From d65f8bc03f4506e22f4410c5994ea6ab6eda9a62 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 12 May 2021 11:48:06 +0200 Subject: [PATCH 001/235] feat: add gatk4/bettointervallist module --- .../gatk4/bedtointervallist/functions.nf | 70 +++++++++++++++++++ .../software/gatk4/bedtointervallist/main.nf | 41 +++++++++++ .../software/gatk4/bedtointervallist/meta.yml | 39 +++++++++++ 3 files changed, 150 insertions(+) create mode 100644 modules/nf-core/software/gatk4/bedtointervallist/functions.nf create mode 100644 modules/nf-core/software/gatk4/bedtointervallist/main.nf create mode 100644 modules/nf-core/software/gatk4/bedtointervallist/meta.yml diff --git a/modules/nf-core/software/gatk4/bedtointervallist/functions.nf b/modules/nf-core/software/gatk4/bedtointervallist/functions.nf new file mode 100644 index 00000000..9d0137e3 --- /dev/null +++ b/modules/nf-core/software/gatk4/bedtointervallist/functions.nf @@ -0,0 +1,70 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/bedtointervallist/main.nf b/modules/nf-core/software/gatk4/bedtointervallist/main.nf new file mode 100644 index 00000000..284f2dfc --- /dev/null +++ b/modules/nf-core/software/gatk4/bedtointervallist/main.nf @@ -0,0 +1,41 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_BEDTOINTERVALLIST { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + } else { + container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + } + + input: + tuple val(meta), path(bed) + path sequence_dict + + output: + tuple val(meta), path('*.interval_list'), emit: interval_list + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + """ + gatk BedToIntervalList \\ + -I $bed \\ + -SD $sequence_dict \\ + -O ${prefix}.interval_list \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/bedtointervallist/meta.yml b/modules/nf-core/software/gatk4/bedtointervallist/meta.yml new file mode 100644 index 00000000..23e98447 --- /dev/null +++ b/modules/nf-core/software/gatk4/bedtointervallist/meta.yml @@ -0,0 +1,39 @@ +name: gatk4_bedtointervallist +description: Creates an interval list from a bed file and a reference dict +keywords: + - bed + - interval list +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - bed: + type: file + description: Input bed file + pattern: "*.bed" + - dict: + type: file + description: Sequence dictionary + pattern: "*.dict" +output: + - interval_list: + type: file + description: gatk interval list file + pattern: "*.interval_list" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" From 8e371073c33c8a05b35f6fe192cf75cd275ebf89 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 4 Jun 2021 16:54:43 +0200 Subject: [PATCH 002/235] sample workflow add with indexing and alignment with star --- assets/samplesheet.valid.csv | 2 + backup/nextflow.config | 192 ++++ bin/check_samplesheet.py | 136 +++ bin/fasta2gtf.py | 77 ++ bin/gtf2bed | 122 +++ conf/base.config | 87 +- conf/igenomes.config | 833 +++++++++--------- conf/modules.config | 250 ++++++ conf/test.config | 67 +- conf/test_full.config | 35 +- conf/test_sra.config | 24 + lib/NfcoreSchema.groovy | 525 +++++------ lib/NfcoreTemplate.groovy | 294 +++++++ lib/Utils.groovy | 47 + lib/WorkflowMain.groovy | 93 ++ lib/WorkflowRnaseq.groovy | 263 ++++++ lib/WorkflowSraDownload.groovy | 21 + main.nf | 391 +------- modules/local/cat_additional_fasta.nf | 39 + modules/local/functions.nf | 68 ++ modules/local/get_chrom_sizes.nf | 34 + modules/local/gtf2bed.nf | 30 + modules/local/gtf_gene_filter.nf | 30 + modules/local/samplesheet_check.nf | 31 + .../nf-core/software/cat/fastq/functions.nf | 68 ++ modules/nf-core/software/cat/fastq/main.nf | 47 + modules/nf-core/software/cat/fastq/meta.yml | 33 + modules/nf-core/software/gffread/functions.nf | 68 ++ modules/nf-core/software/gffread/main.nf | 34 + modules/nf-core/software/gffread/meta.yml | 43 + modules/nf-core/software/gunzip/functions.nf | 68 ++ modules/nf-core/software/gunzip/main.nf | 35 + modules/nf-core/software/gunzip/meta.yml | 27 + .../software/hisat2/build/functions.nf | 68 ++ modules/nf-core/software/hisat2/build/main.nf | 70 ++ .../nf-core/software/hisat2/build/meta.yml | 42 + .../hisat2/extractsplicesites/functions.nf | 68 ++ .../hisat2/extractsplicesites/main.nf | 36 + .../hisat2/extractsplicesites/meta.yml | 34 + .../rsem/preparereference/functions.nf | 68 ++ .../software/rsem/preparereference/main.nf | 67 ++ .../software/salmon/index/functions.nf | 68 ++ modules/nf-core/software/salmon/index/main.nf | 51 ++ .../nf-core/software/salmon/index/meta.yml | 35 + .../software/samtools/flagstat/functions.nf | 68 ++ .../software/samtools/flagstat/main.nf | 34 + .../software/samtools/flagstat/meta.yml | 48 + .../software/samtools/idxstats/functions.nf | 68 ++ .../software/samtools/idxstats/main.nf | 34 + .../software/samtools/idxstats/meta.yml | 49 ++ .../software/samtools/index/functions.nf | 68 ++ .../nf-core/software/samtools/index/main.nf | 35 + .../nf-core/software/samtools/index/meta.yml | 47 + .../software/samtools/sort/functions.nf | 68 ++ .../nf-core/software/samtools/sort/main.nf | 35 + .../nf-core/software/samtools/sort/meta.yml | 43 + .../software/samtools/stats/functions.nf | 68 ++ .../nf-core/software/samtools/stats/main.nf | 34 + .../nf-core/software/samtools/stats/meta.yml | 47 + .../nf-core/software/star/align/functions.nf | 68 ++ modules/nf-core/software/star/align/main.nf | 71 ++ modules/nf-core/software/star/align/meta.yml | 75 ++ .../software/star/genomegenerate/functions.nf | 68 ++ .../software/star/genomegenerate/main.nf | 67 ++ .../software/star/genomegenerate/meta.yml | 36 + modules/nf-core/software/untar/functions.nf | 68 ++ modules/nf-core/software/untar/main.nf | 35 + modules/nf-core/software/untar/meta.yml | 27 + nextflow.config | 367 ++++---- nextflow_schema.json | 661 +++++++++++--- subworkflows/local/input_check.nf | 43 + subworkflows/local/prepare_genome.nf | 215 +++++ subworkflows/nf-core/align_star.nf | 49 ++ subworkflows/nf-core/bam_sort_samtools.nf | 45 + subworkflows/nf-core/bam_stats_samtools.nf | 25 + workflows/rnaseq.nf | 145 +++ 76 files changed, 6105 insertions(+), 1427 deletions(-) create mode 100644 assets/samplesheet.valid.csv create mode 100644 backup/nextflow.config create mode 100755 bin/check_samplesheet.py create mode 100755 bin/fasta2gtf.py create mode 100755 bin/gtf2bed create mode 100644 conf/modules.config create mode 100644 conf/test_sra.config create mode 100755 lib/NfcoreTemplate.groovy create mode 100755 lib/Utils.groovy create mode 100755 lib/WorkflowMain.groovy create mode 100755 lib/WorkflowRnaseq.groovy create mode 100755 lib/WorkflowSraDownload.groovy create mode 100644 modules/local/cat_additional_fasta.nf create mode 100644 modules/local/functions.nf create mode 100644 modules/local/get_chrom_sizes.nf create mode 100644 modules/local/gtf2bed.nf create mode 100644 modules/local/gtf_gene_filter.nf create mode 100644 modules/local/samplesheet_check.nf create mode 100644 modules/nf-core/software/cat/fastq/functions.nf create mode 100644 modules/nf-core/software/cat/fastq/main.nf create mode 100644 modules/nf-core/software/cat/fastq/meta.yml create mode 100644 modules/nf-core/software/gffread/functions.nf create mode 100644 modules/nf-core/software/gffread/main.nf create mode 100644 modules/nf-core/software/gffread/meta.yml create mode 100644 modules/nf-core/software/gunzip/functions.nf create mode 100644 modules/nf-core/software/gunzip/main.nf create mode 100644 modules/nf-core/software/gunzip/meta.yml create mode 100644 modules/nf-core/software/hisat2/build/functions.nf create mode 100644 modules/nf-core/software/hisat2/build/main.nf create mode 100644 modules/nf-core/software/hisat2/build/meta.yml create mode 100644 modules/nf-core/software/hisat2/extractsplicesites/functions.nf create mode 100644 modules/nf-core/software/hisat2/extractsplicesites/main.nf create mode 100644 modules/nf-core/software/hisat2/extractsplicesites/meta.yml create mode 100644 modules/nf-core/software/rsem/preparereference/functions.nf create mode 100644 modules/nf-core/software/rsem/preparereference/main.nf create mode 100644 modules/nf-core/software/salmon/index/functions.nf create mode 100644 modules/nf-core/software/salmon/index/main.nf create mode 100644 modules/nf-core/software/salmon/index/meta.yml create mode 100644 modules/nf-core/software/samtools/flagstat/functions.nf create mode 100644 modules/nf-core/software/samtools/flagstat/main.nf create mode 100644 modules/nf-core/software/samtools/flagstat/meta.yml create mode 100644 modules/nf-core/software/samtools/idxstats/functions.nf create mode 100644 modules/nf-core/software/samtools/idxstats/main.nf create mode 100644 modules/nf-core/software/samtools/idxstats/meta.yml create mode 100644 modules/nf-core/software/samtools/index/functions.nf create mode 100644 modules/nf-core/software/samtools/index/main.nf create mode 100644 modules/nf-core/software/samtools/index/meta.yml create mode 100644 modules/nf-core/software/samtools/sort/functions.nf create mode 100644 modules/nf-core/software/samtools/sort/main.nf create mode 100644 modules/nf-core/software/samtools/sort/meta.yml create mode 100644 modules/nf-core/software/samtools/stats/functions.nf create mode 100644 modules/nf-core/software/samtools/stats/main.nf create mode 100644 modules/nf-core/software/samtools/stats/meta.yml create mode 100644 modules/nf-core/software/star/align/functions.nf create mode 100644 modules/nf-core/software/star/align/main.nf create mode 100644 modules/nf-core/software/star/align/meta.yml create mode 100644 modules/nf-core/software/star/genomegenerate/functions.nf create mode 100644 modules/nf-core/software/star/genomegenerate/main.nf create mode 100644 modules/nf-core/software/star/genomegenerate/meta.yml create mode 100644 modules/nf-core/software/untar/functions.nf create mode 100644 modules/nf-core/software/untar/main.nf create mode 100644 modules/nf-core/software/untar/meta.yml create mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/prepare_genome.nf create mode 100644 subworkflows/nf-core/align_star.nf create mode 100644 subworkflows/nf-core/bam_sort_samtools.nf create mode 100644 subworkflows/nf-core/bam_stats_samtools.nf create mode 100644 workflows/rnaseq.nf diff --git a/assets/samplesheet.valid.csv b/assets/samplesheet.valid.csv new file mode 100644 index 00000000..dd979a5d --- /dev/null +++ b/assets/samplesheet.valid.csv @@ -0,0 +1,2 @@ +sample,single_end,fastq_1,fastq_2,strandedness +WT_REP1_T1,0,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_2.fastq.gz,reverse diff --git a/backup/nextflow.config b/backup/nextflow.config new file mode 100644 index 00000000..e268676e --- /dev/null +++ b/backup/nextflow.config @@ -0,0 +1,192 @@ +/* + * ------------------------------------------------- + * nf-core/rnavar Nextflow config file + * ------------------------------------------------- + * Default config options for all environments. + */ + +// Global default params, used in configs +params { + + // Workflow flags + // TODO nf-core: Specify your pipeline's command line flags + genome = false + input = null + input_paths = null + single_end = false + outdir = './results' + publish_dir_mode = 'copy' + + // Boilerplate options + multiqc_config = false + email = false + email_on_fail = false + max_multiqc_email_size = 25.MB + plaintext_email = false + monochrome_logs = false + help = false + igenomes_base = 's3://ngi-igenomes/igenomes' + tracedir = "${params.outdir}/pipeline_info" + igenomes_ignore = false + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + hostnames = false + config_profile_name = null + config_profile_description = false + config_profile_contact = false + config_profile_url = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,input_paths' + + // Defaults only, expecting to be overwritten + max_memory = 128.GB + max_cpus = 16 + max_time = 240.h + +} + +// Container slug. Stable releases should specify release tag! +// Developmental code should specify :dev +process.container = 'nfcore/rnavar:dev' + +// Load base.config by default for all pipelines +includeConfig 'conf/base.config' + +// Load nf-core custom profiles from different Institutions +try { + includeConfig "${params.custom_config_base}/nfcore_custom.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") +} + +profiles { + conda { + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + process.conda = "$projectDir/environment.yml" + } + debug { process.beforeScript = 'echo $HOSTNAME' } + docker { + docker.enabled = true + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + // Avoid this error: + // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. + // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 + // once this is established and works well, nextflow might implement this behavior as new default. + docker.runOptions = '-u \$(id -u):\$(id -g)' + } + singularity { + docker.enabled = false + singularity.enabled = true + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + singularity.autoMounts = true + } + podman { + singularity.enabled = false + docker.enabled = false + podman.enabled = true + shifter.enabled = false + charliecloud.enabled = false + } + shifter { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = true + charliecloud.enabled = false + } + charliecloud { + singularity.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = true + } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } +} + +// Load igenomes.config if required +if (!params.igenomes_ignore) { + includeConfig 'conf/igenomes.config' +} + +// Export these variables to prevent local Python/R libraries from conflicting with those in the container +env { + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" +} + +// Capture exit codes from upstream processes when piping +process.shell = ['/bin/bash', '-euo', 'pipefail'] + +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') +timeline { + enabled = true + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" +} +report { + enabled = true + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" +} +trace { + enabled = true + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" +} +dag { + enabled = true + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" +} + +manifest { + name = 'nf-core/rnavar' + author = 'Maxime U Garcia' + homePage = 'https://github.com/nf-core/rnavar' + description = 'gatk4 rna variant calling' + mainScript = 'main.nf' + nextflowVersion = '>=20.04.0' + version = '1.0dev' +} + +// Function to ensure that resource requirements don't go beyond +// a maximum limit +def check_max(obj, type) { + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } + } +} diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py new file mode 100755 index 00000000..d7480a07 --- /dev/null +++ b/bin/check_samplesheet.py @@ -0,0 +1,136 @@ +#!/usr/bin/env python + +import os +import sys +import errno +import argparse + +def parse_args(args=None): + Description = "Reformat nf-core/rnaseq samplesheet file and check its contents." + Epilog = "Example usage: python check_samplesheet.py " + + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") + return parser.parse_args(args) + +def make_dir(path): + if len(path) > 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise exception + +def print_error(error, context='Line', context_str=''): + error_str = f"ERROR: Please check samplesheet -> {error}" + if context != '' and context_str != '': + error_str = f"ERROR: Please check samplesheet -> {error}\n{context.strip()}: '{context_str.strip()}'" + print(error_str) + sys.exit(1) + +def check_samplesheet(file_in, file_out): + """ + This function checks that the samplesheet follows the following structure: + + sample,fastq_1,fastq_2,strandedness + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,,forward + + For an example see: + https://github.com/nf-core/test-datasets/blob/rnaseq/samplesheet/v3.1/samplesheet_test.csv + """ + + sample_mapping_dict = {} + with open(file_in, "r") as fin: + + ## Check header + MIN_COLS = 3 + HEADER = ['sample', 'fastq_1', 'fastq_2', 'strandedness'] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[: len(HEADER)] != HEADER: + print(f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}") + sys.exit(1) + + ## Check sample entries + for line in fin: + lspl = [x.strip().strip('"') for x in line.strip().split(",")] + + ## Check valid number of columns per row + if len(lspl) < len(HEADER): + print_error(f"Invalid number of columns (minimum = {len(HEADER)})!", 'Line', line) + + num_cols = len([x for x in lspl if x]) + if num_cols < MIN_COLS: + print_error(f"Invalid number of populated columns (minimum = {MIN_COLS})!", 'Line', line) + + ## Check sample name entries + sample, fastq_1, fastq_2, strandedness = lspl[:len(HEADER)] + if sample: + if sample.find(" ") != -1: + print_error("Sample entry contains spaces!", "Line", line) + else: + print_error("Sample entry has not been specified!", "Line", line) + + ## Check FastQ file extension + for fastq in [fastq_1, fastq_2]: + if fastq: + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", "Line", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", 'Line', line) + + ## Check strandedness + strandednesses = ['unstranded', 'forward', 'reverse'] + if strandedness: + if strandedness not in strandednesses: + print_error(f"Strandedness must be one of '{', '.join(strandednesses)}'!", 'Line', line) + else: + print_error(f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.", 'Line', line) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2, strandedness] + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ['0', fastq_1, fastq_2, strandedness] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ['1', fastq_1, fastq_2, strandedness] + else: + print_error("Invalid combination of columns provided!", 'Line', line) + + ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, strandedness ]]} + if sample not in sample_mapping_dict: + sample_mapping_dict[sample] = [sample_info] + else: + if sample_info in sample_mapping_dict[sample]: + print_error("Samplesheet contains duplicate rows!", 'Line', line) + else: + sample_mapping_dict[sample].append(sample_info) + + ## Write validated samplesheet with appropriate columns + if len(sample_mapping_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + with open(file_out, "w") as fout: + fout.write(",".join(['sample', 'single_end', 'fastq_1', 'fastq_2', 'strandedness']) + "\n") + for sample in sorted(sample_mapping_dict.keys()): + + ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): + print_error(f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!", "Sample", sample) + + ## Check that multiple runs of the same sample are of the same strandedness + if not all(x[-1] == sample_mapping_dict[sample][0][-1] for x in sample_mapping_dict[sample]): + print_error(f"Multiple runs of a sample must have the same strandedness!", "Sample", sample) + + for idx,val in enumerate(sample_mapping_dict[sample]): + fout.write(','.join([f"{sample}_T{idx+1}"] + val) + '\n') + else: + print_error(f"No entries to process!","Samplesheet: {file_in}") + +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN, args.FILE_OUT) + +if __name__ == "__main__": + sys.exit(main()) diff --git a/bin/fasta2gtf.py b/bin/fasta2gtf.py new file mode 100755 index 00000000..197ba12c --- /dev/null +++ b/bin/fasta2gtf.py @@ -0,0 +1,77 @@ +#!/usr/bin/env python +""" +Read a custom fasta file and create a custom GTF containing each entry +""" +import argparse +from itertools import groupby +import logging + +# Create a logger +logging.basicConfig(format='%(name)s - %(asctime)s %(levelname)s: %(message)s') +logger = logging.getLogger(__file__) +logger.setLevel(logging.INFO) + + +def fasta_iter(fasta_name): + """ + modified from Brent Pedersen + Correct Way To Parse A Fasta File In Python + given a fasta file. yield tuples of header, sequence + + Fasta iterator from https://www.biostars.org/p/710/#120760 + """ + with open(fasta_name) as fh: + # ditch the boolean (x[0]) and just keep the header or sequence since + # we know they alternate. + faiter = (x[1] for x in groupby(fh, lambda line: line[0] == ">")) + for header in faiter: + # drop the ">" + headerStr = header.__next__()[1:].strip() + + # join all sequence lines to one. + seq = "".join(s.strip() for s in faiter.__next__()) + + yield (headerStr, seq) + + +def fasta2gtf(fasta, output, biotype): + fiter = fasta_iter(fasta) + # GTF output lines + lines = [] + attributes = \ + 'exon_id "{name}.1"; exon_number "1";{biotype} gene_id "{name}_gene"; gene_name "{name}_gene"; gene_source "custom"; transcript_id "{name}_gene"; transcript_name "{name}_gene";\n' + line_template = \ + "{name}\ttransgene\texon\t1\t{length}\t.\t+\t.\t" + attributes + + for ff in fiter: + name, seq = ff + # Use first ID as separated by spaces as the "sequence name" + # (equivalent to "chromosome" in other cases) + seqname = name.split()[0] + # Remove all spaces + name = seqname.replace(' ', '_') + length = len(seq) + biotype_attr = '' + if biotype: + biotype_attr = f' {biotype} "transgene";' + line = line_template.format( + name=name, length=length, biotype=biotype_attr) + lines.append(line) + + with open(output, 'w') as f: + f.write(''.join(lines)) + + +if __name__ == "__main__": + parser = argparse.ArgumentParser( + description="""Convert a custom fasta (e.g. transgene) + to a GTF annotation.""") + parser.add_argument("fasta", type=str, help="Custom transgene sequence") + parser.add_argument( + "-o", "--output", dest='output', + default='transgenes.gtf', type=str, help="Gene annotation GTF output") + parser.add_argument( + "-b", "--biotype", dest='biotype', + default='', type=str, help="Name of gene biotype attribute to use in last column of GTF entry") + args = parser.parse_args() + fasta2gtf(args.fasta, args.output, args.biotype) diff --git a/bin/gtf2bed b/bin/gtf2bed new file mode 100755 index 00000000..4da5c046 --- /dev/null +++ b/bin/gtf2bed @@ -0,0 +1,122 @@ +#!/usr/bin/env perl + +# Copyright (c) 2011 Erik Aronesty (erik@q32.com) +# +# Permission is hereby granted, free of charge, to any person obtaining a copy +# of this software and associated documentation files (the "Software"), to deal +# in the Software without restriction, including without limitation the rights +# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +# copies of the Software, and to permit persons to whom the Software is +# furnished to do so, subject to the following conditions: +# +# The above copyright notice and this permission notice shall be included in +# all copies or substantial portions of the Software. +# +# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +# THE SOFTWARE. +# +# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT. + +use Getopt::Long; + +my $extended; +GetOptions("x"=>\$extended); + +$in = shift @ARGV; + +my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n"; +open IN, $in_cmd; + +while () { + $gff = 2 if /^##gff-version 2/; + $gff = 3 if /^##gff-version 3/; + next if /^#/ && $gff; + + s/\s+$//; + # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr + my @f = split /\t/; + if ($gff) { + # most ver 2's stick gene names in the id field + ($id) = $f[8]=~ /\bID="([^"]+)"/; + # most ver 3's stick unquoted names in the name field + ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3; + } else { + ($id) = $f[8]=~ /transcript_id "([^"]+)"/; + } + + next unless $id && $f[0]; + + if ($f[2] eq 'exon') { + die "no position at exon on line $." if ! $f[3]; + # gff3 puts :\d in exons sometimes + $id =~ s/:\d+$// if $gff == 3; + push @{$exons{$id}}, \@f; + # save lowest start + $trans{$id} = \@f if !$trans{$id}; + } elsif ($f[2] eq 'start_codon') { + #optional, output codon start/stop as "thick" region in bed + $sc{$id}->[0] = $f[3]; + } elsif ($f[2] eq 'stop_codon') { + $sc{$id}->[1] = $f[4]; + } elsif ($f[2] eq 'miRNA' ) { + $trans{$id} = \@f if !$trans{$id}; + push @{$exons{$id}}, \@f; + } +} + +for $id ( + # sort by chr then pos + sort { + $trans{$a}->[0] eq $trans{$b}->[0] ? + $trans{$a}->[3] <=> $trans{$b}->[3] : + $trans{$a}->[0] cmp $trans{$b}->[0] + } (keys(%trans)) ) { + my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}}; + my ($cds, $cde); + ($cds, $cde) = @{$sc{$id}} if $sc{$id}; + + # sort by pos + my @ex = sort { + $a->[3] <=> $b->[3] + } @{$exons{$id}}; + + my $beg = $ex[0][3]; + my $end = $ex[-1][4]; + + if ($dir eq '-') { + # swap + $tmp=$cds; + $cds=$cde; + $cde=$tmp; + $cds -= 2 if $cds; + $cde += 2 if $cde; + } + + # not specified, just use exons + $cds = $beg if !$cds; + $cde = $end if !$cde; + + # adjust start for bed + --$beg; --$cds; + + my $exn = @ex; # exon count + my $exst = join ",", map {$_->[3]-$beg-1} @ex; # exon start + my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex; # exon size + + my $gene_id; + my $extend = ""; + if ($extended) { + ($gene_id) = $attr =~ /gene_name "([^"]+)"/; + ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id; + $extend="\t$gene_id"; + } + # added an extra comma to make it look exactly like ucsc's beds + print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n"; +} + +close IN; diff --git a/conf/base.config b/conf/base.config index ce9b8d4c..4945e74c 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,51 +1,50 @@ /* - * ------------------------------------------------- - * nf-core/rnavar Nextflow base config file - * ------------------------------------------------- - * A 'blank slate' config file, appropriate for general - * use on most high performace compute environments. - * Assumes that all software is installed and available - * on the PATH. Runs in `local` mode - all jobs will be - * run on the logged in environment. - */ +======================================================================================== + nf-core/rnaseq Nextflow base config file +======================================================================================== + A 'blank slate' config file, appropriate for general use on most high performance + compute environments. Assumes that all software is installed and available on + the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. +---------------------------------------------------------------------------------------- +*/ process { - // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 7.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' - - // Process-specific resource requirements - // NOTE - Only one of the labels below are used in the fastqc process in the main script. - // If possible, it would be nice to keep the same label naming convention when - // adding in your processes. - // TODO nf-core: Customise requirements for specific processes. - // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors - withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 14.GB * task.attempt, 'memory' ) } - time = { check_max( 6.h * task.attempt, 'time' ) } - } - withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 42.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - } - withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 84.GB * task.attempt, 'memory' ) } - time = { check_max( 10.h * task.attempt, 'time' ) } - } - withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } - } - withName:get_software_versions { - cache = false - } + errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + maxRetries = 1 + maxErrors = '-1' + // Process-specific resource requirements + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withLabel:process_high_memory { + memory = { check_max( 200.GB * task.attempt, 'memory' ) } + } + withLabel:error_ignore { + errorStrategy = 'ignore' + } + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 + } } diff --git a/conf/igenomes.config b/conf/igenomes.config index 31b7ee61..a270df7f 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -1,421 +1,422 @@ /* - * ------------------------------------------------- - * Nextflow config file for iGenomes paths - * ------------------------------------------------- - * Defines reference genomes, using iGenome paths - * Can be used by any config that customises the base - * path using $params.igenomes_base / --igenomes_base - */ +======================================================================================== + Nextflow config file for iGenomes paths +======================================================================================== + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } } diff --git a/conf/modules.config b/conf/modules.config new file mode 100644 index 00000000..756e337e --- /dev/null +++ b/conf/modules.config @@ -0,0 +1,250 @@ +/* +======================================================================================== + Config file for defining DSL2 per module options +======================================================================================== + Available keys to override module options: + args = Additional arguments appended to command in module. + args2 = Second set of arguments appended to command in module (multi-tool modules). + args3 = Third set of arguments appended to command in module (multi-tool modules). + publish_dir = Directory to publish results. + publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path + If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path + If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" + is appended as a directory to "publish_dir" path + If publish_by_meta = false / null - No directories are appended to "publish_dir" path + publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + The value of "directory" is appended to the standard "publish_dir" path as defined above. + If publish_files = null (unspecified) - All files are published. + If publish_files = false - No files are published. + suffix = File name suffix for output files. +---------------------------------------------------------------------------------------- +*/ + +params { + modules { + 'sra_ids_to_runinfo' { + publish_dir = 'public_data' + publish_files = ['tsv':'runinfo'] + } + 'sra_runinfo_to_ftp' { + publish_dir = 'public_data' + publish_files = ['tsv':'runinfo'] + } + 'sra_fastq_ftp' { + publish_dir = 'public_data' + publish_files = ['fastq.gz':'', 'md5':'md5'] + args = '-C - --max-time 1200' + } + 'sra_to_samplesheet' { + publish_dir = 'public_data' + publish_files = false + } + 'sra_merge_samplesheet' { + publish_dir = 'public_data' + } + 'gffread' { + args = '--keep-exon-attrs -F -T' + publish_dir = 'genome' + } + 'cat_fastq' { + publish_dir = 'fastq' + } + 'fastqc' { + args = '--quiet' + } + 'trimgalore' { + args = '--fastqc' + publish_files = ['txt':'', 'html':'fastqc', 'zip':'fastqc'] + } + 'umitools_extract' { + args = '' + publish_files = ['log':''] + } + 'sortmerna' { + args = '--num_alignments 1 --fastx -v' + publish_files = ['log':''] + } + 'star_genomegenerate' { + publish_dir = 'genome/index' + } + 'star_align' { + args = '--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend' + publish_dir = "${params.aligner}" + publish_files = ['out':'log', 'tab':'log'] + } + 'star_salmon_quant' { + publish_dir = "${params.aligner}" + } + 'star_salmon_tximport' { + publish_dir = "${params.aligner}" + publish_by_meta = true + } + 'star_salmon_merge_counts' { + publish_dir = "${params.aligner}" + } + 'hisat2_build' { + publish_dir = 'genome/index' + } + 'hisat2_align' { + args = '--met-stderr --new-summary --dta' + publish_files = ['log':'log'] + } + 'rsem_preparereference' { + args = '--star' + publish_dir = 'genome/index' + } + 'rsem_calculateexpression' { + args = '--star --star-output-genome-bam --star-gzipped-read-file --estimate-rspd --seed 1' + publish_files = ['log':'log', 'stat':'', 'results':''] + publish_dir = "${params.aligner}" + } + 'rsem_merge_counts' { + publish_dir = "${params.aligner}" + } + 'samtools_sort_genome' { + suffix = '.sorted' + publish_files = [:] + publish_dir = "${params.aligner}" + } + 'samtools_index_genome' { + args = '' + suffix = '.sorted' + publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "${params.aligner}" + } + 'samtools_sort_transcriptome' { + suffix = '.transcriptome.sorted' + publish_files = false + } + 'samtools_index_transcriptome' { + suffix = '.transcriptome.sorted' + publish_files = false + } + 'umitools_dedup_genome' { + publish_files = ['tsv':'umitools'] + suffix = '.umi_dedup.sorted' + publish_dir = "${params.aligner}" + } + 'umitools_dedup_genome_samtools' { + args = '' + suffix = '.umi_dedup.sorted' + publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "${params.aligner}" + } + 'umitools_dedup_transcriptome' { + publish_files = ['tsv':'umitools'] + suffix = '.umi_dedup.transcriptome.sorted' + publish_dir = "${params.aligner}" + } + 'umitools_dedup_transcriptome_sort' { + args = '-n' + suffix = '.umi_dedup.transcriptome' + publish_files = false + } + 'salmon_index' { + args = '' + publish_dir = 'genome/index' + } + 'salmon_quant' { + args = '' + } + 'salmon_tximport' { + publish_by_meta = true + } + 'salmon_merge_counts' { + args = '' + } + 'preseq_lcextrap' { + args = '-verbose -bam -seed 1 -seg_len 100000000' + publish_files = ['txt':'', 'log':'log'] + publish_dir = "${params.aligner}/preseq" + } + 'picard_markduplicates' { + args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' + suffix = '.markdup.sorted' + publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] + publish_dir = "${params.aligner}" + } + 'picard_markduplicates_samtools' { + args = '' + suffix = '.markdup.sorted' + publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "${params.aligner}" + } + 'stringtie' { + args = '-v' + publish_dir = "${params.aligner}/stringtie" + } + 'subread_featurecounts' { + args = '-B -C' + publish_dir = "${params.aligner}/featurecounts" + } + 'multiqc_custom_biotype' { + publish_dir = "${params.aligner}/featurecounts" + } + 'bedtools_genomecov' { + args = '-split -du' + publish_files = false + } + 'ucsc_bedclip_sense' { + suffix = '.clip.sense' + publish_files = false + } + 'ucsc_bedclip_antisense' { + suffix = '.clip.antisense' + publish_files = false + } + 'ucsc_bedgraphtobigwig_sense' { + suffix = '.sense' + publish_dir = "${params.aligner}/bigwig" + } + 'ucsc_bedgraphtobigwig_antisense' { + suffix = '.antisense' + publish_dir = "${params.aligner}/bigwig" + } + 'deseq2_qc' { + args = "--id_col 1 --sample_suffix '' --outprefix deseq2 --count_col 3" + publish_files = ['RData':'', 'pca.vals.txt':'', 'plots.pdf':'', 'sample.dists.txt':'', 'size_factors':'', 'log':''] + publish_dir = "${params.aligner}/deseq2_qc" + } + 'qualimap_rnaseq' { + publish_dir = "${params.aligner}/qualimap" + } + 'dupradar' { + publish_files = [ + 'Dens.pdf':'scatter_plot', 'Boxplot.pdf':'box_plot', 'Hist.pdf':'histogram', + 'Matrix.txt':'gene_data', 'slope.txt':'intercepts_slope' + ] + publish_dir = "${params.aligner}/dupradar" + } + 'rseqc_bamstat' { + publish_dir = "${params.aligner}/rseqc/bam_stat" + } + 'rseqc_innerdistance' { + publish_files = ['txt':'txt', 'pdf':'pdf', '.r':'rscript'] + publish_dir = "${params.aligner}/rseqc/inner_distance" + } + 'rseqc_inferexperiment' { + publish_dir = "${params.aligner}/rseqc/infer_experiment" + } + 'rseqc_junctionannotation' { + publish_files = ['pdf':'pdf', '.r':'rscript', 'bed':'bed', 'xls':'xls', 'log':'log'] + publish_dir = "${params.aligner}/rseqc/junction_annotation" + } + 'rseqc_junctionsaturation' { + publish_files = ['pdf':'pdf', '.r':'rscript'] + publish_dir = "${params.aligner}/rseqc/junction_saturation" + } + 'rseqc_readdistribution' { + publish_dir = "${params.aligner}/rseqc/read_distribution" + } + 'rseqc_readduplication' { + publish_files = ['pdf':'pdf', '.r':'rscript', 'xls':'xls'] + publish_dir = "${params.aligner}/rseqc/read_duplication" + } + 'multiqc' { + args = '' + publish_dir = "multiqc/${params.aligner}" + } + } +} diff --git a/conf/test.config b/conf/test.config index 5feadf2f..e7610dd0 100644 --- a/conf/test.config +++ b/conf/test.config @@ -1,28 +1,47 @@ /* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run nf-core/rnavar -profile test, - */ +======================================================================================== + Nextflow config file for running minimal tests +======================================================================================== + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/rnaseq -profile test, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = 6.GB - max_time = 48.h - - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] - // Ignore `--input` as otherwise the parameter validation will throw an error - schema_ignore_params = 'genomes,input_paths,input' + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run CI + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h + + // Input data + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_test.csv' + + // Genome references + fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genome.fa' + gtf = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gtf.gz' + gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' + transcript_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/transcriptome.fasta' + additional_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/gfp.fa.gz' + + hisat2_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/hisat2.tar.gz' + star_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/star.tar.gz' + salmon_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/salmon.tar.gz' + rsem_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/rsem.tar.gz' + + // Other parameters + pseudo_aligner = 'salmon' + umitools_bc_pattern = 'NNNN' + + // When using RSEM, remove warning from STAR whilst building tiny indices + modules { + 'rsem_preparereference' { + args2 = "--genomeSAindexNbases 7" + } + } } diff --git a/conf/test_full.config b/conf/test_full.config index 458446d1..7ae71d23 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -1,24 +1,21 @@ /* - * ------------------------------------------------- - * Nextflow config file for running full-size tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a full size pipeline test. Use as follows: - * nextflow run nf-core/rnavar -profile test_full, - */ +======================================================================================== + Nextflow config file for running full-size tests +======================================================================================== + Defines input files and everything required to run a full size pipeline test. + + Use as follows: + nextflow run nf-core/rnaseq -profile test_full, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] - // Ignore `--input` as otherwise the parameter validation will throw an error - schema_ignore_params = 'genomes,input_paths,input' + // Parameters for full-size test + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_full.csv' + genome = 'GRCh37' + pseudo_aligner = 'salmon' } diff --git a/conf/test_sra.config b/conf/test_sra.config new file mode 100644 index 00000000..dc35d881 --- /dev/null +++ b/conf/test_sra.config @@ -0,0 +1,24 @@ +/* +======================================================================================== + Nextflow config file for running minimal tests +======================================================================================== + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/rnaseq -profile test_sra, + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Public data download test profile' + config_profile_description = 'Minimal test dataset to check pipeline function when downloading data via the ENA' + + // Limit resources so that this can run CI + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h + + // Input data + public_data_ids = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/public_database_ids.txt' +} diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 52ee7304..16986434 100644 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -1,6 +1,6 @@ -/* - * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. - */ +// +// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. +// import org.everit.json.schema.Schema import org.everit.json.schema.loader.SchemaLoader @@ -13,16 +13,23 @@ import groovy.json.JsonBuilder class NfcoreSchema { - /* - * Function to loop over all parameters defined in schema and check - * whether the given paremeters adhere to the specificiations - */ + // + // Resolve Schema path relative to main workflow directory + // + public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { + return "${workflow.projectDir}/${schema_filename}" + } + + // + // Function to loop over all parameters defined in schema and check + // whether the given parameters adhere to the specifications + // /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - private static void validateParameters(params, jsonSchema, log) { + public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { def has_error = false //=====================================================================// // Check for nextflow core params and unexpected params - def json = new File(jsonSchema).text + def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') def nf_params = [ // Options for base `nextflow` command @@ -125,36 +132,36 @@ class NfcoreSchema { //=====================================================================// // Validate parameters against the schema - InputStream inputStream = new File(jsonSchema).newInputStream() - JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream)) + InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() + JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) // Remove anything that's in params.schema_ignore_params - rawSchema = removeIgnoredParams(rawSchema, params) + raw_schema = removeIgnoredParams(raw_schema, params) - Schema schema = SchemaLoader.load(rawSchema) + Schema schema = SchemaLoader.load(raw_schema) // Clean the parameters def cleanedParams = cleanParameters(params) // Convert to JSONObject def jsonParams = new JsonBuilder(cleanedParams) - JSONObject paramsJSON = new JSONObject(jsonParams.toString()) + JSONObject params_json = new JSONObject(jsonParams.toString()) // Validate try { - schema.validate(paramsJSON) + schema.validate(params_json) } catch (ValidationException e) { println '' log.error 'ERROR: Validation of pipeline parameters failed!' JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, paramsJSON, log) + printExceptions(exceptionJSON, params_json, log) println '' has_error = true } // Check for unexpected parameters if (unexpectedParams.size() > 0) { - Map colors = log_colours(params.monochrome_logs) + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) println '' def warn_msg = 'Found unexpected parameters:' for (unexpectedParam in unexpectedParams) { @@ -170,266 +177,17 @@ class NfcoreSchema { } } - // Loop over nested exceptions and print the causingException - private static void printExceptions(exJSON, paramsJSON, log) { - def causingExceptions = exJSON['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (exJSON['pointerToViolation'] == '#') { - log.error "* ${exJSON['message']}" - } - // Error with specific param - else { - def param = exJSON['pointerToViolation'] - ~/^#\// - def param_val = paramsJSON[param].toString() - log.error "* --${param}: ${exJSON['message']} (${param_val})" - } - } - for (ex in causingExceptions) { - printExceptions(ex, paramsJSON, log) - } - } - - // Remove an element from a JSONArray - private static JSONArray removeElement(jsonArray, element){ - def list = [] - int len = jsonArray.length() - for (int i=0;i - if(rawSchema.keySet().contains('definitions')){ - rawSchema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) { - rawSchema.get("properties").remove(ignore_param) - } - if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { - def cleaned_required = removeElement(rawSchema.required, ignore_param) - rawSchema.put("required", cleaned_required) - } - } - return rawSchema - } - - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - /* - * This method tries to read a JSON params file - */ - private static LinkedHashMap params_load(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = params_read(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - private static Map log_colours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - static String dashed_line(monochrome_logs) { - Map colors = log_colours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - /* - Method to actually read in JSON file using Groovy. - Group (as Key), values are all parameters - - Parameter1 as Key, Description as Value - - Parameter2 as Key, Description as Value - .... - Group - - - */ - private static LinkedHashMap params_read(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - /* - * Get maximum number of characters across all parameter names - */ - private static Integer params_max_chars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } - - /* - * Beautify parameters for --help - */ - private static String params_help(workflow, params, json_schema, command) { - Map colors = log_colours(params.monochrome_logs) + // + // Beautify parameters for --help + // + public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) Integer num_hidden = 0 String output = '' output += 'Typical pipeline command:\n\n' output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = params_load(json_schema) - Integer max_chars = params_max_chars(params_map) + 1 + Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + Integer max_chars = paramsMaxChars(params_map) + 1 Integer desc_indent = max_chars + 14 Integer dec_linewidth = 160 - desc_indent for (group in params_map.keySet()) { @@ -469,18 +227,17 @@ class NfcoreSchema { output += group_output } } - output += dashed_line(params.monochrome_logs) if (num_hidden > 0){ - output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset - output += dashed_line(params.monochrome_logs) + output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset } + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } - /* - * Groovy Map summarising parameters/workflow options used by the pipeline - */ - private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // + // Groovy Map summarising parameters/workflow options used by the pipeline + // + public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { // Get a selection of core Nextflow workflow options def Map workflow_summary = [:] if (workflow.revision) { @@ -503,7 +260,7 @@ class NfcoreSchema { // Get pipeline parameters defined in JSON Schema def Map params_summary = [:] def blacklist = ['hostnames'] - def params_map = params_load(json_schema) + def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) for (group in params_map.keySet()) { def sub_params = new LinkedHashMap() def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -546,14 +303,14 @@ class NfcoreSchema { return [ 'Core Nextflow options' : workflow_summary ] << params_summary } - /* - * Beautify parameters for summary and return as string - */ - private static String params_summary_log(workflow, params, json_schema) { - Map colors = log_colours(params.monochrome_logs) + // + // Beautify parameters for summary and return as string + // + public static String paramsSummaryLog(workflow, params) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) String output = '' - def params_map = params_summary_map(workflow, params, json_schema) - def max_chars = params_max_chars(params_map) + def params_map = paramsSummaryMap(workflow, params) + def max_chars = paramsMaxChars(params_map) for (group in params_map.keySet()) { def group_params = params_map.get(group) // This gets the parameters of that particular group if (group_params) { @@ -564,10 +321,196 @@ class NfcoreSchema { output += '\n' } } - output += dashed_line(params.monochrome_logs) - output += colors.dim + "\n Only displaying parameters that differ from defaults.\n" + colors.reset - output += dashed_line(params.monochrome_logs) + output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } + // + // Loop over nested exceptions and print the causingException + // + private static void printExceptions(ex_json, params_json, log) { + def causingExceptions = ex_json['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (ex_json['pointerToViolation'] == '#') { + log.error "* ${ex_json['message']}" + } + // Error with specific param + else { + def param = ex_json['pointerToViolation'] - ~/^#\// + def param_val = params_json[param].toString() + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, params_json, log) + } + } + + // + // Remove an element from a JSONArray + // + private static JSONArray removeElement(json_array, element) { + def list = [] + int len = json_array.length() + for (int i=0;i + if(raw_schema.keySet().contains('definitions')){ + raw_schema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { + raw_schema.get("properties").remove(ignore_param) + } + if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { + def cleaned_required = removeElement(raw_schema.required, ignore_param) + raw_schema.put("required", cleaned_required) + } + } + return raw_schema + } + + // + // Clean and check parameters relative to Nextflow native classes + // + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + // + // This function tries to read a JSON params file + // + private static LinkedHashMap paramsLoad(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = paramsRead(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + // + // Method to actually read in JSON file using Groovy. + // Group (as Key), values are all parameters + // - Parameter1 as Key, Description as Value + // - Parameter2 as Key, Description as Value + // .... + // Group + // - + private static LinkedHashMap paramsRead(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + // + // Get maximum number of characters across all parameter names + // + private static Integer paramsMaxChars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy new file mode 100755 index 00000000..26f3dd46 --- /dev/null +++ b/lib/NfcoreTemplate.groovy @@ -0,0 +1,294 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Check params.hostnames + // + public static void hostName(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } + } + } + } + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[], fail_percent_mapped=[:]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (fail_percent_mapped.size() > 0) { + subject = "[$workflow.manifest.name] Partially successful (${fail_percent_mapped.size()} skipped): $workflow.runName" + } + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + email_fields['fail_percent_mapped'] = fail_percent_mapped.keySet() + email_fields['min_mapped_reads'] = params.min_mapped_reads + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success && !params.skip_multiqc) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log, fail_percent_mapped=[:], pass_percent_mapped=[:]) { + Map colors = logColours(params.monochrome_logs) + + def total_aln_count = pass_percent_mapped.size() + fail_percent_mapped.size() + if (pass_percent_mapped.size() > 0) { + def idx = 0 + def samp_aln = '' + for (samp in pass_percent_mapped) { + samp_aln += " ${samp.value}%: ${samp.key}\n" + idx += 1 + if (idx > 5) { + samp_aln += " ..see pipeline reports for full list\n" + break; + } + } + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} ${pass_percent_mapped.size()}/$total_aln_count samples passed STAR ${params.min_mapped_reads}% mapped threshold:\n${samp_aln}${colors.reset}-" + } + if (fail_percent_mapped.size() > 0) { + def samp_aln = '' + for (samp in fail_percent_mapped) { + samp_aln += " ${samp.value}%: ${samp.key}\n" + } + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} ${fail_percent_mapped.size()}/$total_aln_count samples skipped since they failed STAR ${params.min_mapped_reads}% mapped threshold:\n${samp_aln}${colors.reset}-" + } + + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + hostName(workflow, params, log) + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/Utils.groovy b/lib/Utils.groovy new file mode 100755 index 00000000..18173e98 --- /dev/null +++ b/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + + // + // Join module args with appropriate spacing + // + public static String joinModuleArgs(args_list) { + return ' ' + args_list.join(' ') + } +} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy new file mode 100755 index 00000000..085dbd3d --- /dev/null +++ b/lib/WorkflowMain.groovy @@ -0,0 +1,93 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/rnaseq pipeline +// + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.1400710\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + // + // Print help to screen if required + // + public static String help(workflow, params, log) { + def command = "nextflow run nf-core/rnaseq --input samplesheet.csv --genome GRCh37 -profile docker" + def help_string = '' + help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) + help_string += NfcoreSchema.paramsHelp(workflow, params, command) + help_string += '\n' + citation(workflow) + '\n' + help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) + return help_string + } + + // + // Print parameter summary log to screen + // + public static String paramsSummaryLog(workflow, params, log) { + def summary_log = '' + summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) + summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) + summary_log += '\n' + citation(workflow) + '\n' + summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) + return summary_log + } + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log) { + // Print help to screen if required + if (params.help) { + log.info help(workflow, params, log) + System.exit(0) + } + + // Validate workflow parameters via the JSON schema + if (params.validate_params) { + NfcoreSchema.validateParameters(workflow, params, log) + } + + // Print parameter summary log to screen + log.info paramsSummaryLog(workflow, params, log) + + // Check that conda channels are set-up correctly + if (params.enable_conda) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check the hostnames against configured profiles + NfcoreTemplate.hostName(workflow, params, log) + + // Check at least one form of input has been provided + if (!params.public_data_ids && !params.input) { + log.error "Please specify at least one form of input for the pipeline e.g. '--input samplsheet.csv' or '--public_data_ids ids.txt'." + System.exit(1) + } + } + + // + // Get attribute from genome config file e.g. fasta + // + public static String getGenomeAttribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} diff --git a/lib/WorkflowRnaseq.groovy b/lib/WorkflowRnaseq.groovy new file mode 100755 index 00000000..5cdc71ac --- /dev/null +++ b/lib/WorkflowRnaseq.groovy @@ -0,0 +1,263 @@ +// +// This file holds several functions specific to the workflow/rnaseq.nf in the nf-core/rnaseq pipeline +// + +class WorkflowRnaseq { + + // + // Check and validate parameters + // + public static void initialise(params, log, valid_params) { + genomeExistsError(params, log) + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + + if (!params.gtf && !params.gff) { + log.error "No GTF or GFF3 annotation specified! The pipeline requires at least one of these files." + System.exit(1) + } + + if (params.gtf) { + if (params.gff) { + gtfGffWarn(log) + } + if (params.genome == 'GRCh38' && params.gtf.contains('Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf')) { + ncbiGenomeWarn(log) + } + if (params.gtf.contains('/UCSC/') && params.gtf.contains('Annotation/Genes/genes.gtf')) { + ucscGenomeWarn(log) + } + } + + if (!params.skip_alignment) { + if (!valid_params['aligners'].contains(params.aligner)) { + log.error "Invalid option: '${params.aligner}'. Valid options for '--aligner': ${valid_params['aligners'].join(', ')}." + System.exit(1) + } + } else { + if (!params.pseudo_aligner) { + log.error "--skip_alignment specified without --pseudo_aligner...please specify e.g. --pseudo_aligner ${valid_params['pseudoaligners'][0]}." + System.exit(1) + } + skipAlignmentWarn(log) + } + + if (params.pseudo_aligner) { + if (!valid_params['pseudoaligners'].contains(params.pseudo_aligner)) { + log.error "Invalid option: '${params.pseudo_aligner}'. Valid options for '--pseudo_aligner': ${valid_params['pseudoaligners'].join(', ')}." + System.exit(1) + } else { + if (!(params.salmon_index || params.transcript_fasta || (params.fasta && (params.gtf || params.gff)))) { + log.error "To use `--pseudo_aligner 'salmon'`, you must provide either --salmon_index or --transcript_fasta or both --fasta and --gtf / --gff." + System.exit(1) + } + } + } + + // Checks when running --aligner star_rsem + if (!params.skip_alignment && params.aligner == 'star_rsem') { + if (params.with_umi) { + rsemUmiError(log) + } + if (params.rsem_index && params.star_index) { + rsemStarIndexWarn(log) + } + } + + // Check which RSeQC modules we are running + def rseqc_modules = params.rseqc_modules ? params.rseqc_modules.split(',').collect{ it.trim().toLowerCase() } : [] + if ((valid_params['rseqc_modules'] + rseqc_modules).unique().size() != valid_params['rseqc_modules'].size()) { + log.error "Invalid option: ${params.rseqc_modules}. Valid options for '--rseqc_modules': ${valid_params['rseqc_modules'].join(', ')}" + System.exit(1) + } + } + + // + // Function to check whether biotype field exists in GTF file + // + public static Boolean biotypeInGtf(gtf_file, biotype, log) { + def hits = 0 + gtf_file.eachLine { line -> + def attributes = line.split('\t')[-1].split() + if (attributes.contains(biotype)) { + hits += 1 + } + } + if (hits) { + return true + } else { + log.warn "=============================================================================\n" + + " Biotype attribute '${biotype}' not found in the last column of the GTF file!\n\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " Amend '--featurecounts_group_type' to change this behaviour.\n" + + "===================================================================================" + return false + } + } + + // + // Function that parses and returns the alignment rate from the STAR log output + // + public static ArrayList getStarPercentMapped(params, align_log) { + def percent_aligned = 0 + def pattern = /Uniquely mapped reads %\s*\|\s*([\d\.]+)%/ + align_log.eachLine { line -> + def matcher = line =~ pattern + if (matcher) { + percent_aligned = matcher[0][1].toFloat() + } + } + + def pass = false + if (percent_aligned >= params.min_mapped_reads.toFloat()) { + pass = true + } + return [ percent_aligned, pass ] + } + + // + // Function that parses and returns the predicted strandedness from the RSeQC infer_experiment.py output + // + public static ArrayList getInferexperimentStrandedness(inferexperiment_file, cutoff=30) { + def sense = 0 + def antisense = 0 + def undetermined = 0 + inferexperiment_file.eachLine { line -> + def undetermined_matcher = line =~ /Fraction of reads failed to determine:\s([\d\.]+)/ + def se_sense_matcher = line =~ /Fraction of reads explained by "\++,--":\s([\d\.]+)/ + def se_antisense_matcher = line =~ /Fraction of reads explained by "\+-,-\+":\s([\d\.]+)/ + def pe_sense_matcher = line =~ /Fraction of reads explained by "1\++,1--,2\+-,2-\+":\s([\d\.]+)/ + def pe_antisense_matcher = line =~ /Fraction of reads explained by "1\+-,1-\+,2\+\+,2--":\s([\d\.]+)/ + if (undetermined_matcher) undetermined = undetermined_matcher[0][1].toFloat() * 100 + if (se_sense_matcher) sense = se_sense_matcher[0][1].toFloat() * 100 + if (se_antisense_matcher) antisense = se_antisense_matcher[0][1].toFloat() * 100 + if (pe_sense_matcher) sense = pe_sense_matcher[0][1].toFloat() * 100 + if (pe_antisense_matcher) antisense = pe_antisense_matcher[0][1].toFloat() * 100 + } + def strandedness = 'unstranded' + if (sense >= 100-cutoff) { + strandedness = 'forward' + } else if (antisense >= 100-cutoff) { + strandedness = 'reverse' + } + return [ strandedness, sense, antisense, undetermined ] + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "===================================================================================" + System.exit(1) + } + } + + // + // Print a warning if using GRCh38 assembly from igenomes.config + // + private static void ncbiGenomeWarn(log) { + log.warn "=============================================================================\n" + + " When using '--genome GRCh38' the assembly is from the NCBI and NOT Ensembl.\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " If you would like to use the soft-masked Ensembl assembly instead please see:\n" + + " https://github.com/nf-core/rnaseq/issues/159#issuecomment-501184312\n" + + "===================================================================================" + } + + // + // Print a warning if using a UCSC assembly from igenomes.config + // + private static void ucscGenomeWarn(log) { + log.warn "=============================================================================\n" + + " When using UCSC assemblies the 'gene_biotype' field is absent from the GTF file.\n" + + " Biotype QC will be skipped to circumvent the issue below:\n" + + " https://github.com/nf-core/rnaseq/issues/460\n\n" + + " If you would like to use the soft-masked Ensembl assembly instead please see:\n" + + " https://github.com/nf-core/rnaseq/issues/159#issuecomment-501184312\n" + + "===================================================================================" + } + + // + // Print a warning if both GTF and GFF have been provided + // + private static void gtfGffWarn(log) { + log.warn "=============================================================================\n" + + " Both '--gtf' and '--gff' parameters have been provided.\n" + + " Using GTF file as priority.\n" + + "===================================================================================" + } + + // + // Print a warning if --skip_alignment has been provided + // + private static void skipAlignmentWarn(log) { + log.warn "=============================================================================\n" + + " '--skip_alignment' parameter has been provided.\n" + + " Skipping alignment, genome-based quantification and all downstream QC processes.\n" + + "===================================================================================" + } + + // + // Print a warning if using '--aligner star_rsem' and '--with_umi' + // + private static void rsemUmiError(log) { + log.error "=============================================================================\n" + + " When using '--aligner star_rsem', STAR is run by RSEM itself and so it is\n" + + " not possible to remove UMIs before the quantification.\n\n" + + " If you would like to remove UMI barcodes using the '--with_umi' option\n" + + " please use either '--aligner star_salmon' or '--aligner hisat2'.\n" + + "=============================================================================" + System.exit(1) + } + + // + // Print a warning if using '--aligner star_rsem' and providing both '--rsem_index' and '--star_index' + // + private static void rsemStarIndexWarn(log) { + log.warn "=============================================================================\n" + + " When using '--aligner star_rsem', both the STAR and RSEM indices should\n" + + " be present in the path specified by '--rsem_index'.\n\n" + + " This warning has been generated because you have provided both\n" + + " '--rsem_index' and '--star_index'. The pipeline will ignore the latter.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/568\n" + + "===================================================================================" + } +} diff --git a/lib/WorkflowSraDownload.groovy b/lib/WorkflowSraDownload.groovy new file mode 100755 index 00000000..a1470c18 --- /dev/null +++ b/lib/WorkflowSraDownload.groovy @@ -0,0 +1,21 @@ +// +// This file holds functions specific to the workflow/sra_download.nf in the nf-core/rnaseq pipeline +// + +class WorkflowSraDownload { + + // + // Print a warning after SRA download has completed + // + public static void sraDownloadWarn(log) { + log.warn "=============================================================================\n" + + " Please double-check the samplesheet that has been auto-created using the\n" + + " public database ids provided via the '--public_data_ids' parameter.\n\n" + + " Public databases don't reliably hold information such as strandedness\n" + + " information.\n\n" + + " All of the sample metadata obtained from the ENA has been appended\n" + + " as additional columns to help you manually curate the samplesheet before\n" + + " you run the main workflow(s) in the pipeline.\n" + + "===================================================================================" + } +} diff --git a/main.nf b/main.nf index 6953743b..c6a2f477 100644 --- a/main.nf +++ b/main.nf @@ -9,382 +9,41 @@ ---------------------------------------------------------------------------------------- */ -log.info Headers.nf_core(workflow, params.monochrome_logs) - -//////////////////////////////////////////////////// -/* -- PRINT HELP -- */ -////////////////////////////////////////////////////+ -def json_schema = "$projectDir/nextflow_schema.json" -if (params.help) { - def command = "nextflow run nf-core/rnavar --input '*_R{1,2}.fastq.gz' -profile docker" - log.info NfcoreSchema.params_help(workflow, params, json_schema, command) - exit 0 -} - -//////////////////////////////////////////////////// -/* -- VALIDATE PARAMETERS -- */ -////////////////////////////////////////////////////+ -if (params.validate_params) { - NfcoreSchema.validateParameters(params, json_schema, log) -} - -//////////////////////////////////////////////////// -/* -- Collect configuration parameters -- */ -//////////////////////////////////////////////////// - -// Check if genome exists in the config file -if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(', ')}" -} - -// TODO nf-core: Add any reference files that are needed -// Configurable reference genomes -// -// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// If you want to use the channel below in a process, define the following: -// input: -// file fasta from ch_fasta -// -params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false -if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } - -// Check AWS batch settings -if (workflow.profile.contains('awsbatch')) { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, 'Specify correct --awsqueue and --awsregion parameters on AWSBatch!' - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, 'Outdir not on S3 - specify S3 Bucket to run on AWSBatch!' - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, 'Specify a local tracedir or run without trace! S3 cannot be used for tracefiles.' -} - -// Stage config files -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() -ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) -ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) +nextflow.enable.dsl=2 /* - * Create a channel for input read files - */ -if (params.input_paths) { - if (params.single_end) { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } else { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, 'params.input_paths was empty - no input files supplied' } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } -} else { - Channel - .fromFilePairs(params.input, size: params.single_end ? 1 : 2) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } - .into { ch_read_files_fastqc; ch_read_files_trimming } -} - -//////////////////////////////////////////////////// -/* -- PRINT PARAMETER SUMMARY -- */ -//////////////////////////////////////////////////// -log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) - -// Header log info -def summary = [:] -if (workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Run Name'] = workflow.runName -// TODO nf-core: Report custom parameters here -summary['Input'] = params.input -summary['Fasta Ref'] = params.fasta -summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' -summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" -if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" -summary['Output dir'] = params.outdir -summary['Launch dir'] = workflow.launchDir -summary['Working dir'] = workflow.workDir -summary['Script dir'] = workflow.projectDir -summary['User'] = workflow.userName -if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli -} -summary['Config Profile'] = workflow.profile -if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description -if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact -if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url -summary['Config Files'] = workflow.configFiles.join(', ') -if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size -} - -// Check the hostnames against configured profiles -checkHostname() - -Channel.from(summary.collect{ [it.key, it.value] }) - .map { k,v -> "
