue + summary['AWS CLI'] = params.awscli } summary['Config Profile'] = workflow.profile -if(params.config_profile_description) summary['Config Description'] = params.config_profile_description -if(params.config_profile_contact) summary['Config Contact'] = params.config_profile_contact -if(params.config_profile_url) summary['Config URL'] = params.config_profile_url -if(params.email) { - summary['E-mail Address'] = params.email - summary['MultiQC maxsize'] = params.maxMultiqcEmailFileSize +if (params.config_profile_description) summary['Config Description'] = params.config_profile_description +if (params.config_profile_contact) summary['Config Contact'] = params.config_profile_contact +if (params.config_profile_url) summary['Config URL'] = params.config_profile_url +if (params.email || params.email_on_fail) { + summary['E-mail Address'] = params.email + summary['E-mail on failure'] = params.email_on_fail + summary['MultiQC maxsize'] = params.max_multiqc_email_size } log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") -log.info "\033[2m----------------------------------------------------\033[0m" +log.info "-\033[2m--------------------------------------------------\033[0m-" // Check the hostnames against configured profiles checkHostname() -def create_workflow_summary(summary) { - def yaml_file = workDir.resolve('workflow_summary_mqc.yaml') - yaml_file.text = """ +Channel.from(summary.collect{ [it.key, it.value] }) + .map { k,v -> "
$k
${v ?: 'N/A'}
" } + .reduce { a, b -> return [a, b].join("\n ") } + .map { x -> """ id: 'nf-core-smrnaseq-summary' description: " - this information is collected when the pipeline is started." section_name: 'nf-core/smrnaseq Workflow Summary' @@ -166,26 +165,23 @@ def create_workflow_summary(summary) { plot_type: 'html' data: |
-${summary.collect { k,v -> "
$k
${v ?: 'N/A'}
" }.join("\n")} + $x
- """.stripIndent() - - return yaml_file -} - + """.stripIndent() } + .set { ch_workflow_summary } /* * Parse software version numbers */ process get_software_versions { publishDir "${params.outdir}/pipeline_info", mode: 'copy', - saveAs: {filename -> - if (filename.indexOf(".csv") > 0) filename - else null - } + saveAs: { filename -> + if (filename.indexOf(".csv") > 0) filename + else null + } output: - file 'software_versions_mqc.yaml' into software_versions_yaml + file 'software_versions_mqc.yaml' into ch_software_versions_yaml file "software_versions.csv" script: @@ -199,30 +195,29 @@ process get_software_versions { """ } - - /* * STEP 1 - FastQC */ process fastqc { tag "$name" + label 'process_medium' publishDir "${params.outdir}/fastqc", mode: 'copy', - saveAs: {filename -> filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename"} + saveAs: { filename -> + filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" + } input: - set val(name), file(reads) from read_files_fastqc + set val(name), file(reads) from ch_read_files_fastqc output: - file "*_fastqc.{zip,html}" into fastqc_results + file "*_fastqc.{zip,html}" into ch_fastqc_results script: """ - fastqc -q $reads + fastqc --quiet --threads $task.cpus $reads """ } - - /* * STEP 2 - MultiQC */ @@ -230,28 +225,28 @@ process multiqc { publishDir "${params.outdir}/MultiQC", mode: 'copy' input: - file multiqc_config from ch_multiqc_config + file (multiqc_config) from ch_multiqc_config + file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) // TODO nf-core: Add in log files from your new processes for MultiQC to find! - file ('fastqc/*') from fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from software_versions_yaml.collect() - file workflow_summary from create_workflow_summary(summary) + file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) + file ('software_versions/*') from ch_software_versions_yaml.collect() + file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") output: - file "*multiqc_report.html" into multiqc_report + file "*multiqc_report.html" into ch_multiqc_report file "*_data" file "multiqc_plots" script: rtitle = custom_runName ? "--title \"$custom_runName\"" : '' rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' + custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ - multiqc -f $rtitle $rfilename --config $multiqc_config . + multiqc -f $rtitle $rfilename $custom_config_file . """ } - - /* * STEP 3 - Output Description HTML */ @@ -266,12 +261,10 @@ process output_documentation { script: """ - markdown_to_html.r $output_docs results_description.html + markdown_to_html.py $output_docs -o results_description.html """ } - - /* * Completion e-mail notification */ @@ -279,8 +272,8 @@ workflow.onComplete { // Set up the e-mail variables def subject = "[nf-core/smrnaseq] Successful: $workflow.runName" - if(!workflow.success){ - subject = "[nf-core/smrnaseq] FAILED: $workflow.runName" + if (!workflow.success) { + subject = "[nf-core/smrnaseq] FAILED: $workflow.runName" } def email_fields = [:] email_fields['version'] = workflow.manifest.version @@ -298,21 +291,20 @@ workflow.onComplete { email_fields['summary']['Date Completed'] = workflow.complete email_fields['summary']['Pipeline script file path'] = workflow.scriptFile email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if(workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if(workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if(workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - if(workflow.container) email_fields['summary']['Docker image'] = workflow.container + if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision email_fields['summary']['Nextflow Version'] = workflow.nextflow.version email_fields['summary']['Nextflow Build'] = workflow.nextflow.build email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - // TODO nf-core: If not using MultiQC, strip out this code (including params.maxMultiqcEmailFileSize) + // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) // On success try attach the multiqc report def mqc_report = null try { if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList){ + mqc_report = ch_multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList) { log.warn "[nf-core/smrnaseq] Found multiple reports from process 'multiqc', will use only one" mqc_report = mqc_report[0] } @@ -321,6 +313,12 @@ workflow.onComplete { log.warn "[nf-core/smrnaseq] Could not attach MultiQC report to summary email" } + // Check if we are only sending emails on failure + email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + // Render the TXT template def engine = new groovy.text.GStringTemplateEngine() def tf = new File("$baseDir/assets/email_template.txt") @@ -333,89 +331,89 @@ workflow.onComplete { def email_html = html_template.toString() // Render the sendmail template - def smail_fields = [ email: params.email, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.maxMultiqcEmailFileSize.toBytes() ] + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] def sf = new File("$baseDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() // Send the HTML e-mail - if (params.email) { + if (email_address) { try { - if( params.plaintext_email ){ throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[nf-core/smrnaseq] Sent summary e-mail to $params.email (sendmail)" + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "[nf-core/smrnaseq] Sent summary e-mail to $email_address (sendmail)" } catch (all) { - // Catch failures and try with plaintext - [ 'mail', '-s', subject, params.email ].execute() << email_txt - log.info "[nf-core/smrnaseq] Sent summary e-mail to $params.email (mail)" + // Catch failures and try with plaintext + [ 'mail', '-s', subject, email_address ].execute() << email_txt + log.info "[nf-core/smrnaseq] Sent summary e-mail to $email_address (mail)" } } // Write summary e-mail HTML to a file - def output_d = new File( "${params.outdir}/pipeline_info/" ) - if( !output_d.exists() ) { - output_d.mkdirs() + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() } - def output_hf = new File( output_d, "pipeline_report.html" ) + def output_hf = new File(output_d, "pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File( output_d, "pipeline_report.txt" ) + def output_tf = new File(output_d, "pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; c_red = params.monochrome_logs ? '' : "\033[0;31m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; - if (workflow.stats.ignoredCountFmt > 0 && workflow.success) { - log.info "${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}" - log.info "${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCountFmt} ${c_reset}" - log.info "${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCountFmt} ${c_reset}" + if (workflow.stats.ignoredCount > 0 && workflow.success) { + log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" + log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" + log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" } - if(workflow.success){ - log.info "${c_purple}[nf-core/smrnaseq]${c_green} Pipeline completed successfully${c_reset}" + if (workflow.success) { + log.info "-${c_purple}[nf-core/smrnaseq]${c_green} Pipeline completed successfully${c_reset}-" } else { checkHostname() - log.info "${c_purple}[nf-core/smrnaseq]${c_red} Pipeline completed with errors${c_reset}" + log.info "-${c_purple}[nf-core/smrnaseq]${c_red} Pipeline completed with errors${c_reset}-" } } -def nfcoreHeader(){ +def nfcoreHeader() { // Log colors ANSI codes - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; + c_dim = params.monochrome_logs ? '' : "\033[2m"; + c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; c_white = params.monochrome_logs ? '' : "\033[0;37m"; + c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - return """ ${c_dim}----------------------------------------------------${c_reset} + return """ -${c_dim}--------------------------------------------------${c_reset}- ${c_green},--.${c_black}/${c_green},-.${c_reset} ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} ${c_green}`._,._,\'${c_reset} ${c_purple} nf-core/smrnaseq v${workflow.manifest.version}${c_reset} - ${c_dim}----------------------------------------------------${c_reset} + -${c_dim}--------------------------------------------------${c_reset}- """.stripIndent() } -def checkHostname(){ +def checkHostname() { def c_reset = params.monochrome_logs ? '' : "\033[0m" def c_white = params.monochrome_logs ? '' : "\033[0;37m" def c_red = params.monochrome_logs ? '' : "\033[1;91m" def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if(params.hostnames){ + if (params.hostnames) { def hostname = "hostname".execute().text.trim() params.hostnames.each { prof, hnames -> hnames.each { hname -> - if(hostname.contains(hname) && !workflow.profile.contains(prof)){ + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { log.error "====================================================\n" + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + diff --git a/nextflow.config b/nextflow.config index 6fef9df8..c2aa9d2f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -10,29 +10,35 @@ params { // Workflow flags // TODO nf-core: Specify your pipeline's command line flags + genome = false reads = "data/*{1,2}.fastq.gz" - singleEnd = false + single_end = false outdir = './results' // Boilerplate options name = false - multiqc_config = "$baseDir/assets/multiqc_config.yaml" + multiqc_config = false email = false - maxMultiqcEmailFileSize = 25.MB + email_on_fail = false + max_multiqc_email_size = 25.MB plaintext_email = false monochrome_logs = false help = false - igenomes_base = "./iGenomes" + igenomes_base = 's3://ngi-igenomes/igenomes/' tracedir = "${params.outdir}/pipeline_info" - awsqueue = false - awsregion = 'eu-west-1' - igenomesIgnore = false + igenomes_ignore = false custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false config_profile_description = false config_profile_contact = false config_profile_url = false + + // Defaults only, expecting to be overwritten + max_memory = 128.GB + max_cpus = 16 + max_time = 240.h + } // Container slug. Stable releases should specify release tag! @@ -50,19 +56,33 @@ try { } profiles { - awsbatch { includeConfig 'conf/awsbatch.config' } conda { process.conda = "$baseDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } - docker { docker.enabled = true } - singularity { singularity.enabled = true } + docker { + docker.enabled = true + // Avoid this error: + // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. + // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 + // once this is established and works well, nextflow might implement this behavior as new default. + docker.runOptions = '-u \$(id -u):\$(id -g)' + } + singularity { + singularity.enabled = true + singularity.autoMounts = true + } test { includeConfig 'conf/test.config' } } // Load igenomes.config if required -if(!params.igenomesIgnore){ +if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' } +// Export this variable to prevent local Python libraries from conflicting with those in the container +env { + PYTHONNOUSERSITE = 1 +} + // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] @@ -89,16 +109,16 @@ manifest { homePage = 'https://github.com/nf-core/smrnaseq' description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' - nextflowVersion = '>=0.32.0' - version = '1.0dev' + nextflowVersion = '>=19.10.0' + version = '1.0.0' } // Function to ensure that resource requirements don't go beyond // a maximum limit def check_max(obj, type) { - if(type == 'memory'){ + if (type == 'memory') { try { - if(obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) return params.max_memory as nextflow.util.MemoryUnit else return obj @@ -106,9 +126,9 @@ def check_max(obj, type) { println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" return obj } - } else if(type == 'time'){ + } else if (type == 'time') { try { - if(obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) return params.max_time as nextflow.util.Duration else return obj @@ -116,7 +136,7 @@ def check_max(obj, type) { println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" return obj } - } else if(type == 'cpus'){ + } else if (type == 'cpus') { try { return Math.min( obj, params.max_cpus as int ) } catch (all) { From 949b5c4ea21f45463ebfb6ebe9ee1a9d96b3f6d8 Mon Sep 17 00:00:00 2001 From: Miles Date: Mon, 2 Mar 2020 11:30:32 +1300 Subject: [PATCH 014/146] custom protocol implementation A quick patch to fix the handling of --protocol (specifically custom) options when passing off to miRtrace. --- main.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index f400b181..a344a2b4 100644 --- a/main.nf +++ b/main.nf @@ -711,6 +711,7 @@ process mirtrace { script: primer = (protocol=="cats") ? " " : " --adapter $three_prime_adapter " + protocol_opt = (protocol=="custom") ? " " : " --protocol $protocol " """ for i in $reads do @@ -722,7 +723,7 @@ process mirtrace { mirtrace qc \\ --species $params.mirtrace_species \\ $primer \\ - --protocol $protocol \\ + $protocol_opt $protocol \\ --config mirtrace_config \\ --write-fasta \\ --output-dir mirtrace \\ From 675b45c093a4b4b697b9eee3410cf3fde8837858 Mon Sep 17 00:00:00 2001 From: Miles Date: Mon, 2 Mar 2020 11:49:30 +1300 Subject: [PATCH 015/146] improved --protocol documentation Amended documentation around `--protocol` to better reflect that the user can use a custom protocol and provide their own adapter and trimming parameters. --- docs/usage.md | 15 +++++++++------ 1 file changed, 9 insertions(+), 6 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 384bcd90..9b5b5747 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -139,12 +139,15 @@ Default: "illumina" --protocol [one protocol listed in the table below] ``` -| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence | -| :------------ | :-------------------------------------- | :----------------------------------- | :------------------- | -| illumina | Illumina TruSeq Small RNA | clip_R1 = 0; three_prime_clip_R1 = 0 | TGGAATTCTCGGGTGCCAAGG | -| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | clip_R1 = 4; three_prime_clip_R1 = 4 | TGGAATTCTCGGGTGCCAAGG | -| qiaseq | QIAGEN QIAseq miRNA | clip_R1 = 0; three_prime_clip_R1 = 0 | AACTGTAGGCACCATCAAT | -| cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | GATCGGAAGAGCACACGTCTG | +| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence | +| :----------------- | :-------------------------------------- | :----------------------------------- | :------------------- | +| illumina | Illumina TruSeq Small RNA | clip_R1 = 0; three_prime_clip_R1 = 0 | TGGAATTCTCGGGTGCCAAGG | +| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | clip_R1 = 4; three_prime_clip_R1 = 4 | TGGAATTCTCGGGTGCCAAGG | +| qiaseq | QIAGEN QIAseq miRNA | clip_R1 = 0; three_prime_clip_R1 = 0 | AACTGTAGGCACCATCAAT | +| cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | GATCGGAAGAGCACACGTCTG | +| custom* | user defined | user defined | user defined | + +***Note:** when running `--protocol custom` the user must define the 3' Adapter Sequence. If trimming parameters aren't provided the pipeline will deafult to clip_R1 = 0 and three_prime_clip_R1 = 0 (i.e. no extra clipping). ## Reference genomes From 2160233f0c8edb23405491c91528f0fef6d5830c Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 31 Mar 2020 17:48:16 -0400 Subject: [PATCH 016/146] update docker with new packages to migrate to new template I am updating with the new TEMPLATE and some packages are missing. --- environment.yml | 46 +++++++++++++++++++++++++--------------------- 1 file changed, 25 insertions(+), 21 deletions(-) diff --git a/environment.yml b/environment.yml index 753f672b..ed5330dd 100644 --- a/environment.yml +++ b/environment.yml @@ -1,32 +1,36 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.0.0 +name: nf-core-smrnaseq-1.0.1dev channels: - conda-forge - bioconda - defaults dependencies: ## conda-forge packages - - conda-forge::r-base=3.6.1 + - conda-forge::r-base=3.6.3 - conda-forge::openjdk=11.0.1 # Needed for FastQC - conda build hangs without this - - conda-forge::r-statmod=1.4.32 - - conda-forge::r-data.table=1.12.2 - - conda-forge::r-gplots=3.0.1.1 - - conda-forge::r-r.methodss3=1.7.1 - - conda-forge::r-markdown=1.0 - - conda-forge::matplotlib=3.0.3 # Current 3.1.0 build incompatible with multiqc=1.7 + - conda-forge::r-statmod=1.4.34 + - conda-forge::r-data.table=1.12.8 + - conda-forge::r-gplots=3.0.3 + - conda-forge::r-r.methodss3=1.8.0 +# - conda-forge::r-markdown=1.0 +# - conda-forge::matplotlib=3.0.3 # Current 3.1.0 build incompatible with multiqc=1.7 + - conda-forge::python=3.8.2 + - conda-forge::markdown=3.2.1 + - conda-forge::pymdown-extensions=6.0 + - conda-forge::pygments=2.6.1 ## bioconda packages - - fastqc=0.11.8 - - trim-galore=0.6.3 - - samtools=1.9 - - bowtie=1.2.2 - - multiqc=1.7 - - mirtop=0.4.22 - - seqcluster=1.2.5 - - htseq=0.11.2 - - fastx_toolkit=0.0.14 - - seqkit=0.10.1 - - mirtrace=1.0.0 - - bioconductor-edger=3.26.5 - - bioconductor-limma=3.40.2 \ No newline at end of file + - bioconda::fastqc=0.11.9 + - bioconda::trim-galore=0.6.5 + - bioconda::samtools=1.10 + - bioconda::bowtie=1.2.3 + - bioconda::multiqc=1.8 + - bioconda::mirtop=0.4.23 + - bioconda::seqcluster=1.2.7 + - bioconda::htseq=0.11.3 + - bioconda::fastx_toolkit=0.0.14 + - bioconda::seqkit=0.12.0 + - bioconda::mirtrace=1.0.1 + - bioconda::bioconductor-edger=3.28.0 + - bioconda::bioconductor-limma=3.42.0 From a03cd1f066599b90a3be34460d5efd01cc4a7311 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 31 Mar 2020 17:58:03 -0400 Subject: [PATCH 017/146] update changelog --- CHANGELOG.md | 31 +++++++++++++++++++++++++++++++ 1 file changed, 31 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ac0e756c..c618b5ac 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,5 +1,36 @@ # nf-core/smrnaseq: Changelog +## v1.0.1dev - 2020-03-31 + +* Update conda environment with new packages and updates + +#### Dependency Updates + + * conda-forge::r-base=3.6.1 -> 3.6.3 + * conda-forge::r-statmod=1.4.32 -> 1.4.34 + * conda-forge::r-data.table=1.12.2 -> 1.12.8 + * conda-forge::r-gplots=3.0.1.1 -> 3.0.3 + * conda-forge::r-r.methodss3=1.7.1 -> 1.8.0 + * fastqc=0.11.8 -> 0.11.9 + * trim-galore=0.6.3 -> 0.6.5 + * samtools=1.9 -> 1.10 + * bowtie=1.2.2 -> 1.2.3 + * multiqc=1.7 -> 1.8 + * mirtop=0.4.22 -> 0.4.23 + * seqcluster=1.2.5 -> 1.2.7 + * htseq=0.11.2 -> 0.11.3 + * fastx_toolkit=0.0.14 -> 0.0.14 + * seqkit=0.10.1 -> 0.12.0 + * mirtrace=1.0.0 -> 1.0.1 + * bioconductor-edger=3.26.5 -> 3.28.0 + * bioconductor-limma=3.40.2 -> 3.42.0 + * Added conda-forge::python=3.8.2 + * Added conda-forge::markdown=3.2.1 + * Added conda-forge::pymdown-extensions=6.0 + * Added conda-forge::pygments=2.6.1 + * Removed conda-forge::r-markdown=1.0 + * Removed conda-forge::matplotlib=3.0.3 + ## v1.0.0 - 2019-09-19 * Add figures to output documentation * Add samtools stats for genome alignments From 7f8bc88d411010d318f54c01c14e9660ca044da7 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Thu, 2 Apr 2020 19:45:30 -0400 Subject: [PATCH 018/146] update Docker env path --- CHANGELOG.md | 4 ++-- Dockerfile | 14 ++++++++++---- environment.yml | 2 +- 3 files changed, 13 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index c618b5ac..1d7c5254 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,10 +1,10 @@ # nf-core/smrnaseq: Changelog -## v1.0.1dev - 2020-03-31 +## v1.0.1dev * Update conda environment with new packages and updates -#### Dependency Updates +#### Packaged software updates * conda-forge::r-base=3.6.1 -> 3.6.3 * conda-forge::r-statmod=1.4.32 -> 1.4.34 diff --git a/Dockerfile b/Dockerfile index 66b720e1..77c5e392 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,7 +1,13 @@ -FROM nfcore/base -LABEL authors="Alexander Peltzer " \ - description="Docker image containing all requirements for nf-core/smrnaseq pipeline" +FROM nfcore/base:1.9 +LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ + description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" +# Install the conda environment COPY environment.yml / RUN conda env create -f /environment.yml && conda clean -a -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.0/bin:$PATH + +# Add conda installation dir to PATH (instead of doing 'conda activate') +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.1dev/bin:$PATH + +# Dump the details of the installed packages to a file for posterity +RUN conda env export --name nf-core-smrnaseq-1.0.1dev > nf-core-smrnaseq-1.0.1dev.yml diff --git a/environment.yml b/environment.yml index ed5330dd..e944b254 100644 --- a/environment.yml +++ b/environment.yml @@ -33,4 +33,4 @@ dependencies: - bioconda::seqkit=0.12.0 - bioconda::mirtrace=1.0.1 - bioconda::bioconductor-edger=3.28.0 - - bioconda::bioconductor-limma=3.42.0 + - bioconda::bioconductor-limma=3.42.0 \ No newline at end of file From 4f131ff84a89836da26428ee145689bf2557bee7 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Fri, 3 Apr 2020 13:41:30 -0400 Subject: [PATCH 019/146] get docker working --- environment.yml | 28 ++++++++++++++-------------- 1 file changed, 14 insertions(+), 14 deletions(-) diff --git a/environment.yml b/environment.yml index e944b254..c981d758 100644 --- a/environment.yml +++ b/environment.yml @@ -7,23 +7,23 @@ channels: - defaults dependencies: ## conda-forge packages - - conda-forge::r-base=3.6.3 + - conda-forge::r-base=3.6.1 - conda-forge::openjdk=11.0.1 # Needed for FastQC - conda build hangs without this - - conda-forge::r-statmod=1.4.34 - - conda-forge::r-data.table=1.12.8 - - conda-forge::r-gplots=3.0.3 - - conda-forge::r-r.methodss3=1.8.0 -# - conda-forge::r-markdown=1.0 -# - conda-forge::matplotlib=3.0.3 # Current 3.1.0 build incompatible with multiqc=1.7 - - conda-forge::python=3.8.2 - - conda-forge::markdown=3.2.1 + - conda-forge::r-statmod=1.4.32 + - conda-forge::r-data.table=1.12.4 + - conda-forge::r-gplots=3.0.1.1 + - conda-forge::r-r.methodss3=1.7.1 + # - conda-forge::r-markdown=1.0 + - conda-forge::matplotlib=3.0.3 # Current 3.1.0 build incompatible with multiqc=1.7 + - conda-forge::python=3.7.3 + - conda-forge::markdown=3.1.1 - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.6.1 + - conda-forge::pygments=2.5.2 - ## bioconda packages + # bioconda packages - bioconda::fastqc=0.11.9 - bioconda::trim-galore=0.6.5 - - bioconda::samtools=1.10 + - bioconda::samtools=1.9 - bioconda::bowtie=1.2.3 - bioconda::multiqc=1.8 - bioconda::mirtop=0.4.23 @@ -32,5 +32,5 @@ dependencies: - bioconda::fastx_toolkit=0.0.14 - bioconda::seqkit=0.12.0 - bioconda::mirtrace=1.0.1 - - bioconda::bioconductor-edger=3.28.0 - - bioconda::bioconductor-limma=3.42.0 \ No newline at end of file + - bioconda::bioconductor-edger=3.26.5 + - bioconda::bioconductor-limma=3.40.2 \ No newline at end of file From 6bbfe68028453fd195ef2347b26d78f3f362aec2 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Fri, 3 Apr 2020 13:51:41 -0400 Subject: [PATCH 020/146] Update changelog --- CHANGELOG.md | 15 +++------------ environment.yml | 3 +-- 2 files changed, 4 insertions(+), 14 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 1d7c5254..53ee5725 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,14 +6,8 @@ #### Packaged software updates - * conda-forge::r-base=3.6.1 -> 3.6.3 - * conda-forge::r-statmod=1.4.32 -> 1.4.34 - * conda-forge::r-data.table=1.12.2 -> 1.12.8 - * conda-forge::r-gplots=3.0.1.1 -> 3.0.3 - * conda-forge::r-r.methodss3=1.7.1 -> 1.8.0 * fastqc=0.11.8 -> 0.11.9 * trim-galore=0.6.3 -> 0.6.5 - * samtools=1.9 -> 1.10 * bowtie=1.2.2 -> 1.2.3 * multiqc=1.7 -> 1.8 * mirtop=0.4.22 -> 0.4.23 @@ -22,14 +16,11 @@ * fastx_toolkit=0.0.14 -> 0.0.14 * seqkit=0.10.1 -> 0.12.0 * mirtrace=1.0.0 -> 1.0.1 - * bioconductor-edger=3.26.5 -> 3.28.0 - * bioconductor-limma=3.40.2 -> 3.42.0 - * Added conda-forge::python=3.8.2 - * Added conda-forge::markdown=3.2.1 + * Added conda-forge::python=3.7.3 + * Added conda-forge::markdown=3.1.1 * Added conda-forge::pymdown-extensions=6.0 - * Added conda-forge::pygments=2.6.1 + * Added conda-forge::pygments=2.5.2 * Removed conda-forge::r-markdown=1.0 - * Removed conda-forge::matplotlib=3.0.3 ## v1.0.0 - 2019-09-19 * Add figures to output documentation diff --git a/environment.yml b/environment.yml index c981d758..4274069f 100644 --- a/environment.yml +++ b/environment.yml @@ -13,8 +13,7 @@ dependencies: - conda-forge::r-data.table=1.12.4 - conda-forge::r-gplots=3.0.1.1 - conda-forge::r-r.methodss3=1.7.1 - # - conda-forge::r-markdown=1.0 - - conda-forge::matplotlib=3.0.3 # Current 3.1.0 build incompatible with multiqc=1.7 + - conda-forge::matplotlib=3.0.3 # To avoid conflics with multiple packages during docker build - conda-forge::python=3.7.3 - conda-forge::markdown=3.1.1 - conda-forge::pymdown-extensions=6.0 From 07611c3c69d8db40d5e8f7870810f10a78fc0652 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 14 Apr 2020 18:04:51 -0400 Subject: [PATCH 021/146] update change log --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 231e4f2d..860af29d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -4,6 +4,7 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0. ## v1.0.1dev +* Update with the latest TEMPLATE version and nf-core 1.9 * Update conda environment with new packages and updates #### Packaged software updates From c510dc89b676e4f3ffce29930d74626aff856945 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Tue, 14 Apr 2020 18:16:09 -0400 Subject: [PATCH 022/146] fix markdown linting errors --- CHANGELOG.md | 39 ++++++++++++++++++++++----------------- README.md | 15 +++++---------- docs/output.md | 8 ++++++-- docs/usage.md | 24 +++++++++++++++++++++--- 4 files changed, 54 insertions(+), 32 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 860af29d..cd74f84e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,4 +1,5 @@ # nf-core/smrnaseq: Changelog + The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html). @@ -7,25 +8,26 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0. * Update with the latest TEMPLATE version and nf-core 1.9 * Update conda environment with new packages and updates -#### Packaged software updates +### Packaged software updates - * fastqc=0.11.8 -> 0.11.9 - * trim-galore=0.6.3 -> 0.6.5 - * bowtie=1.2.2 -> 1.2.3 - * multiqc=1.7 -> 1.8 - * mirtop=0.4.22 -> 0.4.23 - * seqcluster=1.2.5 -> 1.2.7 - * htseq=0.11.2 -> 0.11.3 - * fastx_toolkit=0.0.14 -> 0.0.14 - * seqkit=0.10.1 -> 0.12.0 - * mirtrace=1.0.0 -> 1.0.1 - * Added conda-forge::python=3.7.3 - * Added conda-forge::markdown=3.1.1 - * Added conda-forge::pymdown-extensions=6.0 - * Added conda-forge::pygments=2.5.2 - * Removed conda-forge::r-markdown=1.0 +* fastqc=0.11.8 -> 0.11.9 +* trim-galore=0.6.3 -> 0.6.5 +* bowtie=1.2.2 -> 1.2.3 +* multiqc=1.7 -> 1.8 +* mirtop=0.4.22 -> 0.4.23 +* seqcluster=1.2.5 -> 1.2.7 +* htseq=0.11.2 -> 0.11.3 +* fastx_toolkit=0.0.14 -> 0.0.14 +* seqkit=0.10.1 -> 0.12.0 +* mirtrace=1.0.0 -> 1.0.1 +* Added conda-forge::python=3.7.3 +* Added conda-forge::markdown=3.1.1 +* Added conda-forge::pymdown-extensions=6.0 +* Added conda-forge::pygments=2.5.2 +* Removed conda-forge::r-markdown=1.0 ## v1.0.0 - 2019-09-19 + * Add figures to output documentation * Add samtools stats for genome alignments * Add seqkit and remove razers @@ -33,7 +35,8 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0. * Adapt code and docs to [nf-core](http://nf-co.re/) template * Update tools and Dockerfile/Singularity to match current template -#### Dependency Updates +### Dependency Updates + * openjdk 8.0.144 -> 11.0.1 * fastqc 0.11.7 -> 0.11.8 * trim-galore 0.5.0 -> 0.6.2 @@ -57,11 +60,13 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0. * bioconductor-limma=3.40.0 -> 3.40.2 ## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2019-01-10 + * Add "protocol" with pre-defined settings * Add miRTrace in the pipeline. * Software updates: multiqc 1.6 to 1.7. ## [1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0) - 2018-08-06 + * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. * Add option for sequencing centre in BAM file. diff --git a/README.md b/README.md index a889d158..530d4e83 100644 --- a/README.md +++ b/README.md @@ -9,14 +9,13 @@ [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/smrnaseq.svg)](https://hub.docker.com/r/nfcore/smrnaseq) -<<<<<<< HEAD [![DOI](https://zenodo.org/badge/140590861.svg)](https://zenodo.org/badge/latestdoi/140590861) -======= ## Introduction +**nf-core/smrnaseq** is a bioinformatics best-practice analysis pipeline used for small RNA sequencing data. + The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. ->>>>>>> TEMPLATE ## Quick Start @@ -40,14 +39,8 @@ nextflow run nf-core/smrnaseq -profile --re See [usage docs](docs/usage.md) for all of the available options when running the pipeline. -## Introduction -**nf-core/smrnaseq** is a bioinformatics best-practice analysis pipeline used for small RNA sequencing data. - -The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. - ### Pipeline summary - 1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/)) 1. Insert Size calculation @@ -68,8 +61,8 @@ The pipeline is built using Nextflow, a workflow tool to run tasks across multip 7. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace)) 8. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/)) - ### Documentation + The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found in the `docs/` directory: 1. [Installation](https://nf-co.re/usage/installation) @@ -82,6 +75,7 @@ The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found 5. [Troubleshooting](https://nf-co.re/usage/troubleshooting) ## Credits + nf-core/smrnaseq was originally written for use at the [National Genomics Infrastructure](https://portal.scilifelab.se/genomics/) at [SciLifeLab](http://www.scilifelab.se/) in Stockholm, Sweden, by Phil Ewels (@ewels), Chuan Wang (@chuan-wang) and Rickard Hammarén (@Hammarn). Updated by Lorena Pantano (@lpantano) from MIT. ## Contributions and Support @@ -91,6 +85,7 @@ If you would like to contribute to this pipeline, please see the [contributing g For further information or help, don't hesitate to get in touch on [Slack](https://nfcore.slack.com/channels/smrnaseq) (you can join with [this invite](https://nf-co.re/join/slack)). ## Citation + You can cite the `nf-core` publication as follows: > **The nf-core framework for community-curated bioinformatics pipelines.** diff --git a/docs/output.md b/docs/output.md index ee63c3b5..b293f931 100644 --- a/docs/output.md +++ b/docs/output.md @@ -57,6 +57,7 @@ This is an example of the output we can get: ![cutadapt](images/cutadapt_plot.png) ## Bowtie - miRNAs + [Bowtie](http://bowtie-bio.sourceforge.net/index.shtml) is used for mapping adapter trimmed reads against the mature miRNAs and miRNA precursors (hairpins) in [miRBase](http://www.mirbase.org/). **Output directory: `results/bowtie`** @@ -71,6 +72,7 @@ This is an example of the output we can get: * Unmapped reads against miRNA precursors (hairpins) ## SAMtools + [SAMtools](http://samtools.sourceforge.net/) is used for sorting and indexing the output BAM files from Bowtie. In addition, the numbers of features are counted with the `idxstats` option. **Output directory: `results/bowtie`** @@ -91,6 +93,7 @@ This is an example of the output we can get: ![samtools](images/samtools_alignment_plot.png) ## edgeR + [edgeR](https://bioconductor.org/packages/release/bioc/html/edgeR.html) is an R package used for differential expression analysis of RNA-seq expression profiles. **Output directory: `results/edgeR`** @@ -109,11 +112,11 @@ This is an example of the output we can get: **Example**: MDS plot of 10 samples based on their expression profiles of mature miRNAs. Here we can see that samples cluster based on different sample types and library preparation kits. ![edgeR](images/Example_MDS_plot.png) - **Example**: Heatmap of tumor and normal samples based on the top differentially expressed mature miRNAs. ![edgeR](images/Example_heatmap.png) ## Bowtie - QC + [Bowtie](http://bowtie-bio.sourceforge.net/index.shtml) is used for mapping adapter trimmed reads against the reference genome for quality control purposes. **Output directory: `results/bowtie_ref`** @@ -124,6 +127,7 @@ This is an example of the output we can get: * Raw mapped read counts and stats of mature miRNAs ## mirtop + [mirtop](https://github.com/miRTop/mirtop) is used to parse the BAM files from `bowtie` alignment, and produce a [mirgff3](https://github.com/miRTop/mirGFF3) file with information about miRNAs and isomirs. ** Output directory: `results/mirtop` ** @@ -134,6 +138,7 @@ This is an example of the output we can get: * `mirna.tsv`: tabular file with miRNA counts after summarizing unique isomiRs for each miRNA ## miRTrace + [miRTrace](https://github.com/friedlanderlab/mirtrace) is a quality control specifically for small RNA sequencing data (smRNA-Seq). Each sample is characterized by profiling sequencing quality, read length, sequencing depth and miRNA complexity and also the amounts of miRNAs versus undesirable sequences (derived from tRNAs, rRNAs and sequencing artifacts). **Output directory: `results/miRTrace`** @@ -153,7 +158,6 @@ Refer to the [tool manual](https://github.com/friedlanderlab/mirtrace/blob/maste ![mirtrace](images/mirtrace_plot.png) - ## MultiQC [MultiQC](http://multiqc.info) is a visualisation tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in within the report data directory. diff --git a/docs/usage.md b/docs/usage.md index 357e7272..5cf4941f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -147,8 +147,8 @@ Please note the following requirements: 1. The path must be enclosed in quotes 2. The path must have at least one `*` wildcard character - ### `--protocol` + Protocol for constructing smRNA-seq libraries. Note that trimming parameters and 3' adapter sequence are pre-defined with a specified protocol. Default: "illumina" @@ -163,6 +163,7 @@ Default: "illumina" | qiaseq | QIAGEN QIAseq miRNA | clip_R1 = 0; three_prime_clip_R1 = 0 | AACTGTAGGCACCATCAAT | | cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | AAAAAAAAAAA + GATCGGAAGAGCACACGTCTG (only polyA is used for trimming) | ======= + ### `--single_end` This pipeline is not compatible with paired-data @@ -210,6 +211,7 @@ params { ``` ### `--saveReference` + Supply this parameter to save any generated reference genome files to your results folder. These can then be used for future pipeline runs, reducing processing times. ### `--fasta` @@ -225,6 +227,7 @@ If you prefer, you can specify the full path to your reference genome when you r Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`. ### `--mature` + If you prefer, you can specify the full path to the FASTA file of mature miRNAs when you run the pipeline: ```bash @@ -232,6 +235,7 @@ If you prefer, you can specify the full path to the FASTA file of mature miRNAs ``` ### `--hairpin` + If you prefer, you can specify the full path to the FASTA file of miRNA precursors when you run the pipeline: ```bash @@ -239,6 +243,7 @@ If you prefer, you can specify the full path to the FASTA file of miRNA precurso ``` ### `--bt_index` + If you prefer, you can specify the full path to your reference genome when you run the pipeline: ```bash @@ -246,25 +251,36 @@ If you prefer, you can specify the full path to your reference genome when you r ``` ## Trimming options + ### `--min_length [int]` + Discard reads that became shorter than length [int] because of either quality or adapter trimming. Default: 18 + ### `--clip_R1 [int]` + Instructs Trim Galore to remove bp from the 5' end of read 1 + ### `--three_prime_clip_R1 [int]` + Instructs Trim Galore to remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed + ### `--three_prime_adapter [sequence]` + Instructs Trim Galore to remove 3' adapters which are typically used in smRNA-seq library preparation ## Skipping QC steps + ### `--skipQC` + Skip all QC steps aside from MultiQC ### `--skipFastqc` + Skip FastQC ### `--skipMultiqc` -Skip MultiQC +Skip MultiQC ## Job resources @@ -299,6 +315,7 @@ The [AWS CLI](https://www.nextflow.io/docs/latest/awscloud.html#aws-cli-installa Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a S3 storage bucket of your choice - you'll get an error message notifying you if you didn't. ## Other command line parameters + ### `--outdir` The output directory where the results will be saved. @@ -324,6 +341,7 @@ This is used in the MultiQC report (if not default) and in the summary HTML / e- **NB:** Single hyphen (core Nextflow option) ### `--seq_center` + Text about sequencing center which will be added in the header of output bam files. ### `-resume` @@ -348,12 +366,12 @@ process.$multiqc.module = [] ``` ## Stand-alone scripts + The `bin` directory contains some scripts used by the pipeline which may also be run manually: * `edgeR_miRBase.r` * R script using for processing reads counts of mature miRNAs and miRNA precursors (hairpins). - ### `--custom_config_version` Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`. From e0e047146599184c40b0343439c4901aa71f7cc6 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 30 Jul 2020 20:10:46 +0000 Subject: [PATCH 023/146] Template update for nf-core/tools version 1.10.1 --- .github/.dockstore.yml | 5 + .github/CONTRIBUTING.md | 2 +- .github/ISSUE_TEMPLATE/bug_report.md | 15 +- .github/ISSUE_TEMPLATE/feature_request.md | 12 +- .github/PULL_REQUEST_TEMPLATE.md | 15 +- .github/workflows/awsfulltest.yml | 40 ++++ .github/workflows/awstest.yml | 40 ++++ .github/workflows/branch.yml | 30 ++- .github/workflows/ci.yml | 47 +++- .github/workflows/linting.yml | 13 +- .github/workflows/push_dockerhub.yml | 39 ++++ .gitignore | 1 + CHANGELOG.md | 8 +- CODE_OF_CONDUCT.md | 6 +- Dockerfile | 12 +- README.md | 42 ++-- bin/markdown_to_html.py | 43 ++-- bin/scrape_software_versions.py | 36 +-- conf/igenomes.config | 1 + conf/test.config | 2 +- conf/test_full.config | 22 ++ docs/README.md | 20 +- docs/output.md | 50 ++-- docs/usage.md | 272 +++------------------- environment.yml | 2 +- main.nf | 53 +++-- nextflow.config | 9 +- nextflow_schema.json | 259 ++++++++++++++++++++ 28 files changed, 685 insertions(+), 411 deletions(-) create mode 100644 .github/.dockstore.yml create mode 100644 .github/workflows/awsfulltest.yml create mode 100644 .github/workflows/awstest.yml create mode 100644 .github/workflows/push_dockerhub.yml create mode 100644 conf/test_full.config create mode 100644 nextflow_schema.json diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml new file mode 100644 index 00000000..030138a0 --- /dev/null +++ b/.github/.dockstore.yml @@ -0,0 +1,5 @@ +# Dockstore config version, not pipeline version +version: 1.2 +workflows: + - subclass: nfl + primaryDescriptorPath: /nextflow.config diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 0562bf83..bdf8a75d 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -54,4 +54,4 @@ These tests are run both with the latest available version of `Nextflow` and als ## Getting help -For further information/help, please consult the [nf-core/smrnaseq documentation](https://nf-co.re/nf-core/smrnaseq/docs) and don't hesitate to get in touch on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). +For further information/help, please consult the [nf-core/smrnaseq documentation](https://nf-co.re/smrnaseq/docs) and don't hesitate to get in touch on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 2857708e..fb773235 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -1,24 +1,27 @@ + -## Describe the bug +## Description of the bug -A clear and concise description of what the bug is. + ## Steps to reproduce Steps to reproduce the behaviour: -1. Command line: `nextflow run ...` -2. See error: _Please provide your error message_ +1. Command line: +2. See error: ## Expected behaviour -A clear and concise description of what you expected to happen. + ## System @@ -39,4 +42,4 @@ A clear and concise description of what you expected to happen. ## Additional context -Add any other context about the problem here. + diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index cf19a32d..58594d49 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,24 +1,26 @@ + ## Is your feature request related to a problem? Please describe -A clear and concise description of what the problem is. + -Ex. I'm always frustrated when [...] + ## Describe the solution you'd like -A clear and concise description of what you want to happen. + ## Describe alternatives you've considered -A clear and concise description of any alternative solutions or features you've considered. + ## Additional context -Add any other context about the feature request here. + diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 9db6d8f3..d178daf0 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -1,3 +1,4 @@ + + ## PR checklist - [ ] This comment contains a description of changes (with reason) +- [ ] `CHANGELOG.md` is updated - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If necessary, also make a PR on the [nf-core/smrnaseq branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/smrnaseq) -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). -- [ ] Make sure your code lints (`nf-core lint .`). - [ ] Documentation in `docs` is updated -- [ ] `CHANGELOG.md` is updated -- [ ] `README.md` is updated - -**Learn more about contributing:** [CONTRIBUTING.md](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) \ No newline at end of file +- [ ] If necessary, also make a PR on the [nf-core/smrnaseq branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/smrnaseq) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml new file mode 100644 index 00000000..07ec31a5 --- /dev/null +++ b/.github/workflows/awsfulltest.yml @@ -0,0 +1,40 @@ +name: nf-core AWS full size tests +# This workflow is triggered on push to the master branch. +# It runs the -profile 'test_full' on AWS batch + +on: + release: + types: [published] + +jobs: + run-awstest: + name: Run AWS full tests + if: github.repository == 'nf-core/smrnaseq' + runs-on: ubuntu-latest + steps: + - name: Setup Miniconda + uses: goanpeca/setup-miniconda@v1.0.2 + with: + auto-update-conda: true + python-version: 3.7 + - name: Install awscli + run: conda install -c conda-forge awscli + - name: Start AWS batch job + # TODO nf-core: You can customise AWS full pipeline tests as required + # Add full size test data (but still relatively small datasets for few samples) + # on the `test_full.config` test runs with only one set of parameters + # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command + env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + run: | + aws batch submit-job \ + --region eu-west-1 \ + --job-name nf-core-smrnaseq \ + --job-queue $AWS_JOB_QUEUE \ + --job-definition $AWS_JOB_DEFINITION \ + --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml new file mode 100644 index 00000000..bba984a5 --- /dev/null +++ b/.github/workflows/awstest.yml @@ -0,0 +1,40 @@ +name: nf-core AWS test +# This workflow is triggered on push to the master branch. +# It runs the -profile 'test' on AWS batch + +on: + push: + branches: + - master + +jobs: + run-awstest: + name: Run AWS tests + if: github.repository == 'nf-core/smrnaseq' + runs-on: ubuntu-latest + steps: + - name: Setup Miniconda + uses: goanpeca/setup-miniconda@v1.0.2 + with: + auto-update-conda: true + python-version: 3.7 + - name: Install awscli + run: conda install -c conda-forge awscli + - name: Start AWS batch job + # TODO nf-core: You can customise CI pipeline run tests as required + # For example: adding multiple test runs with different parameters + # Remember that you can parallelise this by using strategy.matrix + env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + run: | + aws batch submit-job \ + --region eu-west-1 \ + --job-name nf-core-smrnaseq \ + --job-queue $AWS_JOB_QUEUE \ + --job-definition $AWS_JOB_DEFINITION \ + --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 819d8516..3e492811 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -3,14 +3,34 @@ name: nf-core branch protection # It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev` on: pull_request: - branches: - - master + branches: [master] jobs: test: - runs-on: ubuntu-18.04 + runs-on: ubuntu-latest steps: - # PRs are only ok if coming from an nf-core `dev` branch or a fork `patch` branch + # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs + if: github.repository == 'nf-core/smrnaseq' run: | - { [[ $(git remote get-url origin) == *nf-core/smrnaseq ]] && [[ ${GITHUB_HEAD_REF} = "dev" ]]; } || [[ ${GITHUB_HEAD_REF} == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/smrnaseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + Hi @${{ github.event.pull_request.user.login }}, + + It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch. + The `master` branch on nf-core repositories should always contain code from the latest release. + Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch. + + You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 77d552a2..fe56683d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,30 +1,55 @@ name: nf-core CI -# This workflow is triggered on pushes and PRs to the repository. -# It runs the pipeline with the minimal test dataset to check that it completes without any syntax errors -on: [push, pull_request] +# This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors +on: + push: + branches: + - dev + pull_request: + release: + types: [published] jobs: test: + name: Run workflow tests + # Only run on push if this is the nf-core dev branch (merged PRs) + if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/smrnaseq') }} + runs-on: ubuntu-latest env: NXF_VER: ${{ matrix.nxf_ver }} NXF_ANSI_LOG: false - runs-on: ubuntu-latest strategy: matrix: # Nextflow versions: check pipeline minimum and current latest nxf_ver: ['19.10.0', ''] steps: - - uses: actions/checkout@v2 - - name: Install Nextflow - run: | - wget -qO- get.nextflow.io | bash - sudo mv nextflow /usr/local/bin/ + - name: Check out pipeline code + uses: actions/checkout@v2 + + - name: Check if Dockerfile or Conda environment changed + uses: technote-space/get-diff-action@v1 + with: + PREFIX_FILTER: | + Dockerfile + environment.yml + + - name: Build new docker image + if: env.GIT_DIFF + run: docker build --no-cache . -t nfcore/smrnaseq:dev + - name: Pull docker image + if: ${{ !env.GIT_DIFF }} run: | docker pull nfcore/smrnaseq:dev docker tag nfcore/smrnaseq:dev nfcore/smrnaseq:dev + + - name: Install Nextflow + run: | + wget -qO- get.nextflow.io | bash + sudo mv nextflow /usr/local/bin/ + - name: Run pipeline with test data + # TODO nf-core: You can customise CI pipeline run tests as required + # For example: adding multiple test runs with different parameters + # Remember that you can parallelise this by using strategy.matrix run: | - # TODO nf-core: You can customise CI pipeline run tests as required - # (eg. adding multiple test runs with different parameters) nextflow run ${GITHUB_WORKSPACE} -profile test,docker diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1e0827a8..eb66c144 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -33,18 +33,29 @@ jobs: nf-core: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v2 + + - name: Check out pipeline code + uses: actions/checkout@v2 + - name: Install Nextflow run: | wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ + - uses: actions/setup-python@v1 with: python-version: '3.6' architecture: 'x64' + - name: Install dependencies run: | python -m pip install --upgrade pip pip install nf-core + - name: Run nf-core lint + env: + GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} run: nf-core lint ${GITHUB_WORKSPACE} + diff --git a/.github/workflows/push_dockerhub.yml b/.github/workflows/push_dockerhub.yml new file mode 100644 index 00000000..55fac4e9 --- /dev/null +++ b/.github/workflows/push_dockerhub.yml @@ -0,0 +1,39 @@ +name: nf-core Docker push +# This builds the docker image and pushes it to DockerHub +# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) +on: + push: + branches: + - dev + release: + types: [published] + +push_dockerhub: + name: Push new Docker image to Docker Hub + runs-on: ubuntu-latest + # Only run for the nf-core repo, for releases and merged PRs + if: ${{ github.repository == 'nf-core/smrnaseq' }} + env: + DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} + DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} + steps: + - name: Check out pipeline code + uses: actions/checkout@v2 + + - name: Build new docker image + run: docker build --no-cache . -t nfcore/smrnaseq:latest + + - name: Push Docker image to DockerHub (dev) + if: ${{ github.event_name == 'push' }} + run: | + echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin + docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:dev + docker push nfcore/smrnaseq:dev + + - name: Push Docker image to DockerHub (release) + if: ${{ github.event_name == 'release' }} + run: | + echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin + docker push nfcore/smrnaseq:latest + docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:${{ github.event.release.tag_name }} + docker push nfcore/smrnaseq:${{ github.event.release.tag_name }} diff --git a/.gitignore b/.gitignore index 6354f370..aa4bb5b3 100644 --- a/.gitignore +++ b/.gitignore @@ -5,4 +5,5 @@ results/ .DS_Store tests/ testing/ +testing* *.pyc diff --git a/CHANGELOG.md b/CHANGELOG.md index 668b314e..b0bee829 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ # nf-core/smrnaseq: Changelog -The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/) -and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html). +The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) +and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0.0 - [date] +## v1.0.1dev - [date] -Initial release of nf-core/smrnaseq, created with the [nf-core](http://nf-co.re/) template. +Initial release of nf-core/smrnaseq, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index cf930c8a..405fb1bf 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -40,7 +40,7 @@ Project maintainers who do not follow or enforce the Code of Conduct in good fai ## Attribution -This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [http://contributor-covenant.org/version/1/4][version] +This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version] -[homepage]: http://contributor-covenant.org -[version]: http://contributor-covenant.org/version/1/4/ +[homepage]: https://contributor-covenant.org +[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/ diff --git a/Dockerfile b/Dockerfile index 12804ea0..19704ebc 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,13 +1,17 @@ -FROM nfcore/base:1.9 +FROM nfcore/base:1.10.1 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" # Install the conda environment COPY environment.yml / -RUN conda env create -f /environment.yml && conda clean -a +RUN conda env create --quiet -f /environment.yml && conda clean -a # Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.0/bin:$PATH +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.1dev/bin:$PATH # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-smrnaseq-1.0.0 > nf-core-smrnaseq-1.0.0.yml +RUN conda env export --name nf-core-smrnaseq-1.0.1dev > nf-core-smrnaseq-1.0.1dev.yml + +# Instruct R processes to use these empty files instead of clashing with a local version +RUN touch .Rprofile +RUN touch .Renviron diff --git a/README.md b/README.md index 380046e0..2652607e 100644 --- a/README.md +++ b/README.md @@ -6,8 +6,9 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/smrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/smrnaseq/actions) [![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A519.10.0-brightgreen.svg)](https://www.nextflow.io/) -[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](http://bioconda.github.io/) +[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/smrnaseq.svg)](https://hub.docker.com/r/nfcore/smrnaseq) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23smrnaseq-4A154B?logo=slack)](https://nfcore.slack.com/channels/smrnaseq) ## Introduction @@ -15,40 +16,31 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ## Quick Start -i. Install [`nextflow`](https://nf-co.re/usage/installation) +1. Install [`nextflow`](https://nf-co.re/usage/installation) -ii. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility (please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles)) +2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ -iii. Download the pipeline and test it on a minimal dataset with a single command +3. Download the pipeline and test it on a minimal dataset with a single command: -```bash -nextflow run nf-core/smrnaseq -profile test, -``` + ```bash + nextflow run nf-core/smrnaseq -profile test, + ``` -> Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. -iv. Start running your own analysis! +4. Start running your own analysis! - + -```bash -nextflow run nf-core/smrnaseq -profile --reads '*_R{1,2}.fastq.gz' --genome GRCh37 -``` + ```bash + nextflow run nf-core/smrnaseq -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + ``` See [usage docs](docs/usage.md) for all of the available options when running the pipeline. ## Documentation -The nf-core/smrnaseq pipeline comes with documentation about the pipeline, found in the `docs/` directory: - -1. [Installation](https://nf-co.re/usage/installation) -2. Pipeline configuration - * [Local installation](https://nf-co.re/usage/local_installation) - * [Adding your own system config](https://nf-co.re/usage/adding_own_config) - * [Reference genomes](https://nf-co.re/usage/reference_genomes) -3. [Running the pipeline](docs/usage.md) -4. [Output and how to interpret the results](docs/output.md) -5. [Troubleshooting](https://nf-co.re/usage/troubleshooting) +The nf-core/smrnaseq pipeline comes with documentation about the pipeline which you can read at [https://nf-core/smrnaseq/docs](https://nf-core/smrnaseq/docs) or find in the [`docs/` directory](docs). @@ -60,7 +52,7 @@ nf-core/smrnaseq was originally written by Phil Ewels If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). -For further information or help, don't hesitate to get in touch on [Slack](https://nfcore.slack.com/channels/smrnaseq) (you can join with [this invite](https://nf-co.re/join/slack)). +For further information or help, don't hesitate to get in touch on the [Slack `#smrnaseq` channel](https://nfcore.slack.com/channels/smrnaseq) (you can join with [this invite](https://nf-co.re/join/slack)). ## Citation @@ -73,5 +65,5 @@ You can cite the `nf-core` publication as follows: > > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > -> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). +> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py index 57cc4263..a26d1ff5 100755 --- a/bin/markdown_to_html.py +++ b/bin/markdown_to_html.py @@ -4,33 +4,23 @@ import markdown import os import sys +import io + def convert_markdown(in_fn): - input_md = open(in_fn, mode="r", encoding="utf-8").read() + input_md = io.open(in_fn, mode="r", encoding="utf-8").read() html = markdown.markdown( "[TOC]\n" + input_md, - extensions = [ - 'pymdownx.extra', - 'pymdownx.b64', - 'pymdownx.highlight', - 'pymdownx.emoji', - 'pymdownx.tilde', - 'toc' - ], - extension_configs = { - 'pymdownx.b64': { - 'base_path': os.path.dirname(in_fn) - }, - 'pymdownx.highlight': { - 'noclasses': True - }, - 'toc': { - 'title': 'Table of Contents' - } - } + extensions=["pymdownx.extra", "pymdownx.b64", "pymdownx.highlight", "pymdownx.emoji", "pymdownx.tilde", "toc"], + extension_configs={ + "pymdownx.b64": {"base_path": os.path.dirname(in_fn)}, + "pymdownx.highlight": {"noclasses": True}, + "toc": {"title": "Table of Contents"}, + }, ) return html + def wrap_html(contents): header = """ @@ -83,18 +73,19 @@ def wrap_html(contents): def parse_args(args=None): parser = argparse.ArgumentParser() - parser.add_argument('mdfile', type=argparse.FileType('r'), nargs='?', - help='File to convert. Defaults to stdin.') - parser.add_argument('-o', '--out', type=argparse.FileType('w'), - default=sys.stdout, - help='Output file name. Defaults to stdout.') + parser.add_argument("mdfile", type=argparse.FileType("r"), nargs="?", help="File to convert. Defaults to stdin.") + parser.add_argument( + "-o", "--out", type=argparse.FileType("w"), default=sys.stdout, help="Output file name. Defaults to stdout." + ) return parser.parse_args(args) + def main(args=None): args = parse_args(args) converted_md = convert_markdown(args.mdfile.name) html = wrap_html(converted_md) args.out.write(html) -if __name__ == '__main__': + +if __name__ == "__main__": sys.exit(main()) diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index 36a72c77..9b2495db 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -5,16 +5,16 @@ # TODO nf-core: Add additional regexes for new tools in process get_software_versions regexes = { - 'nf-core/smrnaseq': ['v_pipeline.txt', r"(\S+)"], - 'Nextflow': ['v_nextflow.txt', r"(\S+)"], - 'FastQC': ['v_fastqc.txt', r"FastQC v(\S+)"], - 'MultiQC': ['v_multiqc.txt', r"multiqc, version (\S+)"], + "nf-core/smrnaseq": ["v_pipeline.txt", r"(\S+)"], + "Nextflow": ["v_nextflow.txt", r"(\S+)"], + "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"], + "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"], } results = OrderedDict() -results['nf-core/smrnaseq'] = 'N/A' -results['Nextflow'] = 'N/A' -results['FastQC'] = 'N/A' -results['MultiQC'] = 'N/A' +results["nf-core/smrnaseq"] = 'N/A' +results["Nextflow"] = 'N/A' +results["FastQC"] = 'N/A' +results["MultiQC"] = 'N/A' # Search each file using its regex for k, v in regexes.items(): @@ -30,10 +30,11 @@ # Remove software set to false in results for k in list(results): if not results[k]: - del(results[k]) + del results[k] # Dump to YAML -print (''' +print( + """ id: 'software_versions' section_name: 'nf-core/smrnaseq Software Versions' section_href: 'https://github.com/nf-core/smrnaseq' @@ -41,12 +42,13 @@ description: 'are collected at run time from the software output.' data: |
-''') -for k,v in results.items(): - print("
{}
{}
".format(k,v)) -print ("
") +""" +) +for k, v in results.items(): + print("
{}
{}
".format(k, v)) +print(" ") # Write out regexes as csv file: -with open('software_versions.csv', 'w') as f: - for k,v in results.items(): - f.write("{}\t{}\n".format(k,v)) +with open("software_versions.csv", "w") as f: + for k, v in results.items(): + f.write("{}\t{}\n".format(k, v)) diff --git a/conf/igenomes.config b/conf/igenomes.config index 2de92422..caeafceb 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -340,6 +340,7 @@ params { gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" mito_name = "chrM" + macs_gsize = "1.37e9" } 'dm6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" diff --git a/conf/test.config b/conf/test.config index 9ec602c0..d41aecd4 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,7 +19,7 @@ params { // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed single_end = false - readPaths = [ + input_paths = [ ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] ] diff --git a/conf/test_full.config b/conf/test_full.config new file mode 100644 index 00000000..76407f53 --- /dev/null +++ b/conf/test_full.config @@ -0,0 +1,22 @@ +/* + * ------------------------------------------------- + * Nextflow config file for running full-size tests + * ------------------------------------------------- + * Defines bundled input files and everything required + * to run a full size pipeline test. Use as follows: + * nextflow run nf-core/smrnaseq -profile test_full, + */ + +params { + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' + + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + single_end = false + readPaths = [ + ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], + ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] + ] +} diff --git a/docs/README.md b/docs/README.md index aa3edcd8..575abde1 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,12 +1,12 @@ # nf-core/smrnaseq: Documentation -The nf-core/smrnaseq documentation is split into the following files: - -1. [Installation](https://nf-co.re/usage/installation) -2. Pipeline configuration - * [Local installation](https://nf-co.re/usage/local_installation) - * [Adding your own system config](https://nf-co.re/usage/adding_own_config) - * [Reference genomes](https://nf-co.re/usage/reference_genomes) -3. [Running the pipeline](usage.md) -4. [Output and how to interpret the results](output.md) -5. [Troubleshooting](https://nf-co.re/usage/troubleshooting) +The nf-core/smrnaseq documentation is split into the following pages: + + + +* [Usage](usage.md) + * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. +* [Output](output.md) + * An overview of the different results produced by the pipeline and how to interpret them. + +You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) diff --git a/docs/output.md b/docs/output.md index 3699e5fe..0b4b8cc4 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,6 +2,8 @@ This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. + ## Pipeline overview @@ -9,35 +11,47 @@ This document describes the output produced by the pipeline. Most of the plots a The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [FastQC](#fastqc) - read quality control -* [MultiQC](#multiqc) - aggregate report, describing results of the whole pipeline +* [FastQC](#fastqc) - Read quality control +* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline +* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ## FastQC -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your reads. It provides information about the quality score distribution across your reads, the per base sequence content (%T/A/G/C). You get information about adapter contamination and other overrepresented sequences. +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. -For further reading and documentation see the [FastQC help](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. To see how your reads look after trimming, look at the FastQC reports in the `trim_galore` directory. +**Output files:** -**Output directory: `results/fastqc`** +* `fastqc/` + * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. +* `fastqc/zips/` + * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -* `sample_fastqc.html` - * FastQC report, containing quality metrics for your untrimmed raw fastq files -* `zips/sample_fastqc.zip` - * zip file containing the FastQC report, tab-delimited data file and plot images +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. ## MultiQC -[MultiQC](http://multiqc.info) is a visualisation tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in within the report data directory. +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. + +For more information about how to use MultiQC reports, see [https://multiqc.info](https://multiqc.info). + +**Output files:** + +* `multiqc/` + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats. -The pipeline has special steps which allow the software versions used to be reported in the MultiQC output for future traceability. +## Pipeline information -**Output directory: `results/multiqc`** +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. -* `Project_multiqc_report.html` - * MultiQC report - a standalone HTML file that can be viewed in your web browser -* `Project_multiqc_data/` - * Directory containing parsed statistics from the different tools used in the pipeline +**Output files:** -For more information about how to use MultiQC reports, see [http://multiqc.info](http://multiqc.info) +* `pipeline_info/` + * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. + * Documentation for interpretation of results in HTML format: `results_description.html`. diff --git a/docs/usage.md b/docs/usage.md index 8884bc15..8594f5ac 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,62 +1,15 @@ # nf-core/smrnaseq: Usage -## Table of contents - -* [Table of contents](#table-of-contents) -* [Introduction](#introduction) -* [Running the pipeline](#running-the-pipeline) - * [Updating the pipeline](#updating-the-pipeline) - * [Reproducibility](#reproducibility) -* [Main arguments](#main-arguments) - * [`-profile`](#-profile) - * [`--reads`](#--reads) - * [`--single_end`](#--single_end) -* [Reference genomes](#reference-genomes) - * [`--genome` (using iGenomes)](#--genome-using-igenomes) - * [`--fasta`](#--fasta) - * [`--igenomes_ignore`](#--igenomes_ignore) -* [Job resources](#job-resources) - * [Automatic resubmission](#automatic-resubmission) - * [Custom resource requests](#custom-resource-requests) -* [AWS Batch specific parameters](#aws-batch-specific-parameters) - * [`--awsqueue`](#--awsqueue) - * [`--awsregion`](#--awsregion) - * [`--awscli`](#--awscli) -* [Other command line parameters](#other-command-line-parameters) - * [`--outdir`](#--outdir) - * [`--email`](#--email) - * [`--email_on_fail`](#--email_on_fail) - * [`--max_multiqc_email_size`](#--max_multiqc_email_size) - * [`-name`](#-name) - * [`-resume`](#-resume) - * [`-c`](#-c) - * [`--custom_config_version`](#--custom_config_version) - * [`--custom_config_base`](#--custom_config_base) - * [`--max_memory`](#--max_memory) - * [`--max_time`](#--max_time) - * [`--max_cpus`](#--max_cpus) - * [`--plaintext_email`](#--plaintext_email) - * [`--monochrome_logs`](#--monochrome_logs) - * [`--multiqc_config`](#--multiqc_config) - ## Introduction -Nextflow handles job submissions on SLURM or other environments, and supervises running the jobs. Thus the Nextflow process must run until the pipeline is finished. We recommend that you put the process running in the background through `screen` / `tmux` or similar tool. Alternatively you can run nextflow within a cluster job submitted your job scheduler. - -It is recommended to limit the Nextflow Java virtual machines memory. We recommend adding the following line to your environment (typically in `~/.bashrc` or `~./bash_profile`): - -```bash -NXF_OPTS='-Xms1g -Xmx4g' -``` - - + ## Running the pipeline The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/smrnaseq --reads '*_R{1,2}.fastq.gz' -profile docker +nextflow run nf-core/smrnaseq --input '*_R{1,2}.fastq.gz' -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -86,7 +39,9 @@ First, go to the [nf-core/smrnaseq releases page](https://github.com/nf-core/smr This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. -## Main arguments +## Core Nextflow arguments + +> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). ### `-profile` @@ -104,11 +59,11 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles. If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. * `docker` - * A generic configuration profile to be used with [Docker](http://docker.com/) - * Pulls software from dockerhub: [`nfcore/smrnaseq`](http://hub.docker.com/r/nfcore/smrnaseq/) + * A generic configuration profile to be used with [Docker](https://docker.com/) + * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) * `singularity` - * A generic configuration profile to be used with [Singularity](http://singularity.lbl.gov/) - * Pulls software from DockerHub: [`nfcore/smrnaseq`](http://hub.docker.com/r/nfcore/smrnaseq/) + * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) + * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) * `conda` * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker or Singularity. * A generic configuration profile to be used with [Conda](https://conda.io/docs/) @@ -117,215 +72,50 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * A profile with a complete configuration for automated testing * Includes links to test data so needs no other parameters - - -### `--reads` - -Use this to specify the location of your input FastQ files. For example: - -```bash ---reads 'path/to/data/sample_*_{1,2}.fastq' -``` - -Please note the following requirements: - -1. The path must be enclosed in quotes -2. The path must have at least one `*` wildcard character -3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs. - -If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz` - -### `--single_end` - -By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--reads`. For example: - -```bash ---single_end --reads '*.fastq' -``` - -It is not possible to run a mixture of single-end and paired-end files in one run. - -## Reference genomes - -The pipeline config files come bundled with paths to the illumina iGenomes reference index files. If running with docker or AWS, the configuration is set up to use the [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/) resource. - -### `--genome` (using iGenomes) +### `-resume` -There are 31 different species supported in the iGenomes references. To run the pipeline, you must specify which to use with the `--genome` flag. +Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. -You can find the keys to specify the genomes in the [iGenomes config file](../conf/igenomes.config). Common genomes that are supported are: +You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. -* Human - * `--genome GRCh37` -* Mouse - * `--genome GRCm38` -* _Drosophila_ - * `--genome BDGP6` -* _S. cerevisiae_ - * `--genome 'R64-1-1'` +### `-c` -> There are numerous others - check the config file for more. +Specify the path to a specific config file (this is a core NextFlow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. -Note that you can use the same configuration setup to save sets of reference files for your own use, even if they are not part of the iGenomes resource. See the [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for instructions on where to save such a file. +#### Custom resource requests -The syntax for this reference configuration is as follows: +Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. - +Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config: ```nextflow -params { - genomes { - 'GRCh37' { - fasta = '' // Used if no star index given - } - // Any number of additional genomes, key is used with --genome +process { + withName: star { + memory = 32.GB } } ``` - - -### `--fasta` - -If you prefer, you can specify the full path to your reference genome when you run the pipeline: - -```bash ---fasta '[path to Fasta reference]' -``` - -### `--igenomes_ignore` - -Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`. - -## Job resources - -### Automatic resubmission - -Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. - -### Custom resource requests - -Wherever process-specific requirements are set in the pipeline, the default value can be changed by creating a custom config file. See the files hosted at [`nf-core/configs`](https://github.com/nf-core/configs/tree/master/conf) for examples. +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. -If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack). - -## AWS Batch specific parameters - -Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use [`-profile awsbatch`](https://github.com/nf-core/configs/blob/master/conf/awsbatch.config) and then specify all of the following parameters. - -### `--awsqueue` - -The JobQueue that you intend to use on AWS Batch. +If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -### `--awsregion` +### Running in the background -The AWS region in which to run your job. Default is set to `eu-west-1` but can be adjusted to your needs. +Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. -### `--awscli` +The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. -The [AWS CLI](https://www.nextflow.io/docs/latest/awscloud.html#aws-cli-installation) path in your custom AMI. Default: `/home/ec2-user/miniconda/bin/aws`. +Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. +Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). -Please make sure to also set the `-w/--work-dir` and `--outdir` parameters to a S3 storage bucket of your choice - you'll get an error message notifying you if you didn't. +#### Nextflow memory requirements -## Other command line parameters - - - -### `--outdir` - -The output directory where the results will be saved. - -### `--email` - -Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run. - -### `--email_on_fail` - -This works exactly as with `--email`, except emails are only sent if the workflow is not successful. - -### `--max_multiqc_email_size` - -Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB). - -### `-name` - -Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. - -This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always). - -**NB:** Single hyphen (core Nextflow option) - -### `-resume` - -Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. - -You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. - -**NB:** Single hyphen (core Nextflow option) - -### `-c` - -Specify the path to a specific config file (this is a core NextFlow command). - -**NB:** Single hyphen (core Nextflow option) - -Note - you can use this to override pipeline defaults. - -### `--custom_config_version` - -Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`. - -```bash -## Download and use config file with following git commid id ---custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96 -``` - -### `--custom_config_base` - -If you're running offline, nextflow will not be able to fetch the institutional config files -from the internet. If you don't need them, then this is not a problem. If you do need them, -you should download the files from the repo and tell nextflow where to find them with the -`custom_config_base` option. For example: +In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. +We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): ```bash -## Download and unzip the config files -cd /path/to/my/configs -wget https://github.com/nf-core/configs/archive/master.zip -unzip master.zip - -## Run the pipeline -cd /path/to/my/data -nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/ +NXF_OPTS='-Xms1g -Xmx4g' ``` - -> Note that the nf-core/tools helper package has a `download` command to download all required pipeline -> files + singularity containers + institutional configs in one go for you, to make this process easier. - -### `--max_memory` - -Use to set a top-limit for the default memory requirement for each process. -Should be a string in the format integer-unit. eg. `--max_memory '8.GB'` - -### `--max_time` - -Use to set a top-limit for the default time requirement for each process. -Should be a string in the format integer-unit. eg. `--max_time '2.h'` - -### `--max_cpus` - -Use to set a top-limit for the default CPU requirement for each process. -Should be a string in the format integer-unit. eg. `--max_cpus 1` - -### `--plaintext_email` - -Set to receive plain-text e-mails instead of HTML formatted. - -### `--monochrome_logs` - -Set to disable colourful command line output and live life in monochrome. - -### `--multiqc_config` - -Specify a path to a custom MultiQC configuration file. diff --git a/environment.yml b/environment.yml index ad6c9f22..c9df895a 100644 --- a/environment.yml +++ b/environment.yml @@ -1,6 +1,6 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.0.0 +name: nf-core-smrnaseq-1.0.1dev channels: - conda-forge - bioconda diff --git a/main.nf b/main.nf index 230deb4b..70a47e90 100644 --- a/main.nf +++ b/main.nf @@ -18,12 +18,12 @@ def helpMessage() { The typical command for running the pipeline is as follows: - nextflow run nf-core/smrnaseq --reads '*_R{1,2}.fastq.gz' -profile docker + nextflow run nf-core/smrnaseq --input '*_R{1,2}.fastq.gz' -profile docker Mandatory arguments: - --reads [file] Path to input data (must be surrounded with quotes) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more + --input [file] Path to input data (must be surrounded with quotes) + -profile [str] Configuration profile to use. Can use multiple (comma separated) + Available: conda, docker, singularity, test, awsbatch, and more Options: --genome [str] Name of iGenomes reference @@ -34,9 +34,10 @@ def helpMessage() { Other options: --outdir [file] The output directory where the results will be saved + --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) + --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic AWSBatch options: @@ -73,12 +74,13 @@ params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } // Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name +// this has the bonus effect of catching both -name and --name custom_runName = params.name if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { custom_runName = workflow.runName } +// Check AWS batch settings if (workflow.profile.contains('awsbatch')) { // AWSBatch sanity checking if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" @@ -93,28 +95,29 @@ if (workflow.profile.contains('awsbatch')) { ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) +ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true) /* * Create a channel for input read files */ -if (params.readPaths) { +if (params.input_paths) { if (params.single_end) { Channel - .from(params.readPaths) + .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" } + .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } .into { ch_read_files_fastqc; ch_read_files_trimming } } else { Channel - .from(params.readPaths) + .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.readPaths was empty - no input files supplied" } + .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } .into { ch_read_files_fastqc; ch_read_files_trimming } } } else { Channel - .fromFilePairs(params.reads, size: params.single_end ? 1 : 2) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.reads}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } + .fromFilePairs(params.input, size: params.single_end ? 1 : 2) + .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } .into { ch_read_files_fastqc; ch_read_files_trimming } } @@ -124,7 +127,7 @@ def summary = [:] if (workflow.revision) summary['Pipeline Release'] = workflow.revision summary['Run Name'] = custom_runName ?: workflow.runName // TODO nf-core: Report custom parameters here -summary['Reads'] = params.reads +summary['Reads'] = params.input summary['Fasta Ref'] = params.fasta summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" @@ -140,9 +143,10 @@ if (workflow.profile.contains('awsbatch')) { summary['AWS CLI'] = params.awscli } summary['Config Profile'] = workflow.profile -if (params.config_profile_description) summary['Config Description'] = params.config_profile_description -if (params.config_profile_contact) summary['Config Contact'] = params.config_profile_contact -if (params.config_profile_url) summary['Config URL'] = params.config_profile_url +if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description +if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact +if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url +summary['Config Files'] = workflow.configFiles.join(', ') if (params.email || params.email_on_fail) { summary['E-mail Address'] = params.email summary['E-mail on failure'] = params.email_on_fail @@ -174,7 +178,7 @@ Channel.from(summary.collect{ [it.key, it.value] }) * Parse software version numbers */ process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: 'copy', + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, saveAs: { filename -> if (filename.indexOf(".csv") > 0) filename else null @@ -201,7 +205,7 @@ process get_software_versions { process fastqc { tag "$name" label 'process_medium' - publishDir "${params.outdir}/fastqc", mode: 'copy', + publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, saveAs: { filename -> filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" } @@ -222,7 +226,7 @@ process fastqc { * STEP 2 - MultiQC */ process multiqc { - publishDir "${params.outdir}/MultiQC", mode: 'copy' + publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode input: file (multiqc_config) from ch_multiqc_config @@ -251,10 +255,11 @@ process multiqc { * STEP 3 - Output Description HTML */ process output_documentation { - publishDir "${params.outdir}/pipeline_info", mode: 'copy' + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode input: file output_docs from ch_output_docs + file images from ch_output_docs_images output: file "results_description.html" @@ -345,7 +350,11 @@ workflow.onComplete { log.info "[nf-core/smrnaseq] Sent summary e-mail to $email_address (sendmail)" } catch (all) { // Catch failures and try with plaintext - [ 'mail', '-s', subject, email_address ].execute() << email_txt + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html log.info "[nf-core/smrnaseq] Sent summary e-mail to $email_address (mail)" } } diff --git a/nextflow.config b/nextflow.config index c2aa9d2f..bd08e4d9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,9 +11,10 @@ params { // Workflow flags // TODO nf-core: Specify your pipeline's command line flags genome = false - reads = "data/*{1,2}.fastq.gz" + input = "data/*{1,2}.fastq.gz" single_end = false outdir = './results' + publish_dir_mode = 'copy' // Boilerplate options name = false @@ -78,9 +79,11 @@ if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' } -// Export this variable to prevent local Python libraries from conflicting with those in the container +// Export these variables to prevent local Python/R libraries from conflicting with those in the container env { PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" } // Capture exit codes from upstream processes when piping @@ -110,7 +113,7 @@ manifest { description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' nextflowVersion = '>=19.10.0' - version = '1.0.0' + version = '1.0.1dev' } // Function to ensure that resource requirements don't go beyond diff --git a/nextflow_schema.json b/nextflow_schema.json new file mode 100644 index 00000000..f5ee5c6f --- /dev/null +++ b/nextflow_schema.json @@ -0,0 +1,259 @@ +{ + "$schema": "https://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/smrnaseq/master/nextflow_schema.json", + "title": "nf-core/smrnaseq pipeline parameters", + "description": "Small RNA-Seq Best Practice Analysis Pipeline.", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": [ + "input" + ], + "properties": { + "input": { + "type": "string", + "fa_icon": "fas fa-dna", + "description": "Input FastQ files.", + "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" + }, + "single_end": { + "type": "boolean", + "description": "Specifies that the input is single-end reads.", + "fa_icon": "fas fa-align-center", + "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run." + }, + "outdir": { + "type": "string", + "description": "The output directory where the results will be saved.", + "default": "./results", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options for the reference genome indices used to align reads.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "fa_icon": "fas fa-font", + "description": "Path to FASTA genome file.", + "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." + }, + "igenomes_base": { + "type": "string", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes/", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "hidden": true, + "fa_icon": "fas fa-question-circle" + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "hidden": true, + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "mov" + ] + }, + "name": { + "type": "string", + "description": "Workflow name.", + "fa_icon": "fas fa-fingerprint", + "hidden": true, + "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "hidden": true, + "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true, + "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true, + "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true, + "help_text": "Set to disable colourful command line output and live life in monochrome." + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog", + "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.", + "fa_icon": "fas fa-users-cog" + }, + "hostnames": { + "type": "string", + "description": "Institutional configs hostname.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + }, + { + "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + } + ] +} From 361ef07de53af917769f8240bd07b24658d052a2 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 31 Jul 2020 13:26:29 +0200 Subject: [PATCH 024/146] Template update for nf-core/tools version 1.10.2 --- .github/CONTRIBUTING.md | 2 +- .github/workflows/awsfulltest.yml | 2 +- .github/workflows/branch.yml | 1 + .github/workflows/linting.yml | 9 ++++- .github/workflows/push_dockerhub.yml | 53 ++++++++++++++-------------- Dockerfile | 2 +- conf/test_full.config | 2 +- docs/usage.md | 2 +- main.nf | 2 +- 9 files changed, 42 insertions(+), 33 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index bdf8a75d..36dae17b 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -46,7 +46,7 @@ These tests are run both with the latest available version of `Nextflow` and als ## Patch -: warning: Only in the unlikely and regretful event of a release happening with a bug. +:warning: Only in the unlikely and regretful event of a release happening with a bug. * On your own fork, make a new branch `patch` based on `upstream/master`. * Fix the bug, and bump version (X.Y.Z+1). diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 07ec31a5..7d6e077f 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -21,7 +21,7 @@ jobs: run: conda install -c conda-forge awscli - name: Start AWS batch job # TODO nf-core: You can customise AWS full pipeline tests as required - # Add full size test data (but still relatively small datasets for few samples) + # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command env: diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 3e492811..5136ec88 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -17,6 +17,7 @@ jobs: # If the above check failed, post a comment on the PR explaining the failure + # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() uses: mshick/add-pr-comment@v1 diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index eb66c144..8e8d5bbc 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -57,5 +57,12 @@ jobs: GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core lint ${GITHUB_WORKSPACE} + run: nf-core -l lint_log.txt lint ${GITHUB_WORKSPACE} + + - name: Upload linting log file artifact + if: ${{ always() }} + uses: actions/upload-artifact@v2 + with: + name: linting-log-file + path: lint_log.txt diff --git a/.github/workflows/push_dockerhub.yml b/.github/workflows/push_dockerhub.yml index 55fac4e9..e7832f6d 100644 --- a/.github/workflows/push_dockerhub.yml +++ b/.github/workflows/push_dockerhub.yml @@ -8,32 +8,33 @@ on: release: types: [published] -push_dockerhub: - name: Push new Docker image to Docker Hub - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: ${{ github.repository == 'nf-core/smrnaseq' }} - env: - DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} - DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 +jobs: + push_dockerhub: + name: Push new Docker image to Docker Hub + runs-on: ubuntu-latest + # Only run for the nf-core repo, for releases and merged PRs + if: ${{ github.repository == 'nf-core/smrnaseq' }} + env: + DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} + DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} + steps: + - name: Check out pipeline code + uses: actions/checkout@v2 - - name: Build new docker image - run: docker build --no-cache . -t nfcore/smrnaseq:latest + - name: Build new docker image + run: docker build --no-cache . -t nfcore/smrnaseq:latest - - name: Push Docker image to DockerHub (dev) - if: ${{ github.event_name == 'push' }} - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:dev - docker push nfcore/smrnaseq:dev + - name: Push Docker image to DockerHub (dev) + if: ${{ github.event_name == 'push' }} + run: | + echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin + docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:dev + docker push nfcore/smrnaseq:dev - - name: Push Docker image to DockerHub (release) - if: ${{ github.event_name == 'release' }} - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push nfcore/smrnaseq:latest - docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:${{ github.event.release.tag_name }} - docker push nfcore/smrnaseq:${{ github.event.release.tag_name }} + - name: Push Docker image to DockerHub (release) + if: ${{ github.event_name == 'release' }} + run: | + echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin + docker push nfcore/smrnaseq:latest + docker tag nfcore/smrnaseq:latest nfcore/smrnaseq:${{ github.event.release.tag_name }} + docker push nfcore/smrnaseq:${{ github.event.release.tag_name }} diff --git a/Dockerfile b/Dockerfile index 19704ebc..641eadc7 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.10.1 +FROM nfcore/base:1.10.2 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" diff --git a/conf/test_full.config b/conf/test_full.config index 76407f53..f06cc1e2 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,7 +15,7 @@ params { // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed single_end = false - readPaths = [ + input_paths = [ ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] ] diff --git a/docs/usage.md b/docs/usage.md index 8594f5ac..7489f0b7 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -80,7 +80,7 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U ### `-c` -Specify the path to a specific config file (this is a core NextFlow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. +Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. #### Custom resource requests diff --git a/main.nf b/main.nf index 70a47e90..a8f1d1e2 100644 --- a/main.nf +++ b/main.nf @@ -127,7 +127,7 @@ def summary = [:] if (workflow.revision) summary['Pipeline Release'] = workflow.revision summary['Run Name'] = custom_runName ?: workflow.runName // TODO nf-core: Report custom parameters here -summary['Reads'] = params.input +summary['Input'] = params.input summary['Fasta Ref'] = params.fasta summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" From 88a3d33340414c90b96e778d627e95346b8b3ad6 Mon Sep 17 00:00:00 2001 From: lcabus-flomics Date: Tue, 25 Aug 2020 10:28:31 +0200 Subject: [PATCH 025/146] added mirdeep2 --- bin/scrape_software_versions.py | 5 +- conf/awsbatch.config | 2 +- conf/igenomes.config | 1 + conf/test.config | 24 ++--- environment.yml | 3 +- main.nf | 156 ++++++++++++++++++++++++++------ nextflow.config | 4 +- 7 files changed, 146 insertions(+), 49 deletions(-) diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index 17d5a2f8..38698ef0 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -13,8 +13,9 @@ 'Samtools': ['v_samtools.txt', r"samtools (\S+)"], 'Htseq': ['v_htseq.txt', r"version (\S+)"], 'FASTX': ['v_fastx.txt', r"Toolkit (\S+)"], - 'miRTrace': ['v_mirtrace.txt', r"mirtrace, version (\S+)"], + 'miRTrace': ['v_mirtrace.txt', r"(\S+)"], 'MultiQC': ['v_multiqc.txt', r"multiqc, version (\S+)"], + 'miRDeep2': ['v_mirdeep2.txt', r"miRDeep(\S+)"] } results = OrderedDict() results['nf-core/smrnaseq'] = 'N/A' @@ -28,7 +29,7 @@ results['FASTX'] = 'N/A' results['miRTrace'] = 'N/A' results['MultiQC'] = 'N/A' - +results['miRDeep2'] = 'N/A' # Search each file using its regex for k, v in regexes.items(): diff --git a/conf/awsbatch.config b/conf/awsbatch.config index 14af5866..f14e0a1b 100644 --- a/conf/awsbatch.config +++ b/conf/awsbatch.config @@ -14,5 +14,5 @@ params { aws.region = params.awsregion process.executor = 'awsbatch' process.queue = params.awsqueue -executor.awscli = '/home/ec2-user/miniconda/bin/aws' +aws.batch.cliPath = '/home/ec2-user/miniconda/bin/aws' params.tracedir = './' diff --git a/conf/igenomes.config b/conf/igenomes.config index 690fe048..9c53e298 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -16,6 +16,7 @@ bowtie = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BowtieIndex/genome.fa" mirtrace_species = "hsa" } 'GRCm38' { diff --git a/conf/test.config b/conf/test.config index 3c962ec3..f1b120d2 100644 --- a/conf/test.config +++ b/conf/test.config @@ -15,23 +15,13 @@ params { max_memory = 6.GB max_time = 48.h // Input data - readPaths = [ - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' - ] + + + // Genome references - bt_indices = [ - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' - ] - gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' - mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' - hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' - mirna_gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3' + gtf = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/genes.gtf' + mature = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/mature.fa' + hairpin = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/hairpin.fa' + mirna_gtf = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/hsa.gff3' mirtrace_species = "hsa" } diff --git a/environment.yml b/environment.yml index 753f672b..8d83ada8 100644 --- a/environment.yml +++ b/environment.yml @@ -29,4 +29,5 @@ dependencies: - seqkit=0.10.1 - mirtrace=1.0.0 - bioconductor-edger=3.26.5 - - bioconductor-limma=3.40.2 \ No newline at end of file + - bioconductor-limma=3.40.2 + - mirdeep2=2.0.1.2 diff --git a/main.nf b/main.nf index f400b181..6341b941 100644 --- a/main.nf +++ b/main.nf @@ -22,7 +22,7 @@ def helpMessage() { Mandatory arguments: --reads Path to input data (must be surrounded with quotes). NOTE! Paired-end data is NOT supported by this pipeline! For paired-end data, use Read 1 only - --genome Name of iGenomes reference. Not needed if --mature, --hairpin, --mirtrace_species are provided. + --genome Name of iGenomes reference. Not needed if --mature, --hairpin, --mirtrace_species and --refgenome are provided. --protocol Library preparation protocol. Default: "illumina". Can be set as "illumina", "nextflex", "qiaseq" or "cats" @@ -33,6 +33,7 @@ def helpMessage() { --mirna_gtf GFF/GTF file with coordinates positions of precursor and miRNAs. See: ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3 --bt_index Path to the bowtie 1 index files of the host reference genome. Optional. --mirtrace_species Species for miRTrace. Pre-defined when '--genome' is specified. (hsa, mmu ...) + --refgenome Path to the Fasta file of the reference genome. Trimming options --three_prime_adapter 3’ Adapter to trim. Default: None @@ -51,7 +52,8 @@ def helpMessage() { -name Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic --seq_center Text about sequencing center which will be added in the header of output bam files --maxMultiqcEmailFileSize Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - + --skip_mirdeep If this parameter is specified, it stops the detection of novel miRNAs with miRDeep2. Default: false + --miRNAs_PDF If this parameter is specified, it outputs the PDFs of the miRNAs (it occupies a lot of space). Default: false AWSBatch options: --awsqueue The AWSBatch JobQueue that needs to be set when running on AWSBatch --awsregion The AWS Region for your AWS Batch job to run on @@ -81,7 +83,7 @@ params.bt_indices = null params.mature = params.genome ? params.genomes[ params.genome ].mature ?: false : false params.hairpin = params.genome ? params.genomes[ params.genome ].hairpin ?: false : false params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtrace_species ?: false : false - +params.refgenome = params.genome ? params.genomes[ params.genome ].refgenome ?: false : false // Define regular variables so that they can be overwritten clip_R1 = params.clip_R1 @@ -107,7 +109,7 @@ if (params.protocol == "illumina"){ // three_prime_adapter = "GATCGGAAGAGCACACGTCTG" three_prime_adapter = "AAAAAAAA" } else { - //custom protocol + //custom protocol clip_R1 = params.clip_R1 three_prime_clip_R1 = params.three_prime_clip_R1 three_prime_adapter = params.three_prime_adapter @@ -133,10 +135,13 @@ if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } +if (params.refgenome) { refgenome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } + if( params.bt_index ){ bt_indices = Channel .fromPath("${params.bt_index}*", checkIfExists: true) .ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } + .into { indices_mirdeep2; indices_bowtie} } else if( params.bt_indices ){ bt_indices = Channel.from(params.readPaths).map{ file(it) }.toList() } @@ -276,6 +281,8 @@ process get_software_versions { fasta_formatter -h > v_fastx.txt mirtrace --version > v_mirtrace.txt multiqc --version > v_multiqc.txt + miRDeep2.pl -h > v_mirdeep2.txt + scrape_software_versions.py > software_versions_mqc.yaml """ } @@ -293,8 +300,8 @@ process make_bowtie_index { file hairpin from hairpin output: - file 'mature_idx.*' into mature_index - file 'hairpin_idx.*' into hairpin_index + file 'mature_idx.*' into mature_index_bowtie, mature_index_mirdeep2 + file 'hairpin_idx.*' into hairpin_index_bowtie, hairpin_index_mirdeep2 file 'hairpin_idx.fa' into hairpin_mirtop script: @@ -349,7 +356,7 @@ process trim_galore { file reads from raw_reads_trimgalore output: - file '*.gz' into trimmed_reads_bowtie, trimmed_reads_collapse, trimmed_reads_bowtie_ref, trimmed_reads_insertsize + file '*.gz' into trimmed_reads_bowtie, trimmed_reads_collapse, trimmed_reads_bowtie_ref, trimmed_reads_insertsize, trimmed_zipped_reads_mirdeep2 file '*trimming_report.txt' into trimgalore_results file "*_fastqc.{zip,html}" into trimgalore_fastqc_reports @@ -391,7 +398,7 @@ process insertsize { process collapse { label 'process_medium' tag "$reads" - + input: file reads from trimmed_reads_collapse @@ -417,7 +424,7 @@ process bowtie_miRBase_mature { input: file reads from trimmed_reads_bowtie - file index from mature_index + file index from mature_index_bowtie output: file '*.mature.bam' into miRBase_mature_bam @@ -456,7 +463,7 @@ process bowtie_miRBase_hairpin { input: file reads from mature_unmapped_reads - file index from hairpin_index + file index from hairpin_index_bowtie output: file '*.hairpin.bam' into miRBase_hairpin_bam, miRBase_hairpin_bam_mirtop @@ -491,10 +498,10 @@ process bowtie_miRBase_hairpin { process bowtie_miRBase_hairpin_collapsed { label 'process_medium' tag "$reads" - + input: file reads from collapsed_fasta - file index from hairpin_index + file index from hairpin_index_bowtie output: file '*.bam' into miRBase_hairpin_collapse_bam @@ -579,35 +586,35 @@ process mirtop_bam_hairpin { label 'process_medium' tag "$input" publishDir "${params.outdir}", mode: 'copy' - + when: mirna_gtf - + input: file input from miRBase_hairpin_collapse_bam.collect() file hairpin from hairpin_mirtop file gtf from mirna_gtf - + output: file "mirtop/mirtop.gff" into mirtop_gff file "mirtop/mirtop.tsv" into mirtop_tsv file "mirtop/mirna.tsv" into mirna_tsv file "mirtop/mirtop_rawData.tsv" into isomir_tsv - + script: """ mirtop gff --hairpin $hairpin --gtf $gtf -o mirtop --sps $params.mirtrace_species $input mirtop counts --hairpin $hairpin --gtf $gtf -o mirtop --sps $params.mirtrace_species --add-extra --gff mirtop/mirtop.gff mirtop export --format isomir --hairpin $hairpin --gtf $gtf --sps $params.mirtrace_species -o mirtop mirtop/mirtop.gff - collapse_mirtop.r mirtop/mirtop.tsv - """ + collapse_mirtop.r mirtop/mirtop.tsv + """ } /* * STEP 6.1 and 6.2 IF A GENOME SPECIFIED ONLY! */ -if( params.bt_index ) { +if( params.genome ) { /* * STEP 6.1 - Bowtie 1 against reference genome @@ -619,7 +626,7 @@ if( params.bt_index ) { input: file reads from trimmed_reads_bowtie_ref - file indices from bt_indices.collect() + file indices from indices_bowtie.collect() output: file '*.genome.bam' into bowtie_bam, bowtie_bam_for_unmapped @@ -649,7 +656,7 @@ if( params.bt_index ) { tag "$input" publishDir "${params.outdir}/bowtie_ref", mode: 'copy' - input: + input: file input from bowtie_bam output: @@ -694,15 +701,110 @@ if( params.bt_index ) { } else{ ch_genome_bam_flagstat_mqc = Channel.empty() } +/* + * STEP 7.0, 7.1 and 7.2 if skip_mirdeep is not specified + */ +if (! params.skip_mirdeep){ + /* + * STEP 7.0 - unzip trim galore reads + */ + + process uncompress_trimmed_reads{ + label 'process_low' + input: + path reads from trimmed_zipped_reads_mirdeep2 + + output: + path "$unzip" into trimmed_reads_mirdeep2 + + script: + unzip = reads.toString() - '.gz' + """ + pigz -f -d -p $task.cpus $reads + """ + + } + + + /* + * STEP 7.1 - miRDeep2 mapper + */ + process mapper_mirdeep2 { + label 'process_medium' + tag "$reads" + publishDir "${params.outdir}/mirdeep2/mapper", mode: 'copy' + + input: + file reads from trimmed_reads_mirdeep2 + file indices from indices_mirdeep2.collect() + + + output: + + file '*_collapsed.fa' into mirdeep_reads_collapsed + file '*reads_vs_refdb.arf' into reads_vs_refdb + + script: + index_base = indices.toString().tokenize(' ')[0].tokenize('.')[0] + + """ + mapper.pl \\ + $reads \\ + -e \\ + -h \\ + -i \\ + -j \\ + -m \\ + -p $index_base \\ + -s ${reads.baseName}_collapsed.fa \\ + -t ${reads.baseName}reads_vs_refdb.arf \\ + -o 4 + """ + } + + /* + * STEP 7.2 - miRDeep2 novel mirnas + */ + process mirdeep2 { + label 'process_medium' + publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' + + input: + file refgenome from refgenome + file reads_collapsed from mirdeep_reads_collapsed + file reads_vs_refdb from reads_vs_refdb + file mature from mature + file hairpin from hairpin + output: + + file 'result*.{bed,csv,html}' + if (params.miRNAs_PDF) {file 'pdfs*'} + + + script: + + """ + miRDeep2.pl \\ + $reads_collapsed \\ + $refgenome \\ + $reads_vs_refdb \\ + $mature \\ + none \\ + $hairpin \\ + -d \\ + -z _${reads_collapsed.simpleName} + """ + } +} /* - * STEP 7 - miRTrace + * STEP 8 - miRTrace */ process mirtrace { tag "$reads" publishDir "${params.outdir}/miRTrace", mode: 'copy' - + input: file reads from raw_reads_mirtrace.collect() @@ -715,7 +817,7 @@ process mirtrace { for i in $reads do path=\$(realpath \${i}) - prefix=\$(echo \${i} | sed -e "s/.gz//" -e "s/.fastq//" -e "s/.fq//" -e "s/_val_1//" -e "s/_trimmed//" -e "s/_R1//" -e "s/.R1//") + prefix=\$(echo \${i} | sed -e 's/.gz//' -e 's/.fastq//' -e 's/.fq//' -e 's/_val_1//' -e 's/_trimmed//' -e 's/_R1//' -e 's/.R1//') echo \$path","\$prefix done > mirtrace_config @@ -732,7 +834,7 @@ process mirtrace { /* - * STEP 8 - MultiQC + * STEP 9 - MultiQC */ process multiqc { publishDir "${params.outdir}/MultiQC", mode: 'copy' @@ -764,7 +866,7 @@ process multiqc { /* - * STEP 9 - Output Description HTML + * STEP 10 - Output Description HTML */ process output_documentation { publishDir "${params.outdir}/pipeline_info", mode: 'copy' @@ -917,7 +1019,7 @@ def nfcoreHeader(){ ${c_green}`._,._,\'${c_reset} ${c_purple} nf-core/smrnaseq v${workflow.manifest.version}${c_reset} ${c_dim}----------------------------------------------------${c_reset} - """.stripIndent() + """.stripIndent() } def checkHostname(){ diff --git a/nextflow.config b/nextflow.config index fc8f88b7..5e747080 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,8 +21,10 @@ params { skipQC = false skipFastqc = false skipMultiqc = false + skip_mirdeep = false saveReference = true seq_center = "" + miRNAs_PDF = false // Boilerplate options @@ -48,7 +50,7 @@ params { // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev -process.container = 'nfcore/smrnaseq:1.0.0' +process.container = 'lluccabus/smrnaseq' // Load base.config by default for all pipelines includeConfig 'conf/base.config' From 88465ab6839cdc11e5570e3557f289a9f45f918e Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 31 Aug 2020 22:23:23 +0200 Subject: [PATCH 026/146] Update main.nf MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Fix to hsa for miRtrace (no pha 👎 ) --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index f400b181..178a1ce5 100644 --- a/main.nf +++ b/main.nf @@ -720,7 +720,7 @@ process mirtrace { done > mirtrace_config mirtrace qc \\ - --species $params.mirtrace_species \\ + --species hsa \\ $primer \\ --protocol $protocol \\ --config mirtrace_config \\ From a5bad7a581a0f933d58775b58606cfaa1a4214f7 Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 31 Aug 2020 22:56:48 +0200 Subject: [PATCH 027/146] Update edgeR_miRBase.r Add saving counts and calculating logTPM --- bin/edgeR_miRBase.r | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/bin/edgeR_miRBase.r b/bin/edgeR_miRBase.r index 4fa12c25..8917050a 100755 --- a/bin/edgeR_miRBase.r +++ b/bin/edgeR_miRBase.r @@ -69,11 +69,22 @@ for (i in 1:2) { # Remove genes with 0 reads in all samples row_sub = apply(data, 1, function(row) all(row ==0 )) data<-data[!row_sub,] + write.csv(t(data),file=paste(header,"_counts.csv",sep="")) # Normalization dataDGE<-DGEList(counts=data,genes=rownames(data)) o <- order(rowSums(dataDGE$counts), decreasing=TRUE) dataDGE <- dataDGE[o,] + + # Save log10(TPM) + tpm = cpm(dataDGE, normalized.lib.sizes=F, log = F, prior.count = 0.001) + tpm = tpm + 0.001 + tpm = log10(tpm) + ttpm = t(tpm) + write.table(ttpm,file=paste(header,"_logtpm.txt",sep=""),sep='\t',quote=FALSE) + write.csv(ttpm,file=paste(header,"_logtpm.csv",sep="")) + + # TMM dataNorm <- calcNormFactors(dataDGE) # Print normalized read counts to file From fa7ef1f1ce11df9aa147f73a2a0a5b34bc190455 Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 31 Aug 2020 23:17:58 +0200 Subject: [PATCH 028/146] Update main.nf Hardcode hsa and add TPMs --- main.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/main.nf b/main.nf index 178a1ce5..ea680ccb 100644 --- a/main.nf +++ b/main.nf @@ -564,7 +564,7 @@ process edgeR_mirna { file input_files from miRBase_counts.toSortedList() output: - file '*.{txt,pdf}' into edgeR_miRBase_results + file '*.{txt,pdf,csv}' into edgeR_miRBase_results script: """ @@ -596,9 +596,9 @@ process mirtop_bam_hairpin { script: """ - mirtop gff --hairpin $hairpin --gtf $gtf -o mirtop --sps $params.mirtrace_species $input - mirtop counts --hairpin $hairpin --gtf $gtf -o mirtop --sps $params.mirtrace_species --add-extra --gff mirtop/mirtop.gff - mirtop export --format isomir --hairpin $hairpin --gtf $gtf --sps $params.mirtrace_species -o mirtop mirtop/mirtop.gff + mirtop gff --hairpin $hairpin --gtf $gtf -o mirtop --sps hsa $input + mirtop counts --hairpin $hairpin --gtf $gtf -o mirtop --sps hsa --add-extra --gff mirtop/mirtop.gff + mirtop export --format isomir --hairpin $hairpin --gtf $gtf --sps hsa -o mirtop mirtop/mirtop.gff collapse_mirtop.r mirtop/mirtop.tsv """ } From 90342b6b9b7d25c7d9e5ac0278bdba4146dffe5b Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 31 Aug 2020 23:52:11 +0200 Subject: [PATCH 029/146] Update main.nf --- main.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index ea680ccb..2087fd44 100644 --- a/main.nf +++ b/main.nf @@ -115,7 +115,8 @@ if (params.protocol == "illumina"){ } if (!params.mirna_gtf && params.mirtrace_species){ - mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) + // mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) + mirna_gtf = file(params.mirna_gtf, checkIfExists: true) }else if (params.mirna_gtf) { mirna_gtf = file(params.mirna_gtf, checkIfExists: true) }else{ From 2200b1c74a4c06db479b5b5393fafd57bff26311 Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Tue, 1 Sep 2020 01:32:55 +0200 Subject: [PATCH 030/146] Update main.nf --- main.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 2087fd44..ea680ccb 100644 --- a/main.nf +++ b/main.nf @@ -115,8 +115,7 @@ if (params.protocol == "illumina"){ } if (!params.mirna_gtf && params.mirtrace_species){ - // mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) - mirna_gtf = file(params.mirna_gtf, checkIfExists: true) + mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) }else if (params.mirna_gtf) { mirna_gtf = file(params.mirna_gtf, checkIfExists: true) }else{ From 3e31b38f623d0c7c2df98a49b2b7abb3673d21f6 Mon Sep 17 00:00:00 2001 From: lcabus-flomics Date: Mon, 21 Sep 2020 15:31:09 +0200 Subject: [PATCH 031/146] changed multiple files --- README.md | 7 ++-- conf/base.config | 14 ++++---- conf/igenomes.config | 18 +++++++++- conf/test.config | 27 +++++++++++---- docs/output.md | 14 +++++++- main.nf | 79 +++++++++++++++++++++++++++++++++++++------- 6 files changed, 130 insertions(+), 29 deletions(-) diff --git a/README.md b/README.md index 342f11b6..8b23c0e8 100644 --- a/README.md +++ b/README.md @@ -35,8 +35,11 @@ The pipeline is built using Nextflow, a workflow tool to run tasks across multip 3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop)) 6. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml)) 1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) -7. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace)) -8. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/)) +7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`] https://www.mdc-berlin.de/content/mirdeep2-documentation) + 1. Mapping against reference genome with the mapper module + 2. Known and novel miRNA discovery with the mirdeep2 module +8. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace)) +9. Present QC for raw read, alignment, and expression results ([`MultiQC`](http://multiqc.info/)) ### Documentation diff --git a/conf/base.config b/conf/base.config index c7ae2f9c..ec944ffa 100644 --- a/conf/base.config +++ b/conf/base.config @@ -12,7 +12,7 @@ process { cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 8.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 2.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } @@ -22,17 +22,17 @@ process { // Resource requirements withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 16.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 32.GB * task.attempt, 'memory' ) } + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 8 * task.attempt, 'cpus' ) } - memory = { check_max( 64.GB * task.attempt, 'memory' ) } + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } } @@ -42,5 +42,5 @@ params { max_memory = 128.GB max_cpus = 16 max_time = 240.h - igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_base = 's3://ngi-igenomes/igenomes' } diff --git a/conf/igenomes.config b/conf/igenomes.config index 9c53e298..000127b9 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -16,7 +16,7 @@ bowtie = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BowtieIndex/genome.fa" + refgenome = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "hsa" } 'GRCm38' { @@ -25,6 +25,7 @@ bowtie = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "mmu" } 'TAIR10' { @@ -33,6 +34,7 @@ bowtie = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ath" } 'UMD3.1' { @@ -41,6 +43,7 @@ bowtie = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "bta" } 'WBcel235' { @@ -49,6 +52,7 @@ bowtie = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "cel" } 'CanFam3.1' { @@ -57,6 +61,7 @@ bowtie = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "cfa" } 'GRCz10' { @@ -73,6 +78,7 @@ bowtie = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "dme" } 'EquCab2' { @@ -81,6 +87,7 @@ bowtie = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "eca" } 'Galgal4' { @@ -89,6 +96,7 @@ bowtie = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "gga" } 'Gm01' { @@ -97,6 +105,7 @@ bowtie = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "gma" } 'Mmul_1' { @@ -105,6 +114,7 @@ bowtie = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "mml" } 'IRGSP-1.0' { @@ -113,6 +123,7 @@ bowtie = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "osa" } 'CHIMP2.1.4' { @@ -121,6 +132,7 @@ bowtie = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ptr" } 'Rnor_6.0' { @@ -129,6 +141,7 @@ bowtie = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "rno" } 'Sbi1' { @@ -137,6 +150,7 @@ bowtie = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "sbi" } 'Sscrofa10.2' { @@ -145,6 +159,7 @@ bowtie = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ssc" } 'AGPv3' { @@ -153,6 +168,7 @@ bowtie = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BowtieIndex/genome" fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta" gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + refgenome = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "zma" } } diff --git a/conf/test.config b/conf/test.config index f1b120d2..473d7b1f 100644 --- a/conf/test.config +++ b/conf/test.config @@ -15,13 +15,28 @@ params { max_memory = 6.GB max_time = 48.h // Input data - + + readPaths = [ + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' + ] // Genome references - gtf = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/genes.gtf' - mature = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/mature.fa' - hairpin = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/hairpin.fa' - mirna_gtf = '/home/lcabus/Flomics/smrnaseq_test/mapper/test/references/hsa.gff3' - mirtrace_species = "hsa" + skip_mirdeep = false + bt_indices = [ + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' +] +gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' +mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' +hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' +mirna_gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3' +mirtrace_species = "hsa" + } diff --git a/docs/output.md b/docs/output.md index 79757fff..e3ccb4d2 100644 --- a/docs/output.md +++ b/docs/output.md @@ -15,6 +15,7 @@ and processes data using the following steps: * [edgeR](#edger) - normalization, MDS plot and sample pairwise distance heatmap * [Bowtie](#bowtie) - alignment against reference genome for QC purpose * [mirtop](#mirtop) - miRNA and isomiR annotation +* [miRDeep2](#mirdeep2) - known and novel miRNA annotation * [miRTrace](#mirtrace) - a comprehensive tool for QC purpose * [MultiQC](#multiqc) - aggregate report, describing results of the whole pipeline @@ -123,13 +124,24 @@ This is an example of the output we can get: ## mirtop [mirtop](https://github.com/miRTop/mirtop) is used to parse the BAM files from `bowtie` alignment, and produce a [mirgff3](https://github.com/miRTop/mirGFF3) file with information about miRNAs and isomirs. -** Output directory: `results/mirtop` ** +**Output directory: `results/mirtop`** * `mirtop.gff`: [mirgff3](https://github.com/miRTop/mirGFF3) file * `mirtop.tsv`: tabular file of the previous file for easy integration with downstream analysis. * `mirtop_rawData.tsv`: File compatible with [isomiRs](http://lpantano.github.io/isomiRs/reference/IsomirDataSeqFromMirtop.html) Bioconductor package to perform isomiRs analysis. * `mirna.tsv`: tabular file with miRNA counts after summarizing unique isomiRs for each miRNA +##miRDeep2 +[miRDeep2] (https://www.mdc-berlin.de/content/mirdeep2-documentation) is used for the identification of novel and known miRNAs in deep sequencing data. + +**Output directory: `results/mirdeep2`** + +* `mapper/sample_trimmed_collapsed.fa`: trimmed reads collapsed in order to reduce disk space. +* `mapper/sample_reads_vs_refdb.arf`: file with the mapped reads to the genome. +* `mirdeep/timestamp_sample.bed`: file with the known and novel miRNAs in bed format. +* `mirdeep/timestamp_sample.csv`: file with an overview of all detected miRNAs (known and novel) in csv format. +* `mirdeep/timestamp_sample.html`: file with an overview of all detected miRNAs (known and novel) in html format. + ## miRTrace [miRTrace](https://github.com/friedlanderlab/mirtrace) is a quality control specifically for small RNA sequencing data (smRNA-Seq). Each sample is characterized by profiling sequencing quality, read length, sequencing depth and miRNA complexity and also the amounts of miRNAs versus undesirable sequences (derived from tRNAs, rRNAs and sequencing artifacts). diff --git a/main.nf b/main.nf index 6341b941..4051cd41 100644 --- a/main.nf +++ b/main.nf @@ -33,7 +33,8 @@ def helpMessage() { --mirna_gtf GFF/GTF file with coordinates positions of precursor and miRNAs. See: ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3 --bt_index Path to the bowtie 1 index files of the host reference genome. Optional. --mirtrace_species Species for miRTrace. Pre-defined when '--genome' is specified. (hsa, mmu ...) - --refgenome Path to the Fasta file of the reference genome. + --refgenome Path to the Fasta file of the reference genome. + --references_parsed Path to the parsed reference folder containing the files for the miRDeep2. Trimming options --three_prime_adapter 3’ Adapter to trim. Default: None @@ -53,7 +54,7 @@ def helpMessage() { --seq_center Text about sequencing center which will be added in the header of output bam files --maxMultiqcEmailFileSize Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) --skip_mirdeep If this parameter is specified, it stops the detection of novel miRNAs with miRDeep2. Default: false - --miRNAs_PDF If this parameter is specified, it outputs the PDFs of the miRNAs (it occupies a lot of space). Default: false + AWSBatch options: --awsqueue The AWSBatch JobQueue that needs to be set when running on AWSBatch --awsregion The AWS Region for your AWS Batch job to run on @@ -79,7 +80,6 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome // Genome options params.bt_index = params.genome ? params.genomes[ params.genome ].bowtie ?: false : false -params.bt_indices = null params.mature = params.genome ? params.genomes[ params.genome ].mature ?: false : false params.hairpin = params.genome ? params.genomes[ params.genome ].hairpin ?: false : false params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtrace_species ?: false : false @@ -135,14 +135,40 @@ if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } -if (params.refgenome) { refgenome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } +if (!params.skip_mirdeep){ + if (params.refgenome) { refgenome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } +} + +if (params.references_parsed){ + refgenome = Channel + .fromPath("$params.references_parsed/genome.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} + .into { reference_genome } + + bowtie = Channel + .fromPath("$params.references_parsed/genome", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} + .into { indices_bowtie; indices_mirdeep2 } + + hairpin = Channel + .fromPath("$params.references_parsed/hairpin.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed hairpin.fa not found: ${references_parsed}"} + + mature = Channel + .fromPath("$params.references_parsed/mature.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed mature.fa not found: ${references_parsed}"} + +} if( params.bt_index ){ + if (params.skip_mirdeep){ bt_indices = Channel .fromPath("${params.bt_index}*", checkIfExists: true) .ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } .into { indices_mirdeep2; indices_bowtie} -} else if( params.bt_indices ){ + } +} +else if( params.bt_indices ){ bt_indices = Channel.from(params.readPaths).map{ file(it) }.toList() } if( !params.bt_index) { @@ -202,6 +228,7 @@ summary["Trim 5' R1"] = clip_R1 summary["Trim 3' R1"] = three_prime_clip_R1 summary['miRBase mature'] = params.mature summary['miRBase hairpin'] = params.hairpin +summary['Reference Genome'] = params.refgenome if(params.bt_index) summary['Bowtie Index for Ref'] = params.bt_index summary['Save Reference'] = params.saveReference ? 'Yes' : 'No' summary['Protocol'] = params.protocol @@ -300,8 +327,8 @@ process make_bowtie_index { file hairpin from hairpin output: - file 'mature_idx.*' into mature_index_bowtie, mature_index_mirdeep2 - file 'hairpin_idx.*' into hairpin_index_bowtie, hairpin_index_mirdeep2 + file 'mature_idx.*' into mature_index_bowtie + file 'hairpin_idx.*' into hairpin_index_bowtie file 'hairpin_idx.fa' into hairpin_mirtop script: @@ -320,6 +347,36 @@ process make_bowtie_index { """ } +if (! params.references_parsed & !params.skip_mirdeep){ + /* + * STEP 0.0.1 - Parse Genome.fa and generate bowtie indices + */ + process bowtie_indices { + label 'process_medium' + publishDir "${params.outdir}/references", mode: 'symlink' + + input: + file refgenome from refgenome + file mature from mature + file hairpin from hairpin + + output: + file 'genome.fa' into reference_genome + file '*.ebwt' into indices_bowtie,indices_mirdeep2 + file 'hairpin.fa' into hairpin + file 'mature.fa' into mature + + script: + """ + sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome + sed -i 's, ,_,g' $hairpin + sed -i 's, ,_,g' $mature + bowtie-build $refgenome genome + """ + + } +} + /* * STEP 1 - FastQC */ @@ -726,7 +783,6 @@ if (! params.skip_mirdeep){ } - /* * STEP 7.1 - miRDeep2 mapper */ @@ -741,7 +797,6 @@ if (! params.skip_mirdeep){ output: - file '*_collapsed.fa' into mirdeep_reads_collapsed file '*reads_vs_refdb.arf' into reads_vs_refdb @@ -758,7 +813,7 @@ if (! params.skip_mirdeep){ -m \\ -p $index_base \\ -s ${reads.baseName}_collapsed.fa \\ - -t ${reads.baseName}reads_vs_refdb.arf \\ + -t ${reads.baseName}_reads_vs_refdb.arf \\ -o 4 """ } @@ -771,15 +826,15 @@ if (! params.skip_mirdeep){ publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' input: - file refgenome from refgenome + file refgenome from reference_genome file reads_collapsed from mirdeep_reads_collapsed file reads_vs_refdb from reads_vs_refdb file mature from mature file hairpin from hairpin + output: file 'result*.{bed,csv,html}' - if (params.miRNAs_PDF) {file 'pdfs*'} script: From 645fbe48465218dc17e4fddb81675f8a562312c1 Mon Sep 17 00:00:00 2001 From: lcabus-flomics Date: Mon, 21 Sep 2020 15:41:57 +0200 Subject: [PATCH 032/146] updated readme.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 8b23c0e8..032b6d36 100644 --- a/README.md +++ b/README.md @@ -35,7 +35,7 @@ The pipeline is built using Nextflow, a workflow tool to run tasks across multip 3. miRNA and isomiR annotation from step 4.1 ([`mirtop`](https://github.com/miRTop/mirtop)) 6. Alignment against host reference genome ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml)) 1. Post-alignment processing of alignment against host reference genome ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) -7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`] https://www.mdc-berlin.de/content/mirdeep2-documentation) +7. Novel miRNAs and known miRNAs discovery ([`MiRDeep2`](https://www.mdc-berlin.de/content/mirdeep2-documentation)) 1. Mapping against reference genome with the mapper module 2. Known and novel miRNA discovery with the mirdeep2 module 8. miRNA quality control ([`mirtrace`](https://github.com/friedlanderlab/mirtrace)) From e7057559405dfc573bf9b95b9975426682154ab4 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 28 Sep 2020 15:48:21 +0200 Subject: [PATCH 033/146] update base.config --- conf/base.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/base.config b/conf/base.config index ec944ffa..a6ed1a64 100644 --- a/conf/base.config +++ b/conf/base.config @@ -22,17 +22,17 @@ process { // Resource requirements withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + memory = { check_max( 16.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + cpus = { check_max( 4 * task.attempt, 'cpus' ) } + memory = { check_max( 32.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + cpus = { check_max( 8 * task.attempt, 'cpus' ) } + memory = { check_max( 64.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } } From a548276d94cd24aac9754dcfa81ff5f7eea2becb Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?lluc=20cab=C3=BAs?= Date: Tue, 29 Sep 2020 11:16:04 +0200 Subject: [PATCH 034/146] fixed skip_mirdeep --- conf/test.config | 2 +- main.nf | 125 ++++++++++++++++++++++++----------------------- nextflow.config | 3 +- 3 files changed, 66 insertions(+), 64 deletions(-) diff --git a/conf/test.config b/conf/test.config index 473d7b1f..05da5471 100644 --- a/conf/test.config +++ b/conf/test.config @@ -12,7 +12,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on Travis max_cpus = 2 - max_memory = 6.GB + max_memory = 10.GB max_time = 48.h // Input data diff --git a/main.nf b/main.nf index 4051cd41..3e435ac1 100644 --- a/main.nf +++ b/main.nf @@ -125,49 +125,52 @@ if (!params.mirna_gtf && params.mirtrace_species){ } // Validate inputs + if( !params.mature || !params.hairpin ){ exit 1, "Missing mature / hairpin reference indexes! Is --genome specified?" } -if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - -if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } +if (params.skip_mirdeep){ + if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } -if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } + if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } -if (!params.skip_mirdeep){ - if (params.refgenome) { refgenome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } + if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } } +else{ + if (params.references_parsed){ + refgenome = file("$params.references_parsed/genome.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} + + indices_mirdeep2 = Channel + .fromPath("$params.references_parsed/genome.*.ebwt", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed genome indices not found: ${references_parsed}"} + + hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed hairpin.fa not found: ${references_parsed}"} -if (params.references_parsed){ - refgenome = Channel - .fromPath("$params.references_parsed/genome.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} - .into { reference_genome } + mature = file("$params.references_parsed/mature.fa", checkIfExists: true) + .ifEmpty { exit 1, "Reference parsed mature.fa not found: ${references_parsed}"} - bowtie = Channel - .fromPath("$params.references_parsed/genome", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} - .into { indices_bowtie; indices_mirdeep2 } + } + else{ + if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - hairpin = Channel - .fromPath("$params.references_parsed/hairpin.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed hairpin.fa not found: ${references_parsed}"} + if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } + + if (params.refgenome) {reference_genome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } + + } - mature = Channel - .fromPath("$params.references_parsed/mature.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed mature.fa not found: ${references_parsed}"} } if( params.bt_index ){ - if (params.skip_mirdeep){ - bt_indices = Channel - .fromPath("${params.bt_index}*", checkIfExists: true) - .ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } - .into { indices_mirdeep2; indices_bowtie} - } + bt_indices = Channel + .fromPath("${params.bt_index}*", checkIfExists: true) + .ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } } + else if( params.bt_indices ){ bt_indices = Channel.from(params.readPaths).map{ file(it) }.toList() } @@ -314,6 +317,36 @@ process get_software_versions { """ } + +if (! params.references_parsed & !params.skip_mirdeep){ + /* + * PREPROCESSING - Parse genome.fa and build Bowtie index for refgenome + */ + process bowtie_indices { + label 'process_medium' + publishDir "${params.outdir}/references", mode: 'symlink' + + input: + file refgenome from reference_genome + file mature from reference_mature + file hairpin from reference_hairpin + + output: + file 'genome.fa' into refgenome + file 'genome.*.ebwt' into indices_mirdeep2 + file 'hairpin.fa' into hairpin + file 'mature.fa' into mature + + script: + """ + sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome + sed -i 's, ,_,g' $hairpin + sed -i 's, ,_,g' $mature + bowtie-build $refgenome genome + """ + + } +} /* * PREPROCESSING - Build Bowtie index for mature and hairpin */ @@ -328,7 +361,7 @@ process make_bowtie_index { output: file 'mature_idx.*' into mature_index_bowtie - file 'hairpin_idx.*' into hairpin_index_bowtie + file 'hairpin_idx.*' into hairpin_index_bowtie, hairpin_index_bowtie_2 file 'hairpin_idx.fa' into hairpin_mirtop script: @@ -347,36 +380,6 @@ process make_bowtie_index { """ } -if (! params.references_parsed & !params.skip_mirdeep){ - /* - * STEP 0.0.1 - Parse Genome.fa and generate bowtie indices - */ - process bowtie_indices { - label 'process_medium' - publishDir "${params.outdir}/references", mode: 'symlink' - - input: - file refgenome from refgenome - file mature from mature - file hairpin from hairpin - - output: - file 'genome.fa' into reference_genome - file '*.ebwt' into indices_bowtie,indices_mirdeep2 - file 'hairpin.fa' into hairpin - file 'mature.fa' into mature - - script: - """ - sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome - sed -i 's, ,_,g' $hairpin - sed -i 's, ,_,g' $mature - bowtie-build $refgenome genome - """ - - } -} - /* * STEP 1 - FastQC */ @@ -558,7 +561,7 @@ process bowtie_miRBase_hairpin_collapsed { input: file reads from collapsed_fasta - file index from hairpin_index_bowtie + file index from hairpin_index_bowtie_2 output: file '*.bam' into miRBase_hairpin_collapse_bam @@ -671,7 +674,7 @@ process mirtop_bam_hairpin { /* * STEP 6.1 and 6.2 IF A GENOME SPECIFIED ONLY! */ -if( params.genome ) { +if( params.bt_index ) { /* * STEP 6.1 - Bowtie 1 against reference genome @@ -683,7 +686,7 @@ if( params.genome ) { input: file reads from trimmed_reads_bowtie_ref - file indices from indices_bowtie.collect() + file indices from bt_indices.collect() output: file '*.genome.bam' into bowtie_bam, bowtie_bam_for_unmapped diff --git a/nextflow.config b/nextflow.config index 5e747080..6fce5a60 100644 --- a/nextflow.config +++ b/nextflow.config @@ -24,8 +24,7 @@ params { skip_mirdeep = false saveReference = true seq_center = "" - miRNAs_PDF = false - + references_parsed = false // Boilerplate options name = false From d0d7a54f72465e2cf4e80bb180c0acc2e00b5df6 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?lluc=20cab=C3=BAs?= Date: Wed, 30 Sep 2020 10:02:43 +0200 Subject: [PATCH 035/146] chenged main.ng --- conf/test.config | 6 +++--- main.nf | 17 +++-------------- 2 files changed, 6 insertions(+), 17 deletions(-) diff --git a/conf/test.config b/conf/test.config index 05da5471..1e0b638d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -12,16 +12,16 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on Travis max_cpus = 2 - max_memory = 10.GB + max_memory = 6.GB max_time = 48.h // Input data - + readPaths = [ 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' ] - + // Genome references skip_mirdeep = false diff --git a/main.nf b/main.nf index 3e435ac1..1c7c23f8 100644 --- a/main.nf +++ b/main.nf @@ -132,32 +132,21 @@ if( !params.mature || !params.hairpin ){ if (params.skip_mirdeep){ if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } - if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } } else{ if (params.references_parsed){ refgenome = file("$params.references_parsed/genome.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed genome not found: ${references_parsed}"} - + hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) + mature = file("$params.references_parsed/mature.fa", checkIfExists: true) indices_mirdeep2 = Channel .fromPath("$params.references_parsed/genome.*.ebwt", checkIfExists: true) .ifEmpty { exit 1, "Reference parsed genome indices not found: ${references_parsed}"} - - hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed hairpin.fa not found: ${references_parsed}"} - - mature = file("$params.references_parsed/mature.fa", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed mature.fa not found: ${references_parsed}"} - } else{ if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } - if (params.refgenome) {reference_genome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } } @@ -829,7 +818,7 @@ if (! params.skip_mirdeep){ publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' input: - file refgenome from reference_genome + file refgenome from refgenome file reads_collapsed from mirdeep_reads_collapsed file reads_vs_refdb from reads_vs_refdb file mature from mature From a9ff13c220ad7406ba2558e0001bc71ad1898af6 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?lluc=20cab=C3=BAs?= Date: Tue, 6 Oct 2020 08:57:07 +0200 Subject: [PATCH 036/146] changed skip_mirdeep --- conf/base.config | 8 ++++---- main.nf | 14 +++++--------- 2 files changed, 9 insertions(+), 13 deletions(-) diff --git a/conf/base.config b/conf/base.config index a6ed1a64..2668dfce 100644 --- a/conf/base.config +++ b/conf/base.config @@ -22,17 +22,17 @@ process { // Resource requirements withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 16.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_medium { cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 32.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 8 * task.attempt, 'cpus' ) } - memory = { check_max( 64.GB * task.attempt, 'memory' ) } + cpus = { check_max( 4 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } } diff --git a/main.nf b/main.nf index 1c7c23f8..431bf59d 100644 --- a/main.nf +++ b/main.nf @@ -148,10 +148,7 @@ else{ if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } if (params.refgenome) {reference_genome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } - } - - } if( params.bt_index ){ @@ -306,15 +303,15 @@ process get_software_versions { """ } - -if (! params.references_parsed & !params.skip_mirdeep){ +if (!params.references_parsed && !params.skip_mirdeep){ /* * PREPROCESSING - Parse genome.fa and build Bowtie index for refgenome */ process bowtie_indices { label 'process_medium' - publishDir "${params.outdir}/references", mode: 'symlink' - + publishDir path: { params.saveReference ? "${params.outdir}/references_parsed" : params.outdir }, + saveAs: { params.saveReference ? it : null }, mode: 'copy' + input: file refgenome from reference_genome file mature from reference_mature @@ -333,8 +330,7 @@ if (! params.references_parsed & !params.skip_mirdeep){ sed -i 's, ,_,g' $mature bowtie-build $refgenome genome """ - - } + } } /* * PREPROCESSING - Build Bowtie index for mature and hairpin From e92e8809bf2b4509d98369da89e6f9c6855cf1ca Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Tue, 6 Oct 2020 09:01:17 +0200 Subject: [PATCH 037/146] Update base.config --- conf/base.config | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/base.config b/conf/base.config index 2668dfce..a6ed1a64 100644 --- a/conf/base.config +++ b/conf/base.config @@ -22,17 +22,17 @@ process { // Resource requirements withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + memory = { check_max( 16.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_medium { cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + memory = { check_max( 32.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 4 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + cpus = { check_max( 8 * task.attempt, 'cpus' ) } + memory = { check_max( 64.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } } From ae718f952a6b8cd3d71f67c996eb455f8cfe786b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?lluc=20cab=C3=BAs?= Date: Tue, 6 Oct 2020 11:26:09 +0200 Subject: [PATCH 038/146] changed skip_mirdeep --- main.