$k
${v ?: 'N/A'}
" } - .reduce { a, b -> return [a, b].join("\n ") } - .map { x -> """ - id: 'nf-core-rnavar-summary' - description: " - this information is collected when the pipeline is started." - section_name: 'nf-core/rnavar Workflow Summary' - section_href: 'https://github.com/nf-core/rnavar' - plot_type: 'html' - data: | -
- $x -
- """.stripIndent() } - .set { ch_workflow_summary } - -/* - * Parse software version numbers - */ -process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: { filename -> - if (filename.indexOf('.csv') > 0) filename - else null - } - - output: - file 'software_versions_mqc.yaml' into ch_software_versions_yaml - file 'software_versions.csv' - - script: - // TODO nf-core: Get all tools to print their version number here - """ - echo $workflow.manifest.version > v_pipeline.txt - echo $workflow.nextflow.version > v_nextflow.txt - fastqc --version > v_fastqc.txt - multiqc --version > v_multiqc.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} - -/* - * STEP 1 - FastQC - */ -process fastqc { - tag "$name" - label 'process_medium' - publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, - saveAs: { filename -> - filename.indexOf('.zip') > 0 ? "zips/$filename" : "$filename" - } - - input: - set val(name), file(reads) from ch_read_files_fastqc +======================================================================================== +GENOME PARAMETER VALUES +======================================================================================== +*/ - output: - file '*_fastqc.{zip,html}' into ch_fastqc_results +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') +params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') +params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') +params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') - script: - """ - fastqc --quiet --threads $task.cpus $reads - """ -} /* - * STEP 2 - MultiQC - */ -process multiqc { - publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode - - input: - file (multiqc_config) from ch_multiqc_config - file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) - // TODO nf-core: Add in log files from your new processes for MultiQC to find! - file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from ch_software_versions_yaml.collect() - file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") - - output: - file "*multiqc_report.html" into ch_multiqc_report - file "*_data" - file "multiqc_plots" +======================================================================================== +VALIDATE AND PRINT PARAMETER SUMMARY +======================================================================================== +*/ - script: - rtitle = '' - rfilename = '' - if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - rtitle = "--title \"${workflow.runName}\"" - rfilename = "--filename " + workflow.runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" - } - custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' - // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time - """ - multiqc -f $rtitle $rfilename $custom_config_file . - """ -} +WorkflowMain.initialise(workflow, params, log) /* - * STEP 3 - Output Description HTML - */ -process output_documentation { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - file output_docs from ch_output_docs - file images from ch_output_docs_images - - output: - file 'results_description.html' +======================================================================================== +RUN THE WORKFLOW +======================================================================================== +*/ - script: - """ - markdown_to_html.py $output_docs -o results_description.html - """ +workflow { + include { RNASEQ_VAR } from './workflows/rnaseq' + RNASEQ_VAR () + } /* - * Completion e-mail notification - */ -workflow.onComplete { - - // Set up the e-mail variables - def subject = "[nf-core/rnavar] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[nf-core/rnavar] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = ch_multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[nf-core/rnavar] Found multiple reports from process 'multiqc', will use only one" - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[nf-core/rnavar] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[nf-core/rnavar] Sent summary e-mail to $email_address (sendmail)" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "[nf-core/rnavar] Sent summary e-mail to $email_address (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_red = params.monochrome_logs ? '' : "\033[0;31m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" - log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" - log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" - } - - if (workflow.success) { - log.info "-${c_purple}[nf-core/rnavar]${c_green} Pipeline completed successfully${c_reset}-" - } else { - checkHostname() - log.info "-${c_purple}[nf-core/rnavar]${c_red} Pipeline completed with errors${c_reset}-" - } - -} - -workflow.onError { - // Print unexpected parameters - easiest is to just rerun validation - NfcoreSchema.validateParameters(params, json_schema, log) -} - -def checkHostname() { - def c_reset = params.monochrome_logs ? '' : "\033[0m" - def c_white = params.monochrome_logs ? '' : "\033[0;37m" - def c_red = params.monochrome_logs ? '' : "\033[1;91m" - def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if (params.hostnames) { - def hostname = 'hostname'.execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "${c_red}====================================================${c_reset}\n" + - " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + - " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "${c_red}====================================================${c_reset}\n" - } - } - } - } -} +END OF THE FILE +*/ \ No newline at end of file diff --git a/modules/local/cat_additional_fasta.nf b/modules/local/cat_additional_fasta.nf new file mode 100644 index 00000000..a4fb6a11 --- /dev/null +++ b/modules/local/cat_additional_fasta.nf @@ -0,0 +1,39 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process CAT_ADDITIONAL_FASTA { + tag "$add_fasta" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path fasta + path gtf + path add_fasta + val biotype + + output: + path "${name}.fasta", emit: fasta + path "${name}.gtf" , emit: gtf + + script: + def genome_name = params.genome ? params.genome : fasta.getBaseName() + def biotype_name = biotype ? "-b $biotype" : '' + def add_name = add_fasta.getBaseName() + name = "${genome_name}_${add_name}" + """ + fasta2gtf.py -o ${add_fasta.baseName}.gtf $biotype_name $add_fasta + cat $fasta $add_fasta > ${name}.fasta + cat $gtf ${add_fasta.baseName}.gtf > ${name}.gtf + """ +} diff --git a/modules/local/functions.nf b/modules/local/functions.nf new file mode 100644 index 00000000..2e2a7956 --- /dev/null +++ b/modules/local/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf new file mode 100644 index 00000000..e20fb395 --- /dev/null +++ b/modules/local/get_chrom_sizes.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process GET_CHROM_SIZES { + tag "$fasta" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + path fasta + + output: + path '*.sizes' , emit: sizes + path '*.fai' , emit: fai + path "*.version.txt", emit: version + + script: + def software = 'samtools' + """ + samtools faidx $fasta + cut -f 1,2 ${fasta}.fai > ${fasta}.sizes + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf new file mode 100644 index 00000000..9bc1d85e --- /dev/null +++ b/modules/local/gtf2bed.nf @@ -0,0 +1,30 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process GTF2BED { + tag "$gtf" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/perl:5.26.2" + } else { + container "quay.io/biocontainers/perl:5.26.2" + } + + input: + path gtf + + output: + path '*.bed' + + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ + """ + gtf2bed $gtf > ${gtf.baseName}.bed + """ +} diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf new file mode 100644 index 00000000..d30ab21b --- /dev/null +++ b/modules/local/gtf_gene_filter.nf @@ -0,0 +1,30 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process GTF_GENE_FILTER { + tag "$fasta" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path fasta + path gtf + + output: + path "*.gtf" + + script: // filter_gtf_for_genes_in_genome.py is bundled with the pipeline, in nf-core/rnaseq/bin/ + """ + filter_gtf_for_genes_in_genome.py --gtf $gtf --fasta $fasta -o ${fasta.baseName}_genes.gtf + """ +} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf new file mode 100644 index 00000000..4b8410aa --- /dev/null +++ b/modules/local/samplesheet_check.nf @@ -0,0 +1,31 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +process SAMPLESHEET_CHECK { + tag "$samplesheet" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path samplesheet + + output: + path '*.csv' + + script: // This script is bundled with the pipeline, in nf-core/rnaseq/bin/ + """ + check_samplesheet.py \\ + $samplesheet \\ + samplesheet.valid.csv + """ +} diff --git a/modules/nf-core/software/cat/fastq/functions.nf b/modules/nf-core/software/cat/fastq/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/cat/fastq/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/cat/fastq/main.nf b/modules/nf-core/software/cat/fastq/main.nf new file mode 100644 index 00000000..55ccca90 --- /dev/null +++ b/modules/nf-core/software/cat/fastq/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process CAT_FASTQ { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.merged.fastq.gz"), emit: reads + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def readList = reads.collect{ it.toString() } + if (meta.single_end) { + if (readList.size > 1) { + """ + cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + """ + } + } else { + if (readList.size > 2) { + def read1 = [] + def read2 = [] + readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } + """ + cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + """ + } + } +} diff --git a/modules/nf-core/software/cat/fastq/meta.yml b/modules/nf-core/software/cat/fastq/meta.yml new file mode 100644 index 00000000..e7b8eebe --- /dev/null +++ b/modules/nf-core/software/cat/fastq/meta.yml @@ -0,0 +1,33 @@ +name: cat_fastq +description: Concatenates fastq files +keywords: + - fastq + - concatenate +tools: + - cat: + description: | + The cat utility reads files sequentially, writing them to the standard output. + documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FastQ files to be concatenated. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: Merged fastq file + pattern: "*.{merged.fastq.gz}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/software/gffread/functions.nf b/modules/nf-core/software/gffread/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gffread/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gffread/main.nf b/modules/nf-core/software/gffread/main.nf new file mode 100644 index 00000000..6c5aecbc --- /dev/null +++ b/modules/nf-core/software/gffread/main.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GFFREAD { + tag "$gff" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::gffread=0.12.1" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0" + } else { + container "quay.io/biocontainers/gffread:0.12.1--h8b12597_0" + } + + input: + path gff + + output: + path "*.gtf" , emit: gtf + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + """ + gffread $gff $options.args -o ${gff.baseName}.gtf + echo \$(gffread --version 2>&1) > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gffread/meta.yml b/modules/nf-core/software/gffread/meta.yml new file mode 100644 index 00000000..63d281f5 --- /dev/null +++ b/modules/nf-core/software/gffread/meta.yml @@ -0,0 +1,43 @@ +name: gffread +description: Validate, filter, convert and perform various other operations on GFF files +keywords: + - gff + - conversion + - validation +tools: + - gffread: + description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more. + homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread + documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread + tool_dev_url: https://github.com/gpertea/gffread + doi: 10.12688/f1000research.23297.1 + licence: ['MIT'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gff: + type: file + description: A GFF file in either the GFF3 or GFF2 format. + patter: "*.gff" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gtf: + type: file + description: GTF file resulting from the conversion of the GFF input file + pattern: "*.gtf" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@emiller88" diff --git a/modules/nf-core/software/gunzip/functions.nf b/modules/nf-core/software/gunzip/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gunzip/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gunzip/main.nf b/modules/nf-core/software/gunzip/main.nf new file mode 100644 index 00000000..29248796 --- /dev/null +++ b/modules/nf-core/software/gunzip/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GUNZIP { + tag "$archive" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + path archive + + output: + path "$gunzip", emit: gunzip + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + gunzip = archive.toString() - '.gz' + """ + gunzip -f $options.args $archive + echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gunzip/meta.yml b/modules/nf-core/software/gunzip/meta.yml new file mode 100644 index 00000000..922e74e6 --- /dev/null +++ b/modules/nf-core/software/gunzip/meta.yml @@ -0,0 +1,27 @@ +name: gunzip +description: Compresses and decompresses files. +keywords: + - gunzip + - compression +tools: + - gunzip: + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html +input: + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" +output: + - gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/software/hisat2/build/functions.nf b/modules/nf-core/software/hisat2/build/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/hisat2/build/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/hisat2/build/main.nf b/modules/nf-core/software/hisat2/build/main.nf new file mode 100644 index 00000000..ce8bfb26 --- /dev/null +++ b/modules/nf-core/software/hisat2/build/main.nf @@ -0,0 +1,70 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +def VERSION = '2.2.0' + +process HISAT2_BUILD { + tag "$fasta" + label 'process_high' + label 'process_high_memory' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::hisat2=2.2.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/hisat2:2.2.0--py37hfa133b6_4" + } else { + container "quay.io/biocontainers/hisat2:2.2.0--py37hfa133b6_4" + } + + input: + path fasta + path gtf + path splicesites + + output: + path "hisat2" , emit: index + path "*.version.txt", emit: version + + script: + def avail_mem = 0 + if (!task.memory) { + log.info "[HISAT2 index build] Available memory not known - defaulting to 0. Specify process memory requirements to change this." + } else { + log.info "[HISAT2 index build] Available memory: ${task.memory}" + avail_mem = task.memory.toGiga() + } + + def ss = '' + def exon = '' + def extract_exons = '' + def hisat2_build_memory = params.hisat2_build_memory ? (params.hisat2_build_memory as nextflow.util.MemoryUnit).toGiga() : 0 + if (avail_mem >= hisat2_build_memory) { + log.info "[HISAT2 index build] At least ${hisat2_build_memory} GB available, so using splice sites and exons to build HISAT2 index" + extract_exons = "hisat2_extract_exons.py $gtf > ${gtf.baseName}.exons.txt" + ss = "--ss $splicesites" + exon = "--exon ${gtf.baseName}.exons.txt" + } else { + log.info "[HISAT2 index build] Less than ${hisat2_build_memory} GB available, so NOT using splice sites and exons to build HISAT2 index." + log.info "[HISAT2 index build] Use --hisat2_build_memory [small number] to skip this check." + } + + def software = getSoftwareName(task.process) + """ + mkdir hisat2 + $extract_exons + hisat2-build \\ + -p $task.cpus \\ + $ss \\ + $exon \\ + $options.args \\ + $fasta \\ + hisat2/${fasta.baseName} + + echo $VERSION > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/hisat2/build/meta.yml b/modules/nf-core/software/hisat2/build/meta.yml new file mode 100644 index 00000000..2f34d9d8 --- /dev/null +++ b/modules/nf-core/software/hisat2/build/meta.yml @@ -0,0 +1,42 @@ +name: hisat2_build +description: Builds HISAT2 index for reference genome +keywords: + - build + - index + - fasta + - genome + - reference +tools: + - hisat2: + description: HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome. + homepage: https://daehwankimlab.github.io/hisat2/ + documentation: https://daehwankimlab.github.io/hisat2/manual/ + doi: "10.1038/s41587-019-0201-4" + licence: ['MIT'] + +input: + - fasta: + type: file + description: Reference fasta file + pattern: "*.{fa,fasta,fna}" + - gtf: + type: file + description: Reference gtf annotation file + pattern: "*.{gtf}" + - splicesites: + type: file + description: Splices sites in gtf file + pattern: "*.{txt}" + +output: + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - index: + type: file + description: HISAT2 genome index file + pattern: "*.ht2" + +authors: + - "@ntoda03" diff --git a/modules/nf-core/software/hisat2/extractsplicesites/functions.nf b/modules/nf-core/software/hisat2/extractsplicesites/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/hisat2/extractsplicesites/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/hisat2/extractsplicesites/main.nf b/modules/nf-core/software/hisat2/extractsplicesites/main.nf new file mode 100644 index 00000000..57f4dedb --- /dev/null +++ b/modules/nf-core/software/hisat2/extractsplicesites/main.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +def VERSION = '2.2.0' + +process HISAT2_EXTRACTSPLICESITES { + tag "$gtf" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::hisat2=2.2.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/hisat2:2.2.0--py37hfa133b6_4" + } else { + container "quay.io/biocontainers/hisat2:2.2.0--py37hfa133b6_4" + } + + input: + path gtf + + output: + path "*.splice_sites.txt", emit: txt + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + hisat2_extract_splice_sites.py $gtf > ${gtf.baseName}.splice_sites.txt + echo $VERSION > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/hisat2/extractsplicesites/meta.yml b/modules/nf-core/software/hisat2/extractsplicesites/meta.yml new file mode 100644 index 00000000..228138a8 --- /dev/null +++ b/modules/nf-core/software/hisat2/extractsplicesites/meta.yml @@ -0,0 +1,34 @@ +name: hisat2_extractsplicesites +description: Extracts splicing sites from a gtf files +keywords: + - splicing + - gtf + - genome + - reference + +tools: + - hisat2: + description: HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome. + homepage: https://daehwankimlab.github.io/hisat2/ + documentation: https://daehwankimlab.github.io/hisat2/manual/ + doi: "10.1038/s41587-019-0201-4" + licence: ['MIT'] + +input: + - gtf: + type: file + description: Reference gtf annotation file + pattern: "*.{gtf}" + +output: + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - splicesites: + type: file + description: Splices sites in gtf file + pattern: "*.{splice_sites.txt}" + +authors: + - "@ntoda03" diff --git a/modules/nf-core/software/rsem/preparereference/functions.nf b/modules/nf-core/software/rsem/preparereference/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/rsem/preparereference/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/rsem/preparereference/main.nf b/modules/nf-core/software/rsem/preparereference/main.nf new file mode 100644 index 00000000..a378eb6b --- /dev/null +++ b/modules/nf-core/software/rsem/preparereference/main.nf @@ -0,0 +1,67 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process RSEM_PREPAREREFERENCE { + tag "$fasta" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" + } else { + container "quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" + } + + input: + path fasta, stageAs: "rsem/*" + path gtf + + output: + path "rsem" , emit: index + path "rsem/*transcripts.fa", emit: transcript_fasta + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def args = options.args.tokenize() + if (args.contains('--star')) { + args.removeIf { it.contains('--star') } + def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' + """ + STAR \\ + --runMode genomeGenerate \\ + --genomeDir rsem/ \\ + --genomeFastaFiles $fasta \\ + --sjdbGTFfile $gtf \\ + --runThreadN $task.cpus \\ + $memory \\ + $options.args2 + + rsem-prepare-reference \\ + --gtf $gtf \\ + --num-threads $task.cpus \\ + ${args.join(' ')} \\ + $fasta \\ + rsem/genome + + rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt + """ + } else { + """ + rsem-prepare-reference \\ + --gtf $gtf \\ + --num-threads $task.cpus \\ + $options.args \\ + $fasta \\ + rsem/genome + + rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt + """ + } +} diff --git a/modules/nf-core/software/salmon/index/functions.nf b/modules/nf-core/software/salmon/index/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/salmon/index/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/salmon/index/main.nf b/modules/nf-core/software/salmon/index/main.nf new file mode 100644 index 00000000..17d5bc06 --- /dev/null +++ b/modules/nf-core/software/salmon/index/main.nf @@ -0,0 +1,51 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SALMON_INDEX { + tag "$transcript_fasta" + label "process_medium" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::salmon=1.4.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/salmon:1.4.0--hf69c8f4_0" + } else { + container "quay.io/biocontainers/salmon:1.4.0--hf69c8f4_0" + } + + input: + path genome_fasta + path transcript_fasta + + output: + path "salmon" , emit: index + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 > decoys.txt" + def gentrome = "gentrome.fa" + if (genome_fasta.endsWith('.gz')) { + get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 > decoys.txt" + gentrome = "gentrome.fa.gz" + } + """ + $get_decoy_ids + sed -i.bak -e 's/>//g' decoys.txt + cat $transcript_fasta $genome_fasta > $gentrome + + salmon \\ + index \\ + --threads $task.cpus \\ + -t $gentrome \\ + -d decoys.txt \\ + $options.args \\ + -i salmon + salmon --version | sed -e "s/salmon //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/salmon/index/meta.yml b/modules/nf-core/software/salmon/index/meta.yml new file mode 100644 index 00000000..652e2a77 --- /dev/null +++ b/modules/nf-core/software/salmon/index/meta.yml @@ -0,0 +1,35 @@ +name: salmon_index +description: Create index for salmon +keywords: + - index + - fasta + - genome + - reference +tools: + - salmon: + description: | + Salmon is a tool for wicked-fast transcript quantification from RNA-seq data + homepage: https://salmon.readthedocs.io/en/latest/salmon.html + manual: https://salmon.readthedocs.io/en/latest/salmon.html + doi: 10.1038/nmeth.4197 +input: + - genome_fasta: + type: file + description: Fasta file of the reference genome + - transcriptome_fasta: + type: file + description: Fasta file of the reference transcriptome + +output: + - index: + type: directory + description: Folder containing the star index files + pattern: "salmon" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@kevinmenden" + - "@drpatelh" diff --git a/modules/nf-core/software/samtools/flagstat/functions.nf b/modules/nf-core/software/samtools/flagstat/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/flagstat/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/flagstat/main.nf b/modules/nf-core/software/samtools/flagstat/main.nf new file mode 100644 index 00000000..7260f502 --- /dev/null +++ b/modules/nf-core/software/samtools/flagstat/main.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_FLAGSTAT { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + tuple val(meta), path(bam), path(bai) + + output: + tuple val(meta), path("*.flagstat"), emit: flagstat + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools flagstat $bam > ${bam}.flagstat + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/flagstat/meta.yml b/modules/nf-core/software/samtools/flagstat/meta.yml new file mode 100644 index 00000000..8414bf54 --- /dev/null +++ b/modules/nf-core/software/samtools/flagstat/meta.yml @@ -0,0 +1,48 @@ +name: samtools_flagstat +description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG type +keywords: + - stats + - mapping + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index for BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - flagstat: + type: file + description: File containing samtools flagstat output + pattern: "*.{flagstat}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" diff --git a/modules/nf-core/software/samtools/idxstats/functions.nf b/modules/nf-core/software/samtools/idxstats/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/idxstats/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/idxstats/main.nf b/modules/nf-core/software/samtools/idxstats/main.nf new file mode 100644 index 00000000..eb74a25e --- /dev/null +++ b/modules/nf-core/software/samtools/idxstats/main.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_IDXSTATS { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + tuple val(meta), path(bam), path(bai) + + output: + tuple val(meta), path("*.idxstats"), emit: idxstats + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools idxstats $bam > ${bam}.idxstats + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/idxstats/meta.yml b/modules/nf-core/software/samtools/idxstats/meta.yml new file mode 100644 index 00000000..530d0772 --- /dev/null +++ b/modules/nf-core/software/samtools/idxstats/meta.yml @@ -0,0 +1,49 @@ +name: samtools_idxstats +description: Reports alignment summary statistics for a BAM/CRAM/SAM file +keywords: + - stats + - mapping + - counts + - chromosome + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index for BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - idxstats: + type: file + description: File containing samtools idxstats output + pattern: "*.{idxstats}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" diff --git a/modules/nf-core/software/samtools/index/functions.nf b/modules/nf-core/software/samtools/index/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/index/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/index/main.nf b/modules/nf-core/software/samtools/index/main.nf new file mode 100644 index 00000000..2f323c81 --- /dev/null +++ b/modules/nf-core/software/samtools/index/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bai"), optional:true, emit: bai + tuple val(meta), path("*.csi"), optional:true, emit: csi + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools index $options.args $bam + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/index/meta.yml b/modules/nf-core/software/samtools/index/meta.yml new file mode 100644 index 00000000..5d076e3b --- /dev/null +++ b/modules/nf-core/software/samtools/index/meta.yml @@ -0,0 +1,47 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/software/samtools/sort/functions.nf b/modules/nf-core/software/samtools/sort/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/sort/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/sort/main.nf b/modules/nf-core/software/samtools/sort/main.nf new file mode 100644 index 00000000..ee09c656 --- /dev/null +++ b/modules/nf-core/software/samtools/sort/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + samtools sort $options.args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/sort/meta.yml b/modules/nf-core/software/samtools/sort/meta.yml new file mode 100644 index 00000000..704e8c1f --- /dev/null +++ b/modules/nf-core/software/samtools/sort/meta.yml @@ -0,0 +1,43 @@ +name: samtools_sort +description: Sort SAM/BAM/CRAM file +keywords: + - sort + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/software/samtools/stats/functions.nf b/modules/nf-core/software/samtools/stats/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/stats/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/stats/main.nf b/modules/nf-core/software/samtools/stats/main.nf new file mode 100644 index 00000000..c307f9e8 --- /dev/null +++ b/modules/nf-core/software/samtools/stats/main.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_STATS { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + tuple val(meta), path(bam), path(bai) + + output: + tuple val(meta), path("*.stats"), emit: stats + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools stats $bam > ${bam}.stats + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/stats/meta.yml b/modules/nf-core/software/samtools/stats/meta.yml new file mode 100644 index 00000000..b549ff5c --- /dev/null +++ b/modules/nf-core/software/samtools/stats/meta.yml @@ -0,0 +1,47 @@ +name: samtools_stats +description: Produces comprehensive statistics from SAM/BAM/CRAM file +keywords: + - statistics + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index for BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" diff --git a/modules/nf-core/software/star/align/functions.nf b/modules/nf-core/software/star/align/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/star/align/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/star/align/main.nf b/modules/nf-core/software/star/align/main.nf new file mode 100644 index 00000000..e85ddb79 --- /dev/null +++ b/modules/nf-core/software/star/align/main.nf @@ -0,0 +1,71 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process STAR_ALIGN { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + // Note: 2.7X indices incompatible with AWS iGenomes. + conda (params.enable_conda ? 'bioconda::star=2.6.1d' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/star:2.6.1d--0' + } else { + container 'quay.io/biocontainers/star:2.6.1d--0' + } + + input: + tuple val(meta), path(reads) + path index + path gtf + + output: + tuple val(meta), path('*d.out.bam') , emit: bam + tuple val(meta), path('*Log.final.out') , emit: log_final + tuple val(meta), path('*Log.out') , emit: log_out + tuple val(meta), path('*Log.progress.out'), emit: log_progress + path '*.version.txt' , emit: version + + tuple val(meta), path('*sortedByCoord.out.bam') , optional:true, emit: bam_sorted + tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript + tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted + tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq + tuple val(meta), path('*.tab') , optional:true, emit: tab + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" + def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" + def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' + def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' + """ + STAR \\ + --genomeDir $index \\ + --readFilesIn $reads \\ + --runThreadN $task.cpus \\ + --outFileNamePrefix $prefix. \\ + $out_sam_type \\ + $ignore_gtf \\ + $seq_center \\ + $options.args + + $mv_unsorted_bam + + if [ -f ${prefix}.Unmapped.out.mate1 ]; then + mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq + gzip ${prefix}.unmapped_1.fastq + fi + if [ -f ${prefix}.Unmapped.out.mate2 ]; then + mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq + gzip ${prefix}.unmapped_2.fastq + fi + + STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/star/align/meta.yml b/modules/nf-core/software/star/align/meta.yml new file mode 100644 index 00000000..01bc2ecf --- /dev/null +++ b/modules/nf-core/software/star/align/meta.yml @@ -0,0 +1,75 @@ +name: star_align +description: Align reads to a reference genome using STAR +keywords: + - align + - fasta + - genome + - reference +tools: + - star: + description: | + STAR is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: https://github.com/alexdobin/STAR + manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf + doi: 10.1093/bioinformatics/bts635 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - index: + type: directory + description: STAR genome index + pattern: "star" +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - log_final: + type: file + description: STAR final log file + pattern: "*Log.final.out" + - log_out: + type: file + description: STAR lot out file + pattern: "*Log.out" + - log_progress: + type: file + description: STAR log progress file + pattern: "*Log.progress.out" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - bam_sorted: + type: file + description: Sorted BAM file of read alignments (optional) + pattern: "*sortedByCoord.out.bam" + - bam_transcript: + type: file + description: Output BAM file of transcriptome alignment (optional) + pattern: "*toTranscriptome.out.bam" + - bam_unsorted: + type: file + description: Unsorted BAM file of read alignments (optional) + pattern: "*Aligned.unsort.out.bam" + - fastq: + type: file + description: Unmapped FastQ files (optional) + pattern: "*fastq.gz" + - tab: + type: file + description: STAR output tab file(s) (optional) + pattern: "*.tab" + +authors: + - "@kevinmenden" + - "@drpatelh" diff --git a/modules/nf-core/software/star/genomegenerate/functions.nf b/modules/nf-core/software/star/genomegenerate/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/star/genomegenerate/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/star/genomegenerate/main.nf b/modules/nf-core/software/star/genomegenerate/main.nf new file mode 100644 index 00000000..b440b415 --- /dev/null +++ b/modules/nf-core/software/star/genomegenerate/main.nf @@ -0,0 +1,67 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process STAR_GENOMEGENERATE { + tag "$fasta" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } + + // Note: 2.7X indices incompatible with AWS iGenomes. + conda (params.enable_conda ? "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0" + } else { + container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0" + } + + input: + path fasta + path gtf + + output: + path "star" , emit: index + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' + def args = options.args.tokenize() + if (args.contains('--genomeSAindexNbases')) { + """ + mkdir star + STAR \\ + --runMode genomeGenerate \\ + --genomeDir star/ \\ + --genomeFastaFiles $fasta \\ + --sjdbGTFfile $gtf \\ + --runThreadN $task.cpus \\ + $memory \\ + $options.args + + STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt + """ + } else { + """ + samtools faidx $fasta + NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai` + + mkdir star + STAR \\ + --runMode genomeGenerate \\ + --genomeDir star/ \\ + --genomeFastaFiles $fasta \\ + --sjdbGTFfile $gtf \\ + --runThreadN $task.cpus \\ + --genomeSAindexNbases \$NUM_BASES \\ + $memory \\ + $options.args + + STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt + """ + } +} diff --git a/modules/nf-core/software/star/genomegenerate/meta.yml b/modules/nf-core/software/star/genomegenerate/meta.yml new file mode 100644 index 00000000..aae0219b --- /dev/null +++ b/modules/nf-core/software/star/genomegenerate/meta.yml @@ -0,0 +1,36 @@ +name: star_genomegenerate +description: Create index for STAR +keywords: + - index + - fasta + - genome + - reference +tools: + - star: + description: | + STAR is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: https://github.com/alexdobin/STAR + manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf + doi: 10.1093/bioinformatics/bts635 +input: + - fasta: + type: file + description: Fasta file of the reference genome + - gtf: + type: file + description: GTF file of the reference genome + +output: + - index: + type: directory + description: Folder containing the star index files + pattern: "star" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + +authors: + - "@kevinmenden" + - "@drpatelh" diff --git a/modules/nf-core/software/untar/functions.nf b/modules/nf-core/software/untar/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/untar/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/untar/main.nf b/modules/nf-core/software/untar/main.nf new file mode 100644 index 00000000..fc6d7ec5 --- /dev/null +++ b/modules/nf-core/software/untar/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process UNTAR { + tag "$archive" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + path archive + + output: + path "$untar" , emit: untar + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + untar = archive.toString() - '.tar.gz' + """ + tar -xzvf $options.args $archive + echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/untar/meta.yml b/modules/nf-core/software/untar/meta.yml new file mode 100644 index 00000000..af4674f0 --- /dev/null +++ b/modules/nf-core/software/untar/meta.yml @@ -0,0 +1,27 @@ +name: untar +description: Extract files. +keywords: + - untar + - uncompress +tools: + - untar: + description: | + Extract tar.gz files. + documentation: https://www.gnu.org/software/tar/manual/ +input: + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" +output: + - untar: + type: file + description: + pattern: "*.*" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/nextflow.config b/nextflow.config index e268676e..59e794ae 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,192 +1,263 @@ /* - * ------------------------------------------------- - * nf-core/rnavar Nextflow config file - * ------------------------------------------------- - * Default config options for all environments. - */ +======================================================================================== + nf-core/rnaseq Nextflow config file +======================================================================================== + Default config options for all compute environments +---------------------------------------------------------------------------------------- +*/ // Global default params, used in configs params { - // Workflow flags - // TODO nf-core: Specify your pipeline's command line flags - genome = false - input = null - input_paths = null - single_end = false - outdir = './results' - publish_dir_mode = 'copy' - - // Boilerplate options - multiqc_config = false - email = false - email_on_fail = false - max_multiqc_email_size = 25.MB - plaintext_email = false - monochrome_logs = false - help = false - igenomes_base = 's3://ngi-igenomes/igenomes' - tracedir = "${params.outdir}/pipeline_info" - igenomes_ignore = false - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = false - config_profile_name = null - config_profile_description = false - config_profile_contact = false - config_profile_url = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes,input_paths' - - // Defaults only, expecting to be overwritten - max_memory = 128.GB - max_cpus = 16 - max_time = 240.h + // Input options + input = null + public_data_ids = null + skip_sra_fastq_download = false -} + // References + genome = null + transcript_fasta = null + additional_fasta = null + splicesites = null + gtf_extra_attributes = 'gene_name' + gtf_group_features = 'gene_id' + featurecounts_feature_type = 'exon' + featurecounts_group_type = 'gene_biotype' + gencode = false + save_reference = false + + // UMI handling + with_umi = false + umitools_extract_method = 'string' + umitools_bc_pattern = null + save_umi_intermeds = false + + // Trimming + clip_r1 = null + clip_r2 = null + three_prime_clip_r1 = null + three_prime_clip_r2 = null + trim_nextseq = null + save_trimmed = false + skip_trimming = false + + // Ribosomal RNA removal + remove_ribo_rna = false + save_non_ribo_reads = false + ribo_database_manifest = "${projectDir}/assets/rrna-db-defaults.txt" + + // Alignment + aligner = 'star_salmon' + pseudo_aligner = null + seq_center = null + bam_csi_index = false + star_ignore_sjdbgtf = false + hisat2_build_memory = '200.GB' // Amount of memory required to build HISAT2 index with splice sites + stringtie_ignore_gtf = false + min_mapped_reads = 5 + save_merged_fastq = false + save_unaligned = false + save_align_intermeds = false + skip_markduplicates = false + skip_alignment = false + + // QC + skip_qc = false + skip_bigwig = false + skip_stringtie = false + skip_fastqc = false + skip_preseq = false + skip_dupradar = false + skip_qualimap = false + skip_rseqc = false + skip_biotype_qc = false + skip_deseq2_qc = false + skip_multiqc = false + deseq2_vst = false + rseqc_modules = 'bam_stat,inner_distance,infer_experiment,junction_annotation,junction_saturation,read_distribution,read_duplication' + + // Boilerplate options + outdir = './results' + publish_dir_mode = 'copy' + multiqc_config = null + multiqc_title = null + email = null + email_on_fail = null + max_multiqc_email_size = '25.MB' + plaintext_email = false + monochrome_logs = false + help = false + igenomes_base = 's3://ngi-igenomes/igenomes/' + tracedir = "${params.outdir}/pipeline_info" + igenomes_ignore = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,modules' + enable_conda = false + singularity_pull_docker_container = false -// Container slug. Stable releases should specify release tag! -// Developmental code should specify :dev -process.container = 'nfcore/rnavar:dev' + // Config options + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + hostnames = [:] + config_profile_name = null + config_profile_description = null + config_profile_contact = null + config_profile_url = null + + // Defaults only, expecting to be overwritten + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' +} // Load base.config by default for all pipelines includeConfig 'conf/base.config' +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + // Load nf-core custom profiles from different Institutions try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" + includeConfig "${params.custom_config_base}/nfcore_custom.config" } catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -profiles { - conda { - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - process.conda = "$projectDir/environment.yml" - } - debug { process.beforeScript = 'echo $HOSTNAME' } - docker { - docker.enabled = true - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - // Avoid this error: - // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. - // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 - // once this is established and works well, nextflow might implement this behavior as new default. - docker.runOptions = '-u \$(id -u):\$(id -g)' - } - singularity { - docker.enabled = false - singularity.enabled = true - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - singularity.autoMounts = true - } - podman { - singularity.enabled = false - docker.enabled = false - podman.enabled = true - shifter.enabled = false - charliecloud.enabled = false - } - shifter { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = true - charliecloud.enabled = false - } - charliecloud { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = true - } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } +// Load nf-core/rnaseq custom config +try { + includeConfig "${params.custom_config_base}/pipeline/rnaseq.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/rnaseq profiles: ${params.custom_config_base}/pipeline/rnaseq.config") } // Load igenomes.config if required if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} + +profiles { + debug { process.beforeScript = 'echo $HOSTNAME' } + conda { + params.enable_conda = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + docker { + docker.enabled = true + // Avoid this error: + // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. + // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 + // once this is established and works well, nextflow might implement this behavior as new default. + docker.runOptions = '-u \$(id -u):\$(id -g)' + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + singularity { + singularity.enabled = true + singularity.autoMounts = true + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + podman { + podman.enabled = true + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + shifter { + shifter.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + } + charliecloud { + charliecloud.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + } + test { includeConfig 'conf/test.config' } + test_sra { includeConfig 'conf/test_sra.config' } + test_full { includeConfig 'conf/test_full.config' } } // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { - PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" } -// Capture exit codes from upstream processes when piping -process.shell = ['/bin/bash', '-euo', 'pipefail'] - def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { - enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" } report { - enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" } trace { - enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" + enabled = true + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" } dag { - enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" + enabled = true + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" } manifest { - name = 'nf-core/rnavar' - author = 'Maxime U Garcia' - homePage = 'https://github.com/nf-core/rnavar' - description = 'gatk4 rna variant calling' - mainScript = 'main.nf' - nextflowVersion = '>=20.04.0' - version = '1.0dev' + name = 'nf-core/rnaseq' + author = 'Phil Ewels, Rickard Hammarén' + homePage = 'https://github.com/nf-core/rnaseq' + description = 'Nextflow RNA-Seq analysis pipeline, part of the nf-core community.' + mainScript = 'main.nf' + nextflowVersion = '!>=21.04.0' + version = '3.1' } // Function to ensure that resource requirements don't go beyond // a maximum limit def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } } - } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 1ab98172..28ed6abb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,8 +1,8 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/nextflow_schema.json", - "title": "nf-core/rnavar pipeline parameters", - "description": "gatk4 rna variant calling", + "$id": "https://raw.githubusercontent.com/nf-core/rnaseq/master/nextflow_schema.json", + "title": "nf-core/rnaseq pipeline parameters", + "description": "Nextflow RNA-Seq analysis pipeline, part of the nf-core community.", "type": "object", "definitions": { "input_output_options": { @@ -10,57 +10,259 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input" - ], "properties": { "input": { "type": "string", - "fa_icon": "fas fa-dna", - "description": "Input FastQ files.", - "help_text": "Use this to specify the location of your input FastQ files. For example:\n\n```bash\n--input 'path/to/data/sample_*_{1,2}.fastq'\n```\n\nPlease note the following requirements:\n\n1. The path must be enclosed in quotes\n2. The path must have at least one `*` wildcard character\n3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs.\n\nIf left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz`" - }, - "single_end": { - "type": "boolean", - "description": "Specifies that the input is single-end reads.", - "fa_icon": "fas fa-align-center", - "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example:\n\n```bash\n--single_end --input '*.fastq'\n```\n\nIt is not possible to run a mixture of single-end and paired-end files in one run." + "format": "file-path", + "mimetype": "text/csv", + "pattern": "\\.csv$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/rnaseq/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", - "description": "The output directory where the results will be saved.", + "description": "Path to the output directory where the results will be saved.", "default": "./results", "fa_icon": "fas fa-folder-open" }, + "public_data_ids": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.txt$", + "schema": "assets/schema_public_data_ids.json", + "fa_icon": "fas fa-database", + "description": "File containing SRA/ENA/GEO identifiers one per line in order to download their associated FastQ files." + }, "email": { "type": "string", "description": "Email address for completion summary.", "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + }, + "skip_sra_fastq_download": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Only download metadata for public data database ids and don't download the FastQ files." + }, + "save_merged_fastq": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save FastQ files after merging re-sequenced libraries in the results directory." } } }, + "umi_options": { + "title": "UMI options", + "type": "object", + "description": "Options for processing reads with unique molecular identifiers", + "default": "", + "properties": { + "with_umi": { + "type": "boolean", + "fa_icon": "fas fa-barcode", + "description": "Enable UMI-based read deduplication." + }, + "umitools_extract_method": { + "type": "string", + "default": "string", + "fa_icon": "fas fa-barcode", + "description": "UMI pattern to use. Can be either 'string' (default) or 'regex'.", + "help_text": "More details can be found in the [UMI-tools documentation](https://umi-tools.readthedocs.io/en/latest/reference/extract.html#extract-method).\n" + }, + "umitools_bc_pattern": { + "type": "string", + "fa_icon": "fas fa-barcode", + "help_text": "More details can be found in the [UMI-tools documentation](https://umi-tools.readthedocs.io/en/latest/reference/extract.html#extract-method).", + "description": "The UMI barcode pattern to use e.g. 'NNNNNN' indicates that the first 6 nucleotides of the read are from the UMI." + }, + "save_umi_intermeds": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "If this option is specified, intermediate FastQ and BAM files produced by UMI-tools are also saved in the results directory." + } + }, + "fa_icon": "fas fa-barcode" + }, + "read_filtering_options": { + "title": "Read filtering options", + "type": "object", + "description": "Options for filtering reads prior to alignment", + "default": "", + "properties": { + "remove_ribo_rna": { + "type": "boolean", + "fa_icon": "fas fa-trash-alt", + "description": "Enable the removal of reads derived from ribosomal RNA using SortMeRNA.", + "help_text": "Any patterns found in the sequences defined by the '--ribo_database_manifest' parameter will be used." + }, + "ribo_database_manifest": { + "type": "string", + "default": "${projectDir}/assets/rrna-db-defaults.txt", + "fa_icon": "fas fa-database", + "description": "Text file containing paths to fasta files (one per line) that will be used to create the database for SortMeRNA.", + "help_text": "By default, [rRNA databases](https://github.com/biocore/sortmerna/tree/master/data/rRNA_databases) defined in the SortMeRNA GitHub repo are used. You can see an example in the pipeline Github repository in `assets/rrna-default-dbs.txt`.\nPlease note that commercial/non-academic entities require [`licensing for SILVA`](https://www.arb-silva.de/silva-license-information) for these default databases." + }, + "save_non_ribo_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "If this option is specified, intermediate FastQ files containing non-rRNA reads will be saved in the results directory." + } + }, + "fa_icon": "fas fa-trash-alt" + }, "reference_genome_options": { "title": "Reference genome options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for the reference genome indices used to align reads.", + "description": "Reference genome related files and options required for the workflow.", "properties": { "genome": { "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." }, "fasta": { "type": "string", - "fa_icon": "fas fa-font", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", - "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "gtf": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.gtf(\\.gz)?$", + "description": "Path to GTF annotation file.", + "fa_icon": "fas fa-code-branch", + "help_text": "This parameter is *mandatory* if `--genome` is not specified." + }, + "gff": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.gff(\\.gz)?$", + "fa_icon": "fas fa-code-branch", + "description": "Path to GFF3 annotation file.", + "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." + }, + "gene_bed": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.bed(\\.gz)?$", + "fa_icon": "fas fa-procedures", + "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." + }, + "transcript_fasta": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.fn?a(sta)?(\\.gz)?$", + "fa_icon": "far fa-file-code", + "description": "Path to FASTA transcriptome file." + }, + "additional_fasta": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.fn?a(sta)?(\\.gz)?$", + "fa_icon": "far fa-file-code", + "description": "FASTA file to concatenate to genome FASTA file e.g. containing spike-in sequences.", + "help_text": "If provided, the sequences in this file will get concatenated to the existing genome FASTA file, a GTF file will be automatically created using the entire sequence as the gene, transcript, and exon features, and any alignment index will get created from the combined FASTA and GTF. It is recommended to save the reference with `--save_reference` to re-use the index for future runs so you do not need to create it again." + }, + "splicesites": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "fa_icon": "fas fa-hand-scissors", + "description": "Splice sites file required for HISAT2." + }, + "star_index": { + "type": "string", + "format": "path", + "fa_icon": "fas fa-bezier-curve", + "description": "Path to directory or tar.gz archive for pre-built STAR index." + }, + "hisat2_index": { + "type": "string", + "format": "path", + "fa_icon": "fas fa-bezier-curve", + "description": "Path to directory or tar.gz archive for pre-built HISAT2 index." + }, + "rsem_index": { + "type": "string", + "format": "path", + "fa_icon": "fas fa-bezier-curve", + "description": "Path to directory or tar.gz archive for pre-built RSEM index." + }, + "salmon_index": { + "type": "string", + "format": "path", + "fa_icon": "fas fa-bezier-curve", + "description": "Path to directory or tar.gz archive for pre-built Salmon index." + }, + "hisat2_build_memory": { + "type": "string", + "default": "200.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "description": "Minimum memory required to use splice sites and exons in the HiSAT2 index build process.", + "help_text": "HiSAT2 requires a huge amount of RAM to build a genome index for larger genomes, if including splice sites and exons e.g. the human genome might typically require 200GB. If you specify less than this threshold for the `HISAT2_BUILD` process then the splice sites and exons will be ignored, meaning that the process will require a lot less memory. If you are working with a small genome, set this parameter to a lower value to reduce the threshold for skipping this check. If using a larger genome, consider supplying more memory to the `HISAT2_BUILD` process." + }, + "gencode": { + "type": "boolean", + "fa_icon": "fas fa-code-branch", + "description": "Specify if your GTF annotation is in GENCODE format.", + "help_text": "If your GTF file is in GENCODE format and you would like to run Salmon i.e. `--pseudo_aligner salmon`, you will need to provide this parameter in order to build the Salmon index appropriately." + }, + "gtf_extra_attributes": { + "type": "string", + "default": "gene_name", + "fa_icon": "fas fa-plus-square", + "description": "By default, the pipeline uses the `gene_name` field to obtain additional gene identifiers from the input GTF file when running Salmon.", + "help_text": "This behaviour can be modified by specifying `--gtf_extra_attributes` when running the pipeline. Note that you can also specify more than one desired value, separated by a comma e.g. `--gtf_extra_attributes gene_id,...`.