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/main.nf b/main.nf index 431bf59d..eab4ab85 100644 --- a/main.nf +++ b/main.nf @@ -126,10 +126,6 @@ if (!params.mirna_gtf && params.mirtrace_species){ // Validate inputs -if( !params.mature || !params.hairpin ){ - exit 1, "Missing mature / hairpin reference indexes! Is --genome specified?" -} - if (params.skip_mirdeep){ if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } @@ -151,6 +147,10 @@ else{ } } +if( !params.mature || !params.hairpin ){ + exit 1, "Missing mature / hairpin reference indexes! Is --genome specified?" +} + if( params.bt_index ){ bt_indices = Channel .fromPath("${params.bt_index}*", checkIfExists: true) @@ -311,7 +311,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ label 'process_medium' publishDir path: { params.saveReference ? "${params.outdir}/references_parsed" : params.outdir }, saveAs: { params.saveReference ? it : null }, mode: 'copy' - + input: file refgenome from reference_genome file mature from reference_mature From 0ced1f2aa7bb5928d3ac6a3c234f7b0086413d16 Mon Sep 17 00:00:00 2001 From: EC2 Default User Date: Tue, 6 Oct 2020 10:31:25 +0000 Subject: [PATCH 039/146] updated skip_mirdeep --- main.nf | 4 ---- 1 file changed, 4 deletions(-) diff --git a/main.nf b/main.nf index eab4ab85..687441dd 100644 --- a/main.nf +++ b/main.nf @@ -147,10 +147,6 @@ else{ } } -if( !params.mature || !params.hairpin ){ - exit 1, "Missing mature / hairpin reference indexes! Is --genome specified?" -} - if( params.bt_index ){ bt_indices = Channel .fromPath("${params.bt_index}*", checkIfExists: true) From 03f4d268358a69e17002727535394e85465eb095 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Thu, 8 Oct 2020 14:51:45 +0200 Subject: [PATCH 040/146] Update output.md --- docs/output.md | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index e3ccb4d2..91196bf7 100644 --- a/docs/output.md +++ b/docs/output.md @@ -131,12 +131,11 @@ This is an example of the output we can get: * `mirtop_rawData.tsv`: File compatible with [isomiRs](http://lpantano.github.io/isomiRs/reference/IsomirDataSeqFromMirtop.html) Bioconductor package to perform isomiRs analysis. * `mirna.tsv`: tabular file with miRNA counts after summarizing unique isomiRs for each miRNA -##miRDeep2 +## miRDeep2 [miRDeep2] (https://www.mdc-berlin.de/content/mirdeep2-documentation) is used for the identification of novel and known miRNAs in deep sequencing data. **Output directory: `results/mirdeep2`** -* `mapper/sample_trimmed_collapsed.fa`: trimmed reads collapsed in order to reduce disk space. * `mapper/sample_reads_vs_refdb.arf`: file with the mapped reads to the genome. * `mirdeep/timestamp_sample.bed`: file with the known and novel miRNAs in bed format. * `mirdeep/timestamp_sample.csv`: file with an overview of all detected miRNAs (known and novel) in csv format. From f9f7ab7d02b7ac8837bfc1236b46e84ea714f164 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Thu, 8 Oct 2020 14:52:12 +0200 Subject: [PATCH 041/146] Update output.md --- docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index 91196bf7..26f752db 100644 --- a/docs/output.md +++ b/docs/output.md @@ -132,7 +132,7 @@ This is an example of the output we can get: * `mirna.tsv`: tabular file with miRNA counts after summarizing unique isomiRs for each miRNA ## miRDeep2 -[miRDeep2] (https://www.mdc-berlin.de/content/mirdeep2-documentation) is used for the identification of novel and known miRNAs in deep sequencing data. +[miRDeep2](https://www.mdc-berlin.de/content/mirdeep2-documentation) is used for the identification of novel and known miRNAs in deep sequencing data. **Output directory: `results/mirdeep2`** From 2fe9bd8b756c15f781ee1518ec187e0ab956e0bb Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Thu, 8 Oct 2020 16:33:50 +0200 Subject: [PATCH 042/146] Update output.md --- docs/output.md | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/docs/output.md b/docs/output.md index 26f752db..d8064f58 100644 --- a/docs/output.md +++ b/docs/output.md @@ -136,10 +136,12 @@ This is an example of the output we can get: **Output directory: `results/mirdeep2`** -* `mapper/sample_reads_vs_refdb.arf`: file with the mapped reads to the genome. -* `mirdeep/timestamp_sample.bed`: file with the known and novel miRNAs in bed format. -* `mirdeep/timestamp_sample.csv`: file with an overview of all detected miRNAs (known and novel) in csv format. -* `mirdeep/timestamp_sample.html`: file with an overview of all detected miRNAs (known and novel) in html format. +* `mirdeep/timestamp_sample.bed` + * File with the known and novel miRNAs in bed format. +* `mirdeep/timestamp_sample.csv` + * File with an overview of all detected miRNAs (known and novel) in csv format. +* `mirdeep/timestamp_sample.html` + * A HTML report with an overview of all detected miRNAs (known and novel) in html format. ## miRTrace [miRTrace](https://github.com/friedlanderlab/mirtrace) is a quality control specifically for small RNA sequencing data (smRNA-Seq). Each sample is characterized by profiling sequencing quality, read length, sequencing depth and miRNA complexity and also the amounts of miRNAs versus undesirable sequences (derived from tRNAs, rRNAs and sequencing artifacts). From 45cc8ce94c0873e104aec39849e2eceff67e854f Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 9 Oct 2020 14:55:41 +0200 Subject: [PATCH 043/146] Update test.config --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 1e0b638d..69bb054e 100644 --- a/conf/test.config +++ b/conf/test.config @@ -24,7 +24,7 @@ params { // Genome references - skip_mirdeep = false + skip_mirdeep = true bt_indices = [ 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', From ac81490ad32e400f301cceacbf4bcff222c5af0b Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 16 Oct 2020 13:05:35 +0200 Subject: [PATCH 044/146] Update help text in main.nf Co-authored-by: James A. Fellows Yates --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 687441dd..1cdc95bc 100644 --- a/main.nf +++ b/main.nf @@ -53,7 +53,7 @@ def helpMessage() { -name Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic --seq_center Text about sequencing center which will be added in the header of output bam files --maxMultiqcEmailFileSize Theshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - --skip_mirdeep If this parameter is specified, it stops the detection of novel miRNAs with miRDeep2. Default: false + --skip_mirdeep If this parameter is specified, it stops the detection of novel miRNAs with miRDeep2. Default: ${params.skip_mirdeep} AWSBatch options: --awsqueue The AWSBatch JobQueue that needs to be set when running on AWSBatch From 96842842c81c8e27640cdf7929fd7e37ddddb116 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 16 Oct 2020 13:15:32 +0200 Subject: [PATCH 045/146] Update igenomes.config --- conf/igenomes.config | 53 +++++++++++++++----------------------------- 1 file changed, 18 insertions(+), 35 deletions(-) diff --git a/conf/igenomes.config b/conf/igenomes.config index 000127b9..1be4cd81 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -14,61 +14,55 @@ mature = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "hsa" } 'GRCm38' { mature = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "mmu" } 'TAIR10' { mature = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ath" } 'UMD3.1' { mature = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "bta" } 'WBcel235' { mature = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "cel" } 'CanFam3.1' { mature = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "cfa" } 'GRCz10' { mature = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" mirtrace_species = "dre" } @@ -76,99 +70,88 @@ mature = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "dme" } 'EquCab2' { mature = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "eca" } 'Galgal4' { mature = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "gga" } 'Gm01' { mature = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "gma" } 'Mmul_1' { mature = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "mml" } 'IRGSP-1.0' { mature = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "osa" } 'CHIMP2.1.4' { mature = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ptr" } 'Rnor_6.0' { mature = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "rno" } 'Sbi1' { mature = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "sbi" } 'Sscrofa10.2' { mature = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "ssc" } 'AGPv3' { mature = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/SmallRNA/mature.fa" hairpin = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/SmallRNA/hairpin.fa" bowtie = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BowtieIndex/genome" - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta" + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - refgenome = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" mirtrace_species = "zma" } } From 04cc83583a97a79a873941ac6350f2f3f2325ddf Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 16 Oct 2020 13:22:49 +0200 Subject: [PATCH 046/146] Changed --refgenome for --fasta Changed --refgenome for the nf-core default --fasta --- main.nf | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/main.nf b/main.nf index 1cdc95bc..46894423 100644 --- a/main.nf +++ b/main.nf @@ -33,7 +33,7 @@ def helpMessage() { --mirna_gtf GFF/GTF file with coordinates positions of precursor and miRNAs. See: ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3 --bt_index Path to the bowtie 1 index files of the host reference genome. Optional. --mirtrace_species Species for miRTrace. Pre-defined when '--genome' is specified. (hsa, mmu ...) - --refgenome Path to the Fasta file of the reference genome. + --fasta Path to genome fasta file --references_parsed Path to the parsed reference folder containing the files for the miRDeep2. Trimming options @@ -83,7 +83,7 @@ params.bt_index = params.genome ? params.genomes[ params.genome ].bowtie ?: fals params.mature = params.genome ? params.genomes[ params.genome ].mature ?: false : false params.hairpin = params.genome ? params.genomes[ params.genome ].hairpin ?: false : false params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtrace_species ?: false : false -params.refgenome = params.genome ? params.genomes[ params.genome ].refgenome ?: false : false +params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false // Define regular variables so that they can be overwritten clip_R1 = params.clip_R1 @@ -133,7 +133,7 @@ if (params.skip_mirdeep){ } else{ if (params.references_parsed){ - refgenome = file("$params.references_parsed/genome.fa", checkIfExists: true) + fasta = file("$params.references_parsed/genome.fa", checkIfExists: true) hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) mature = file("$params.references_parsed/mature.fa", checkIfExists: true) indices_mirdeep2 = Channel @@ -143,7 +143,7 @@ else{ else{ if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } - if (params.refgenome) {reference_genome = file(params.refgenome, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.refgenome}" } + if (params.fasta) {reference_genome = file(params.fasta, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.fasta}" } } } @@ -213,7 +213,7 @@ summary["Trim 5' R1"] = clip_R1 summary["Trim 3' R1"] = three_prime_clip_R1 summary['miRBase mature'] = params.mature summary['miRBase hairpin'] = params.hairpin -summary['Reference Genome'] = params.refgenome +summary['Reference Genome'] = params.fasta if(params.bt_index) summary['Bowtie Index for Ref'] = params.bt_index summary['Save Reference'] = params.saveReference ? 'Yes' : 'No' summary['Protocol'] = params.protocol @@ -301,7 +301,7 @@ process get_software_versions { if (!params.references_parsed && !params.skip_mirdeep){ /* - * PREPROCESSING - Parse genome.fa and build Bowtie index for refgenome + * PREPROCESSING - Parse genome.fa and build Bowtie index for the reference genome */ process bowtie_indices { label 'process_medium' @@ -314,7 +314,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ file hairpin from reference_hairpin output: - file 'genome.fa' into refgenome + file 'genome.fa' into fasta file 'genome.*.ebwt' into indices_mirdeep2 file 'hairpin.fa' into hairpin file 'mature.fa' into mature @@ -810,7 +810,7 @@ if (! params.skip_mirdeep){ publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' input: - file refgenome from refgenome + file refgenome from fasta file reads_collapsed from mirdeep_reads_collapsed file reads_vs_refdb from reads_vs_refdb file mature from mature From 983f716b020b7523fa759105e9c67f8356e653d3 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 16 Oct 2020 13:40:17 +0200 Subject: [PATCH 047/146] Update main.nf --- main.nf | 231 +++++++++++++++++++++++++++++--------------------------- 1 file changed, 118 insertions(+), 113 deletions(-) diff --git a/main.nf b/main.nf index 46894423..5675eb26 100644 --- a/main.nf +++ b/main.nf @@ -743,133 +743,138 @@ if( params.bt_index ) { ch_genome_bam_flagstat_mqc = Channel.empty() } /* - * STEP 7.0, 7.1 and 7.2 if skip_mirdeep is not specified + * STEP 7.0 - unzip trim galore reads */ -if (! params.skip_mirdeep){ - /* - * STEP 7.0 - unzip trim galore reads - */ - process uncompress_trimmed_reads{ - label 'process_low' +process uncompress_trimmed_reads{ + label 'process_low' - input: - path reads from trimmed_zipped_reads_mirdeep2 + when: + !params.skip_mirdeep + + input: + path reads from trimmed_zipped_reads_mirdeep2 - output: - path "$unzip" into trimmed_reads_mirdeep2 + output: + path "$unzip" into trimmed_reads_mirdeep2 - script: - unzip = reads.toString() - '.gz' - """ - pigz -f -d -p $task.cpus $reads - """ + script: + unzip = reads.toString() - '.gz' + """ + pigz -f -d -p $task.cpus $reads + """ - } +} - /* - * STEP 7.1 - miRDeep2 mapper - */ - process mapper_mirdeep2 { - label 'process_medium' - tag "$reads" - publishDir "${params.outdir}/mirdeep2/mapper", mode: 'copy' - - input: - file reads from trimmed_reads_mirdeep2 - file indices from indices_mirdeep2.collect() - - - output: - file '*_collapsed.fa' into mirdeep_reads_collapsed - file '*reads_vs_refdb.arf' into reads_vs_refdb - - script: - index_base = indices.toString().tokenize(' ')[0].tokenize('.')[0] - - """ - mapper.pl \\ - $reads \\ - -e \\ - -h \\ - -i \\ - -j \\ - -m \\ - -p $index_base \\ - -s ${reads.baseName}_collapsed.fa \\ - -t ${reads.baseName}_reads_vs_refdb.arf \\ - -o 4 - """ - } +/* + * STEP 7.1 - miRDeep2 mapper + */ +process mapper_mirdeep2 { + label 'process_medium' + tag "$reads" + publishDir "${params.outdir}/mirdeep2/mapper", mode: 'copy' - /* - * STEP 7.2 - miRDeep2 novel mirnas - */ - process mirdeep2 { - label 'process_medium' - publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' - - input: - file refgenome from fasta - file reads_collapsed from mirdeep_reads_collapsed - file reads_vs_refdb from reads_vs_refdb - file mature from mature - file hairpin from hairpin - - output: - - file 'result*.{bed,csv,html}' - - - script: - - """ - miRDeep2.pl \\ - $reads_collapsed \\ - $refgenome \\ - $reads_vs_refdb \\ - $mature \\ - none \\ - $hairpin \\ - -d \\ - -z _${reads_collapsed.simpleName} - """ - } + when: + !params.skip_mirdeep + + input: + file reads from trimmed_reads_mirdeep2 + file indices from indices_mirdeep2.collect() + + + output: + file '*_collapsed.fa' into mirdeep_reads_collapsed + file '*reads_vs_refdb.arf' into reads_vs_refdb + + script: + index_base = indices.toString().tokenize(' ')[0].tokenize('.')[0] + + """ + mapper.pl \\ + $reads \\ + -e \\ + -h \\ + -i \\ + -j \\ + -m \\ + -p $index_base \\ + -s ${reads.baseName}_collapsed.fa \\ + -t ${reads.baseName}_reads_vs_refdb.arf \\ + -o 4 + """ } +/* + * STEP 7.2 - miRDeep2 novel mirnas + */ +process mirdeep2 { + label 'process_medium' + publishDir "${params.outdir}/mirdeep2/mirdeep", mode: 'copy' + + when: + !params.skip_mirdeep + + input: + file refgenome from fasta + file reads_collapsed from mirdeep_reads_collapsed + file reads_vs_refdb from reads_vs_refdb + file mature from mature + file hairpin from hairpin + + output: + + file 'result*.{bed,csv,html}' + + + script: + + """ + miRDeep2.pl \\ + $reads_collapsed \\ + $refgenome \\ + $reads_vs_refdb \\ + $mature \\ + none \\ + $hairpin \\ + -d \\ + -z _${reads_collapsed.simpleName} + """ +} + + /* * STEP 8 - miRTrace */ process mirtrace { - tag "$reads" - publishDir "${params.outdir}/miRTrace", mode: 'copy' - - input: - file reads from raw_reads_mirtrace.collect() - - output: - file '*mirtrace' into mirtrace_results - - script: - primer = (protocol=="cats") ? " " : " --adapter $three_prime_adapter " - """ - for i in $reads - do - path=\$(realpath \${i}) - prefix=\$(echo \${i} | sed -e 's/.gz//' -e 's/.fastq//' -e 's/.fq//' -e 's/_val_1//' -e 's/_trimmed//' -e 's/_R1//' -e 's/.R1//') - echo \$path","\$prefix - done > mirtrace_config - - mirtrace qc \\ - --species $params.mirtrace_species \\ - $primer \\ - --protocol $protocol \\ - --config mirtrace_config \\ - --write-fasta \\ - --output-dir mirtrace \\ - --force - """ - } + tag "$reads" + publishDir "${params.outdir}/miRTrace", mode: 'copy' + + input: + file reads from raw_reads_mirtrace.collect() + + output: + file '*mirtrace' into mirtrace_results + + script: + primer = (protocol=="cats") ? " " : " --adapter $three_prime_adapter " + """ + for i in $reads + do + path=\$(realpath \${i}) + prefix=\$(echo \${i} | sed -e 's/.gz//' -e 's/.fastq//' -e 's/.fq//' -e 's/_val_1//' -e 's/_trimmed//' -e 's/_R1//' -e 's/.R1//') + echo \$path","\$prefix + done > mirtrace_config + + mirtrace qc \\ + --species $params.mirtrace_species \\ + $primer \\ + --protocol $protocol \\ + --config mirtrace_config \\ + --write-fasta \\ + --output-dir mirtrace \\ + --force + """ +} /* From e0de7cf5b4433cd79b690d1cbaef360f93b66839 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Fri, 16 Oct 2020 17:00:18 +0200 Subject: [PATCH 048/146] Update nextflow.config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 6fce5a60..d96820d8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -49,7 +49,7 @@ params { // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev -process.container = 'lluccabus/smrnaseq' +process.container = 'nfcore/smrnaseq:dev' // Load base.config by default for all pipelines includeConfig 'conf/base.config' From 40ca29ed5b89f6aaa067598c4f4b3ffe301d537c Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:42:21 +0200 Subject: [PATCH 049/146] Update base.config --- conf/base.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/base.config b/conf/base.config index a6ed1a64..9f15b72c 100644 --- a/conf/base.config +++ b/conf/base.config @@ -12,7 +12,7 @@ process { cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + memory = { check_max( 8.GB * task.attempt, 'memory' ) } time = { check_max( 2.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } From edbdc4b68edc7ad1f2b1ec234ebea996b75964b9 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:44:29 +0200 Subject: [PATCH 050/146] Update base.config --- conf/base.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/base.config b/conf/base.config index 9f15b72c..c7ae2f9c 100644 --- a/conf/base.config +++ b/conf/base.config @@ -42,5 +42,5 @@ params { max_memory = 128.GB max_cpus = 16 max_time = 240.h - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' } From fa719205f11f21c94d68974c64188c045a977549 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:50:11 +0200 Subject: [PATCH 051/146] Update test.config --- conf/test.config | 18 +++++++----------- 1 file changed, 7 insertions(+), 11 deletions(-) diff --git a/conf/test.config b/conf/test.config index 69bb054e..bb453a57 100644 --- a/conf/test.config +++ b/conf/test.config @@ -15,28 +15,24 @@ params { max_memory = 6.GB max_time = 48.h // Input data - readPaths = [ 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' ] - - // Genome references - skip_mirdeep = true bt_indices = [ - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' ] gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' mirna_gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3' mirtrace_species = "hsa" - +skip_mirdeep = true } From 1ef2f6400e9e9be1d82e1e1658659881ac3ce571 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:50:36 +0200 Subject: [PATCH 052/146] Update test.config --- conf/test.config | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/conf/test.config b/conf/test.config index bb453a57..78a3a0d5 100644 --- a/conf/test.config +++ b/conf/test.config @@ -16,18 +16,18 @@ params { max_time = 48.h // Input data readPaths = [ - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' ] // Genome references bt_indices = [ - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', - 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.2.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.3.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', + 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' ] gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' From 6b9552cbe4a7b3d942f9bef0b82adffc5f456c4a Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:51:03 +0200 Subject: [PATCH 053/146] Update test.config --- conf/test.config | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/conf/test.config b/conf/test.config index 78a3a0d5..81ebc2c1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,10 +29,10 @@ params { 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' ] -gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' -mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' -hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' -mirna_gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3' -mirtrace_species = "hsa" -skip_mirdeep = true + gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' + mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' + hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' + mirna_gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hsa.gff3' + mirtrace_species = "hsa" + skip_mirdeep = true } From 1cfaefe95f509fc2055aadaede6323b24add6184 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Mon, 19 Oct 2020 12:51:31 +0200 Subject: [PATCH 054/146] Update test.config --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 81ebc2c1..ec5a5c98 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,7 +19,7 @@ params { 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_2.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/sample_3.fastq.gz' - ] + ] // Genome references bt_indices = [ 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.1.ebwt', From 2a79a3cc4eee24ae985f8de2b9088d89d85c2b82 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Wed, 21 Oct 2020 13:35:43 +0200 Subject: [PATCH 055/146] Update nextflow.config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index e618bfe0..d3051d2e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -54,7 +54,7 @@ params { // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev -process.container = 'lluccabus/smrnaseq' +process.container = 'nfcore/smrnaseq:dev' // Load base.config by default for all pipelines includeConfig 'conf/base.config' From 99a52e623be22d2311744707b42afbf6e55df357 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Wed, 21 Oct 2020 14:21:09 +0200 Subject: [PATCH 056/146] Update docs/output.md Co-authored-by: Maxime Garcia --- docs/output.md | 1 + 1 file changed, 1 insertion(+) diff --git a/docs/output.md b/docs/output.md index ea11bf33..dde144b7 100644 --- a/docs/output.md +++ b/docs/output.md @@ -139,6 +139,7 @@ This is an example of the output we can get: * `mirna.tsv`: tabular file with miRNA counts after summarizing unique isomiRs for each miRNA ## miRDeep2 + [miRDeep2](https://www.mdc-berlin.de/content/mirdeep2-documentation) is used for the identification of novel and known miRNAs in deep sequencing data. **Output directory: `results/mirdeep2`** From e1d96bad562a7cf25a494f5668e1ac73b5b5860e Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Wed, 21 Oct 2020 14:23:12 +0200 Subject: [PATCH 057/146] Delete awsbatch.config --- conf/awsbatch.config | 18 ------------------ 1 file changed, 18 deletions(-) delete mode 100644 conf/awsbatch.config diff --git a/conf/awsbatch.config b/conf/awsbatch.config deleted file mode 100644 index f14e0a1b..00000000 --- a/conf/awsbatch.config +++ /dev/null @@ -1,18 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for running on AWS batch - * ------------------------------------------------- - * Base config needed for running with -profile awsbatch - */ -params { - config_profile_name = 'AWSBATCH' - config_profile_description = 'AWSBATCH Cloud Profile' - config_profile_contact = 'Alexander Peltzer (@apeltzer)' - config_profile_url = 'https://aws.amazon.com/de/batch/' -} - -aws.region = params.awsregion -process.executor = 'awsbatch' -process.queue = params.awsqueue -aws.batch.cliPath = '/home/ec2-user/miniconda/bin/aws' -params.tracedir = './' From 6730677dc371be90be638ad8a3676a25307888a7 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 27 Oct 2020 09:25:30 +0000 Subject: [PATCH 058/146] Template update for nf-core/tools version 1.11 --- .github/CONTRIBUTING.md | 2 +- .github/ISSUE_TEMPLATE/bug_report.md | 2 +- .github/workflows/awsfulltest.yml | 4 +++- .github/workflows/awstest.yml | 9 ++++----- Dockerfile | 2 +- README.md | 10 +++++----- docs/README.md | 2 -- docs/output.md | 8 +++++++- docs/usage.md | 13 ++++++++++--- nextflow.config | 3 +++ nextflow_schema.json | 4 ++-- 11 files changed, 37 insertions(+), 22 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 36dae17b..d0561a2a 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -54,4 +54,4 @@ These tests are run both with the latest available version of `Nextflow` and als ## Getting help -For further information/help, please consult the [nf-core/smrnaseq documentation](https://nf-co.re/smrnaseq/docs) and don't hesitate to get in touch on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). +For further information/help, please consult the [nf-core/smrnaseq documentation](https://nf-co.re/smrnaseq/usage) and don't hesitate to get in touch on the nf-core Slack [#smrnaseq](https://nfcore.slack.com/channels/smrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index fb773235..1c58337b 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -36,7 +36,7 @@ Steps to reproduce the behaviour: ## Container engine -- Engine: +- Engine: - version: - Image tag: diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 7d6e077f..84250fbe 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -1,10 +1,12 @@ name: nf-core AWS full size tests -# This workflow is triggered on push to the master branch. +# This workflow is triggered on published releases. +# It can be additionally triggered manually with GitHub actions workflow dispatch. # It runs the -profile 'test_full' on AWS batch on: release: types: [published] + workflow_dispatch: jobs: run-awstest: diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index bba984a5..5e62f62f 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -1,11 +1,10 @@ name: nf-core AWS test # This workflow is triggered on push to the master branch. -# It runs the -profile 'test' on AWS batch +# It can be additionally triggered manually with GitHub actions workflow dispatch. +# It runs the -profile 'test' on AWS batch. on: - push: - branches: - - master + workflow_dispatch: jobs: run-awstest: @@ -37,4 +36,4 @@ jobs: --job-name nf-core-smrnaseq \ --job-queue $AWS_JOB_QUEUE \ --job-definition $AWS_JOB_DEFINITION \ - --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}'smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' + --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' diff --git a/Dockerfile b/Dockerfile index 641eadc7..1618e35a 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.10.2 +FROM nfcore/base:1.11 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" diff --git a/README.md b/README.md index 2652607e..958835d8 100644 --- a/README.md +++ b/README.md @@ -18,12 +18,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool 1. Install [`nextflow`](https://nf-co.re/usage/installation) -2. Install either [`Docker`](https://docs.docker.com/engine/installation/) or [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ +2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`Podman`](https://podman.io/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ 3. Download the pipeline and test it on a minimal dataset with a single command: ```bash - nextflow run nf-core/smrnaseq -profile test, + nextflow run nf-core/smrnaseq -profile test, ``` > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. @@ -33,14 +33,14 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ```bash - nextflow run nf-core/smrnaseq -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + nextflow run nf-core/smrnaseq -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 ``` -See [usage docs](docs/usage.md) for all of the available options when running the pipeline. +See [usage docs](https://nf-co.re/smrnaseq/usage) for all of the available options when running the pipeline. ## Documentation -The nf-core/smrnaseq pipeline comes with documentation about the pipeline which you can read at [https://nf-core/smrnaseq/docs](https://nf-core/smrnaseq/docs) or find in the [`docs/` directory](docs). +The nf-core/smrnaseq pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/smrnaseq/usage) and [output](https://nf-co.re/smrnaseq/output). diff --git a/docs/README.md b/docs/README.md index 575abde1..6ea17c7e 100644 --- a/docs/README.md +++ b/docs/README.md @@ -2,8 +2,6 @@ The nf-core/smrnaseq documentation is split into the following pages: - - * [Usage](usage.md) * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. * [Output](output.md) diff --git a/docs/output.md b/docs/output.md index 0b4b8cc4..9232e4cf 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,5 +1,11 @@ # nf-core/smrnaseq: Output +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/smrnaseq/output](https://nf-co.re/smrnaseq/output) + +> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ + +## Introduction + This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -40,7 +46,7 @@ For more information about how to use MultiQC reports, see [https://multiqc.info **Output files:** -* `multiqc/` +* `multiqc/` * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. * `multiqc_plots/`: directory containing static images from the report in various formats. diff --git a/docs/usage.md b/docs/usage.md index 7489f0b7..c5e9d84d 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,5 +1,9 @@ # nf-core/smrnaseq: Usage +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/smrnaseq/usage](https://nf-co.re/smrnaseq/usage) + +> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ + ## Introduction @@ -47,7 +51,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below. > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -64,8 +68,11 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * `singularity` * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) +* `podman` + * A generic configuration profile to be used with [Podman](https://podman.io/) + * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) * `conda` - * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker or Singularity. + * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman. * A generic configuration profile to be used with [Conda](https://conda.io/docs/) * Pulls most software from [Bioconda](https://bioconda.github.io/) * `test` @@ -98,7 +105,7 @@ process { See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. -If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. +If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). diff --git a/nextflow.config b/nextflow.config index bd08e4d9..a0df5667 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,6 +71,9 @@ profiles { singularity.enabled = true singularity.autoMounts = true } + podman { + podman.enabled = true + } test { includeConfig 'conf/test.config' } } diff --git a/nextflow_schema.json b/nextflow_schema.json index f5ee5c6f..55101fc8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,5 +1,5 @@ { - "$schema": "https://json-schema.org/draft-07/schema", + "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/nf-core/smrnaseq/master/nextflow_schema.json", "title": "nf-core/smrnaseq pipeline parameters", "description": "Small RNA-Seq Best Practice Analysis Pipeline.", @@ -101,7 +101,7 @@ "link", "copy", "copyNoFollow", - "mov" + "move" ] }, "name": { From 3e85b4c675c2144d9c4125dff5dd6d91624eb306 Mon Sep 17 00:00:00 2001 From: lcabus-flomics <61687053+lcabus-flomics@users.noreply.github.com> Date: Wed, 4 Nov 2020 17:09:30 +0100 Subject: [PATCH 059/146] Update main.nf --- main.nf | 3 --- 1 file changed, 3 deletions(-) diff --git a/main.nf b/main.nf index 87b50685..32cbd522 100644 --- a/main.nf +++ b/main.nf @@ -38,9 +38,6 @@ def helpMessage() { --mirtrace_species [str] Species for miRTrace. Pre-defined when '--genome' is specified. (hsa, mmu ...) --references_parsed [folder] Path to the parsed reference folder containing the files for the miRDeep2 ->>>>>>> origin/dev - - Trimming options --three_prime_adapter [str] 3’ Adapter to trim. Default: None --min_length [int] Discard reads that became shorter than length [int] because of either quality or adapter trimming. Default: 18 From d087985926f6bb57030e2a73a1fb2c7c5476b30b Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Fri, 6 Nov 2020 23:25:14 +0100 Subject: [PATCH 060/146] Remove very old installation docs --- docs/installation.md | 144 ------------------------------------------- 1 file changed, 144 deletions(-) delete mode 100644 docs/installation.md diff --git a/docs/installation.md b/docs/installation.md deleted file mode 100644 index fdd10603..00000000 --- a/docs/installation.md +++ /dev/null @@ -1,144 +0,0 @@ -# nf-core/smrnaseq installation - -To start using the nf-core/smrnaseq pipeline, follow the steps below: - -1. [Install Nextflow](#1-install-nextflow) -2. [Install the pipeline](#2-install-the-pipeline) - * [Automatic](#21-automatic) - * [Offline](#22-offline) - * [Development](#23-development) -3. [Pipeline configuration](#3-pipeline-configuration) - * [Software deps: Docker and Singularity](#31-software-deps-docker-and-singularity) - * [Software deps: Bioconda](#32-software-deps-bioconda) - * [Configuration profiles](#33-configuration-profiles) -4. [Reference genomes](#4-reference-genomes) -5. [Appendices](#appendices) - * [Running on UPPMAX](#running-on-uppmax) - -## 1) Install NextFlow -Nextflow runs on most POSIX systems (Linux, Mac OSX etc). It can be installed by running the following commands: - -```bash -# Make sure that Java v8+ is installed: -java -version - -# Install Nextflow -curl -fsSL get.nextflow.io | bash - -# Add Nextflow binary to your PATH: -mv nextflow ~/bin -# OR system-wide installation: -# sudo mv nextflow /usr/local/bin -``` - -See [nextflow.io](https://www.nextflow.io/) for further instructions on how to install and configure Nextflow. - -## 2) Install the pipeline - -#### 2.1) Automatic -This pipeline itself needs no installation - NextFlow will automatically fetch it from GitHub if `nf-core/smrnaseq` is specified as the pipeline name. - -#### 2.2) Offline -The above method requires an internet connection so that Nextflow can download the pipeline files. If you're running on a system that has no internet connection, you'll need to download and transfer the pipeline files manually: - -```bash -# Download the latest release of the pipeline (see https://github.com/nf-core/smrnaseq/releases) -curl -L https://github.com/nf-core/smrnaseq/archive/1.0.zip -o nf-core-smrnaseq-v1.0.zip -unzip nf-core-smrnaseq-v1.0.zip -cd /path/to/my/data -nextflow run /path/to/pipelines/nf-core-smrnaseq-v1.0 [parameters] -``` - -#### 2.3) Development - -If you would like to make changes to the pipeline, it's best to make a fork on GitHub and then clone the files. Once cloned you can run the pipeline directly as above. - - -## 3) Pipeline configuration -By default, the pipeline runs with the `standard` configuration profile. This uses a number of sensible defaults for process requirements and is suitable for running on a simple (if powerful!) basic server. You can see this configuration in [`conf/base.config`](../conf/base.config). - -Be warned of two important points about this default configuration: - -1. The default profile uses the `local` executor - * All jobs are run in the login session. If you're using a simple server, this may be fine. If you're using a compute cluster, this is bad as all jobs will run on the head node. - * See the [nextflow docs](https://www.nextflow.io/docs/latest/executor.html) for information about running with other hardware backends. Most job scheduler systems are natively supported. -2. Nextflow will expect all software to be installed and available on the `PATH` - -#### 3.1) Software deps: Docker and Singularity -Running the pipeline with the option `-with-singularity` or `-with-docker` tells Nextflow to enable either [Singularity](http://singularity.lbl.gov/) or Docker for this run. An image containing all of the software requirements will be automatically fetched and used (https://hub.docker.com/r/nf-core/smrnaseq). - -If running offline with Singularity, you'll need to download and transfer the Singularity image first: - -```bash -singularity pull --name nfcore-smrnaseq-[VERSION].simg shub://nfcore/smrnaseq:[VERSION] -``` - -Once transferred, use `-with-singularity` but specify the path to the image file: - -```bash -nextflow run /path/to/nf-core-smrnaseq -with-singularity /path/to/nfcore-smrnaseq-[VERSION].simg -``` - -#### 3.2) Software deps: bioconda - -If you're unable to use either Docker or Singularity but you have conda installed, you can use the bioconda environment that comes with the pipeline. Running this command will create a new conda environment with all of the required software installed: - -```bash -conda env create -f environment.yml -conda clean -a # Recommended, not essential -source activate nfcore-smrnaseq-1.3 # Name depends on version -``` - -The [`environment.yml`](../environment.yml) file is packaged with the pipeline. Note that you may need to download this file from the [GitHub project page](https://github.com/nf-core/smrnaseq) if nextflow is automatically fetching the pipeline files. Ensure that the bioconda environment file version matches the pipeline version that you run. - - -#### 3.3) Configuration profiles - -Nextflow can be configured to run on a wide range of different computational infrastructures. In addition to the above pipeline-specific parameters it is likely that you will need to define system-specific options. For more information, please see the [Nextflow documentation](https://www.nextflow.io/docs/latest/). - -Whilst most parameters can be specified on the command line, it is usually sensible to create a configuration file for your environment. - -If you are the only person to be running this pipeline, you can create your config file as `~/.nextflow/config` and it will be applied every time you run Nextflow. Alternatively, save the file anywhere and reference it when running the pipeline with `-c path/to/config`. - -If you think that there are other people using the pipeline who would benefit from your configuration (e.g. other common cluster setups), please let us know. We can add a new configuration and profile which can used by specifying `-profile ` when running the pipeline. - -The pipeline comes with several such config profiles - see the installation appendices and usage documentation for more information. - -## 4) Reference Genomes -The nf-core/smrnaseq pipeline needs a reference genome for read alignment. Support for many common genomes is built in if running on UPPMAX or AWS, by using [AWS-iGenomes](https://ewels.github.io/AWS-iGenomes/). - -Alternatively, you can supply either a Bismark / BWA reference or a FASTA file. This can be done on the command line (see the [usage docs](usage.md#supplying-reference-indices)). -You can use the same pipeline config framework as used with iGenomes to specify multiple references of your own. Add the paths to your NextFlow config under a relevant id and just specify this id with `--genome ID` when you run the pipeline: - -```nextflow -params { - genomes { - 'YOUR-ID' { - bismark = '/BismarkIndex' - bwa_meth = '/genome.fa' - fasta = '/genome.fa' // used if above is not specified - } - 'OTHER-GENOME' { - // [..] - } - } - // Optional - default genome. Ignored if --genome 'OTHER-GENOME' specified on command line - genome = 'YOUR-ID' -} -``` - - -## Appendices - -#### Running on UPPMAX -To run the pipeline on the [Swedish UPPMAX](https://www.uppmax.uu.se/) clusters (`rackham`, `irma`, `bianca` etc), use the command line flag `-profile uppmax`. This tells Nextflow to submit jobs using the SLURM job executor with Singularity for software dependencies. - -Note that you will need to specify your UPPMAX project ID when running a pipeline. To do this, use the command line flag `--project `. The pipeline will exit with an error message if you try to run it pipeline with the default UPPMAX config profile without a project. - -**Optional Extra:** To avoid having to specify your project every time you run Nextflow, you can add it to your personal Nextflow config file instead. Add this line to `~/.nextflow/config`: - -```nextflow -params.project = 'project_ID' // eg. b2017123 -``` - -Bianca users - see the above docs about using the pipeline and singularity containers offline. From 5adba2a5544e44291477f1b51c4ac64866561e34 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Fri, 6 Nov 2020 23:42:48 +0100 Subject: [PATCH 061/146] Replace --reads with --input --- CHANGELOG.md | 3 ++- docs/usage.md | 6 +++--- main.nf | 10 +++++----- nextflow.config | 4 ++-- 4 files changed, 12 insertions(+), 11 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ed7e8c4d..84331f19 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,8 +3,9 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0.1dev - [date] +## v1.1dev - [date] +* Change `--reads` to `--input` for consistency with rest of nf-core * Update with the latest TEMPLATE version and nf-core 1.11 * Update conda environment with new packages and updates diff --git a/docs/usage.md b/docs/usage.md index a3d34aa9..f25c00d1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -11,7 +11,7 @@ The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/smrnaseq --reads '*.fastq.gz' -profile docker +nextflow run nf-core/smrnaseq --input '*.fastq.gz' -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -77,12 +77,12 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * A profile with a complete configuration for automated testing * Includes links to test data so needs no other parameters -### `--reads` +### `--input` Use this to specify the location of your input FastQ files. For example: ```bash - --reads 'path/to/data/*.fastq.gz' + --input 'path/to/data/*.fastq.gz' ``` Please note the following requirements: diff --git a/main.nf b/main.nf index dc5c8672..586f042a 100644 --- a/main.nf +++ b/main.nf @@ -17,10 +17,10 @@ def helpMessage() { The typical command for running the pipeline is as follows: - nextflow run nf-core/smrnaseq --reads '*.fastq.gz' --genome GRCh37 -profile docker + nextflow run nf-core/smrnaseq --input '*.fastq.gz' --genome GRCh37 -profile docker Mandatory arguments: - --reads [file] Path to input data (must be surrounded with quotes) + --input [file] Path to input data (must be surrounded with quotes) -profile [str] Configuration profile to use. Can use multiple (comma separated) Available: conda, docker, singularity, test, awsbatch, and more --protocol [str] Library preparation protocol. Default: "illumina". Can be set as "illumina", "nextflex", "qiaseq" or "cats" @@ -198,8 +198,8 @@ if(params.input_paths){ .into { raw_reads_fastqc; raw_reads_trimgalore; raw_reads_mirtrace } } else { Channel - .fromPath( params.reads ) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.reads}" } + .fromPath( params.input ) + .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}" } .into { raw_reads_fastqc; raw_reads_trimgalore; raw_reads_mirtrace } } @@ -208,7 +208,7 @@ log.info nfcoreHeader() def summary = [:] if(workflow.revision) summary['Pipeline Release'] = workflow.revision summary['Run Name'] = custom_runName ?: workflow.runName -summary['Reads'] = params.reads +summary['Input'] = params.input summary['Genome'] = params.genome summary['Min Trimmed Length'] = params.min_length summary["Trim 5' R1"] = clip_R1 diff --git a/nextflow.config b/nextflow.config index 194daa4c..de0c6c7b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,7 +9,7 @@ params { // Workflow flags - reads = "data/*.fastq.gz" + input = "data/*.fastq.gz" outdir = './results' protocol = 'illumina' genome = false @@ -127,7 +127,7 @@ manifest { description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' nextflowVersion = '>=19.10.0' - version = '1.0.1dev' + version = '1.1dev' } // Function to ensure that resource requirements don't go beyond From 8fe4ed4269abb30eba88c31d3f554b3c7d1da8a8 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Fri, 6 Nov 2020 23:43:30 +0100 Subject: [PATCH 062/146] Remove params.single_end --- conf/test_full.config | 1 - nextflow.config | 1 - nextflow_schema.json | 6 ------ 3 files changed, 8 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index f06cc1e2..8461afe9 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,7 +14,6 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false input_paths = [ ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] diff --git a/nextflow.config b/nextflow.config index de0c6c7b..5248d5ba 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,7 +13,6 @@ params { outdir = './results' protocol = 'illumina' genome = false - single_end = true clip_R1 = 0 three_prime_clip_R1 = 0 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" diff --git a/nextflow_schema.json b/nextflow_schema.json index 55101fc8..cc783708 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -20,12 +20,6 @@ "description": "Input FastQ files.", "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" }, - "single_end": { - "type": "boolean", - "description": "Specifies that the input is single-end reads.", - "fa_icon": "fas fa-align-center", - "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run." - }, "outdir": { "type": "string", "description": "The output directory where the results will be saved.", From 9c9db1c7d8119c5e6e784641b8c2a0ffb6bbcd4c Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Fri, 6 Nov 2020 23:45:47 +0100 Subject: [PATCH 063/146] Bump version to 1.1dev and add skeleton schema --- Dockerfile | 4 +-- environment.yml | 2 +- nextflow_schema.json | 60 ++++++++++++++++++++++++++++++++++++++++++-- 3 files changed, 61 insertions(+), 5 deletions(-) diff --git a/Dockerfile b/Dockerfile index 1618e35a..936dfeaf 100644 --- a/Dockerfile +++ b/Dockerfile @@ -7,10 +7,10 @@ COPY environment.yml / RUN conda env create --quiet -f /environment.yml && conda clean -a # Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.0.1dev/bin:$PATH +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1dev/bin:$PATH # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-smrnaseq-1.0.1dev > nf-core-smrnaseq-1.0.1dev.yml +RUN conda env export --name nf-core-smrnaseq-1.1dev > nf-core-smrnaseq-1.1dev.yml # Instruct R processes to use these empty files instead of clashing with a local version RUN touch .Rprofile diff --git a/environment.yml b/environment.yml index ceebfc7d..4a09097d 100644 --- a/environment.yml +++ b/environment.yml @@ -1,6 +1,6 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.0.1dev +name: nf-core-smrnaseq-1.1dev channels: - conda-forge - bioconda diff --git a/nextflow_schema.json b/nextflow_schema.json index cc783708..af29b6da 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -249,5 +249,61 @@ { "$ref": "#/definitions/institutional_config_options" } - ] -} + ], + "properties": { + "protocol": { + "type": "string", + "default": "illumina" + }, + "clip_R1": { + "type": "integer", + "default": 0 + }, + "three_prime_clip_R1": { + "type": "integer", + "default": 0 + }, + "three_prime_adapter": { + "type": "string", + "default": "TGGAATTCTCGGGTGCCAAGG" + }, + "min_length": { + "type": "integer", + "default": 17 + }, + "skipQC": { + "type": "string" + }, + "skipFastqc": { + "type": "string" + }, + "skipMultiqc": { + "type": "string" + }, + "skip_mirdeep": { + "type": "string" + }, + "saveReference": { + "type": "string", + "default": "true" + }, + "seq_center": { + "type": "string" + }, + "references_parsed": { + "type": "string" + }, + "bt_index": { + "type": "string" + }, + "mature": { + "type": "string" + }, + "hairpin": { + "type": "string" + }, + "mirtrace_species": { + "type": "string" + } + } +} \ No newline at end of file From 087cceaf0cc33fb6bf40e1a08880344e609cb661 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Sat, 7 Nov 2020 00:44:37 +0100 Subject: [PATCH 064/146] Moved parameter documentation from usage.md to pipeline schema. --- CHANGELOG.md | 1 + docs/usage.md | 218 ++++--------------------------------------- nextflow_schema.json | 177 +++++++++++++++++++++++------------ 3 files changed, 136 insertions(+), 260 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 84331f19..d0788018 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,6 +6,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v1.1dev - [date] * Change `--reads` to `--input` for consistency with rest of nf-core +* Move all parameter documentation into new `nextflow_schema.json` file. * Update with the latest TEMPLATE version and nf-core 1.11 * Update conda environment with new packages and updates diff --git a/docs/usage.md b/docs/usage.md index f25c00d1..d06a49fc 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -41,6 +41,13 @@ First, go to the [nf-core/smrnaseq releases page](https://github.com/nf-core/smr This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. +## Stand-alone scripts + +The `bin` directory contains some scripts used by the pipeline which may also be run manually: + +* `edgeR_miRBase.r` + * R script using for processing reads counts of mature miRNAs and miRNA precursors (hairpins). + ## Core Nextflow arguments > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). @@ -77,34 +84,7 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * A profile with a complete configuration for automated testing * Includes links to test data so needs no other parameters -### `--input` - -Use this to specify the location of your input FastQ files. For example: - -```bash - --input 'path/to/data/*.fastq.gz' -``` - -Please note the following requirements: - -1. The path must be enclosed in quotes -2. The path must have at least one `*` wildcard character - -### `--protocol` - -Protocol for constructing smRNA-seq libraries. Note that trimming parameters and 3' adapter sequence are pre-defined with a specified protocol. -Default: "illumina" - -```bash ---protocol [one protocol listed in the table below] -``` - -| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence | -| :------------ | :-------------------------------------- | :----------------------------------- | :------------------- | -| illumina | Illumina TruSeq Small RNA | clip_R1 = 0; three_prime_clip_R1 = 0 | TGGAATTCTCGGGTGCCAAGG | -| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | clip_R1 = 4; three_prime_clip_R1 = 4 | TGGAATTCTCGGGTGCCAAGG | -| qiaseq | QIAGEN QIAseq miRNA | clip_R1 = 0; three_prime_clip_R1 = 0 | AACTGTAGGCACCATCAAT | -| cats | Diagenode CATS Small RNA-seq | clip_R1 = 3; three_prime_clip_R1 = 0 | AAAAAAAAAAA + GATCGGAAGAGCACACGTCTG (only polyA is used for trimming) | +### `-resume` Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. @@ -118,112 +98,17 @@ Specify the path to a specific config file (this is a core Nextflow command). Se Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. +Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config: + ```nextflow -params { - genomes { - 'GRCh37' { - fasta = '' // Used if no star index given - mature = '' //mature.fa" - hairpin = '' //hairpin.fa" - bowtie = '' // index - gtf = '' - mirtrace_species = "sps" // species according mirbase - - } - // Any number of additional genomes, key is used with --genome +process { + withName: star { + memory = 32.GB } } ``` -### `--saveReference` - -Supply this parameter to save any generated reference genome files to your results folder. These can then be used for future pipeline runs, reducing processing times. - -### `--fasta` - -If you prefer, you can specify the full path to your reference genome when you run the pipeline: - -```bash ---fasta '[path to Fasta reference]' -``` - -### `--igenomes_ignore` - -Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`. - -### `--mature` - -If you prefer, you can specify the full path to the FASTA file of mature miRNAs when you run the pipeline: - -```bash ---mature [path to the FASTA file of mature miRNAs] -``` - -### `--hairpin` - -If you prefer, you can specify the full path to the FASTA file of miRNA precursors when you run the pipeline: - -```bash ---hairpin [path to the FASTA file of miRNA precursors] -``` - -### `--bt_index` - -If you prefer, you can specify the full path to your reference genome when you run the pipeline: - -```bash ---bt_index [path to Bowtie 1 index] -``` - -## Trimming options - -### `--min_length [int]` - -Discard reads that became shorter than length [int] because of either quality or adapter trimming. Default: 18 - -### `--clip_R1 [int]` - -Instructs Trim Galore to remove bp from the 5' end of read 1 - -### `--three_prime_clip_R1 [int]` - -Instructs Trim Galore to remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed - -### `--three_prime_adapter [sequence]` - -Instructs Trim Galore to remove 3' adapters which are typically used in smRNA-seq library preparation - -## Skipping QC steps - -### `--skipQC` - -Skip all QC steps aside from MultiQC - -### `--skipFastqc` - -Skip FastQC - -### `--skipMultiqc` - -Skip MultiQC - -## Job resources - -### Automatic resubmission - -Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. - -### Custom resource requests - -Wherever process-specific requirements are set in the pipeline, the default value can be changed by creating a custom config file. See the files hosted at [`nf-core/configs`](https://github.com/nf-core/configs/tree/master/conf) for examples. - -If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition below). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. - -If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack). - -## AWS Batch specific parameters - -Running the pipeline on AWS Batch requires a couple of specific parameters to be set according to your AWS Batch configuration. Please use [`-profile awsbatch`](https://github.com/nf-core/configs/blob/master/conf/awsbatch.config) and then specify all of the following parameters +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. @@ -240,79 +125,8 @@ Some HPC setups also allow you to run nextflow within a cluster job submitted yo #### Nextflow memory requirements -## Other command line parameters - -### `--outdir` - -The output directory where the results will be saved. - -### `--email` - -Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run. - -### `--email_on_fail` - -This works exactly as with `--email`, except emails are only sent if the workflow is not successful. - -### `--max_multiqc_email_size` - -Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB). - -### `-name` - -Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic. - -This is used in the MultiQC report (if not default) and in the summary HTML / e-mail (always). - -**NB:** Single hyphen (core Nextflow option) - -### `--seq_center` - -Text about sequencing center which will be added in the header of output bam files. - -### `-resume` - -Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. - -You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. - -**NB:** Single hyphen (core Nextflow option) - -### `-c` - -Specify the path to a specific config file (this is a core NextFlow command). - -**NB:** Single hyphen (core Nextflow option) - -Note - you can use this to override defaults. For example, we run on UPPMAX but don't want to use the MultiQC -environment module as is the default. So we specify a config file using `-c` that contains the following: - -```nextflow -process.$multiqc.module = [] -``` - -## Stand-alone scripts - -The `bin` directory contains some scripts used by the pipeline which may also be run manually: - -* `edgeR_miRBase.r` - * R script using for processing reads counts of mature miRNAs and miRNA precursors (hairpins). - -### `--custom_config_version` - -Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`. - -```bash -## Download and use config file with following git commid id ---custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96 -``` - -### `--custom_config_base` - -If you're running offline, nextflow will not be able to fetch the institutional config files -from the internet. If you don't need them, then this is not a problem. If you do need them, -you should download the files from the repo and tell nextflow where to find them with the -`custom_config_base` option. For example: +In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. +We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): ```bash NXF_OPTS='-Xms1g -Xmx4g' diff --git a/nextflow_schema.json b/nextflow_schema.json index af29b6da..86c2637e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -18,7 +18,20 @@ "type": "string", "fa_icon": "fas fa-dna", "description": "Input FastQ files.", - "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" + "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n\n> NB: **This pipeline does not support paired-end data.**\n> If you have paired-end data, please just pass the _Read 1_ files to the pipeline." + }, + "protocol": { + "type": "string", + "default": "illumina", + "fa_icon": "fas fa-vial", + "description": "Protocol for constructing smRNA-seq libraries.", + "help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_R1 = 0` `three_prime_clip_R1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_R1 = 4` `three_prime_clip_R1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_R1 = 0` `three_prime_clip_R1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_R1 = 3` `three_prime_clip_R1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |", + "enum": [ + "illumina", + "nextflex", + "qiaseq", + "cats" + ] }, "outdir": { "type": "string", @@ -47,12 +60,45 @@ "fa_icon": "fas fa-book", "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." }, + "mirtrace_species": { + "type": "string", + "description": "Species for miRTrace.", + "help_text": "This is automatically set when using `--genome`. Example values: `hsa`, `mmu`...", + "fa_icon": "fas fa-journal-whills" + }, "fasta": { "type": "string", "fa_icon": "fas fa-font", "description": "Path to FASTA genome file.", "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." }, + "mature": { + "type": "string", + "description": "Path to FASTA file with mature miRNAs.", + "fa_icon": "fas fa-wheelchair" + }, + "hairpin": { + "type": "string", + "description": "Path to FASTA file with miRNAs precursors.", + "fa_icon": "fab fa-cuttlefish" + }, + "bt_index": { + "type": "string", + "description": "Path to the Bowtie 1 reference genome index.", + "fa_icon": "fas fa-indent" + }, + "references_parsed": { + "type": "string", + "description": "Path to the parsed reference folder containing the files for the miRDeep2.", + "fa_icon": "fas fa-align-left" + }, + "saveReference": { + "type": "boolean", + "default": true, + "description": "Save generated reference genome files to results.", + "help_text": "Saving generated references means that you can use them for future pipeline runs, reducing processing times.", + "fa_icon": "fas fa-save" + }, "igenomes_base": { "type": "string", "description": "Directory / URL base for iGenomes references.", @@ -69,6 +115,66 @@ } } }, + "trimming_options": { + "title": "Trimming options", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-cut", + "properties": { + "min_length": { + "type": "integer", + "default": 17, + "description": "Discard reads that are shorter than this after quality / adapter trimming.", + "fa_icon": "fas fa-ruler" + }, + "clip_R1": { + "type": "integer", + "fa_icon": "fas fa-cut", + "description": "Bp to remove from the 5' end of read 1" + }, + "three_prime_clip_R1": { + "type": "integer", + "fa_icon": "fas fa-cut", + "description": "Bp to remove from the 3' end of read 1 AFTER adapter/quality trimming has been performed" + }, + "three_prime_adapter": { + "type": "string", + "default": "TGGAATTCTCGGGTGCCAAGG", + "fa_icon": "fas fa-text-width", + "description": "Sequencing adapter sequence to search for and remove." + } + } + }, + "skipping_pipeline_steps": { + "title": "Skipping pipeline steps", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-fast-forward", + "properties": { + "skipQC": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip all QC steps" + }, + "skipFastqc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip FastQC" + }, + "skip_mirdeep": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip miRDeep" + }, + "skipMultiqc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip MultiQC" + } + } + }, "generic_options": { "title": "Generic options", "type": "object", @@ -105,6 +211,11 @@ "hidden": true, "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." }, + "seq_center": { + "type": "string", + "description": "Text about sequencing center which will be added in the header of output bam files.", + "fa_icon": "fas fa-fingerprint" + }, "email_on_fail": { "type": "string", "description": "Email address for completion summary, only when pipeline fails.", @@ -240,6 +351,12 @@ { "$ref": "#/definitions/reference_genome_options" }, + { + "$ref": "#/definitions/trimming_options" + }, + { + "$ref": "#/definitions/skipping_pipeline_steps" + }, { "$ref": "#/definitions/generic_options" }, @@ -249,61 +366,5 @@ { "$ref": "#/definitions/institutional_config_options" } - ], - "properties": { - "protocol": { - "type": "string", - "default": "illumina" - }, - "clip_R1": { - "type": "integer", - "default": 0 - }, - "three_prime_clip_R1": { - "type": "integer", - "default": 0 - }, - "three_prime_adapter": { - "type": "string", - "default": "TGGAATTCTCGGGTGCCAAGG" - }, - "min_length": { - "type": "integer", - "default": 17 - }, - "skipQC": { - "type": "string" - }, - "skipFastqc": { - "type": "string" - }, - "skipMultiqc": { - "type": "string" - }, - "skip_mirdeep": { - "type": "string" - }, - "saveReference": { - "type": "string", - "default": "true" - }, - "seq_center": { - "type": "string" - }, - "references_parsed": { - "type": "string" - }, - "bt_index": { - "type": "string" - }, - "mature": { - "type": "string" - }, - "hairpin": { - "type": "string" - }, - "mirtrace_species": { - "type": "string" - } - } + ] } \ No newline at end of file From 8e4458190b9df61f15c4be3de2f778843ed45eb7 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Sat, 7 Nov 2020 00:54:31 +0100 Subject: [PATCH 065/146] Updated CamelCase and Upper Case parameters to be lower_case_snake_make --- CHANGELOG.md | 39 +++++++++++++++++++-------------- environment.yml | 4 ++-- main.nf | 52 ++++++++++++++++++++++---------------------- nextflow.config | 12 +++++----- nextflow_schema.json | 16 +++++++------- 5 files changed, 65 insertions(+), 58 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d0788018..09a3c038 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,27 +6,34 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v1.1dev - [date] * Change `--reads` to `--input` for consistency with rest of nf-core +* Made `CamelCase` pipeline parameters `snake_case` and lower case + * `clip_R1` -> `clip_r1` + * `three_prime_clip_R1` -> `three_prime_clip_r1` + * `saveReference` -> `save_reference` + * `skipQC` -> `skip_qc` + * `skipFastqc` -> `skip_fastqc` + * `skipMultiqc` -> `skip_multiqc` * Move all parameter documentation into new `nextflow_schema.json` file. -* Update with the latest TEMPLATE version and nf-core 1.11 +* Update with the latest `TEMPLATE` version for nf-core `v1.11` * Update conda environment with new packages and updates ### Packaged software updates -* fastqc=0.11.8 -> 0.11.9 -* trim-galore=0.6.3 -> 0.6.5 -* bowtie=1.2.2 -> 1.2.3 -* multiqc=1.7 -> 1.8 -* mirtop=0.4.22 -> 0.4.23 -* seqcluster=1.2.5 -> 1.2.7 -* htseq=0.11.2 -> 0.11.3 -* fastx_toolkit=0.0.14 -> 0.0.14 -* seqkit=0.10.1 -> 0.12.0 -* mirtrace=1.0.0 -> 1.0.1 -* Added conda-forge::python=3.7.3 -* Added conda-forge::markdown=3.1.1 -* Added conda-forge::pymdown-extensions=6.0 -* Added conda-forge::pygments=2.5.2 -* Removed conda-forge::r-markdown=1.0 +* `fastqc=0.11.8` -> `0.11.9` +* `trim-galore=0.6.3` -> `0.6.5` +* `bowtie=1.2.2` -> `1.2.3` +* `multiqc=1.7` -> `1.9` +* `mirtop=0.4.22` -> `0.4.23` +* `seqcluster=1.2.5` -> `1.2.7` +* `htseq=0.11.2` -> `0.11.3` +* `fastx_toolkit=0.0.14` -> `0.0.14` +* `seqkit=0.10.1` -> `0.12.0` +* `mirtrace=1.0.0` -> `1.0.1` +* Added `conda-forge::python=3.7.3` +* Added `conda-forge::markdown=3.1.1` +* Added `conda-forge::pymdown-extensions=6.0` +* Added `conda-forge::pygments=2.5.2` +* Removed `conda-forge::r-markdown=1.0` ## v1.0.0 - 2019-09-19 diff --git a/environment.yml b/environment.yml index 4a09097d..dfb129a5 100644 --- a/environment.yml +++ b/environment.yml @@ -6,7 +6,7 @@ channels: - bioconda - defaults dependencies: - ## conda-forge packages + ## conda-forge packages - conda-forge::r-base=3.6.3 - conda-forge::openjdk=11.0.1 # Needed for FastQC - conda build hangs without this - conda-forge::r-statmod=1.4.32 @@ -24,7 +24,7 @@ dependencies: - bioconda::trim-galore=0.6.5 - bioconda::samtools=1.9 - bioconda::bowtie=1.2.3 - - bioconda::multiqc=1.8 + - bioconda::multiqc=1.9 - bioconda::mirtop=0.4.23 - bioconda::seqcluster=1.2.7 - bioconda::htseq=0.11.3 diff --git a/main.nf b/main.nf index 586f042a..edb33b04 100644 --- a/main.nf +++ b/main.nf @@ -30,7 +30,7 @@ def helpMessage() { References If not specified in the configuration file or you wish to overwrite any of the references --fasta [file] Path to fasta reference - --saveReference [str] Save the generated reference files the the Results directory + --save_reference [str] Save the generated reference files the the Results directory --mature [file] Path to the FASTA file of mature miRNAs --hairpin [file] Path to the FASTA file of miRNA precursors --mirna_gtf [file] GFF/GTF file with coordinates positions of precursor and miRNAs. See: ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3 @@ -41,8 +41,8 @@ def helpMessage() { Trimming options --three_prime_adapter [str] 3’ Adapter to trim. Default: None --min_length [int] Discard reads that became shorter than length [int] because of either quality or adapter trimming. Default: 18 - --clip_R1 [int] Instructs Trim Galore to remove bp from the 5' end of read 1 - --three_prime_clip_R1 [int] Instructs Trim Galore to remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed + --clip_r1 [int] Instructs Trim Galore to remove bp from the 5' end of read 1 + --three_prime_clip_r1 [int] Instructs Trim Galore to remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed Other options: --outdir [file] The output directory where the results will be saved @@ -84,32 +84,32 @@ params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtra params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false // Define regular variables so that they can be overwritten -clip_R1 = params.clip_R1 -three_prime_clip_R1 = params.three_prime_clip_R1 +clip_r1 = params.clip_r1 +three_prime_clip_r1 = params.three_prime_clip_r1 three_prime_adapter = params.three_prime_adapter protocol = params.protocol // Presets if (params.protocol == "illumina"){ - clip_R1 = 0 - three_prime_clip_R1 = 0 + clip_r1 = 0 + three_prime_clip_r1 = 0 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" } else if (params.protocol == "nextflex"){ - clip_R1 = 4 - three_prime_clip_R1 = 4 + clip_r1 = 4 + three_prime_clip_r1 = 4 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" } else if (params.protocol == "qiaseq"){ - clip_R1 = 0 - three_prime_clip_R1 = 0 + clip_r1 = 0 + three_prime_clip_r1 = 0 three_prime_adapter = "AACTGTAGGCACCATCAAT" } else if (params.protocol == "cats"){ - clip_R1 = 3 - three_prime_clip_R1 = 0 + clip_r1 = 3 + three_prime_clip_r1 = 0 // three_prime_adapter = "GATCGGAAGAGCACACGTCTG" three_prime_adapter = "AAAAAAAA" } else { //custom protocol - clip_R1 = params.clip_R1 - three_prime_clip_R1 = params.three_prime_clip_R1 + clip_r1 = params.clip_r1 + three_prime_clip_r1 = params.three_prime_clip_r1 three_prime_adapter = params.three_prime_adapter protocol = params.protocol } @@ -211,13 +211,13 @@ summary['Run Name'] = custom_runName ?: workflow.runName summary['Input'] = params.input summary['Genome'] = params.genome summary['Min Trimmed Length'] = params.min_length -summary["Trim 5' R1"] = clip_R1 -summary["Trim 3' R1"] = three_prime_clip_R1 +summary["Trim 5' R1"] = clip_r1 +summary["Trim 3' R1"] = three_prime_clip_r1 summary['miRBase mature'] = params.mature summary['miRBase hairpin'] = params.hairpin summary['Reference Genome'] = params.fasta if(params.bt_index) summary['Bowtie Index for Ref'] = params.bt_index -summary['Save Reference'] = params.saveReference ? 'Yes' : 'No' +summary['Save Reference'] = params.save_reference ? 'Yes' : 'No' summary['Protocol'] = params.protocol summary['miRTrace species'] = params.mirtrace_species summary["3' adapter"] = three_prime_adapter @@ -311,8 +311,8 @@ if (!params.references_parsed && !params.skip_mirdeep){ */ process bowtie_indices { label 'process_medium' - publishDir path: { params.saveReference ? "${params.outdir}/references_parsed" : params.outdir }, - saveAs: { params.saveReference ? it : null }, mode: 'copy' + publishDir path: { params.save_reference ? "${params.outdir}/references_parsed" : params.outdir }, + saveAs: { params.save_reference ? it : null }, mode: 'copy' input: file refgenome from reference_genome @@ -339,8 +339,8 @@ if (!params.references_parsed && !params.skip_mirdeep){ */ process make_bowtie_index { label 'process_medium' - publishDir path: { params.saveReference ? "${params.outdir}/bowtie/reference" : params.outdir }, - saveAs: { params.saveReference ? it : null }, mode: 'copy' + publishDir path: { params.save_reference ? "${params.outdir}/bowtie/reference" : params.outdir }, + saveAs: { params.save_reference ? it : null }, mode: 'copy' input: file mature from mature @@ -378,7 +378,7 @@ process fastqc { filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" } when: - !params.skipQC && !params.skipFastqc + !params.skip_qc && !params.skip_fastqc input: file reads from raw_reads_fastqc @@ -411,8 +411,8 @@ process trim_galore { script: tg_length = "--length ${params.min_length}" - c_r1 = clip_R1 > 0 ? "--clip_R1 ${clip_R1}" : '' - tpc_r1 = three_prime_clip_R1 > 0 ? "--three_prime_clip_R1 ${three_prime_clip_R1}" : '' + c_r1 = clip_r1 > 0 ? "--clip_r1 ${clip_r1}" : '' + tpc_r1 = three_prime_clip_r1 > 0 ? "--three_prime_clip_r1 ${three_prime_clip_r1}" : '' tpa = (protocol == "qiaseq" | protocol == "cats") ? "--adapter ${three_prime_adapter}" : '--small_rna' """ trim_galore --adapter ${three_prime_adapter} $tg_length $c_r1 $tpc_r1 --max_length 40 --gzip $reads --fastqc @@ -892,7 +892,7 @@ process multiqc { publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode when: - !params.skipQC && !params.skipMultiqc + !params.skip_qc && !params.skip_multiqc input: file (multiqc_config) from ch_multiqc_config diff --git a/nextflow.config b/nextflow.config index 5248d5ba..277c7b05 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,15 +13,15 @@ params { outdir = './results' protocol = 'illumina' genome = false - clip_R1 = 0 - three_prime_clip_R1 = 0 + clip_r1 = 0 + three_prime_clip_r1 = 0 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" min_length = 17 - skipQC = false - skipFastqc = false - skipMultiqc = false + skip_qc = false + skip_fastqc = false + skip_multiqc = false skip_mirdeep = false - saveReference = true + save_reference = true seq_center = "" references_parsed = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 86c2637e..bca3751e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -25,7 +25,7 @@ "default": "illumina", "fa_icon": "fas fa-vial", "description": "Protocol for constructing smRNA-seq libraries.", - "help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_R1 = 0` `three_prime_clip_R1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_R1 = 4` `three_prime_clip_R1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_R1 = 0` `three_prime_clip_R1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_R1 = 3` `three_prime_clip_R1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |", + "help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_r1 = 4` `three_prime_clip_r1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_r1 = 3` `three_prime_clip_r1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |", "enum": [ "illumina", "nextflex", @@ -92,7 +92,7 @@ "description": "Path to the parsed reference folder containing the files for the miRDeep2.", "fa_icon": "fas fa-align-left" }, - "saveReference": { + "save_reference": { "type": "boolean", "default": true, "description": "Save generated reference genome files to results.", @@ -128,12 +128,12 @@ "description": "Discard reads that are shorter than this after quality / adapter trimming.", "fa_icon": "fas fa-ruler" }, - "clip_R1": { + "clip_r1": { "type": "integer", "fa_icon": "fas fa-cut", "description": "Bp to remove from the 5' end of read 1" }, - "three_prime_clip_R1": { + "three_prime_clip_r1": { "type": "integer", "fa_icon": "fas fa-cut", "description": "Bp to remove from the 3' end of read 1 AFTER adapter/quality trimming has been performed" @@ -153,12 +153,12 @@ "default": "", "fa_icon": "fas fa-fast-forward", "properties": { - "skipQC": { + "skip_qc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", "description": "Skip all QC steps" }, - "skipFastqc": { + "skip_fastqc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", "description": "Skip FastQC" @@ -168,7 +168,7 @@ "fa_icon": "fas fa-fast-forward", "description": "Skip miRDeep" }, - "skipMultiqc": { + "skip_multiqc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", "description": "Skip MultiQC" @@ -367,4 +367,4 @@ "$ref": "#/definitions/institutional_config_options" } ] -} \ No newline at end of file +} From 79d8134ed83ae5db1fc141646f5b83621e0021a4 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Sat, 7 Nov 2020 07:48:11 +0100 Subject: [PATCH 066/146] Tweaks to the conda environment --- environment.yml | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/environment.yml b/environment.yml index dfb129a5..8465d6ba 100644 --- a/environment.yml +++ b/environment.yml @@ -8,16 +8,15 @@ channels: dependencies: ## conda-forge packages - conda-forge::r-base=3.6.3 - - conda-forge::openjdk=11.0.1 # Needed for FastQC - conda build hangs without this - conda-forge::r-statmod=1.4.32 - conda-forge::r-data.table=1.12.4 - conda-forge::r-gplots=3.0.1.1 - conda-forge::r-r.methodss3=1.7.1 - - conda-forge::matplotlib=3.0.3 # To avoid conflics with multiple packages during docker build - conda-forge::python=3.7.3 - conda-forge::markdown=3.1.1 - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.5.2 + - conda-forge::pygments=2.6.1 + - conda-forge::pigz=2.3.4 # bioconda packages - bioconda::fastqc=0.11.9 @@ -33,4 +32,4 @@ dependencies: - bioconda::mirtrace=1.0.1 - bioconda::bioconductor-edger=3.26.5 - bioconda::bioconductor-limma=3.40.2 - - mirdeep2=2.0.1.2 + - bioconda::mirdeep2=2.0.1.2 From 6190c23ef326c9bc449d5eba38846eb11f835fd0 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Sat, 7 Nov 2020 08:06:06 +0100 Subject: [PATCH 067/146] Schema docs and changelog for --protocol custom See also #41 and #42 --- CHANGELOG.md | 1 + nextflow_schema.json | 4 ++-- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 09a3c038..aefcf6e4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 * Move all parameter documentation into new `nextflow_schema.json` file. * Update with the latest `TEMPLATE` version for nf-core `v1.11` * Update conda environment with new packages and updates +* Added `--protocol custom` to allow custom adapter trimming modes [[#41]](https://github.com/nf-core/smrnaseq/issues/41)] ### Packaged software updates diff --git a/nextflow_schema.json b/nextflow_schema.json index bca3751e..6d8825f5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -25,7 +25,7 @@ "default": "illumina", "fa_icon": "fas fa-vial", "description": "Protocol for constructing smRNA-seq libraries.", - "help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_r1 = 4` `three_prime_clip_r1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_r1 = 3` `three_prime_clip_r1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |", + "help_text": "Presets for trimming parameters and 3' adapter sequence with a specified protocol.\n\n| Protocol | Library Prep Kit | Trimming Parameter | 3' Adapter Sequence |\n| :------------ | :-------------------------------------- | :-------------------------------------- | :--------------------- |\n| illumina | Illumina TruSeq Small RNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `TGGAATTCTCGGGTGCCAAGG` |\n| nextflex | BIOO SCIENTIFIC NEXTFLEX Small RNA-Seq | `clip_r1 = 4` `three_prime_clip_r1 = 4` | `TGGAATTCTCGGGTGCCAAGG` |\n| qiaseq | QIAGEN QIAseq miRNA | `clip_r1 = 0` `three_prime_clip_r1 = 0` | `AACTGTAGGCACCATCAAT` |\n| cats | Diagenode CATS Small RNA-seq | `clip_r1 = 3` `three_prime_clip_r1 = 0` | `AAAAAAAAAAA` + `GATCGGAAGAGCACACGTCTG` (only polyA is used for trimming) |\n| custom | user defined | user defined | user defined |\n\n> NB: When running `--protocol custom` the user ***must define the 3' Adapter Sequence***.\n> If trimming parameters aren't provided the pipeline will deafult to `clip_R1 = 0` and `three_prime_clip_R1 = 0` (i.e. no extra clipping).", "enum": [ "illumina", "nextflex", @@ -367,4 +367,4 @@ "$ref": "#/definitions/institutional_config_options" } ] -} +} \ No newline at end of file From 018d9da638458b6af43ed108a2049db15cc54f13 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 12 Nov 2020 01:30:46 +0100 Subject: [PATCH 068/146] Add s3 data paths to test_full.config --- CHANGELOG.md | 1 + conf/test.config | 2 +- conf/test_full.config | 37 +++++++++++++++++++++++++++++++++---- 3 files changed, 35 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 15b83a6a..b93c01c0 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,7 @@ * Update conda environment with new packages and updates * Added `--protocol custom` to allow custom adapter trimming modes [[#41]](https://github.com/nf-core/smrnaseq/issues/41)] * Fix error when UMI is on the reads header: [[#35](https://github.com/nf-core/smrnaseq/issues/35)] +* Add full-size benchmark / test dataset to run on AWS for each release (see `test_full.config`) ### Packaged software updates diff --git a/conf/test.config b/conf/test.config index aa5edfd3..3ae89828 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,7 +29,7 @@ params { 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.4.ebwt', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt', 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genome.rev.1.ebwt' -] + ] gtf = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/genes.gtf' mature = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/mature.fa' hairpin = 'https://github.com/nf-core/test-datasets/raw/smrnaseq/reference/hairpin.fa' diff --git a/conf/test_full.config b/conf/test_full.config index 8461afe9..477c3c8f 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -12,10 +12,39 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398625_GSM2560978_control_preclinic3_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398623_GSM2560976_control_preclinic1_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398626_GSM2560979_control_preclinic4_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398624_GSM2560977_control_preclinic2_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398627_GSM2560980_control_preclinic5_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398628_GSM2560981_control_preclinic6_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398631_GSM2560984_preclinic3_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398629_GSM2560982_preclinic1_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398630_GSM2560983_preclinic2_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398632_GSM2560985_preclinic4_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398633_GSM2560986_preclinic5_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398634_GSM2560987_preclinic6_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398636_GSM2560989_preclinic7_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398639_GSM2560992_control_clinic3_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398635_GSM2560988_control_preclinic7_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398638_GSM2560991_control_clinic2_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398637_GSM2560990_control_clinic1_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398640_GSM2560993_control_clinic4_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398641_GSM2560994_control_clinic5_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398643_GSM2560996_clinic1_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398642_GSM2560995_control_clinic6_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398644_GSM2560997_clinic2_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398645_GSM2560998_clinic3_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398646_GSM2560999_clinic4_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398649_GSM2561002_control_clinic7_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398647_GSM2561000_clinic5_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398648_GSM2561001_clinic6_Homo_sapiens_ncRNA-Seq.fastq.gz', + 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398650_GSM2561003_clinic7_Homo_sapiens_ncRNA-Seq.fastq.gz' ] + + genome = 'GRCh37' + mirtrace_species = "hsa" } + + From c11602ab0e4cfe7d69130bf87b2953f44930241b Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 12 Nov 2020 13:16:30 +0100 Subject: [PATCH 069/146] Add new profile for test_full --- nextflow.config | 1 + 1 file changed, 1 insertion(+) diff --git a/nextflow.config b/nextflow.config index 277c7b05..f365de08 100644 --- a/nextflow.config +++ b/nextflow.config @@ -85,6 +85,7 @@ profiles { podman.enabled = true } test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } } // Load igenomes.config if required From a29cc7f3f8845d0f4c388aab84f84a107c1c2819 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 12 Nov 2020 13:30:05 +0100 Subject: [PATCH 070/146] Found several params missing from nextflow.config --- nextflow.config | 11 ++++++++++- nextflow_schema.json | 6 ++++++ 2 files changed, 16 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index f365de08..3952ff78 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,7 +12,17 @@ params { input = "data/*.fastq.gz" outdir = './results' protocol = 'illumina' + + // Reference genomes genome = false + mature = false + hairpin = false + mirna_gtf = false + fasta = false + bt_index = false + mirtrace_species = false + references_parsed = false + clip_r1 = 0 three_prime_clip_r1 = 0 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" @@ -23,7 +33,6 @@ params { skip_mirdeep = false save_reference = true seq_center = "" - references_parsed = false // Boilerplate options name = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 6d8825f5..e4a8655c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -72,6 +72,12 @@ "description": "Path to FASTA genome file.", "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." }, + "mirna_gtf": { + "type": "string", + "description": "GFF/GTF file with coordinates positions of precursor and miRNAs.", + "help_text": "This can be downloaded from [`ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3`](ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3)", + "fa_icon": "fas fa-address-book" + }, "mature": { "type": "string", "description": "Path to FASTA file with mature miRNAs.", From d4966887f0deee25aee640dacbc2167f5a7104dd Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:26:05 +0100 Subject: [PATCH 071/146] Added params.mirtrace_species to all genomes in igenomes.config --- conf/igenomes.config | 37 +++++++++++++++++++++++++++++++++++++ main.nf | 4 ++-- 2 files changed, 39 insertions(+), 2 deletions(-) diff --git a/conf/igenomes.config b/conf/igenomes.config index caeafceb..088a3a60 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -22,6 +22,7 @@ params { mito_name = "MT" macs_gsize = "2.7e9" blacklist = "${baseDir}/assets/blacklists/GRCh37-blacklist.bed" + mirtrace_species = "hsa" } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" @@ -34,6 +35,7 @@ params { mito_name = "chrM" macs_gsize = "2.7e9" blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + mirtrace_species = "hsa" } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" @@ -47,6 +49,7 @@ params { mito_name = "MT" macs_gsize = "1.87e9" blacklist = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed" + mirtrace_species = "mmu" } 'TAIR10' { fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" @@ -58,6 +61,7 @@ params { bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" mito_name = "Mt" + mirtrace_species = "ath" } 'EB2' { fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" @@ -68,6 +72,7 @@ params { gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + // mirtrace_species = "bsu" } 'UMD3.1' { fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" @@ -79,6 +84,7 @@ params { bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" mito_name = "MT" + mirtrace_species = "bta" } 'WBcel235' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" @@ -90,6 +96,7 @@ params { bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" mito_name = "MtDNA" macs_gsize = "9e7" + mirtrace_species = "cel" } 'CanFam3.1' { fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" @@ -101,6 +108,7 @@ params { bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" mito_name = "MT" + mirtrace_species = "cfa" } 'GRCz10' { fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" @@ -111,6 +119,7 @@ params { gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" mito_name = "MT" + mirtrace_species = "dre" } 'BDGP6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" @@ -122,6 +131,7 @@ params { bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" mito_name = "M" macs_gsize = "1.2e8" + mirtrace_species = "dme" } 'EquCab2' { fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" @@ -133,6 +143,7 @@ params { bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" mito_name = "MT" + // mirtrace_species = "ecb" } 'EB1' { fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" @@ -143,6 +154,7 @@ params { gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + // mirtrace_species = "ecd" } 'Galgal4' { fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" @@ -153,6 +165,7 @@ params { gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" mito_name = "MT" + mirtrace_species = "gga" } 'Gm01' { fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" @@ -163,6 +176,7 @@ params { gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + // mirtrace_species = "gmx" } 'Mmul_1' { fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" @@ -174,6 +188,7 @@ params { bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" mito_name = "MT" + // mirtrace_species = "mcc" } 'IRGSP-1.0' { fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" @@ -184,6 +199,7 @@ params { gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" mito_name = "Mt" + mirtrace_species = "osa" } 'CHIMP2.1.4' { fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" @@ -195,6 +211,7 @@ params { bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" mito_name = "MT" + mirtrace_species = "ptr" } 'Rnor_6.0' { fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" @@ -205,6 +222,7 @@ params { gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" mito_name = "MT" + mirtrace_species = "rno" } 'R64-1-1' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" @@ -216,6 +234,7 @@ params { bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" mito_name = "MT" macs_gsize = "1.2e7" + // mirtrace_species = "sce" } 'EF2' { fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" @@ -228,6 +247,7 @@ params { readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" mito_name = "MT" macs_gsize = "1.21e7" + // mirtrace_species = "spo" } 'Sbi1' { fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" @@ -238,6 +258,7 @@ params { gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + mirtrace_species = "sbi" } 'Sscrofa10.2' { fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" @@ -249,6 +270,7 @@ params { bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" mito_name = "MT" + mirtrace_species = "ssc" } 'AGPv3' { fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" @@ -259,6 +281,7 @@ params { gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" mito_name = "Mt" + mirtrace_species = "zma" } 'hg38' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" @@ -271,6 +294,7 @@ params { mito_name = "chrM" macs_gsize = "2.7e9" blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + mirtrace_species = "hsa" } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" @@ -284,6 +308,7 @@ params { mito_name = "chrM" macs_gsize = "2.7e9" blacklist = "${baseDir}/assets/blacklists/hg19-blacklist.bed" + mirtrace_species = "hsa" } 'mm10' { fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" @@ -297,6 +322,7 @@ params { mito_name = "chrM" macs_gsize = "1.87e9" blacklist = "${baseDir}/assets/blacklists/mm10-blacklist.bed" + mirtrace_species = "mmu" } 'bosTau8' { fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" @@ -307,6 +333,7 @@ params { gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" mito_name = "chrM" + mirtrace_species = "bta" } 'ce10' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" @@ -319,6 +346,7 @@ params { readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" mito_name = "chrM" macs_gsize = "9e7" + mirtrace_species = "cel" } 'canFam3' { fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" @@ -330,6 +358,7 @@ params { bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" mito_name = "chrM" + mirtrace_species = "cfa" } 'danRer10' { fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" @@ -341,6 +370,7 @@ params { bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "1.37e9" + mirtrace_species = "dre" } 'dm6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" @@ -352,6 +382,7 @@ params { bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "1.2e8" + mirtrace_species = "dme" } 'equCab2' { fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" @@ -363,6 +394,7 @@ params { bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" mito_name = "chrM" + // mirtrace_species = "ecb" } 'galGal4' { fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" @@ -374,6 +406,7 @@ params { bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" mito_name = "chrM" + mirtrace_species = "gga" } 'panTro4' { fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" @@ -385,6 +418,7 @@ params { bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" mito_name = "chrM" + mirtrace_species = "ptr" } 'rn6' { fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" @@ -395,6 +429,7 @@ params { gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" mito_name = "chrM" + mirtrace_species = "rno" } 'sacCer3' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" @@ -405,6 +440,7 @@ params { readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" mito_name = "chrM" macs_gsize = "1.2e7" + // mirtrace_species = "sce" } 'susScr3' { fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" @@ -416,6 +452,7 @@ params { bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" mito_name = "chrM" + mirtrace_species = "ssc" } } } diff --git a/main.nf b/main.nf index c6af4bf4..67a1c2dd 100644 --- a/main.nf +++ b/main.nf @@ -116,9 +116,9 @@ if (params.protocol == "illumina"){ if (!params.mirna_gtf && params.mirtrace_species){ mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) -}else if (params.mirna_gtf) { +} else if (params.mirna_gtf) { mirna_gtf = file(params.mirna_gtf, checkIfExists: true) -}else{ +} else { mirna_gtf = false } From cc1d3a09753bd8cf3dc925c0915c694bd2c30798 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:31:05 +0100 Subject: [PATCH 072/146] Update GitHub Actions CI workflows See https://github.com/nf-core/tools/pull/774 --- .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/ci.yml | 4 ++-- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 84250fbe..82521b67 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -15,7 +15,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Setup Miniconda - uses: goanpeca/setup-miniconda@v1.0.2 + uses: conda-incubator/setup-miniconda@v2 with: auto-update-conda: true python-version: 3.7 diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 5e62f62f..eeab8b33 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Setup Miniconda - uses: goanpeca/setup-miniconda@v1.0.2 + uses: conda-incubator/setup-miniconda@v2 with: auto-update-conda: true python-version: 3.7 diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 464b7f7a..f44099bc 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -26,9 +26,9 @@ jobs: uses: actions/checkout@v2 - name: Check if Dockerfile or Conda environment changed - uses: technote-space/get-diff-action@v1 + uses: technote-space/get-diff-action@v4 with: - PREFIX_FILTER: | + FILES: | Dockerfile environment.yml From e189514263bb045c26edccaffa5bba12984b5222 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:45:28 +0100 Subject: [PATCH 073/146] miRBase mature / hairpin - default to miRBase FTP URLs Add to workflow to gunzip these files if they end with .gz --- conf/test_full.config | 3 +-- main.nf | 17 +++++++++++++++-- nextflow.config | 2 ++ 3 files changed, 18 insertions(+), 4 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 477c3c8f..7a26ee7f 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -43,8 +43,7 @@ params { 's3://nf-core-awsmegatests/smrnaseq/input_data/SRR5398650_GSM2561003_clinic7_Homo_sapiens_ncRNA-Seq.fastq.gz' ] - genome = 'GRCh37' - mirtrace_species = "hsa" + genome = 'GRCh38' } diff --git a/main.nf b/main.nf index 67a1c2dd..3f6d620a 100644 --- a/main.nf +++ b/main.nf @@ -78,8 +78,6 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome // Genome options params.bt_index = params.genome ? params.genomes[ params.genome ].bowtie ?: false : false -params.mature = params.genome ? params.genomes[ params.genome ].mature ?: false : false -params.hairpin = params.genome ? params.genomes[ params.genome ].hairpin ?: false : false params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtrace_species ?: false : false params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false @@ -327,9 +325,24 @@ if (!params.references_parsed && !params.skip_mirdeep){ script: """ + # Uncompress FASTA reference files if necessary + MATURE="$mature" + HAIRPIN="$hairpin" + if [ \${MATURE: -3} == ".gz" ]; then + gunzip "$mature" + fi + if [ \${HAIRPIN: -3} == ".gz" ]; then + gunzip "$hairpin" + fi + + # Remove any special base characters from reference genome FASTA file sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome + + # Remove spaces from miRBase FASTA files sed -i 's, ,_,g' $hairpin sed -i 's, ,_,g' $mature + + # Build bowtie index bowtie-build $refgenome genome """ } diff --git a/nextflow.config b/nextflow.config index 3952ff78..a764d210 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,6 +22,8 @@ params { bt_index = false mirtrace_species = false references_parsed = false + mature = "ftp://mirbase.org/pub/mirbase/CURRENT/mature.fa.gz" + hairpin = "ftp://mirbase.org/pub/mirbase/CURRENT/hairpin.fa.gz" clip_r1 = 0 three_prime_clip_r1 = 0 From cf0903e077a331786663fd25850196d32e08a05d Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:49:26 +0100 Subject: [PATCH 074/146] Update pipeline schema help for params.mature and params.hairpin --- nextflow_schema.json | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index e4a8655c..9972d7c8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -81,12 +81,16 @@ "mature": { "type": "string", "description": "Path to FASTA file with mature miRNAs.", - "fa_icon": "fas fa-wheelchair" + "fa_icon": "fas fa-wheelchair", + "help_text": "Typically this will be the `mature.fa` file from miRBase. Can be given either as a plain text `.fa` file or a compressed `.gz` file.\n\nDefaults to the current miRBase release URL, from which the file will be downloaded.", + "default": "ftp://mirbase.org/pub/mirbase/CURRENT/mature.fa.gz" }, "hairpin": { "type": "string", "description": "Path to FASTA file with miRNAs precursors.", - "fa_icon": "fab fa-cuttlefish" + "fa_icon": "fab fa-cuttlefish", + "help_text": "Typically this will be the `mature.fa` file from miRBase. Can be given either as a plain text `.fa` file or a compressed `.gz` file.\n\nDefaults to the current miRBase release URL, from which the file will be downloaded.", + "default": "ftp://mirbase.org/pub/mirbase/CURRENT/hairpin.fa.gz" }, "bt_index": { "type": "string", From cdf3669767efa6c8d3290b97665de219c1c38e79 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:53:35 +0100 Subject: [PATCH 075/146] Refactor init code for params.mirna_gtf --- main.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index 3f6d620a..50c087fd 100644 --- a/main.nf +++ b/main.nf @@ -80,6 +80,7 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome params.bt_index = params.genome ? params.genomes[ params.genome ].bowtie ?: false : false params.mirtrace_species = params.genome ? params.genomes[ params.genome ].mirtrace_species ?: false : false params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false +params.mirna_gtf = params.mirtrace_species ? "ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3" : false // Define regular variables so that they can be overwritten clip_r1 = params.clip_r1 @@ -112,9 +113,7 @@ if (params.protocol == "illumina"){ protocol = params.protocol } -if (!params.mirna_gtf && params.mirtrace_species){ - mirna_gtf = file("ftp://mirbase.org/pub/mirbase/CURRENT/genomes/${params.mirtrace_species}.gff3", checkIfExists: true) -} else if (params.mirna_gtf) { +if (params.mirna_gtf) { mirna_gtf = file(params.mirna_gtf, checkIfExists: true) } else { mirna_gtf = false From bf8b8534041a9b6ab2921582ceb4d8165f478ea4 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:56:16 +0100 Subject: [PATCH 076/146] Changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index b93c01c0..7a343fe8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,9 @@ * Update conda environment with new packages and updates * Added `--protocol custom` to allow custom adapter trimming modes [[#41]](https://github.com/nf-core/smrnaseq/issues/41)] * Fix error when UMI is on the reads header: [[#35](https://github.com/nf-core/smrnaseq/issues/35)] +* Updated `params.mirtrace_species` to be properly initialised when using `--genome`, for all iGenomes species +* Made `params.mature` and `params.hairpin` default to miRBase FTP URL so that the file is automatically downloaded if not provided +* Allow `.fa` or `.fa.gz` files for mature and hairpin FASTA files. * Add full-size benchmark / test dataset to run on AWS for each release (see `test_full.config`) ### Packaged software updates From 28e59cfe43c0186ef4605f32a1c35c68d9ba81d6 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 22:59:10 +0100 Subject: [PATCH 077/146] Improve docs for params.mirna_gtf --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 9972d7c8..eede33e7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -69,13 +69,13 @@ "fasta": { "type": "string", "fa_icon": "fas fa-font", - "description": "Path to FASTA genome file.", + "description": "Path to reference genome FASTA genome file.", "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." }, "mirna_gtf": { "type": "string", "description": "GFF/GTF file with coordinates positions of precursor and miRNAs.", - "help_text": "This can be downloaded from [`ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3`](ftp://mirbase.org/pub/mirbase/CURRENT/genomes/hsa.gff3)", + "help_text": "miRBase `.gff3` file, typically downloadedfrom [`ftp://mirbase.org/pub/mirbase/CURRENT/genomes/`](ftp://mirbase.org/pub/mirbase/CURRENT/genomes/)\n\nIf using iGenomes with `--genome` this file will be downloaded from miRBase automatically during the pipeline run.\n\n", "fa_icon": "fas fa-address-book" }, "mature": { From 90f1a4840b2fed3b77eff82698d28eb1da2cb2cb Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 16 Nov 2020 23:05:24 +0100 Subject: [PATCH 078/146] Code cleanup --- main.nf | 51 ++++++++++++++++++++------------------------------- 1 file changed, 20 insertions(+), 31 deletions(-) diff --git a/main.nf b/main.nf index 50c087fd..baf6675a 100644 --- a/main.nf +++ b/main.nf @@ -120,41 +120,30 @@ if (params.mirna_gtf) { } // Validate inputs - if (params.skip_mirdeep){ - if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } - if (params.gtf) { gtf = file(params.gtf, checkIfExists: true) } - indices_mirdeep2 = Channel.empty() - fasta = Channel.empty() -} -else{ - if (params.references_parsed){Channel.empty() - fasta = file("$params.references_parsed/genome.fa", checkIfExists: true) - hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) - mature = file("$params.references_parsed/mature.fa", checkIfExists: true) - indices_mirdeep2 = Channel - .fromPath("$params.references_parsed/genome.*.ebwt", checkIfExists: true) - .ifEmpty { exit 1, "Reference parsed genome indices not found: ${references_parsed}"} - } - else{ - if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } - if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } - if (params.fasta) {reference_genome = file(params.fasta, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.fasta}" } - } + if (params.mature) { mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } + if (params.hairpin) { hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } + indices_mirdeep2 = Channel.empty() + fasta = Channel.empty() +} else { + if (params.references_parsed){ + fasta = file("$params.references_parsed/genome.fa", checkIfExists: true) + hairpin = file("$params.references_parsed/hairpin.fa", checkIfExists: true) + mature = file("$params.references_parsed/mature.fa", checkIfExists: true) + indices_mirdeep2 = Channel.fromPath("$params.references_parsed/genome.*.ebwt", checkIfExists: true).ifEmpty { exit 1, "Reference parsed genome indices not found: ${references_parsed}"} + } else { + if (params.mature) { reference_mature = file(params.mature, checkIfExists: true) } else { exit 1, "Mature file not found: ${params.mature}" } + if (params.hairpin) { reference_hairpin = file(params.hairpin, checkIfExists: true) } else { exit 1, "Hairpin file not found: ${params.hairpin}" } + if (params.fasta) { reference_genome = file(params.fasta, checkIfExists: true) } else { exit 1, "Reference genome file not found: ${params.fasta}" } + } } if( params.bt_index ){ - bt_indices = Channel - .fromPath("${params.bt_index}*", checkIfExists: true) - .ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } -} - -else if( params.bt_indices ){ - bt_indices = Channel.from(params.input_paths).map{ file(it) }.toList() -} -if( !params.bt_index) { - log.info "No GTF / Bowtie 1 index supplied - host reference genome analysis will be skipped." + bt_indices = Channel.fromPath("${params.bt_index}*", checkIfExists: true).ifEmpty { exit 1, "Bowtie1 index directory not found: ${bt_dir}" } +} else if( params.bt_indices ){ + bt_indices = Channel.from(params.bt_indices).map{ file(it) }.toList() +} else { + log.info "No Bowtie 1 index supplied - host reference genome analysis will be skipped." } if( !params.mirtrace_species ){ exit 1, "Reference species for miRTrace is not defined." From 5c73237125c5091b215596de4ac28cf681b75eaf Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 19 Nov 2020 13:47:49 +0000 Subject: [PATCH 079/146] Template update for nf-core/tools version 1.12 --- .github/ISSUE_TEMPLATE/bug_report.md | 7 ++++- .github/ISSUE_TEMPLATE/config.yml | 8 +++++ .github/ISSUE_TEMPLATE/feature_request.md | 6 ++++ .github/workflows/awsfulltest.yml | 7 +++-- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 12 ++++---- .github/workflows/linting.yml | 13 +++++++-- .github/workflows/linting_comment.yml | 29 +++++++++++++++++++ .github/workflows/push_dockerhub_dev.yml | 28 ++++++++++++++++++ ...ckerhub.yml => push_dockerhub_release.yml} | 15 ++-------- CHANGELOG.md | 2 +- Dockerfile | 6 ++-- README.md | 2 +- assets/sendmail_template.txt | 2 +- environment.yml | 2 +- main.nf | 14 ++++----- nextflow.config | 7 +++-- 18 files changed, 121 insertions(+), 43 deletions(-) create mode 100644 .github/ISSUE_TEMPLATE/config.yml create mode 100644 .github/workflows/linting_comment.yml create mode 100644 .github/workflows/push_dockerhub_dev.yml rename .github/workflows/{push_dockerhub.yml => push_dockerhub_release.yml} (68%) diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 1c58337b..9bd74c46 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -1,10 +1,15 @@ +--- +name: Bug report +about: Report something that is broken or incorrect +labels: bug +--- + diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml new file mode 100644 index 00000000..ef66ebca --- /dev/null +++ b/.github/ISSUE_TEMPLATE/config.yml @@ -0,0 +1,8 @@ +blank_issues_enabled: false +contact_links: + - name: Join nf-core + url: https://nf-co.re/join + about: Please join the nf-core community here + - name: "Slack #smrnaseq channel" + url: https://nfcore.slack.com/channels/smrnaseq + about: Discussion about the nf-core/smrnaseq pipeline diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index 58594d49..bea7e5e9 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,3 +1,9 @@ +--- +name: Feature request +about: Suggest an idea for the nf-core website +labels: enhancement +--- + +## Check Documentation + +I have checked the following places for your error: + +- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) +- [ ] [nf-core/smrnaseq pipeline documentation](https://nf-co.re/nf-core/smrnaseq/usage) + ## Description of the bug @@ -28,6 +35,13 @@ Steps to reproduce the behaviour: +## Log files + +Have you provided the following extra information/files: + +- [ ] The command used to run the pipeline +- [ ] The `.nextflow.log` file + ## System - Hardware: diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index d178daf0..bddc471c 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -13,8 +13,14 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn ## PR checklist -- [ ] This comment contains a description of changes (with reason) -- [ ] `CHANGELOG.md` is updated +- [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] Documentation in `docs` is updated -- [ ] If necessary, also make a PR on the [nf-core/smrnaseq branch on the nf-core/test-datasets repo](https://github.com/nf-core/test-datasets/pull/new/nf-core/smrnaseq) + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. +- [ ] Make sure your code lints (`nf-core lint .`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). +- [ ] Usage Documentation in `docs/usage.md` is updated. +- [ ] Output Documentation in `docs/output.md` is updated. +- [ ] `CHANGELOG.md` is updated. +- [ ] `README.md` is updated (including new tool citations and authors/contributors). diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml index 96b12a70..8d7eb53b 100644 --- a/.github/markdownlint.yml +++ b/.github/markdownlint.yml @@ -1,5 +1,12 @@ # Markdownlint configuration file -default: true, +default: true line-length: false no-duplicate-header: siblings_only: true +no-inline-html: + allowed_elements: + - img + - p + - kbd + - details + - summary diff --git a/Dockerfile b/Dockerfile index 8c6f9883..42c3b807 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.12 +FROM nfcore/base:1.12.1 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" diff --git a/README.md b/README.md index c5baa702..141fa267 100644 --- a/README.md +++ b/README.md @@ -12,6 +12,9 @@ ## Introduction + +**nf-core/smrnaseq** is a bioinformatics best-practise analysis pipeline for + The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. ## Quick Start @@ -38,6 +41,15 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool See [usage docs](https://nf-co.re/smrnaseq/usage) for all of the available options when running the pipeline. +## Pipeline Summary + +By default, the pipeline currently performs the following: + + + +* Sequencing quality control (`FastQC`) +* Overall pipeline run summaries (`MultiQC`) + ## Documentation The nf-core/smrnaseq pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/smrnaseq/usage) and [output](https://nf-co.re/smrnaseq/output). @@ -48,13 +60,18 @@ The nf-core/smrnaseq pipeline comes with documentation about the pipeline: [usag nf-core/smrnaseq was originally written by Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano . +We thank the following people for their extensive assistance in the development +of this pipeline: + + + ## Contributions and Support If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). For further information or help, don't hesitate to get in touch on the [Slack `#smrnaseq` channel](https://nfcore.slack.com/channels/smrnaseq) (you can join with [this invite](https://nf-co.re/join/slack)). -## Citation +## Citations @@ -67,3 +84,7 @@ You can cite the `nf-core` publication as follows: > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). > ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) + +In addition, references of tools and data used in this pipeline are as follows: + + diff --git a/assets/nf-core-smrnaseq_logo.png b/assets/nf-core-smrnaseq_logo.png index 62f94cbfccb05016b4f4d7abbcf74c80eddb3e97..f7dc63c3b9894b496bd1cb9d5ec115729d8fd196 100644 GIT binary patch literal 110462 zcmeFXhgZ{C_dOiI5tXK*BGP7%BGP+J7)KNX0tzY}M4I#_EtKG(AW|X;y{a@30#ZT` zMMfZ0r6lwsozP1Hge30=N9Xzd5AXM`Wm&qox%ZrX_SyT~MBUTZW;=7`3oJc83c=9X{HRe8B&y@}tlE6%`$=cv5>836b}!x*FMFwuPp85=C9ZN__~I|+w-J&> 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"${baseDir}/assets/blacklists/GRCh37-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" @@ -33,7 +33,7 @@ params { bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" @@ -46,7 +46,7 @@ params { readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" mito_name = "MT" macs_gsize = "1.87e9" - blacklist = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" } 'TAIR10' { fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" @@ -270,7 +270,7 @@ params { bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" @@ -283,7 +283,7 @@ params { readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg19-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" } 'mm10' { fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" @@ -296,7 +296,7 @@ params { readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" mito_name = "chrM" macs_gsize = "1.87e9" - blacklist = "${baseDir}/assets/blacklists/mm10-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" } 'bosTau8' { fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" diff --git a/docs/images/nf-core-smrnaseq_logo.png b/docs/images/nf-core-smrnaseq_logo.png index f22165472c736a9a961ab861f43e0baead75d99b..f7dc63c3b9894b496bd1cb9d5ec115729d8fd196 100644 GIT binary patch literal 110462 zcmeFXhgZ{C_dOiI5tXK*BGP7%BGP+J7)KNX0tzY}M4I#_EtKG(AW|X;y{a@30#ZT` zMMfZ0r6lwsozP1Hge30=N9Xzd5AXM`Wm&qox%ZrX_SyT~MBUTZW;=7`3oJc83c=9X{HRe8B&y@}tlE6%`$=cv5>836b}!x*FMFwuPp85=C9ZN__~I|+w-J&> z^V&H7`>pfRCu&%xcTyR8N5X^psFDt(>F*j-6pAo#)c;%m7lHqa!2j{w zF>{Epu$Y>fa%Xl1E-WraCR@Rr;`qU4*1oGIS^TjpW#6t5EJFj;VM7ns9`rGJUjN^w zCWE!LwKMhzh*@d<(RLCOvwop`-xO^m8@s(dXNm~Yz~w>l4tOFnotPJ??-%zamp?6? z$7*j5r3fuK?A@>+Ts);>#Hji6kQSdVUAh#Z>TMVBj8IZlS64?H*R;+^Pd{jFSsGQk zVgcsebusV4EK@rTyQ<^bb4DhTtwJZn{VcsLm-F^^d958b#8A}3Ig~-@UST_bJzPdU 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zNQ*lL9>X@q9@xPERU^0IEyz@&xIKf|C-V8X@QMu<7r__M4sq&^~-;I zU9Syx)w@hA+vZFjdrpDVU)R_)&T*HC7jl}C~9tP4QiecMssZ|p|@ zD8>Y(CbUPcyl(&Go{pw2}<(TaF#tD@g6fo-Up@eHFf`e1Q+ddlK#*Be4 zs8{PyekG=SUVgNgpc9EjZd0}A)CIGyeP_Rn={l@8&yB4Fy0Zk}Upv!rMZ?NSsUz^V z2Q~OMulp(C2bp0eOjPbU&6&QHQ2Vt$!bxSD2}9)HazbRn{&SBJL}X^?175-q=I;NG zhJl0kfJ|7GBn~jZ9=Yil^YGRz^g7=EK_;j#skx81oEJVNYhAy5B}3?#z-zUf;{FHH CQh}`i diff --git a/main.nf b/main.nf index a273adac..89375590 100644 --- a/main.nf +++ b/main.nf @@ -336,7 +336,7 @@ workflow.onComplete { def email_html = html_template.toString() // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() From 188de308ad0c7b46e4d6dd44a6c0f3ae94fce04d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Fri, 18 Dec 2020 10:53:25 +0000 Subject: [PATCH 083/146] Use -f flag with gunzip to deal with soft-links --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index d403115f..4a671ca7 100644 --- a/main.nf +++ b/main.nf @@ -317,10 +317,10 @@ if (!params.references_parsed && !params.skip_mirdeep){ MATURE="$mature" HAIRPIN="$hairpin" if [ \${MATURE: -3} == ".gz" ]; then - gunzip "$mature" + gunzip -f "$mature" fi if [ \${HAIRPIN: -3} == ".gz" ]; then - gunzip "$hairpin" + gunzip -f "$hairpin" fi # Remove any special base characters from reference genome FASTA file From 48102c3d10cfb295fc18ce4f43824ba96d4e26b0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Fri, 18 Dec 2020 11:13:46 +0000 Subject: [PATCH 084/146] Update CHANGELOG --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 09418f2e..7752fbc5 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -19,6 +19,7 @@ * Made `params.mature` and `params.hairpin` default to miRBase FTP URL so that the file is automatically downloaded if not provided * Allow `.fa` or `.fa.gz` files for mature and hairpin FASTA files. * Add full-size benchmark / test dataset to run on AWS for each release (see `test_full.config`) +* Add `-f` flag to `gunzip` commands to deal with soft-links ### Packaged software updates From 338ee70be5b2ec248e8dac6e0cfc25203e0ebb23 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Fri, 18 Dec 2020 12:38:41 +0000 Subject: [PATCH 085/146] Rename files if unzipped --- main.nf | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/main.nf b/main.nf index 8f0dce68..b9c538ba 100644 --- a/main.nf +++ b/main.nf @@ -317,18 +317,20 @@ if (!params.references_parsed && !params.skip_mirdeep){ MATURE="$mature" HAIRPIN="$hairpin" if [ \${MATURE: -3} == ".gz" ]; then - gunzip -f "$mature" + gunzip -f \$MATURE + MATURE=\${MATURE: -3} fi if [ \${HAIRPIN: -3} == ".gz" ]; then - gunzip -f "$hairpin" + gunzip -f \$HAIRPIN + HAIRPIN=\${HAIRPIN: -3} fi # Remove any special base characters from reference genome FASTA file sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome # Remove spaces from miRBase FASTA files - sed -i 's, ,_,g' $hairpin - sed -i 's, ,_,g' $mature + sed -i 's, ,_,g' \$HAIRPIN + sed -i 's, ,_,g' \$MATURE # Build bowtie index bowtie-build $refgenome genome From ebf19151db7be502f51bad5c1030c154af897734 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Wed, 27 Jan 2021 22:12:16 +0000 Subject: [PATCH 086/146] Use custom genome and fix nt in FASTA --- bin/edgeR_miRBase.r | 16 +++++++++------- main.nf | 13 +++++++------ 2 files changed, 16 insertions(+), 13 deletions(-) diff --git a/bin/edgeR_miRBase.r b/bin/edgeR_miRBase.r index 4fa12c25..98aad8be 100755 --- a/bin/edgeR_miRBase.r +++ b/bin/edgeR_miRBase.r @@ -60,15 +60,15 @@ for (i in 1:2) { colnames(data)<-gsub(".stats","",basename(filelist[[i]])) colnames(unmapped)<-gsub(".stats","",basename(filelist[[i]])) - data<-data[rownames(data)!="*",] - unmapped<-unmapped[rownames(unmapped)=="*",] + data<-data[rownames(data)!="*",,drop=FALSE] + unmapped<-unmapped[rownames(unmapped)=="*",,drop=FALSE] # Write the summary table of unmapped reads write.table(unmapped,file=paste(header,"_unmapped_read_counts.txt",sep=""),sep='\t',quote=FALSE) # Remove genes with 0 reads in all samples row_sub = apply(data, 1, function(row) all(row ==0 )) - data<-data[!row_sub,] + data<-data[!row_sub,,drop=FALSE] # Normalization dataDGE<-DGEList(counts=data,genes=rownames(data)) @@ -80,10 +80,12 @@ for (i in 1:2) { dataNorm_df<-as.data.frame(cpm(dataNorm)) write.table(dataNorm_df,file=paste(header,"_normalized_CPM.txt",sep=""),sep='\t',quote=FALSE) - # Print heatmap based on normalized read counts - pdf(paste(header,"_CPM_heatmap.pdf",sep="")) - heatmap.2(cpm(dataNorm),col=redgreen(100),key=TRUE,scale="row",density.info="none",trace="none") - dev.off() + if (ncol(data)>1){ # with more than 1 sample + # Print heatmap based on normalized read counts + pdf(paste(header,"_CPM_heatmap.pdf",sep="")) + heatmap.2(cpm(dataNorm),col=redgreen(100),key=TRUE,scale="row",density.info="none",trace="none") + dev.off() + } # Make MDS plot (only perform with 3 or more samples) if (ncol(data)>2){ diff --git a/main.nf b/main.nf index b9c538ba..56617a2c 100644 --- a/main.nf +++ b/main.nf @@ -318,22 +318,22 @@ if (!params.references_parsed && !params.skip_mirdeep){ HAIRPIN="$hairpin" if [ \${MATURE: -3} == ".gz" ]; then gunzip -f \$MATURE - MATURE=\${MATURE: -3} + MATURE=\${MATURE%%.gz} fi if [ \${HAIRPIN: -3} == ".gz" ]; then gunzip -f \$HAIRPIN - HAIRPIN=\${HAIRPIN: -3} + HAIRPIN=\${HAIRPIN%%.gz} fi # Remove any special base characters from reference genome FASTA file - sed -i '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome + sed '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome > genome.fa # Remove spaces from miRBase FASTA files sed -i 's, ,_,g' \$HAIRPIN sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build $refgenome genome + bowtie-build genome.fa genome """ } } @@ -839,15 +839,16 @@ process mirdeep2 { script: - """ + perl -ane 's/y/N/ig;print;' $hairpin > hairpin_yn.fa + miRDeep2.pl \\ $reads_collapsed \\ $refgenome \\ $reads_vs_refdb \\ $mature \\ none \\ - $hairpin \\ + hairpin_yn.fa \\ -d \\ -z _${reads_collapsed.simpleName} """ From 72f16954c42fbad5972d6d2149870410e7c8e134 Mon Sep 17 00:00:00 2001 From: Lorena Pantano Date: Fri, 19 Feb 2021 09:33:38 -0500 Subject: [PATCH 087/146] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7752fbc5..a446e695 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,6 +2,8 @@ ## v1.1dev - [date] +* Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) +* Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) * Change `--reads` to `--input` for consistency with rest of nf-core * Made `CamelCase` pipeline parameters `snake_case` and lower case * `clip_R1` -> `clip_r1` From 60e1d102a7a5b47a56a9a46d8c5294ac76ba30e4 Mon Sep 17 00:00:00 2001 From: Pernilla Ericsson Date: Wed, 24 Feb 2021 09:16:10 +0100 Subject: [PATCH 088/146] Removed reference to releases before v1.0.0 and added headings for added --- CHANGELOG.md | 37 +++++++++++++++++++++++++------------ 1 file changed, 25 insertions(+), 12 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a446e695..e62a7e86 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -41,12 +41,13 @@ * Added `conda-forge::pygments=2.5.2` * Removed `conda-forge::r-markdown=1.0` -## v1.0.0 - 2019-09-19 +## [v1.0.0] - 2019-09-19 -* Add figures to output documentation -* Add samtools stats for genome alignments -* Add seqkit and remove razers -* Add mirtop and razers tools +### Added +* Figures to output documentation +* Samtools stats for genome alignments +* Seqkit and remove razers +* Mirtop and razers tools * Adapt code and docs to [nf-core](http://nf-co.re/) template * Update tools and Dockerfile/Singularity to match current template @@ -74,15 +75,27 @@ * bioconductor-edger=3.26.0 -> 3.26.5 * bioconductor-limma=3.40.0 -> 3.40.2 -## 1.0 - 2019-01-10 +## 2019-01-10 -* Add "protocol" with pre-defined settings -* Add miRTrace in the pipeline. -* Software updates: multiqc 1.6 to 1.7. +### Added +* "protocol" with pre-defined settings +* miRTrace in the pipeline. -## 1.0 - 2018-08-06 +### Software updates +* multiqc 1.6 -> 1.7. + +## 2018-08-06 + +### Added * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. -* Add option for sequencing centre in BAM file. -* Software updates: trim-galore 0.4.5 to 0.5.0; samtools 1.8 to 1.9; multiqc 1.5 to 1.6. +* Option for sequencing centre in BAM file. + +### Software updates + +* trim-galore 0.4.5 -> 0.5.0 +* samtools 1.8 -> 1.9 +* multiqc 1.5 -> 1.6. + +[v1.0.0]: https://github.com/nf-core/smrnaseq/releases/tag/1.0.0 \ No newline at end of file From 1579809de0ac8b89bcfa66aef52a9f61f369611d Mon Sep 17 00:00:00 2001 From: Pernilla Ericsson Date: Wed, 24 Feb 2021 09:40:24 +0100 Subject: [PATCH 089/146] Fixed blank lines around headings --- CHANGELOG.md | 9 ++++++--- 1 file changed, 6 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index e62a7e86..7aaaa08c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -44,6 +44,7 @@ ## [v1.0.0] - 2019-09-19 ### Added + * Figures to output documentation * Samtools stats for genome alignments * Seqkit and remove razers @@ -78,16 +79,18 @@ ## 2019-01-10 ### Added + * "protocol" with pre-defined settings * miRTrace in the pipeline. ### Software updates -* multiqc 1.6 -> 1.7. +* multiqc 1.6 -> 1.7. ## 2018-08-06 ### Added + * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. * Option for sequencing centre in BAM file. @@ -96,6 +99,6 @@ * trim-galore 0.4.5 -> 0.5.0 * samtools 1.8 -> 1.9 -* multiqc 1.5 -> 1.6. +* multiqc 1.5 -> 1.6 -[v1.0.0]: https://github.com/nf-core/smrnaseq/releases/tag/1.0.0 \ No newline at end of file +[v1.0.0]: https://github.com/nf-core/smrnaseq/releases/tag/1.0.0 From d33a480134269999229d96fa9500f70ebefbe1e6 Mon Sep 17 00:00:00 2001 From: Pernilla Ericsson Date: Wed, 24 Feb 2021 09:44:29 +0100 Subject: [PATCH 090/146] Removed trailing whitespace --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7aaaa08c..c4ecd33a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -89,7 +89,7 @@ ## 2018-08-06 -### Added +### Added * Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. From d82bac1de4b76c48d853dc7114cbc3b3b557c117 Mon Sep 17 00:00:00 2001 From: pericsson <39983934+pericsson@users.noreply.github.com> Date: Wed, 24 Feb 2021 13:18:15 +0100 Subject: [PATCH 091/146] Added inline links to release Co-authored-by: Phil Ewels --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index c4ecd33a..c6de8705 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -41,7 +41,7 @@ * Added `conda-forge::pygments=2.5.2` * Removed `conda-forge::r-markdown=1.0` -## [v1.0.0] - 2019-09-19 +## [v1.0.0](https://github.com/nf-core/smrnaseq/releases/tag/1.0.0) - 2019-09-19 ### Added From 4b3ed940cbf3a553f7d9154a8dc78029efa6046b Mon Sep 17 00:00:00 2001 From: pericsson <39983934+pericsson@users.noreply.github.com> Date: Wed, 24 Feb 2021 13:19:21 +0100 Subject: [PATCH 092/146] Removed release reference that was replaced with inline reference. Co-authored-by: Phil Ewels --- CHANGELOG.md | 2 -- 1 file changed, 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index c6de8705..ae9418de 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -100,5 +100,3 @@ * trim-galore 0.4.5 -> 0.5.0 * samtools 1.8 -> 1.9 * multiqc 1.5 -> 1.6 - -[v1.0.0]: https://github.com/nf-core/smrnaseq/releases/tag/1.0.0 From f4f4e1198dc935b289135031f4dfb025f40b6e0d Mon Sep 17 00:00:00 2001 From: pericsson <39983934+pericsson@users.noreply.github.com> Date: Wed, 24 Feb 2021 13:20:10 +0100 Subject: [PATCH 093/146] Added inline links to original pipeline and current pipeline. Co-authored-by: Phil Ewels --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ae9418de..28cd0450 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -91,7 +91,7 @@ ### Added -* Switch from SciLifeLab/NGI-smRNAseq to nf-core/smrnaseq. +* Port original pipeline [SciLifeLab/NGI-smRNAseq](https://github.com/SciLifeLab/NGI-smRNAseq) to [nf-core/smrnaseq](https://github.com/nf-core/smrnaseq). * Use Bowtie 1 instead of Bowtie 2 for the alignment to host reference genome. * Option for sequencing centre in BAM file. From 2b253fff2e145345229436a88330e03cc82cbcf7 Mon Sep 17 00:00:00 2001 From: Michael Steinbaugh Date: Wed, 24 Feb 2021 13:03:50 -0500 Subject: [PATCH 094/146] Fix "seqcluster" typo --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 342f11b6..1a7400aa 100644 --- a/README.md +++ b/README.md @@ -21,7 +21,7 @@ The pipeline is built using Nextflow, a workflow tool to run tasks across multip 1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/)) 1. Insert Size calculation - 2. Collapse reads ([`seqcsluter`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads)) + 2. Collapse reads ([`seqcluster`](https://seqcluster.readthedocs.io/mirna_annotation.html#processing-of-reads)) 3. Alignment against miRBase mature miRNA ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml)) 4. Alignment against miRBase hairpin 1. Unaligned reads from step 3 ([`Bowtie1`](http://bowtie-bio.sourceforge.net/index.shtml)) From a44ab5f83cd401f710bd435be41ade9f0511f0ad Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 8 Mar 2021 01:36:40 +0100 Subject: [PATCH 095/146] Speed up bowtie-build using multithreading --- main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index 56617a2c..f695bac8 100644 --- a/main.nf +++ b/main.nf @@ -333,7 +333,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build genome.fa genome + bowtie-build genome.fa genome --threads $(nproc) """ } } @@ -360,13 +360,13 @@ process make_bowtie_index { seqkit seq --rna2dna mature_sps.fa > mature_igenome.fa fasta_formatter -w 0 -i mature_igenome.fa -o mature_idx.fa # fasta_nucleotide_changer -d -i mature_igenome.fa -o mature_idx.fa - bowtie-build mature_idx.fa mature_idx + bowtie-build mature_idx.fa mature_idx --threads $(nproc) seqkit grep -r --pattern \".*${params.mirtrace_species}-.*\" $hairpin > hairpin_sps.fa seqkit seq --rna2dna hairpin_sps.fa > hairpin_igenome.fa # fasta_nucleotide_changer -d -i hairpin_igenome.fa -o hairpin_idx.fa fasta_formatter -w 0 -i hairpin_igenome.fa -o hairpin_idx.fa - bowtie-build hairpin_idx.fa hairpin_idx + bowtie-build hairpin_idx.fa hairpin_idx --threads $(nproc) """ } From a3846bbb5b32e790a4752a632a5f80e416348d8f Mon Sep 17 00:00:00 2001 From: Konrad Stawiski Date: Mon, 8 Mar 2021 02:10:55 +0100 Subject: [PATCH 096/146] Forgot escape char --- main.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/main.nf b/main.nf index f695bac8..051945a0 100644 --- a/main.nf +++ b/main.nf @@ -333,7 +333,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build genome.fa genome --threads $(nproc) + bowtie-build genome.fa genome --threads \$(nproc) """ } } @@ -360,13 +360,13 @@ process make_bowtie_index { seqkit seq --rna2dna mature_sps.fa > mature_igenome.fa fasta_formatter -w 0 -i mature_igenome.fa -o mature_idx.fa # fasta_nucleotide_changer -d -i mature_igenome.fa -o mature_idx.fa - bowtie-build mature_idx.fa mature_idx --threads $(nproc) + bowtie-build mature_idx.fa mature_idx --threads \$(nproc) seqkit grep -r --pattern \".*${params.mirtrace_species}-.*\" $hairpin > hairpin_sps.fa seqkit seq --rna2dna hairpin_sps.fa > hairpin_igenome.fa # fasta_nucleotide_changer -d -i hairpin_igenome.fa -o hairpin_idx.fa fasta_formatter -w 0 -i hairpin_igenome.fa -o hairpin_idx.fa - bowtie-build hairpin_idx.fa hairpin_idx --threads $(nproc) + bowtie-build hairpin_idx.fa hairpin_idx --threads \$(nproc) """ } @@ -638,7 +638,7 @@ process edgeR_mirna { process mirtop_bam_hairpin { label 'process_medium' tag "$input" - publishDir "${params.outdir}", mode: 'copy' + publishDir "${params.outdir}", mode: 'cbopy' when: mirna_gtf From 842225d51a5abb11c3662e6527f9f499d377f5b8 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 18 Mar 2021 12:05:28 +0000 Subject: [PATCH 097/146] Template update for nf-core/tools version 1.13 --- .github/.dockstore.yml | 1 + .github/CONTRIBUTING.md | 4 +- .github/ISSUE_TEMPLATE/bug_report.md | 2 +- .github/ISSUE_TEMPLATE/feature_request.md | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 6 +- .github/workflows/awsfulltest.yml | 17 +- .github/workflows/awstest.yml | 17 +- .github/workflows/branch.yml | 7 +- .github/workflows/linting.yml | 2 +- CODE_OF_CONDUCT.md | 117 ++++- Dockerfile | 6 +- README.md | 7 +- assets/email_template.html | 1 - assets/nf-core-smrnaseq_logo.png | Bin 110462 -> 18908 bytes bin/markdown_to_html.py | 0 bin/scrape_software_versions.py | 0 conf/base.config | 2 +- conf/test.config | 2 + conf/test_full.config | 2 + docs/images/nf-core-smrnaseq_logo.png | Bin 110462 -> 35978 bytes docs/output.md | 4 - docs/usage.md | 12 +- lib/Headers.groovy | 43 ++ lib/NfcoreSchema.groovy | 571 ++++++++++++++++++++++ lib/nfcore_external_java_deps.jar | Bin 0 -> 2291171 bytes main.nf | 143 ++---- nextflow.config | 42 +- nextflow_schema.json | 27 +- 28 files changed, 866 insertions(+), 171 deletions(-) mode change 100755 => 100644 bin/markdown_to_html.py mode change 100755 => 100644 bin/scrape_software_versions.py create mode 100644 lib/Headers.groovy create mode 100644 lib/NfcoreSchema.groovy create mode 100644 lib/nfcore_external_java_deps.jar diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml index 030138a0..191fabd2 100644 --- a/.github/.dockstore.yml +++ b/.github/.dockstore.yml @@ -3,3 +3,4 @@ version: 1.2 workflows: - subclass: nfl primaryDescriptorPath: /nextflow.config + publish: True diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index fc8877f8..3542a6dc 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`) +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`). 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. @@ -87,7 +87,7 @@ Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 8f1c6a52..9e7d5061 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -55,7 +55,7 @@ Have you provided the following extra information/files: ## Container engine -- Engine: +- Engine: - version: - Image tag: diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index bea7e5e9..23834e1c 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,6 +1,6 @@ --- name: Feature request -about: Suggest an idea for the nf-core website +about: Suggest an idea for the nf-core/smrnaseq pipeline labels: enhancement --- diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index bddc471c..edd0ac48 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -15,9 +15,9 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 41d5fc22..18f84c8b 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -9,6 +9,16 @@ on: types: [completed] workflow_dispatch: + +env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + + jobs: run-awstest: name: Run AWS full tests @@ -27,13 +37,6 @@ jobs: # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command - env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} run: | aws batch submit-job \ --region eu-west-1 \ diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index eeab8b33..205e94fc 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -6,6 +6,16 @@ name: nf-core AWS test on: workflow_dispatch: + +env: + AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} + AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} + TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} + AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} + AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} + AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} + + jobs: run-awstest: name: Run AWS tests @@ -23,13 +33,6 @@ jobs: # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix - env: - AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} - AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} - TOWER_ACCESS_TOKEN: ${{ secrets.AWS_TOWER_TOKEN }} - AWS_JOB_DEFINITION: ${{ secrets.AWS_JOB_DEFINITION }} - AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} - AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} run: | aws batch submit-job \ --region eu-west-1 \ diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index b59bb311..29531480 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -13,7 +13,7 @@ jobs: - name: Check PRs if: github.repository == 'nf-core/smrnaseq' run: | - { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/smrnaseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/smrnaseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure @@ -25,11 +25,12 @@ jobs: message: | Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch. + It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch. The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch. + Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. + Note that even after this, the test will continue to show as failing until you push a new commit. Thanks again for your contribution! repo-token: ${{ secrets.GITHUB_TOKEN }} diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index bef81e61..58e281df 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -69,7 +69,7 @@ jobs: if: ${{ always() }} uses: actions/upload-artifact@v2 with: - name: linting-log-file + name: linting-logs path: | lint_log.txt lint_results.md diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index 405fb1bf..f4fd052f 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,46 +1,111 @@ -# Contributor Covenant Code of Conduct +# Code of Conduct at nf-core (v1.0) ## Our Pledge -In the interest of fostering an open and welcoming environment, we as contributors and maintainers pledge to making participation in our project and our community a harassment-free experience for everyone, regardless of age, body size, disability, ethnicity, gender identity and expression, level of experience, nationality, personal appearance, race, religion, or sexual identity and orientation. +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: -## Our Standards +- Age +- Body size +- Familial status +- Gender identity and expression +- Geographical location +- Level of experience +- Nationality and national origins +- Native language +- Physical and neurological ability +- Race or ethnicity +- Religion +- Sexual identity and orientation +- Socioeconomic status -Examples of behavior that contributes to creating a positive environment include: +Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. -* Using welcoming and inclusive language -* Being respectful of differing viewpoints and experiences -* Gracefully accepting constructive criticism -* Focusing on what is best for the community -* Showing empathy towards other community members +## Preamble -Examples of unacceptable behavior by participants include: +> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. -* The use of sexualized language or imagery and unwelcome sexual attention or advances -* Trolling, insulting/derogatory comments, and personal or political attacks -* Public or private harassment -* Publishing others' private information, such as a physical or electronic address, without explicit permission -* Other conduct which could reasonably be considered inappropriate in a professional setting +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. + +nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. + +We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. + +Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. + +We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. + +Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re ## Our Responsibilities -Project maintainers are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behavior. +The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. + +The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. + +Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. + +## When are where does this Code of Conduct apply? + +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: + +- Communicating with an official project email address. +- Communicating with community members within the nf-core Slack channel. +- Participating in hackathons organised by nf-core (both online and in-person events). +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Representing nf-core on social media. This includes both official and personal accounts. + +## nf-core cares 😊 + +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): + +- Ask for consent before sharing another community member’s personal information (including photographs) on social media. +- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. +- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) +- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) +- Focus on what is best for the team and the community. (When in doubt, ask) +- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Take breaks when you feel like you need them. +- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) + +## nf-core frowns on 😕 + +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. + +- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. +- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. +- Spamming or trolling of individuals on social media. +- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. + +### Online Trolling + +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. + +All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. + +## Procedures for Reporting CoC violations -Project maintainers have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -## Scope +You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). -This Code of Conduct applies both within project spaces and in public spaces when an individual is representing the project or its community. Examples of representing a project or community include using an official project e-mail address, posting via an official social media account, or acting as an appointed representative at an online or offline event. Representation of a project may be further defined and clarified by project maintainers. +Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. -## Enforcement +All reports will be handled with utmost discretion and confidentially. -Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-co.re/join/slack). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately. +## Attribution and Acknowledgements -Project maintainers who do not follow or enforce the Code of Conduct in good faith may face temporary or permanent repercussions as determined by other members of the project's leadership. +- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) +- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) +- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) +- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) -## Attribution +## Changelog -This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version] +### v1.0 - March 12th, 2021 -[homepage]: https://contributor-covenant.org -[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/ +- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/Dockerfile b/Dockerfile index 42c3b807..309d6a1e 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.12.1 +FROM nfcore/base:1.13 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" @@ -11,7 +11,3 @@ ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1dev/bin:$PATH # Dump the details of the installed packages to a file for posterity RUN conda env export --name nf-core-smrnaseq-1.1dev > nf-core-smrnaseq-1.1dev.yml - -# Instruct R processes to use these empty files instead of clashing with a local version -RUN touch .Rprofile -RUN touch .Renviron diff --git a/README.md b/README.md index 141fa267..70516c0d 100644 --- a/README.md +++ b/README.md @@ -21,12 +21,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool 1. Install [`nextflow`](https://nf-co.re/usage/installation) -2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) or [`Podman`](https://podman.io/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ +2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ 3. Download the pipeline and test it on a minimal dataset with a single command: ```bash - nextflow run nf-core/smrnaseq -profile test, + nextflow run nf-core/smrnaseq -profile test, ``` > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. @@ -36,7 +36,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ```bash - nextflow run nf-core/smrnaseq -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + nextflow run nf-core/smrnaseq -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 ``` See [usage docs](https://nf-co.re/smrnaseq/usage) for all of the available options when running the pipeline. @@ -83,7 +83,6 @@ You can cite the `nf-core` publication as follows: > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). -> ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) In addition, references of tools and data used in this pipeline are as follows: diff 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zm9>zrtK?2~r0Y8A78;hE)=xx{nYj*`v-sg#(;Y<7ATdL$VDw1{3QB8Zi+Bk4#~9p? zF`ZW(J&LpX@Gkty#?;Kw<1~1^iaL8I7aHXC>%2$XGwUqV(br6!XPe_#TK^*5!m!q1 zN(-?