\n" + }, + "gtf_group_features": { + "type": "string", + "default": "gene_id", + "description": "Define the attribute type used to group features in the GTF file when running Salmon.", + "fa_icon": "fas fa-layer-group" + }, + "featurecounts_group_type": { + "type": "string", + "default": "gene_biotype", + "fa_icon": "fas fa-layer-group", + "description": "The attribute type used to group feature types in the GTF file when generating the biotype plot with featureCounts." + }, + "featurecounts_feature_type": { + "type": "string", + "default": "exon", + "description": "By default, the pipeline assigns reads based on the 'exon' attribute within the GTF file.", + "fa_icon": "fas fa-indent", + "help_text": "The feature type used from the GTF file when generating the biotype plot with featureCounts." + }, + "save_reference": { + "type": "boolean", + "description": "If generated by the pipeline save the BWA index in the results directory.", + "help_text": "If the BWA index is generated by the pipeline use this parameter to save it to your results folder. These can then be used for future pipeline runs, reducing processing times.", + "fa_icon": "fas fa-save" }, "igenomes_base": { "type": "string", + "format": "directory-path", "description": "Directory / URL base for iGenomes references.", "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", @@ -75,126 +277,200 @@ } } }, - "generic_options": { - "title": "Generic options", + "read_trimming_options": { + "title": "Read trimming options", "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "fa_icon": "fas fa-cut", + "description": "Options to adjust read trimming criteria.", "properties": { - "help": { + "clip_r1": { + "type": "integer", + "description": "Instructs Trim Galore to remove bp from the 5' end of read 1 (or single-end reads).", + "fa_icon": "fas fa-cut" + }, + "clip_r2": { + "type": "integer", + "description": "Instructs Trim Galore to remove bp from the 5' end of read 2 (paired-end reads only).", + "fa_icon": "fas fa-cut" + }, + "three_prime_clip_r1": { + "type": "integer", + "description": "Instructs Trim Galore to remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed.", + "fa_icon": "fas fa-cut" + }, + "three_prime_clip_r2": { + "type": "integer", + "description": "Instructs Trim Galore to remove bp from the 3' end of read 2 AFTER adapter/quality trimming has been performed.", + "fa_icon": "fas fa-cut" + }, + "trim_nextseq": { + "type": "integer", + "description": "Instructs Trim Galore to apply the --nextseq=X option, to trim based on quality after removing poly-G tails.", + "help_text": "This enables the option Cutadapt `--nextseq-trim=3'CUTOFF` option via Trim Galore, which will set a quality cutoff (that is normally given with -q instead), but qualities of G bases are ignored. This trimming is in common for the NextSeq- and NovaSeq-platforms, where basecalls without any signal are called as high-quality G bases.", + "fa_icon": "fas fa-cut" + }, + "skip_trimming": { "type": "boolean", - "description": "Display help text.", - "hidden": true, - "fa_icon": "fas fa-question-circle" + "description": "Skip the adapter trimming step.", + "help_text": "Use this if your input FastQ files have already been trimmed outside of the workflow or if you're very confident that there is no adapter contamination in your data.", + "fa_icon": "fas fa-fast-forward" }, - "publish_dir_mode": { + "save_trimmed": { + "type": "boolean", + "description": "Save the trimmed FastQ files in the results directory.", + "help_text": "By default, trimmed FastQ files will not be saved to the results directory. Specify this flag (or set to true in your config file) to copy these files to the results directory when complete.", + "fa_icon": "fas fa-save" + } + } + }, + "alignment_options": { + "title": "Alignment options", + "type": "object", + "fa_icon": "fas fa-map-signs", + "description": "Options to adjust parameters and filtering criteria for read alignments.", + "properties": { + "aligner": { "type": "string", - "default": "copy", - "hidden": true, - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", + "default": "star_salmon", + "description": "Specifies the alignment algorithm to use - available options are 'star_salmon', 'star_rsem' and 'hisat2'.", + "fa_icon": "fas fa-map-signs", "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" + "star_salmon", + "star_rsem", + "hisat2" ] }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "email_on_fail": { + "pseudo_aligner": { "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "hidden": true, - "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + "description": "Specifies the pseudo aligner to use - available options are 'salmon'. Runs in addition to '--aligner'.", + "fa_icon": "fas fa-hamburger", + "enum": [ + "salmon" + ] }, - "plaintext_email": { + "bam_csi_index": { "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true, - "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + "description": "Create a CSI index for BAM files instead of the traditional BAI index. This will be required for genomes with larger chromosome sizes.", + "fa_icon": "fas fa-sort-alpha-down" }, - "max_multiqc_email_size": { + "star_ignore_sjdbgtf": { + "type": "boolean", + "fa_icon": "fas fa-ban", + "description": "When using pre-built STAR indices do not re-extract and use splice junctions from the GTF file." + }, + "min_mapped_reads": { + "type": "integer", + "default": 5, + "fa_icon": "fas fa-percentage", + "description": "Minimum percentage of uniquely mapped reads below which samples are removed from further processing.", + "help_text": "Some downstream steps in the pipeline will fail if this threshold is too low." + }, + "seq_center": { "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true, - "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." + "description": "Sequencing center information to be added to read group of BAM files.", + "fa_icon": "fas fa-synagogue" }, - "monochrome_logs": { + "stringtie_ignore_gtf": { "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true, - "help_text": "Set to disable colourful command line output and live life in monochrome." + "description": "Perform reference-guided de novo assembly of transcripts using StringTie i.e. dont restrict to those in GTF file.", + "fa_icon": "fas fa-ban" }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true + "save_unaligned": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Where possible, save unaligned reads from either STAR, HISAT2 or Salmon to the results directory.", + "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true + "save_align_intermeds": { + "type": "boolean", + "description": "Save the intermediate BAM files from the alignment step.", + "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files.", + "fa_icon": "fas fa-save" }, - "show_hidden_params": { + "skip_markduplicates": { "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + "fa_icon": "fas fa-fast-forward", + "description": "Skip picard MarkDuplicates step." + }, + "skip_alignment": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip all of the alignment-based processes within the pipeline." } } }, - "max_job_request_options": { - "title": "Max job request options", + "process_skipping_options": { + "title": "Process skipping options", "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "fa_icon": "fas fa-fast-forward", + "description": "Options to skip various steps within the workflow.", "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { + "rseqc_modules": { "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + "default": "bam_stat,inner_distance,infer_experiment,junction_annotation,junction_saturation,read_distribution,read_duplication", + "fa_icon": "fas fa-chart-pie", + "description": "Specify the RSeQC modules to run." }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + "deseq2_vst": { + "type": "boolean", + "description": "Use vst transformation instead of rlog with DESeq2.", + "help_text": "See [DESeq2 docs](http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#data-transformations-and-visualization).", + "fa_icon": "fas fa-dolly" + }, + "skip_bigwig": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip bigWig file creation." + }, + "skip_stringtie": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip StringTie." + }, + "skip_fastqc": { + "type": "boolean", + "description": "Skip FastQC.", + "fa_icon": "fas fa-fast-forward" + }, + "skip_preseq": { + "type": "boolean", + "description": "Skip Preseq.", + "fa_icon": "fas fa-fast-forward" + }, + "skip_dupradar": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip dupRadar." + }, + "skip_qualimap": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip Qualimap." + }, + "skip_rseqc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip RSeQC." + }, + "skip_biotype_qc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip additional featureCounts process for biotype QC." + }, + "skip_deseq2_qc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip DESeq2 PCA and heatmap plotting." + }, + "skip_multiqc": { + "type": "boolean", + "description": "Skip MultiQC.", + "fa_icon": "fas fa-fast-forward" + }, + "skip_qc": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip all QC steps except for MultiQC." } } }, @@ -210,15 +486,14 @@ "description": "Git commit id for Institutional configs.", "default": "master", "hidden": true, - "fa_icon": "fas fa-users-cog", - "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```" + "fa_icon": "fas fa-users-cog" }, "custom_config_base": { "type": "string", "description": "Base directory for Institutional configs.", "default": "https://raw.githubusercontent.com/nf-core/configs/master", "hidden": true, - "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.", + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", "fa_icon": "fas fa-users-cog" }, "hostnames": { @@ -252,23 +527,171 @@ "fa_icon": "fas fa-users-cog" } } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", + "hidden": true, + "fa_icon": "fas fa-toolbox", + "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." + } + } } }, "allOf": [ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/umi_options" + }, + { + "$ref": "#/definitions/read_filtering_options" + }, { "$ref": "#/definitions/reference_genome_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/definitions/read_trimming_options" }, { - "$ref": "#/definitions/max_job_request_options" + "$ref": "#/definitions/alignment_options" + }, + { + "$ref": "#/definitions/process_skipping_options" }, { "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" } ] } diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf new file mode 100644 index 00000000..f2618388 --- /dev/null +++ b/subworkflows/local/input_check.nf @@ -0,0 +1,43 @@ +// +// Check input samplesheet and get read channels +// + +params.options = [:] + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channels(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def create_fastq_channels(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + meta.strandedness = row.strandedness + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf new file mode 100644 index 00000000..60064264 --- /dev/null +++ b/subworkflows/local/prepare_genome.nf @@ -0,0 +1,215 @@ +// +// Uncompress and prepare reference genome files +// + +params.genome_options = [:] +params.index_options = [:] +params.gffread_options = [:] +params.star_index_options = [:] +params.rsem_index_options = [:] +params.hisat2_index_options = [:] +params.salmon_index_options = [:] + +include { + GUNZIP as GUNZIP_FASTA + GUNZIP as GUNZIP_GTF + GUNZIP as GUNZIP_GFF + GUNZIP as GUNZIP_GENE_BED + GUNZIP as GUNZIP_TRANSCRIPT_FASTA + GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/software/gunzip/main' addParams( options: params.genome_options ) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.star_index_options ) +include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.index_options ) +include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.hisat2_index_options ) +include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.index_options ) +include { GFFREAD } from '../../modules/nf-core/software/gffread/main' addParams( options: params.gffread_options ) +include { STAR_GENOMEGENERATE } from '../../modules/nf-core/software/star/genomegenerate/main' addParams( options: params.star_index_options ) +include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/software/hisat2/extractsplicesites/main' addParams( options: params.hisat2_index_options ) +include { HISAT2_BUILD } from '../../modules/nf-core/software/hisat2/build/main' addParams( options: params.hisat2_index_options ) +include { SALMON_INDEX } from '../../modules/nf-core/software/salmon/index/main' addParams( options: params.salmon_index_options ) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/software/rsem/preparereference/main' addParams( options: params.rsem_index_options ) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/software/rsem/preparereference/main' addParams( options: params.genome_options ) + +include { GTF2BED } from '../../modules/local/gtf2bed' addParams( options: params.genome_options ) +include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams( options: params.genome_options ) +include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams( options: params.genome_options ) +include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams( options: params.genome_options ) + +workflow PREPARE_GENOME { + take: + prepare_tool_indices // list : tools to prepare indices for + biotype // string: if additional fasta file is provided + // biotype value to use when appending entries to GTF file + + main: + + // + // Uncompress genome fasta file if required + // + if (params.fasta.endsWith('.gz')) { + ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzip + } else { + ch_fasta = file(params.fasta) + } + + // + // Uncompress GTF annotation file or create from GFF3 if required + // + ch_gffread_version = Channel.empty() + if (params.gtf) { + if (params.gtf.endsWith('.gz')) { + ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip + } else { + ch_gtf = file(params.gtf) + } + } else if (params.gff) { + if (params.gff.endsWith('.gz')) { + ch_gff = GUNZIP_GFF ( params.gff ).gunzip + } else { + ch_gff = file(params.gff) + } + ch_gtf = GFFREAD ( ch_gff ).gtf + ch_gffread_version = GFFREAD.out.version + } + + // + // Uncompress additional fasta file and concatenate with reference fasta and gtf files + // + if (params.additional_fasta) { + if (params.additional_fasta.endsWith('.gz')) { + ch_add_fasta = GUNZIP_ADDITIONAL_FASTA ( params.additional_fasta ).gunzip + } else { + ch_add_fasta = file(params.additional_fasta) + } + CAT_ADDITIONAL_FASTA ( ch_fasta, ch_gtf, ch_add_fasta, biotype ) + ch_fasta = CAT_ADDITIONAL_FASTA.out.fasta + ch_gtf = CAT_ADDITIONAL_FASTA.out.gtf + } + + // + // Uncompress gene BED annotation file or create from GTF if required + // + if (params.gene_bed) { + if (params.gene_bed.endsWith('.gz')) { + ch_gene_bed = GUNZIP_GENE_BED ( params.gene_bed ).gunzip + } else { + ch_gene_bed = file(params.gene_bed) + } + } else { + ch_gene_bed = GTF2BED ( ch_gtf ) + } + + // + // Uncompress transcript fasta file / create if required + // + ch_rsem_version = Channel.empty() + if (params.transcript_fasta) { + if (params.transcript_fasta.endsWith('.gz')) { + ch_transcript_fasta = GUNZIP_TRANSCRIPT_FASTA ( params.transcript_fasta ).gunzip + } else { + ch_transcript_fasta = file(params.transcript_fasta) + } + } else { + ch_transcript_fasta = RSEM_PREPAREREFERENCE_TRANSCRIPTS ( ch_fasta, GTF_GENE_FILTER ( ch_fasta, ch_gtf ) ).transcript_fasta + ch_rsem_version = RSEM_PREPAREREFERENCE_TRANSCRIPTS.out.version + } + + // + // Create chromosome sizes file + // + ch_chrom_sizes = GET_CHROM_SIZES ( ch_fasta ).sizes + + // + // Uncompress STAR index or generate from scratch if required + // + ch_star_index = Channel.empty() + ch_star_version = Channel.empty() + if ('star_salmon' in prepare_tool_indices) { + if (params.star_index) { + if (params.star_index.endsWith('.tar.gz')) { + ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar + } else { + ch_star_index = file(params.star_index) + } + } else { + ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index + ch_star_version = STAR_GENOMEGENERATE.out.version + } + } + + // + // Uncompress RSEM index or generate from scratch if required + // + ch_rsem_index = Channel.empty() + if ('star_rsem' in prepare_tool_indices) { + if (params.rsem_index) { + if (params.rsem_index.endsWith('.tar.gz')) { + ch_rsem_index = UNTAR_RSEM_INDEX ( params.rsem_index ).untar + } else { + ch_rsem_index = file(params.rsem_index) + } + } else { + ch_rsem_index = RSEM_PREPAREREFERENCE ( ch_fasta, ch_gtf ).index + ch_rsem_version = RSEM_PREPAREREFERENCE.out.version + } + } + + // + // Uncompress HISAT2 index or generate from scratch if required + // + ch_splicesites = Channel.empty() + ch_hisat2_index = Channel.empty() + ch_hisat2_version = Channel.empty() + if ('hisat2' in prepare_tool_indices) { + if (!params.splicesites) { + ch_splicesites = HISAT2_EXTRACTSPLICESITES ( ch_gtf ).txt + ch_hisat2_version = HISAT2_EXTRACTSPLICESITES.out.version + } else { + ch_splicesites = file(params.splicesites) + } + if (params.hisat2_index) { + if (params.hisat2_index.endsWith('.tar.gz')) { + ch_hisat2_index = UNTAR_HISAT2_INDEX ( params.hisat2_index ).untar + } else { + ch_hisat2_index = file(params.hisat2_index) + } + } else { + ch_hisat2_index = HISAT2_BUILD ( ch_fasta, ch_gtf, ch_splicesites ).index + ch_hisat2_version = HISAT2_BUILD.out.version + } + } + + // + // Uncompress Salmon index or generate from scratch if required + // + ch_salmon_index = Channel.empty() + ch_salmon_version = Channel.empty() + if ('salmon' in prepare_tool_indices) { + if (params.salmon_index) { + if (params.salmon_index.endsWith('.tar.gz')) { + ch_salmon_index = UNTAR_SALMON_INDEX ( params.salmon_index ).untar + } else { + ch_salmon_index = file(params.salmon_index) + } + } else { + ch_salmon_index = SALMON_INDEX ( ch_fasta, ch_transcript_fasta ).index + ch_salmon_version = SALMON_INDEX.out.version + } + } + + emit: + fasta = ch_fasta // path: genome.fasta + gtf = ch_gtf // path: genome.gtf + gene_bed = ch_gene_bed // path: gene.bed + transcript_fasta = ch_transcript_fasta // path: transcript.fasta + chrom_sizes = ch_chrom_sizes // path: genome.sizes + splicesites = ch_splicesites // path: genome.splicesites.txt + star_index = ch_star_index // path: star/index/ + rsem_index = ch_rsem_index // path: rsem/index/ + hisat2_index = ch_hisat2_index // path: hisat2/index/ + salmon_index = ch_salmon_index // path: salmon/index/ + star_version = ch_star_version // path: *.version.txt + rsem_version = ch_rsem_version // path: *.version.txt + hisat2_version = ch_hisat2_version // path: *.version.txt + salmon_version = ch_salmon_version // path: *.version.txt + gffread_version = ch_gffread_version // path: *.version.txt +} diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf new file mode 100644 index 00000000..7d228e1a --- /dev/null +++ b/subworkflows/nf-core/align_star.nf @@ -0,0 +1,49 @@ +// +// Alignment with STAR +// + +params.align_options = [:] +params.samtools_sort_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { STAR_ALIGN } from '../../modules/nf-core/software/star/align/main' addParams( options: params.align_options ) +include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) + +workflow ALIGN_STAR { + take: + reads // channel: [ val(meta), [ reads ] ] + index // channel: /path/to/star/index/ + gtf // channel: /path/to/genome.gtf + + main: + + // + // Map reads with STAR + // + STAR_ALIGN ( reads, index, gtf ) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) + + emit: + orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] + log_final = STAR_ALIGN.out.log_final // channel: [ val(meta), log_final ] + log_out = STAR_ALIGN.out.log_out // channel: [ val(meta), log_out ] + log_progress = STAR_ALIGN.out.log_progress // channel: [ val(meta), log_progress ] + bam_sorted = STAR_ALIGN.out.bam_sorted // channel: [ val(meta), bam_sorted ] + bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] + fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] + tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] + star_version = STAR_ALIGN.out.version // path: *.version.txt + + bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] + csi = BAM_SORT_SAMTOOLS.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = BAM_SORT_SAMTOOLS.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf new file mode 100644 index 00000000..4ae896dc --- /dev/null +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -0,0 +1,45 @@ +// +// Sort, index BAM file and run samtools stats, flagstat and idxstats +// + +params.sort_options = [:] +params.index_options = [:] +params.stats_options = [:] + +include { SAMTOOLS_SORT } from '../../modules/nf-core/software/samtools/sort/main' addParams( options: params.sort_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) + +workflow BAM_SORT_SAMTOOLS { + take: + ch_bam // channel: [ val(meta), [ bam ] ] + + main: + SAMTOOLS_SORT ( ch_bam ) + SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + + SAMTOOLS_SORT.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai ) + + emit: + bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + version = SAMTOOLS_SORT.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf new file mode 100644 index 00000000..eca9b37a --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -0,0 +1,25 @@ +// +// Run SAMtools stats, flagstat and idxstats +// + +params.options = [:] + +include { SAMTOOLS_STATS } from '../../modules/nf-core/software/samtools/stats/main' addParams( options: params.options ) +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/software/samtools/idxstats/main' addParams( options: params.options ) +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/software/samtools/flagstat/main' addParams( options: params.options ) + +workflow BAM_STATS_SAMTOOLS { + take: + ch_bam_bai // channel: [ val(meta), [ bam ], [bai/csi] ] + + main: + SAMTOOLS_STATS ( ch_bam_bai ) + SAMTOOLS_FLAGSTAT ( ch_bam_bai ) + SAMTOOLS_IDXSTATS ( ch_bam_bai ) + + emit: + stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + version = SAMTOOLS_STATS.out.version // path: *.version.txt +} diff --git a/workflows/rnaseq.nf b/workflows/rnaseq.nf new file mode 100644 index 00000000..c4f2e7c5 --- /dev/null +++ b/workflows/rnaseq.nf @@ -0,0 +1,145 @@ +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. + + +def valid_params = [ + aligners : ['star_salmon', 'star_rsem', 'hisat2'], + pseudoaligners : ['salmon'], + rseqc_modules : ['bam_stat', 'inner_distance', 'infer_experiment', 'junction_annotation', 'junction_saturation', 'read_distribution', 'read_duplication'] +] + +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) + +// Validate input parameters +WorkflowRnaseq.initialise(params, log, valid_params) + +// Check input path parameters to see if they exist +checkPathParamList = [ + params.input, + params.fasta, + params.gtf, params.gff, params.gene_bed, + params.star_index +] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +// Check alignment parameters +def prepareToolIndices = [] +if (!params.skip_alignment) { prepareToolIndices << params.aligner } + +def modules = params.modules.clone() + +def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] +def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] + + +def star_genomegenerate_options = modules['star_genomegenerate'] +if (!params.save_reference) { star_genomegenerate_options['publish_files'] = false } + +def samtools_sort_genome_options = modules['samtools_sort_genome'] +def samtools_index_genome_options = modules['samtools_index_genome'] +samtools_index_genome_options.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +if (['star_salmon','hisat2'].contains(params.aligner)) { + if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { + samtools_sort_genome_options.publish_files.put('bam','') + samtools_index_genome_options.publish_files.put('bai','') + samtools_index_genome_options.publish_files.put('csi','') + } +} else { + if (params.save_align_intermeds || params.skip_markduplicates) { + samtools_sort_genome_options.publish_files.put('bam','') + samtools_index_genome_options.publish_files.put('bai','') + samtools_index_genome_options.publish_files.put('csi','') + } +} + +def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type + +def star_align_options = modules['star_align'] +star_align_options.args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +if (params.save_align_intermeds) { star_align_options.publish_files.put('bam','') } +if (params.save_unaligned) { star_align_options.publish_files.put('fastq.gz','unmapped') } + +def cat_fastq_options = modules['cat_fastq'] +if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } + +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) + +workflow RNASEQ_VAR { + + PREPARE_GENOME ( + prepareToolIndices, + biotype + ) + ch_software_versions = Channel.empty() + ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) + + + INPUT_CHECK ( + ch_input + ) + .map { + meta, fastq -> + meta.id = meta.id.split('_')[0..-2].join('_') + [ meta, fastq ] } + .groupTuple(by: [0]) + .branch { + meta, fastq -> + single : fastq.size() == 1 + return [ meta, fastq.flatten() ] + multiple: fastq.size() > 1 + return [ meta, fastq.flatten() ] + } + .set { ch_fastq } + + // MODULE: Concatenate FastQ files from same sample if required + // + CAT_FASTQ ( + ch_fastq.multiple + ) + .mix(ch_fastq.single) + .set { ch_cat_fastq } + + // + // SUBWORKFLOW: Alignment with STAR and gene/transcript quantification with Salmon + // + ch_genome_bam = Channel.empty() + ch_genome_bam_index = Channel.empty() + ch_samtools_stats = Channel.empty() + ch_samtools_flagstat = Channel.empty() + ch_samtools_idxstats = Channel.empty() + ch_star_multiqc = Channel.empty() + ch_aligner_pca_multiqc = Channel.empty() + ch_aligner_clustering_multiqc = Channel.empty() + if (!params.skip_alignment && params.aligner == 'star_salmon') { + ALIGN_STAR ( + ch_cat_fastq, + PREPARE_GENOME.out.star_index, + PREPARE_GENOME.out.gtf + ) + ch_genome_bam = ALIGN_STAR.out.bam + ch_genome_bam_index = ALIGN_STAR.out.bai + ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript + ch_samtools_stats = ALIGN_STAR.out.stats + ch_samtools_flagstat = ALIGN_STAR.out.flagstat + ch_samtools_idxstats = ALIGN_STAR.out.idxstats + ch_star_multiqc = ALIGN_STAR.out.log_final + if (params.bam_csi_index) { + ch_genome_bam_index = ALIGN_STAR.out.csi + } + ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) + ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) + + } + +} \ No newline at end of file From a935a287f35f79bad4f5bd25a4af07863ba21885 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 4 Jun 2021 16:58:09 +0200 Subject: [PATCH 003/235] sample workflow add with indexing and alignment with star --- lib/WorkflowRnaseq.groovy | 12 ------------ workflows/rnaseq.nf | 4 +--- 2 files changed, 1 insertion(+), 15 deletions(-) diff --git a/lib/WorkflowRnaseq.groovy b/lib/WorkflowRnaseq.groovy index 5cdc71ac..af4b665b 100755 --- a/lib/WorkflowRnaseq.groovy +++ b/lib/WorkflowRnaseq.groovy @@ -45,18 +45,6 @@ class WorkflowRnaseq { skipAlignmentWarn(log) } - if (params.pseudo_aligner) { - if (!valid_params['pseudoaligners'].contains(params.pseudo_aligner)) { - log.error "Invalid option: '${params.pseudo_aligner}'. Valid options for '--pseudo_aligner': ${valid_params['pseudoaligners'].join(', ')}." - System.exit(1) - } else { - if (!(params.salmon_index || params.transcript_fasta || (params.fasta && (params.gtf || params.gff)))) { - log.error "To use `--pseudo_aligner 'salmon'`, you must provide either --salmon_index or --transcript_fasta or both --fasta and --gtf / --gff." - System.exit(1) - } - } - } - // Checks when running --aligner star_rsem if (!params.skip_alignment && params.aligner == 'star_rsem') { if (params.with_umi) { diff --git a/workflows/rnaseq.nf b/workflows/rnaseq.nf index c4f2e7c5..26f0d075 100644 --- a/workflows/rnaseq.nf +++ b/workflows/rnaseq.nf @@ -8,9 +8,7 @@ def valid_params = [ - aligners : ['star_salmon', 'star_rsem', 'hisat2'], - pseudoaligners : ['salmon'], - rseqc_modules : ['bam_stat', 'inner_distance', 'infer_experiment', 'junction_annotation', 'junction_saturation', 'read_distribution', 'read_duplication'] + aligners : ['star_salmon'] ] def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) From 40f0abf8049dd1c471db353471548aaef88a39d9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 4 Jun 2021 17:04:33 +0200 Subject: [PATCH 004/235] sample workflow add with indexing and alignment with star --- lib/WorkflowRnaseq.groovy | 6 ------ workflows/rnaseq.nf | 2 +- 2 files changed, 1 insertion(+), 7 deletions(-) diff --git a/lib/WorkflowRnaseq.groovy b/lib/WorkflowRnaseq.groovy index af4b665b..4b345c56 100755 --- a/lib/WorkflowRnaseq.groovy +++ b/lib/WorkflowRnaseq.groovy @@ -55,12 +55,6 @@ class WorkflowRnaseq { } } - // Check which RSeQC modules we are running - def rseqc_modules = params.rseqc_modules ? params.rseqc_modules.split(',').collect{ it.trim().toLowerCase() } : [] - if ((valid_params['rseqc_modules'] + rseqc_modules).unique().size() != valid_params['rseqc_modules'].size()) { - log.error "Invalid option: ${params.rseqc_modules}. Valid options for '--rseqc_modules': ${valid_params['rseqc_modules'].join(', ')}" - System.exit(1) - } } // diff --git a/workflows/rnaseq.nf b/workflows/rnaseq.nf index 26f0d075..1021ece7 100644 --- a/workflows/rnaseq.nf +++ b/workflows/rnaseq.nf @@ -8,7 +8,7 @@ def valid_params = [ - aligners : ['star_salmon'] + aligners : ['star_salmon', 'star_rsem', 'hisat2'], ] def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) From 9671d7ea9d5e0e1d604b6c6f1a7a39273d9729ad Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 8 Jun 2021 09:55:30 +0200 Subject: [PATCH 005/235] Updated lib/WorkflowRnaseq.groovy Co-authored-by: Maxime U. Garcia --- lib/WorkflowRnaseq.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/WorkflowRnaseq.groovy b/lib/WorkflowRnaseq.groovy index 4b345c56..6d460568 100755 --- a/lib/WorkflowRnaseq.groovy +++ b/lib/WorkflowRnaseq.groovy @@ -2,7 +2,7 @@ // This file holds several functions specific to the workflow/rnaseq.nf in the nf-core/rnaseq pipeline // -class WorkflowRnaseq { +class WorkflowRnavar { // // Check and validate parameters From 59c7d607504b09b6af1975559a381a7dd976d689 Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 8 Jun 2021 12:02:12 +0200 Subject: [PATCH 006/235] Updated assets/samplesheet.valid.csv Co-authored-by: Maxime U. Garcia --- assets/samplesheet.valid.csv | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/samplesheet.valid.csv b/assets/samplesheet.valid.csv index dd979a5d..8c5e16bb 100644 --- a/assets/samplesheet.valid.csv +++ b/assets/samplesheet.valid.csv @@ -1,2 +1,2 @@ -sample,single_end,fastq_1,fastq_2,strandedness +sample,fastq_1,fastq_2,strandedness WT_REP1_T1,0,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_2.fastq.gz,reverse From d5f005e086b7cf3154a38723a223e7e4b8f2c712 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 8 Jun 2021 12:09:15 +0200 Subject: [PATCH 007/235] changes based on PR1 review --- assets/samplesheet_valid.csv | 8 + conf/base.config | 2 +- conf/modules.config | 213 ------------------ lib/WorkflowMain.groovy | 6 +- ...lowRnaseq.groovy => WorkflowRnavar.groovy} | 2 +- main.nf | 4 +- assets/samplesheet.valid.csv => test.csv | 0 workflows/{rnaseq.nf => rnavar.nf} | 4 +- 8 files changed, 18 insertions(+), 221 deletions(-) create mode 100644 assets/samplesheet_valid.csv rename lib/{WorkflowRnaseq.groovy => WorkflowRnavar.groovy} (99%) rename assets/samplesheet.valid.csv => test.csv (100%) rename workflows/{rnaseq.nf => rnavar.nf} (99%) diff --git a/assets/samplesheet_valid.csv b/assets/samplesheet_valid.csv new file mode 100644 index 00000000..ec9e7dba --- /dev/null +++ b/assets/samplesheet_valid.csv @@ -0,0 +1,8 @@ +sample,fastq_1,fastq_2,strandedness +WT_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_2.fastq.gz,reverse +WT_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357071_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357071_2.fastq.gz,reverse +WT_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357072_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357072_2.fastq.gz,reverse +RAP1_UNINDUCED_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357073_1.fastq.gz,,reverse +RAP1_UNINDUCED_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357074_1.fastq.gz,,reverse +RAP1_UNINDUCED_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357075_1.fastq.gz,,reverse +RAP1_IAA_30M_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357076_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357076_2.fastq.gz,reverse diff --git a/conf/base.config b/conf/base.config index 4945e74c..e3d4076a 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ======================================================================================== - nf-core/rnaseq Nextflow base config file + nf-core/rnavar Nextflow base config file ======================================================================================== A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on diff --git a/conf/modules.config b/conf/modules.config index 756e337e..e2fa82bf 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,48 +22,9 @@ params { modules { - 'sra_ids_to_runinfo' { - publish_dir = 'public_data' - publish_files = ['tsv':'runinfo'] - } - 'sra_runinfo_to_ftp' { - publish_dir = 'public_data' - publish_files = ['tsv':'runinfo'] - } - 'sra_fastq_ftp' { - publish_dir = 'public_data' - publish_files = ['fastq.gz':'', 'md5':'md5'] - args = '-C - --max-time 1200' - } - 'sra_to_samplesheet' { - publish_dir = 'public_data' - publish_files = false - } - 'sra_merge_samplesheet' { - publish_dir = 'public_data' - } - 'gffread' { - args = '--keep-exon-attrs -F -T' - publish_dir = 'genome' - } 'cat_fastq' { publish_dir = 'fastq' } - 'fastqc' { - args = '--quiet' - } - 'trimgalore' { - args = '--fastqc' - publish_files = ['txt':'', 'html':'fastqc', 'zip':'fastqc'] - } - 'umitools_extract' { - args = '' - publish_files = ['log':''] - } - 'sortmerna' { - args = '--num_alignments 1 --fastx -v' - publish_files = ['log':''] - } 'star_genomegenerate' { publish_dir = 'genome/index' } @@ -72,179 +33,5 @@ params { publish_dir = "${params.aligner}" publish_files = ['out':'log', 'tab':'log'] } - 'star_salmon_quant' { - publish_dir = "${params.aligner}" - } - 'star_salmon_tximport' { - publish_dir = "${params.aligner}" - publish_by_meta = true - } - 'star_salmon_merge_counts' { - publish_dir = "${params.aligner}" - } - 'hisat2_build' { - publish_dir = 'genome/index' - } - 'hisat2_align' { - args = '--met-stderr --new-summary --dta' - publish_files = ['log':'log'] - } - 'rsem_preparereference' { - args = '--star' - publish_dir = 'genome/index' - } - 'rsem_calculateexpression' { - args = '--star --star-output-genome-bam --star-gzipped-read-file --estimate-rspd --seed 1' - publish_files = ['log':'log', 'stat':'', 'results':''] - publish_dir = "${params.aligner}" - } - 'rsem_merge_counts' { - publish_dir = "${params.aligner}" - } - 'samtools_sort_genome' { - suffix = '.sorted' - publish_files = [:] - publish_dir = "${params.aligner}" - } - 'samtools_index_genome' { - args = '' - suffix = '.sorted' - publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "${params.aligner}" - } - 'samtools_sort_transcriptome' { - suffix = '.transcriptome.sorted' - publish_files = false - } - 'samtools_index_transcriptome' { - suffix = '.transcriptome.sorted' - publish_files = false - } - 'umitools_dedup_genome' { - publish_files = ['tsv':'umitools'] - suffix = '.umi_dedup.sorted' - publish_dir = "${params.aligner}" - } - 'umitools_dedup_genome_samtools' { - args = '' - suffix = '.umi_dedup.sorted' - publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "${params.aligner}" - } - 'umitools_dedup_transcriptome' { - publish_files = ['tsv':'umitools'] - suffix = '.umi_dedup.transcriptome.sorted' - publish_dir = "${params.aligner}" - } - 'umitools_dedup_transcriptome_sort' { - args = '-n' - suffix = '.umi_dedup.transcriptome' - publish_files = false - } - 'salmon_index' { - args = '' - publish_dir = 'genome/index' - } - 'salmon_quant' { - args = '' - } - 'salmon_tximport' { - publish_by_meta = true - } - 'salmon_merge_counts' { - args = '' - } - 'preseq_lcextrap' { - args = '-verbose -bam -seed 1 -seg_len 100000000' - publish_files = ['txt':'', 'log':'log'] - publish_dir = "${params.aligner}/preseq" - } - 'picard_markduplicates' { - args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' - suffix = '.markdup.sorted' - publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] - publish_dir = "${params.aligner}" - } - 'picard_markduplicates_samtools' { - args = '' - suffix = '.markdup.sorted' - publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "${params.aligner}" - } - 'stringtie' { - args = '-v' - publish_dir = "${params.aligner}/stringtie" - } - 'subread_featurecounts' { - args = '-B -C' - publish_dir = "${params.aligner}/featurecounts" - } - 'multiqc_custom_biotype' { - publish_dir = "${params.aligner}/featurecounts" - } - 'bedtools_genomecov' { - args = '-split -du' - publish_files = false - } - 'ucsc_bedclip_sense' { - suffix = '.clip.sense' - publish_files = false - } - 'ucsc_bedclip_antisense' { - suffix = '.clip.antisense' - publish_files = false - } - 'ucsc_bedgraphtobigwig_sense' { - suffix = '.sense' - publish_dir = "${params.aligner}/bigwig" - } - 'ucsc_bedgraphtobigwig_antisense' { - suffix = '.antisense' - publish_dir = "${params.aligner}/bigwig" - } - 'deseq2_qc' { - args = "--id_col 1 --sample_suffix '' --outprefix deseq2 --count_col 3" - publish_files = ['RData':'', 'pca.vals.txt':'', 'plots.pdf':'', 'sample.dists.txt':'', 'size_factors':'', 'log':''] - publish_dir = "${params.aligner}/deseq2_qc" - } - 'qualimap_rnaseq' { - publish_dir = "${params.aligner}/qualimap" - } - 'dupradar' { - publish_files = [ - 'Dens.pdf':'scatter_plot', 'Boxplot.pdf':'box_plot', 'Hist.pdf':'histogram', - 'Matrix.txt':'gene_data', 'slope.txt':'intercepts_slope' - ] - publish_dir = "${params.aligner}/dupradar" - } - 'rseqc_bamstat' { - publish_dir = "${params.aligner}/rseqc/bam_stat" - } - 'rseqc_innerdistance' { - publish_files = ['txt':'txt', 'pdf':'pdf', '.r':'rscript'] - publish_dir = "${params.aligner}/rseqc/inner_distance" - } - 'rseqc_inferexperiment' { - publish_dir = "${params.aligner}/rseqc/infer_experiment" - } - 'rseqc_junctionannotation' { - publish_files = ['pdf':'pdf', '.r':'rscript', 'bed':'bed', 'xls':'xls', 'log':'log'] - publish_dir = "${params.aligner}/rseqc/junction_annotation" - } - 'rseqc_junctionsaturation' { - publish_files = ['pdf':'pdf', '.r':'rscript'] - publish_dir = "${params.aligner}/rseqc/junction_saturation" - } - 'rseqc_readdistribution' { - publish_dir = "${params.aligner}/rseqc/read_distribution" - } - 'rseqc_readduplication' { - publish_files = ['pdf':'pdf', '.r':'rscript', 'xls':'xls'] - publish_dir = "${params.aligner}/rseqc/read_duplication" - } - 'multiqc' { - args = '' - publish_dir = "multiqc/${params.aligner}" - } } } diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 085dbd3d..741156c4 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -8,13 +8,15 @@ class WorkflowMain { // Citation string for pipeline // public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + return "Unpublished" + /* + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + " https://doi.org/10.5281/zenodo.1400710\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" */ } // diff --git a/lib/WorkflowRnaseq.groovy b/lib/WorkflowRnavar.groovy similarity index 99% rename from lib/WorkflowRnaseq.groovy rename to lib/WorkflowRnavar.groovy index 4b345c56..6d460568 100755 --- a/lib/WorkflowRnaseq.groovy +++ b/lib/WorkflowRnavar.groovy @@ -2,7 +2,7 @@ // This file holds several functions specific to the workflow/rnaseq.nf in the nf-core/rnaseq pipeline // -class WorkflowRnaseq { +class WorkflowRnavar { // // Check and validate parameters diff --git a/main.nf b/main.nf index c6a2f477..be192e6c 100644 --- a/main.nf +++ b/main.nf @@ -39,11 +39,11 @@ RUN THE WORKFLOW */ workflow { - include { RNASEQ_VAR } from './workflows/rnaseq' + include { RNASEQ_VAR } from './workflows/rnavar' RNASEQ_VAR () } /* END OF THE FILE -*/ \ No newline at end of file +*/ diff --git a/assets/samplesheet.valid.csv b/test.csv similarity index 100% rename from assets/samplesheet.valid.csv rename to test.csv diff --git a/workflows/rnaseq.nf b/workflows/rnavar.nf similarity index 99% rename from workflows/rnaseq.nf rename to workflows/rnavar.nf index 1021ece7..48b90dd8 100644 --- a/workflows/rnaseq.nf +++ b/workflows/rnavar.nf @@ -14,7 +14,7 @@ def valid_params = [ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters -WorkflowRnaseq.initialise(params, log, valid_params) +WorkflowRnavar.initialise(params, log, valid_params) // Check input path parameters to see if they exist checkPathParamList = [ @@ -140,4 +140,4 @@ workflow RNASEQ_VAR { } -} \ No newline at end of file +} From e48d76517dd91aaff75668ce88c6e6c7cf4ab977 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 8 Jun 2021 13:12:23 +0200 Subject: [PATCH 008/235] changes based on PR1 review --- test.csv | 2 -- 1 file changed, 2 deletions(-) delete mode 100644 test.csv diff --git a/test.csv b/test.csv deleted file mode 100644 index 8c5e16bb..00000000 --- a/test.csv +++ /dev/null @@ -1,2 +0,0 @@ -sample,fastq_1,fastq_2,strandedness -WT_REP1_T1,0,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_2.fastq.gz,reverse From 4c66a9aa9003a5d2df12d02ebf5c4e869ffb2277 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 8 Jun 2021 13:24:02 +0200 Subject: [PATCH 009/235] changes based on PR1 review --- conf/modules.config | 11 +++++++++++ 1 file changed, 11 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index e2fa82bf..ad0a41dc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -33,5 +33,16 @@ params { publish_dir = "${params.aligner}" publish_files = ['out':'log', 'tab':'log'] } + 'samtools_sort_genome' { + suffix = '.sorted' + publish_files = [:] + publish_dir = "${params.aligner}" + } + 'samtools_index_genome' { + args = '' + suffix = '.sorted' + publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "${params.aligner}" + } } } From cb942643797d6ce97abdecc50173c4f5d4cd84f0 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 15 Jun 2021 12:48:44 +0200 Subject: [PATCH 010/235] Added steps upto SplitNCigar Reads --- conf/modules.config | 26 ++++++- .../gatk4/baserecalibrator/functions.nf | 68 ++++++++++++++++ .../software/gatk4/baserecalibrator/main.nf | 50 ++++++++++++ .../software/gatk4/baserecalibrator/meta.yml | 58 ++++++++++++++ .../software/gatk4/bedtointervallist/main.nf | 14 ++-- .../createsequencedictionary/functions.nf | 68 ++++++++++++++++ .../gatk4/createsequencedictionary/main.nf | 45 +++++++++++ .../gatk4/createsequencedictionary/meta.yml | 30 +++++++ .../gatk4/splitncigarreads/functions.nf | 68 ++++++++++++++++ .../software/gatk4/splitncigarreads/main.nf | 45 +++++++++++ .../software/gatk4/splitncigarreads/meta.yml | 40 ++++++++++ .../picard/markduplicates/functions.nf | 68 ++++++++++++++++ .../software/picard/markduplicates/main.nf | 49 ++++++++++++ .../software/picard/markduplicates/meta.yml | 46 +++++++++++ .../software/samtools/faidx/functions.nf | 68 ++++++++++++++++ .../nf-core/software/samtools/faidx/main.nf | 34 ++++++++ .../nf-core/software/samtools/faidx/meta.yml | 32 ++++++++ nextflow.config | 2 + subworkflows/local/prepare_genome.nf | 11 +++ .../nf-core/mark_duplicates_picard.nf | 55 +++++++++++++ subworkflows/nf-core/splitn_cigar_reads.nf | 55 +++++++++++++ workflows/rnavar.nf | 78 +++++++++++++++++-- 22 files changed, 996 insertions(+), 14 deletions(-) create mode 100644 modules/nf-core/software/gatk4/baserecalibrator/functions.nf create mode 100644 modules/nf-core/software/gatk4/baserecalibrator/main.nf create mode 100644 modules/nf-core/software/gatk4/baserecalibrator/meta.yml create mode 100644 modules/nf-core/software/gatk4/createsequencedictionary/functions.nf create mode 100644 modules/nf-core/software/gatk4/createsequencedictionary/main.nf create mode 100644 modules/nf-core/software/gatk4/createsequencedictionary/meta.yml create mode 100644 modules/nf-core/software/gatk4/splitncigarreads/functions.nf create mode 100644 modules/nf-core/software/gatk4/splitncigarreads/main.nf create mode 100644 modules/nf-core/software/gatk4/splitncigarreads/meta.yml create mode 100644 modules/nf-core/software/picard/markduplicates/functions.nf create mode 100644 modules/nf-core/software/picard/markduplicates/main.nf create mode 100644 modules/nf-core/software/picard/markduplicates/meta.yml create mode 100644 modules/nf-core/software/samtools/faidx/functions.nf create mode 100644 modules/nf-core/software/samtools/faidx/main.nf create mode 100644 modules/nf-core/software/samtools/faidx/meta.yml create mode 100644 subworkflows/nf-core/mark_duplicates_picard.nf create mode 100644 subworkflows/nf-core/splitn_cigar_reads.nf diff --git a/conf/modules.config b/conf/modules.config index ad0a41dc..29933352 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,7 +29,7 @@ params { publish_dir = 'genome/index' } 'star_align' { - args = '--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend' + args = '--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang 100' publish_dir = "${params.aligner}" publish_files = ['out':'log', 'tab':'log'] } @@ -43,6 +43,30 @@ params { suffix = '.sorted' publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] publish_dir = "${params.aligner}" + } + 'picard_markduplicates' { + args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' + suffix = '.markdup.sorted' + publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] + publish_dir = "${params.aligner}" } + 'picard_markduplicates_samtools' { + args = '' + suffix = '.markdup.sorted' + publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "${params.aligner}" + } + 'gatk_splitncigar_options' { + args = '' + suffix = '.split_cigar.bam' + publish_files = ['bam': '', 'bai': ''] + publish_dir = "${params.aligner}" + } + 'gatk_baserecalibrator' { + args = '' + suffix = '' + publish_files = [:] + publish_dir = "${params.aligner}/recalibrated" + } } } diff --git a/modules/nf-core/software/gatk4/baserecalibrator/functions.nf b/modules/nf-core/software/gatk4/baserecalibrator/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/baserecalibrator/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/baserecalibrator/main.nf b/modules/nf-core/software/gatk4/baserecalibrator/main.nf new file mode 100644 index 00000000..31ffbd20 --- /dev/null +++ b/modules/nf-core/software/gatk4/baserecalibrator/main.nf @@ -0,0 +1,50 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_BASERECALIBRATOR { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam), path(bai) + path fasta + path fastaidx + path dict + path intervalsBed + path knownSites + path knownSites_tbi + + output: + tuple val(meta), path("*.table"), emit: table + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') + """ + gatk BaseRecalibrator \ + -R $fasta \ + -I $bam \ + $sitesCommand \ + $intervalsCommand \ + $options.args \ + -O ${prefix}.table + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/baserecalibrator/meta.yml b/modules/nf-core/software/gatk4/baserecalibrator/meta.yml new file mode 100644 index 00000000..0996dcbe --- /dev/null +++ b/modules/nf-core/software/gatk4/baserecalibrator/meta.yml @@ -0,0 +1,58 @@ +name: gatk4_baserecalibrator +description: Generate recalibration table for Base Quality Score Recalibration (BQSR) +keywords: + - sort +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file from alignment + pattern: "*.{bam}" + - fasta: + type: file + description: The reference fasta file + - fastaidx: + type: file + description: Index of reference fasta file + - dict: + type: file + description: GATK sequence dictionary + - intervalsBed: + type: file + description: Bed file with the genomic regions included in the library (optional) + - knownSites: + type: file + description: Bed file with the genomic regions included in the library (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - table: + type: file + description: Recalibration table from BaseRecalibrator + pattern: "*.{table}" + +authors: + - "@yocra3" diff --git a/modules/nf-core/software/gatk4/bedtointervallist/main.nf b/modules/nf-core/software/gatk4/bedtointervallist/main.nf index 284f2dfc..0f8021e1 100644 --- a/modules/nf-core/software/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/software/gatk4/bedtointervallist/main.nf @@ -1,3 +1,5 @@ +nextflow.enable.dsl=2 + // Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' @@ -5,11 +7,11 @@ params.options = [:] options = initOptions(params.options) process GATK4_BEDTOINTERVALLIST { - tag "$meta.id" + tag "$bed" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -19,16 +21,16 @@ process GATK4_BEDTOINTERVALLIST { } input: - tuple val(meta), path(bed) + path bed path sequence_dict output: - tuple val(meta), path('*.interval_list'), emit: interval_list - path '*.version.txt' , emit: version + path '*.interval_list' , emit: interval_list + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${bed}.${options.suffix}" : "${bed}" """ gatk BedToIntervalList \\ -I $bed \\ diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/functions.nf b/modules/nf-core/software/gatk4/createsequencedictionary/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/createsequencedictionary/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/main.nf b/modules/nf-core/software/gatk4/createsequencedictionary/main.nf new file mode 100644 index 00000000..6997fb5f --- /dev/null +++ b/modules/nf-core/software/gatk4/createsequencedictionary/main.nf @@ -0,0 +1,45 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_CREATESEQUENCEDICTIONARY { + tag "$fasta" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::gatk4=4.1.9.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0" + } else { + container "quay.io/biocontainers/gatk4:4.1.9.0--py39_0" + } + + input: + path fasta + + output: + path "*.dict" , emit: dict + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def avail_mem = 6 + if (!task.memory) { + log.info '[GATK] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk --java-options "-Xmx${avail_mem}g" \\ + CreateSequenceDictionary \\ + --REFERENCE $fasta \\ + --URI $fasta \\ + $options.args + + echo \$(gatk CreateSequenceDictionary --version 2>&1) | sed 's/^.*(GATK) v//; s/ HTSJDK.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/software/gatk4/createsequencedictionary/meta.yml new file mode 100644 index 00000000..d0cc5980 --- /dev/null +++ b/modules/nf-core/software/gatk4/createsequencedictionary/meta.yml @@ -0,0 +1,30 @@ +name: gatk4_createsequencedictionary +description: Creates a sequence dictionary for a reference sequence +keywords: + - dictionary + - fasta +tools: + - gatk: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - fasta: + type: file + description: Input fasta file + pattern: "*.{fasta,fa}" +output: + - dict: + type: file + description: gatk dictionary file + pattern: "*.{dict}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@maxulysse" diff --git a/modules/nf-core/software/gatk4/splitncigarreads/functions.nf b/modules/nf-core/software/gatk4/splitncigarreads/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/splitncigarreads/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/splitncigarreads/main.nf b/modules/nf-core/software/gatk4/splitncigarreads/main.nf new file mode 100644 index 00000000..8be9efde --- /dev/null +++ b/modules/nf-core/software/gatk4/splitncigarreads/main.nf @@ -0,0 +1,45 @@ +nextflow.enable.dsl=2 + +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_SPLITNCIGARREADS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + } else { + container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + } + + input: + tuple val(meta), path(bam) + path fasta + path fai + path dict + + output: + tuple val(meta), path('*.split_cigar.bam'), emit: bam + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + """ + gatk SplitNCigarReads \\ + -R $fasta \\ + -I $bam \\ + -O ${prefix}.split_cigar.bam \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/splitncigarreads/meta.yml b/modules/nf-core/software/gatk4/splitncigarreads/meta.yml new file mode 100644 index 00000000..e433cbf6 --- /dev/null +++ b/modules/nf-core/software/gatk4/splitncigarreads/meta.yml @@ -0,0 +1,40 @@ +name: gatk4_splitncigarreads +description: Splits reads that contain Ns in their cigar string +keywords: + - vcf + - merge +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - bam: + type: list + description: BAM/SAM/CRAM file containing reads + pattern: "*.{bam,sam,cram}" + - fasta: + type: tuple of files + description: | + Tuple of fasta file (first), sequence dict (second) and fasta index (third) + pattern: ["*.fasta", "*.dict", "*.fai"] +output: + - bam: + type: file + description: Output file with split reads (BAM/SAM/CRAM) + pattern: "*.{bam,sam,cram}" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/modules/nf-core/software/picard/markduplicates/functions.nf b/modules/nf-core/software/picard/markduplicates/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/picard/markduplicates/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/picard/markduplicates/main.nf b/modules/nf-core/software/picard/markduplicates/main.nf new file mode 100644 index 00000000..d7647414 --- /dev/null +++ b/modules/nf-core/software/picard/markduplicates/main.nf @@ -0,0 +1,49 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process PICARD_MARKDUPLICATES { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::picard=2.23.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/picard:2.23.9--0" + } else { + container "quay.io/biocontainers/picard:2.23.9--0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam") , emit: bam + tuple val(meta), path("*.metrics.txt"), emit: metrics + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + picard \\ + -Xmx${avail_mem}g \\ + MarkDuplicates \\ + $options.args \\ + INPUT=$bam \\ + OUTPUT=${prefix}.bam \\ + METRICS_FILE=${prefix}.MarkDuplicates.metrics.txt + + echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d: > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/picard/markduplicates/meta.yml b/modules/nf-core/software/picard/markduplicates/meta.yml new file mode 100644 index 00000000..6420ce9a --- /dev/null +++ b/modules/nf-core/software/picard/markduplicates/meta.yml @@ -0,0 +1,46 @@ +name: picard_markduplicates +description: Locate and tag duplicate reads in a BAM file +keywords: + - markduplicates + - pcr + - duplicates + - bam + - sam + - cram +tools: + - picard: + description: | + A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) + data and formats such as SAM/BAM/CRAM and VCF. + homepage: https://broadinstitute.github.io/picard/ + documentation: https://broadinstitute.github.io/picard/ +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.{bam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file with duplicate reads marked/removed + pattern: "*.{bam}" + - metrics: + type: file + description: Duplicate metrics file generated by picard + pattern: "*.{metrics.txt}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" diff --git a/modules/nf-core/software/samtools/faidx/functions.nf b/modules/nf-core/software/samtools/faidx/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/faidx/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/faidx/main.nf b/modules/nf-core/software/samtools/faidx/main.nf new file mode 100644 index 00000000..6c023f1c --- /dev/null +++ b/modules/nf-core/software/samtools/faidx/main.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_FAIDX { + tag "$fasta" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::samtools=1.12" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.12--hd5e65b6_0" + } else { + container "quay.io/biocontainers/samtools:1.12--hd5e65b6_0" + } + + input: + path fasta + + output: + path "*.fai" , emit: fai + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + """ + samtools faidx $fasta + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/faidx/meta.yml b/modules/nf-core/software/samtools/faidx/meta.yml new file mode 100644 index 00000000..f92234d0 --- /dev/null +++ b/modules/nf-core/software/samtools/faidx/meta.yml @@ -0,0 +1,32 @@ +name: samtools_faidx +description: Index FASTA file +keywords: + - index + - fasta +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - fasta: + type: file + description: FASTA file + pattern: "*.{fa,fasta}" +output: + - fai: + type: file + description: FASTA index file + pattern: "*.{fai}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@ewels" + - "@phue" diff --git a/nextflow.config b/nextflow.config index 59e794ae..ff98f8e7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -60,6 +60,8 @@ params { save_align_intermeds = false skip_markduplicates = false skip_alignment = false + skip_splitncigar = false + skip_basecalibrator = false // QC skip_qc = false diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 60064264..2c6a792b 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -33,6 +33,8 @@ include { GTF2BED } from '../../modules/local/gtf2bed' include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams( options: params.genome_options ) include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams( options: params.genome_options ) include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams( options: params.genome_options ) +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/software/samtools/faidx/main' addParams( options: params.genome_options ) +include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/software/gatk4/createsequencedictionary/main' addParams( options: params.genome_options ) workflow PREPARE_GENOME { take: @@ -113,6 +115,13 @@ workflow PREPARE_GENOME { ch_rsem_version = RSEM_PREPAREREFERENCE_TRANSCRIPTS.out.version } + // Index the genome fasta + ch_fasta_fai = Channel.empty() + ch_fasta_fai = SAMTOOLS_FAIDX ( ch_fasta ).fai + + // Create dictionary file for the genome fasta + ch_fasta_dict = Channel.empty() + ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY ( ch_fasta ).dict // // Create chromosome sizes file // @@ -198,6 +207,8 @@ workflow PREPARE_GENOME { emit: fasta = ch_fasta // path: genome.fasta + fai = ch_fasta_fai // path: genome.fasta.fai + dict = ch_fasta_dict // path: genome.fasta.dict gtf = ch_gtf // path: genome.gtf gene_bed = ch_gene_bed // path: gene.bed transcript_fasta = ch_transcript_fasta // path: transcript.fasta diff --git a/subworkflows/nf-core/mark_duplicates_picard.nf b/subworkflows/nf-core/mark_duplicates_picard.nf new file mode 100644 index 00000000..7e59ac68 --- /dev/null +++ b/subworkflows/nf-core/mark_duplicates_picard.nf @@ -0,0 +1,55 @@ +// +// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// + +params.markduplicates_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/software/picard/markduplicates/main' addParams( options: params.markduplicates_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.samtools_index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) + +workflow MARK_DUPLICATES_PICARD { + take: + bam // channel: [ val(meta), [ bam ] ] + + main: + + // + // Picard MarkDuplicates + // + PICARD_MARKDUPLICATES ( bam ) + + // + // Index BAM file and run samtools stats, flagstat and idxstats + // + SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + + PICARD_MARKDUPLICATES.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai ) + + emit: + bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] + picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt + + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf new file mode 100644 index 00000000..7e59ac68 --- /dev/null +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -0,0 +1,55 @@ +// +// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// + +params.markduplicates_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/software/picard/markduplicates/main' addParams( options: params.markduplicates_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.samtools_index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) + +workflow MARK_DUPLICATES_PICARD { + take: + bam // channel: [ val(meta), [ bam ] ] + + main: + + // + // Picard MarkDuplicates + // + PICARD_MARKDUPLICATES ( bam ) + + // + // Index BAM file and run samtools stats, flagstat and idxstats + // + SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + + PICARD_MARKDUPLICATES.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai ) + + emit: + bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] + picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt + + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt +} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 48b90dd8..20972ab0 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -8,7 +8,7 @@ def valid_params = [ - aligners : ['star_salmon', 'star_rsem', 'hisat2'], + aligners : ['star_salmon'], ] def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) @@ -60,18 +60,26 @@ if (['star_salmon','hisat2'].contains(params.aligner)) { def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type +def cat_fastq_options = modules['cat_fastq'] +if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } + def star_align_options = modules['star_align'] star_align_options.args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' if (params.save_align_intermeds) { star_align_options.publish_files.put('bam','') } if (params.save_unaligned) { star_align_options.publish_files.put('fastq.gz','unmapped') } -def cat_fastq_options = modules['cat_fastq'] -if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } +def picard_markduplicates_samtools = modules['picard_markduplicates_samtools'] +picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/software/gatk4/bedtointervallist/main' addParams( options: [:]) +include { SAMTOOLS_INDEX } from '../modules/nf-core/software/samtools/index/main' addParams( options: samtools_index_genome_options ) include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) +include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) +include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) workflow RNASEQ_VAR { @@ -79,6 +87,8 @@ workflow RNASEQ_VAR { prepareToolIndices, biotype ) + ch_genome_gtf = Channel.empty() + ch_genome_gtf = PREPARE_GENOME.out.gene_bed ch_software_versions = Channel.empty() ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) @@ -108,8 +118,16 @@ workflow RNASEQ_VAR { .mix(ch_fastq.single) .set { ch_cat_fastq } + // PREPARE THE INTERVAL LIST + ch_interval_list = Channel.empty() + GATK4_BEDTOINTERVALLIST( + ch_genome_gtf, + PREPARE_GENOME.out.dict + ) + ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list + ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) // - // SUBWORKFLOW: Alignment with STAR and gene/transcript quantification with Salmon + // SUBWORKFLOW: Alignment with STAR // ch_genome_bam = Channel.empty() ch_genome_bam_index = Channel.empty() @@ -119,6 +137,7 @@ workflow RNASEQ_VAR { ch_star_multiqc = Channel.empty() ch_aligner_pca_multiqc = Channel.empty() ch_aligner_clustering_multiqc = Channel.empty() + if (!params.skip_alignment && params.aligner == 'star_salmon') { ALIGN_STAR ( ch_cat_fastq, @@ -138,6 +157,51 @@ workflow RNASEQ_VAR { ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) - } + // SUBWORKFLOW: Mark duplicates with Picard + ch_markduplicates_multiqc = Channel.empty() + if (!params.skip_alignment && !params.skip_markduplicates) { + MARK_DUPLICATES_PICARD ( + ch_genome_bam + ) + ch_genome_bam = MARK_DUPLICATES_PICARD.out.bam + ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.bai + ch_samtools_stats = MARK_DUPLICATES_PICARD.out.stats + ch_samtools_flagstat = MARK_DUPLICATES_PICARD.out.flagstat + ch_samtools_idxstats = MARK_DUPLICATES_PICARD.out.idxstats + ch_markduplicates_multiqc = MARK_DUPLICATES_PICARD.out.metrics + if (params.bam_csi_index) { + ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.csi + } + ch_software_versions = ch_software_versions.mix(MARK_DUPLICATES_PICARD.out.picard_version.first().ifEmpty(null)) + } + // MODULE: SplitNCigarReads from GATK4 + // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). -} + if(!params.skip_splitncigar){ + + GATK4_SPLITNCIGARREADS ( + ch_genome_bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict + ) + + SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) + + ch_genome_bam = GATK4_SPLITNCIGARREADS.out.bam + ch_genome_bam_index = SAMTOOLS_INDEX.out.bai + ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) + } + + /* + if(!params.skip_basecalibrator){ + GATK4_BASERECALIBRATOR( + ch_genome_bam.join(ch_genome_bam_index, by: [0]), + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list, + ) + }*/ + } +} \ No newline at end of file From 24fc548854274c032231f172ab0b775268f8f967 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 15 Jun 2021 12:58:52 +0200 Subject: [PATCH 011/235] Added steps upto SplitNCigar Reads --- lib/WorkflowRnavar.groovy | 2 +- nextflow.config | 2 +- nextflow_schema.json | 6 +++--- subworkflows/local/prepare_genome.nf | 2 +- workflows/rnavar.nf | 6 +++--- 5 files changed, 9 insertions(+), 9 deletions(-) diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 6d460568..06f39141 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -224,7 +224,7 @@ class WorkflowRnavar { " When using '--aligner star_rsem', STAR is run by RSEM itself and so it is\n" + " not possible to remove UMIs before the quantification.\n\n" + " If you would like to remove UMI barcodes using the '--with_umi' option\n" + - " please use either '--aligner star_salmon' or '--aligner hisat2'.\n" + + " please use either '--aligner star' or '--aligner hisat2'.\n" + "=============================================================================" System.exit(1) } diff --git a/nextflow.config b/nextflow.config index ff98f8e7..57534f06 100644 --- a/nextflow.config +++ b/nextflow.config @@ -47,7 +47,7 @@ params { ribo_database_manifest = "${projectDir}/assets/rrna-db-defaults.txt" // Alignment - aligner = 'star_salmon' + aligner = 'star' pseudo_aligner = null seq_center = null bam_csi_index = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 28ed6abb..750f3148 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -331,11 +331,11 @@ "properties": { "aligner": { "type": "string", - "default": "star_salmon", - "description": "Specifies the alignment algorithm to use - available options are 'star_salmon', 'star_rsem' and 'hisat2'.", + "default": "star", + "description": "Specifies the alignment algorithm to use - available options are 'star', 'star_rsem' and 'hisat2'.", "fa_icon": "fas fa-map-signs", "enum": [ - "star_salmon", + "star", "star_rsem", "hisat2" ] diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 2c6a792b..431cf0d4 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -132,7 +132,7 @@ workflow PREPARE_GENOME { // ch_star_index = Channel.empty() ch_star_version = Channel.empty() - if ('star_salmon' in prepare_tool_indices) { + if ('star' in prepare_tool_indices) { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 20972ab0..819e4073 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -8,7 +8,7 @@ def valid_params = [ - aligners : ['star_salmon'], + aligners : ['star'], ] def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) @@ -44,7 +44,7 @@ if (!params.save_reference) { star_genomegenerate_options['publish_files'] = def samtools_sort_genome_options = modules['samtools_sort_genome'] def samtools_index_genome_options = modules['samtools_index_genome'] samtools_index_genome_options.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -if (['star_salmon','hisat2'].contains(params.aligner)) { +if (['star'].contains(params.aligner)) { if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { samtools_sort_genome_options.publish_files.put('bam','') samtools_index_genome_options.publish_files.put('bai','') @@ -138,7 +138,7 @@ workflow RNASEQ_VAR { ch_aligner_pca_multiqc = Channel.empty() ch_aligner_clustering_multiqc = Channel.empty() - if (!params.skip_alignment && params.aligner == 'star_salmon') { + if (!params.skip_alignment && params.aligner == 'star') { ALIGN_STAR ( ch_cat_fastq, PREPARE_GENOME.out.star_index, From d49fad685230b91179e4393f239b76ddbc96cf18 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Jun 2021 10:33:34 +0200 Subject: [PATCH 012/235] Added haplotype caller. Add a new sample sheet in assets to use new rnaseq data from repo --- .github/workflows/ci.yml | 19 +----- .github/workflows/push_dockerhub_dev.yml | 28 -------- .github/workflows/push_dockerhub_release.yml | 29 -------- assets/samplesheet_valid.csv | 8 +-- conf/modules.config | 20 +++++- conf/test.config | 28 +++++--- .../software/gatk4/applybqsr/functions.nf | 68 +++++++++++++++++++ .../nf-core/software/gatk4/applybqsr/main.nf | 49 +++++++++++++ .../nf-core/software/gatk4/applybqsr/meta.yml | 58 ++++++++++++++++ .../software/gatk4/baserecalibrator/main.nf | 10 ++- .../gatk4/haplotypecaller/functions.nf | 68 +++++++++++++++++++ .../software/gatk4/haplotypecaller/main.nf | 54 +++++++++++++++ .../software/gatk4/haplotypecaller/meta.yml | 63 +++++++++++++++++ .../gatk4/intervallisttools/functions.nf | 68 +++++++++++++++++++ .../software/gatk4/intervallisttools/main.nf | 53 +++++++++++++++ .../software/gatk4/intervallisttools/meta.yml | 46 +++++++++++++ modules/nf-core/software/star/align/main.nf | 6 +- nextflow.config | 6 ++ subworkflows/local/prepare_genome.nf | 13 +++- workflows/rnavar.nf | 39 ++++++++++- 20 files changed, 630 insertions(+), 103 deletions(-) delete mode 100644 .github/workflows/push_dockerhub_dev.yml delete mode 100644 .github/workflows/push_dockerhub_release.yml create mode 100644 modules/nf-core/software/gatk4/applybqsr/functions.nf create mode 100644 modules/nf-core/software/gatk4/applybqsr/main.nf create mode 100644 modules/nf-core/software/gatk4/applybqsr/meta.yml create mode 100644 modules/nf-core/software/gatk4/haplotypecaller/functions.nf create mode 100644 modules/nf-core/software/gatk4/haplotypecaller/main.nf create mode 100644 modules/nf-core/software/gatk4/haplotypecaller/meta.yml create mode 100644 modules/nf-core/software/gatk4/intervallisttools/functions.nf create mode 100644 modules/nf-core/software/gatk4/intervallisttools/main.nf create mode 100644 modules/nf-core/software/gatk4/intervallisttools/meta.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9569876a..c14a4d69 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -27,24 +27,7 @@ jobs: steps: - name: Check out pipeline code uses: actions/checkout@v2 - - - name: Check if Dockerfile or Conda environment changed - uses: technote-space/get-diff-action@v4 - with: - FILES: | - Dockerfile - environment.yml - - - name: Build new docker image - if: env.MATCHED_FILES - run: docker build --no-cache . -t nfcore/rnavar:dev - - - name: Pull docker image - if: ${{ !env.MATCHED_FILES }} - run: | - docker pull nfcore/rnavar:dev - docker tag nfcore/rnavar:dev nfcore/rnavar:dev - + - name: Install Nextflow env: CAPSULE_LOG: none diff --git a/.github/workflows/push_dockerhub_dev.yml b/.github/workflows/push_dockerhub_dev.yml deleted file mode 100644 index 385144b0..00000000 --- a/.github/workflows/push_dockerhub_dev.yml +++ /dev/null @@ -1,28 +0,0 @@ -name: nf-core Docker push (dev) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - push: - branches: - - dev - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (dev) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: ${{ github.repository == 'nf-core/rnavar' }} - env: - DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} - DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t nfcore/rnavar:dev - - - name: Push Docker image to DockerHub (dev) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push nfcore/rnavar:dev diff --git a/.github/workflows/push_dockerhub_release.yml b/.github/workflows/push_dockerhub_release.yml deleted file mode 100644 index 1f4f32b9..00000000 --- a/.github/workflows/push_dockerhub_release.yml +++ /dev/null @@ -1,29 +0,0 @@ -name: nf-core Docker push (release) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - release: - types: [published] - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (release) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: ${{ github.repository == 'nf-core/rnavar' }} - env: - DOCKERHUB_USERNAME: ${{ secrets.DOCKERHUB_USERNAME }} - DOCKERHUB_PASS: ${{ secrets.DOCKERHUB_PASS }} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t nfcore/rnavar:latest - - - name: Push Docker image to DockerHub (release) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push nfcore/rnavar:latest - docker tag nfcore/rnavar:latest nfcore/rnavar:${{ github.event.release.tag_name }} - docker push nfcore/rnavar:${{ github.event.release.tag_name }} diff --git a/assets/samplesheet_valid.csv b/assets/samplesheet_valid.csv index ec9e7dba..7131bd5d 100644 --- a/assets/samplesheet_valid.csv +++ b/assets/samplesheet_valid.csv @@ -1,8 +1,2 @@ sample,fastq_1,fastq_2,strandedness -WT_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357070_2.fastq.gz,reverse -WT_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357071_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357071_2.fastq.gz,reverse -WT_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357072_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357072_2.fastq.gz,reverse -RAP1_UNINDUCED_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357073_1.fastq.gz,,reverse -RAP1_UNINDUCED_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357074_1.fastq.gz,,reverse -RAP1_UNINDUCED_REP2,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357075_1.fastq.gz,,reverse -RAP1_IAA_30M_REP1,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357076_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/testdata/GSE110004/SRR6357076_2.fastq.gz,reverse +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/conf/modules.config b/conf/modules.config index 29933352..498b9acc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,7 +29,7 @@ params { publish_dir = 'genome/index' } 'star_align' { - args = '--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang 100' + args = '--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang 149' publish_dir = "${params.aligner}" publish_files = ['out':'log', 'tab':'log'] } @@ -68,5 +68,23 @@ params { publish_files = [:] publish_dir = "${params.aligner}/recalibrated" } + 'gatk_applybqsr' { + args = '' + suffix = '' + publish_files = [:] + publish_dir = "${params.aligner}/recalibrated" + } + 'gatk_haplotypecaller' { + args = '-dont-use-soft-clipped-bases --standard-min-confidence-threshold-for-calling 20' + suffix = '' + publish_files = [:] + publish_dir = "${params.aligner}/variants" + } + 'gatk_intervallisttools' { + args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' + suffix = '' + publish_files = [:] + publish_dir = "${params.aligner}/interval_lists" + } } } diff --git a/conf/test.config b/conf/test.config index e7610dd0..2a53c2da 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,19 +20,25 @@ params { max_time = 6.h // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_test.csv' + input = 'assets/samplesheet_valid.csv' // Genome references - fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genome.fa' - gtf = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gtf.gz' - gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' - transcript_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/transcriptome.fasta' - additional_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/gfp.fa.gz' - - hisat2_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/hisat2.tar.gz' - star_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/star.tar.gz' - salmon_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/salmon.tar.gz' - rsem_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/rsem.tar.gz' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' + fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' + fasta_dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' + gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' + gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' + transcript_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/transcriptome.fasta' + + dbsnp_vcf = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' + dbsnp_vcf_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' + known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' + known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' + + hisat2_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/hisat2.tar.gz' + star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' + salmon_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/salmon.tar.gz' + rsem_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/rsem.tar.gz' // Other parameters pseudo_aligner = 'salmon' diff --git a/modules/nf-core/software/gatk4/applybqsr/functions.nf b/modules/nf-core/software/gatk4/applybqsr/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/applybqsr/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/applybqsr/main.nf b/modules/nf-core/software/gatk4/applybqsr/main.nf new file mode 100644 index 00000000..078c5ad5 --- /dev/null +++ b/modules/nf-core/software/gatk4/applybqsr/main.nf @@ -0,0 +1,49 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_APPLYBQSR { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam), path(bai) + path bqsr_table + path fasta + path fastaidx + path dict + path intervalsBed + + output: + tuple val(meta), path("*.bam"), emit: bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + + """ + gatk ApplyBQSR \\ + -R $fasta \\ + -I $bam \\ + --bqsr-recal-file $bqsr_table \\ + $intervalsCommand \\ + -O ${prefix}.recalibrated.bam \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/applybqsr/meta.yml b/modules/nf-core/software/gatk4/applybqsr/meta.yml new file mode 100644 index 00000000..9bf12f09 --- /dev/null +++ b/modules/nf-core/software/gatk4/applybqsr/meta.yml @@ -0,0 +1,58 @@ +name: gatk4_applybqsr +description: Apply base quality score recalibration (BQSR) to a bam file +keywords: + - bqsr + - bam +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file from alignment + pattern: "*.{bam}" + - bqsr_table: + type: file + description: Recalibration table from gatk4_baserecalibrator + - fasta: + type: file + description: The reference fasta file + - fastaidx: + type: file + description: Index of reference fasta file + - dict: + type: file + description: GATK sequence dictionary + - intervalsBed: + type: file + description: Bed file with the genomic regions included in the library (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - bam: + type: file + description: Recalibrated BAM file + pattern: "*.{bam}" + +authors: + - "@yocra3" diff --git a/modules/nf-core/software/gatk4/baserecalibrator/main.nf b/modules/nf-core/software/gatk4/baserecalibrator/main.nf index 31ffbd20..f13b7867 100644 --- a/modules/nf-core/software/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/software/gatk4/baserecalibrator/main.nf @@ -24,22 +24,28 @@ process GATK4_BASERECALIBRATOR { path fastaidx path dict path intervalsBed + path dbsnp + path dbsnp_index path knownSites - path knownSites_tbi + path knownSites_index + //output: + //tuple val(meta), path("*.table"), emit: table output: - tuple val(meta), path("*.table"), emit: table + path "*.table" , emit: table path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + def dbsnpOptions = dbsnp ? "--known-sites ${dbsnp}" : "" def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') """ gatk BaseRecalibrator \ -R $fasta \ -I $bam \ + $dbsnpOptions \ $sitesCommand \ $intervalsCommand \ $options.args \ diff --git a/modules/nf-core/software/gatk4/haplotypecaller/functions.nf b/modules/nf-core/software/gatk4/haplotypecaller/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/haplotypecaller/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/haplotypecaller/main.nf b/modules/nf-core/software/gatk4/haplotypecaller/main.nf new file mode 100644 index 00000000..638cf09e --- /dev/null +++ b/modules/nf-core/software/gatk4/haplotypecaller/main.nf @@ -0,0 +1,54 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_HAPLOTYPECALLER { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam) + path fasta + path fai + path dict + path intervalsBed + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk \\ + --java-options "-Xmx${avail_mem}g" \\ + HaplotypeCaller \\ + -R $fasta \\ + -I $bam \\ + -O ${prefix}.vcf.gz \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/haplotypecaller/meta.yml b/modules/nf-core/software/gatk4/haplotypecaller/meta.yml new file mode 100644 index 00000000..f0fc3910 --- /dev/null +++ b/modules/nf-core/software/gatk4/haplotypecaller/meta.yml @@ -0,0 +1,63 @@ +name: gatk4_haplotypecaller +description: Call germline SNPs and indels via local re-assembly of haplotypes +keywords: + - gatk4 + - haplotypecaller + - haplotype +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.bam" + - bai: + type: file + description: Index of BAM file + pattern: "*.bam.bai" + - fasta: + type: file + description: The reference fasta file + pattern: "*.fasta" + - fai: + type: file + description: Index of reference fasta file + pattern: "fasta.fai" + - dict: + type: file + description: GATK sequence dictionary + pattern: "*.dict" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - vcf: + type: file + description: Compressed VCF file + pattern: "*.vcf.gz" + - tbi: + type: file + description: Index of VCF file + pattern: "*.vcf.gz.tbi" + +authors: + - "@suzannejin" diff --git a/modules/nf-core/software/gatk4/intervallisttools/functions.nf b/modules/nf-core/software/gatk4/intervallisttools/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/intervallisttools/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/intervallisttools/main.nf b/modules/nf-core/software/gatk4/intervallisttools/main.nf new file mode 100644 index 00000000..ec65e02c --- /dev/null +++ b/modules/nf-core/software/gatk4/intervallisttools/main.nf @@ -0,0 +1,53 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_INTERVALLISTTOOLS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--hdfd78af_1" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--hdfd78af_1" + } + + input: + tuple val(meta), path(interval_list) + + output: + tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + + mkdir ${prefix}_split + + gatk \\ + IntervalListTools \\ + -I ${interval_list} \\ + -O ${prefix}_split \\ + $options.args + + python3 < ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/intervallisttools/meta.yml b/modules/nf-core/software/gatk4/intervallisttools/meta.yml new file mode 100644 index 00000000..efe31da1 --- /dev/null +++ b/modules/nf-core/software/gatk4/intervallisttools/meta.yml @@ -0,0 +1,46 @@ +name: gatk4_intervallisttools + +description: Splits the interval list file into unique, equally-sized interval files and place it under a directory +keywords: + - sort + - bed + - interval list +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + + - interval_list: + type: file + description: Interval list file + pattern: "*.interval_list" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - interval_list: + type: file + description: Interval list files + pattern: "*.interval_list" + +authors: + - "@praveenraj2018" diff --git a/modules/nf-core/software/star/align/main.nf b/modules/nf-core/software/star/align/main.nf index e85ddb79..1760ff16 100644 --- a/modules/nf-core/software/star/align/main.nf +++ b/modules/nf-core/software/star/align/main.nf @@ -14,9 +14,9 @@ process STAR_ALIGN { // Note: 2.7X indices incompatible with AWS iGenomes. conda (params.enable_conda ? 'bioconda::star=2.6.1d' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/star:2.6.1d--0' + container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' } else { - container 'quay.io/biocontainers/star:2.6.1d--0' + container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' } input: @@ -41,7 +41,7 @@ process STAR_ALIGN { def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" + def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' 'PL:illumina'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix' 'PL:illumina'" def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' """ diff --git a/nextflow.config b/nextflow.config index 57534f06..53d8514a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -62,6 +62,12 @@ params { skip_alignment = false skip_splitncigar = false skip_basecalibrator = false + skip_applybqsr = false + skip_haplotypecaller = false + skip_intervallisttools = false + + // GATK parameters + scatter_count = 6 // QC skip_qc = false diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 431cf0d4..1f665877 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -117,11 +117,20 @@ workflow PREPARE_GENOME { // Index the genome fasta ch_fasta_fai = Channel.empty() - ch_fasta_fai = SAMTOOLS_FAIDX ( ch_fasta ).fai + if( params.fasta_fai){ + ch_fasta_fai = file(params.fasta_fai) + } else { + ch_fasta_fai = SAMTOOLS_FAIDX ( ch_fasta ).fai + } // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() - ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY ( ch_fasta ).dict + if( params.fasta_dict){ + ch_fasta_dict = file(params.fasta_dict) + } + else{ + ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY ( ch_fasta ).dict + } // // Create chromosome sizes file // diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 819e4073..0f3f8335 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -71,6 +71,9 @@ if (params.save_unaligned) { star_align_options.publish_files.put('fastq. def picard_markduplicates_samtools = modules['picard_markduplicates_samtools'] picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +def intervaltools_options = modules['gatk_intervallisttools'] +intervaltools_options.args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) + include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) @@ -80,6 +83,9 @@ include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) +include { GATK4_APPLYBQSR } from '../modules/nf-core/software/gatk4/applybqsr/main' addParams( options: modules['gatk_applybqsr']) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) +include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) workflow RNASEQ_VAR { @@ -193,7 +199,7 @@ workflow RNASEQ_VAR { ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) } - /* + ch_bqsr_table = Channel.empty() if(!params.skip_basecalibrator){ GATK4_BASERECALIBRATOR( ch_genome_bam.join(ch_genome_bam_index, by: [0]), @@ -201,7 +207,36 @@ workflow RNASEQ_VAR { PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + params.known_indels, + params.known_indels_index + ) + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + } + + if(!params.skip_applybqsr){ + GATK4_APPLYBQSR( + ch_genome_bam.join(ch_genome_bam_index, by: [0]), + ch_bqsr_table, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list + ) + ch_genome_bam = GATK4_APPLYBQSR.out.bam + } + + if(!params.skip_haplotypecaller){ + GATK4_HAPLOTYPECALLER( + ch_genome_bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list ) - }*/ + + } + } } \ No newline at end of file From 2fad85a68a5008bbf9a51fb19ca5632f27e0aa00 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Jun 2021 10:43:41 +0200 Subject: [PATCH 013/235] Changed version of nf to 21.04.0 in ci.yml --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index c14a4d69..61ad9ac6 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,7 +23,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', ''] + nxf_ver: ['21.04.0', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 From 93779866aa03aa8b8e57fd603ad2b92f6711c940 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 28 Jun 2021 10:27:45 +0200 Subject: [PATCH 014/235] Issue fix required for the HC to run on splitted interval files --- conf/modules.config | 26 ++ .../nf-core/software/gatk4/applybqsr/main.nf | 6 +- .../software/gatk4/haplotypecaller/main.nf | 44 +++ .../software/gatk4/intervallisttools/main.nf | 12 +- .../software/qualimap/bamqc/functions.nf | 68 +++++ .../nf-core/software/qualimap/bamqc/main.nf | 61 ++++ .../nf-core/software/qualimap/bamqc/meta.yml | 49 ++++ .../software/samtools/merge/functions.nf | 68 +++++ .../nf-core/software/samtools/merge/main.nf | 35 +++ .../nf-core/software/samtools/merge/meta.yml | 44 +++ nextflow.config | 2 + subworkflows/nf-core/recalibrate.nf | 71 +++++ workflows/rnavar.nf | 84 +++++- workflows/rnavar_bk.nf | 268 ++++++++++++++++++ 14 files changed, 815 insertions(+), 23 deletions(-) create mode 100644 modules/nf-core/software/qualimap/bamqc/functions.nf create mode 100644 modules/nf-core/software/qualimap/bamqc/main.nf create mode 100644 modules/nf-core/software/qualimap/bamqc/meta.yml create mode 100644 modules/nf-core/software/samtools/merge/functions.nf create mode 100644 modules/nf-core/software/samtools/merge/main.nf create mode 100644 modules/nf-core/software/samtools/merge/meta.yml create mode 100644 subworkflows/nf-core/recalibrate.nf create mode 100644 workflows/rnavar_bk.nf diff --git a/conf/modules.config b/conf/modules.config index 498b9acc..221a5b2b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -86,5 +86,31 @@ params { publish_files = [:] publish_dir = "${params.aligner}/interval_lists" } + // RECALIBRATE + 'applybqsr' { + suffix = '.recal' + publish_files = false + } + 'merge_bam_recalibrate' { + suffix = '.recal' + publish_by_meta = true + publish_files = ['bam':'recalibrated'] + publish_dir = 'preprocessing' + } + 'qualimap_bamqc_recalibrate' { + args = '--paint-chromosome-limits --genome-gc-distr HUMAN -skip-duplicated --skip-dup-mode 0 -outformat HTML' + publish_by_meta = true + publish_dir = 'reports/qualimap' + } + 'samtools_index_recalibrate' { + suffix = 'recal' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['recal.bam':'recalibrated', 'recal.bam.bai':'recalibrated'] + } + 'samtools_stats_recalibrate' { + publish_by_meta = true + publish_dir = 'reports/samtools_stats' + } } } diff --git a/modules/nf-core/software/gatk4/applybqsr/main.nf b/modules/nf-core/software/gatk4/applybqsr/main.nf index 078c5ad5..941c2c7d 100644 --- a/modules/nf-core/software/gatk4/applybqsr/main.nf +++ b/modules/nf-core/software/gatk4/applybqsr/main.nf @@ -19,13 +19,11 @@ process GATK4_APPLYBQSR { } input: - tuple val(meta), path(bam), path(bai) - path bqsr_table + tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervalsBed) path fasta path fastaidx path dict - path intervalsBed - + output: tuple val(meta), path("*.bam"), emit: bam path "*.version.txt" , emit: version diff --git a/modules/nf-core/software/gatk4/haplotypecaller/main.nf b/modules/nf-core/software/gatk4/haplotypecaller/main.nf index 638cf09e..80906b79 100644 --- a/modules/nf-core/software/gatk4/haplotypecaller/main.nf +++ b/modules/nf-core/software/gatk4/haplotypecaller/main.nf @@ -18,6 +18,48 @@ process GATK4_HAPLOTYPECALLER { container "quay.io/biocontainers/gatk4:4.2.0.0--0" } + input: + tuple val(meta), path(bam), path(bai), path(interval) + path dbsnp + path dbsnp_tbi + path dict + path fasta + path fai + val no_intervals + + output: + tuple val(meta), path("*.vcf"), emit: vcf + tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf + path "*.version.txt", emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + def intervalsOptions = no_intervals ? "" : "-L ${interval}" + def dbsnpOptions = dbsnp ? "--D ${dbsnp}" : "" + """ + gatk \\ + --java-options "-Xmx${avail_mem}g" \\ + HaplotypeCaller \\ + -R $fasta \\ + -I $bam \\ + ${intervalsOptions} \\ + ${dbsnpOptions} \\ + -O ${prefix}.vcf \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} + +/* + input: tuple val(meta), path(bam) path fasta @@ -52,3 +94,5 @@ process GATK4_HAPLOTYPECALLER { gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt """ } + +*/ diff --git a/modules/nf-core/software/gatk4/intervallisttools/main.nf b/modules/nf-core/software/gatk4/intervallisttools/main.nf index ec65e02c..77c3d886 100644 --- a/modules/nf-core/software/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/software/gatk4/intervallisttools/main.nf @@ -5,11 +5,11 @@ params.options = [:] options = initOptions(params.options) process GATK4_INTERVALLISTTOOLS { - tag "$meta.id" + tag "$interval_list" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['']) } conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -19,15 +19,15 @@ process GATK4_INTERVALLISTTOOLS { } input: - tuple val(meta), path(interval_list) + path interval_list output: - tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list - path "*.version.txt" , emit: version + path "*_split/*/*.interval_list" , emit: interval_list + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${interval_list}${options.suffix}" : "${interval_list}" """ mkdir ${prefix}_split diff --git a/modules/nf-core/software/qualimap/bamqc/functions.nf b/modules/nf-core/software/qualimap/bamqc/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/qualimap/bamqc/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/qualimap/bamqc/main.nf b/modules/nf-core/software/qualimap/bamqc/main.nf new file mode 100644 index 00000000..463cde4c --- /dev/null +++ b/modules/nf-core/software/qualimap/bamqc/main.nf @@ -0,0 +1,61 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process QUALIMAP_BAMQC { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::qualimap=2.2.2d" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1" + } else { + container "quay.io/biocontainers/qualimap:2.2.2d--1" + } + + input: + tuple val(meta), path(bam) + path gff + val use_gff + + output: + tuple val(meta), path("${prefix}"), emit: results + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + + def collect_pairs = meta.single_end ? '' : '--collect-overlap-pairs' + def memory = task.memory.toGiga() + "G" + def regions = use_gff ? "--gff $gff" : '' + + def strandedness = 'non-strand-specific' + if (meta.strandedness == 'forward') { + strandedness = 'strand-specific-forward' + } else if (meta.strandedness == 'reverse') { + strandedness = 'strand-specific-reverse' + } + """ + unset DISPLAY + mkdir tmp + export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp + qualimap \\ + --java-mem-size=$memory \\ + bamqc \\ + $options.args \\ + -bam $bam \\ + $regions \\ + -p $strandedness \\ + $collect_pairs \\ + -outdir $prefix \\ + -nt $task.cpus + + echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/qualimap/bamqc/meta.yml b/modules/nf-core/software/qualimap/bamqc/meta.yml new file mode 100644 index 00000000..3c608f31 --- /dev/null +++ b/modules/nf-core/software/qualimap/bamqc/meta.yml @@ -0,0 +1,49 @@ +name: qualimap_bamqc +description: Evaluate alignment data +keywords: + - quality control + - qc + - bam +tools: + - qualimap: + description: | + Qualimap 2 is a platform-independent application written in + Java and R that provides both a Graphical User Interface and + a command-line interface to facilitate the quality control of + alignment sequencing data and its derivatives like feature counts. + homepage: http://qualimap.bioinfo.cipf.es/ + documentation: http://qualimap.conesalab.org/doc_html/index.html + doi: 10.1093/bioinformatics/bts503 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.{bam}" + - gff: + type: file + description: Feature file with regions of interest + pattern: "*.{gff,gtf,bed}" + - use_gff: + type: boolean + description: Specifies if feature file should be used or not +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - results: + type: dir + description: Qualimap results dir + pattern: "*/*" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@phue" diff --git a/modules/nf-core/software/samtools/merge/functions.nf b/modules/nf-core/software/samtools/merge/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/samtools/merge/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/samtools/merge/main.nf b/modules/nf-core/software/samtools/merge/main.nf new file mode 100644 index 00000000..81b2828c --- /dev/null +++ b/modules/nf-core/software/samtools/merge/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_MERGE { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::samtools=1.12" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.12--hd5e65b6_0" + } else { + container "quay.io/biocontainers/samtools:1.12--hd5e65b6_0" + } + + input: + tuple val(meta), path(bams) + + output: + tuple val(meta), path("*merged.bam"), emit: merged_bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + samtools merge ${prefix}_merged.bam $bams + echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/samtools/merge/meta.yml b/modules/nf-core/software/samtools/merge/meta.yml new file mode 100644 index 00000000..9092f22e --- /dev/null +++ b/modules/nf-core/software/samtools/merge/meta.yml @@ -0,0 +1,44 @@ +name: samtools_merge +description: Merge BAM file +keywords: + - merge + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - merged_bam: + type: file + description: BAM file + pattern: "*.{bam}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@yuukiiwa " + - "@maxulysse" diff --git a/nextflow.config b/nextflow.config index 53d8514a..797205a6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -68,6 +68,8 @@ params { // GATK parameters scatter_count = 6 + no_intervals = false + skip_qc = null // QC skip_qc = false diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf new file mode 100644 index 00000000..943a550f --- /dev/null +++ b/subworkflows/nf-core/recalibrate.nf @@ -0,0 +1,71 @@ +/* +======================================================================================== + RECALIBRATE +======================================================================================== +*/ + +params.applybqsr_options = [:] +params.merge_bam_options = [:] +params.qualimap_bamqc_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/software/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) +include { QUALIMAP_BAMQC } from '../../modules/nf-core/software/qualimap/bamqc/main' addParams(options: params.qualimap_bamqc_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams(options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/software/samtools/merge/main' addParams(options: params.merge_bam_options) +include { SAMTOOLS_STATS } from '../../modules/nf-core/software/samtools/stats/main' addParams(options: params.samtools_stats_options) + +workflow RECALIBRATE { + take: + skip_bamqc // boolean: true/false + skip_samtools // boolean: true/false + bam // channel: [mandatory] bam + dict // channel: [mandatory] dict + fai // channel: [mandatory] fai + fasta // channel: [mandatory] fasta + intervals // channel: [mandatory] intervals + + main: + + bam_recalibrated_index = Channel.empty() + bam_recalibrated = Channel.empty() + bam_reports = Channel.empty() + + bam.combine(intervals).map{ meta, bam, bai, recal, intervals -> + new_meta = meta.clone() + new_meta.id = meta.id + [new_meta, bam, bai, recal, intervals] + }.set{bam_intervals} + + APPLYBQSR(bam_intervals, fasta, fai, dict) + + // STEP 4.5: MERGING AND INDEXING THE RECALIBRATED BAM FILES + if (params.no_intervals) { + bam_recalibrated = APPLYBQSR.out.bam + } else { + APPLYBQSR.out.bam.map{ meta, bam -> + meta.id = meta.id + [meta, bam] + }.groupTuple().set{bam_recalibrated_interval} + + SAMTOOLS_MERGE(bam_recalibrated_interval) + bam_recalibrated = SAMTOOLS_MERGE.out.merged_bam + + SAMTOOLS_INDEX(bam_recalibrated) + bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) + + qualimap_bamqc = Channel.empty() + samtools_stats = Channel.empty() + + if (!skip_samtools) { + SAMTOOLS_STATS(bam_recalibrated_index) + samtools_stats = SAMTOOLS_STATS.out.stats + } + bam_reports = samtools_stats.mix(qualimap_bamqc) + } + + emit: + bam = bam_recalibrated_index + qc = bam_reports +} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 0f3f8335..de679edc 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -83,7 +83,13 @@ include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) -include { GATK4_APPLYBQSR } from '../modules/nf-core/software/gatk4/applybqsr/main' addParams( options: modules['gatk_applybqsr']) +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( + applybqsr_options: modules['applybqsr'], + merge_bam_options: modules['merge_bam_recalibrate'], + qualimap_bamqc_options: modules['qualimap_bamqc_recalibrate'], + samtools_index_options: modules['samtools_index_recalibrate'], + samtools_stats_options: modules['samtools_stats_recalibrate'] +) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) @@ -182,7 +188,7 @@ workflow RNASEQ_VAR { } // MODULE: SplitNCigarReads from GATK4 // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). - + bam_splitncigar = Channel.empty() if(!params.skip_splitncigar){ GATK4_SPLITNCIGARREADS ( @@ -197,12 +203,14 @@ workflow RNASEQ_VAR { ch_genome_bam = GATK4_SPLITNCIGARREADS.out.bam ch_genome_bam_index = SAMTOOLS_INDEX.out.bai ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) + bam_splitncigar = ch_genome_bam.join(ch_genome_bam_index, by: [0]) } ch_bqsr_table = Channel.empty() + if(!params.skip_basecalibrator){ GATK4_BASERECALIBRATOR( - ch_genome_bam.join(ch_genome_bam_index, by: [0]), + bam_splitncigar, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, @@ -215,28 +223,78 @@ workflow RNASEQ_VAR { ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table } + bam_applybqsr = bam_splitncigar.combine(ch_bqsr_table) + bam_recalibrated = Channel.empty() + bam_recalibrated_qc = Channel.empty() + + if(!params.skip_applybqsr){ - GATK4_APPLYBQSR( - ch_genome_bam.join(ch_genome_bam_index, by: [0]), - ch_bqsr_table, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, + RECALIBRATE( + ('bamqc' in params.skip_qc), + ('samtools' in params.skip_qc), + bam_applybqsr, PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.fasta, ch_interval_list ) - ch_genome_bam = GATK4_APPLYBQSR.out.bam + + bam_recalibrated = RECALIBRATE.out.bam + bam_recalibrated_qc = RECALIBRATE.out.qc + + } + + ch_interval_list_split = Channel.empty() + + if(!params.skip_intervallisttools){ + GATK4_INTERVALLISTTOOLS( + ch_interval_list + ) + ch_interval_list_split = Channel.from(GATK4_INTERVALLISTTOOLS.out.interval_list) + } + else{ + ch_interval_list_split = ch_interval_list } + interval_flag = true + ch_interval_list_split.view() + + if(!params.skip_haplotypecaller){ + + haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) + + bam_recalibrated.combine(ch_interval_list_split).map{ meta, bam, bai, interval_list -> + [meta, bam, bai, interval_list] + //new_meta = meta.clone() + //new_meta.id = meta.id + "_" + interval_list.baseName + //[new_meta, bam, bai, interval_list] + } + .set{haplotypecaller_interval_bam} + + haplotypecaller_interval_bam.view() + + /* + GATK4_HAPLOTYPECALLER( - ch_genome_bam, + haplotypecaller_interval_bam, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - ch_interval_list + interval_flag ) - } + + + haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf.map{ meta,vcf -> + meta.id = meta.sample + [meta, vcf] + }.groupTuple() + + */ + } } } \ No newline at end of file diff --git a/workflows/rnavar_bk.nf b/workflows/rnavar_bk.nf new file mode 100644 index 00000000..d0922330 --- /dev/null +++ b/workflows/rnavar_bk.nf @@ -0,0 +1,268 @@ +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. + + +def valid_params = [ + aligners : ['star'], +] + +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) + +// Validate input parameters +WorkflowRnavar.initialise(params, log, valid_params) + +// Check input path parameters to see if they exist +checkPathParamList = [ + params.input, + params.fasta, + params.gtf, params.gff, params.gene_bed, + params.star_index +] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +// Check alignment parameters +def prepareToolIndices = [] +if (!params.skip_alignment) { prepareToolIndices << params.aligner } + +def modules = params.modules.clone() + +def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] +def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] + + +def star_genomegenerate_options = modules['star_genomegenerate'] +if (!params.save_reference) { star_genomegenerate_options['publish_files'] = false } + +def samtools_sort_genome_options = modules['samtools_sort_genome'] +def samtools_index_genome_options = modules['samtools_index_genome'] +samtools_index_genome_options.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +if (['star'].contains(params.aligner)) { + if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { + samtools_sort_genome_options.publish_files.put('bam','') + samtools_index_genome_options.publish_files.put('bai','') + samtools_index_genome_options.publish_files.put('csi','') + } +} else { + if (params.save_align_intermeds || params.skip_markduplicates) { + samtools_sort_genome_options.publish_files.put('bam','') + samtools_index_genome_options.publish_files.put('bai','') + samtools_index_genome_options.publish_files.put('csi','') + } +} + +def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type + +def cat_fastq_options = modules['cat_fastq'] +if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } + +def star_align_options = modules['star_align'] +star_align_options.args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +if (params.save_align_intermeds) { star_align_options.publish_files.put('bam','') } +if (params.save_unaligned) { star_align_options.publish_files.put('fastq.gz','unmapped') } + +def picard_markduplicates_samtools = modules['picard_markduplicates_samtools'] +picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' + +def intervaltools_options = modules['gatk_intervallisttools'] +intervaltools_options.args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) + +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/software/gatk4/bedtointervallist/main' addParams( options: [:]) +include { SAMTOOLS_INDEX } from '../modules/nf-core/software/samtools/index/main' addParams( options: samtools_index_genome_options ) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) +include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) +include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) +include { GATK4_APPLYBQSR } from '../modules/nf-core/software/gatk4/applybqsr/main' addParams( options: modules['gatk_applybqsr']) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) +include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) + +workflow RNASEQ_VAR { + + PREPARE_GENOME ( + prepareToolIndices, + biotype + ) + ch_genome_gtf = Channel.empty() + ch_genome_gtf = PREPARE_GENOME.out.gene_bed + ch_software_versions = Channel.empty() + ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) + + + INPUT_CHECK ( + ch_input + ) + .map { + meta, fastq -> + meta.id = meta.id.split('_')[0..-2].join('_') + [ meta, fastq ] } + .groupTuple(by: [0]) + .branch { + meta, fastq -> + single : fastq.size() == 1 + return [ meta, fastq.flatten() ] + multiple: fastq.size() > 1 + return [ meta, fastq.flatten() ] + } + .set { ch_fastq } + + // MODULE: Concatenate FastQ files from same sample if required + // + CAT_FASTQ ( + ch_fastq.multiple + ) + .mix(ch_fastq.single) + .set { ch_cat_fastq } + + // PREPARE THE INTERVAL LIST + ch_interval_list = Channel.empty() + GATK4_BEDTOINTERVALLIST( + ch_genome_gtf, + PREPARE_GENOME.out.dict + ) + ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list + ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) + // + // SUBWORKFLOW: Alignment with STAR + // + ch_genome_bam = Channel.empty() + ch_genome_bam_index = Channel.empty() + ch_samtools_stats = Channel.empty() + ch_samtools_flagstat = Channel.empty() + ch_samtools_idxstats = Channel.empty() + ch_star_multiqc = Channel.empty() + ch_aligner_pca_multiqc = Channel.empty() + ch_aligner_clustering_multiqc = Channel.empty() + + if (!params.skip_alignment && params.aligner == 'star') { + ALIGN_STAR ( + ch_cat_fastq, + PREPARE_GENOME.out.star_index, + PREPARE_GENOME.out.gtf + ) + ch_genome_bam = ALIGN_STAR.out.bam + ch_genome_bam_index = ALIGN_STAR.out.bai + ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript + ch_samtools_stats = ALIGN_STAR.out.stats + ch_samtools_flagstat = ALIGN_STAR.out.flagstat + ch_samtools_idxstats = ALIGN_STAR.out.idxstats + ch_star_multiqc = ALIGN_STAR.out.log_final + if (params.bam_csi_index) { + ch_genome_bam_index = ALIGN_STAR.out.csi + } + ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) + ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) + + // SUBWORKFLOW: Mark duplicates with Picard + ch_markduplicates_multiqc = Channel.empty() + if (!params.skip_alignment && !params.skip_markduplicates) { + MARK_DUPLICATES_PICARD ( + ch_genome_bam + ) + ch_genome_bam = MARK_DUPLICATES_PICARD.out.bam + ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.bai + ch_samtools_stats = MARK_DUPLICATES_PICARD.out.stats + ch_samtools_flagstat = MARK_DUPLICATES_PICARD.out.flagstat + ch_samtools_idxstats = MARK_DUPLICATES_PICARD.out.idxstats + ch_markduplicates_multiqc = MARK_DUPLICATES_PICARD.out.metrics + if (params.bam_csi_index) { + ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.csi + } + ch_software_versions = ch_software_versions.mix(MARK_DUPLICATES_PICARD.out.picard_version.first().ifEmpty(null)) + } + // MODULE: SplitNCigarReads from GATK4 + // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). + + if(!params.skip_splitncigar){ + + GATK4_SPLITNCIGARREADS ( + ch_genome_bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict + ) + + SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) + + ch_genome_bam = GATK4_SPLITNCIGARREADS.out.bam + ch_genome_bam_index = SAMTOOLS_INDEX.out.bai + ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) + } + + ch_bqsr_table = Channel.empty() + if(!params.skip_basecalibrator){ + GATK4_BASERECALIBRATOR( + ch_genome_bam.join(ch_genome_bam_index, by: [0]), + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + params.known_indels, + params.known_indels_index + ) + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + } + + if(!params.skip_applybqsr){ + GATK4_APPLYBQSR( + ch_genome_bam.join(ch_genome_bam_index, by: [0]), + ch_bqsr_table, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list + ) + ch_genome_bam = GATK4_APPLYBQSR.out.bam + ch_genome_bam_index = SAMTOOLS_INDEX (ch_genome_bam).out.bai + } + + if(!params.skip_intervallisttools){ + GATK4_INTERVALLISTTOOLS( + ch_interval_list + ) + ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list + } + else{ + ch_interval_list_split = ch_interval_list + } + + interval_flag = true + + + if(!params.skip_haplotypecaller){ + + ch_genome_bam.combine(ch_genome_bam_index).combine(ch_interval_list).set{haplotypecaller_interval_bam} + + GATK4_HAPLOTYPECALLER( + haplotypecaller_interval_bam, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + interval_flag + ) + + haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf.map{ meta,vcf -> + meta.id = meta.sample + [meta, vcf] + }.groupTuple() + + + + } + + } +} \ No newline at end of file From 5911f92cb41b4c4cabbd45e13b2327f3609e7bb9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 29 Jun 2021 06:52:36 +0200 Subject: [PATCH 015/235] Added HC with parallel using splitted intervals, Added last step variant filteration --- assets/samplesheet_valid2.csv | 3 + conf/modules.config | 13 ++++ .../software/gatk4/intervallisttools/main.nf | 2 +- .../software/gatk4/mergevcfs/functions.nf | 68 +++++++++++++++++++ .../nf-core/software/gatk4/mergevcfs/main.nf | 49 +++++++++++++ .../nf-core/software/gatk4/mergevcfs/meta.yml | 42 ++++++++++++ .../gatk4/variantfiltration/functions.nf | 68 +++++++++++++++++++ .../software/gatk4/variantfiltration/main.nf | 44 ++++++++++++ .../software/gatk4/variantfiltration/meta.yml | 47 +++++++++++++ nextflow.config | 7 ++ subworkflows/local/input_check.nf | 1 + workflows/rnavar.nf | 54 +++++++++++---- 12 files changed, 382 insertions(+), 16 deletions(-) create mode 100644 assets/samplesheet_valid2.csv create mode 100644 modules/nf-core/software/gatk4/mergevcfs/functions.nf create mode 100644 modules/nf-core/software/gatk4/mergevcfs/main.nf create mode 100644 modules/nf-core/software/gatk4/mergevcfs/meta.yml create mode 100644 modules/nf-core/software/gatk4/variantfiltration/functions.nf create mode 100644 modules/nf-core/software/gatk4/variantfiltration/main.nf create mode 100644 modules/nf-core/software/gatk4/variantfiltration/meta.yml diff --git a/assets/samplesheet_valid2.csv b/assets/samplesheet_valid2.csv new file mode 100644 index 00000000..955a7947 --- /dev/null +++ b/assets/samplesheet_valid2.csv @@ -0,0 +1,3 @@ +sample,fastq_1,fastq_2,strandedness +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/conf/modules.config b/conf/modules.config index 221a5b2b..43e827f4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -112,5 +112,18 @@ params { publish_by_meta = true publish_dir = 'reports/samtools_stats' } + 'gatk_mergevcfs' { + suffix = 'haplotypecaller' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] + } + 'gatk_variantfilter' { + args = '' + suffix = 'filtered' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'filtered', 'vcf.gz.tbi':'filtered'] + } } } diff --git a/modules/nf-core/software/gatk4/intervallisttools/main.nf b/modules/nf-core/software/gatk4/intervallisttools/main.nf index 77c3d886..4c6eb99d 100644 --- a/modules/nf-core/software/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/software/gatk4/intervallisttools/main.nf @@ -41,7 +41,7 @@ process GATK4_INTERVALLISTTOOLS { python3 < !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/mergevcfs/main.nf b/modules/nf-core/software/gatk4/mergevcfs/main.nf new file mode 100644 index 00000000..f64a298d --- /dev/null +++ b/modules/nf-core/software/gatk4/mergevcfs/main.nf @@ -0,0 +1,49 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_MERGEVCFS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + } else { + container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + } + + input: + tuple val(meta), path(vcfs) + path(ref_dict) + val use_ref_dict + + output: + tuple val(meta), path('*.vcf.gz') , emit: vcf + tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi') , emit: vcf_index + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + // Make list of VCFs to merge + def input = "" + for (vcf in vcfs) { + input += " I=${vcf}" + } + def ref = use_ref_dict ? "D=${ref_dict}" : "" + """ + gatk MergeVcfs \\ + $input \\ + O=${prefix}.merged.vcf.gz \\ + $ref \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/mergevcfs/meta.yml b/modules/nf-core/software/gatk4/mergevcfs/meta.yml new file mode 100644 index 00000000..14b28fa0 --- /dev/null +++ b/modules/nf-core/software/gatk4/mergevcfs/meta.yml @@ -0,0 +1,42 @@ +name: gatk4_mergevcfs +description: Merges several vcf files +keywords: + - vcf + - merge +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - vcf: + type: list + description: Two or more VCF files + pattern: "*.{vcf,vcf.gz}" + - ref_dict: + type: file + description: Optional Sequence Dictionary as input + pattern: "*.dict" + - use_ref_dict: + type: boolean + description: Specify whether or not to use a given reference dictionary +output: + - vcf: + type: file + description: merged vcf file + pattern: "*.vcf.gz" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/modules/nf-core/software/gatk4/variantfiltration/functions.nf b/modules/nf-core/software/gatk4/variantfiltration/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/software/gatk4/variantfiltration/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/software/gatk4/variantfiltration/main.nf b/modules/nf-core/software/gatk4/variantfiltration/main.nf new file mode 100644 index 00000000..28c2c44c --- /dev/null +++ b/modules/nf-core/software/gatk4/variantfiltration/main.nf @@ -0,0 +1,44 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process GATK4_VARIANTFILTRATION { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(vcf), path(tbi) + path fasta + path fai + path dict + + output: + tuple val(meta), path("*.vcf"), emit: vcf + path "*.version.txt" , emit: version + + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + gatk VariantFiltration \\ + -R $fasta \\ + -V $vcf \\ + -O ${prefix}.vcf \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/nf-core/software/gatk4/variantfiltration/meta.yml b/modules/nf-core/software/gatk4/variantfiltration/meta.yml new file mode 100644 index 00000000..d7f72582 --- /dev/null +++ b/modules/nf-core/software/gatk4/variantfiltration/meta.yml @@ -0,0 +1,47 @@ +name: gatk4_variantfiltration +description: Filter variants +keywords: + - vcf + - filter +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - vcf: + type: list + description: Input VCF file + pattern: "*.{vcf}" + - fasta: + type: file + description: Fasta file of reference genome + pattern: "*.fasta" + - fai: + type: file + description: Index of fasta file + pattern: "*.fasta.fai" + - dict: + type: file + description: Sequence dictionary of fastea file + pattern: "*.dict" +output: + - vcf: + type: file + description: filtered VCF file + pattern: "*.filtered.{vcf}" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/nextflow.config b/nextflow.config index 797205a6..c7245c0b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -65,12 +65,19 @@ params { skip_applybqsr = false skip_haplotypecaller = false skip_intervallisttools = false + skip_variantfiltration = false // GATK parameters scatter_count = 6 no_intervals = false skip_qc = null + //GATK variant filter parameters + window = 35 + cluster = 3 + fs_filter = 30.0 + qd_filter = 2.0 + // QC skip_qc = false skip_bigwig = false diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index f2618388..c7bfae95 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -24,6 +24,7 @@ workflow INPUT_CHECK { def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample + meta.sample = row.sample meta.single_end = row.single_end.toBoolean() meta.strandedness = row.strandedness diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index de679edc..091ca21a 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -74,6 +74,13 @@ picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleAr def intervaltools_options = modules['gatk_intervallisttools'] intervaltools_options.args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) + +def gatk_variant_filter_options = modules['gatk_variantfilter'] +if (params.window) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--window $params.window"]) } +if (params.cluster) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--cluster $params.cluster"]) } +if (params.fs_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) } +if (params.qd_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) } + include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) @@ -92,6 +99,8 @@ include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( ) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) +include { GATK4_MERGEVCFS } from '../modules/nf-core/software/gatk4/mergevcfs/main' addParams( options: modules['gatk_mergevcfs']) +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/software/gatk4/variantfiltration/main' addParams( options: gatk_variant_filter_options) workflow RNASEQ_VAR { @@ -250,32 +259,29 @@ workflow RNASEQ_VAR { GATK4_INTERVALLISTTOOLS( ch_interval_list ) - ch_interval_list_split = Channel.from(GATK4_INTERVALLISTTOOLS.out.interval_list) + ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.flatten() } else{ ch_interval_list_split = ch_interval_list } - interval_flag = true - ch_interval_list_split.view() + interval_flag = false + //ch_interval_list_split.view() + + haplotypecaller_vcf = Channel.empty() - if(!params.skip_haplotypecaller){ haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) bam_recalibrated.combine(ch_interval_list_split).map{ meta, bam, bai, interval_list -> [meta, bam, bai, interval_list] - //new_meta = meta.clone() - //new_meta.id = meta.id + "_" + interval_list.baseName - //[new_meta, bam, bai, interval_list] + new_meta = meta.clone() + new_meta.id = meta.id + "_" + interval_list.baseName + [new_meta, bam, bai, interval_list] } .set{haplotypecaller_interval_bam} - haplotypecaller_interval_bam.view() - - /* - GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, params.dbsnp_vcf, @@ -286,15 +292,33 @@ workflow RNASEQ_VAR { interval_flag ) - - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf.map{ meta,vcf -> meta.id = meta.sample [meta, vcf] }.groupTuple() - */ + use_ref_dict = true + GATK4_MERGEVCFS( + haplotypecaller_raw, + PREPARE_GENOME.out.dict, + use_ref_dict + ) + + haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf_index + } + + if(!params.skip_variantfiltration){ + + GATK4_VARIANTFILTRATION( + haplotypecaller_vcf, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict + ) + + filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf + + } - } } } \ No newline at end of file From b775bc3397fe41d1f4353588843252a52f5f3b72 Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Fri, 2 Jul 2021 11:49:59 +0200 Subject: [PATCH 016/235] Delete rnavar_bk.nf This is file is not required --- workflows/rnavar_bk.nf | 268 ----------------------------------------- 1 file changed, 268 deletions(-) delete mode 100644 workflows/rnavar_bk.nf diff --git a/workflows/rnavar_bk.nf b/workflows/rnavar_bk.nf deleted file mode 100644 index d0922330..00000000 --- a/workflows/rnavar_bk.nf +++ /dev/null @@ -1,268 +0,0 @@ -/* -======================================================================================== - IMPORT LOCAL MODULES/SUBWORKFLOWS -======================================================================================== -*/ - -// Don't overwrite global params.modules, create a copy instead and use that within the main script. - - -def valid_params = [ - aligners : ['star'], -] - -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) - -// Validate input parameters -WorkflowRnavar.initialise(params, log, valid_params) - -// Check input path parameters to see if they exist -checkPathParamList = [ - params.input, - params.fasta, - params.gtf, params.gff, params.gene_bed, - params.star_index -] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } - -// Check alignment parameters -def prepareToolIndices = [] -if (!params.skip_alignment) { prepareToolIndices << params.aligner } - -def modules = params.modules.clone() - -def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] -def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] - - -def star_genomegenerate_options = modules['star_genomegenerate'] -if (!params.save_reference) { star_genomegenerate_options['publish_files'] = false } - -def samtools_sort_genome_options = modules['samtools_sort_genome'] -def samtools_index_genome_options = modules['samtools_index_genome'] -samtools_index_genome_options.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -if (['star'].contains(params.aligner)) { - if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { - samtools_sort_genome_options.publish_files.put('bam','') - samtools_index_genome_options.publish_files.put('bai','') - samtools_index_genome_options.publish_files.put('csi','') - } -} else { - if (params.save_align_intermeds || params.skip_markduplicates) { - samtools_sort_genome_options.publish_files.put('bam','') - samtools_index_genome_options.publish_files.put('bai','') - samtools_index_genome_options.publish_files.put('csi','') - } -} - -def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type - -def cat_fastq_options = modules['cat_fastq'] -if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } - -def star_align_options = modules['star_align'] -star_align_options.args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' -if (params.save_align_intermeds) { star_align_options.publish_files.put('bam','') } -if (params.save_unaligned) { star_align_options.publish_files.put('fastq.gz','unmapped') } - -def picard_markduplicates_samtools = modules['picard_markduplicates_samtools'] -picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' - -def intervaltools_options = modules['gatk_intervallisttools'] -intervaltools_options.args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) - -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) -include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/software/gatk4/bedtointervallist/main' addParams( options: [:]) -include { SAMTOOLS_INDEX } from '../modules/nf-core/software/samtools/index/main' addParams( options: samtools_index_genome_options ) -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) -include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) -include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) -include { GATK4_APPLYBQSR } from '../modules/nf-core/software/gatk4/applybqsr/main' addParams( options: modules['gatk_applybqsr']) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) -include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) - -workflow RNASEQ_VAR { - - PREPARE_GENOME ( - prepareToolIndices, - biotype - ) - ch_genome_gtf = Channel.empty() - ch_genome_gtf = PREPARE_GENOME.out.gene_bed - ch_software_versions = Channel.empty() - ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) - - - INPUT_CHECK ( - ch_input - ) - .map { - meta, fastq -> - meta.id = meta.id.split('_')[0..