}>s3^;-be)&_NLm5cA>p~_UdJcTGg>Jk^@(4kj6V{z6+IU;|BG(uJO%_B!xuY{y|M4YuR(p%{Ko z9DXaC?Zy9!z_^J3vHru>79SgEb5_8*yFLDI5$O1fCHbT9HPrXbTZahn5A>@$5qa&u D_{`Xn diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py old mode 100755 new mode 100644 diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py old mode 100755 new mode 100644 diff --git a/conf/base.config b/conf/base.config index e208748f..2d268215 100644 --- a/conf/base.config +++ b/conf/base.config @@ -47,5 +47,5 @@ process { withName:get_software_versions { cache = false } - + } diff --git a/conf/test.config b/conf/test.config index d41aecd4..e2746fa6 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,4 +23,6 @@ params { ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] ] + // Ignore `--input` as otherwise the parameter validation will throw an error + schema_ignore_params = 'genomes,input_paths,input' } diff --git a/conf/test_full.config b/conf/test_full.config index f06cc1e2..fca9187d 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -19,4 +19,6 @@ params { ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] ] + 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command for running the pipeline is as follows: - - nextflow run nf-core/smrnaseq --input '*_R{1,2}.fastq.gz' -profile docker - - Mandatory arguments: - --input [file] Path to input data (must be surrounded with quotes) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more - - Options: - --genome [str] Name of iGenomes reference - --single_end [bool] Specifies that the input is single-end reads - - References If not specified in the configuration file or you wish to overwrite any of the references - --fasta [file] Path to fasta reference - - Other options: - --outdir [file] The output directory where the results will be saved - --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) - --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic - - AWSBatch options: - --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion [str] The AWS Region for your AWS Batch job to run on - --awscli [str] Path to the AWS CLI tool - """.stripIndent() -} +log.info Headers.nf_core(workflow, params.monochrome_logs) -// Show help message +//////////////////////////////////////////////////// +/* -- PRINT HELP -- */ +////////////////////////////////////////////////////+ +def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { - helpMessage() + def command = "nextflow run nf-core/smrnaseq --input '*_R{1,2}.fastq.gz' -profile docker" + log.info NfcoreSchema.params_help(workflow, params, json_schema, command) exit 0 } -/* - * SET UP CONFIGURATION VARIABLES - */ +//////////////////////////////////////////////////// +/* -- VALIDATE PARAMETERS -- */ +////////////////////////////////////////////////////+ +if (params.validate_params) { + NfcoreSchema.validateParameters(params, json_schema, log) +} + +//////////////////////////////////////////////////// +/* -- Collect configuration parameters -- */ +//////////////////////////////////////////////////// // Check if genome exists in the config file if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}" } // TODO nf-core: Add any reference files that are needed @@ -73,22 +48,15 @@ if (params.genomes && params.genome && !params.genomes.containsKey(params.genome params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -custom_runName = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - custom_runName = workflow.runName -} - // Check AWS batch settings if (workflow.profile.contains('awsbatch')) { // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!' // Check outdir paths to be S3 buckets if running on AWSBatch // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!' // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.' } // Stage config files @@ -105,13 +73,13 @@ if (params.input_paths) { Channel .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } .into { ch_read_files_fastqc; ch_read_files_trimming } } else { Channel .from(params.input_paths) .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } + .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } .into { ch_read_files_fastqc; ch_read_files_trimming } } } else { @@ -121,11 +89,15 @@ if (params.input_paths) { .into { ch_read_files_fastqc; ch_read_files_trimming } } +//////////////////////////////////////////////////// +/* -- PRINT PARAMETER SUMMARY -- */ +//////////////////////////////////////////////////// +log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) + // Header log info -log.info nfcoreHeader() def summary = [:] if (workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Run Name'] = custom_runName ?: workflow.runName +summary['Run Name'] = workflow.runName // TODO nf-core: Report custom parameters here summary['Input'] = params.input summary['Fasta Ref'] = params.fasta @@ -152,8 +124,6 @@ if (params.email || params.email_on_fail) { summary['E-mail on failure'] = params.email_on_fail summary['MultiQC maxsize'] = params.max_multiqc_email_size } -log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") -log.info "-\033[2m--------------------------------------------------\033[0m-" // Check the hostnames against configured profiles checkHostname() @@ -180,13 +150,13 @@ Channel.from(summary.collect{ [it.key, it.value] }) process get_software_versions { publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, saveAs: { filename -> - if (filename.indexOf(".csv") > 0) filename + if (filename.indexOf('.csv') > 0) filename else null - } + } output: file 'software_versions_mqc.yaml' into ch_software_versions_yaml - file "software_versions.csv" + file 'software_versions.csv' script: // TODO nf-core: Get all tools to print their version number here @@ -207,14 +177,14 @@ process fastqc { label 'process_medium' publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, saveAs: { filename -> - filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" - } + filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename" + } input: set val(name), file(reads) from ch_read_files_fastqc output: - file "*_fastqc.{zip,html}" into ch_fastqc_results + file '*_fastqc.{zip,html}' into ch_fastqc_results script: """ @@ -242,8 +212,12 @@ process multiqc { file "multiqc_plots" script: - rtitle = custom_runName ? "--title \"$custom_runName\"" : '' - rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' + rtitle = '' + rfilename = '' + if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + rtitle = "--title \"${workflow.runName}\"" + rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" + } custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ @@ -262,7 +236,7 @@ process output_documentation { file images from ch_output_docs_images output: - file "results_description.html" + file 'results_description.html' script: """ @@ -282,7 +256,7 @@ workflow.onComplete { } def email_fields = [:] email_fields['version'] = workflow.manifest.version - email_fields['runName'] = custom_runName ?: workflow.runName + email_fields['runName'] = workflow.runName email_fields['success'] = workflow.success email_fields['dateComplete'] = workflow.complete email_fields['duration'] = workflow.duration @@ -389,28 +363,9 @@ workflow.onComplete { } - -def nfcoreHeader() { - // Log colors ANSI codes - c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_white = params.monochrome_logs ? '' : "\033[0;37m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - - return """ -${c_dim}--------------------------------------------------${c_reset}- - ${c_green},--.${c_black}/${c_green},-.${c_reset} - ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} - ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} - ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} - ${c_green}`._,._,\'${c_reset} - ${c_purple} nf-core/smrnaseq v${workflow.manifest.version}${c_reset} - -${c_dim}--------------------------------------------------${c_reset}- - """.stripIndent() +workflow.onError { + // Print unexpected parameters - easiest is to just rerun validation + NfcoreSchema.validateParameters(params, json_schema, log) } def checkHostname() { @@ -419,15 +374,15 @@ def checkHostname() { def c_red = params.monochrome_logs ? '' : "\033[1;91m" def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" if (params.hostnames) { - def hostname = "hostname".execute().text.trim() + def hostname = 'hostname'.execute().text.trim() params.hostnames.each { prof, hnames -> hnames.each { hname -> if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "====================================================\n" + + log.error '====================================================\n' + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "============================================================" + '============================================================' } } } diff --git a/nextflow.config b/nextflow.config index cdc3894c..80a90c08 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,13 +11,13 @@ params { // Workflow flags // TODO nf-core: Specify your pipeline's command line flags genome = false - input = "data/*{1,2}.fastq.gz" + input = null + input_paths = null single_end = false outdir = './results' publish_dir_mode = 'copy' // Boilerplate options - name = false multiqc_config = false email = false email_on_fail = false @@ -34,6 +34,9 @@ params { config_profile_description = false config_profile_contact = false config_profile_url = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,input_paths' // Defaults only, expecting to be overwritten max_memory = 128.GB @@ -57,10 +60,21 @@ try { } profiles { - conda { process.conda = "$projectDir/environment.yml" } + conda { + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud = false + process.conda = "$projectDir/environment.yml" + } debug { process.beforeScript = 'echo $HOSTNAME' } docker { docker.enabled = true + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false // Avoid this error: // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 @@ -68,11 +82,33 @@ profiles { docker.runOptions = '-u \$(id -u):\$(id -g)' } singularity { + docker.enabled = false singularity.enabled = true + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false singularity.autoMounts = true } podman { + singularity.enabled = false + docker.enabled = false podman.enabled = true + shifter.enabled = false + charliecloud = false + } + shifter { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = true + charliecloud.enabled = false + } + charliecloud { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = true } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } diff --git a/nextflow_schema.json b/nextflow_schema.json index 55101fc8..b9e417f6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -104,12 +104,12 @@ "move" ] }, - "name": { - "type": "string", - "description": "Workflow name.", - "fa_icon": "fas fa-fingerprint", - "hidden": true, - "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true }, "email_on_fail": { "type": "string", @@ -153,6 +153,13 @@ "default": "${params.outdir}/pipeline_info", "fa_icon": "fas fa-cogs", "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." } } }, @@ -176,6 +183,7 @@ "description": "Maximum amount of memory that can be requested for any single job.", "default": "128.GB", "fa_icon": "fas fa-memory", + "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$", "hidden": true, "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" }, @@ -184,6 +192,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", + "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } @@ -218,6 +227,12 @@ "hidden": true, "fa_icon": "fas fa-users-cog" }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, "config_profile_description": { "type": "string", "description": "Institutional config description.", From b5b51dd6abab8451721e4ae17d05a926d4d7a9b3 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 19 Mar 2021 13:07:31 +0000 Subject: [PATCH 098/146] Template update for nf-core/tools version 1.13.1 --- .github/workflows/branch.yml | 12 ++++++++++-- Dockerfile | 2 +- 2 files changed, 11 insertions(+), 3 deletions(-) diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 29531480..0ff3e0f5 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -23,11 +23,19 @@ jobs: uses: mshick/add-pr-comment@v1 with: message: | + ## This PR is against the `master` branch :x: + + * Do not close this PR + * Click _Edit_ and change the `base` to `dev` + * This CI test will remain failed until you push a new commit + + --- + Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the ${{github.event.pull_request.base.repo.full_name }} `master` branch. + It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch. The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.base.repo.full_name }} `dev` branch. + Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. Note that even after this, the test will continue to show as failing until you push a new commit. diff --git a/Dockerfile b/Dockerfile index 309d6a1e..46f1edc8 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13 +FROM nfcore/base:1.13.1 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" From 9bef1e1a90d29bd3fba65d2d7661531a2e024493 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 23 Mar 2021 16:35:00 +0000 Subject: [PATCH 099/146] Template update for nf-core/tools version 1.13.2 --- .github/workflows/ci.yml | 2 +- .github/workflows/linting.yml | 57 ++++++++++++++++++++++++++++++++- Dockerfile | 2 +- bin/markdown_to_html.py | 0 bin/scrape_software_versions.py | 0 lib/NfcoreSchema.groovy | 2 +- 6 files changed, 59 insertions(+), 4 deletions(-) mode change 100644 => 100755 bin/markdown_to_html.py mode change 100644 => 100755 bin/scrape_software_versions.py diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 20147c26..42dbf66f 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', ''] + nxf_ver: ['20.04.0', '21.03.0-edge'] steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 58e281df..fcde400c 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -19,6 +19,34 @@ jobs: run: npm install -g markdownlint-cli - name: Run Markdownlint run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## Markdown linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `markdownlint-cli` + * On Mac: `brew install markdownlint-cli` + * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) + * Fix the markdown errors + * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` + * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + YAML: runs-on: ubuntu-latest steps: @@ -29,7 +57,34 @@ jobs: - name: Install yaml-lint run: npm install -g yaml-lint - name: Run yaml-lint - run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml") + run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml") + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## YAML linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `yaml-lint` + * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`) + * Fix the markdown errors + * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")` + * Fix any reported errors in your YAML files + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + nf-core: runs-on: ubuntu-latest steps: diff --git a/Dockerfile b/Dockerfile index 46f1edc8..5ff55649 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.1 +FROM nfcore/base:1.13.2 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" diff --git a/bin/markdown_to_html.py b/bin/markdown_to_html.py old mode 100644 new mode 100755 diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py old mode 100644 new mode 100755 diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 78e8c65d..54935ec8 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -219,7 +219,7 @@ class NfcoreSchema { } } } - if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) { + if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) { rawSchema.get("properties").remove(ignore_param) } if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { From f74d4caf78f829ac7a2342a37ff2c9ec58e22dd9 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 25 Mar 2021 18:17:29 +0000 Subject: [PATCH 100/146] Template update for nf-core/tools version 1.13.3 --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 5ff55649..46fe4be6 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.2 +FROM nfcore/base:1.13.3 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" From e0ac01db2e438db289c36547dc0dbe0cf8046470 Mon Sep 17 00:00:00 2001 From: Erik Danielsson Date: Tue, 13 Apr 2021 10:21:23 +0200 Subject: [PATCH 101/146] Fix lint test: 'files_unchanged' --- .github/PULL_REQUEST_TEMPLATE.md | 7 +++--- assets/email_template.txt | 6 ++--- lib/NfcoreSchema.groovy | 42 +++++++++++++++----------------- nextflow.config | 2 +- 4 files changed, 26 insertions(+), 31 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index edd0ac48..e74762ec 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,14 +10,15 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) --> + ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/assets/email_template.txt b/assets/email_template.txt index 86f988ef..6e8b6393 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -35,8 +35,6 @@ Pipeline Configuration: ----------------------- <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> - -- -nf-core/smrnaseq is a bioinformatics best-practice analysis pipeline used for small RNA sequencing data at the National Genomics Infrastructure at SciLifeLab Stockholm, Sweden. -The pipeline uses Nextflow, a bioinformatics workflow tool. It pre-processes raw data from FastQ inputs, aligns the reads and performs extensive quality-control on the results. -For more information, please see the pipeline homepage: https://github.com/nf-core/smrnaseq +nf-core/smrnaseq +https://github.com/nf-core/smrnaseq diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 54935ec8..adfb2aec 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -191,11 +191,11 @@ class NfcoreSchema { // Remove an element from a JSONArray private static JSONArray removeElement(jsonArray, element){ - def list = [] + def list = [] int len = jsonArray.length() - for (int i=0;i Date: Tue, 13 Apr 2021 10:36:54 +0200 Subject: [PATCH 102/146] Fix lint test failing due to 'single_end' missing in schema --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index a00fe2c1..de645b43 100644 --- a/nextflow.config +++ b/nextflow.config @@ -57,7 +57,7 @@ params { publish_dir_mode = 'copy' validate_params = true show_hidden_params = false - schema_ignore_params = 'genomes,input_paths' + schema_ignore_params = 'genomes,input_paths,single_end' // Defaults only, expecting to be overwritten max_memory = 128.GB From 1ab5a5c2ca22070a6fd1d5c38fd7f98fb1f4c523 Mon Sep 17 00:00:00 2001 From: Erik Danielsson Date: Tue, 13 Apr 2021 10:48:57 +0200 Subject: [PATCH 103/146] Fix failing lint tests 'files_unchanged' --- .github/PULL_REQUEST_TEMPLATE.md | 7 +++--- lib/NfcoreSchema.groovy | 42 +++++++++++++++++--------------- 2 files changed, 26 insertions(+), 23 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index e74762ec..edd0ac48 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,15 +10,14 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) --> - ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index adfb2aec..54935ec8 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -191,11 +191,11 @@ class NfcoreSchema { // Remove an element from a JSONArray private static JSONArray removeElement(jsonArray, element){ - def list = [] + def list = [] int len = jsonArray.length() - for (int i=0;i Date: Tue, 13 Apr 2021 13:39:28 +0200 Subject: [PATCH 104/146] Remove redundant TODOs --- README.md | 3 --- nextflow.config | 1 - 2 files changed, 4 deletions(-) diff --git a/README.md b/README.md index 351d7104..49b5bcd6 100644 --- a/README.md +++ b/README.md @@ -88,6 +88,3 @@ You can cite the `nf-core` publication as follows: > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). In addition, references of tools and data used in this pipeline are as follows: - - - diff --git a/nextflow.config b/nextflow.config index de645b43..df30028b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -10,7 +10,6 @@ params { // Workflow flags input = "data/*.fastq.gz" - // TODO nf-core: Specify your pipeline's command line flags input_paths = null single_end = false outdir = './results' From 353cd3825da8fd5e36391140a8cbe690b7c0d3d8 Mon Sep 17 00:00:00 2001 From: Erik Danielsson Date: Wed, 14 Apr 2021 09:40:29 +0200 Subject: [PATCH 105/146] Remove 'single_end' and add 'custom_config_profile' --- nextflow.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index df30028b..cd49d904 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,7 +11,6 @@ params { // Workflow flags input = "data/*.fastq.gz" input_paths = null - single_end = false outdir = './results' protocol = 'illumina' @@ -50,13 +49,14 @@ params { custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false + config_profile_name = "" config_profile_description = false config_profile_contact = false config_profile_url = false publish_dir_mode = 'copy' validate_params = true show_hidden_params = false - schema_ignore_params = 'genomes,input_paths,single_end' + schema_ignore_params = 'genomes,input_paths' // Defaults only, expecting to be overwritten max_memory = 128.GB From 6f01165fe3d7e37abf8940dce3388398908ba841 Mon Sep 17 00:00:00 2001 From: Erik Danielsson Date: Wed, 14 Apr 2021 09:48:03 +0200 Subject: [PATCH 106/146] Empty string -> null --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index cd49d904..6b0bcf2d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -49,7 +49,7 @@ params { custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false - config_profile_name = "" + config_profile_name = null config_profile_description = false config_profile_contact = false config_profile_url = false From 9164e60f8541cfa8a976c0de9976962f61da7e2a Mon Sep 17 00:00:00 2001 From: Erik Danielsson Date: Wed, 14 Apr 2021 14:24:33 +0200 Subject: [PATCH 107/146] Add Phil's suggestions --- main.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/main.nf b/main.nf index 60dbade7..e521f859 100644 --- a/main.nf +++ b/main.nf @@ -333,7 +333,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build genome.fa genome --threads \$(nproc) + bowtie-build genome.fa genome --threads \$(task.cpus) """ } } @@ -360,13 +360,13 @@ process make_bowtie_index { seqkit seq --rna2dna mature_sps.fa > mature_igenome.fa fasta_formatter -w 0 -i mature_igenome.fa -o mature_idx.fa # fasta_nucleotide_changer -d -i mature_igenome.fa -o mature_idx.fa - bowtie-build mature_idx.fa mature_idx --threads \$(nproc) + bowtie-build mature_idx.fa mature_idx --threads \$(task.cpus) seqkit grep -r --pattern \".*${params.mirtrace_species}-.*\" $hairpin > hairpin_sps.fa seqkit seq --rna2dna hairpin_sps.fa > hairpin_igenome.fa # fasta_nucleotide_changer -d -i hairpin_igenome.fa -o hairpin_idx.fa fasta_formatter -w 0 -i hairpin_igenome.fa -o hairpin_idx.fa - bowtie-build hairpin_idx.fa hairpin_idx --threads \$(nproc) + bowtie-build hairpin_idx.fa hairpin_idx --threads \$(task.cpus) """ } @@ -638,7 +638,7 @@ process edgeR_mirna { process mirtop_bam_hairpin { label 'process_medium' tag "$input" - publishDir "${params.outdir}", mode: 'cbopy' + publishDir "${params.outdir}", mode: 'copy' when: mirna_gtf @@ -841,7 +841,7 @@ process mirdeep2 { script: """ perl -ane 's/y/N/ig;print;' $hairpin > hairpin_yn.fa - + miRDeep2.pl \\ $reads_collapsed \\ $refgenome \\ From 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z@rNk8WQY(7keEBR7Q|VDrwRPjhj**Wx^$RILt?~HJ$@x}dNuF0Ygk3X5$_lwmO!upRQ!PQ=q$xvm zKwBLJdK)h=8SLOwcps87TKKscHl&!Qh8q8QNMt9Xf29}w)J|N7byf^qN$5%c#&OsR}6GJ_vIx}r8X zjQ)O$kO4oB8LvN$8X!K^K!x)^EL(I7%mt|iJRMM&o)b|QrLU5jnl=WVOX4?}hao|X z&7Aw`tIa$<+&<}oRl5Q>wHC4V@i3$f)2s=-*I{Q=g0v|vO;`ULZuYrdjha`217fyWu?b8AB@`!gmS+9!PSeYe+`D{Bl|Lo z8y8@Bg#&2hv9~A6e)@TFtK({h&><@TZj6POKaij)NlC$HM-`^SSa82S#*lBWM8GEt1QQk&(2(`wyXMtsA<@)BQ3Dq1 zH2Yd85rBx~=EcqD@l|u3;P{+aFFk&rKxRa8lIy`$M;$XD{97ylcpi8c`;m4=f#vBZ zq9Y(s`b9%;e-)|AUbG)_0u(dfE6GEscjx;u*QPZdMm(!U$P;U&*(lJ0(t%K=C{r9ZilLhzVXU{%zb#Zyoq<H(R1 zoB8aIPyWcbw(`ufm3RR7wZkjoB*}E}Yt_>0qu=fD!uh{Tzpm|Pk^9f@3jU$1T>ACj zAN=p*`R}6m?^eJQ_-~|Oky}~_jQ_^R|Eu)za&eWWb - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - image/svg+xml - - - - - - - A small-RNA sequencing analysis pipeline - smrnaseq - - - - - - - - - - - - - - - - - - - - - - - - - From ad2ff33f85a5d85c584b74922b5d84999030bd3c Mon Sep 17 00:00:00 2001 From: Sarah Djebali Quelen Date: Tue, 4 May 2021 11:03:14 +0200 Subject: [PATCH 109/146] remove space in genome header and special nt in hairpin for mirdeep2 to work --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 56617a2c..47c2829e 100644 --- a/main.nf +++ b/main.nf @@ -840,7 +840,7 @@ process mirdeep2 { script: """ - perl -ane 's/y/N/ig;print;' $hairpin > hairpin_yn.fa + awk '{ gsub(/Y/,"N",\$0); gsub(/B/,"N",\$0); gsub(/K/,"N",\$0); gsub(/M/,"N",\$0); gsub(/R/,"N",\$0); gsub(/S/,"N",\$0); gsub(/W/,"N",\$0); print}' $hairpin > hairpin_ok.fa miRDeep2.pl \\ $reads_collapsed \\ @@ -848,7 +848,7 @@ process mirdeep2 { $reads_vs_refdb \\ $mature \\ none \\ - hairpin_yn.fa \\ + hairpin_ok.fa \\ -d \\ -z _${reads_collapsed.simpleName} """ From 58f6b681d9255a7d551c6fc478b91fa411f63d23 Mon Sep 17 00:00:00 2001 From: Sarah Djebali Quelen Date: Tue, 4 May 2021 14:13:27 +0200 Subject: [PATCH 110/146] adding changes made to CHANGELOG.md --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 28cd0450..ee60af4f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,6 +2,7 @@ ## v1.1dev - [date] +* remove spaces in genome headers and replace special nt by N in hairpin file for mirdeep2 to work. Follow up from [[#69]] (https://github.com/nf-core/smrnaseq/pull/79) * Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) * Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) * Change `--reads` to `--input` for consistency with rest of nf-core From be31fec5eb86a88e52f1a34ec127fd9bafaeffd3 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Thu, 6 May 2021 11:48:50 +0200 Subject: [PATCH 111/146] install libtbb2 in container --- Dockerfile | 3 +++ 1 file changed, 3 insertions(+) diff --git a/Dockerfile b/Dockerfile index 46fe4be6..e0c4ed6e 100644 --- a/Dockerfile +++ b/Dockerfile @@ -2,6 +2,9 @@ FROM nfcore/base:1.13.3 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" +# Install libtbb2 package for bowtie +RUN apt-get update && apt-get install libtbb2 -y + # Install the conda environment COPY environment.yml / RUN conda env create --quiet -f /environment.yml && conda clean -a From 4f579e4c908cf8ebe6850001b3741fda80e10f9a Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 6 May 2021 12:56:23 +0200 Subject: [PATCH 112/146] Update .github/workflows/ci.yml --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 4bb81ec6..d179cbc0 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', '21.03.0-edge'] + nxf_ver: ['20.04.0', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 From a2eea2e95cbfd1614a6ae8441106ea712ce66e2e Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 6 May 2021 13:44:38 +0200 Subject: [PATCH 113/146] Apply suggestions from code review --- main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index 303177a4..a14e7ea5 100644 --- a/main.nf +++ b/main.nf @@ -290,7 +290,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build genome.fa genome --threads \$(task.cpus) + bowtie-build genome.fa genome --threads ${task.cpus} """ } } @@ -317,13 +317,13 @@ process make_bowtie_index { seqkit seq --rna2dna mature_sps.fa > mature_igenome.fa fasta_formatter -w 0 -i mature_igenome.fa -o mature_idx.fa # fasta_nucleotide_changer -d -i mature_igenome.fa -o mature_idx.fa - bowtie-build mature_idx.fa mature_idx --threads \$(task.cpus) + bowtie-build mature_idx.fa mature_idx --threads ${task.cpus} seqkit grep -r --pattern \".*${params.mirtrace_species}-.*\" $hairpin > hairpin_sps.fa seqkit seq --rna2dna hairpin_sps.fa > hairpin_igenome.fa # fasta_nucleotide_changer -d -i hairpin_igenome.fa -o hairpin_idx.fa fasta_formatter -w 0 -i hairpin_igenome.fa -o hairpin_idx.fa - bowtie-build hairpin_idx.fa hairpin_idx --threads \$(task.cpus) + bowtie-build hairpin_idx.fa hairpin_idx --threads ${task.cpus} """ } From e750145bc1b017baf8a963f0c006d3da60b3b1fd Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 6 May 2021 15:05:26 +0200 Subject: [PATCH 114/146] Revert to use a perl regex, but with additional characters --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 47c2829e..882ae0c6 100644 --- a/main.nf +++ b/main.nf @@ -840,8 +840,8 @@ process mirdeep2 { script: """ - awk '{ gsub(/Y/,"N",\$0); gsub(/B/,"N",\$0); gsub(/K/,"N",\$0); gsub(/M/,"N",\$0); gsub(/R/,"N",\$0); gsub(/S/,"N",\$0); gsub(/W/,"N",\$0); print}' $hairpin > hairpin_ok.fa - + perl -ane 's/[ybkmrsw]/N/ig;print;' $hairpin > hairpin_ok.fa + miRDeep2.pl \\ $reads_collapsed \\ $refgenome \\ From 111acd508161f0004d81568094398f9e4f3216bb Mon Sep 17 00:00:00 2001 From: Sarah Djebali Quelen Date: Thu, 6 May 2021 15:35:56 +0200 Subject: [PATCH 115/146] lint generated files --- .github/.dockstore.yml | 1 + .github/CONTRIBUTING.md | 4 +- .github/ISSUE_TEMPLATE/bug_report.md | 2 +- .github/ISSUE_TEMPLATE/feature_request.md | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 6 +- .github/workflows/branch.yml | 15 ++- .github/workflows/linting.yml | 59 ++++++++++- CODE_OF_CONDUCT.md | 117 +++++++++++++++++----- assets/email_template.html | 1 - assets/email_template.txt | 6 +- assets/nf-core-smrnaseq_logo.png | Bin 110462 -> 18908 bytes docs/images/nf-core-smrnaseq_logo.png | Bin 110462 -> 35978 bytes 12 files changed, 170 insertions(+), 43 deletions(-) diff --git a/.github/.dockstore.yml b/.github/.dockstore.yml index 030138a0..191fabd2 100644 --- a/.github/.dockstore.yml +++ b/.github/.dockstore.yml @@ -3,3 +3,4 @@ version: 1.2 workflows: - subclass: nfl primaryDescriptorPath: /nextflow.config + publish: True diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index fc8877f8..3542a6dc 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`) +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`). 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. @@ -87,7 +87,7 @@ Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md index 8f1c6a52..9e7d5061 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ b/.github/ISSUE_TEMPLATE/bug_report.md @@ -55,7 +55,7 @@ Have you provided the following extra information/files: ## Container engine -- Engine: +- Engine: - version: - Image tag: diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md index bea7e5e9..23834e1c 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ b/.github/ISSUE_TEMPLATE/feature_request.md @@ -1,6 +1,6 @@ --- name: Feature request -about: Suggest an idea for the nf-core website +about: Suggest an idea for the nf-core/smrnaseq pipeline labels: enhancement --- diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index bddc471c..edd0ac48 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -15,9 +15,9 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrn - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index b59bb311..0ff3e0f5 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -13,7 +13,7 @@ jobs: - name: Check PRs if: github.repository == 'nf-core/smrnaseq' run: | - { [[ ${{github.event.pull_request.head.repo.full_name}} == nf-core/smrnaseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/smrnaseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure @@ -23,13 +23,22 @@ jobs: uses: mshick/add-pr-comment@v1 with: message: | + ## This PR is against the `master` branch :x: + + * Do not close this PR + * Click _Edit_ and change the `base` to `dev` + * This CI test will remain failed until you push a new commit + + --- + Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the ${{github.event.pull_request.head.repo.full_name}} `master` branch. + It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch. The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the ${{github.event.pull_request.head.repo.full_name}} `dev` branch. + Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. + Note that even after this, the test will continue to show as failing until you push a new commit. Thanks again for your contribution! repo-token: ${{ secrets.GITHUB_TOKEN }} diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index bef81e61..fcde400c 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -19,6 +19,34 @@ jobs: run: npm install -g markdownlint-cli - name: Run Markdownlint run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## Markdown linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `markdownlint-cli` + * On Mac: `brew install markdownlint-cli` + * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) + * Fix the markdown errors + * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` + * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up markdownlint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + YAML: runs-on: ubuntu-latest steps: @@ -29,7 +57,34 @@ jobs: - name: Install yaml-lint run: npm install -g yaml-lint - name: Run yaml-lint - run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml") + run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml") + + # If the above check failed, post a comment on the PR explaining the failure + - name: Post PR comment + if: failure() + uses: mshick/add-pr-comment@v1 + with: + message: | + ## YAML linting is failing + + To keep the code consistent with lots of contributors, we run automated code consistency checks. + To fix this CI test, please run: + + * Install `yaml-lint` + * [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`) + * Fix the markdown errors + * Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")` + * Fix any reported errors in your YAML files + + Once you push these changes the test should pass, and you can hide this comment :+1: + + We highly recommend setting up yaml-lint in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + + Thanks again for your contribution! + repo-token: ${{ secrets.GITHUB_TOKEN }} + allow-repeats: false + + nf-core: runs-on: ubuntu-latest steps: @@ -69,7 +124,7 @@ jobs: if: ${{ always() }} uses: actions/upload-artifact@v2 with: - name: linting-log-file + name: linting-logs path: | lint_log.txt lint_results.md diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index 405fb1bf..f4fd052f 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,46 +1,111 @@ -# Contributor Covenant Code of Conduct +# Code of Conduct at nf-core (v1.0) ## Our Pledge -In the interest of fostering an open and welcoming environment, we as contributors and maintainers pledge to making participation in our project and our community a harassment-free experience for everyone, regardless of age, body size, disability, ethnicity, gender identity and expression, level of experience, nationality, personal appearance, race, religion, or sexual identity and orientation. +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: -## Our Standards +- Age +- Body size +- Familial status +- Gender identity and expression +- Geographical location +- Level of experience +- Nationality and national origins +- Native language +- Physical and neurological ability +- Race or ethnicity +- Religion +- Sexual identity and orientation +- Socioeconomic status -Examples of behavior that contributes to creating a positive environment include: +Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. -* Using welcoming and inclusive language -* Being respectful of differing viewpoints and experiences -* Gracefully accepting constructive criticism -* Focusing on what is best for the community -* Showing empathy towards other community members +## Preamble -Examples of unacceptable behavior by participants include: +> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. -* The use of sexualized language or imagery and unwelcome sexual attention or advances -* Trolling, insulting/derogatory comments, and personal or political attacks -* Public or private harassment -* Publishing others' private information, such as a physical or electronic address, without explicit permission -* Other conduct which could reasonably be considered inappropriate in a professional setting +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. + +nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. + +We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. + +Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. + +We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. + +Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re ## Our Responsibilities -Project maintainers are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behavior. +The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. + +The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. + +Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. + +## When are where does this Code of Conduct apply? + +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: + +- Communicating with an official project email address. +- Communicating with community members within the nf-core Slack channel. +- Participating in hackathons organised by nf-core (both online and in-person events). +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Representing nf-core on social media. This includes both official and personal accounts. + +## nf-core cares 😊 + +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): + +- Ask for consent before sharing another community member’s personal information (including photographs) on social media. +- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. +- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) +- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) +- Focus on what is best for the team and the community. (When in doubt, ask) +- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Take breaks when you feel like you need them. +- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) + +## nf-core frowns on 😕 + +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. + +- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. +- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. +- Spamming or trolling of individuals on social media. +- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. + +### Online Trolling + +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. + +All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. + +## Procedures for Reporting CoC violations -Project maintainers have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -## Scope +You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). -This Code of Conduct applies both within project spaces and in public spaces when an individual is representing the project or its community. Examples of representing a project or community include using an official project e-mail address, posting via an official social media account, or acting as an appointed representative at an online or offline event. Representation of a project may be further defined and clarified by project maintainers. +Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. -## Enforcement +All reports will be handled with utmost discretion and confidentially. -Instances of abusive, harassing, or otherwise unacceptable behavior may be reported by contacting the project team on [Slack](https://nf-co.re/join/slack). The project team will review and investigate all complaints, and will respond in a way that it deems appropriate to the circumstances. The project team is obligated to maintain confidentiality with regard to the reporter of an incident. Further details of specific enforcement policies may be posted separately. +## Attribution and Acknowledgements -Project maintainers who do not follow or enforce the Code of Conduct in good faith may face temporary or permanent repercussions as determined by other members of the project's leadership. +- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) +- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) +- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) +- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) -## Attribution +## Changelog -This Code of Conduct is adapted from the [Contributor Covenant][homepage], version 1.4, available at [https://www.contributor-covenant.org/version/1/4/code-of-conduct/][version] +### v1.0 - March 12th, 2021 -[homepage]: https://contributor-covenant.org -[version]: https://www.contributor-covenant.org/version/1/4/code-of-conduct/ +- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/assets/email_template.html b/assets/email_template.html index 852ea84a..e75e86ac 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,6 +1,5 @@ - diff --git a/assets/email_template.txt b/assets/email_template.txt index 86f988ef..6e8b6393 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -35,8 +35,6 @@ Pipeline Configuration: ----------------------- <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> - -- -nf-core/smrnaseq is a bioinformatics best-practice analysis pipeline used for small RNA sequencing data at the National Genomics Infrastructure at SciLifeLab Stockholm, Sweden. -The pipeline uses Nextflow, a bioinformatics workflow tool. 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z9DXaC?Zy9!z_^J3vHru>79SgEb5_8*yFLDI5$O1fCHbT9HPrXbTZahn5A>@$5qa&u D_{`Xn From 71987b6d5a4cc20783490d9205eb6b42eae55552 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Fri, 7 May 2021 09:36:35 +0200 Subject: [PATCH 116/146] more flexible mirtrace protocol selection --- main.nf | 13 ++++++++++--- nextflow.config | 1 + nextflow_schema.json | 7 ++++++- 3 files changed, 17 insertions(+), 4 deletions(-) diff --git a/main.nf b/main.nf index a14e7ea5..dc8cc350 100644 --- a/main.nf +++ b/main.nf @@ -73,6 +73,13 @@ if (params.protocol == "illumina"){ protocol = params.protocol } +// mirtrace protocol defaults to 'params.protocol' if not set +if (!params.mirtrace_protocol){ + mirtrace_protocol = protocol +} else { + mirtrace_protocol = params.mirtrace_protocol +} + if (params.mirna_gtf) { mirna_gtf = file(params.mirna_gtf, checkIfExists: true) } else { @@ -162,6 +169,7 @@ summary['Reference Genome'] = params.fasta if(params.bt_index) summary['Bowtie Index for Ref'] = params.bt_index summary['Save Reference'] = params.save_reference ? 'Yes' : 'No' summary['Protocol'] = params.protocol +summary['Mirtrace Protocol'] = mirtrace_protocol summary['miRTrace species'] = params.mirtrace_species summary["3' adapter"] = three_prime_adapter summary['Output dir'] = params.outdir @@ -826,8 +834,7 @@ process mirtrace { file '*mirtrace' into mirtrace_results script: - primer = (protocol=="cats") ? " " : " --adapter $three_prime_adapter " - protocol_opt = (protocol=="custom") ? " " : " --protocol $protocol " + primer = (mirtrace_protocol=="cats") ? " " : " --adapter $three_prime_adapter " """ for i in $reads do @@ -839,7 +846,7 @@ process mirtrace { mirtrace qc \\ --species $params.mirtrace_species \\ $primer \\ - $protocol_opt \\ + --protocol $mirtrace_protocol \\ --config mirtrace_config \\ --write-fasta \\ --output-dir mirtrace \\ diff --git a/nextflow.config b/nextflow.config index 6b0bcf2d..9bc08a47 100644 --- a/nextflow.config +++ b/nextflow.config @@ -20,6 +20,7 @@ params { fasta = false bt_index = false mirtrace_species = false + mirtrace_protocol = false references_parsed = false mature = "ftp://mirbase.org/pub/mirbase/CURRENT/mature.fa.gz" hairpin = "ftp://mirbase.org/pub/mirbase/CURRENT/hairpin.fa.gz" diff --git a/nextflow_schema.json b/nextflow_schema.json index 704a4862..2c914a27 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -391,5 +391,10 @@ { "$ref": "#/definitions/institutional_config_options" } - ] + ], + "properties": { + "mirtrace_protocol": { + "type": "string" + } + } } \ No newline at end of file From d81dc8ae64ccc39c83948d3b4c1f874ccf596478 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Fri, 7 May 2021 09:43:04 +0200 Subject: [PATCH 117/146] added trim_galore_max_length option --- CHANGELOG.md | 2 ++ main.nf | 2 +- nextflow.config | 2 ++ 3 files changed, 5 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 28cd0450..90d97cce 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,6 +22,8 @@ * Allow `.fa` or `.fa.gz` files for mature and hairpin FASTA files. * Add full-size benchmark / test dataset to run on AWS for each release (see `test_full.config`) * Add `-f` flag to `gunzip` commands to deal with soft-links +* Add `--mirtrace_protocol` parameter to allow for more flexible selection of this parameter +* Added `--trim_galore_max_length` option [[#77](https://github.com/nf-core/smrnaseq/issues/77)] ### Packaged software updates diff --git a/main.nf b/main.nf index dc8cc350..164cc35d 100644 --- a/main.nf +++ b/main.nf @@ -383,7 +383,7 @@ process trim_galore { tpc_r1 = three_prime_clip_r1 > 0 ? "--three_prime_clip_r1 ${three_prime_clip_r1}" : '' tpa = (protocol == "qiaseq" | protocol == "cats") ? "--adapter ${three_prime_adapter}" : '--small_rna' """ - trim_galore --adapter ${three_prime_adapter} $tg_length $c_r1 $tpc_r1 --max_length 40 --gzip $reads --fastqc + trim_galore --adapter ${three_prime_adapter} $tg_length $c_r1 $tpc_r1 --max_length ${params.trim_galore_max_length} --gzip $reads --fastqc """ } diff --git a/nextflow.config b/nextflow.config index 9bc08a47..66fb1b6a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,6 +25,7 @@ params { mature = "ftp://mirbase.org/pub/mirbase/CURRENT/mature.fa.gz" hairpin = "ftp://mirbase.org/pub/mirbase/CURRENT/hairpin.fa.gz" + // Trimming options clip_r1 = 0 three_prime_clip_r1 = 0 three_prime_adapter = "TGGAATTCTCGGGTGCCAAGG" @@ -35,6 +36,7 @@ params { skip_mirdeep = false save_reference = true seq_center = "" + trim_galore_max_length = 40 // Boilerplate options multiqc_config = false From 617f52269e5aaf61e456d01d5c7b54f0828901cf Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Fri, 7 May 2021 10:11:25 +0200 Subject: [PATCH 118/146] solved memory usage issues --- CHANGELOG.md | 1 + main.nf | 5 ++++- nextflow_schema.json | 4 ++++ 3 files changed, 9 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 90d97cce..3c063044 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -24,6 +24,7 @@ * Add `-f` flag to `gunzip` commands to deal with soft-links * Add `--mirtrace_protocol` parameter to allow for more flexible selection of this parameter * Added `--trim_galore_max_length` option [[#77](https://github.com/nf-core/smrnaseq/issues/77)] +* Solved memory usage issue for mirtrace process [[#68](https://github.com/nf-core/smrnaseq/issues/68)] ### Packaged software updates diff --git a/main.nf b/main.nf index 164cc35d..08ddb4c3 100644 --- a/main.nf +++ b/main.nf @@ -824,6 +824,7 @@ process mirdeep2 { * STEP 8 - miRTrace */ process mirtrace { + label 'process_medium' tag "$reads" publishDir "${params.outdir}/miRTrace", mode: 'copy' @@ -835,7 +836,9 @@ process mirtrace { script: primer = (mirtrace_protocol=="cats") ? " " : " --adapter $three_prime_adapter " + memory = task.memory.toString().replaceAll("\\s", "").replaceAll("B", "") """ + export mirtracejar=\$(dirname \$(which mirtrace)) for i in $reads do path=\$(realpath \${i}) @@ -843,7 +846,7 @@ process mirtrace { echo \$path","\$prefix done > mirtrace_config - mirtrace qc \\ + java -Xms${memory} -Xmx${memory} -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace qc \\ --species $params.mirtrace_species \\ $primer \\ --protocol $mirtrace_protocol \\ diff --git a/nextflow_schema.json b/nextflow_schema.json index 2c914a27..40124325 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -395,6 +395,10 @@ "properties": { "mirtrace_protocol": { "type": "string" + }, + "trim_galore_max_length": { + "type": "integer", + "default": 40 } } } \ No newline at end of file From 061dc88c808600dd0c1b30f835940a9f8d4fb7ed Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Fri, 7 May 2021 10:17:15 +0200 Subject: [PATCH 119/146] updated schema --- nextflow_schema.json | 27 +++++++++++++++++---------- 1 file changed, 17 insertions(+), 10 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 40124325..40377844 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -153,6 +153,22 @@ "default": "TGGAATTCTCGGGTGCCAAGG", "fa_icon": "fas fa-text-width", "description": "Sequencing adapter sequence to search for and remove." + }, + "mirtrace_protocol": { + "type": "string", + "default": "illumina", + "description": "protocol to be used for the mirtrace process", + "enum": [ + "illumina", + "cats", + "qiaseq", + "nextflex" + ] + }, + "trim_galore_max_length": { + "type": "integer", + "default": 40, + "description": "trimgalore max-length parameter" } } }, @@ -391,14 +407,5 @@ { "$ref": "#/definitions/institutional_config_options" } - ], - "properties": { - "mirtrace_protocol": { - "type": "string" - }, - "trim_galore_max_length": { - "type": "integer", - "default": 40 - } - } + ] } \ No newline at end of file From 20c827941b7847168d6381558587025d4b829c89 Mon Sep 17 00:00:00 2001 From: Sarah Djebali Quelen Date: Fri, 7 May 2021 16:56:44 +0200 Subject: [PATCH 120/146] remove spaces in genome headers --- main.