-2].join('_') - [ meta, fastq ] } - .groupTuple(by: [0]) - .branch { - meta, fastq -> - single : fastq.size() == 1 - return [ meta, fastq.flatten() ] - multiple: fastq.size() > 1 - return [ meta, fastq.flatten() ] - } - .set { ch_fastq } - - // MODULE: Concatenate FastQ files from same sample if required - // - CAT_FASTQ ( - ch_fastq.multiple - ) - .mix(ch_fastq.single) - .set { ch_cat_fastq } - - // PREPARE THE INTERVAL LIST - ch_interval_list = Channel.empty() - GATK4_BEDTOINTERVALLIST( - ch_genome_gtf, - PREPARE_GENOME.out.dict - ) - ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list - ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) - // - // SUBWORKFLOW: Alignment with STAR - // - ch_genome_bam = Channel.empty() - ch_genome_bam_index = Channel.empty() - ch_samtools_stats = Channel.empty() - ch_samtools_flagstat = Channel.empty() - ch_samtools_idxstats = Channel.empty() - ch_star_multiqc = Channel.empty() - ch_aligner_pca_multiqc = Channel.empty() - ch_aligner_clustering_multiqc = Channel.empty() - - if (!params.skip_alignment && params.aligner == 'star') { - ALIGN_STAR ( - ch_cat_fastq, - PREPARE_GENOME.out.star_index, - PREPARE_GENOME.out.gtf - ) - ch_genome_bam = ALIGN_STAR.out.bam - ch_genome_bam_index = ALIGN_STAR.out.bai - ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript - ch_samtools_stats = ALIGN_STAR.out.stats - ch_samtools_flagstat = ALIGN_STAR.out.flagstat - ch_samtools_idxstats = ALIGN_STAR.out.idxstats - ch_star_multiqc = ALIGN_STAR.out.log_final - if (params.bam_csi_index) { - ch_genome_bam_index = ALIGN_STAR.out.csi - } - ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) - ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) - - // SUBWORKFLOW: Mark duplicates with Picard - ch_markduplicates_multiqc = Channel.empty() - if (!params.skip_alignment && !params.skip_markduplicates) { - MARK_DUPLICATES_PICARD ( - ch_genome_bam - ) - ch_genome_bam = MARK_DUPLICATES_PICARD.out.bam - ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.bai - ch_samtools_stats = MARK_DUPLICATES_PICARD.out.stats - ch_samtools_flagstat = MARK_DUPLICATES_PICARD.out.flagstat - ch_samtools_idxstats = MARK_DUPLICATES_PICARD.out.idxstats - ch_markduplicates_multiqc = MARK_DUPLICATES_PICARD.out.metrics - if (params.bam_csi_index) { - ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.csi - } - ch_software_versions = ch_software_versions.mix(MARK_DUPLICATES_PICARD.out.picard_version.first().ifEmpty(null)) - } - // MODULE: SplitNCigarReads from GATK4 - // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). - - if(!params.skip_splitncigar){ - - GATK4_SPLITNCIGARREADS ( - ch_genome_bam, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict - ) - - SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) - - ch_genome_bam = GATK4_SPLITNCIGARREADS.out.bam - ch_genome_bam_index = SAMTOOLS_INDEX.out.bai - ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) - } - - ch_bqsr_table = Channel.empty() - if(!params.skip_basecalibrator){ - GATK4_BASERECALIBRATOR( - ch_genome_bam.join(ch_genome_bam_index, by: [0]), - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - ch_interval_list, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - params.known_indels, - params.known_indels_index - ) - ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table - } - - if(!params.skip_applybqsr){ - GATK4_APPLYBQSR( - ch_genome_bam.join(ch_genome_bam_index, by: [0]), - ch_bqsr_table, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - ch_interval_list - ) - ch_genome_bam = GATK4_APPLYBQSR.out.bam - ch_genome_bam_index = SAMTOOLS_INDEX (ch_genome_bam).out.bai - } - - if(!params.skip_intervallisttools){ - GATK4_INTERVALLISTTOOLS( - ch_interval_list - ) - ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list - } - else{ - ch_interval_list_split = ch_interval_list - } - - interval_flag = true - - - if(!params.skip_haplotypecaller){ - - ch_genome_bam.combine(ch_genome_bam_index).combine(ch_interval_list).set{haplotypecaller_interval_bam} - - GATK4_HAPLOTYPECALLER( - haplotypecaller_interval_bam, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - interval_flag - ) - - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf.map{ meta,vcf -> - meta.id = meta.sample - [meta, vcf] - }.groupTuple() - - - - } - - } -} \ No newline at end of file From 37520d1241b661c0e090a6bbf0dacb640224efec Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 2 Jul 2021 15:36:19 +0200 Subject: [PATCH 017/235] Fixed issues when tested for multiple samples --- assets/samplesheet_valid_mixed.csv | 6 ++++++ conf/modules.config | 2 +- modules/nf-core/software/gatk4/baserecalibrator/main.nf | 4 ++-- modules/nf-core/software/gatk4/variantfiltration/main.nf | 7 ++++--- workflows/rnavar.nf | 4 ++-- 5 files changed, 15 insertions(+), 8 deletions(-) create mode 100644 assets/samplesheet_valid_mixed.csv diff --git a/assets/samplesheet_valid_mixed.csv b/assets/samplesheet_valid_mixed.csv new file mode 100644 index 00000000..ae5a54c7 --- /dev/null +++ b/assets/samplesheet_valid_mixed.csv @@ -0,0 +1,6 @@ +sample,fastq_1,fastq_2,strandedness +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +SE12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,,reverse +TUMOR1,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz,reverse +TUMOR2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz,reverse diff --git a/conf/modules.config b/conf/modules.config index 43e827f4..c505140d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -123,7 +123,7 @@ params { suffix = 'filtered' publish_by_meta = true publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'filtered', 'vcf.gz.tbi':'filtered'] + publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] } } } diff --git a/modules/nf-core/software/gatk4/baserecalibrator/main.nf b/modules/nf-core/software/gatk4/baserecalibrator/main.nf index f13b7867..4ba1e78e 100644 --- a/modules/nf-core/software/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/software/gatk4/baserecalibrator/main.nf @@ -32,8 +32,8 @@ process GATK4_BASERECALIBRATOR { //output: //tuple val(meta), path("*.table"), emit: table output: - path "*.table" , emit: table - path "*.version.txt" , emit: version + tuple val(meta), path("*.table") , emit: table + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) diff --git a/modules/nf-core/software/gatk4/variantfiltration/main.nf b/modules/nf-core/software/gatk4/variantfiltration/main.nf index 28c2c44c..21c3c44f 100644 --- a/modules/nf-core/software/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/software/gatk4/variantfiltration/main.nf @@ -25,8 +25,9 @@ process GATK4_VARIANTFILTRATION { path dict output: - tuple val(meta), path("*.vcf"), emit: vcf - path "*.version.txt" , emit: version + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi') , emit: vcf_index + path "*.version.txt" , emit: version script: @@ -36,7 +37,7 @@ process GATK4_VARIANTFILTRATION { gatk VariantFiltration \\ -R $fasta \\ -V $vcf \\ - -O ${prefix}.vcf \\ + -O ${prefix}.vcf.gz \\ $options.args gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 091ca21a..4521ed41 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -232,7 +232,8 @@ workflow RNASEQ_VAR { ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table } - bam_applybqsr = bam_splitncigar.combine(ch_bqsr_table) + bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) + bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() @@ -266,7 +267,6 @@ workflow RNASEQ_VAR { } interval_flag = false - //ch_interval_list_split.view() haplotypecaller_vcf = Channel.empty() From 7a7787e650d7c7fac0ce86f3ce295053333902a1 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 2 Jul 2021 15:40:32 +0200 Subject: [PATCH 018/235] Fixed issues when tested for multiple samples --- modules/nf-core/software/gatk4/variantfiltration/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/software/gatk4/variantfiltration/main.nf b/modules/nf-core/software/gatk4/variantfiltration/main.nf index 21c3c44f..e8bf047c 100644 --- a/modules/nf-core/software/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/software/gatk4/variantfiltration/main.nf @@ -32,7 +32,7 @@ process GATK4_VARIANTFILTRATION { script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" """ gatk VariantFiltration \\ -R $fasta \\ From fb031e0e890bbc71240600acf2c57130c418bf6c Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 5 Jul 2021 11:58:01 +0200 Subject: [PATCH 019/235] minor edits --- assets/samplesheet_valid_mixed.csv | 3 --- main.nf | 9 ++++----- nextflow.config | 4 ++-- 3 files changed, 6 insertions(+), 10 deletions(-) diff --git a/assets/samplesheet_valid_mixed.csv b/assets/samplesheet_valid_mixed.csv index ae5a54c7..955a7947 100644 --- a/assets/samplesheet_valid_mixed.csv +++ b/assets/samplesheet_valid_mixed.csv @@ -1,6 +1,3 @@ sample,fastq_1,fastq_2,strandedness GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -SE12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,,reverse -TUMOR1,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz,reverse -TUMOR2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz,reverse diff --git a/main.nf b/main.nf index be192e6c..87f32db0 100644 --- a/main.nf +++ b/main.nf @@ -1,11 +1,11 @@ #!/usr/bin/env nextflow /* ======================================================================================== - nf-core/rnavar +nf-core/rnavar ======================================================================================== - nf-core/rnavar Analysis Pipeline. - #### Homepage / Documentation - https://github.com/nf-core/rnavar +nf-core/rnavar Analysis Pipeline. +#### Homepage / Documentation +https://github.com/nf-core/rnavar ---------------------------------------------------------------------------------------- */ @@ -41,7 +41,6 @@ RUN THE WORKFLOW workflow { include { RNASEQ_VAR } from './workflows/rnavar' RNASEQ_VAR () - } /* diff --git a/nextflow.config b/nextflow.config index c7245c0b..97299153 100644 --- a/nextflow.config +++ b/nextflow.config @@ -237,8 +237,8 @@ dag { } manifest { - name = 'nf-core/rnaseq' - author = 'Phil Ewels, Rickard Hammarén' + name = 'nf-core/rnavar' + author = 'Praveen Raj' homePage = 'https://github.com/nf-core/rnaseq' description = 'Nextflow RNA-Seq analysis pipeline, part of the nf-core community.' mainScript = 'main.nf' From 07bf04dc564cab1b1beb65e1504b39882897f2d9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 6 Jul 2021 09:43:30 +0200 Subject: [PATCH 020/235] fix: lint errors fixed --- .nf-core-lint.yml | 8 ++ Dockerfile | 4 +- LICENSE | 2 +- README.md | 4 +- assets/email_template.html | 2 +- backup/nextflow.config | 192 ------------------------------------- environment.yml | 14 +-- nextflow_schema.json | 76 ++++++++++++++- 8 files changed, 95 insertions(+), 207 deletions(-) create mode 100644 .nf-core-lint.yml delete mode 100644 backup/nextflow.config diff --git a/.nf-core-lint.yml b/.nf-core-lint.yml new file mode 100644 index 00000000..f50d6f04 --- /dev/null +++ b/.nf-core-lint.yml @@ -0,0 +1,8 @@ +files_unchanged: + - .github/ISSUE_TEMPLATE/bug_report.md + - .github/ISSUE_TEMPLATE/feature_request.md + - .github/PULL_REQUEST_TEMPLATE.md + - .gitignore + - assets/nf-core-rnavar_logo.png + - docs/images/nf-core-rnavar_logo.png + - lib/NfcoreSchema.groovy diff --git a/Dockerfile b/Dockerfile index 8cb51485..850160a3 100644 --- a/Dockerfile +++ b/Dockerfile @@ -7,7 +7,7 @@ COPY environment.yml / RUN conda env create --quiet -f /environment.yml && conda clean -a # Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-rnavar-1.0dev/bin:$PATH +ENV PATH /opt/conda/envs/nf-core-rnavar-3.1/bin:$PATH # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-rnavar-1.0dev > nf-core-rnavar-1.0dev.yml +RUN conda env export --name nf-core-rnavar-3.1 > nf-core-rnavar-3.1.yml diff --git a/LICENSE b/LICENSE index fa54ba9b..bc2712ac 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Maxime U Garcia +Copyright (c) Praveen Raj Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index aa4b7b8d..c40dc704 100644 --- a/README.md +++ b/README.md @@ -4,7 +4,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions) [![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions) -[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A520.04.0-brightgreen.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A521.04.0-brightgreen.svg)](https://www.nextflow.io/) [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/) [![Docker](https://img.shields.io/docker/automated/nfcore/rnavar.svg)](https://hub.docker.com/r/nfcore/rnavar) @@ -19,7 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ## Quick Start -1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`) +1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=21.04.0`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ diff --git a/assets/email_template.html b/assets/email_template.html index c2b2eb54..1b6ecc5f 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/rnavar Pipeline Report diff --git a/backup/nextflow.config b/backup/nextflow.config deleted file mode 100644 index e268676e..00000000 --- a/backup/nextflow.config +++ /dev/null @@ -1,192 +0,0 @@ -/* - * ------------------------------------------------- - * nf-core/rnavar Nextflow config file - * ------------------------------------------------- - * Default config options for all environments. - */ - -// Global default params, used in configs -params { - - // Workflow flags - // TODO nf-core: Specify your pipeline's command line flags - genome = false - input = null - input_paths = null - single_end = false - outdir = './results' - publish_dir_mode = 'copy' - - // Boilerplate options - multiqc_config = false - email = false - email_on_fail = false - max_multiqc_email_size = 25.MB - plaintext_email = false - monochrome_logs = false - help = false - igenomes_base = 's3://ngi-igenomes/igenomes' - tracedir = "${params.outdir}/pipeline_info" - igenomes_ignore = false - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = false - config_profile_name = null - config_profile_description = false - config_profile_contact = false - config_profile_url = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes,input_paths' - - // Defaults only, expecting to be overwritten - max_memory = 128.GB - max_cpus = 16 - max_time = 240.h - -} - -// Container slug. Stable releases should specify release tag! -// Developmental code should specify :dev -process.container = 'nfcore/rnavar:dev' - -// Load base.config by default for all pipelines -includeConfig 'conf/base.config' - -// Load nf-core custom profiles from different Institutions -try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -profiles { - conda { - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - process.conda = "$projectDir/environment.yml" - } - debug { process.beforeScript = 'echo $HOSTNAME' } - docker { - docker.enabled = true - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - // Avoid this error: - // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. - // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 - // once this is established and works well, nextflow might implement this behavior as new default. - docker.runOptions = '-u \$(id -u):\$(id -g)' - } - singularity { - docker.enabled = false - singularity.enabled = true - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - singularity.autoMounts = true - } - podman { - singularity.enabled = false - docker.enabled = false - podman.enabled = true - shifter.enabled = false - charliecloud.enabled = false - } - shifter { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = true - charliecloud.enabled = false - } - charliecloud { - singularity.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = true - } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } -} - -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} - -// Export these variables to prevent local Python/R libraries from conflicting with those in the container -env { - PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" -} - -// Capture exit codes from upstream processes when piping -process.shell = ['/bin/bash', '-euo', 'pipefail'] - -def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') -timeline { - enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" -} -report { - enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" -} -trace { - enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" -} -dag { - enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" -} - -manifest { - name = 'nf-core/rnavar' - author = 'Maxime U Garcia' - homePage = 'https://github.com/nf-core/rnavar' - description = 'gatk4 rna variant calling' - mainScript = 'main.nf' - nextflowVersion = '>=20.04.0' - version = '1.0dev' -} - -// Function to ensure that resource requirements don't go beyond -// a maximum limit -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } - } -} diff --git a/environment.yml b/environment.yml index 5c3e7f4e..7e1753f8 100644 --- a/environment.yml +++ b/environment.yml @@ -1,15 +1,15 @@ # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml -name: nf-core-rnavar-1.0dev +name: nf-core-rnavar-3.1 channels: - conda-forge - bioconda - defaults dependencies: - - conda-forge::python=3.7.3 - - conda-forge::markdown=3.1.1 - - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.5.2 + - conda-forge::python=3.9.5 + - conda-forge::markdown=3.3.4 + - conda-forge::pymdown-extensions=8.2 + - conda-forge::pygments=2.9.0 # TODO nf-core: Add required software dependencies here - - bioconda::fastqc=0.11.8 - - bioconda::multiqc=1.7 + - bioconda::fastqc=0.11.9 + - bioconda::multiqc=1.11 diff --git a/nextflow_schema.json b/nextflow_schema.json index 750f3148..a079e988 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,6 +1,6 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/rnaseq/master/nextflow_schema.json", + "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/nextflow_schema.json", "title": "nf-core/rnaseq pipeline parameters", "description": "Nextflow RNA-Seq analysis pipeline, part of the nf-core community.", "type": "object", @@ -399,6 +399,44 @@ } } }, + "analysis_options": { + "title": "Analysis options", + "type": "object", + "fa_icon": "fas fa-fast-forward", + "description": "Module parameters of GATK.", + "properties": { + "scatter_count": { + "type": "integer", + "fa_icon": "fas fa-fast-forward", + "description": "Set the scatter count for the intervalListTools. This parameter defines how many chunks to prepare from the bed file. Default 6." + }, + "no_intervals": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Flag to set the presence or absence of an Interval bed file. Default true." + }, + "window": { + "type": "integer", + "fa_icon": "fas fa-fast-forward", + "description": "GATK Variant filtering parameter -window. The window size (in bases) in which to evaluate clustered SNPs. Default is 35." + }, + "cluster": { + "type": "integer", + "fa_icon": "fas fa-fast-forward", + "description": "GATK Variant filtering parameter -cluster. The number of SNPs which make up a cluster. Default is 3." + }, + "fs_filter": { + "type": "number", + "fa_icon": "fas fa-fast-forward", + "description": "GATK Variant filtering parameter - FisherStrand (FS). Default is FS > 30.0" + }, + "qd_filter": { + "type": "number", + "fa_icon": "fas fa-fast-forward", + "description": "GATK Variant filtering parameter - Quality-by-Depth. Default is QD < 2.0" + } + } + }, "process_skipping_options": { "title": "Process skipping options", "type": "object", @@ -471,7 +509,38 @@ "type": "boolean", "fa_icon": "fas fa-fast-forward", "description": "Skip all QC steps except for MultiQC." - } + }, + "skip_splitncigar": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip SplitNCigar module of GATK." + }, + "skip_basecalibrator": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip GATK base calibration step." + }, + "skip_applybqsr": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip GATK applying base calibration step." + }, + "skip_haplotypecaller": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip GATK variant calling." + }, + "skip_intervallisttools": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip splitting of interval BED file into chucks for parallel threads." + }, + "skip_variantfiltration": { + "type": "boolean", + "fa_icon": "fas fa-fast-forward", + "description": "Skip variant filtering step." + } + } }, "institutional_config_options": { @@ -672,6 +741,9 @@ { "$ref": "#/definitions/read_filtering_options" }, + { + "$ref": "#/definitions/analysis_options" + }, { "$ref": "#/definitions/reference_genome_options" }, From d5a70b63175e981205b95bd40dbeaf2a9644d2a8 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 15:58:07 +0200 Subject: [PATCH 021/235] fix: EClint --- nextflow_schema.json | 2 +- subworkflows/local/prepare_genome.nf | 16 +++++----------- 2 files changed, 6 insertions(+), 12 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 28f18263..f4a0adaa 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -408,4 +408,4 @@ "type": "string" } } -} \ No newline at end of file +} diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 1f665877..ae280892 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -117,20 +117,14 @@ workflow PREPARE_GENOME { // Index the genome fasta ch_fasta_fai = Channel.empty() - if( params.fasta_fai){ - ch_fasta_fai = file(params.fasta_fai) - } else { - ch_fasta_fai = SAMTOOLS_FAIDX ( ch_fasta ).fai - } + if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) + else ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() - if( params.fasta_dict){ - ch_fasta_dict = file(params.fasta_dict) - } - else{ - ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY ( ch_fasta ).dict - } + if (params.fasta_dict) ch_fasta_dict = file(params.fasta_dict) + else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict + // // Create chromosome sizes file // From 13f971d7286edae56eb86651729294a0aab6d8e4 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 16:03:41 +0200 Subject: [PATCH 022/235] move files --- .nf-core-lint.yml => .nf-core.yml | 0 modules/nf-core/{software/cat => modules}/fastq/functions.nf | 0 modules/nf-core/{software/cat => modules}/fastq/main.nf | 0 modules/nf-core/{software/cat => modules}/fastq/meta.yml | 0 .../nf-core/{software => modules}/gatk4/applybqsr/functions.nf | 0 modules/nf-core/{software => modules}/gatk4/applybqsr/main.nf | 0 modules/nf-core/{software => modules}/gatk4/applybqsr/meta.yml | 0 .../{software => modules}/gatk4/baserecalibrator/functions.nf | 0 .../nf-core/{software => modules}/gatk4/baserecalibrator/main.nf | 0 .../nf-core/{software => modules}/gatk4/baserecalibrator/meta.yml | 0 .../{software => modules}/gatk4/bedtointervallist/functions.nf | 0 .../nf-core/{software => modules}/gatk4/bedtointervallist/main.nf | 0 .../{software => modules}/gatk4/bedtointervallist/meta.yml | 0 .../gatk4/createsequencedictionary/functions.nf | 0 .../{software => modules}/gatk4/createsequencedictionary/main.nf | 0 .../{software => 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b/modules/nf-core/modules/fastq/meta.yml similarity index 100% rename from modules/nf-core/software/cat/fastq/meta.yml rename to modules/nf-core/modules/fastq/meta.yml diff --git a/modules/nf-core/software/gatk4/applybqsr/functions.nf b/modules/nf-core/modules/gatk4/applybqsr/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/applybqsr/functions.nf rename to modules/nf-core/modules/gatk4/applybqsr/functions.nf diff --git a/modules/nf-core/software/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/applybqsr/main.nf rename to modules/nf-core/modules/gatk4/applybqsr/main.nf diff --git a/modules/nf-core/software/gatk4/applybqsr/meta.yml b/modules/nf-core/modules/gatk4/applybqsr/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/applybqsr/meta.yml rename to modules/nf-core/modules/gatk4/applybqsr/meta.yml diff --git a/modules/nf-core/software/gatk4/baserecalibrator/functions.nf b/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/baserecalibrator/functions.nf rename to modules/nf-core/modules/gatk4/baserecalibrator/functions.nf diff --git a/modules/nf-core/software/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/baserecalibrator/main.nf rename to modules/nf-core/modules/gatk4/baserecalibrator/main.nf diff --git a/modules/nf-core/software/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/baserecalibrator/meta.yml rename to modules/nf-core/modules/gatk4/baserecalibrator/meta.yml diff --git a/modules/nf-core/software/gatk4/bedtointervallist/functions.nf b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/bedtointervallist/functions.nf rename to modules/nf-core/modules/gatk4/bedtointervallist/functions.nf diff --git a/modules/nf-core/software/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/bedtointervallist/main.nf rename to modules/nf-core/modules/gatk4/bedtointervallist/main.nf diff --git a/modules/nf-core/software/gatk4/bedtointervallist/meta.yml b/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/bedtointervallist/meta.yml rename to modules/nf-core/modules/gatk4/bedtointervallist/meta.yml diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/functions.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/createsequencedictionary/functions.nf rename to modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/createsequencedictionary/main.nf rename to modules/nf-core/modules/gatk4/createsequencedictionary/main.nf diff --git a/modules/nf-core/software/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/createsequencedictionary/meta.yml rename to modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml diff --git a/modules/nf-core/software/gatk4/haplotypecaller/functions.nf b/modules/nf-core/modules/gatk4/haplotypecaller/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/haplotypecaller/functions.nf rename to modules/nf-core/modules/gatk4/haplotypecaller/functions.nf diff --git a/modules/nf-core/software/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/haplotypecaller/main.nf rename to modules/nf-core/modules/gatk4/haplotypecaller/main.nf diff --git a/modules/nf-core/software/gatk4/haplotypecaller/meta.yml b/modules/nf-core/modules/gatk4/haplotypecaller/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/haplotypecaller/meta.yml rename to modules/nf-core/modules/gatk4/haplotypecaller/meta.yml diff --git a/modules/nf-core/software/gatk4/intervallisttools/functions.nf b/modules/nf-core/modules/gatk4/intervallisttools/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/intervallisttools/functions.nf rename to modules/nf-core/modules/gatk4/intervallisttools/functions.nf diff --git a/modules/nf-core/software/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/intervallisttools/main.nf rename to modules/nf-core/modules/gatk4/intervallisttools/main.nf diff --git a/modules/nf-core/software/gatk4/intervallisttools/meta.yml b/modules/nf-core/modules/gatk4/intervallisttools/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/intervallisttools/meta.yml rename to modules/nf-core/modules/gatk4/intervallisttools/meta.yml diff --git a/modules/nf-core/software/gatk4/mergevcfs/functions.nf b/modules/nf-core/modules/gatk4/mergevcfs/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/mergevcfs/functions.nf rename to modules/nf-core/modules/gatk4/mergevcfs/functions.nf diff --git a/modules/nf-core/software/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/mergevcfs/main.nf rename to 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b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml similarity index 100% rename from modules/nf-core/software/gatk4/splitncigarreads/meta.yml rename to modules/nf-core/modules/gatk4/splitncigarreads/meta.yml diff --git a/modules/nf-core/software/gatk4/variantfiltration/functions.nf b/modules/nf-core/modules/gatk4/variantfiltration/functions.nf similarity index 100% rename from modules/nf-core/software/gatk4/variantfiltration/functions.nf rename to modules/nf-core/modules/gatk4/variantfiltration/functions.nf diff --git a/modules/nf-core/software/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf similarity index 100% rename from modules/nf-core/software/gatk4/variantfiltration/main.nf rename to modules/nf-core/modules/gatk4/variantfiltration/main.nf diff --git a/modules/nf-core/software/gatk4/variantfiltration/meta.yml b/modules/nf-core/modules/gatk4/variantfiltration/meta.yml similarity index 100% rename from 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rename to modules/nf-core/modules/gunzip/functions.nf diff --git a/modules/nf-core/software/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf similarity index 100% rename from modules/nf-core/software/gunzip/main.nf rename to modules/nf-core/modules/gunzip/main.nf diff --git a/modules/nf-core/software/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml similarity index 100% rename from modules/nf-core/software/gunzip/meta.yml rename to modules/nf-core/modules/gunzip/meta.yml diff --git a/modules/nf-core/software/hisat2/build/functions.nf b/modules/nf-core/modules/hisat2/build/functions.nf similarity index 100% rename from modules/nf-core/software/hisat2/build/functions.nf rename to modules/nf-core/modules/hisat2/build/functions.nf diff --git a/modules/nf-core/software/hisat2/build/main.nf b/modules/nf-core/modules/hisat2/build/main.nf similarity index 100% rename from modules/nf-core/software/hisat2/build/main.nf rename to modules/nf-core/modules/hisat2/build/main.nf diff --git a/modules/nf-core/software/hisat2/build/meta.yml b/modules/nf-core/modules/hisat2/build/meta.yml similarity index 100% rename from modules/nf-core/software/hisat2/build/meta.yml rename to modules/nf-core/modules/hisat2/build/meta.yml diff --git a/modules/nf-core/software/hisat2/extractsplicesites/functions.nf b/modules/nf-core/modules/hisat2/extractsplicesites/functions.nf similarity index 100% rename from modules/nf-core/software/hisat2/extractsplicesites/functions.nf rename to modules/nf-core/modules/hisat2/extractsplicesites/functions.nf diff --git a/modules/nf-core/software/hisat2/extractsplicesites/main.nf b/modules/nf-core/modules/hisat2/extractsplicesites/main.nf similarity index 100% rename from modules/nf-core/software/hisat2/extractsplicesites/main.nf rename to modules/nf-core/modules/hisat2/extractsplicesites/main.nf diff --git a/modules/nf-core/software/hisat2/extractsplicesites/meta.yml b/modules/nf-core/modules/hisat2/extractsplicesites/meta.yml similarity index 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diff --git a/modules/nf-core/software/qualimap/bamqc/functions.nf b/modules/nf-core/modules/qualimap/bamqc/functions.nf similarity index 100% rename from modules/nf-core/software/qualimap/bamqc/functions.nf rename to modules/nf-core/modules/qualimap/bamqc/functions.nf diff --git a/modules/nf-core/software/qualimap/bamqc/main.nf b/modules/nf-core/modules/qualimap/bamqc/main.nf similarity index 100% rename from modules/nf-core/software/qualimap/bamqc/main.nf rename to modules/nf-core/modules/qualimap/bamqc/main.nf diff --git a/modules/nf-core/software/qualimap/bamqc/meta.yml b/modules/nf-core/modules/qualimap/bamqc/meta.yml similarity index 100% rename from modules/nf-core/software/qualimap/bamqc/meta.yml rename to modules/nf-core/modules/qualimap/bamqc/meta.yml diff --git a/modules/nf-core/software/rsem/preparereference/functions.nf b/modules/nf-core/modules/rsem/preparereference/functions.nf similarity index 100% rename from modules/nf-core/software/rsem/preparereference/functions.nf rename to modules/nf-core/modules/rsem/preparereference/functions.nf diff --git a/modules/nf-core/software/rsem/preparereference/main.nf b/modules/nf-core/modules/rsem/preparereference/main.nf similarity index 100% rename from modules/nf-core/software/rsem/preparereference/main.nf rename to modules/nf-core/modules/rsem/preparereference/main.nf diff --git a/modules/nf-core/software/salmon/index/functions.nf b/modules/nf-core/modules/salmon/index/functions.nf similarity index 100% rename from modules/nf-core/software/salmon/index/functions.nf rename to modules/nf-core/modules/salmon/index/functions.nf diff --git a/modules/nf-core/software/salmon/index/main.nf b/modules/nf-core/modules/salmon/index/main.nf similarity index 100% rename from modules/nf-core/software/salmon/index/main.nf rename to modules/nf-core/modules/salmon/index/main.nf diff --git a/modules/nf-core/software/salmon/index/meta.yml b/modules/nf-core/modules/salmon/index/meta.yml similarity index 100% rename from modules/nf-core/software/salmon/index/meta.yml rename to modules/nf-core/modules/salmon/index/meta.yml diff --git a/modules/nf-core/software/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/faidx/functions.nf rename to modules/nf-core/modules/samtools/faidx/functions.nf diff --git a/modules/nf-core/software/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf similarity index 100% rename from modules/nf-core/software/samtools/faidx/main.nf rename to modules/nf-core/modules/samtools/faidx/main.nf diff --git a/modules/nf-core/software/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml similarity index 100% rename from modules/nf-core/software/samtools/faidx/meta.yml rename to modules/nf-core/modules/samtools/faidx/meta.yml diff --git a/modules/nf-core/software/samtools/flagstat/functions.nf b/modules/nf-core/modules/samtools/flagstat/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/flagstat/functions.nf rename to modules/nf-core/modules/samtools/flagstat/functions.nf diff --git a/modules/nf-core/software/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf similarity index 100% rename from modules/nf-core/software/samtools/flagstat/main.nf rename to modules/nf-core/modules/samtools/flagstat/main.nf diff --git a/modules/nf-core/software/samtools/flagstat/meta.yml b/modules/nf-core/modules/samtools/flagstat/meta.yml similarity index 100% rename from modules/nf-core/software/samtools/flagstat/meta.yml rename to modules/nf-core/modules/samtools/flagstat/meta.yml diff --git a/modules/nf-core/software/samtools/idxstats/functions.nf b/modules/nf-core/modules/samtools/idxstats/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/idxstats/functions.nf rename to modules/nf-core/modules/samtools/idxstats/functions.nf diff --git a/modules/nf-core/software/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf similarity index 100% rename from modules/nf-core/software/samtools/idxstats/main.nf rename to modules/nf-core/modules/samtools/idxstats/main.nf diff --git a/modules/nf-core/software/samtools/idxstats/meta.yml b/modules/nf-core/modules/samtools/idxstats/meta.yml similarity index 100% rename from modules/nf-core/software/samtools/idxstats/meta.yml rename to modules/nf-core/modules/samtools/idxstats/meta.yml diff --git a/modules/nf-core/software/samtools/index/functions.nf b/modules/nf-core/modules/samtools/index/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/index/functions.nf rename to modules/nf-core/modules/samtools/index/functions.nf diff --git a/modules/nf-core/software/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf similarity index 100% rename from modules/nf-core/software/samtools/index/main.nf rename to 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modules/nf-core/software/samtools/merge/meta.yml rename to modules/nf-core/modules/samtools/merge/meta.yml diff --git a/modules/nf-core/software/samtools/sort/functions.nf b/modules/nf-core/modules/samtools/sort/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/sort/functions.nf rename to modules/nf-core/modules/samtools/sort/functions.nf diff --git a/modules/nf-core/software/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf similarity index 100% rename from modules/nf-core/software/samtools/sort/main.nf rename to modules/nf-core/modules/samtools/sort/main.nf diff --git a/modules/nf-core/software/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml similarity index 100% rename from modules/nf-core/software/samtools/sort/meta.yml rename to modules/nf-core/modules/samtools/sort/meta.yml diff --git a/modules/nf-core/software/samtools/stats/functions.nf b/modules/nf-core/modules/samtools/stats/functions.nf similarity index 100% rename from modules/nf-core/software/samtools/stats/functions.nf rename to modules/nf-core/modules/samtools/stats/functions.nf diff --git a/modules/nf-core/software/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf similarity index 100% rename from modules/nf-core/software/samtools/stats/main.nf rename to modules/nf-core/modules/samtools/stats/main.nf diff --git a/modules/nf-core/software/samtools/stats/meta.yml b/modules/nf-core/modules/samtools/stats/meta.yml similarity index 100% rename from modules/nf-core/software/samtools/stats/meta.yml rename to modules/nf-core/modules/samtools/stats/meta.yml diff --git a/modules/nf-core/software/star/align/functions.nf b/modules/nf-core/modules/star/align/functions.nf similarity index 100% rename from modules/nf-core/software/star/align/functions.nf rename to modules/nf-core/modules/star/align/functions.nf diff --git a/modules/nf-core/software/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf similarity index 100% rename from modules/nf-core/software/star/align/main.nf rename to modules/nf-core/modules/star/align/main.nf diff --git a/modules/nf-core/software/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml similarity index 100% rename from modules/nf-core/software/star/align/meta.yml rename to modules/nf-core/modules/star/align/meta.yml diff --git a/modules/nf-core/software/star/genomegenerate/functions.nf b/modules/nf-core/modules/star/genomegenerate/functions.nf similarity index 100% rename from modules/nf-core/software/star/genomegenerate/functions.nf rename to modules/nf-core/modules/star/genomegenerate/functions.nf diff --git a/modules/nf-core/software/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf similarity index 100% rename from modules/nf-core/software/star/genomegenerate/main.nf rename to modules/nf-core/modules/star/genomegenerate/main.nf diff --git a/modules/nf-core/software/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml similarity index 100% rename from modules/nf-core/software/star/genomegenerate/meta.yml rename to modules/nf-core/modules/star/genomegenerate/meta.yml diff --git a/modules/nf-core/software/untar/functions.nf b/modules/nf-core/modules/untar/functions.nf similarity index 100% rename from modules/nf-core/software/untar/functions.nf rename to modules/nf-core/modules/untar/functions.nf diff --git a/modules/nf-core/software/untar/main.nf b/modules/nf-core/modules/untar/main.nf similarity index 100% rename from modules/nf-core/software/untar/main.nf rename to modules/nf-core/modules/untar/main.nf diff --git a/modules/nf-core/software/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml similarity index 100% rename from modules/nf-core/software/untar/meta.yml rename to modules/nf-core/modules/untar/meta.yml From cfba18ebad6257d1a8bc6540d3e1ae95fbbc5356 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 16:04:30 +0200 Subject: [PATCH 023/235] fix: nf-core lint --- .github/CONTRIBUTING.md | 6 +++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/linting.yml | 2 +- bin/scrape_software_versions.py | 2 +- lib/NfcoreTemplate.groovy | 22 +++++++++++++--------- 5 files changed, 19 insertions(+), 15 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 34ca9f23..7b8f6f63 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/rnavar, the standard workflow is as * If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/rnavar repository](https://github.com/nf-core/rnavar) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) -4. Use `nf-core schema build .` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -69,7 +69,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build .`). +5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`). 6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. @@ -83,7 +83,7 @@ If you wish to contribute a new step, please use the following coding standards: Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. -Once there, use `nf-core schema build .` to add to `nextflow_schema.json`. +Once there, use `nf-core schema build` to add to `nextflow_schema.json`. ### Default processes resource requirements diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index a272d77d..8f2847a2 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnav - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnavar/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rnavar _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core lint .`). +- [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index fbde5cf8..3b448773 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -53,7 +53,7 @@ jobs: - uses: actions/setup-node@v1 with: - node-version: "10" + node-version: '10' - name: Install editorconfig-checker run: npm install -g editorconfig-checker diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py index 19d58215..77096990 100755 --- a/bin/scrape_software_versions.py +++ b/bin/scrape_software_versions.py @@ -30,7 +30,7 @@ print("
{}
{}
".format(k, v)) print(" ") -# Write out regexes as csv file: +# Write out as tsv file: with open("software_versions.tsv", "w") as f: for k, v in sorted(results.items()): f.write("{}\t{}\n".format(k, v)) diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index b6e689ec..44551e0a 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -24,17 +24,21 @@ class NfcoreTemplate { public static void hostName(workflow, params, log) { Map colors = logColours(params.monochrome_logs) if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.info "=${colors.yellow}====================================================${colors.reset}=\n" + - "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + - " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + - "=${colors.yellow}====================================================${colors.reset}=" + try { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } } } + } catch (Exception e) { + log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}." } } } From e06b7aad2b59b7d5162fb5f9aafbd87f4aa924e0 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 16:11:46 +0200 Subject: [PATCH 024/235] fix: update modules --- modules.json | 86 ++++++++++++++++++- .../nf-core/modules/cat/fastq/functions.nf | 68 +++++++++++++++ modules/nf-core/modules/cat/fastq/main.nf | 47 ++++++++++ modules/nf-core/modules/cat/fastq/meta.yml | 33 +++++++ .../nf-core/modules/gatk4/applybqsr/main.nf | 20 ++--- .../modules/gatk4/baserecalibrator/main.nf | 14 +-- .../gatk4/bedtointervallist/functions.nf | 32 ++++--- .../modules/gatk4/bedtointervallist/main.nf | 22 +++-- .../gatk4/createsequencedictionary/main.nf | 8 +- .../modules/gatk4/haplotypecaller/main.nf | 50 +---------- .../modules/gatk4/intervallisttools/main.nf | 24 +++--- .../nf-core/modules/gatk4/mergevcfs/main.nf | 22 ++--- .../modules/gatk4/splitncigarreads/main.nf | 22 ++--- .../modules/gatk4/variantfiltration/main.nf | 21 +++-- modules/nf-core/modules/hisat2/build/main.nf | 6 +- .../modules/hisat2/extractsplicesites/main.nf | 6 +- modules/nf-core/modules/multiqc/main.nf | 6 +- .../modules/picard/markduplicates/main.nf | 6 +- .../modules/rsem/preparereference/meta.yml | 37 ++++++++ modules/nf-core/modules/salmon/index/main.nf | 6 +- .../nf-core/modules/samtools/faidx/main.nf | 6 +- .../nf-core/modules/samtools/flagstat/main.nf | 6 +- .../nf-core/modules/samtools/idxstats/main.nf | 6 +- .../nf-core/modules/samtools/index/main.nf | 6 +- .../nf-core/modules/samtools/merge/main.nf | 14 +-- modules/nf-core/modules/samtools/sort/main.nf | 6 +- .../nf-core/modules/samtools/stats/main.nf | 6 +- modules/nf-core/modules/star/align/main.nf | 5 +- modules/nf-core/modules/star/align/meta.yml | 5 ++ .../modules/star/genomegenerate/main.nf | 6 +- 30 files changed, 408 insertions(+), 194 deletions(-) create mode 100644 modules/nf-core/modules/cat/fastq/functions.nf create mode 100644 modules/nf-core/modules/cat/fastq/main.nf create mode 100644 modules/nf-core/modules/cat/fastq/meta.yml create mode 100644 modules/nf-core/modules/rsem/preparereference/meta.yml diff --git a/modules.json b/modules.json index 0a3bd48e..1ae80d05 100644 --- a/modules.json +++ b/modules.json @@ -3,12 +3,96 @@ "homePage": "https://github.com/nf-core/rnavar", "repos": { "nf-core/modules": { + "cat/fastq": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, "fastqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, + "gatk4/applybqsr": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gatk4/baserecalibrator": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gatk4/bedtointervallist": { + "git_sha": "d624336cc1d219ff96d3cf00ab5933d2276bbdac" + }, + "gatk4/createsequencedictionary": { + "git_sha": "c8877835c288eb32b2ad1810d38a78e83e791f3d" + }, + "gatk4/haplotypecaller": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gatk4/intervallisttools": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gatk4/mergevcfs": { + "git_sha": "e7be649029b97d12ddf33baec8e85e141f3ece1c" + }, + "gatk4/splitncigarreads": { + "git_sha": "b3c466773207002060efbeff384b6f03ec0d7d9f" + }, + "gatk4/variantfiltration": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gffread": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "gunzip": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "hisat2/build": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "hisat2/extractsplicesites": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, "multiqc": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "picard/markduplicates": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "qualimap/bamqc": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "rsem/preparereference": { + "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" + }, + "salmon/index": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/faidx": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/flagstat": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/idxstats": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/index": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/merge": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/sort": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "samtools/stats": { + "git_sha": "c5235a983d454787fa0c3247b02086969217163b" + }, + "star/align": { + "git_sha": "df909015b6651dadb52626cf471f15a6d33985a2" + }, + "star/genomegenerate": { + "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc" + }, + "untar": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/cat/fastq/functions.nf b/modules/nf-core/modules/cat/fastq/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf new file mode 100644 index 00000000..55ccca90 --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process CAT_FASTQ { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.merged.fastq.gz"), emit: reads + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def readList = reads.collect{ it.toString() } + if (meta.single_end) { + if (readList.size > 1) { + """ + cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + """ + } + } else { + if (readList.size > 2) { + def read1 = [] + def read2 = [] + readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } + """ + cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + """ + } + } +} diff --git a/modules/nf-core/modules/cat/fastq/meta.yml b/modules/nf-core/modules/cat/fastq/meta.yml new file mode 100644 index 00000000..e7b8eebe --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/meta.yml @@ -0,0 +1,33 @@ +name: cat_fastq +description: Concatenates fastq files +keywords: + - fastq + - concatenate +tools: + - cat: + description: | + The cat utility reads files sequentially, writing them to the standard output. + documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FastQ files to be concatenated. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: Merged fastq file + pattern: "*.{merged.fastq.gz}" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index 941c2c7d..19b8c3d6 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -19,11 +19,12 @@ process GATK4_APPLYBQSR { } input: - tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervalsBed) - path fasta - path fastaidx - path dict - + tuple val(meta), path(bam), path(bai), path(bqsr_table) + path fasta + path fastaidx + path dict + path intervals + output: tuple val(meta), path("*.bam"), emit: bam path "*.version.txt" , emit: version @@ -31,17 +32,16 @@ process GATK4_APPLYBQSR { script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" - + def interval = intervals ? "-L ${intervals}" : "" """ gatk ApplyBQSR \\ -R $fasta \\ -I $bam \\ --bqsr-recal-file $bqsr_table \\ - $intervalsCommand \\ - -O ${prefix}.recalibrated.bam \\ + $interval \\ + -O ${prefix}.bam \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index 4ba1e78e..4e2730f2 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -24,33 +24,27 @@ process GATK4_BASERECALIBRATOR { path fastaidx path dict path intervalsBed - path dbsnp - path dbsnp_index path knownSites - path knownSites_index + path knownSites_tbi - //output: - //tuple val(meta), path("*.table"), emit: table output: - tuple val(meta), path("*.table") , emit: table - path "*.version.txt" , emit: version + tuple val(meta), path("*.table"), emit: table + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" - def dbsnpOptions = dbsnp ? "--known-sites ${dbsnp}" : "" def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') """ gatk BaseRecalibrator \ -R $fasta \ -I $bam \ - $dbsnpOptions \ $sitesCommand \ $intervalsCommand \ $options.args \ -O ${prefix}.table - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf index 9d0137e3..da9da093 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf @@ -1,19 +1,17 @@ -/* - * ----------------------------------------------------- - * Utility functions used in nf-core DSL2 module files - * ----------------------------------------------------- - */ +// +// Utility functions used in nf-core DSL2 module files +// -/* - * Extract name of software tool from process name using $task.process - */ +// +// Extract name of software tool from process name using $task.process +// def getSoftwareName(task_process) { return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() } -/* - * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules - */ +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// def initOptions(Map args) { def Map options = [:] options.args = args.args ?: '' @@ -26,18 +24,18 @@ def initOptions(Map args) { return options } -/* - * Tidy up and join elements of a list to return a path string - */ +// +// Tidy up and join elements of a list to return a path string +// def getPathFromList(path_list) { def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes return paths.join('/') } -/* - * Function to save/publish module results - */ +// +// Function to save/publish module results +// def saveFiles(Map args) { if (!args.filename.endsWith('.version.txt')) { def ioptions = initOptions(args.options) diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index 0f8021e1..af385f8f 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - // Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' @@ -7,30 +5,30 @@ params.options = [:] options = initOptions(params.options) process GATK4_BEDTOINTERVALLIST { - tag "$bed" + tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" } else { - container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + container "quay.io/biocontainers/gatk4:4.2.0.0--0" } input: - path bed + tuple val(meta), path(bed) path sequence_dict output: - path '*.interval_list' , emit: interval_list - path '*.version.txt' , emit: version + tuple val(meta), path('*.interval_list'), emit: interval_list + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${bed}.${options.suffix}" : "${bed}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ gatk BedToIntervalList \\ -I $bed \\ @@ -38,6 +36,6 @@ process GATK4_BEDTOINTERVALLIST { -O ${prefix}.interval_list \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index 6997fb5f..0276e8b2 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -11,11 +11,11 @@ process GATK4_CREATESEQUENCEDICTIONARY { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::gatk4=4.1.9.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0" + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" } else { - container "quay.io/biocontainers/gatk4:4.1.9.0--py39_0" + container "quay.io/biocontainers/gatk4:4.2.0.0--0" } input: @@ -40,6 +40,6 @@ process GATK4_CREATESEQUENCEDICTIONARY { --URI $fasta \\ $options.args - echo \$(gatk CreateSequenceDictionary --version 2>&1) | sed 's/^.*(GATK) v//; s/ HTSJDK.*\$//' > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf index 80906b79..c451de7f 100644 --- a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf +++ b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf @@ -19,53 +19,10 @@ process GATK4_HAPLOTYPECALLER { } input: - tuple val(meta), path(bam), path(bai), path(interval) - path dbsnp - path dbsnp_tbi - path dict - path fasta - path fai - val no_intervals - - output: - tuple val(meta), path("*.vcf"), emit: vcf - tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" - def avail_mem = 3 - if (!task.memory) { - log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - def intervalsOptions = no_intervals ? "" : "-L ${interval}" - def dbsnpOptions = dbsnp ? "--D ${dbsnp}" : "" - """ - gatk \\ - --java-options "-Xmx${avail_mem}g" \\ - HaplotypeCaller \\ - -R $fasta \\ - -I $bam \\ - ${intervalsOptions} \\ - ${dbsnpOptions} \\ - -O ${prefix}.vcf \\ - $options.args - - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt - """ -} - -/* - - input: - tuple val(meta), path(bam) + tuple val(meta), path(bam), path(bai) path fasta path fai path dict - path intervalsBed output: tuple val(meta), path("*.vcf.gz"), emit: vcf @@ -75,7 +32,6 @@ process GATK4_HAPLOTYPECALLER { script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" def avail_mem = 3 if (!task.memory) { log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -91,8 +47,6 @@ process GATK4_HAPLOTYPECALLER { -O ${prefix}.vcf.gz \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } - -*/ diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index 4c6eb99d..b2486eac 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -5,11 +5,11 @@ params.options = [:] options = initOptions(params.options) process GATK4_INTERVALLISTTOOLS { - tag "$interval_list" + tag "$meta.id" label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['']) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -19,35 +19,35 @@ process GATK4_INTERVALLISTTOOLS { } input: - path interval_list + tuple val(meta), path(interval_list) output: - path "*_split/*/*.interval_list" , emit: interval_list - path "*.version.txt" , emit: version + tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${interval_list}${options.suffix}" : "${interval_list}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ mkdir ${prefix}_split gatk \\ - IntervalListTools \\ - -I ${interval_list} \\ - -O ${prefix}_split \\ - $options.args + IntervalListTools \\ + -I ${interval_list} \\ + -O ${prefix}_split \\ + $options.args python3 < ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf index f64a298d..5a80c9ff 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -11,26 +11,26 @@ process GATK4_MERGEVCFS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" } else { - container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + container "quay.io/biocontainers/gatk4:4.2.0.0--0" } input: tuple val(meta), path(vcfs) - path(ref_dict) - val use_ref_dict + path ref_dict + val use_ref_dict output: - tuple val(meta), path('*.vcf.gz') , emit: vcf - tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi') , emit: vcf_index - path '*.version.txt' , emit: version + tuple val(meta), path('*.vcf.gz'), emit: vcf + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + // Make list of VCFs to merge def input = "" for (vcf in vcfs) { @@ -40,10 +40,10 @@ process GATK4_MERGEVCFS { """ gatk MergeVcfs \\ $input \\ - O=${prefix}.merged.vcf.gz \\ + O=${prefix}.vcf.gz \\ $ref \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf index 8be9efde..a8724e2e 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf +++ b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf @@ -1,5 +1,3 @@ -nextflow.enable.dsl=2 - // Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' @@ -13,33 +11,31 @@ process GATK4_SPLITNCIGARREADS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::gatk4=4.1.9.0' : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/gatk4:4.1.9.0--py39_0' + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" } else { - container 'quay.io/biocontainers/gatk4:4.1.9.0--py39_0' + container "quay.io/biocontainers/gatk4:4.2.0.0--0" } input: tuple val(meta), path(bam) - path fasta - path fai - path dict + tuple path(fasta), path(fai), path(dict) output: - tuple val(meta), path('*.split_cigar.bam'), emit: bam - path '*.version.txt' , emit: version + tuple val(meta), path('*.bam'), emit: bam + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ gatk SplitNCigarReads \\ -R $fasta \\ -I $bam \\ - -O ${prefix}.split_cigar.bam \\ + -O ${prefix}.bam \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index e8bf047c..82593d18 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -2,7 +2,7 @@ include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] -def options = initOptions(params.options) +options = initOptions(params.options) process GATK4_VARIANTFILTRATION { tag "$meta.id" @@ -19,27 +19,26 @@ process GATK4_VARIANTFILTRATION { } input: - tuple val(meta), path(vcf), path(tbi) - path fasta - path fai - path dict + tuple val(meta), path(vcf) + path fasta + path fai + path dict output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi') , emit: vcf_index - path "*.version.txt" , emit: version + tuple val(meta), path("*.vcf"), emit: vcf + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ gatk VariantFiltration \\ -R $fasta \\ -V $vcf \\ - -O ${prefix}.vcf.gz \\ + -O ${prefix}.vcf \\ $options.args - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/hisat2/build/main.nf b/modules/nf-core/modules/hisat2/build/main.nf index ce8bfb26..3e74b1d4 100644 --- a/modules/nf-core/modules/hisat2/build/main.nf +++ b/modules/nf-core/modules/hisat2/build/main.nf @@ -14,11 +14,11 @@ process HISAT2_BUILD { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::hisat2=2.2.0" : null) + conda (params.enable_conda ? 'bioconda::hisat2=2.2.1' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/hisat2:2.2.0--py37hfa133b6_4" + container "https://depot.galaxyproject.org/singularity/hisat2:2.2.1--h1b792b2_3" } else { - container "quay.io/biocontainers/hisat2:2.2.0--py37hfa133b6_4" + container "quay.io/biocontainers/hisat2:2.2.1--h1b792b2_3" } input: diff --git a/modules/nf-core/modules/hisat2/extractsplicesites/main.nf b/modules/nf-core/modules/hisat2/extractsplicesites/main.nf index 57f4dedb..d97fdb89 100644 --- a/modules/nf-core/modules/hisat2/extractsplicesites/main.nf +++ b/modules/nf-core/modules/hisat2/extractsplicesites/main.nf @@ -13,11 +13,11 @@ process HISAT2_EXTRACTSPLICESITES { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::hisat2=2.2.0" : null) + conda (params.enable_conda ? 'bioconda::hisat2=2.2.1' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/hisat2:2.2.0--py37hfa133b6_4" + container "https://depot.galaxyproject.org/singularity/hisat2:2.2.1--h1b792b2_3" } else { - container "quay.io/biocontainers/hisat2:2.2.0--py37hfa133b6_4" + container "quay.io/biocontainers/hisat2:2.2.1--h1b792b2_3" } input: diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf index da780800..8b6d6f0c 100644 --- a/modules/nf-core/modules/multiqc/main.nf +++ b/modules/nf-core/modules/multiqc/main.nf @@ -10,11 +10,11 @@ process MULTIQC { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null) + conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0" + container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" } else { - container "quay.io/biocontainers/multiqc:1.10.1--py_0" + container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" } input: diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index d7647414..d20014bf 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -11,11 +11,11 @@ process PICARD_MARKDUPLICATES { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::picard=2.23.9" : null) + conda (params.enable_conda ? 'bioconda::picard=2.25.7' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/picard:2.23.9--0" + container "https://depot.galaxyproject.org/singularity/picard:2.25.7--hdfd78af_0" } else { - container "quay.io/biocontainers/picard:2.23.9--0" + container "quay.io/biocontainers/picard:2.25.7--hdfd78af_0" } input: diff --git a/modules/nf-core/modules/rsem/preparereference/meta.yml b/modules/nf-core/modules/rsem/preparereference/meta.yml new file mode 100644 index 00000000..d7c02154 --- /dev/null +++ b/modules/nf-core/modules/rsem/preparereference/meta.yml @@ -0,0 +1,37 @@ +name: rsem_preparereference +description: Prepare a reference genome for RSEM +keywords: + - rsem + - reference +tools: + - rseqc: + description: | + RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome + homepage: https://github.com/deweylab/RSEM + documentation: https://github.com/deweylab/RSEM + doi: https://doi.org/10.1186/1471-2105-12-323 +input: + - fasta: + type: file + description: The Fasta file of the reference genome + pattern: "*.{fasta,fa}" + - gtf: + type: file + description: The GTF file of the reference genome + pattern: "*.gtf" +output: + - rsem: + type: directory + description: RSEM index directory + pattern: "rsem" + - transcript_fasta: + type: file + description: Fasta file of transcripts + pattern: "rsem/*transcripts.fa" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@kevinmenden" diff --git a/modules/nf-core/modules/salmon/index/main.nf b/modules/nf-core/modules/salmon/index/main.nf index 17d5bc06..e72ff121 100644 --- a/modules/nf-core/modules/salmon/index/main.nf +++ b/modules/nf-core/modules/salmon/index/main.nf @@ -11,11 +11,11 @@ process SALMON_INDEX { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::salmon=1.4.0" : null) + conda (params.enable_conda ? 'bioconda::salmon=1.5.2' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/salmon:1.4.0--hf69c8f4_0" + container "https://depot.galaxyproject.org/singularity/salmon:1.5.2--h84f40af_0" } else { - container "quay.io/biocontainers/salmon:1.4.0--hf69c8f4_0" + container "quay.io/biocontainers/salmon:1.5.2--h84f40af_0" } input: diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index 6c023f1c..a89ff2bb 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_FAIDX { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::samtools=1.12" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.12--hd5e65b6_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.12--hd5e65b6_0" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index 7260f502..d4852c66 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_FLAGSTAT { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index eb74a25e..14a07cfb 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_IDXSTATS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index 2f323c81..e1966fb3 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_INDEX { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index 81b2828c..0182b9fd 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -11,25 +11,25 @@ process SAMTOOLS_MERGE { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.12" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.12--hd5e65b6_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.12--hd5e65b6_0" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: tuple val(meta), path(bams) output: - tuple val(meta), path("*merged.bam"), emit: merged_bam - path "*.version.txt" , emit: version + tuple val(meta), path("${prefix}.bam"), emit: bam + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ - samtools merge ${prefix}_merged.bam $bams + samtools merge ${prefix}.bam $bams echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index ee09c656..0a6b7048 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_SORT { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index c307f9e8..8c72d725 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_STATS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" } input: diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf index 1760ff16..c06daf24 100644 --- a/modules/nf-core/modules/star/align/main.nf +++ b/modules/nf-core/modules/star/align/main.nf @@ -12,7 +12,7 @@ process STAR_ALIGN { saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? 'bioconda::star=2.6.1d' : null) + conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' } else { @@ -36,12 +36,13 @@ process STAR_ALIGN { tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq tuple val(meta), path('*.tab') , optional:true, emit: tab + tuple val(meta), path('*.out.junction') , optional:true, emit: junction script: def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' 'PL:illumina'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix' 'PL:illumina'" + def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' """ diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml index 01bc2ecf..a589d145 100644 --- a/modules/nf-core/modules/star/align/meta.yml +++ b/modules/nf-core/modules/star/align/meta.yml @@ -69,7 +69,12 @@ output: type: file description: STAR output tab file(s) (optional) pattern: "*.tab" + - junction: + type: file + description: STAR chimeric junction output file (optional) + pattern: "*.out.junction" authors: - "@kevinmenden" - "@drpatelh" + - "@praveenraj2018" diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf index b440b415..9335b9b5 100644 --- a/modules/nf-core/modules/star/genomegenerate/main.nf +++ b/modules/nf-core/modules/star/genomegenerate/main.nf @@ -12,11 +12,11 @@ process STAR_GENOMEGENERATE { saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0" : null) + conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0" + container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" } else { - container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0" + container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" } input: From 7bb0768db26feaeb2234e9933ef481418564f88f Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 16:17:13 +0200 Subject: [PATCH 025/235] fix: EClint --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index 1ae80d05..7920de5e 100644 --- a/modules.json +++ b/modules.json @@ -95,4 +95,4 @@ } } } -} \ No newline at end of file +} From 1871a5cf3dedfd67f3a6114be42f0947eb69239f Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 16:18:13 +0200 Subject: [PATCH 026/235] fix: remove module --- modules/nf-core/modules/fastq/functions.nf | 68 ---------------------- modules/nf-core/modules/fastq/main.nf | 47 --------------- modules/nf-core/modules/fastq/meta.yml | 33 ----------- 3 files changed, 148 deletions(-) delete mode 100644 modules/nf-core/modules/fastq/functions.nf delete mode 100644 modules/nf-core/modules/fastq/main.nf delete mode 100644 modules/nf-core/modules/fastq/meta.yml diff --git a/modules/nf-core/modules/fastq/functions.nf b/modules/nf-core/modules/fastq/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/fastq/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/fastq/main.nf b/modules/nf-core/modules/fastq/main.nf deleted file mode 100644 index 55ccca90..00000000 --- a/modules/nf-core/modules/fastq/main.nf +++ /dev/null @@ -1,47 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process CAT_FASTQ { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.merged.fastq.gz"), emit: reads - - script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def readList = reads.collect{ it.toString() } - if (meta.single_end) { - if (readList.size > 1) { - """ - cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz - """ - } - } else { - if (readList.size > 2) { - def read1 = [] - def read2 = [] - readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } - """ - cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz - cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz - """ - } - } -} diff --git a/modules/nf-core/modules/fastq/meta.yml b/modules/nf-core/modules/fastq/meta.yml deleted file mode 100644 index e7b8eebe..00000000 --- a/modules/nf-core/modules/fastq/meta.yml +++ /dev/null @@ -1,33 +0,0 @@ -name: cat_fastq -description: Concatenates fastq files -keywords: - - fastq - - concatenate -tools: - - cat: - description: | - The cat utility reads files sequentially, writing them to the standard output. - documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: list - description: | - List of input FastQ files to be concatenated. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: Merged fastq file - pattern: "*.{merged.fastq.gz}" -authors: - - "@joseespinosa" - - "@drpatelh" From 15d377f865279abcd075c909a3345402d924a958 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 17:25:24 +0200 Subject: [PATCH 027/235] fix: right paths to modules --- conf/modules.config | 7 ++- .../gatk4/applybqsr/functions.nf | 0 .../modules => local}/gatk4/applybqsr/main.nf | 3 +- .../gatk4/applybqsr/meta.yml | 0 .../gatk4/haplotypecaller/functions.nf | 0 .../gatk4/haplotypecaller/main.nf | 30 +++++++---- .../gatk4/haplotypecaller/meta.yml | 0 nextflow.config | 5 +- subworkflows/local/prepare_genome.nf | 29 +++++----- subworkflows/nf-core/align_star.nf | 4 +- subworkflows/nf-core/bam_sort_samtools.nf | 6 +-- subworkflows/nf-core/bam_stats_samtools.nf | 6 +-- .../nf-core/mark_duplicates_picard.nf | 6 +-- subworkflows/nf-core/recalibrate.nf | 12 ++--- subworkflows/nf-core/splitn_cigar_reads.nf | 4 +- workflows/rnavar.nf | 53 +++++++++---------- 16 files changed, 87 insertions(+), 78 deletions(-) rename modules/{nf-core/modules => local}/gatk4/applybqsr/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/applybqsr/main.nf (94%) rename modules/{nf-core/modules => local}/gatk4/applybqsr/meta.yml (100%) rename modules/{nf-core/modules => local}/gatk4/haplotypecaller/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/haplotypecaller/main.nf (58%) rename modules/{nf-core/modules => local}/gatk4/haplotypecaller/meta.yml (100%) diff --git a/conf/modules.config b/conf/modules.config index c008f297..671b8bd6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -125,5 +125,10 @@ params { publish_dir = 'variant_calling' publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] } - } + 'fastqc' { + args = "--quiet" + } + 'multiqc' { + args = "" + } } } diff --git a/modules/nf-core/modules/gatk4/applybqsr/functions.nf b/modules/local/gatk4/applybqsr/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/applybqsr/functions.nf rename to modules/local/gatk4/applybqsr/functions.nf diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/local/gatk4/applybqsr/main.nf similarity index 94% rename from modules/nf-core/modules/gatk4/applybqsr/main.nf rename to modules/local/gatk4/applybqsr/main.nf index 19b8c3d6..8e10a5f2 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/local/gatk4/applybqsr/main.nf @@ -19,11 +19,10 @@ process GATK4_APPLYBQSR { } input: - tuple val(meta), path(bam), path(bai), path(bqsr_table) + tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervals) path fasta path fastaidx path dict - path intervals output: tuple val(meta), path("*.bam"), emit: bam diff --git a/modules/nf-core/modules/gatk4/applybqsr/meta.yml b/modules/local/gatk4/applybqsr/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/applybqsr/meta.yml rename to modules/local/gatk4/applybqsr/meta.yml diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/functions.nf b/modules/local/gatk4/haplotypecaller/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/haplotypecaller/functions.nf rename to modules/local/gatk4/haplotypecaller/functions.nf diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf similarity index 58% rename from modules/nf-core/modules/gatk4/haplotypecaller/main.nf rename to modules/local/gatk4/haplotypecaller/main.nf index c451de7f..d88bd095 100644 --- a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf +++ b/modules/local/gatk4/haplotypecaller/main.nf @@ -6,7 +6,7 @@ options = initOptions(params.options) process GATK4_HAPLOTYPECALLER { tag "$meta.id" - label 'process_medium' + label 'process_low' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } @@ -19,34 +19,42 @@ process GATK4_HAPLOTYPECALLER { } input: - tuple val(meta), path(bam), path(bai) + tuple val(meta), path(cram), path(crai), path(interval) + path dbsnp + path dbsnp_tbi + path dict path fasta path fai - path dict + val no_intervals output: - tuple val(meta), path("*.vcf.gz"), emit: vcf - tuple val(meta), path("*.tbi") , emit: tbi - path "*.version.txt" , emit: version + tuple val(meta), path("*.vcf") , emit: vcf + tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf + path "*.version.txt" , emit: version script: def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" def avail_mem = 3 if (!task.memory) { log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } + def intervalsOptions = no_intervals ? "" : "-L ${interval}" + def dbsnpOptions = params.dbsnp ? "-D ${dbsnp}" : "" + //TODO allow ploidy argument here since we allow it for the cnv callers? or is this covered with options? Might unintuitive to use """ gatk \\ --java-options "-Xmx${avail_mem}g" \\ HaplotypeCaller \\ -R $fasta \\ - -I $bam \\ - -O ${prefix}.vcf.gz \\ + -I $cram \\ + ${dbsnpOptions} \\ + ${intervalsOptions} \\ + -O ${prefix}.vcf \\ + --tmp-dir . \ $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/meta.yml b/modules/local/gatk4/haplotypecaller/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/haplotypecaller/meta.yml rename to modules/local/gatk4/haplotypecaller/meta.yml diff --git a/nextflow.config b/nextflow.config index b0b1ed7d..58ee6bc2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -20,6 +20,7 @@ params { // Alignment aligner = 'star' + star_ignore_sjdbgtf = false seq_center = null save_merged_fastq = false save_unaligned = false @@ -41,8 +42,8 @@ params { //GATK variant filter parameters window = 35 cluster = 3 - fs_filter = 30.0 - qd_filter = 2.0 + fs_filter = 30 + qd_filter = 2 // QC skip_qc = false diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index ae280892..26870056 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -16,25 +16,24 @@ include { GUNZIP as GUNZIP_GFF GUNZIP as GUNZIP_GENE_BED GUNZIP as GUNZIP_TRANSCRIPT_FASTA - GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/software/gunzip/main' addParams( options: params.genome_options ) -include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.star_index_options ) -include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.index_options ) -include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.hisat2_index_options ) -include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/software/untar/main' addParams( options: params.index_options ) -include { GFFREAD } from '../../modules/nf-core/software/gffread/main' addParams( options: params.gffread_options ) -include { STAR_GENOMEGENERATE } from '../../modules/nf-core/software/star/genomegenerate/main' addParams( options: params.star_index_options ) -include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/software/hisat2/extractsplicesites/main' addParams( options: params.hisat2_index_options ) -include { HISAT2_BUILD } from '../../modules/nf-core/software/hisat2/build/main' addParams( options: params.hisat2_index_options ) -include { SALMON_INDEX } from '../../modules/nf-core/software/salmon/index/main' addParams( options: params.salmon_index_options ) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/software/rsem/preparereference/main' addParams( options: params.rsem_index_options ) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/software/rsem/preparereference/main' addParams( options: params.genome_options ) - + GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams( options: params.genome_options ) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.star_index_options ) +include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.index_options ) +include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.hisat2_index_options ) +include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.index_options ) +include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams( options: params.gffread_options ) +include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams( options: params.star_index_options ) +include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/modules/hisat2/extractsplicesites/main' addParams( options: params.hisat2_index_options ) +include { HISAT2_BUILD } from '../../modules/nf-core/modules/hisat2/build/main' addParams( options: params.hisat2_index_options ) +include { SALMON_INDEX } from '../../modules/nf-core/modules/salmon/index/main' addParams( options: params.salmon_index_options ) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams( options: params.rsem_index_options ) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams( options: params.genome_options ) include { GTF2BED } from '../../modules/local/gtf2bed' addParams( options: params.genome_options ) include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams( options: params.genome_options ) include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams( options: params.genome_options ) include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams( options: params.genome_options ) -include { SAMTOOLS_FAIDX } from '../../modules/nf-core/software/samtools/faidx/main' addParams( options: params.genome_options ) -include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/software/gatk4/createsequencedictionary/main' addParams( options: params.genome_options ) +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams( options: params.genome_options ) +include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams( options: params.genome_options ) workflow PREPARE_GENOME { take: diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index 7d228e1a..40cc68b6 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -7,8 +7,8 @@ params.samtools_sort_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { STAR_ALIGN } from '../../modules/nf-core/software/star/align/main' addParams( options: params.align_options ) -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options ) +include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) workflow ALIGN_STAR { take: diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index 4ae896dc..ba4d39ef 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -6,9 +6,9 @@ params.sort_options = [:] params.index_options = [:] params.stats_options = [:] -include { SAMTOOLS_SORT } from '../../modules/nf-core/software/samtools/sort/main' addParams( options: params.sort_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) +include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' addParams( options: params.sort_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) workflow BAM_SORT_SAMTOOLS { take: diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index eca9b37a..ca05241c 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -4,9 +4,9 @@ params.options = [:] -include { SAMTOOLS_STATS } from '../../modules/nf-core/software/samtools/stats/main' addParams( options: params.options ) -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/software/samtools/idxstats/main' addParams( options: params.options ) -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/software/samtools/flagstat/main' addParams( options: params.options ) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams( options: params.options ) +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' addParams( options: params.options ) +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' addParams( options: params.options ) workflow BAM_STATS_SAMTOOLS { take: diff --git a/subworkflows/nf-core/mark_duplicates_picard.nf b/subworkflows/nf-core/mark_duplicates_picard.nf index 7e59ac68..2e8b676d 100644 --- a/subworkflows/nf-core/mark_duplicates_picard.nf +++ b/subworkflows/nf-core/mark_duplicates_picard.nf @@ -6,9 +6,9 @@ params.markduplicates_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/software/picard/markduplicates/main' addParams( options: params.markduplicates_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.samtools_index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) workflow MARK_DUPLICATES_PICARD { take: diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 943a550f..61f4459d 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -10,11 +10,11 @@ params.qualimap_bamqc_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/software/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) -include { QUALIMAP_BAMQC } from '../../modules/nf-core/software/qualimap/bamqc/main' addParams(options: params.qualimap_bamqc_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams(options: params.samtools_index_options) -include { SAMTOOLS_MERGE } from '../../modules/nf-core/software/samtools/merge/main' addParams(options: params.merge_bam_options) -include { SAMTOOLS_STATS } from '../../modules/nf-core/software/samtools/stats/main' addParams(options: params.samtools_stats_options) +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/local/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) +include { QUALIMAP_BAMQC } from '../../modules/nf-core/modules/qualimap/bamqc/main' addParams(options: params.qualimap_bamqc_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) workflow RECALIBRATE { take: @@ -50,7 +50,7 @@ workflow RECALIBRATE { }.groupTuple().set{bam_recalibrated_interval} SAMTOOLS_MERGE(bam_recalibrated_interval) - bam_recalibrated = SAMTOOLS_MERGE.out.merged_bam + bam_recalibrated = SAMTOOLS_MERGE.out.bam SAMTOOLS_INDEX(bam_recalibrated) bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf index 7e59ac68..317944ef 100644 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -6,8 +6,8 @@ params.markduplicates_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/software/picard/markduplicates/main' addParams( options: params.markduplicates_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/software/samtools/index/main' addParams( options: params.samtools_index_options ) +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) workflow MARK_DUPLICATES_PICARD { diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 20062907..506f7e7c 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -126,26 +126,25 @@ if (params.cluster) { gatk_variant_filter_options.args += Utils.joinModuleArg if (params.fs_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) } if (params.qd_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) } -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) -include { CAT_FASTQ } from '../modules/nf-core/software/cat/fastq/main' addParams( options: cat_fastq_options ) -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/software/gatk4/bedtointervallist/main' addParams( options: [:]) -include { SAMTOOLS_INDEX } from '../modules/nf-core/software/samtools/index/main' addParams( options: samtools_index_genome_options ) -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) -include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) -include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/software/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/software/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) -include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams( options: cat_fastq_options ) +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams( options: [:]) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( options: samtools_index_genome_options ) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) +include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) +include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/modules/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( applybqsr_options: modules['applybqsr'], merge_bam_options: modules['merge_bam_recalibrate'], qualimap_bamqc_options: modules['qualimap_bamqc_recalibrate'], samtools_index_options: modules['samtools_index_recalibrate'], samtools_stats_options: modules['samtools_stats_recalibrate'] ) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/software/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) -include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/software/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) -include { GATK4_MERGEVCFS } from '../modules/nf-core/software/gatk4/mergevcfs/main' addParams( options: modules['gatk_mergevcfs']) -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/software/gatk4/variantfiltration/main' addParams( options: gatk_variant_filter_options) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) +include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams( options: modules['gatk_mergevcfs']) +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams( options: gatk_variant_filter_options) // Info required for completion email and summary def multiqc_report = [] @@ -158,12 +157,11 @@ workflow RNAVAR { prepareToolIndices, biotype ) - ch_genome_gtf = Channel.empty() - ch_genome_gtf = PREPARE_GENOME.out.gene_bed + ch_genome_gtf = Channel.from([id:"BED"]).join(PREPARE_GENOME.out.gene_bed) + ch_software_versions = Channel.empty() ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) - INPUT_CHECK ( ch_input ) @@ -184,8 +182,8 @@ workflow RNAVAR { // // MODULE: Run FastQC // - FASTQC(ch_fastq) - ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + // FASTQC(ch_fastq) + // ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) // MODULE: Concatenate FastQ files from same sample if required // @@ -256,11 +254,11 @@ workflow RNAVAR { bam_splitncigar = Channel.empty() if(!params.skip_splitncigar){ + splitncigarreads_reference = PREPARE_GENOME.out.fasta.join(PREPARE_GENOME.out.fai).join(PREPARE_GENOME.out.dict) + GATK4_SPLITNCIGARREADS ( ch_genome_bam, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict + splitncigarreads_reference ) SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) @@ -274,16 +272,15 @@ workflow RNAVAR { ch_bqsr_table = Channel.empty() if(!params.skip_basecalibrator){ + GATK4_BASERECALIBRATOR( bam_splitncigar, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - params.known_indels, - params.known_indels_index + params.dbsnp_vcf.join(params.known_indels), + params.dbsnp_vcf_index.join(params.known_indels_index) ) ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table } @@ -360,7 +357,7 @@ workflow RNAVAR { use_ref_dict ) - haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf_index + haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf } if(!params.skip_variantfiltration){ @@ -404,7 +401,7 @@ workflow RNAVAR { ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) MULTIQC ( ch_multiqc_files.collect() From fb288751f920eda818120ef1c558e12eabbb13e9 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 28 Jul 2021 17:28:18 +0200 Subject: [PATCH 028/235] fix: nf-core lint --- modules.json | 6 ------ nextflow_schema.json | 3 +++ 2 files changed, 3 insertions(+), 6 deletions(-) diff --git a/modules.json b/modules.json index 7920de5e..89081642 100644 --- a/modules.json +++ b/modules.json @@ -9,9 +9,6 @@ "fastqc": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, - "gatk4/applybqsr": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, "gatk4/baserecalibrator": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, @@ -21,9 +18,6 @@ "gatk4/createsequencedictionary": { "git_sha": "c8877835c288eb32b2ad1810d38a78e83e791f3d" }, - "gatk4/haplotypecaller": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, "gatk4/intervallisttools": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, diff --git a/nextflow_schema.json b/nextflow_schema.json index f4a0adaa..b2c589a9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -406,6 +406,9 @@ }, "star_index": { "type": "string" + }, + "star_ignore_sjdbgtf": { + "type": "string" } } } From 1bbfb811333d45bc77a68dc12d5043cfb0df6497 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Thu, 29 Jul 2021 16:15:22 +0200 Subject: [PATCH 029/235] fix: rename some files --- CHANGELOG.md | 2 +- conf/modules.config | 2 +- modules/local/gatk4/haplotypecaller/main.nf | 6 +- .../gatk4/splitncigarreads/functions.nf | 0 .../gatk4/splitncigarreads/main.nf | 8 +- .../gatk4/splitncigarreads/meta.yml | 0 subworkflows/local/prepare_genome.nf | 53 ++-- ...duplicates_picard.nf => markduplicates.nf} | 32 +- workflows/rnavar.nf | 283 ++++++++---------- 9 files changed, 173 insertions(+), 213 deletions(-) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/main.nf (87%) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/meta.yml (100%) rename subworkflows/nf-core/{mark_duplicates_picard.nf => markduplicates.nf} (68%) diff --git a/CHANGELOG.md b/CHANGELOG.md index 1d65fac4..cb967546 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v3.1 - [date] +## v1.0dev - [date] Initial release of nf-core/rnavar, created with the [nf-core](https://nf-co.re/) template. diff --git a/conf/modules.config b/conf/modules.config index 671b8bd6..cd3e6761 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -58,7 +58,7 @@ params { } 'gatk_splitncigar_options' { args = '' - suffix = '.split_cigar.bam' + suffix = '.split_cigar' publish_files = ['bam': '', 'bai': ''] publish_dir = "${params.aligner}" } diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf index d88bd095..3a775e6c 100644 --- a/modules/local/gatk4/haplotypecaller/main.nf +++ b/modules/local/gatk4/haplotypecaller/main.nf @@ -19,7 +19,7 @@ process GATK4_HAPLOTYPECALLER { } input: - tuple val(meta), path(cram), path(crai), path(interval) + tuple val(meta), path(bam), path(bai), path(interval) path dbsnp path dbsnp_tbi path dict @@ -49,12 +49,12 @@ process GATK4_HAPLOTYPECALLER { --java-options "-Xmx${avail_mem}g" \\ HaplotypeCaller \\ -R $fasta \\ - -I $cram \\ + -I $bam \\ ${dbsnpOptions} \\ ${intervalsOptions} \\ -O ${prefix}.vcf \\ - --tmp-dir . \ $options.args + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt """ } diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/splitncigarreads/functions.nf rename to modules/local/gatk4/splitncigarreads/functions.nf diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/local/gatk4/splitncigarreads/main.nf similarity index 87% rename from modules/nf-core/modules/gatk4/splitncigarreads/main.nf rename to modules/local/gatk4/splitncigarreads/main.nf index a8724e2e..d1c328ea 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf +++ b/modules/local/gatk4/splitncigarreads/main.nf @@ -20,11 +20,13 @@ process GATK4_SPLITNCIGARREADS { input: tuple val(meta), path(bam) - tuple path(fasta), path(fai), path(dict) + path(fasta) + path(fai) + path(dict) output: - tuple val(meta), path('*.bam'), emit: bam - path '*.version.txt' , emit: version + tuple val(meta), path('*.bam'), path('*.bai'), emit: bam + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/splitncigarreads/meta.yml rename to modules/local/gatk4/splitncigarreads/meta.yml diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 26870056..9b32a48c 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -3,37 +3,36 @@ // params.genome_options = [:] -params.index_options = [:] params.gffread_options = [:] -params.star_index_options = [:] -params.rsem_index_options = [:] params.hisat2_index_options = [:] +params.index_options = [:] +params.rsem_index_options = [:] params.salmon_index_options = [:] +params.star_index_options = [:] -include { - GUNZIP as GUNZIP_FASTA - GUNZIP as GUNZIP_GTF - GUNZIP as GUNZIP_GFF - GUNZIP as GUNZIP_GENE_BED - GUNZIP as GUNZIP_TRANSCRIPT_FASTA - GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams( options: params.genome_options ) -include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.star_index_options ) -include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.index_options ) -include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.hisat2_index_options ) -include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/modules/untar/main' addParams( options: params.index_options ) -include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams( options: params.gffread_options ) -include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams( options: params.star_index_options ) -include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/modules/hisat2/extractsplicesites/main' addParams( options: params.hisat2_index_options ) -include { HISAT2_BUILD } from '../../modules/nf-core/modules/hisat2/build/main' addParams( options: params.hisat2_index_options ) -include { SALMON_INDEX } from '../../modules/nf-core/modules/salmon/index/main' addParams( options: params.salmon_index_options ) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams( options: params.rsem_index_options ) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams( options: params.genome_options ) -include { GTF2BED } from '../../modules/local/gtf2bed' addParams( options: params.genome_options ) -include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams( options: params.genome_options ) -include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams( options: params.genome_options ) -include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams( options: params.genome_options ) -include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams( options: params.genome_options ) -include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams( options: params.genome_options ) +include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams(options: params.genome_options) +include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) +include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) +include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams(options: params.gffread_options) +include { GTF2BED } from '../../modules/local/gtf2bed' addParams(options: params.genome_options) +include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_TRANSCRIPT_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { HISAT2_BUILD } from '../../modules/nf-core/modules/hisat2/build/main' addParams(options: params.hisat2_index_options) +include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/modules/hisat2/extractsplicesites/main' addParams(options: params.hisat2_index_options) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams(options: params.rsem_index_options) +include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams(options: params.genome_options) +include { SALMON_INDEX } from '../../modules/nf-core/modules/salmon/index/main' addParams(options: params.salmon_index_options) +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) +include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) +include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.hisat2_index_options) +include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.index_options) +include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.index_options) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_index_options) workflow PREPARE_GENOME { take: diff --git a/subworkflows/nf-core/mark_duplicates_picard.nf b/subworkflows/nf-core/markduplicates.nf similarity index 68% rename from subworkflows/nf-core/mark_duplicates_picard.nf rename to subworkflows/nf-core/markduplicates.nf index 2e8b676d..00a05d0d 100644 --- a/subworkflows/nf-core/mark_duplicates_picard.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -6,40 +6,32 @@ params.markduplicates_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams(options: params.samtools_stats_options) +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams(options: params.markduplicates_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) -workflow MARK_DUPLICATES_PICARD { +workflow MARKDUPLICATES { take: bam // channel: [ val(meta), [ bam ] ] main: - // - // Picard MarkDuplicates - // - PICARD_MARKDUPLICATES ( bam ) + PICARD_MARKDUPLICATES(bam) // // Index BAM file and run samtools stats, flagstat and idxstats // - SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) PICARD_MARKDUPLICATES.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map { - meta, bam, bai, csi -> - if (bai) { - [ meta, bam, bai ] - } else { - [ meta, bam, csi ] - } - } - .set { ch_bam_bai } - - BAM_STATS_SAMTOOLS ( ch_bam_bai ) + .map{meta, bam, bai, csi -> + if (bai) [meta, bam, bai] + else [meta, bam, csi]} + .set{ch_bam_bai} + + BAM_STATS_SAMTOOLS(ch_bam_bai) emit: bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 506f7e7c..fea00dec 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -19,10 +19,11 @@ def checkPathParamList = [ params.gene_bed, params.star_index] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } +for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} // Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } +if (params.input) ch_input = file(params.input) +else exit 1, 'Input samplesheet not specified!' /* ======================================================================================== @@ -45,12 +46,12 @@ def modules = params.modules.clone() // // MODULE: Local to the pipeline // -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams(options: [publish_files : ['tsv':'']]) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams(options: [:]) /* ======================================================================================== @@ -58,14 +59,13 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( opti ======================================================================================== */ -def multiqc_options = modules['multiqc'] -multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' +modules['multiqc'].args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams(options: modules['fastqc']) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams(options: modules['multiqc']) /* ======================================================================================== @@ -75,76 +75,78 @@ include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( op // Check alignment parameters def prepareToolIndices = [] -if (!params.skip_alignment) { prepareToolIndices << params.aligner } +if (!params.skip_alignment) { prepareToolIndices = params.aligner } def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] +if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false -def star_genomegenerate_options = modules['star_genomegenerate'] -if (!params.save_reference) { star_genomegenerate_options['publish_files'] = false } - -def samtools_sort_genome_options = modules['samtools_sort_genome'] -def samtools_index_genome_options = modules['samtools_index_genome'] - -samtools_index_genome_options.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' if (['star'].contains(params.aligner)) { if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { - samtools_sort_genome_options.publish_files.put('bam','') - samtools_index_genome_options.publish_files.put('bai','') - samtools_index_genome_options.publish_files.put('csi','') + modules['samtools_sort_genome'].publish_files.put('bam','') + modules['samtools_index_genome'].publish_files.put('bai','') + modules['samtools_index_genome'].publish_files.put('csi','') } } else { if (params.save_align_intermeds || params.skip_markduplicates) { - samtools_sort_genome_options.publish_files.put('bam','') - samtools_index_genome_options.publish_files.put('bai','') - samtools_index_genome_options.publish_files.put('csi','') + modules['samtools_sort_genome'].publish_files.put('bam','') + modules['samtools_index_genome'].publish_files.put('bai','') + modules['samtools_index_genome'].publish_files.put('csi','') } } -def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type - -def cat_fastq_options = modules['cat_fastq'] -if (!params.save_merged_fastq) { cat_fastq_options['publish_files'] = false } - -def star_align_options = modules['star_align'] -star_align_options.args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' -if (params.save_align_intermeds) { star_align_options.publish_files.put('bam','') } -if (params.save_unaligned) { star_align_options.publish_files.put('fastq.gz','unmapped') } - -def picard_markduplicates_samtools = modules['picard_markduplicates_samtools'] -picard_markduplicates_samtools.args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' - -def intervaltools_options = modules['gatk_intervallisttools'] -intervaltools_options.args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) - - -def gatk_variant_filter_options = modules['gatk_variantfilter'] -if (params.window) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--window $params.window"]) } -if (params.cluster) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--cluster $params.cluster"]) } -if (params.fs_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) } -if (params.qd_filter) { gatk_variant_filter_options.args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) } - -include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams( options: cat_fastq_options ) -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: star_genomegenerate_options) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams( options: [:]) -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams( options: samtools_index_genome_options ) -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: star_align_options, samtools_sort_options: samtools_sort_genome_options, samtools_index_options: samtools_index_genome_options, samtools_stats_options: samtools_index_genome_options ) -include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: picard_markduplicates_samtools, samtools_stats_options: picard_markduplicates_samtools ) -include { GATK4_SPLITNCIGARREADS } from '../modules/nf-core/modules/gatk4/splitncigarreads/main' addParams( options: publish_genome_options, splitncigar_options: modules['gatk_splitncigar_options'] ) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams( options: modules['gatk_baserecalibrator']) -include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( - applybqsr_options: modules['applybqsr'], - merge_bam_options: modules['merge_bam_recalibrate'], - qualimap_bamqc_options: modules['qualimap_bamqc_recalibrate'], - samtools_index_options: modules['samtools_index_recalibrate'], - samtools_stats_options: modules['samtools_stats_recalibrate'] +def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type + +if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false + +modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') +if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') + +modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) + +if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) +if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) +if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) +if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) + +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: [:]) +include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) +include { GATK4_SPLITNCIGARREADS } from '../modules/local/gatk4/splitncigarreads/main' addParams(options: modules['gatk_splitncigar_options']) +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) + +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( + genome_options: publish_genome_options, + index_options: publish_index_options, + star_index_options: modules['star_genomegenerate'] +) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( + align_options: modules['star_align'], + samtools_index_options: modules['samtools_index_genome'], + samtools_sort_options: modules['samtools_sort_genome'], + samtools_stats_options: modules['samtools_index_genome'] +) +include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( + markduplicates_options: modules['picard_markduplicates'], + samtools_index_options: modules['picard_markduplicates_samtools'], + samtools_stats_options: modules['picard_markduplicates_samtools'] +) +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( + applybqsr_options: modules['applybqsr'], + merge_bam_options: modules['merge_bam_recalibrate'], + qualimap_bamqc_options: modules['qualimap_bamqc_recalibrate'], + samtools_index_options: modules['samtools_index_recalibrate'], + samtools_stats_options: modules['samtools_stats_recalibrate'] ) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams( options: intervaltools_options ) -include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams( options: modules['gatk_haplotypecaller']) -include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams( options: modules['gatk_mergevcfs']) -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams( options: gatk_variant_filter_options) // Info required for completion email and summary def multiqc_report = [] @@ -153,31 +155,23 @@ workflow RNAVAR { ch_software_versions = Channel.empty() - PREPARE_GENOME ( - prepareToolIndices, - biotype - ) - ch_genome_gtf = Channel.from([id:"BED"]).join(PREPARE_GENOME.out.gene_bed) + PREPARE_GENOME(prepareToolIndices, biotype) + ch_genome_gtf = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) ch_software_versions = Channel.empty() ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) - INPUT_CHECK ( - ch_input - ) - .map { - meta, fastq -> + INPUT_CHECK(ch_input) + .map{ meta, fastq -> meta.id = meta.id.split('_')[0..-2].join('_') - [ meta, fastq ] } - .groupTuple(by: [0]) - .branch { - meta, fastq -> + [meta, fastq]} + .groupTuple(by: [0]) + .branch{ meta, fastq -> single : fastq.size() == 1 - return [ meta, fastq.flatten() ] + return [meta, fastq.flatten()] multiple: fastq.size() > 1 - return [ meta, fastq.flatten() ] - } - .set { ch_fastq } + return [meta, fastq.flatten()]} + .set{ch_fastq} // // MODULE: Run FastQC @@ -187,20 +181,16 @@ workflow RNAVAR { // MODULE: Concatenate FastQ files from same sample if required // - CAT_FASTQ ( - ch_fastq.multiple - ) - .mix(ch_fastq.single) - .set { ch_cat_fastq } + CAT_FASTQ(ch_fastq.multiple) + .mix(ch_fastq.single) + .set{ch_cat_fastq} // PREPARE THE INTERVAL LIST ch_interval_list = Channel.empty() - GATK4_BEDTOINTERVALLIST( - ch_genome_gtf, - PREPARE_GENOME.out.dict - ) + GATK4_BEDTOINTERVALLIST(ch_genome_gtf, PREPARE_GENOME.out.dict) ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list - ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) + ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) + // // SUBWORKFLOW: Alignment with STAR // @@ -226,61 +216,48 @@ workflow RNAVAR { ch_samtools_flagstat = ALIGN_STAR.out.flagstat ch_samtools_idxstats = ALIGN_STAR.out.idxstats ch_star_multiqc = ALIGN_STAR.out.log_final - if (params.bam_csi_index) { - ch_genome_bam_index = ALIGN_STAR.out.csi - } + if (params.bam_csi_index) ch_genome_bam_index = ALIGN_STAR.out.csi ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) // SUBWORKFLOW: Mark duplicates with Picard ch_markduplicates_multiqc = Channel.empty() if (!params.skip_alignment && !params.skip_markduplicates) { - MARK_DUPLICATES_PICARD ( - ch_genome_bam - ) - ch_genome_bam = MARK_DUPLICATES_PICARD.out.bam - ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.bai - ch_samtools_stats = MARK_DUPLICATES_PICARD.out.stats - ch_samtools_flagstat = MARK_DUPLICATES_PICARD.out.flagstat - ch_samtools_idxstats = MARK_DUPLICATES_PICARD.out.idxstats - ch_markduplicates_multiqc = MARK_DUPLICATES_PICARD.out.metrics - if (params.bam_csi_index) { - ch_genome_bam_index = MARK_DUPLICATES_PICARD.out.csi - } - ch_software_versions = ch_software_versions.mix(MARK_DUPLICATES_PICARD.out.picard_version.first().ifEmpty(null)) + MARKDUPLICATES(ch_genome_bam) + ch_genome_bam = MARKDUPLICATES.out.bam + ch_genome_bam_index = MARKDUPLICATES.out.bai + ch_samtools_stats = MARKDUPLICATES.out.stats + ch_samtools_flagstat = MARKDUPLICATES.out.flagstat + ch_samtools_idxstats = MARKDUPLICATES.out.idxstats + ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics + if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi + ch_software_versions = ch_software_versions.mix(MARKDUPLICATES.out.picard_version.first().ifEmpty(null)) } // MODULE: SplitNCigarReads from GATK4 // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() - if(!params.skip_splitncigar){ - - splitncigarreads_reference = PREPARE_GENOME.out.fasta.join(PREPARE_GENOME.out.fai).join(PREPARE_GENOME.out.dict) - - GATK4_SPLITNCIGARREADS ( - ch_genome_bam, - splitncigarreads_reference - ) - - SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) - - ch_genome_bam = GATK4_SPLITNCIGARREADS.out.bam - ch_genome_bam_index = SAMTOOLS_INDEX.out.bai - ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) - bam_splitncigar = ch_genome_bam.join(ch_genome_bam_index, by: [0]) + if (!params.skip_splitncigar) { + GATK4_SPLITNCIGARREADS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) + bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam + ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) } ch_bqsr_table = Channel.empty() - if(!params.skip_basecalibrator){ + if (!params.skip_basecalibrator) { + known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() + known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() + + ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] } GATK4_BASERECALIBRATOR( bam_splitncigar, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, - ch_interval_list, - params.dbsnp_vcf.join(params.known_indels), - params.dbsnp_vcf_index.join(params.known_indels_index) + ch_interval_list_baserecalibrator, + known_sites, + known_sites_tbi ) ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table } @@ -290,8 +267,7 @@ workflow RNAVAR { bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() - - if(!params.skip_applybqsr){ + if (!params.skip_applybqsr) { RECALIBRATE( ('bamqc' in params.skip_qc), ('samtools' in params.skip_qc), @@ -309,31 +285,26 @@ workflow RNAVAR { ch_interval_list_split = Channel.empty() - if(!params.skip_intervallisttools){ - GATK4_INTERVALLISTTOOLS( - ch_interval_list - ) + if (!params.skip_intervallisttools) { + GATK4_INTERVALLISTTOOLS(ch_interval_list) ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.flatten() } - else{ - ch_interval_list_split = ch_interval_list - } + else ch_interval_list_split = ch_interval_list interval_flag = false haplotypecaller_vcf = Channel.empty() - if(!params.skip_haplotypecaller){ + if (!params.skip_haplotypecaller) { haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) - bam_recalibrated.combine(ch_interval_list_split).map{ meta, bam, bai, interval_list -> - [meta, bam, bai, interval_list] - new_meta = meta.clone() - new_meta.id = meta.id + "_" + interval_list.baseName - [new_meta, bam, bai, interval_list] - } - .set{haplotypecaller_interval_bam} + bam_recalibrated.combine(ch_interval_list_split) + .map{ meta, bam, bai, interval_list -> + new_meta = meta.clone() + new_meta.id = meta.id + "_" + interval_list.baseName + [new_meta, bam, bai, interval_list]} + .set{haplotypecaller_interval_bam} GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, @@ -345,10 +316,11 @@ workflow RNAVAR { interval_flag ) - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf.map{ meta,vcf -> - meta.id = meta.sample - [meta, vcf] - }.groupTuple() + haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf + .map{ meta,vcf -> + meta.id = meta.sample + [meta, vcf]} + .groupTuple() use_ref_dict = true GATK4_MERGEVCFS( @@ -360,7 +332,7 @@ workflow RNAVAR { haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf } - if(!params.skip_variantfiltration){ + if (!params.skip_variantfiltration) { GATK4_VARIANTFILTRATION( haplotypecaller_vcf, @@ -370,7 +342,6 @@ workflow RNAVAR { ) filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf - } } @@ -379,16 +350,14 @@ workflow RNAVAR { // MODULE: Pipeline reporting // ch_software_versions - .map { it -> if (it) [ it.baseName, it ] } + .map{it -> if (it) [it.baseName, it]} .groupTuple() - .map { it[1][0] } + .map{it[1][0]} .flatten() .collect() - .set { ch_software_versions } + .set{ch_software_versions} - GET_SOFTWARE_VERSIONS ( - ch_software_versions.map { it }.collect() - ) + GET_SOFTWARE_VERSIONS(ch_software_versions.map{it}.collect()) // // MODULE: MultiQC @@ -403,9 +372,7 @@ workflow RNAVAR { ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - MULTIQC ( - ch_multiqc_files.collect() - ) + MULTIQC (ch_multiqc_files.collect()) multiqc_report = MULTIQC.out.report.toList() ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) } From 086ccdf6d110655c50cc57d6fee5b4d1e63e5385 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Thu, 29 Jul 2021 16:18:17 +0200 Subject: [PATCH 030/235] fix: move module to local --- modules.json | 3 --- 1 file changed, 3 deletions(-) diff --git a/modules.json b/modules.json index 89081642..21dfdf61 100644 --- a/modules.json +++ b/modules.json @@ -24,9 +24,6 @@ "gatk4/mergevcfs": { "git_sha": "e7be649029b97d12ddf33baec8e85e141f3ece1c" }, - "gatk4/splitncigarreads": { - "git_sha": "b3c466773207002060efbeff384b6f03ec0d7d9f" - }, "gatk4/variantfiltration": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, From 1f0c3902cdd100524f7ea8c426ff40ef6b34da5c Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 11:26:41 +0200 Subject: [PATCH 031/235] fix: add PL to star align --- modules.json | 4 ++-- modules/nf-core/modules/star/align/main.nf | 11 ++++++----- subworkflows/nf-core/align_star.nf | 2 +- 3 files changed, 9 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index 21dfdf61..e46617d5 100644 --- a/modules.json +++ b/modules.json @@ -76,7 +76,7 @@ "git_sha": "c5235a983d454787fa0c3247b02086969217163b" }, "star/align": { - "git_sha": "df909015b6651dadb52626cf471f15a6d33985a2" + "git_sha": "5de3f2c50e063bda30316342b6606b485a12e38c" }, "star/genomegenerate": { "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc" @@ -86,4 +86,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf index c06daf24..d5d88ce8 100644 --- a/modules/nf-core/modules/star/align/main.nf +++ b/modules/nf-core/modules/star/align/main.nf @@ -39,11 +39,12 @@ process STAR_ALIGN { tuple val(meta), path('*.out.junction') , optional:true, emit: junction script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" - def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" + def seq_platform = params.seq_platform ? "'PL:$params.seq_platform'" : "" + def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform " + def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' """ STAR \\ diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index 40cc68b6..b704a2d5 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -7,7 +7,7 @@ params.samtools_sort_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options ) +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: 'illumina') include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) workflow ALIGN_STAR { From 3b0e3ef94fb7fa116faa3ab1332e24c34198e2aa Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 11:28:30 +0200 Subject: [PATCH 032/235] fix: EClint --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index e46617d5..900d34c6 100644 --- a/modules.json +++ b/modules.json @@ -86,4 +86,4 @@ } } } -} \ No newline at end of file +} From 6f22458dad1647a58830d587aa9a88f7949faa5e Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 13:10:32 +0200 Subject: [PATCH 033/235] fix: move modules to local --- conf/modules.config | 6 +++--- modules/local/gatk4/haplotypecaller/main.nf | 2 +- .../gatk4/mergevcfs/functions.nf | 0 .../modules => local}/gatk4/mergevcfs/main.nf | 4 ++-- .../modules => local}/gatk4/mergevcfs/meta.yml | 0 .../gatk4/variantfiltration/functions.nf | 0 .../gatk4/variantfiltration/main.nf | 2 +- .../gatk4/variantfiltration/meta.yml | 0 nextflow.config | 1 + subworkflows/nf-core/align_star.nf | 5 +++-- workflows/rnavar.nf | 16 ++++++++-------- 11 files changed, 19 insertions(+), 17 deletions(-) rename modules/{nf-core/modules => local}/gatk4/mergevcfs/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/mergevcfs/main.nf (90%) rename modules/{nf-core/modules => local}/gatk4/mergevcfs/meta.yml (100%) rename modules/{nf-core/modules => local}/gatk4/variantfiltration/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/variantfiltration/main.nf (96%) rename modules/{nf-core/modules => local}/gatk4/variantfiltration/meta.yml (100%) diff --git a/conf/modules.config b/conf/modules.config index cd3e6761..022834d4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -92,7 +92,7 @@ params { publish_files = false } 'merge_bam_recalibrate' { - suffix = '.recal' + suffix = '.recal.merge' publish_by_meta = true publish_files = ['bam':'recalibrated'] publish_dir = 'preprocessing' @@ -113,14 +113,14 @@ params { publish_dir = 'reports/samtools_stats' } 'gatk_mergevcfs' { - suffix = 'haplotypecaller' + suffix = '.haplotypecaller' publish_by_meta = true publish_dir = 'variant_calling' publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] } 'gatk_variantfilter' { args = '' - suffix = 'filtered' + suffix = '.filtered' publish_by_meta = true publish_dir = 'variant_calling' publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf index 3a775e6c..da90ba4b 100644 --- a/modules/local/gatk4/haplotypecaller/main.nf +++ b/modules/local/gatk4/haplotypecaller/main.nf @@ -42,7 +42,7 @@ process GATK4_HAPLOTYPECALLER { avail_mem = task.memory.giga } def intervalsOptions = no_intervals ? "" : "-L ${interval}" - def dbsnpOptions = params.dbsnp ? "-D ${dbsnp}" : "" + def dbsnpOptions = dbsnp ? "-D ${dbsnp}" : "" //TODO allow ploidy argument here since we allow it for the cnv callers? or is this covered with options? Might unintuitive to use """ gatk \\ diff --git a/modules/nf-core/modules/gatk4/mergevcfs/functions.nf b/modules/local/gatk4/mergevcfs/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/mergevcfs/functions.nf rename to modules/local/gatk4/mergevcfs/functions.nf diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/local/gatk4/mergevcfs/main.nf similarity index 90% rename from modules/nf-core/modules/gatk4/mergevcfs/main.nf rename to modules/local/gatk4/mergevcfs/main.nf index 5a80c9ff..8b9747bc 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/local/gatk4/mergevcfs/main.nf @@ -24,8 +24,8 @@ process GATK4_MERGEVCFS { val use_ref_dict output: - tuple val(meta), path('*.vcf.gz'), emit: vcf - path '*.version.txt' , emit: version + tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi'), emit: vcf + path '*.version.txt' , emit: version script: def software = getSoftwareName(task.process) diff --git a/modules/nf-core/modules/gatk4/mergevcfs/meta.yml b/modules/local/gatk4/mergevcfs/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/mergevcfs/meta.yml rename to modules/local/gatk4/mergevcfs/meta.yml diff --git a/modules/nf-core/modules/gatk4/variantfiltration/functions.nf b/modules/local/gatk4/variantfiltration/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/variantfiltration/functions.nf rename to modules/local/gatk4/variantfiltration/functions.nf diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/local/gatk4/variantfiltration/main.nf similarity index 96% rename from modules/nf-core/modules/gatk4/variantfiltration/main.nf rename to modules/local/gatk4/variantfiltration/main.nf index 82593d18..d442aba8 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/local/gatk4/variantfiltration/main.nf @@ -19,7 +19,7 @@ process GATK4_VARIANTFILTRATION { } input: - tuple val(meta), path(vcf) + tuple val(meta), path(vcf), path (tbi) path fasta path fai path dict diff --git a/modules/nf-core/modules/gatk4/variantfiltration/meta.yml b/modules/local/gatk4/variantfiltration/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/variantfiltration/meta.yml rename to modules/local/gatk4/variantfiltration/meta.yml diff --git a/nextflow.config b/nextflow.config index 58ee6bc2..6cf5c397 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,6 +22,7 @@ params { aligner = 'star' star_ignore_sjdbgtf = false seq_center = null + seq_platform = 'illumina' save_merged_fastq = false save_unaligned = false save_align_intermeds = false diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index b704a2d5..85fbd453 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -3,11 +3,12 @@ // params.align_options = [:] -params.samtools_sort_options = [:] params.samtools_index_options = [:] +params.samtools_sort_options = [:] params.samtools_stats_options = [:] +params.seq_platform = [:] -include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: 'illumina') +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: params.seq_platform) include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) workflow ALIGN_STAR { diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index fea00dec..f0a78685 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -119,9 +119,9 @@ include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baser include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: [:]) include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) -include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) +include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) include { GATK4_SPLITNCIGARREADS } from '../modules/local/gatk4/splitncigarreads/main' addParams(options: modules['gatk_splitncigar_options']) -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) +include { GATK4_VARIANTFILTRATION } from '../modules/local/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( @@ -133,7 +133,8 @@ include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' align_options: modules['star_align'], samtools_index_options: modules['samtools_index_genome'], samtools_sort_options: modules['samtools_sort_genome'], - samtools_stats_options: modules['samtools_index_genome'] + samtools_stats_options: modules['samtools_index_genome'], + seq_platform: params.seq_platform ) include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( markduplicates_options: modules['picard_markduplicates'], @@ -268,6 +269,8 @@ workflow RNAVAR { bam_recalibrated_qc = Channel.empty() if (!params.skip_applybqsr) { + + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] } RECALIBRATE( ('bamqc' in params.skip_qc), ('samtools' in params.skip_qc), @@ -275,7 +278,7 @@ workflow RNAVAR { PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.fasta, - ch_interval_list + ch_interval_list_applybqsr ) bam_recalibrated = RECALIBRATE.out.bam @@ -287,7 +290,7 @@ workflow RNAVAR { if (!params.skip_intervallisttools) { GATK4_INTERVALLISTTOOLS(ch_interval_list) - ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.flatten() + ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() } else ch_interval_list_split = ch_interval_list @@ -298,13 +301,10 @@ workflow RNAVAR { if (!params.skip_haplotypecaller) { haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) - - bam_recalibrated.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() new_meta.id = meta.id + "_" + interval_list.baseName [new_meta, bam, bai, interval_list]} - .set{haplotypecaller_interval_bam} GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, From 69012c5af9b4125a97d9cf494be4fe744262da91 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 15:55:06 +0200 Subject: [PATCH 034/235] fix: CI should be ok now --- .nf-core.yml | 10 ++-------- nextflow.config | 4 ++-- nextflow_schema.json | 8 ++++---- 3 files changed, 8 insertions(+), 14 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index f50d6f04..239592c3 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,8 +1,2 @@ -files_unchanged: - - .github/ISSUE_TEMPLATE/bug_report.md - - .github/ISSUE_TEMPLATE/feature_request.md - - .github/PULL_REQUEST_TEMPLATE.md - - .gitignore - - assets/nf-core-rnavar_logo.png - - docs/images/nf-core-rnavar_logo.png - - lib/NfcoreSchema.groovy +lint: + files_unchanged: diff --git a/nextflow.config b/nextflow.config index 6cf5c397..946a7c3e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -43,8 +43,8 @@ params { //GATK variant filter parameters window = 35 cluster = 3 - fs_filter = 30 - qd_filter = 2 + fs_filter = 30.0 + qd_filter = 2.0 // QC skip_qc = false diff --git a/nextflow_schema.json b/nextflow_schema.json index b2c589a9..960afc1e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -351,12 +351,12 @@ "default": 3 }, "fs_filter": { - "type": "integer", - "default": 30 + "type": "number", + "default": 30.0 }, "qd_filter": { - "type": "integer", - "default": 2 + "type": "number", + "default": 2.0 }, "skip_bigwig": { "type": "string" From e006c7f3be25f8b69d3ce6b4f468ce799ed204dd Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 15:57:20 +0200 Subject: [PATCH 035/235] fix: remove unused file --- .nf-core.yml | 2 -- 1 file changed, 2 deletions(-) delete mode 100644 .nf-core.yml diff --git a/.nf-core.yml b/.nf-core.yml deleted file mode 100644 index 239592c3..00000000 --- a/.nf-core.yml +++ /dev/null @@ -1,2 +0,0 @@ -lint: - files_unchanged: From 37b83503ab71d8a1c8355b29ae3f107b18fcd37d Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 15:58:29 +0200 Subject: [PATCH 036/235] fix: update json files --- modules.json | 6 ------ nextflow_schema.json | 6 +++++- 2 files changed, 5 insertions(+), 7 deletions(-) diff --git a/modules.json b/modules.json index 900d34c6..df6c1f16 100644 --- a/modules.json +++ b/modules.json @@ -21,12 +21,6 @@ "gatk4/intervallisttools": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, - "gatk4/mergevcfs": { - "git_sha": "e7be649029b97d12ddf33baec8e85e141f3ece1c" - }, - "gatk4/variantfiltration": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, "gffread": { "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" }, diff --git a/nextflow_schema.json b/nextflow_schema.json index 960afc1e..3183aad8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -409,6 +409,10 @@ }, "star_ignore_sjdbgtf": { "type": "string" + }, + "seq_platform": { + "type": "string", + "default": "illumina" } } -} +} \ No newline at end of file From d5542f126018bfd8dfe4683b2ffefced27f535f2 Mon Sep 17 00:00:00 2001 From: MaxUlysse Date: Wed, 4 Aug 2021 15:59:39 +0200 Subject: [PATCH 037/235] fix: ECLint --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 3183aad8..25019c3d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -415,4 +415,4 @@ "default": "illumina" } } -} \ No newline at end of file +} From e56b465a23fd6a866cbee68512224ea7a674729d Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 6 Oct 2021 14:16:58 +0200 Subject: [PATCH 038/235] itest --- assets/samplesheet.csv | 3 --- assets/{samplesheet_valid.csv => samplesheet_test.csv} | 0 assets/samplesheet_valid2.csv | 3 --- assets/samplesheet_valid_mixed.csv | 3 --- conf/test.config | 2 +- 5 files changed, 1 insertion(+), 10 deletions(-) delete mode 100644 assets/samplesheet.csv rename assets/{samplesheet_valid.csv => samplesheet_test.csv} (100%) delete mode 100644 assets/samplesheet_valid2.csv delete mode 100644 assets/samplesheet_valid_mixed.csv diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv deleted file mode 100644 index 5f653ab7..00000000 --- a/assets/samplesheet.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, diff --git a/assets/samplesheet_valid.csv b/assets/samplesheet_test.csv similarity index 100% rename from assets/samplesheet_valid.csv rename to assets/samplesheet_test.csv diff --git a/assets/samplesheet_valid2.csv b/assets/samplesheet_valid2.csv deleted file mode 100644 index 955a7947..00000000 --- a/assets/samplesheet_valid2.