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index e8c601aa..bc4fc873 100644 --- a/main.nf +++ b/main.nf @@ -798,10 +798,11 @@ process mirdeep2 { script: """ perl -ane 's/[ybkmrsw]/N/ig;print;' $hairpin > hairpin_ok.fa - + sed 's/ .*//' $refgenome | awk '\$1 ~ /^>/ {gsub(/_/,"",\$1); print; next} {print}' > genome_nowhitespace.fa + miRDeep2.pl \\ $reads_collapsed \\ - $refgenome \\ + genome_nowhitespace.fa \\ $reads_vs_refdb \\ $mature \\ none \\ From 7b065ab54402ed85f37e5872688ccd887a37421b Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Mon, 10 May 2021 08:14:16 +0200 Subject: [PATCH 121/146] updated mirtrace in software versions --- main.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 08ddb4c3..9b6f3506 100644 --- a/main.nf +++ b/main.nf @@ -238,7 +238,9 @@ process get_software_versions { file "software_versions.csv" script: + memory = task.memory.toString().replaceAll("\\s", "").replaceAll("B", "") """ + export mirtracejar=\$(dirname \$(which mirtrace)) echo $workflow.manifest.version > v_pipeline.txt echo $workflow.nextflow.version > v_nextflow.txt echo \$(R --version 2>&1) > v_R.txt @@ -248,7 +250,7 @@ process get_software_versions { samtools --version > v_samtools.txt htseq-count -h > v_htseq.txt fasta_formatter -h > v_fastx.txt - mirtrace --version > v_mirtrace.txt + java -Xms${memory} -Xmx${memory} -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace --version > v_mirtrace.txt multiqc --version > v_multiqc.txt miRDeep2.pl -h > v_mirdeep2.txt From a62bd5def9dd671d9af62125e897d73ac0093773 Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Mon, 10 May 2021 09:38:31 +0200 Subject: [PATCH 122/146] Update main.nf Co-authored-by: Phil Ewels --- main.nf | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 9b6f3506..1afc931b 100644 --- a/main.nf +++ b/main.nf @@ -238,7 +238,11 @@ process get_software_versions { file "software_versions.csv" script: - memory = task.memory.toString().replaceAll("\\s", "").replaceAll("B", "") + java_mem = '' + if(task.memory){ + tmem = task.memory.toBytes() + java_mem = "-Xms${tmem} -Xmx${tmem}" + } """ export mirtracejar=\$(dirname \$(which mirtrace)) echo $workflow.manifest.version > v_pipeline.txt From af5718df860ea2f146059e4e20022886ec35f391 Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Mon, 10 May 2021 09:38:36 +0200 Subject: [PATCH 123/146] Update main.nf Co-authored-by: Phil Ewels --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 1afc931b..d2d34d3e 100644 --- a/main.nf +++ b/main.nf @@ -254,7 +254,7 @@ process get_software_versions { samtools --version > v_samtools.txt htseq-count -h > v_htseq.txt fasta_formatter -h > v_fastx.txt - java -Xms${memory} -Xmx${memory} -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace --version > v_mirtrace.txt + java $java_mem -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace --version > v_mirtrace.txt multiqc --version > v_multiqc.txt miRDeep2.pl -h > v_mirdeep2.txt From 937914378b06033f02afb30d495219a3594b5114 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Mon, 10 May 2021 09:41:52 +0200 Subject: [PATCH 124/146] update mirtrace process --- main.nf | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index d2d34d3e..33fef1c1 100644 --- a/main.nf +++ b/main.nf @@ -842,7 +842,11 @@ process mirtrace { script: primer = (mirtrace_protocol=="cats") ? " " : " --adapter $three_prime_adapter " - memory = task.memory.toString().replaceAll("\\s", "").replaceAll("B", "") + java_mem = '' + if(task.memory){ + tmem = task.memory.toBytes() + java_mem = "-Xms${tmem} -Xmx${tmem}" + } """ export mirtracejar=\$(dirname \$(which mirtrace)) for i in $reads @@ -852,7 +856,7 @@ process mirtrace { echo \$path","\$prefix done > mirtrace_config - java -Xms${memory} -Xmx${memory} -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace qc \\ + java $java_mem -jar \$mirtracejar/mirtrace.jar --mirtrace-wrapper-name mirtrace qc \\ --species $params.mirtrace_species \\ $primer \\ --protocol $mirtrace_protocol \\ From b3354e717c4f96a1c488cdf8ac1a872d9ceebb8a Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Mon, 10 May 2021 09:45:49 +0200 Subject: [PATCH 125/146] Update CHANGELOG.md Co-authored-by: Phil Ewels --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ee60af4f..fe903bbd 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,7 +2,7 @@ ## v1.1dev - [date] -* remove spaces in genome headers and replace special nt by N in hairpin file for mirdeep2 to work. Follow up from [[#69]] (https://github.com/nf-core/smrnaseq/pull/79) +* remove spaces in genome headers and replace special nt by N in hairpin file for mirdeep2 to work. [[#69]] (https://github.com/nf-core/smrnaseq/pull/79) * Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) * Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) * Change `--reads` to `--input` for consistency with rest of nf-core From d632153e39f4573f5ee38218ba51468daa57940b Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Mon, 10 May 2021 09:47:22 +0200 Subject: [PATCH 126/146] Update CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index fe903bbd..f5f68cf4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,7 +2,7 @@ ## v1.1dev - [date] -* remove spaces in genome headers and replace special nt by N in hairpin file for mirdeep2 to work. [[#69]] (https://github.com/nf-core/smrnaseq/pull/79) +* remove spaces in genome headers and replace special nt by N in hairpin file for mirdeep2 to work. [[#69]](https://github.com/nf-core/smrnaseq/pull/79) * Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) * Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) * Change `--reads` to `--input` for consistency with rest of nf-core From 18ebbdca9f659f89bd6bbeb3e826408705abe607 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 11 May 2021 07:06:09 +0200 Subject: [PATCH 127/146] updated changelog again --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 3c063044..d44fe65d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -24,7 +24,7 @@ * Add `-f` flag to `gunzip` commands to deal with soft-links * Add `--mirtrace_protocol` parameter to allow for more flexible selection of this parameter * Added `--trim_galore_max_length` option [[#77](https://github.com/nf-core/smrnaseq/issues/77)] -* Solved memory usage issue for mirtrace process [[#68](https://github.com/nf-core/smrnaseq/issues/68)] +* Solved memory usage issue for mirtrace in the main process and in the `get_software_versions` process [[#68](https://github.com/nf-core/smrnaseq/issues/68)] ### Packaged software updates From 079db0449092e96a846307eacb22d086c77dcc91 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 11 May 2021 08:07:37 +0200 Subject: [PATCH 128/146] bump version --- .github/workflows/awsfulltest.yml | 3 --- .github/workflows/awstest.yml | 3 --- .github/workflows/ci.yml | 4 ++-- Dockerfile | 4 ++-- environment.yml | 2 +- nextflow.config | 4 ++-- 6 files changed, 7 insertions(+), 13 deletions(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 18f84c8b..9634a41b 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -9,7 +9,6 @@ on: types: [completed] workflow_dispatch: - env: AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} @@ -18,7 +17,6 @@ env: AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} - jobs: run-awstest: name: Run AWS full tests @@ -33,7 +31,6 @@ jobs: - name: Install awscli run: conda install -c conda-forge awscli - name: Start AWS batch job - # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters # Then specify `-profile test_full` instead of `-profile test` on the AWS batch command diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 205e94fc..be4d04d3 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -6,7 +6,6 @@ name: nf-core AWS test on: workflow_dispatch: - env: AWS_ACCESS_KEY_ID: ${{ secrets.AWS_ACCESS_KEY_ID }} AWS_SECRET_ACCESS_KEY: ${{ secrets.AWS_SECRET_ACCESS_KEY }} @@ -15,7 +14,6 @@ env: AWS_JOB_QUEUE: ${{ secrets.AWS_JOB_QUEUE }} AWS_S3_BUCKET: ${{ secrets.AWS_S3_BUCKET }} - jobs: run-awstest: name: Run AWS tests @@ -30,7 +28,6 @@ jobs: - name: Install awscli run: conda install -c conda-forge awscli - name: Start AWS batch job - # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix run: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index d179cbc0..e4ab7f36 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -34,13 +34,13 @@ jobs: - name: Build new docker image if: env.MATCHED_FILES - run: docker build --no-cache . -t nfcore/smrnaseq:dev + run: docker build --no-cache . -t nfcore/smrnaseq:1.1.0 - name: Pull docker image if: ${{ !env.MATCHED_FILES }} run: | docker pull nfcore/smrnaseq:dev - docker tag nfcore/smrnaseq:dev nfcore/smrnaseq:dev + docker tag nfcore/smrnaseq:dev nfcore/smrnaseq:1.1.0 - name: Install Nextflow env: diff --git a/Dockerfile b/Dockerfile index e0c4ed6e..cc5dda91 100644 --- a/Dockerfile +++ b/Dockerfile @@ -10,7 +10,7 @@ COPY environment.yml / RUN conda env create --quiet -f /environment.yml && conda clean -a # Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1dev/bin:$PATH +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1.0/bin:$PATH # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-smrnaseq-1.1dev > nf-core-smrnaseq-1.1dev.yml +RUN conda env export --name nf-core-smrnaseq-1.1.0 > nf-core-smrnaseq-1.1.0.yml diff --git a/environment.yml b/environment.yml index 8465d6ba..85725d75 100644 --- a/environment.yml +++ b/environment.yml @@ -1,6 +1,6 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.1dev +name: nf-core-smrnaseq-1.1.0 channels: - conda-forge - bioconda diff --git a/nextflow.config b/nextflow.config index 66fb1b6a..5e88f8bc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -70,7 +70,7 @@ params { // Container slug. Stable releases should specify release tag! // Developmental code should specify :dev -process.container = 'nfcore/smrnaseq:dev' +process.container = 'nfcore/smrnaseq:1.1.0' // Load base.config by default for all pipelines includeConfig 'conf/base.config' @@ -176,7 +176,7 @@ manifest { description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' nextflowVersion = '>=20.04.0' - version = '1.1dev' + version = '1.1.0' } // Function to ensure that resource requirements don't go beyond From b450fa4dbca8b341018375f77cb5d61ce4dd9ada Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 11 May 2021 08:54:15 +0200 Subject: [PATCH 129/146] updated version in changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d44fe65d..6b640bb6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,6 +1,6 @@ # nf-core/smrnaseq: Changelog -## v1.1dev - [date] +## [v1.1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.1.0) - 2021-05-11 * Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) * Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) From 7f38820f1b4975e1afdb6dabd289a25b94258627 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 11 May 2021 09:27:41 +0200 Subject: [PATCH 130/146] better changelog description --- CHANGELOG.md | 14 ++++++++++++-- 1 file changed, 12 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 6b640bb6..b7dda0d6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -2,9 +2,20 @@ ## [v1.1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.1.0) - 2021-05-11 +### Major changes + +**:warning: Breaking changes!** + +This release contains several major (potentially breaking) changes: + +* The main input parameter has been changed from `--reads` to `--input` to standardize the pipeline with other nf-core pipelines +* All parameter documentation has moved into a new `nextflow_schema.json` file +* A `lib` folder with groovy helper classes has been added to the pipeline. This includes validation of input parameters using the schema defined in the `nextflow_schema.json` file + +### General improvements + * Accept custom genome and remove non-canonical letters in the genome. Thanks to @sdjebali. Follow up from [[#63]](https://github.com/nf-core/smrnaseq/pull/63) * Fix error when only one sample is in the input [[#31]](https://github.com/nf-core/smrnaseq/issues/31) -* Change `--reads` to `--input` for consistency with rest of nf-core * Made `CamelCase` pipeline parameters `snake_case` and lower case * `clip_R1` -> `clip_r1` * `three_prime_clip_R1` -> `three_prime_clip_r1` @@ -12,7 +23,6 @@ * `skipQC` -> `skip_qc` * `skipFastqc` -> `skip_fastqc` * `skipMultiqc` -> `skip_multiqc` -* Move all parameter documentation into new `nextflow_schema.json` file. * Update with the latest `TEMPLATE` version for nf-core `1.12.1` * Update conda environment with new packages and updates * Added `--protocol custom` to allow custom adapter trimming modes [[#41]](https://github.com/nf-core/smrnaseq/issues/41)] From 2f2e9c309cb4f2953781aeb87ac6f43a50b70fd1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 11 May 2021 11:09:56 +0000 Subject: [PATCH 131/146] Template update for nf-core/tools version 1.14 --- .github/PULL_REQUEST_TEMPLATE.md | 7 +++-- .github/workflows/ci.yml | 5 ++- CHANGELOG.md | 2 +- Dockerfile | 6 ++-- README.md | 2 +- environment.yml | 2 +- lib/NfcoreSchema.groovy | 54 +++++++++++++++++--------------- main.nf | 4 +-- nextflow.config | 18 ++++++----- nextflow_schema.json | 6 ++-- 10 files changed, 57 insertions(+), 49 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index edd0ac48..e74762ec 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,14 +10,15 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) --> + ## PR checklist - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) - - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. + - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` + - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](nf-core/smrnaseq/tree/master/.github/CONTRIBUTING.md) + - [ ] If necessary, also make a PR on the nf-core/smrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 42dbf66f..9b731512 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -8,6 +8,9 @@ on: release: types: [published] +# Uncomment if we need an edge release of Nextflow again +# env: NXF_EDGE: 1 + jobs: test: name: Run workflow tests @@ -20,7 +23,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', '21.03.0-edge'] + nxf_ver: ['20.04.0', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/CHANGELOG.md b/CHANGELOG.md index 9f967687..9b142a97 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.1dev - [date] +## v1.1.0 - [date] Initial release of nf-core/smrnaseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/Dockerfile b/Dockerfile index 46fe4be6..2a232cb4 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM nfcore/base:1.13.3 +FROM nfcore/base:1.14 LABEL authors="Phil Ewels , Chuan Wang , Rickard Hammarén , Lorena Pantano " \ description="Docker image containing all software requirements for the nf-core/smrnaseq pipeline" @@ -7,7 +7,7 @@ COPY environment.yml / RUN conda env create --quiet -f /environment.yml && conda clean -a # Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1dev/bin:$PATH +ENV PATH /opt/conda/envs/nf-core-smrnaseq-1.1.0/bin:$PATH # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-smrnaseq-1.1dev > nf-core-smrnaseq-1.1dev.yml +RUN conda env export --name nf-core-smrnaseq-1.1.0 > nf-core-smrnaseq-1.1.0.yml diff --git a/README.md b/README.md index 70516c0d..8b12c974 100644 --- a/README.md +++ b/README.md @@ -19,7 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ## Quick Start -1. Install [`nextflow`](https://nf-co.re/usage/installation) +1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ diff --git a/environment.yml b/environment.yml index 996cc1b0..942555a6 100644 --- a/environment.yml +++ b/environment.yml @@ -1,6 +1,6 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-smrnaseq-1.1dev +name: nf-core-smrnaseq-1.1.0 channels: - conda-forge - bioconda diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 54935ec8..52ee7304 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -112,8 +112,14 @@ class NfcoreSchema { } // unexpected params def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) { - unexpectedParams.push(specifiedParam) + def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } + def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + // Temporarily remove camelCase/camel-case params #1035 + def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} + if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ + unexpectedParams.push(specifiedParam) + } } } @@ -191,11 +197,11 @@ class NfcoreSchema { // Remove an element from a JSONArray private static JSONArray removeElement(jsonArray, element){ - def list = [] + def list = [] int len = jsonArray.length() - for (int i=0;i hnames.each { hname -> if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error '====================================================\n' + + log.error "${c_red}====================================================${c_reset}\n" + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - '============================================================' + "${c_red}====================================================${c_reset}\n" } } } diff --git a/nextflow.config b/nextflow.config index 80a90c08..c255e96f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,12 +25,13 @@ params { plaintext_email = false monochrome_logs = false help = false - igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_base = 's3://ngi-igenomes/igenomes' tracedir = "${params.outdir}/pipeline_info" igenomes_ignore = false custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" hostnames = false + config_profile_name = null config_profile_description = false config_profile_contact = false config_profile_url = false @@ -65,7 +66,7 @@ profiles { singularity.enabled = false podman.enabled = false shifter.enabled = false - charliecloud = false + charliecloud.enabled = false process.conda = "$projectDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } @@ -94,7 +95,7 @@ profiles { docker.enabled = false podman.enabled = true shifter.enabled = false - charliecloud = false + charliecloud.enabled = false } shifter { singularity.enabled = false @@ -129,21 +130,22 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true - file = "${params.tracedir}/execution_timeline.html" + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" } report { enabled = true - file = "${params.tracedir}/execution_report.html" + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" } trace { enabled = true - file = "${params.tracedir}/execution_trace.txt" + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" } dag { enabled = true - file = "${params.tracedir}/pipeline_dag.svg" + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" } manifest { @@ -153,7 +155,7 @@ manifest { description = 'Small RNA-Seq Best Practice Analysis Pipeline.' mainScript = 'main.nf' nextflowVersion = '>=20.04.0' - version = '1.1dev' + version = '1.1.0' } // Function to ensure that resource requirements don't go beyond diff --git a/nextflow_schema.json b/nextflow_schema.json index b9e417f6..a8691067 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -62,7 +62,7 @@ "igenomes_base": { "type": "string", "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes/", + "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", "hidden": true }, @@ -183,7 +183,7 @@ "description": "Maximum amount of memory that can be requested for any single job.", "default": "128.GB", "fa_icon": "fas fa-memory", - "pattern": "^[\\d\\.]+\\s*.(K|M|G|T)?B$", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", "hidden": true, "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" }, @@ -192,7 +192,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", - "pattern": "^[\\d\\.]+\\.*(s|m|h|d)$", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } From 4c67307873a63b8e387f31f44b1b93d4ee729ee9 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Wed, 12 May 2021 09:16:41 +0200 Subject: [PATCH 132/146] cleaned up parameters --- main.nf | 1 - nextflow.config | 10 ++++++---- nextflow_schema.json | 9 +++++++++ 3 files changed, 15 insertions(+), 5 deletions(-) diff --git a/main.nf b/main.nf index 4e70fca3..f5ef2b1c 100644 --- a/main.nf +++ b/main.nf @@ -181,7 +181,6 @@ summary['Current path'] = "$PWD" summary['Script dir'] = workflow.projectDir summary['Config Profile'] = (workflow.profile == 'standard' ? 'UPPMAX' : workflow.profile) summary['Fasta Ref'] = params.fasta -summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" summary['Output dir'] = params.outdir diff --git a/nextflow.config b/nextflow.config index d6374719..8f4ea1e0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -15,10 +15,12 @@ params { protocol = 'illumina' // Reference genomes - genome = false - mirna_gtf = false - fasta = false - bt_index = false + genome = null + gtf = null + mirna_gtf = null + fasta = null + bt_index = null + bt_indices = null mirtrace_species = false mirtrace_protocol = false references_parsed = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 83b56756..1660a5a8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -122,6 +122,15 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "gtf": { + "type": "string", + "description": "Path to reference genome GTF file", + "fa_icon": "fas fa-address-book" + }, + "bt_indices": { + "type": "array", + "default": "null" } } }, From f51bb96a0f81e24746d23d76ad887a1fb1527a24 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Wed, 12 May 2021 09:20:02 +0200 Subject: [PATCH 133/146] changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 4b1090f3..9e610362 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -36,6 +36,7 @@ This release contains several major (potentially breaking) changes: * Add `--mirtrace_protocol` parameter to allow for more flexible selection of this parameter * Added `--trim_galore_max_length` option [[#77](https://github.com/nf-core/smrnaseq/issues/77)] * Solved memory usage issue for mirtrace in the main process and in the `get_software_versions` process [[#68](https://github.com/nf-core/smrnaseq/issues/68)] +* Removed logging of `single_end` parameter and added missing parameters to schema and config files ### Packaged software updates From b308737ee237e35ecf1df3a8feeec8eaec8ef9dd Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Wed, 12 May 2021 09:36:40 +0200 Subject: [PATCH 134/146] fixed bug; updated some versions --- environment.yml | 16 ++++++++-------- nextflow_schema.json | 2 +- 2 files changed, 9 insertions(+), 9 deletions(-) diff --git a/environment.yml b/environment.yml index 85725d75..09b1e26d 100644 --- a/environment.yml +++ b/environment.yml @@ -15,20 +15,20 @@ dependencies: - conda-forge::python=3.7.3 - conda-forge::markdown=3.1.1 - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.6.1 - - conda-forge::pigz=2.3.4 + - conda-forge::pygments=2.9.0 + - conda-forge::pigz=2.6 # bioconda packages - bioconda::fastqc=0.11.9 - - bioconda::trim-galore=0.6.5 - - bioconda::samtools=1.9 - - bioconda::bowtie=1.2.3 - - bioconda::multiqc=1.9 + - bioconda::trim-galore=0.6.6 + - bioconda::samtools=1.12 + - bioconda::bowtie=1.3.0 + - bioconda::multiqc=1.10.1 - bioconda::mirtop=0.4.23 - bioconda::seqcluster=1.2.7 - - bioconda::htseq=0.11.3 + - bioconda::htseq=0.13.5 - bioconda::fastx_toolkit=0.0.14 - - bioconda::seqkit=0.12.0 + - bioconda::seqkit=0.16.0 - bioconda::mirtrace=1.0.1 - bioconda::bioconductor-edger=3.26.5 - bioconda::bioconductor-limma=3.40.2 diff --git a/nextflow_schema.json b/nextflow_schema.json index 1660a5a8..7f31fbfa 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -130,7 +130,7 @@ }, "bt_indices": { "type": "array", - "default": "null" + "description": "List of bowtie index files" } } }, From f6e843c6d2468125699a6fa3fb793519f593f102 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Wed, 12 May 2021 12:56:33 +0200 Subject: [PATCH 135/146] added custom into protocol enum --- CHANGELOG.md | 1 + nextflow_schema.json | 3 ++- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9e610362..8ec3f785 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -37,6 +37,7 @@ This release contains several major (potentially breaking) changes: * Added `--trim_galore_max_length` option [[#77](https://github.com/nf-core/smrnaseq/issues/77)] * Solved memory usage issue for mirtrace in the main process and in the `get_software_versions` process [[#68](https://github.com/nf-core/smrnaseq/issues/68)] * Removed logging of `single_end` parameter and added missing parameters to schema and config files +* Added "custom" as option for `--protocol` in the `nextflow_schema.json` ### Packaged software updates diff --git a/nextflow_schema.json b/nextflow_schema.json index 7f31fbfa..307f8dd1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -30,7 +30,8 @@ "illumina", "nextflex", "qiaseq", - "cats" + "cats", + "custom" ] }, "outdir": { From 215835c868e36384dcfa5f1d3402a68e5d64c991 Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Mon, 17 May 2021 07:09:22 +0200 Subject: [PATCH 136/146] Update .github/workflows/awsfulltest.yml Co-authored-by: Gisela Gabernet --- .github/workflows/awsfulltest.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9634a41b..ae64258f 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -40,4 +40,4 @@ jobs: --job-name nf-core-smrnaseq \ --job-queue $AWS_JOB_QUEUE \ --job-definition $AWS_JOB_DEFINITION \ - --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' + --container-overrides '{"command": ["nf-core/smrnaseq", "-r '"${GITHUB_SHA}"' -profile test_full --outdir s3://'"${AWS_S3_BUCKET}"'/smrnaseq/results-'"${GITHUB_SHA}"' -w s3://'"${AWS_S3_BUCKET}"'/smrnaseq/work-'"${GITHUB_SHA}"' -with-tower"], "environment": [{"name": "TOWER_ACCESS_TOKEN", "value": "'"$TOWER_ACCESS_TOKEN"'"}]}' From 968c7b06d1724b0d34ac3a74e6f0c4b569d1aebc Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Mon, 17 May 2021 12:29:09 +0200 Subject: [PATCH 137/146] removed genome parameters --- nextflow.config | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/nextflow.config b/nextflow.config index 8f4ea1e0..531d5f56 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,11 +17,7 @@ params { // Reference genomes genome = null gtf = null - mirna_gtf = null - fasta = null - bt_index = null bt_indices = null - mirtrace_species = false mirtrace_protocol = false references_parsed = false mature = "ftp://mirbase.org/pub/mirbase/CURRENT/mature.fa.gz" @@ -48,7 +44,7 @@ params { plaintext_email = false monochrome_logs = false help = false - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' tracedir = "${params.outdir}/pipeline_info" igenomes_ignore = false custom_config_version = 'master' From 93ef876db04fb4a37bac2e4eeae19c67bcdd5efb Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Mon, 17 May 2021 13:36:39 +0200 Subject: [PATCH 138/146] adjusted bowtie_indices projcess --- main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/main.nf b/main.nf index f5ef2b1c..d7db2b92 100644 --- a/main.nf +++ b/main.nf @@ -276,7 +276,7 @@ if (!params.references_parsed && !params.skip_mirdeep){ file hairpin from reference_hairpin output: - file 'genome.fa' into fasta + file 'genome.edited.fa' into fasta file 'genome.*.ebwt' into indices_mirdeep2 file 'hairpin.fa' into hairpin file 'mature.fa' into mature @@ -296,14 +296,14 @@ if (!params.references_parsed && !params.skip_mirdeep){ fi # Remove any special base characters from reference genome FASTA file - sed '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome > genome.fa + sed '/^[^>]/s/[^ATGCatgc]/N/g' $refgenome > genome.edited.fa # Remove spaces from miRBase FASTA files sed -i 's, ,_,g' \$HAIRPIN sed -i 's, ,_,g' \$MATURE # Build bowtie index - bowtie-build genome.fa genome --threads ${task.cpus} + bowtie-build genome.edited.fa genome --threads ${task.cpus} """ } } From eea3dd2e3cd6c2df6208077913c3bcb7f529d029 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Wed, 19 May 2021 09:17:28 +0200 Subject: [PATCH 139/146] use bug-fixed NfcoreSchema.groovy file --- .nf-core-lint.yml | 2 ++ lib/NfcoreSchema.groovy | 5 +++-- 2 files changed, 5 insertions(+), 2 deletions(-) create mode 100644 .nf-core-lint.yml diff --git a/.nf-core-lint.yml b/.nf-core-lint.yml new file mode 100644 index 00000000..27e3032a --- /dev/null +++ b/.nf-core-lint.yml @@ -0,0 +1,2 @@ +files_unchanged: + - lib/NfcoreSchema.groovy diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 52ee7304..ac7f49e8 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -114,7 +114,8 @@ class NfcoreSchema { def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { // Temporarily remove camelCase/camel-case params #1035 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ @@ -570,4 +571,4 @@ class NfcoreSchema { return output } -} +} \ No newline at end of file From ad2d3de14a516300f6032397a0f5177f429ac361 Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Wed, 19 May 2021 11:19:37 +0200 Subject: [PATCH 140/146] Update nextflow.config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 531d5f56..70cbbf6b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -44,7 +44,7 @@ params { plaintext_email = false monochrome_logs = false help = false - igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_base = 's3://ngi-igenomes/igenomes' tracedir = "${params.outdir}/pipeline_info" igenomes_ignore = false custom_config_version = 'master' From 3735334f0226903a343557b1e1b3f8b66244cf4f Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 25 May 2021 10:09:17 +0200 Subject: [PATCH 141/146] fixed nfcoreschema bug --- lib/NfcoreSchema.groovy | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 52ee7304..9e77a71d 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -114,7 +114,8 @@ class NfcoreSchema { def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { // Temporarily remove camelCase/camel-case params #1035 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ From 810f5ed22dd1a26765eeac5349da502eb39c6672 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 25 May 2021 10:20:13 +0200 Subject: [PATCH 142/146] fixed edgeR mirbase bug (1 sample) --- bin/edgeR_miRBase.r | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/bin/edgeR_miRBase.r b/bin/edgeR_miRBase.r index 388e4ef0..dce016a3 100755 --- a/bin/edgeR_miRBase.r +++ b/bin/edgeR_miRBase.r @@ -92,7 +92,7 @@ for (i in 1:2) { dataNorm_df<-as.data.frame(cpm(dataNorm)) write.table(dataNorm_df,file=paste(header,"_normalized_CPM.txt",sep=""),sep='\t',quote=FALSE) - if (ncol(data)>1){ # with more than 1 sample + if (length(filelist[[1]]) > 1){ # with more than 1 sample # Print heatmap based on normalized read counts pdf(paste(header,"_CPM_heatmap.pdf",sep="")) heatmap.2(cpm(dataNorm),col=redgreen(100),key=TRUE,scale="row",density.info="none",trace="none") @@ -100,7 +100,7 @@ for (i in 1:2) { } # Make MDS plot (only perform with 3 or more samples) - if (ncol(data)>2){ + if (length(filelist[[1]]) > 2){ pdf(paste(header,"_edgeR_MDS_plot.pdf",sep="")) MDSdata <- plotMDS(dataNorm) dev.off() From 3d52a6b7f539d8cee04c256510285a5a16057759 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Tue, 25 May 2021 14:49:25 +0200 Subject: [PATCH 143/146] additional check in edgeR script --- bin/edgeR_miRBase.r | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/bin/edgeR_miRBase.r b/bin/edgeR_miRBase.r index dce016a3..b98cc9e5 100755 --- a/bin/edgeR_miRBase.r +++ b/bin/edgeR_miRBase.r @@ -68,8 +68,13 @@ for (i in 1:2) { # Remove genes with 0 reads in all samples row_sub = apply(data, 1, function(row) all(row ==0 )) - - data<-data[!row_sub,] + # Only subset if at least one sample is remaining + nr_keep <- table(row_sub) + nr_keep <- as.numeric(nr_keep[names(nr_keep) == TRUE]) + if (nr_keep > 0){ + data<-data[!row_sub,] + } + write.csv(t(data),file=paste(header,"_counts.csv",sep="")) # Normalization From 06413d00274591bd409367daf8f232ab5f2884c8 Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Thu, 10 Jun 2021 14:09:44 +0200 Subject: [PATCH 144/146] replace gtf with mirna_gtf --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 70cbbf6b..f97fb3e8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,7 +16,7 @@ params { // Reference genomes genome = null - gtf = null + mirna_gtf = null bt_indices = null mirtrace_protocol = false references_parsed = false From 179df50f81bf69d18a66f3d7939d47d138109c5c Mon Sep 17 00:00:00 2001 From: kevinmenden Date: Thu, 10 Jun 2021 14:11:25 +0200 Subject: [PATCH 145/146] remove gtf from schema --- nextflow_schema.json | 5 ----- 1 file changed, 5 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 307f8dd1..3c9c6e21 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -124,11 +124,6 @@ "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." }, - "gtf": { - "type": "string", - "description": "Path to reference genome GTF file", - "fa_icon": "fas fa-address-book" - }, "bt_indices": { "type": "array", "description": "List of bowtie index files" From b3f3e4df7ea5d32afee7c6ba8f88e2df08086edb Mon Sep 17 00:00:00 2001 From: Kevin Menden Date: Tue, 15 Jun 2021 14:10:28 +0200 Subject: [PATCH 146/146] update release date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8ec3f785..d4a4c3c0 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,6 +1,6 @@ # nf-core/smrnaseq: Changelog -## [v1.1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.1.0) - 2021-05-11 +## [v1.1.0](https://github.com/nf-core/smrnaseq/releases/tag/1.1.0) - 2021-06-15 ### Major changes

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z9DXaC?Zy9!z_^J3vHru>79SgEb5_8*yFLDI5$O1fCHbT9HPrXbTZahn5A>@$5qa&u D_{`Xn diff --git a/docs/output.md b/docs/output.md index 9232e4cf..d061904c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,9 +1,5 @@ # nf-core/smrnaseq: Output -## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/smrnaseq/output](https://nf-co.re/smrnaseq/output) - -> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ - ## Introduction This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. diff --git a/docs/usage.md b/docs/usage.md index c5e9d84d..361ce6b9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -51,7 +51,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -71,8 +71,14 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof * `podman` * A generic configuration profile to be used with [Podman](https://podman.io/) * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) +* `shifter` + * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) + * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) +* `charliecloud` + * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) + * Pulls software from Docker Hub: [`nfcore/smrnaseq`](https://hub.docker.com/r/nfcore/smrnaseq/) * `conda` - * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity or Podman. + * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. * A generic configuration profile to be used with [Conda](https://conda.io/docs/) * Pulls most software from [Bioconda](https://bioconda.github.io/) * `test` @@ -103,6 +109,8 @@ process { } ``` +To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`. + See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. diff --git a/lib/Headers.groovy b/lib/Headers.groovy new file mode 100644 index 00000000..15d1d388 --- /dev/null +++ b/lib/Headers.groovy @@ -0,0 +1,43 @@ +/* + * This file holds several functions used to render the nf-core ANSI header. + */ + +class Headers { + + private static Map log_colours(Boolean monochrome_logs) { + Map colorcodes = [:] + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" + return colorcodes + } + + static String dashed_line(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + static String nf_core(workflow, monochrome_logs) { + Map colors = log_colours(monochrome_logs) + String.format( + """\n + ${dashed_line(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashed_line(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy new file mode 100644 index 00000000..78e8c65d --- /dev/null +++ b/lib/NfcoreSchema.groovy @@ -0,0 +1,571 @@ +/* + * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. + */ + +import org.everit.json.schema.Schema +import org.everit.json.schema.loader.SchemaLoader +import org.everit.json.schema.ValidationException +import org.json.JSONObject +import org.json.JSONTokener +import org.json.JSONArray +import groovy.json.JsonSlurper +import groovy.json.JsonBuilder + +class NfcoreSchema { + + /* + * Function to loop over all parameters defined in schema and check + * whether the given paremeters adhere to the specificiations + */ + /* groovylint-disable-next-line UnusedPrivateMethodParameter */ + private static void validateParameters(params, jsonSchema, log) { + def has_error = false + //=====================================================================// + // Check for nextflow core params and unexpected params + def json = new File(jsonSchema).text + def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') + def nf_params = [ + // Options for base `nextflow` command + 'bg', + 'c', + 'C', + 'config', + 'd', + 'D', + 'dockerize', + 'h', + 'log', + 'q', + 'quiet', + 'syslog', + 'v', + 'version', + + // Options for `nextflow run` command + 'ansi', + 'ansi-log', + 'bg', + 'bucket-dir', + 'c', + 'cache', + 'config', + 'dsl2', + 'dump-channels', + 'dump-hashes', + 'E', + 'entry', + 'latest', + 'lib', + 'main-script', + 'N', + 'name', + 'offline', + 'params-file', + 'pi', + 'plugins', + 'poll-interval', + 'pool-size', + 'profile', + 'ps', + 'qs', + 'queue-size', + 'r', + 'resume', + 'revision', + 'stdin', + 'stub', + 'stub-run', + 'test', + 'w', + 'with-charliecloud', + 'with-conda', + 'with-dag', + 'with-docker', + 'with-mpi', + 'with-notification', + 'with-podman', + 'with-report', + 'with-singularity', + 'with-timeline', + 'with-tower', + 'with-trace', + 'with-weblog', + 'without-docker', + 'without-podman', + 'work-dir' + ] + def unexpectedParams = [] + + // Collect expected parameters from the schema + def expectedParams = [] + for (group in schemaParams) { + for (p in group.value['properties']) { + expectedParams.push(p.key) + } + } + + for (specifiedParam in params.keySet()) { + // nextflow params + if (nf_params.contains(specifiedParam)) { + log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" + has_error = true + } + // unexpected params + def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam)) { + unexpectedParams.push(specifiedParam) + } + } + + //=====================================================================// + // Validate parameters against the schema + InputStream inputStream = new File(jsonSchema).newInputStream() + JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream)) + + // Remove anything that's in params.schema_ignore_params + rawSchema = removeIgnoredParams(rawSchema, params) + + Schema schema = SchemaLoader.load(rawSchema) + + // Clean the parameters + def cleanedParams = cleanParameters(params) + + // Convert to JSONObject + def jsonParams = new JsonBuilder(cleanedParams) + JSONObject paramsJSON = new JSONObject(jsonParams.toString()) + + // Validate + try { + schema.validate(paramsJSON) + } catch (ValidationException e) { + println '' + log.error 'ERROR: Validation of pipeline parameters failed!' + JSONObject exceptionJSON = e.toJSON() + printExceptions(exceptionJSON, paramsJSON, log) + println '' + has_error = true + } + + // Check for unexpected parameters + if (unexpectedParams.size() > 0) { + Map colors = log_colours(params.monochrome_logs) + println '' + def warn_msg = 'Found unexpected parameters:' + for (unexpectedParam in unexpectedParams) { + warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" + } + log.warn warn_msg + log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" + println '' + } + + if (has_error) { + System.exit(1) + } + } + + // Loop over nested exceptions and print the causingException + private static void printExceptions(exJSON, paramsJSON, log) { + def causingExceptions = exJSON['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (exJSON['pointerToViolation'] == '#') { + log.error "* ${exJSON['message']}" + } + // Error with specific param + else { + def param = exJSON['pointerToViolation'] - ~/^#\// + def param_val = paramsJSON[param].toString() + log.error "* --${param}: ${exJSON['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, paramsJSON, log) + } + } + + // Remove an element from a JSONArray + private static JSONArray removeElement(jsonArray, element){ + def list = [] + int len = jsonArray.length() + for (int i=0;i + if(rawSchema.keySet().contains('definitions')){ + rawSchema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').containsKey(ignore_param)) { + rawSchema.get("properties").remove(ignore_param) + } + if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { + def cleaned_required = removeElement(rawSchema.required, ignore_param) + rawSchema.put("required", cleaned_required) + } + } + return rawSchema + } + + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + /* + * This method tries to read a JSON params file + */ + private static LinkedHashMap params_load(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = params_read(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + private static Map log_colours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + static String dashed_line(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + /* + Method to actually read in JSON file using Groovy. + Group (as Key), values are all parameters + - Parameter1 as Key, Description as Value + - Parameter2 as Key, Description as Value + .... + Group + - + */ + private static LinkedHashMap params_read(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + /* + * Get maximum number of characters across all parameter names + */ + private static Integer params_max_chars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } + + /* + * Beautify parameters for --help + */ + private static String params_help(workflow, params, json_schema, command) { + Map colors = log_colours(params.monochrome_logs) + Integer num_hidden = 0 + String output = '' + output += 'Typical pipeline command:\n\n' + output += " ${colors.cyan}${command}${colors.reset}\n\n" + Map params_map = params_load(json_schema) + Integer max_chars = params_max_chars(params_map) + 1 + Integer desc_indent = max_chars + 14 + Integer dec_linewidth = 160 - desc_indent + for (group in params_map.keySet()) { + Integer num_params = 0 + String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (group_params.get(param).hidden && !params.show_hidden_params) { + num_hidden += 1 + continue; + } + def type = '[' + group_params.get(param).type + ']' + def description = group_params.get(param).description + def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : '' + def description_default = description + colors.dim + defaultValue + colors.reset + // Wrap long description texts + // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap + if (description_default.length() > dec_linewidth){ + List olines = [] + String oline = "" // " " * indent + description_default.split(" ").each() { wrd -> + if ((oline.size() + wrd.size()) <= dec_linewidth) { + oline += wrd + " " + } else { + olines += oline + oline = wrd + " " + } + } + olines += oline + description_default = olines.join("\n" + " " * desc_indent) + } + group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' + num_params += 1 + } + group_output += '\n' + if (num_params > 0){ + output += group_output + } + } + output += dashed_line(params.monochrome_logs) + if (num_hidden > 0){ + output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset + output += dashed_line(params.monochrome_logs) + } + return output + } + + /* + * Groovy Map summarising parameters/workflow options used by the pipeline + */ + private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = "$workflow.containerEngine" + } + if (workflow.container) { + workflow_summary['container'] = "$workflow.container" + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def blacklist = ['hostnames'] + def params_map = params_load(json_schema) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param) && !blacklist.contains(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value == null) { + if (param_type == 'boolean') { + schema_value = false + } + if (param_type == 'string') { + schema_value = '' + } + if (param_type == 'integer') { + schema_value = 0 + } + } else { + if (param_type == 'string') { + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir', '') + sub_string = sub_string.replace('\${projectDir}', '') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir', '') + sub_string = sub_string.replace('\${params.outdir}', '') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } + + if (params_value != schema_value) { + sub_params.put("$param", params_value) + } + } + } + params_summary.put(group, sub_params) + } + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + /* + * Beautify parameters for summary and return as string + */ + private static String params_summary_log(workflow, params, json_schema) { + String output = '' + def params_map = params_summary_map(workflow, params, json_schema) + def max_chars = params_max_chars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += group + '\n' + for (param in group_params.keySet()) { + output += " \u001B[1m" + param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + '\n' + } + output += '\n' + } + } + output += "[Only displaying parameters that differ from pipeline default]\n" + output += dashed_line(params.monochrome_logs) + output += '\n\n' + dashed_line(params.monochrome_logs) + return output + } + +} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar new file mode 100644 index 0000000000000000000000000000000000000000..805c8bb5e4fd43a12a5891eea5a68788309629b0 GIT binary patch literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX 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