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/assets/samplesheet_valid_mixed.csv b/assets/samplesheet_valid_mixed.csv deleted file mode 100644 index 955a7947..00000000 --- a/assets/samplesheet_valid_mixed.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/conf/test.config b/conf/test.config index 1e549bfe..de2ee1a4 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,7 @@ params { max_time = 6.h // Input data - input = 'assets/samplesheet_valid.csv' + input = 'assets/samplesheet_test.csv' // Genome references fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' From 51cd1bbee23cbe324387051766a49a4389b9e75a Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 3 Nov 2021 13:20:39 +0100 Subject: [PATCH 039/235] changes --- conf/modules.config | 49 +++---- conf/test.config | 14 -- nextflow.config | 39 +++--- nextflow_schema.json | 162 +++++++++++++---------- subworkflows/nf-core/recalibrate.nf | 4 +- workflows/rnavar.nf | 197 +++++++++++++--------------- 6 files changed, 220 insertions(+), 245 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 022834d4..b0eae76b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -29,62 +29,54 @@ params { publish_dir = 'genome/index' } 'star_align' { - args = '--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang 149' - publish_dir = "${params.aligner}" - publish_files = ['out':'log', 'tab':'log'] + args = "--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" + publish_files = false } 'samtools_sort_genome' { suffix = '.sorted' - publish_files = [:] - publish_dir = "${params.aligner}" + publish_files = false } 'samtools_index_genome' { args = '' suffix = '.sorted' - publish_files = ['stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "${params.aligner}" + publish_files = false } 'picard_markduplicates' { args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' suffix = '.markdup.sorted' publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] - publish_dir = "${params.aligner}" + publish_dir = "preprocessing" } 'picard_markduplicates_samtools' { args = '' suffix = '.markdup.sorted' publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "${params.aligner}" + publish_dir = "preprocessing" } 'gatk_splitncigar_options' { args = '' suffix = '.split_cigar' - publish_files = ['bam': '', 'bai': ''] - publish_dir = "${params.aligner}" + publish_files = false } 'gatk_baserecalibrator' { args = '' suffix = '' - publish_files = [:] - publish_dir = "${params.aligner}/recalibrated" + publish_files = false } 'gatk_applybqsr' { args = '' - suffix = '' - publish_files = [:] - publish_dir = "${params.aligner}/recalibrated" + suffix = '.markdup.recalibrated' + publish_files = false } 'gatk_haplotypecaller' { args = '-dont-use-soft-clipped-bases --standard-min-confidence-threshold-for-calling 20' suffix = '' - publish_files = [:] - publish_dir = "${params.aligner}/variants" + publish_files = false } 'gatk_intervallisttools' { args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' suffix = '' - publish_files = [:] - publish_dir = "${params.aligner}/interval_lists" + publish_files = false } // RECALIBRATE 'applybqsr' { @@ -94,19 +86,14 @@ params { 'merge_bam_recalibrate' { suffix = '.recal.merge' publish_by_meta = true - publish_files = ['bam':'recalibrated'] + publish_files = ['bam':'', 'bai':''] publish_dir = 'preprocessing' } - 'qualimap_bamqc_recalibrate' { - args = '--paint-chromosome-limits --genome-gc-distr HUMAN -skip-duplicated --skip-dup-mode 0 -outformat HTML' - publish_by_meta = true - publish_dir = 'reports/qualimap' - } 'samtools_index_recalibrate' { - suffix = 'recal' + suffix = 'recalibrated' publish_by_meta = true publish_dir = 'preprocessing' - publish_files = ['recal.bam':'recalibrated', 'recal.bam.bai':'recalibrated'] + publish_files = ['bam':'', 'bai':''] } 'samtools_stats_recalibrate' { publish_by_meta = true @@ -116,14 +103,14 @@ params { suffix = '.haplotypecaller' publish_by_meta = true publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] } 'gatk_variantfilter' { args = '' suffix = '.filtered' publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'haplotypecaller', 'vcf.gz.tbi':'haplotypecaller'] + publish_dir = 'variant_filtering' + publish_files = ['vcf':'', 'vcf.idx':''] } 'fastqc' { args = "--quiet" diff --git a/conf/test.config b/conf/test.config index de2ee1a4..a47ea439 100644 --- a/conf/test.config +++ b/conf/test.config @@ -35,19 +35,5 @@ params { known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' - hisat2_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/hisat2.tar.gz' star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' - salmon_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/salmon.tar.gz' - rsem_index = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/rsem.tar.gz' - - // Other parameters - pseudo_aligner = 'salmon' - umitools_bc_pattern = 'NNNN' - - // When using RSEM, remove warning from STAR whilst building tiny indices - modules { - 'rsem_preparereference' { - args2 = "--genomeSAindexNbases 7" - } - } } diff --git a/nextflow.config b/nextflow.config index 946a7c3e..7ce1392e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,28 +17,35 @@ params { igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_ignore = false save_reference = false + save_merged_fastq = false + gene_bed = null + read_length = 150 // Alignment aligner = 'star' + star_index = null star_ignore_sjdbgtf = false seq_center = null seq_platform = 'illumina' - save_merged_fastq = false + bam_csi_index = false save_unaligned = false save_align_intermeds = false - skip_markduplicates = false - skip_alignment = false - skip_splitncigar = false - skip_basecalibrator = false - skip_applybqsr = false - skip_haplotypecaller = false + + //Known variant references + known_indels = null + known_indels_index = null + dbsnp_vcf = null + dbsnp_vcf_index = null + + // Variant calling + no_intervals = false + + // Skip steps skip_intervallisttools = false skip_variantfiltration = false - // GATK parameters + // GATK intervallist parameters scatter_count = 6 - no_intervals = false - skip_qc = null //GATK variant filter parameters window = 35 @@ -48,18 +55,6 @@ params { // QC skip_qc = false - skip_bigwig = false - skip_stringtie = false - skip_fastqc = false - skip_preseq = false - skip_dupradar = false - skip_qualimap = false - skip_rseqc = false - skip_biotype_qc = false - skip_deseq2_qc = false - skip_multiqc = false - deseq2_vst = false - rseqc_modules = 'bam_stat,inner_distance,infer_experiment,junction_annotation,junction_saturation,read_distribution,read_duplication' // MultiQC options multiqc_config = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 25019c3d..5ece5a88 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -62,7 +62,7 @@ "mimetype": "text/plain", "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a STAR index available, this will be generated for you automatically. Combine with `--save_reference` to save STAR index for future runs.", "fa_icon": "far fa-file-code" }, "igenomes_base": { @@ -71,7 +71,8 @@ "description": "Directory / URL base for iGenomes references.", "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", - "hidden": true + "hidden": true, + "help_text": "This parameter is to set the directory or the URL base for iGenomes references." }, "igenomes_ignore": { "type": "boolean", @@ -290,129 +291,154 @@ ], "properties": { "save_reference": { - "type": "string" + "type": "string", + "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", + "description": "If generated by the pipeline, save the STAR index in the results directory." }, "aligner": { "type": "string", - "default": "star" + "default": "star", + "description": "Specifies the alignment algorithm to use. Currently available option is 'star'", + "help_text": "This parameter define which aligner is to be used for aligning the RNA reads to the reference genome. Currently only STAR aligner is supported. So use 'star' as the value for this option." }, "seq_center": { - "type": "string" + "type": "string", + "description": "Sequencing center information to be added to read group of BAM files.", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " }, "save_merged_fastq": { - "type": "string" + "type": "string", + "description": "Save FastQ files after merging re-sequenced libraries in the results directory." }, "save_unaligned": { - "type": "string" + "type": "string", + "description": "Where possible, save unaligned reads from aligner to the results directory.", + "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, "save_align_intermeds": { - "type": "string" + "type": "string", + "description": "Save the intermediate BAM files from the alignment step.", + "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." }, "skip_markduplicates": { - "type": "string" + "type": "string", + "description": "Skip picard MarkDuplicates step.", + "help_text": "Set this command to skip the mark_duplicate step by GATK" }, "skip_alignment": { - "type": "string" + "type": "string", + "description": "Skip all of the alignment-based processes within the pipeline." }, "skip_splitncigar": { - "type": "string" + "type": "string", + "description": "Skip the SplitNCigar module of GATK within the pipeline.", + "help_text": "This parameter is to avoid the SplitNCigar module of GATK within the pipeline. It is recommended to use this step for RNAseq data. More details can be found in [GATK documentation](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads)" }, "skip_basecalibrator": { - "type": "string" + "type": "string", + "description": "Skip the base-recalibration step in GATK pipeline", + "help_text": "This parameter is to disable the base-recalibration step in GATK pipeline" }, "skip_applybqsr": { - "type": "string" + "type": "string", + "description": "Skip applying the reclaibrated qualities to the reads", + "help_text": "This parameter is to skip applying the recalibrated base qualities to the reads. If set, no recalibrated BAM is created." }, "skip_haplotypecaller": { - "type": "string" + "type": "string", + "description": "Skip the variant calling step", + "help_text": "This parameter is to disable the variant calling step in the GATK. The module uses HaplotypeCaller (HC) to call variants and if this parameter is set, HaplotypeCaller will not run and no variants (VCF) files will be produced." }, "skip_intervallisttools": { - "type": "string" + "type": "string", + "description": "Skip the process of preparing interval lists for the GATK variant calling step", + "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." }, "skip_variantfiltration": { - "type": "string" + "type": "string", + "description": "Skip variant filtering of GATK", + "help_text": "Set this parameter if you don't want to filter any variants. " }, "scatter_count": { "type": "integer", - "default": 6 + "default": 6, + "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", + "help_text": "Set this parameter to decide the number of splits for the gene interval list file." }, "no_intervals": { - "type": "string" + "type": "string", + "description": "Do not use gene interval file during variant calling", + "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", + "default": "False" }, "skip_qc": { - "type": "string" + "type": "string", + "description": "Skip QC reports", + "help_text": "This parameter disable all QC reports from recalibrated BAM file." }, "window": { "type": "integer", - "default": 35 + "default": 35, + "description": "The window size (in bases) in which to evaluate clustered SNPs.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." }, "cluster": { "type": "integer", - "default": 3 + "default": 3, + "description": "The number of SNPs which make up a cluster. Must be at least 2.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." }, "fs_filter": { "type": "number", - "default": 30.0 + "default": 30, + "description": "Value to be used for the FisherStrand (FS) filter", + "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" }, "qd_filter": { "type": "number", - "default": 2.0 - }, - "skip_bigwig": { - "type": "string" - }, - "skip_stringtie": { - "type": "string" - }, - "skip_fastqc": { - "type": "string" - }, - "skip_preseq": { - "type": "string" - }, - "skip_dupradar": { - "type": "string" - }, - "skip_qualimap": { - "type": "string" - }, - "skip_rseqc": { - "type": "string" - }, - "skip_biotype_qc": { - "type": "string" - }, - "skip_deseq2_qc": { - "type": "string" - }, - "skip_multiqc": { - "type": "string" - }, - "deseq2_vst": { - "type": "string" - }, - "rseqc_modules": { - "type": "string", - "default": "bam_stat,inner_distance,infer_experiment,junction_annotation,junction_saturation,read_distribution,read_duplication" + "default": 2, + "description": "Value to be used for the QualByDepth (QD) filter", + "help_text": "This parameter defines the value to use for the QualByDepth (QD) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 2.0" }, "gtf": { - "type": "string" + "type": "string", + "description": "Path to GTF annotation file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified." }, "gff": { - "type": "string" + "type": "string", + "description": "Path to GFF3 annotation file.", + "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." }, "gene_bed": { - "type": "string" + "type": "string", + "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." }, "star_index": { - "type": "string" + "type": "string", + "description": "Path to STAR index folder or compressed file (tar.gz)", + "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." }, "star_ignore_sjdbgtf": { - "type": "string" + "type": "string", + "description": "Do not use GTF file during STAR index buidling step", + "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." }, "seq_platform": { "type": "string", - "default": "illumina" + "default": "illumina", + "description": "Specify the sequencing platform used", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " + }, + "read_length": { + "type": "number", + "default": 150, + "description": "Read length", + "help_text": "Specify the read length for the STAR aligner." } - } -} + }, + "required": [ + "aligner", + "seq_platform" + ] +} \ No newline at end of file diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 61f4459d..9cfccca5 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -11,7 +11,6 @@ params.samtools_index_options = [:] params.samtools_stats_options = [:] include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/local/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) -include { QUALIMAP_BAMQC } from '../../modules/nf-core/modules/qualimap/bamqc/main' addParams(options: params.qualimap_bamqc_options) include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) @@ -55,14 +54,13 @@ workflow RECALIBRATE { SAMTOOLS_INDEX(bam_recalibrated) bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) - qualimap_bamqc = Channel.empty() samtools_stats = Channel.empty() if (!skip_samtools) { SAMTOOLS_STATS(bam_recalibrated_index) samtools_stats = SAMTOOLS_STATS.out.stats } - bam_reports = samtools_stats.mix(qualimap_bamqc) + bam_reports = samtools_stats } emit: diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index b5e3bffd..d8130a55 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -9,14 +9,15 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowRnavar.initialise(params, log) -// TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist def checkPathParamList = [ params.input, params.fasta, params.gtf, - params.gff, - params.gene_bed, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + params.known_indels, + params.known_indels_index, params.star_index] for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} @@ -75,7 +76,7 @@ include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams(opt // Check alignment parameters def prepareToolIndices = [] -if (!params.skip_alignment) { prepareToolIndices = params.aligner } +prepareToolIndices = params.aligner def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] @@ -84,18 +85,10 @@ if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = fa modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -if (['star'].contains(params.aligner)) { - if (params.save_align_intermeds || (!params.with_umi && params.skip_markduplicates)) { - modules['samtools_sort_genome'].publish_files.put('bam','') - modules['samtools_index_genome'].publish_files.put('bai','') - modules['samtools_index_genome'].publish_files.put('csi','') - } -} else { - if (params.save_align_intermeds || params.skip_markduplicates) { - modules['samtools_sort_genome'].publish_files.put('bam','') - modules['samtools_index_genome'].publish_files.put('bai','') - modules['samtools_index_genome'].publish_files.put('csi','') - } +if (params.save_align_intermeds) { + modules['samtools_sort_genome'].publish_files.put('bam','') + modules['samtools_index_genome'].publish_files.put('bai','') + modules['samtools_index_genome'].publish_files.put('csi','') } def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type @@ -144,7 +137,6 @@ include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( applybqsr_options: modules['applybqsr'], merge_bam_options: modules['merge_bam_recalibrate'], - qualimap_bamqc_options: modules['qualimap_bamqc_recalibrate'], samtools_index_options: modules['samtools_index_recalibrate'], samtools_stats_options: modules['samtools_stats_recalibrate'] ) @@ -174,19 +166,17 @@ workflow RNAVAR { return [meta, fastq.flatten()]} .set{ch_fastq} - // - // MODULE: Run FastQC - // - // FASTQC(ch_fastq) - // ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - // MODULE: Concatenate FastQ files from same sample if required - // CAT_FASTQ(ch_fastq.multiple) .mix(ch_fastq.single) .set{ch_cat_fastq} - // PREPARE THE INTERVAL LIST + // MODULE: Run FastQC + FASTQC(ch_cat_fastq) + ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + + + // PREPARE THE INTERVAL LIST FROM GTF FILE ch_interval_list = Channel.empty() GATK4_BEDTOINTERVALLIST(ch_genome_gtf, PREPARE_GENOME.out.dict) ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list @@ -204,7 +194,7 @@ workflow RNAVAR { ch_aligner_pca_multiqc = Channel.empty() ch_aligner_clustering_multiqc = Channel.empty() - if (!params.skip_alignment && params.aligner == 'star') { + if (params.aligner == 'star') { ALIGN_STAR ( ch_cat_fastq, PREPARE_GENOME.out.star_index, @@ -222,70 +212,63 @@ workflow RNAVAR { ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) // SUBWORKFLOW: Mark duplicates with Picard - ch_markduplicates_multiqc = Channel.empty() - if (!params.skip_alignment && !params.skip_markduplicates) { - MARKDUPLICATES(ch_genome_bam) - ch_genome_bam = MARKDUPLICATES.out.bam - ch_genome_bam_index = MARKDUPLICATES.out.bai - ch_samtools_stats = MARKDUPLICATES.out.stats - ch_samtools_flagstat = MARKDUPLICATES.out.flagstat - ch_samtools_idxstats = MARKDUPLICATES.out.idxstats - ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics - if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi - ch_software_versions = ch_software_versions.mix(MARKDUPLICATES.out.picard_version.first().ifEmpty(null)) - } + MARKDUPLICATES(ch_genome_bam) + ch_genome_bam = MARKDUPLICATES.out.bam + ch_genome_bam_index = MARKDUPLICATES.out.bai + ch_samtools_stats = MARKDUPLICATES.out.stats + ch_samtools_flagstat = MARKDUPLICATES.out.flagstat + ch_samtools_idxstats = MARKDUPLICATES.out.idxstats + ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics + if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi + ch_software_versions = ch_software_versions.mix(MARKDUPLICATES.out.picard_version.first().ifEmpty(null)) + // MODULE: SplitNCigarReads from GATK4 // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() - if (!params.skip_splitncigar) { - GATK4_SPLITNCIGARREADS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) - bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam - ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) - } + GATK4_SPLITNCIGARREADS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) + bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam + ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) ch_bqsr_table = Channel.empty() - if (!params.skip_basecalibrator) { - known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() - known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() + // MODULE: BaseRecalibrator from GATK4 + known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() + known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() - ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] } + ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] } - GATK4_BASERECALIBRATOR( - bam_splitncigar, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - ch_interval_list_baserecalibrator, - known_sites, - known_sites_tbi - ) - ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table - } + GATK4_BASERECALIBRATOR( + bam_splitncigar, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list_baserecalibrator, + known_sites, + known_sites_tbi + ) + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table - bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) + // MODULE: ApplyBaseRecalibrator from GATK4 + bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() - if (!params.skip_applybqsr) { - - ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] } - RECALIBRATE( - ('bamqc' in params.skip_qc), - ('samtools' in params.skip_qc), - bam_applybqsr, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.fasta, - ch_interval_list_applybqsr - ) - - bam_recalibrated = RECALIBRATE.out.bam - bam_recalibrated_qc = RECALIBRATE.out.qc + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] } + RECALIBRATE( + ('bamqc' in params.skip_qc), + ('samtools' in params.skip_qc), + bam_applybqsr, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.fasta, + ch_interval_list_applybqsr + ) - } + bam_recalibrated = RECALIBRATE.out.bam + bam_recalibrated_qc = RECALIBRATE.out.qc + // MODULE: IntervalListTools from GATK4 ch_interval_list_split = Channel.empty() if (!params.skip_intervallisttools) { @@ -294,43 +277,43 @@ workflow RNAVAR { } else ch_interval_list_split = ch_interval_list - interval_flag = false + // MODULE: HaplotypeCaller from GATK4 + interval_flag = false haplotypecaller_vcf = Channel.empty() - if (!params.skip_haplotypecaller) { - - haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) - .map{ meta, bam, bai, interval_list -> - new_meta = meta.clone() - new_meta.id = meta.id + "_" + interval_list.baseName - [new_meta, bam, bai, interval_list]} + haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) + .map{ meta, bam, bai, interval_list -> + new_meta = meta.clone() + new_meta.id = meta.id + "_" + interval_list.baseName + [new_meta, bam, bai, interval_list]} + + GATK4_HAPLOTYPECALLER( + haplotypecaller_interval_bam, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + interval_flag + ) - GATK4_HAPLOTYPECALLER( - haplotypecaller_interval_bam, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - interval_flag - ) + haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf + .map{ meta,vcf -> + meta.id = meta.sample + [meta, vcf]} + .groupTuple() + + use_ref_dict = true + GATK4_MERGEVCFS( + haplotypecaller_raw, + PREPARE_GENOME.out.dict, + use_ref_dict + ) - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf - .map{ meta,vcf -> - meta.id = meta.sample - [meta, vcf]} - .groupTuple() - - use_ref_dict = true - GATK4_MERGEVCFS( - haplotypecaller_raw, - PREPARE_GENOME.out.dict, - use_ref_dict - ) + haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf - haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf - } + // MODULE: VariantFiltration from GATK4 if (!params.skip_variantfiltration) { @@ -370,7 +353,7 @@ workflow RNAVAR { ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - // ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) MULTIQC (ch_multiqc_files.collect()) multiqc_report = MULTIQC.out.report.toList() From c3d4571b566749e989fd501d9f1b65aeb5a4b727 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 10 Nov 2021 12:05:45 +0100 Subject: [PATCH 040/235] Fix: warnings, lint errors, code cleanup, docs --- conf/modules.config | 16 +- conf/test.config | 7 +- docs/output.md | 2 - docs/usage.md | 2 - modules/local/cat_additional_fasta.nf | 39 --- modules/local/get_chrom_sizes.nf | 6 +- nextflow.config | 22 +- nextflow_schema.json | 333 ++++++++++++++------------ subworkflows/local/prepare_genome.nf | 117 +-------- workflows/rnavar.nf | 25 +- 10 files changed, 237 insertions(+), 332 deletions(-) delete mode 100644 modules/local/cat_additional_fasta.nf diff --git a/conf/modules.config b/conf/modules.config index b0eae76b..865c1b44 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -26,6 +26,7 @@ params { publish_dir = 'fastq' } 'star_genomegenerate' { + args = "--sjdbOverhang ${params.read_length - 1}" publish_dir = 'genome/index' } 'star_align' { @@ -46,12 +47,14 @@ params { suffix = '.markdup.sorted' publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] publish_dir = "preprocessing" + publish_by_meta = true } 'picard_markduplicates_samtools' { args = '' suffix = '.markdup.sorted' publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] publish_dir = "preprocessing" + publish_by_meta = true } 'gatk_splitncigar_options' { args = '' @@ -78,6 +81,10 @@ params { suffix = '' publish_files = false } + 'gatk_bedtointervallist' { + args = '' + publish_files = false + } // RECALIBRATE 'applybqsr' { suffix = '.recal' @@ -113,9 +120,12 @@ params { publish_files = ['vcf':'', 'vcf.idx':''] } 'fastqc' { - args = "--quiet" + args = "--quiet" + publish_files = false } 'multiqc' { - args = "" - } } + args = "" + publish_dir = 'reports' + } + } } diff --git a/conf/test.config b/conf/test.config index a47ea439..95148324 100644 --- a/conf/test.config +++ b/conf/test.config @@ -27,13 +27,14 @@ params { fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' fasta_dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' - gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' - transcript_fasta = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/transcriptome.fasta' + //gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' + // Known genome resources (optional) dbsnp_vcf = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' dbsnp_vcf_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' - star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' + // STAR index (optional) + //star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' } diff --git a/docs/output.md b/docs/output.md index f75f868f..9890b7ea 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,8 +6,6 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: diff --git a/docs/usage.md b/docs/usage.md index 00081b8b..585a7d7f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,8 +6,6 @@ ## Introduction - - ## Samplesheet input You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. diff --git a/modules/local/cat_additional_fasta.nf b/modules/local/cat_additional_fasta.nf deleted file mode 100644 index a4fb6a11..00000000 --- a/modules/local/cat_additional_fasta.nf +++ /dev/null @@ -1,39 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process CAT_ADDITIONAL_FASTA { - tag "$add_fasta" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } - - input: - path fasta - path gtf - path add_fasta - val biotype - - output: - path "${name}.fasta", emit: fasta - path "${name}.gtf" , emit: gtf - - script: - def genome_name = params.genome ? params.genome : fasta.getBaseName() - def biotype_name = biotype ? "-b $biotype" : '' - def add_name = add_fasta.getBaseName() - name = "${genome_name}_${add_name}" - """ - fasta2gtf.py -o ${add_fasta.baseName}.gtf $biotype_name $add_fasta - cat $fasta $add_fasta > ${name}.fasta - cat $gtf ${add_fasta.baseName}.gtf > ${name}.gtf - """ -} diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf index e20fb395..56c5718c 100644 --- a/modules/local/get_chrom_sizes.nf +++ b/modules/local/get_chrom_sizes.nf @@ -9,11 +9,11 @@ process GET_CHROM_SIZES { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: diff --git a/nextflow.config b/nextflow.config index 7ce1392e..edcb06c9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,24 +9,28 @@ // Global default params, used in configs params { - // Input options - input = null + // Pipeline options - // References + // Mandatory option + input = null // sample sheet + + // Genome and reference options genome = null igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_ignore = false save_reference = false save_merged_fastq = false - gene_bed = null - read_length = 150 + gtf = null + + // Sequence read information + read_length = 150 // Required for STAR to build index and align reads // Alignment - aligner = 'star' - star_index = null - star_ignore_sjdbgtf = false + aligner = 'star' // Only STAR is currently supported. + star_index = null // Provide STAR index if there is already a pre-defined one, or set to null to create one by the pipeline + star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR seq_center = null - seq_platform = 'illumina' + seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work bam_csi_index = false save_unaligned = false save_align_intermeds = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 5ece5a88..7aa3e37e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -41,6 +41,10 @@ "type": "string", "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", "fa_icon": "fas fa-file-signature" + }, + "save_merged_fastq": { + "type": "string", + "description": "Save FastQ files after merging re-sequenced libraries in the results directory." } } }, @@ -65,6 +69,18 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a STAR index available, this will be generated for you automatically. Combine with `--save_reference` to save STAR index for future runs.", "fa_icon": "far fa-file-code" }, + "fasta_dict": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to FASTA dictionary file.", + "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference." + }, + "fasta_fai": { + "type": "string", + "fa_icon": "fas fa-file", + "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference", + "description": "Path to FASTA reference index." + }, "igenomes_base": { "type": "string", "format": "directory-path", @@ -80,6 +96,163 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "gtf": { + "type": "string", + "description": "Path to GTF annotation file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified." + }, + "gff": { + "type": "string", + "description": "Path to GFF3 annotation file.", + "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." + }, + "gene_bed": { + "type": "string", + "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." + }, + "read_length": { + "type": "number", + "default": 150, + "description": "Read length", + "help_text": "Specify the read length for the STAR aligner." + }, + "save_reference": { + "type": "string", + "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", + "description": "If generated by the pipeline, save the STAR index in the results directory." + }, + "known_indels": { + "type": "string", + "description": "Path to known indels VCF file" + }, + "known_indels_index": { + "type": "string", + "description": "Path to known indels index file" + }, + "dbsnp_vcf": { + "type": "string", + "description": "Path to dbSNP VCF file" + }, + "dbsnp_vcf_index": { + "type": "string", + "description": "Path to dbSNP VCF index file" + } + } + }, + "alignment_options": { + "title": "Alignment options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters related to read alignment", + "properties": { + "aligner": { + "type": "string", + "default": "star", + "description": "Specifies the alignment algorithm to use. Currently available option is 'star'", + "help_text": "This parameter define which aligner is to be used for aligning the RNA reads to the reference genome. Currently only STAR aligner is supported. So use 'star' as the value for this option." + }, + "star_index": { + "type": "string", + "description": "Path to STAR index folder or compressed file (tar.gz)", + "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." + }, + "star_ignore_sjdbgtf": { + "type": "string", + "description": "Do not use GTF file during STAR index buidling step", + "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." + }, + "seq_center": { + "type": "string", + "description": "Sequencing center information to be added to read group of BAM files.", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " + }, + "seq_platform": { + "type": "string", + "default": "illumina", + "description": "Specify the sequencing platform used", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " + }, + "save_unaligned": { + "type": "string", + "description": "Where possible, save unaligned reads from aligner to the results directory.", + "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." + }, + "save_align_intermeds": { + "type": "string", + "description": "Save the intermediate BAM files from the alignment step.", + "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." + }, + "bam_csi_index": { + "type": "boolean", + "description": "Create a CSI index for BAM files instead of the traditional BAI index. This will be required for genomes with larger chromosome sizes." + } + } + }, + "pipeline_stage_options": { + "title": "Pipeline stage options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters that control the stages in the pipeline", + "properties": { + "skip_intervallisttools": { + "type": "string", + "description": "Skip the process of preparing interval lists for the GATK variant calling step", + "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." + }, + "skip_variantfiltration": { + "type": "string", + "description": "Skip variant filtering of GATK", + "help_text": "Set this parameter if you don't want to filter any variants. " + }, + "skip_qc": { + "type": "string", + "description": "Skip QC reports", + "help_text": "This parameter disable all QC reports from recalibrated BAM file." + } + } + }, + "tool_parameters": { + "title": "Tool parameters", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters of the tools used in the pipeline", + "properties": { + "scatter_count": { + "type": "integer", + "default": 6, + "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", + "help_text": "Set this parameter to decide the number of splits for the gene interval list file." + }, + "no_intervals": { + "type": "string", + "description": "Do not use gene interval file during variant calling", + "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", + "default": "False" + }, + "window": { + "type": "integer", + "default": 35, + "description": "The window size (in bases) in which to evaluate clustered SNPs.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." + }, + "cluster": { + "type": "integer", + "default": 3, + "description": "The number of SNPs which make up a cluster. Must be at least 2.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." + }, + "fs_filter": { + "type": "number", + "default": 30, + "description": "Value to be used for the FisherStrand (FS) filter", + "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" + }, + "qd_filter": { + "type": "number", + "default": 2, + "description": "Value to be used for the QualByDepth (QD) filter", + "help_text": "This parameter defines the value to use for the QualByDepth (QD) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 2.0" } } }, @@ -288,155 +461,19 @@ { "$ref": "#/definitions/generic_options" } - ], - "properties": { - "save_reference": { - "type": "string", - "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", - "description": "If generated by the pipeline, save the STAR index in the results directory." - }, - "aligner": { - "type": "string", - "default": "star", - "description": "Specifies the alignment algorithm to use. Currently available option is 'star'", - "help_text": "This parameter define which aligner is to be used for aligning the RNA reads to the reference genome. Currently only STAR aligner is supported. So use 'star' as the value for this option." - }, - "seq_center": { - "type": "string", - "description": "Sequencing center information to be added to read group of BAM files.", - "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " - }, - "save_merged_fastq": { - "type": "string", - "description": "Save FastQ files after merging re-sequenced libraries in the results directory." - }, - "save_unaligned": { - "type": "string", - "description": "Where possible, save unaligned reads from aligner to the results directory.", - "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." - }, - "save_align_intermeds": { - "type": "string", - "description": "Save the intermediate BAM files from the alignment step.", - "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." - }, - "skip_markduplicates": { - "type": "string", - "description": "Skip picard MarkDuplicates step.", - "help_text": "Set this command to skip the mark_duplicate step by GATK" - }, - "skip_alignment": { - "type": "string", - "description": "Skip all of the alignment-based processes within the pipeline." - }, - "skip_splitncigar": { - "type": "string", - "description": "Skip the SplitNCigar module of GATK within the pipeline.", - "help_text": "This parameter is to avoid the SplitNCigar module of GATK within the pipeline. It is recommended to use this step for RNAseq data. More details can be found in [GATK documentation](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads)" - }, - "skip_basecalibrator": { - "type": "string", - "description": "Skip the base-recalibration step in GATK pipeline", - "help_text": "This parameter is to disable the base-recalibration step in GATK pipeline" - }, - "skip_applybqsr": { - "type": "string", - "description": "Skip applying the reclaibrated qualities to the reads", - "help_text": "This parameter is to skip applying the recalibrated base qualities to the reads. If set, no recalibrated BAM is created." - }, - "skip_haplotypecaller": { - "type": "string", - "description": "Skip the variant calling step", - "help_text": "This parameter is to disable the variant calling step in the GATK. The module uses HaplotypeCaller (HC) to call variants and if this parameter is set, HaplotypeCaller will not run and no variants (VCF) files will be produced." - }, - "skip_intervallisttools": { - "type": "string", - "description": "Skip the process of preparing interval lists for the GATK variant calling step", - "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." - }, - "skip_variantfiltration": { - "type": "string", - "description": "Skip variant filtering of GATK", - "help_text": "Set this parameter if you don't want to filter any variants. " - }, - "scatter_count": { - "type": "integer", - "default": 6, - "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", - "help_text": "Set this parameter to decide the number of splits for the gene interval list file." - }, - "no_intervals": { - "type": "string", - "description": "Do not use gene interval file during variant calling", - "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", - "default": "False" - }, - "skip_qc": { - "type": "string", - "description": "Skip QC reports", - "help_text": "This parameter disable all QC reports from recalibrated BAM file." - }, - "window": { - "type": "integer", - "default": 35, - "description": "The window size (in bases) in which to evaluate clustered SNPs.", - "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." - }, - "cluster": { - "type": "integer", - "default": 3, - "description": "The number of SNPs which make up a cluster. Must be at least 2.", - "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." - }, - "fs_filter": { - "type": "number", - "default": 30, - "description": "Value to be used for the FisherStrand (FS) filter", - "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" - }, - "qd_filter": { - "type": "number", - "default": 2, - "description": "Value to be used for the QualByDepth (QD) filter", - "help_text": "This parameter defines the value to use for the QualByDepth (QD) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 2.0" - }, - "gtf": { - "type": "string", - "description": "Path to GTF annotation file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified." - }, - "gff": { - "type": "string", - "description": "Path to GFF3 annotation file.", - "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." - }, - "gene_bed": { - "type": "string", - "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." - }, - "star_index": { - "type": "string", - "description": "Path to STAR index folder or compressed file (tar.gz)", - "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." - }, - "star_ignore_sjdbgtf": { - "type": "string", - "description": "Do not use GTF file during STAR index buidling step", - "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." - }, - "seq_platform": { - "type": "string", - "default": "illumina", - "description": "Specify the sequencing platform used", - "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " - }, - "read_length": { - "type": "number", - "default": 150, - "description": "Read length", - "help_text": "Specify the read length for the STAR aligner." + , + { + "$ref": "#/definitions/tool_parameters" } - }, + , + { + "$ref": "#/definitions/pipeline_stage_options" + } + , + { + "$ref": "#/definitions/alignment_options" + } + ], "required": [ "aligner", "seq_platform" diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 9b32a48c..f9a05d8d 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -4,41 +4,25 @@ params.genome_options = [:] params.gffread_options = [:] -params.hisat2_index_options = [:] params.index_options = [:] -params.rsem_index_options = [:] -params.salmon_index_options = [:] params.star_index_options = [:] -include { CAT_ADDITIONAL_FASTA } from '../../modules/local/cat_additional_fasta' addParams(options: params.genome_options) include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams(options: params.gffread_options) include { GTF2BED } from '../../modules/local/gtf2bed' addParams(options: params.genome_options) -include { GTF_GENE_FILTER } from '../../modules/local/gtf_gene_filter' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_ADDITIONAL_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_TRANSCRIPT_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { HISAT2_BUILD } from '../../modules/nf-core/modules/hisat2/build/main' addParams(options: params.hisat2_index_options) -include { HISAT2_EXTRACTSPLICESITES } from '../../modules/nf-core/modules/hisat2/extractsplicesites/main' addParams(options: params.hisat2_index_options) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams(options: params.rsem_index_options) -include { RSEM_PREPAREREFERENCE as RSEM_PREPAREREFERENCE_TRANSCRIPTS } from '../../modules/nf-core/modules/rsem/preparereference/main' addParams(options: params.genome_options) -include { SALMON_INDEX } from '../../modules/nf-core/modules/salmon/index/main' addParams(options: params.salmon_index_options) include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) -include { UNTAR as UNTAR_HISAT2_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.hisat2_index_options) -include { UNTAR as UNTAR_RSEM_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.index_options) -include { UNTAR as UNTAR_SALMON_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.index_options) include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_index_options) + workflow PREPARE_GENOME { take: - prepare_tool_indices // list : tools to prepare indices for - biotype // string: if additional fasta file is provided - // biotype value to use when appending entries to GTF file + prepare_tool_indices main: @@ -71,20 +55,6 @@ workflow PREPARE_GENOME { ch_gffread_version = GFFREAD.out.version } - // - // Uncompress additional fasta file and concatenate with reference fasta and gtf files - // - if (params.additional_fasta) { - if (params.additional_fasta.endsWith('.gz')) { - ch_add_fasta = GUNZIP_ADDITIONAL_FASTA ( params.additional_fasta ).gunzip - } else { - ch_add_fasta = file(params.additional_fasta) - } - CAT_ADDITIONAL_FASTA ( ch_fasta, ch_gtf, ch_add_fasta, biotype ) - ch_fasta = CAT_ADDITIONAL_FASTA.out.fasta - ch_gtf = CAT_ADDITIONAL_FASTA.out.gtf - } - // // Uncompress gene BED annotation file or create from GTF if required // @@ -98,21 +68,6 @@ workflow PREPARE_GENOME { ch_gene_bed = GTF2BED ( ch_gtf ) } - // - // Uncompress transcript fasta file / create if required - // - ch_rsem_version = Channel.empty() - if (params.transcript_fasta) { - if (params.transcript_fasta.endsWith('.gz')) { - ch_transcript_fasta = GUNZIP_TRANSCRIPT_FASTA ( params.transcript_fasta ).gunzip - } else { - ch_transcript_fasta = file(params.transcript_fasta) - } - } else { - ch_transcript_fasta = RSEM_PREPAREREFERENCE_TRANSCRIPTS ( ch_fasta, GTF_GENE_FILTER ( ch_fasta, ch_gtf ) ).transcript_fasta - ch_rsem_version = RSEM_PREPAREREFERENCE_TRANSCRIPTS.out.version - } - // Index the genome fasta ch_fasta_fai = Channel.empty() if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) @@ -146,82 +101,14 @@ workflow PREPARE_GENOME { } } - // - // Uncompress RSEM index or generate from scratch if required - // - ch_rsem_index = Channel.empty() - if ('star_rsem' in prepare_tool_indices) { - if (params.rsem_index) { - if (params.rsem_index.endsWith('.tar.gz')) { - ch_rsem_index = UNTAR_RSEM_INDEX ( params.rsem_index ).untar - } else { - ch_rsem_index = file(params.rsem_index) - } - } else { - ch_rsem_index = RSEM_PREPAREREFERENCE ( ch_fasta, ch_gtf ).index - ch_rsem_version = RSEM_PREPAREREFERENCE.out.version - } - } - - // - // Uncompress HISAT2 index or generate from scratch if required - // - ch_splicesites = Channel.empty() - ch_hisat2_index = Channel.empty() - ch_hisat2_version = Channel.empty() - if ('hisat2' in prepare_tool_indices) { - if (!params.splicesites) { - ch_splicesites = HISAT2_EXTRACTSPLICESITES ( ch_gtf ).txt - ch_hisat2_version = HISAT2_EXTRACTSPLICESITES.out.version - } else { - ch_splicesites = file(params.splicesites) - } - if (params.hisat2_index) { - if (params.hisat2_index.endsWith('.tar.gz')) { - ch_hisat2_index = UNTAR_HISAT2_INDEX ( params.hisat2_index ).untar - } else { - ch_hisat2_index = file(params.hisat2_index) - } - } else { - ch_hisat2_index = HISAT2_BUILD ( ch_fasta, ch_gtf, ch_splicesites ).index - ch_hisat2_version = HISAT2_BUILD.out.version - } - } - - // - // Uncompress Salmon index or generate from scratch if required - // - ch_salmon_index = Channel.empty() - ch_salmon_version = Channel.empty() - if ('salmon' in prepare_tool_indices) { - if (params.salmon_index) { - if (params.salmon_index.endsWith('.tar.gz')) { - ch_salmon_index = UNTAR_SALMON_INDEX ( params.salmon_index ).untar - } else { - ch_salmon_index = file(params.salmon_index) - } - } else { - ch_salmon_index = SALMON_INDEX ( ch_fasta, ch_transcript_fasta ).index - ch_salmon_version = SALMON_INDEX.out.version - } - } - emit: fasta = ch_fasta // path: genome.fasta fai = ch_fasta_fai // path: genome.fasta.fai dict = ch_fasta_dict // path: genome.fasta.dict gtf = ch_gtf // path: genome.gtf gene_bed = ch_gene_bed // path: gene.bed - transcript_fasta = ch_transcript_fasta // path: transcript.fasta chrom_sizes = ch_chrom_sizes // path: genome.sizes - splicesites = ch_splicesites // path: genome.splicesites.txt star_index = ch_star_index // path: star/index/ - rsem_index = ch_rsem_index // path: rsem/index/ - hisat2_index = ch_hisat2_index // path: hisat2/index/ - salmon_index = ch_salmon_index // path: salmon/index/ star_version = ch_star_version // path: *.version.txt - rsem_version = ch_rsem_version // path: *.version.txt - hisat2_version = ch_hisat2_version // path: *.version.txt - salmon_version = ch_salmon_version // path: *.version.txt gffread_version = ch_gffread_version // path: *.version.txt } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index d8130a55..d82b01f9 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -26,6 +26,10 @@ for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} if (params.input) ch_input = file(params.input) else exit 1, 'Input samplesheet not specified!' +if(!params.star_index and !params.gtf){ + exit 1, "GTF file is required to build a STAR reference index! Use option -gtf to provide a GTF file." +} + /* ======================================================================================== CONFIG FILES @@ -91,8 +95,6 @@ if (params.save_align_intermeds) { modules['samtools_index_genome'].publish_files.put('csi','') } -def biotype = params.gencode ? "gene_type" : params.featurecounts_group_type - if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' @@ -109,7 +111,7 @@ if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: [:]) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) @@ -148,13 +150,14 @@ workflow RNAVAR { ch_software_versions = Channel.empty() - PREPARE_GENOME(prepareToolIndices, biotype) - ch_genome_gtf = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) + PREPARE_GENOME(prepareToolIndices) + ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) ch_software_versions = Channel.empty() ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) INPUT_CHECK(ch_input) + .reads .map{ meta, fastq -> meta.id = meta.id.split('_')[0..-2].join('_') [meta, fastq]} @@ -165,11 +168,16 @@ workflow RNAVAR { multiple: fastq.size() > 1 return [meta, fastq.flatten()]} .set{ch_fastq} + //ch_software_versions = ch_software_versions.mix(INPUT_CHECK.out.version.first().ifEmpty(null)) + + ch_fastq.multiple.view() // MODULE: Concatenate FastQ files from same sample if required CAT_FASTQ(ch_fastq.multiple) + .reads .mix(ch_fastq.single) .set{ch_cat_fastq} + //ch_software_versions = ch_software_versions.mix(CAT_FASTQ.out.version.first().ifEmpty(null)) // MODULE: Run FastQC FASTQC(ch_cat_fastq) @@ -178,7 +186,7 @@ workflow RNAVAR { // PREPARE THE INTERVAL LIST FROM GTF FILE ch_interval_list = Channel.empty() - GATK4_BEDTOINTERVALLIST(ch_genome_gtf, PREPARE_GENOME.out.dict) + GATK4_BEDTOINTERVALLIST(ch_genome_bed, PREPARE_GENOME.out.dict) ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) @@ -235,7 +243,7 @@ workflow RNAVAR { known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() - ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] } + ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() GATK4_BASERECALIBRATOR( bam_splitncigar, @@ -254,7 +262,8 @@ workflow RNAVAR { bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() - ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] } + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() + RECALIBRATE( ('bamqc' in params.skip_qc), ('samtools' in params.skip_qc), From c625b25bb98842d18b363af7346223e8e79cea86 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 11 Nov 2021 17:23:42 +0100 Subject: [PATCH 041/235] cleaned --- CHANGELOG.md | 16 - CITATIONS.md | 32 -- CODE_OF_CONDUCT.md | 111 ---- LICENSE | 21 - README.md | 89 --- assets/email_template.html | 53 -- assets/email_template.txt | 40 -- assets/multiqc_config.yaml | 11 - assets/nf-core-rnavar_logo.png | Bin 16469 -> 0 bytes assets/samplesheet_test.csv | 2 - assets/schema_input.json | 39 -- assets/sendmail_template.txt | 53 -- bin/check_samplesheet.py | 152 ----- bin/fasta2gtf.py | 77 --- bin/gtf2bed | 122 ----- bin/scrape_software_versions.py | 36 -- conf/base.config | 50 -- conf/igenomes.config | 422 -------------- conf/modules.config | 131 ----- conf/test.config | 40 -- conf/test_full.config | 21 - docs/README.md | 10 - docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/images/nf-core-rnavar_logo.png | Bin 32098 -> 0 bytes docs/output.md | 66 --- docs/usage.md | 282 ---------- lib/NfcoreSchema.groovy | 517 ------------------ lib/NfcoreTemplate.groovy | 270 --------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 94 ---- lib/WorkflowRnavar.groovy | 59 -- lib/WorkflowSraDownload.groovy | 21 - lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 67 --- modules.json | 83 --- modules/local/functions.nf | 68 --- modules/local/gatk4/applybqsr/functions.nf | 68 --- modules/local/gatk4/applybqsr/main.nf | 46 -- modules/local/gatk4/applybqsr/meta.yml | 58 -- .../local/gatk4/haplotypecaller/functions.nf | 68 --- modules/local/gatk4/haplotypecaller/main.nf | 60 -- modules/local/gatk4/haplotypecaller/meta.yml | 63 --- modules/local/gatk4/mergevcfs/functions.nf | 68 --- modules/local/gatk4/mergevcfs/main.nf | 49 -- modules/local/gatk4/mergevcfs/meta.yml | 42 -- .../local/gatk4/splitncigarreads/functions.nf | 68 --- modules/local/gatk4/splitncigarreads/main.nf | 43 -- modules/local/gatk4/splitncigarreads/meta.yml | 40 -- .../gatk4/variantfiltration/functions.nf | 68 --- modules/local/gatk4/variantfiltration/main.nf | 44 -- .../local/gatk4/variantfiltration/meta.yml | 47 -- modules/local/get_chrom_sizes.nf | 34 -- modules/local/get_software_versions.nf | 33 -- modules/local/gtf2bed.nf | 30 - modules/local/gtf_gene_filter.nf | 30 - modules/local/samplesheet_check.nf | 31 -- .../nf-core/modules/cat/fastq/functions.nf | 68 --- modules/nf-core/modules/cat/fastq/main.nf | 47 -- 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modules/nf-core/modules/gunzip/functions.nf | 68 --- modules/nf-core/modules/gunzip/main.nf | 35 -- modules/nf-core/modules/gunzip/meta.yml | 27 - .../nf-core/modules/hisat2/build/functions.nf | 68 --- modules/nf-core/modules/hisat2/build/main.nf | 70 --- modules/nf-core/modules/hisat2/build/meta.yml | 42 -- .../hisat2/extractsplicesites/functions.nf | 68 --- .../modules/hisat2/extractsplicesites/main.nf | 36 -- .../hisat2/extractsplicesites/meta.yml | 34 -- modules/nf-core/modules/multiqc/functions.nf | 68 --- modules/nf-core/modules/multiqc/main.nf | 35 -- modules/nf-core/modules/multiqc/meta.yml | 39 -- .../picard/markduplicates/functions.nf | 68 --- .../modules/picard/markduplicates/main.nf | 49 -- .../modules/picard/markduplicates/meta.yml | 46 -- .../modules/qualimap/bamqc/functions.nf | 68 --- .../nf-core/modules/qualimap/bamqc/main.nf | 61 --- .../nf-core/modules/qualimap/bamqc/meta.yml | 49 -- .../rsem/preparereference/functions.nf | 68 --- 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100644 workflows/rnavar.nf diff --git a/CHANGELOG.md b/CHANGELOG.md deleted file mode 100644 index cb967546..00000000 --- a/CHANGELOG.md +++ /dev/null @@ -1,16 +0,0 @@ -# nf-core/rnavar: Changelog - -The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) -and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). - -## v1.0dev - [date] - -Initial release of nf-core/rnavar, created with the [nf-core](https://nf-co.re/) template. - -### `Added` - -### `Fixed` - -### `Dependencies` - -### `Deprecated` diff --git a/CITATIONS.md b/CITATIONS.md deleted file mode 100644 index cb19b8e1..00000000 --- a/CITATIONS.md +++ /dev/null @@ -1,32 +0,0 @@ -# nf-core/rnavar: Citations - -## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) - -> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. - -## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) - -> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. - -## Pipeline tools - -* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - -* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. - -## Software packaging/containerisation tools - -* [Anaconda](https://anaconda.com) - > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. - -* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) - > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. - -* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) - > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. - -* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) - -* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) - > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md deleted file mode 100644 index f4fd052f..00000000 --- a/CODE_OF_CONDUCT.md +++ /dev/null @@ -1,111 +0,0 @@ -# Code of Conduct at nf-core (v1.0) - -## Our Pledge - -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - -- Age -- Body size -- Familial status -- Gender identity and expression -- Geographical location -- Level of experience -- Nationality and national origins -- Native language -- Physical and neurological ability -- Race or ethnicity -- Religion -- Sexual identity and orientation -- Socioeconomic status - -Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. - -## Preamble - -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. - -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. - -nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. - -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. - -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. - -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. - -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re - -## Our Responsibilities - -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. - -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. - -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. - -## When are where does this Code of Conduct apply? - -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: - -- Communicating with an official project email address. -- Communicating with community members within the nf-core Slack channel. -- Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. -- Representing nf-core on social media. This includes both official and personal accounts. - -## nf-core cares 😊 - -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): - -- Ask for consent before sharing another community member’s personal information (including photographs) on social media. -- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) -- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) -- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) -- Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. -- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) -- Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) - -## nf-core frowns on 😕 - -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. - -- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. -- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. -- Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. - -### Online Trolling - -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. - -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. - -## Procedures for Reporting CoC violations - -If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. - -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). - -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. - -All reports will be handled with utmost discretion and confidentially. - -## Attribution and Acknowledgements - -- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) -- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) -- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) -- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) - -## Changelog - -### v1.0 - March 12th, 2021 - -- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/LICENSE b/LICENSE deleted file mode 100644 index bc2712ac..00000000 --- a/LICENSE +++ /dev/null @@ -1,21 +0,0 @@ -MIT License - -Copyright (c) Praveen Raj - -Permission is hereby granted, free of charge, to any person obtaining a copy -of this software and associated documentation files (the "Software"), to deal -in the Software without restriction, including without limitation the rights -to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -copies of the Software, and to permit persons to whom the Software is -furnished to do so, subject to the following conditions: - -The above copyright notice and this permission notice shall be included in all -copies or substantial portions of the Software. - -THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE -SOFTWARE. diff --git a/README.md b/README.md deleted file mode 100644 index cd1ee1c7..00000000 --- a/README.md +++ /dev/null @@ -1,89 +0,0 @@ -# ![nf-core/rnavar](docs/images/nf-core-rnavar_logo.png) - -[![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) -[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) -[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) - -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) -[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) -[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) -[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) - -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnavar-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnavar) -[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core) -[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) - -## Introduction - -**nf-core/rnavar** is a bioinformatics best-practice pipeline for RNA-Seq variant analysis. - -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! - - - - -## Pipeline summary - - - -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) - -## Quick Start - -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`) - -2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ - -3. Download the pipeline and test it on a minimal dataset with a single command: - - ```console - nextflow run nf-core/rnavar -profile test, - ``` - - > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs. - > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. - -4. Start running your own analysis! - - - - ```console - nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh37 - ``` - -## Documentation - -The nf-core/rnavar pipeline comes with documentation about the pipeline [usage](https://nf-co.re/rnavar/usage), [parameters](https://nf-co.re/rnavar/parameters) and [output](https://nf-co.re/rnavar/output). - -## Credits - -nf-core/rnavar was originally written by [Praveen Raj](https://github.com/praveenraj2018) and [Maxime Garcia](https://github.com/maxulysse). - -We thank the following people for their extensive assistance in the development of this pipeline: - - - -## Contributions and Support - -If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). - -For further information or help, don't hesitate to get in touch on the [Slack `#rnavar` channel](https://nfcore.slack.com/channels/rnavar) (you can join with [this invite](https://nf-co.re/join/slack)). - -## Citations - - - - - -An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. - -You can cite the `nf-core` publication as follows: - -> **The nf-core framework for community-curated bioinformatics pipelines.** -> -> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. -> -> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). diff --git a/assets/email_template.html b/assets/email_template.html deleted file mode 100644 index 1b6ecc5f..00000000 --- a/assets/email_template.html +++ /dev/null @@ -1,53 +0,0 @@ - - - - - - - - nf-core/rnavar Pipeline Report - - -
- - - -

nf-core/rnavar v${version}

-

Run Name: $runName

- -<% if (!success){ - out << """ -
-

nf-core/rnavar execution completed unsuccessfully!

-

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

-

The full error message was:

- 
${errorReport}
-
- """ -} else { - out << """ -
- nf-core/rnavar execution completed successfully! -
- """ -} -%> - -

The workflow was completed at $dateComplete (duration: $duration)

-

The command used to launch the workflow was as follows:

-
$commandLine
- -

Pipeline Configuration:

- - - <% out << summary.collect{ k,v -> "" }.join("\n") %> - -
$k
$v
- -

nf-core/rnavar

-

https://github.com/nf-core/rnavar

- -
- - - diff --git a/assets/email_template.txt b/assets/email_template.txt deleted file mode 100644 index 2c43ec61..00000000 --- a/assets/email_template.txt +++ /dev/null @@ -1,40 +0,0 @@ ----------------------------------------------------- - ,--./,-. - ___ __ __ __ ___ /,-._.--~\\ - |\\ | |__ __ / ` / \\ |__) |__ } { - | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, - `._,._,' - nf-core/rnavar v${version} ----------------------------------------------------- - -Run Name: $runName - -<% if (success){ - out << "## nf-core/rnavar execution completed successfully! ##" -} else { - out << """#################################################### -## nf-core/rnavar execution completed unsuccessfully! ## -#################################################### -The exit status of the task that caused the workflow execution to fail was: $exitStatus. -The full error message was: - -${errorReport} -""" -} %> - - -The workflow was completed at $dateComplete (duration: $duration) - -The command used to launch the workflow was as follows: - - $commandLine - - - -Pipeline Configuration: ------------------------ -<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> - --- -nf-core/rnavar -https://github.com/nf-core/rnavar diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml deleted file mode 100644 index bd717cf9..00000000 --- a/assets/multiqc_config.yaml +++ /dev/null @@ -1,11 +0,0 @@ -report_comment: > - This report has been generated by the nf-core/rnavar - analysis pipeline. For information about how to interpret these results, please see the - documentation. -report_section_order: - software_versions: - order: -1000 - nf-core-rnavar-summary: - order: -1001 - -export_plots: true diff --git a/assets/nf-core-rnavar_logo.png b/assets/nf-core-rnavar_logo.png deleted file mode 100644 index 482af6ee764c26783cee9e3e82ab3a7c5a892415..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 16469 zcmX|J1yogAn7))C-5`y0hk%H5N=b-xi*%PXNDC55cXvy7w;9JmfeA>BX#~yxPM0cvu^l8-j4~9WnmGq7+vP;}>_IIHMB!NhTePo|Syj zz4+^4+>qwhklR?eXZbSZB?3eg3F4!STw1@CXi-{!nft98vTDmqf-Z&ZTTNZj!9A_* zFvLAAm#Dc%O@bcOx%y7nmQ|?kzl*mLCC&sfp})NL*Jl%5O6~dY>Ix*)B; zAh&p9C;;4nZdl1W-1EXAf^>2D^ldV}OnCFZ*S0_%^XKKu-pdV#gbrdAy zF8d1J_q~34wBgfH<8jaVc6vdPn2`~#+r?f`)pyjL3%>g*V>Cz2l1>h*`PyE@vsQ6s z8JXawOVWA=EmFw8Z}c3=J3T$Vb@(G%G^)Q6N$GMJqxK_yL6Mz)1G)JAPc6hJI2dW+ z>X?`RbV5a3Qi8j%+rRV1W&D5N6ym2FK}X(199g=F@fnyOz46GM&~&Lvqog~fq-^KwBfE=X!%N%#yg8eBE3@e0Q%~=gBIcI 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-Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" - -${mqcFileObj. - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' )} -""" -}} -%> - ---nfcoremimeboundary-- diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 209540be..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,152 +0,0 @@ -#!/usr/bin/env python - -import os -import sys -import errno -import argparse - - -def parse_args(args=None): - Description = "Reformat nf-core/rnavar samplesheet file and check its contents." - Epilog = "Example usage: python check_samplesheet.py " - - parser = argparse.ArgumentParser(description=Description, epilog=Epilog) - parser.add_argument("FILE_IN", help="Input samplesheet file.") - parser.add_argument("FILE_OUT", help="Output file.") - return parser.parse_args(args) - -def make_dir(path): - if len(path) > 0: - try: - os.makedirs(path) - except OSError as exception: - if exception.errno != errno.EEXIST: - raise exception - - -def print_error(error, context="Line", context_str=""): - error_str = "ERROR: Please check samplesheet -> {}".format(error) - if context != "" and context_str != "": - error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( - error, context.strip(), context_str.strip() - ) - print(error_str) - sys.exit(1) - - -# TODO nf-core: Update the check_samplesheet function -def check_samplesheet(file_in, file_out): - """ - This function checks that the samplesheet follows the following structure: - - sample,fastq_1,fastq_2,strandedness - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,,forward - - For an example see: - https://github.com/nf-core/test-datasets/blob/rnaseq/samplesheet/v3.1/samplesheet_test.csv - """ - - sample_mapping_dict = {} - with open(file_in, "r") as fin: - - ## Check header - MIN_COLS = 3 - HEADER = ["sample", "fastq_1", "fastq_2", "strandedness"] - header = [x.strip('"') for x in fin.readline().strip().split(",")] - if header[: len(HEADER)] != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) - sys.exit(1) - - ## Check sample entries - for line in fin: - lspl = [x.strip().strip('"') for x in line.strip().split(",")] - - # Check valid number of columns per row - if len(lspl) < len(HEADER): - print_error( - "Invalid number of columns (minimum = {})!".format(len(HEADER)), - "Line", - line, - ) - num_cols = len([x for x in lspl if x]) - if num_cols < MIN_COLS: - print_error( - "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), - "Line", - line, - ) - - ## Check sample name entries - sample, fastq_1, fastq_2, strandedness = lspl[:len(HEADER)] - sample = sample.replace(" ", "_") - if not sample: - print_error("Sample entry has not been specified!", "Line", line) - - ## Check FastQ file extension - for fastq in [fastq_1, fastq_2]: - if fastq: - if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line", line) - if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): - print_error( - "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line", - line, - ) - - ## Check strandedness - strandednesses = ['unstranded', 'forward', 'reverse'] - if strandedness: - if strandedness not in strandednesses: - print_error(f"Strandedness must be one of '{', '.join(strandednesses)}'!", 'Line', line) - else: - print_error(f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.", 'Line', line) - - ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2] - if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ['0', fastq_1, fastq_2, strandedness] - elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ['1', fastq_1, fastq_2, strandedness] - else: - print_error("Invalid combination of columns provided!", "Line", line) - - ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, strandedness ]]} - if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = [sample_info] - else: - if sample_info in sample_mapping_dict[sample]: - print_error("Samplesheet contains duplicate rows!", "Line", line) - else: - sample_mapping_dict[sample].append(sample_info) - - ## Write validated samplesheet with appropriate columns - if len(sample_mapping_dict) > 0: - out_dir = os.path.dirname(file_out) - make_dir(out_dir) - with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2", "strandedness"]) + "\n") - for sample in sorted(sample_mapping_dict.keys()): - - ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) - - ## Check that multiple runs of the same sample are of the same strandness - if not all(x[-1] == sample_mapping_dict[sample][0][-1] for x in sample_mapping_dict[sample]): - print_error("Multiple runs of a sample must be of the same strandness!", "Sample: {}".format(sample)) - - for idx, val in enumerate(sample_mapping_dict[sample]): - fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") - else: - print_error("No entries to process!", "Samplesheet: {}".format(file_in)) - - -def main(args=None): - args = parse_args(args) - check_samplesheet(args.FILE_IN, args.FILE_OUT) - -if __name__ == "__main__": - sys.exit(main()) diff --git a/bin/fasta2gtf.py b/bin/fasta2gtf.py deleted file mode 100755 index 197ba12c..00000000 --- a/bin/fasta2gtf.py +++ /dev/null @@ -1,77 +0,0 @@ -#!/usr/bin/env python -""" -Read a custom fasta file and create a custom GTF containing each entry -""" -import argparse -from itertools import groupby -import logging - -# Create a logger -logging.basicConfig(format='%(name)s - %(asctime)s %(levelname)s: %(message)s') -logger = logging.getLogger(__file__) -logger.setLevel(logging.INFO) - - -def fasta_iter(fasta_name): - """ - modified from Brent Pedersen - Correct Way To Parse A Fasta File In Python - given a fasta file. yield tuples of header, sequence - - Fasta iterator from https://www.biostars.org/p/710/#120760 - """ - with open(fasta_name) as fh: - # ditch the boolean (x[0]) and just keep the header or sequence since - # we know they alternate. - faiter = (x[1] for x in groupby(fh, lambda line: line[0] == ">")) - for header in faiter: - # drop the ">" - headerStr = header.__next__()[1:].strip() - - # join all sequence lines to one. - seq = "".join(s.strip() for s in faiter.__next__()) - - yield (headerStr, seq) - - -def fasta2gtf(fasta, output, biotype): - fiter = fasta_iter(fasta) - # GTF output lines - lines = [] - attributes = \ - 'exon_id "{name}.1"; exon_number "1";{biotype} gene_id "{name}_gene"; gene_name "{name}_gene"; gene_source "custom"; transcript_id "{name}_gene"; transcript_name "{name}_gene";\n' - line_template = \ - "{name}\ttransgene\texon\t1\t{length}\t.\t+\t.\t" + attributes - - for ff in fiter: - name, seq = ff - # Use first ID as separated by spaces as the "sequence name" - # (equivalent to "chromosome" in other cases) - seqname = name.split()[0] - # Remove all spaces - name = seqname.replace(' ', '_') - length = len(seq) - biotype_attr = '' - if biotype: - biotype_attr = f' {biotype} "transgene";' - line = line_template.format( - name=name, length=length, biotype=biotype_attr) - lines.append(line) - - with open(output, 'w') as f: - f.write(''.join(lines)) - - -if __name__ == "__main__": - parser = argparse.ArgumentParser( - description="""Convert a custom fasta (e.g. transgene) - to a GTF annotation.""") - parser.add_argument("fasta", type=str, help="Custom transgene sequence") - parser.add_argument( - "-o", "--output", dest='output', - default='transgenes.gtf', type=str, help="Gene annotation GTF output") - parser.add_argument( - "-b", "--biotype", dest='biotype', - default='', type=str, help="Name of gene biotype attribute to use in last column of GTF entry") - args = parser.parse_args() - fasta2gtf(args.fasta, args.output, args.biotype) diff --git a/bin/gtf2bed b/bin/gtf2bed deleted file mode 100755 index 4da5c046..00000000 --- a/bin/gtf2bed +++ /dev/null @@ -1,122 +0,0 @@ -#!/usr/bin/env perl - -# Copyright (c) 2011 Erik Aronesty (erik@q32.com) -# -# Permission is hereby granted, free of charge, to any person obtaining a copy -# of this software and associated documentation files (the "Software"), to deal -# in the Software without restriction, including without limitation the rights -# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -# copies of the Software, and to permit persons to whom the Software is -# furnished to do so, subject to the following conditions: -# -# The above copyright notice and this permission notice shall be included in -# all copies or substantial portions of the Software. -# -# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN -# THE SOFTWARE. -# -# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT. - -use Getopt::Long; - -my $extended; -GetOptions("x"=>\$extended); - -$in = shift @ARGV; - -my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n"; -open IN, $in_cmd; - -while () { - $gff = 2 if /^##gff-version 2/; - $gff = 3 if /^##gff-version 3/; - next if /^#/ && $gff; - - s/\s+$//; - # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr - my @f = split /\t/; - if ($gff) { - # most ver 2's stick gene names in the id field - ($id) = $f[8]=~ /\bID="([^"]+)"/; - # most ver 3's stick unquoted names in the name field - ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3; - } else { - ($id) = $f[8]=~ /transcript_id "([^"]+)"/; - } - - next unless $id && $f[0]; - - if ($f[2] eq 'exon') { - die "no position at exon on line $." if ! $f[3]; - # gff3 puts :\d in exons sometimes - $id =~ s/:\d+$// if $gff == 3; - push @{$exons{$id}}, \@f; - # save lowest start - $trans{$id} = \@f if !$trans{$id}; - } elsif ($f[2] eq 'start_codon') { - #optional, output codon start/stop as "thick" region in bed - $sc{$id}->[0] = $f[3]; - } elsif ($f[2] eq 'stop_codon') { - $sc{$id}->[1] = $f[4]; - } elsif ($f[2] eq 'miRNA' ) { - $trans{$id} = \@f if !$trans{$id}; - push @{$exons{$id}}, \@f; - } -} - -for $id ( - # sort by chr then pos - sort { - $trans{$a}->[0] eq $trans{$b}->[0] ? - $trans{$a}->[3] <=> $trans{$b}->[3] : - $trans{$a}->[0] cmp $trans{$b}->[0] - } (keys(%trans)) ) { - my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}}; - my ($cds, $cde); - ($cds, $cde) = @{$sc{$id}} if $sc{$id}; - - # sort by pos - my @ex = sort { - $a->[3] <=> $b->[3] - } @{$exons{$id}}; - - my $beg = $ex[0][3]; - my $end = $ex[-1][4]; - - if ($dir eq '-') { - # swap - $tmp=$cds; - $cds=$cde; - $cde=$tmp; - $cds -= 2 if $cds; - $cde += 2 if $cde; - } - - # not specified, just use exons - $cds = $beg if !$cds; - $cde = $end if !$cde; - - # adjust start for bed - --$beg; --$cds; - - my $exn = @ex; # exon count - my $exst = join ",", map {$_->[3]-$beg-1} @ex; # exon start - my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex; # exon size - - my $gene_id; - my $extend = ""; - if ($extended) { - ($gene_id) = $attr =~ /gene_name "([^"]+)"/; - ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id; - $extend="\t$gene_id"; - } - # added an extra comma to make it look exactly like ucsc's beds - print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n"; -} - -close IN; diff --git a/bin/scrape_software_versions.py b/bin/scrape_software_versions.py deleted file mode 100755 index 77096990..00000000 --- a/bin/scrape_software_versions.py +++ /dev/null @@ -1,36 +0,0 @@ -#!/usr/bin/env python -from __future__ import print_function -import os - -results = {} -version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")] -for version_file in version_files: - - software = version_file.replace(".version.txt", "") - if software == "pipeline": - software = "nf-core/rnavar" - - with open(version_file) as fin: - version = fin.read().strip() - results[software] = version - -# Dump to YAML -print( - """ -id: 'software_versions' -section_name: 'nf-core/rnavar Software Versions' -section_href: 'https://github.com/nf-core/rnavar' -plot_type: 'html' -description: 'are collected at run time from the software output.' -data: | -
-""" -) -for k, v in sorted(results.items()): - print("
{}
{}
".format(k, v)) -print("
") - -# Write out as tsv file: -with open("software_versions.tsv", "w") as f: - for k, v in sorted(results.items()): - f.write("{}\t{}\n".format(k, v)) diff --git a/conf/base.config b/conf/base.config deleted file mode 100644 index e3d4076a..00000000 --- a/conf/base.config +++ /dev/null @@ -1,50 +0,0 @@ -/* -======================================================================================== - nf-core/rnavar Nextflow base config file -======================================================================================== - A 'blank slate' config file, appropriate for general use on most high performance - compute environments. Assumes that all software is installed and available on - the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. ----------------------------------------------------------------------------------------- -*/ - -process { - - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } - - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' - - // Process-specific resource requirements - withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } - } - withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - } - withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } - } - withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } - } - withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } - } - withLabel:error_ignore { - errorStrategy = 'ignore' - } - withLabel:error_retry { - errorStrategy = 'retry' - maxRetries = 2 - } -} diff --git a/conf/igenomes.config b/conf/igenomes.config deleted file mode 100644 index a270df7f..00000000 --- a/conf/igenomes.config +++ /dev/null @@ -1,422 +0,0 @@ -/* -======================================================================================== - Nextflow config file for iGenomes paths -======================================================================================== - Defines reference genomes using iGenome paths. - Can be used by any config that customises the base path using: - $params.igenomes_base / --igenomes_base ----------------------------------------------------------------------------------------- -*/ - -params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" - } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} diff --git a/conf/modules.config b/conf/modules.config deleted file mode 100644 index 865c1b44..00000000 --- a/conf/modules.config +++ /dev/null @@ -1,131 +0,0 @@ -/* -======================================================================================== - Config file for defining DSL2 per module options -======================================================================================== - Available keys to override module options: - args = Additional arguments appended to command in module. - args2 = Second set of arguments appended to command in module (multi-tool modules). - args3 = Third set of arguments appended to command in module (multi-tool modules). - publish_dir = Directory to publish results. - publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path - If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path - If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" - is appended as a directory to "publish_dir" path - If publish_by_meta = false / null - No directories are appended to "publish_dir" path - publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension - The value of "directory" is appended to the standard "publish_dir" path as defined above. - If publish_files = null (unspecified) - All files are published. - If publish_files = false - No files are published. - suffix = File name suffix for output files. ----------------------------------------------------------------------------------------- -*/ - -params { - modules { - 'cat_fastq' { - publish_dir = 'fastq' - } - 'star_genomegenerate' { - args = "--sjdbOverhang ${params.read_length - 1}" - publish_dir = 'genome/index' - } - 'star_align' { - args = "--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" - publish_files = false - } - 'samtools_sort_genome' { - suffix = '.sorted' - publish_files = false - } - 'samtools_index_genome' { - args = '' - suffix = '.sorted' - publish_files = false - } - 'picard_markduplicates' { - args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' - suffix = '.markdup.sorted' - publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'picard_markduplicates_samtools' { - args = '' - suffix = '.markdup.sorted' - publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'gatk_splitncigar_options' { - args = '' - suffix = '.split_cigar' - publish_files = false - } - 'gatk_baserecalibrator' { - args = '' - suffix = '' - publish_files = false - } - 'gatk_applybqsr' { - args = '' - suffix = '.markdup.recalibrated' - publish_files = false - } - 'gatk_haplotypecaller' { - args = '-dont-use-soft-clipped-bases --standard-min-confidence-threshold-for-calling 20' - suffix = '' - publish_files = false - } - 'gatk_intervallisttools' { - args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' - suffix = '' - publish_files = false - } - 'gatk_bedtointervallist' { - args = '' - publish_files = false - } - // RECALIBRATE - 'applybqsr' { - suffix = '.recal' - publish_files = false - } - 'merge_bam_recalibrate' { - suffix = '.recal.merge' - publish_by_meta = true - publish_files = ['bam':'', 'bai':''] - publish_dir = 'preprocessing' - } - 'samtools_index_recalibrate' { - suffix = 'recalibrated' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bam':'', 'bai':''] - } - 'samtools_stats_recalibrate' { - publish_by_meta = true - publish_dir = 'reports/samtools_stats' - } - 'gatk_mergevcfs' { - suffix = '.haplotypecaller' - publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - 'gatk_variantfilter' { - args = '' - suffix = '.filtered' - publish_by_meta = true - publish_dir = 'variant_filtering' - publish_files = ['vcf':'', 'vcf.idx':''] - } - 'fastqc' { - args = "--quiet" - publish_files = false - } - 'multiqc' { - args = "" - publish_dir = 'reports' - } - } -} diff --git a/conf/test.config b/conf/test.config deleted file mode 100644 index 95148324..00000000 --- a/conf/test.config +++ /dev/null @@ -1,40 +0,0 @@ -/* -======================================================================================== - Nextflow config file for running minimal tests -======================================================================================== - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/rnavar -profile test, - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = 6.GB - max_time = 6.h - - // Input data - input = 'assets/samplesheet_test.csv' - - // Genome references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' - fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' - fasta_dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' - gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' - //gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' - - // Known genome resources (optional) - dbsnp_vcf = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' - dbsnp_vcf_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' - known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' - known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' - - // STAR index (optional) - //star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' -} diff --git a/conf/test_full.config b/conf/test_full.config deleted file mode 100644 index bf10beb0..00000000 --- a/conf/test_full.config +++ /dev/null @@ -1,21 +0,0 @@ -/* -======================================================================================== - Nextflow config file for running full-size tests -======================================================================================== - Defines input files and everything required to run a full size pipeline test. - - Use as follows: - nextflow run nf-core/rnavar -profile test_full, - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' - - // Parameters for full-size test - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_full.csv' - genome = 'GRCh37' - pseudo_aligner = 'salmon' -} diff --git a/docs/README.md b/docs/README.md deleted file mode 100644 index e5f538d3..00000000 --- a/docs/README.md +++ /dev/null @@ -1,10 +0,0 @@ -# nf-core/rnavar: Documentation - -The nf-core/rnavar documentation is split into the following pages: - -* [Usage](usage.md) - * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. -* [Output](output.md) - * An overview of the different results produced by the pipeline and how to interpret them. - -You can find a lot more documentation about installing, 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Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. - -The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - -## Pipeline overview - -The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - -* [FastQC](#fastqc) - Raw read QC -* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - -### FastQC - -
-Output files - -* `fastqc/` - * `*_fastqc.html`: FastQC report containing quality metrics. - * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. - -
- -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) - -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) - -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) - -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. - -### MultiQC - -
-Output files - -* `multiqc/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. - -
- -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. - -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . - -### Pipeline information - -
-Output files - -* `pipeline_info/` - * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`. - * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. - -
- -[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md deleted file mode 100644 index 585a7d7f..00000000 --- a/docs/usage.md +++ /dev/null @@ -1,282 +0,0 @@ -# nf-core/rnavar: Usage - -## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnavar/usage](https://nf-co.re/rnavar/usage) - -> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ - -## Introduction - -## Samplesheet input - -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. - -```console ---input '[path to samplesheet file]' -``` - -### Multiple runs of the same sample - -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: - -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` - -### Full samplesheet - -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. - -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. - -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, -``` - -| Column | Description | -|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | - -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. - -## Running the pipeline - -The typical command for running the pipeline is as follows: - -```console -nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh37 -profile docker -``` - -This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. - -Note that the pipeline will create the following files in your working directory: - -```console -work # Directory containing the nextflow working files -results # Finished results (configurable, see below) -.nextflow_log # Log file from Nextflow -# Other nextflow hidden files, eg. history of pipeline runs and old logs. -``` - -### Updating the pipeline - -When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: - -```console -nextflow pull nf-core/rnavar -``` - -### Reproducibility - -It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. - -First, go to the [nf-core/rnavar releases page](https://github.com/nf-core/rnavar/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. - -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. - -## Core Nextflow arguments - -> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). - -### `-profile` - -Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. - -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/). - -> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. - -The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). - -Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! -They are loaded in sequence, so later profiles can overwrite earlier profiles. - -If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. - -* `docker` - * A generic configuration profile to be used with [Docker](https://docker.com/) -* `singularity` - * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) -* `podman` - * A generic configuration profile to be used with [Podman](https://podman.io/) -* `shifter` - * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) -* `charliecloud` - * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) -* `conda` - * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. -* `test` - * A profile with a complete configuration for automated testing - * Includes links to test data so needs no other parameters - -### `-resume` - -Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. - -You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. - -### `-c` - -Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. - -## Custom configuration - -### Resource requests - -Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. - -For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: - -```console -[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' - -Caused by: - Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) - -Command executed: - STAR \ - --genomeDir star \ - --readFilesIn WT_REP1_trimmed.fq.gz \ - --runThreadN 2 \ - --outFileNamePrefix WT_REP1. \ - - -Command exit status: - 137 - -Command output: - (empty) - -Command error: - .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. -Work dir: - /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb - -Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` -``` - -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. - -```nextflow -process { - withName: STAR_ALIGN { - memory = 100.GB - } -} -``` - -> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. - -### Tool-specific options - -For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes. - -The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: - -* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. - -* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. - -The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. - -When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. - -As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. - -Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. - -As you will see in the example below, we have: - -* appended `--outFilterMismatchNmax 16` to the default `args` used by the module. -* changed the default `publish_dir` value to where the files will eventually be published in the main results directory. -* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`. - -```nextflow -params { - modules { - 'star_align' { - args = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16" - publish_dir = "my_star_directory" - publish_files = ['out':'log', 'tab':'log', 'bam':''] - } - } -} -``` - -### Updating containers - -The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. - -1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) -2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) -3. Create the custom config accordingly: - - * For Docker: - - ```nextflow - process { - withName: PANGOLIN { - container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` - - * For Singularity: - - ```nextflow - process { - withName: PANGOLIN { - container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` - - * For Conda: - - ```nextflow - process { - withName: PANGOLIN { - conda = 'bioconda::pangolin=3.0.5' - } - } - ``` - -> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. - -### nf-core/configs - -In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. - -See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. - -If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). - -## Running in the background - -Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. - -The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. - -Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. -Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). - -## Nextflow memory requirements - -In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. -We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): - -```console -NXF_OPTS='-Xms1g -Xmx4g' -``` diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy deleted file mode 100755 index 8d6920dd..00000000 --- a/lib/NfcoreSchema.groovy +++ /dev/null @@ -1,517 +0,0 @@ -// -// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. -// - -import org.everit.json.schema.Schema -import org.everit.json.schema.loader.SchemaLoader -import org.everit.json.schema.ValidationException -import org.json.JSONObject -import org.json.JSONTokener -import org.json.JSONArray -import groovy.json.JsonSlurper -import groovy.json.JsonBuilder - -class NfcoreSchema { - - // - // Resolve Schema path relative to main workflow directory - // - public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { - return "${workflow.projectDir}/${schema_filename}" - } - - // - // Function to loop over all parameters defined in schema and check - // whether the given parameters adhere to the specifications - // - /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { - def has_error = false - //=====================================================================// - // Check for nextflow core params and unexpected params - def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text - def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') - def nf_params = [ - // Options for base `nextflow` command - 'bg', - 'c', - 'C', - 'config', - 'd', - 'D', - 'dockerize', - 'h', - 'log', - 'q', - 'quiet', - 'syslog', - 'v', - 'version', - - // Options for `nextflow run` command - 'ansi', - 'ansi-log', - 'bg', - 'bucket-dir', - 'c', - 'cache', - 'config', - 'dsl2', - 'dump-channels', - 'dump-hashes', - 'E', - 'entry', - 'latest', - 'lib', - 'main-script', - 'N', - 'name', - 'offline', - 'params-file', - 'pi', - 'plugins', - 'poll-interval', - 'pool-size', - 'profile', - 'ps', - 'qs', - 'queue-size', - 'r', - 'resume', - 'revision', - 'stdin', - 'stub', - 'stub-run', - 'test', - 'w', - 'with-charliecloud', - 'with-conda', - 'with-dag', - 'with-docker', - 'with-mpi', - 'with-notification', - 'with-podman', - 'with-report', - 'with-singularity', - 'with-timeline', - 'with-tower', - 'with-trace', - 'with-weblog', - 'without-docker', - 'without-podman', - 'work-dir' - ] - def unexpectedParams = [] - - // Collect expected parameters from the schema - def expectedParams = [] - for (group in schemaParams) { - for (p in group.value['properties']) { - expectedParams.push(p.key) - } - } - - for (specifiedParam in params.keySet()) { - // nextflow params - if (nf_params.contains(specifiedParam)) { - log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" - has_error = true - } - // unexpected params - def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } - def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { - // Temporarily remove camelCase/camel-case params #1035 - def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} - if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ - unexpectedParams.push(specifiedParam) - } - } - } - - //=====================================================================// - // Validate parameters against the schema - InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() - JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) - - // Remove anything that's in params.schema_ignore_params - raw_schema = removeIgnoredParams(raw_schema, params) - - Schema schema = SchemaLoader.load(raw_schema) - - // Clean the parameters - def cleanedParams = cleanParameters(params) - - // Convert to JSONObject - def jsonParams = new JsonBuilder(cleanedParams) - JSONObject params_json = new JSONObject(jsonParams.toString()) - - // Validate - try { - schema.validate(params_json) - } catch (ValidationException e) { - println '' - log.error 'ERROR: Validation of pipeline parameters failed!' - JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log) - println '' - has_error = true - } - - // Check for unexpected parameters - if (unexpectedParams.size() > 0) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - println '' - def warn_msg = 'Found unexpected parameters:' - for (unexpectedParam in unexpectedParams) { - warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" - } - log.warn warn_msg - log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" - println '' - } - - if (has_error) { - System.exit(1) - } - } - - // - // Beautify parameters for --help - // - public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - Integer num_hidden = 0 - String output = '' - output += 'Typical pipeline command:\n\n' - output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - Integer max_chars = paramsMaxChars(params_map) + 1 - Integer desc_indent = max_chars + 14 - Integer dec_linewidth = 160 - desc_indent - for (group in params_map.keySet()) { - Integer num_params = 0 - String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (group_params.get(param).hidden && !params.show_hidden_params) { - num_hidden += 1 - continue; - } - def type = '[' + group_params.get(param).type + ']' - def description = group_params.get(param).description - def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : '' - def description_default = description + colors.dim + defaultValue + colors.reset - // Wrap long description texts - // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap - if (description_default.length() > dec_linewidth){ - List olines = [] - String oline = "" // " " * indent - description_default.split(" ").each() { wrd -> - if ((oline.size() + wrd.size()) <= dec_linewidth) { - oline += wrd + " " - } else { - olines += oline - oline = wrd + " " - } - } - olines += oline - description_default = olines.join("\n" + " " * desc_indent) - } - group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' - num_params += 1 - } - group_output += '\n' - if (num_params > 0){ - output += group_output - } - } - if (num_hidden > 0){ - output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset - } - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Groovy Map summarising parameters/workflow options used by the pipeline - // - public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = workflow.containerEngine - } - if (workflow.container) { - workflow_summary['container'] = workflow.container - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def blacklist = ['hostnames'] - def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param) && !blacklist.contains(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value != null) { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir', '') - sub_string = sub_string.replace('\${projectDir}', '') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir', '') - sub_string = sub_string.replace('\${params.outdir}', '') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - // We have a default in the schema, and this isn't it - if (schema_value != null && params_value != schema_value) { - sub_params.put(param, params_value) - } - // No default in the schema, and this isn't empty - else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { - sub_params.put(param, params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - // - // Beautify parameters for summary and return as string - // - public static String paramsSummaryLog(workflow, params) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - String output = '' - def params_map = paramsSummaryMap(workflow, params) - def max_chars = paramsMaxChars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += colors.bold + group + colors.reset + '\n' - for (param in group_params.keySet()) { - output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' - } - output += '\n' - } - } - output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Loop over nested exceptions and print the causingException - // - private static void printExceptions(ex_json, params_json, log) { - def causingExceptions = ex_json['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (ex_json['pointerToViolation'] == '#') { - log.error "* ${ex_json['message']}" - } - // Error with specific param - else { - def param = ex_json['pointerToViolation'] - ~/^#\// - def param_val = params_json[param].toString() - log.error "* --${param}: ${ex_json['message']} (${param_val})" - } - } - for (ex in causingExceptions) { - printExceptions(ex, params_json, log) - } - } - - // - // Remove an element from a JSONArray - // - private static JSONArray removeElement(json_array, element) { - def list = [] - int len = json_array.length() - for (int i=0;i - if(raw_schema.keySet().contains('definitions')){ - raw_schema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { - raw_schema.get("properties").remove(ignore_param) - } - if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { - def cleaned_required = removeElement(raw_schema.required, ignore_param) - raw_schema.put("required", cleaned_required) - } - } - return raw_schema - } - - // - // Clean and check parameters relative to Nextflow native classes - // - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - // - // This function tries to read a JSON params file - // - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - // - // Method to actually read in JSON file using Groovy. - // Group (as Key), values are all parameters - // - Parameter1 as Key, Description as Value - // - Parameter2 as Key, Description as Value - // .... - // Group - // - - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - // - // Get maximum number of characters across all parameter names - // - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } -} diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index 44551e0a..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,270 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Check params.hostnames - // - public static void hostName(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (params.hostnames) { - try { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.info "=${colors.yellow}====================================================${colors.reset}=\n" + - "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + - " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + - "=${colors.yellow}====================================================${colors.reset}=" - } - } - } - } catch (Exception e) { - log.warn "[$workflow.manifest.name] Could not determine 'hostname' - skipping check. Reason: ${e.message}." - } - } - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - hostName(workflow, params, log) - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100755 index 18173e98..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - def required_channels = ['conda-forge', 'bioconda', 'defaults'] - def conda_check_failed = !required_channels.every { ch -> ch in channels } - - // Check that they are in the right order - conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) - conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) - - if (conda_check_failed) { - log.warn "=============================================================================\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + - " NB: The order of the channels matters!\n" + - "===================================================================================" - } - } - - // - // Join module args with appropriate spacing - // - public static String joinModuleArgs(args_list) { - return ' ' + args_list.join(' ') - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index f4e3f0a8..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,94 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/rnavar pipeline -// - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - // - // Print help to screen if required - // - public static String help(workflow, params, log) { - def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - def help_string = '' - help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) - help_string += NfcoreSchema.paramsHelp(workflow, params, command) - help_string += '\n' + citation(workflow) + '\n' - help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) - return help_string - } - - // - // Print parameter summary log to screen - // - public static String paramsSummaryLog(workflow, params, log) { - def summary_log = '' - summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) - summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) - summary_log += '\n' + citation(workflow) + '\n' - summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) - return summary_log - } - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log) { - // Print help to screen if required - if (params.help) { - log.info help(workflow, params, log) - System.exit(0) - } - - // Validate workflow parameters via the JSON schema - if (params.validate_params) { - NfcoreSchema.validateParameters(workflow, params, log) - } - - // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params, log) - - // Check that conda channels are set-up correctly - if (params.enable_conda) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check the hostnames against configured profiles - NfcoreTemplate.hostName(workflow, params, log) - - // Check input has been provided - if (!params.input) { - log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" - System.exit(1) - } - } - - // - // Get attribute from genome config file e.g. fasta - // - public static String getGenomeAttribute(params, attribute) { - def val = '' - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - val = params.genomes[ params.genome ][ attribute ] - } - } - return val - } -} diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy deleted file mode 100755 index 15dd1328..00000000 --- a/lib/WorkflowRnavar.groovy +++ /dev/null @@ -1,59 +0,0 @@ -// -// This file holds several functions specific to the workflow/rnavar.nf in the nf-core/rnavar pipeline -// - -class WorkflowRnavar { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - genomeExistsError(params, log) - - if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - System.exit(1) - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += " \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - log.error "=============================================================================\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "===================================================================================" - System.exit(1) - } - } -} diff --git a/lib/WorkflowSraDownload.groovy b/lib/WorkflowSraDownload.groovy deleted file mode 100755 index a1470c18..00000000 --- a/lib/WorkflowSraDownload.groovy +++ /dev/null @@ -1,21 +0,0 @@ -// -// This file holds functions specific to the workflow/sra_download.nf in the nf-core/rnaseq pipeline -// - -class WorkflowSraDownload { - - // - // Print a warning after SRA download has completed - // - public static void sraDownloadWarn(log) { - log.warn "=============================================================================\n" + - " Please double-check the samplesheet that has been auto-created using the\n" + - " public database ids provided via the '--public_data_ids' parameter.\n\n" + - " Public databases don't reliably hold information such as strandedness\n" + - " information.\n\n" + - " All of the sample metadata obtained from the ENA has been appended\n" + - " as additional columns to help you manually curate the samplesheet before\n" + - " you run the main workflow(s) in the pipeline.\n" + - "===================================================================================" - } -} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q 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https://github.com/nf-core/rnavar - Website: https://nf-co.re/rnavar - Slack : https://nfcore.slack.com/channels/rnavar ----------------------------------------------------------------------------------------- -*/ - -nextflow.enable.dsl = 2 - -/* -======================================================================================== - GENOME PARAMETER VALUES -======================================================================================== -*/ - -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') -params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') -params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') -params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') - -/* -======================================================================================== - VALIDATE & PRINT PARAMETER SUMMARY -======================================================================================== -*/ - -WorkflowMain.initialise(workflow, params, log) - -/* -======================================================================================== - NAMED WORKFLOW FOR PIPELINE -======================================================================================== -*/ - -include { RNAVAR } from './workflows/rnavar' - -// -// WORKFLOW: Run main nf-core/rnavar analysis pipeline -// -workflow NFCORE_RNAVAR { - RNAVAR () -} - -/* -======================================================================================== - RUN ALL WORKFLOWS -======================================================================================== -*/ - -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// -workflow { - NFCORE_RNAVAR () -} - -/* -======================================================================================== - THE END -======================================================================================== -*/ diff --git a/modules.json b/modules.json deleted file mode 100644 index df6c1f16..00000000 --- a/modules.json +++ /dev/null @@ -1,83 +0,0 @@ -{ - "name": "nf-core/rnavar", - "homePage": "https://github.com/nf-core/rnavar", - "repos": { - "nf-core/modules": { - "cat/fastq": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "fastqc": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "gatk4/baserecalibrator": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "gatk4/bedtointervallist": { - "git_sha": "d624336cc1d219ff96d3cf00ab5933d2276bbdac" - }, - "gatk4/createsequencedictionary": { - "git_sha": "c8877835c288eb32b2ad1810d38a78e83e791f3d" - }, - "gatk4/intervallisttools": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "gffread": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "gunzip": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "hisat2/build": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "hisat2/extractsplicesites": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "multiqc": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "picard/markduplicates": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "qualimap/bamqc": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "rsem/preparereference": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - }, - "salmon/index": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/faidx": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/flagstat": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/idxstats": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/index": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/merge": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/sort": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "samtools/stats": { - "git_sha": "c5235a983d454787fa0c3247b02086969217163b" - }, - "star/align": { - "git_sha": "5de3f2c50e063bda30316342b6606b485a12e38c" - }, - "star/genomegenerate": { - "git_sha": "f43778b0e641a9643ae73fe1f2fe2d5bb81edffc" - }, - "untar": { - "git_sha": "e937c7950af70930d1f34bb961403d9d2aa81c7d" - } - } - } -} diff --git a/modules/local/functions.nf b/modules/local/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/applybqsr/functions.nf b/modules/local/gatk4/applybqsr/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/applybqsr/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/applybqsr/main.nf b/modules/local/gatk4/applybqsr/main.nf deleted file mode 100644 index 8e10a5f2..00000000 --- a/modules/local/gatk4/applybqsr/main.nf +++ /dev/null @@ -1,46 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_APPLYBQSR { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervals) - path fasta - path fastaidx - path dict - - output: - tuple val(meta), path("*.bam"), emit: bam - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def interval = intervals ? "-L ${intervals}" : "" - """ - gatk ApplyBQSR \\ - -R $fasta \\ - -I $bam \\ - --bqsr-recal-file $bqsr_table \\ - $interval \\ - -O ${prefix}.bam \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/applybqsr/meta.yml b/modules/local/gatk4/applybqsr/meta.yml deleted file mode 100644 index 9bf12f09..00000000 --- a/modules/local/gatk4/applybqsr/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: gatk4_applybqsr -description: Apply base quality score recalibration (BQSR) to a bam file -keywords: - - bqsr - - bam -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file from alignment - pattern: "*.{bam}" - - bqsr_table: - type: file - description: Recalibration table from gatk4_baserecalibrator - - fasta: - type: file - description: The reference fasta file - - fastaidx: - type: file - description: Index of reference fasta file - - dict: - type: file - description: GATK sequence dictionary - - intervalsBed: - type: file - description: Bed file with the genomic regions included in the library (optional) - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - bam: - type: file - description: Recalibrated BAM file - pattern: "*.{bam}" - -authors: - - "@yocra3" diff --git a/modules/local/gatk4/haplotypecaller/functions.nf b/modules/local/gatk4/haplotypecaller/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/haplotypecaller/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf deleted file mode 100644 index da90ba4b..00000000 --- a/modules/local/gatk4/haplotypecaller/main.nf +++ /dev/null @@ -1,60 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_HAPLOTYPECALLER { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bam), path(bai), path(interval) - path dbsnp - path dbsnp_tbi - path dict - path fasta - path fai - val no_intervals - - output: - tuple val(meta), path("*.vcf") , emit: vcf - tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" - def avail_mem = 3 - if (!task.memory) { - log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - def intervalsOptions = no_intervals ? "" : "-L ${interval}" - def dbsnpOptions = dbsnp ? "-D ${dbsnp}" : "" - //TODO allow ploidy argument here since we allow it for the cnv callers? or is this covered with options? Might unintuitive to use - """ - gatk \\ - --java-options "-Xmx${avail_mem}g" \\ - HaplotypeCaller \\ - -R $fasta \\ - -I $bam \\ - ${dbsnpOptions} \\ - ${intervalsOptions} \\ - -O ${prefix}.vcf \\ - $options.args - - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/haplotypecaller/meta.yml b/modules/local/gatk4/haplotypecaller/meta.yml deleted file mode 100644 index f0fc3910..00000000 --- a/modules/local/gatk4/haplotypecaller/meta.yml +++ /dev/null @@ -1,63 +0,0 @@ -name: gatk4_haplotypecaller -description: Call germline SNPs and indels via local re-assembly of haplotypes -keywords: - - gatk4 - - haplotypecaller - - haplotype -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.bam" - - bai: - type: file - description: Index of BAM file - pattern: "*.bam.bai" - - fasta: - type: file - description: The reference fasta file - pattern: "*.fasta" - - fai: - type: file - description: Index of reference fasta file - pattern: "fasta.fai" - - dict: - type: file - description: GATK sequence dictionary - pattern: "*.dict" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - vcf: - type: file - description: Compressed VCF file - pattern: "*.vcf.gz" - - tbi: - type: file - description: Index of VCF file - pattern: "*.vcf.gz.tbi" - -authors: - - "@suzannejin" diff --git a/modules/local/gatk4/mergevcfs/functions.nf b/modules/local/gatk4/mergevcfs/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/mergevcfs/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/mergevcfs/main.nf b/modules/local/gatk4/mergevcfs/main.nf deleted file mode 100644 index 8b9747bc..00000000 --- a/modules/local/gatk4/mergevcfs/main.nf +++ /dev/null @@ -1,49 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_MERGEVCFS { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(vcfs) - path ref_dict - val use_ref_dict - - output: - tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi'), emit: vcf - path '*.version.txt' , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - - // Make list of VCFs to merge - def input = "" - for (vcf in vcfs) { - input += " I=${vcf}" - } - def ref = use_ref_dict ? "D=${ref_dict}" : "" - """ - gatk MergeVcfs \\ - $input \\ - O=${prefix}.vcf.gz \\ - $ref \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/mergevcfs/meta.yml b/modules/local/gatk4/mergevcfs/meta.yml deleted file mode 100644 index 14b28fa0..00000000 --- a/modules/local/gatk4/mergevcfs/meta.yml +++ /dev/null @@ -1,42 +0,0 @@ -name: gatk4_mergevcfs -description: Merges several vcf files -keywords: - - vcf - - merge -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - vcf: - type: list - description: Two or more VCF files - pattern: "*.{vcf,vcf.gz}" - - ref_dict: - type: file - description: Optional Sequence Dictionary as input - pattern: "*.dict" - - use_ref_dict: - type: boolean - description: Specify whether or not to use a given reference dictionary -output: - - vcf: - type: file - description: merged vcf file - pattern: "*.vcf.gz" - - version: - type: file - description: File containing software version - pattern: "*.version.txt" -authors: - - "@kevinmenden" diff --git a/modules/local/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/splitncigarreads/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/splitncigarreads/main.nf b/modules/local/gatk4/splitncigarreads/main.nf deleted file mode 100644 index d1c328ea..00000000 --- a/modules/local/gatk4/splitncigarreads/main.nf +++ /dev/null @@ -1,43 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_SPLITNCIGARREADS { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bam) - path(fasta) - path(fai) - path(dict) - - output: - tuple val(meta), path('*.bam'), path('*.bai'), emit: bam - path '*.version.txt' , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - gatk SplitNCigarReads \\ - -R $fasta \\ - -I $bam \\ - -O ${prefix}.bam \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml deleted file mode 100644 index e433cbf6..00000000 --- a/modules/local/gatk4/splitncigarreads/meta.yml +++ /dev/null @@ -1,40 +0,0 @@ -name: gatk4_splitncigarreads -description: Splits reads that contain Ns in their cigar string -keywords: - - vcf - - merge -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - bam: - type: list - description: BAM/SAM/CRAM file containing reads - pattern: "*.{bam,sam,cram}" - - fasta: - type: tuple of files - description: | - Tuple of fasta file (first), sequence dict (second) and fasta index (third) - pattern: ["*.fasta", "*.dict", "*.fai"] -output: - - bam: - type: file - description: Output file with split reads (BAM/SAM/CRAM) - pattern: "*.{bam,sam,cram}" - - version: - type: file - description: File containing software version - pattern: "*.version.txt" -authors: - - "@kevinmenden" diff --git a/modules/local/gatk4/variantfiltration/functions.nf b/modules/local/gatk4/variantfiltration/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/variantfiltration/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/variantfiltration/main.nf b/modules/local/gatk4/variantfiltration/main.nf deleted file mode 100644 index d442aba8..00000000 --- a/modules/local/gatk4/variantfiltration/main.nf +++ /dev/null @@ -1,44 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_VARIANTFILTRATION { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(vcf), path (tbi) - path fasta - path fai - path dict - - output: - tuple val(meta), path("*.vcf"), emit: vcf - path "*.version.txt" , emit: version - - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - gatk VariantFiltration \\ - -R $fasta \\ - -V $vcf \\ - -O ${prefix}.vcf \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/variantfiltration/meta.yml b/modules/local/gatk4/variantfiltration/meta.yml deleted file mode 100644 index d7f72582..00000000 --- a/modules/local/gatk4/variantfiltration/meta.yml +++ /dev/null @@ -1,47 +0,0 @@ -name: gatk4_variantfiltration -description: Filter variants -keywords: - - vcf - - filter -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - vcf: - type: list - description: Input VCF file - pattern: "*.{vcf}" - - fasta: - type: file - description: Fasta file of reference genome - pattern: "*.fasta" - - fai: - type: file - description: Index of fasta file - pattern: "*.fasta.fai" - - dict: - type: file - description: Sequence dictionary of fastea file - pattern: "*.dict" -output: - - vcf: - type: file - description: filtered VCF file - pattern: "*.filtered.{vcf}" - - version: - type: file - description: File containing software version - pattern: "*.version.txt" -authors: - - "@kevinmenden" diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf deleted file mode 100644 index 56c5718c..00000000 --- a/modules/local/get_chrom_sizes.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process GET_CHROM_SIZES { - tag "$fasta" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } - - input: - path fasta - - output: - path '*.sizes' , emit: sizes - path '*.fai' , emit: fai - path "*.version.txt", emit: version - - script: - def software = 'samtools' - """ - samtools faidx $fasta - cut -f 1,2 ${fasta}.fai > ${fasta}.sizes - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/get_software_versions.nf b/modules/local/get_software_versions.nf deleted file mode 100644 index d3d4e145..00000000 --- a/modules/local/get_software_versions.nf +++ /dev/null @@ -1,33 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process GET_SOFTWARE_VERSIONS { - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } - - cache false - - input: - path versions - - output: - path "software_versions.tsv" , emit: tsv - path 'software_versions_mqc.yaml', emit: yaml - - script: // This script is bundled with the pipeline, in nf-core/rnavar/bin/ - """ - echo $workflow.manifest.version > pipeline.version.txt - echo $workflow.nextflow.version > nextflow.version.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf deleted file mode 100644 index 9bc1d85e..00000000 --- a/modules/local/gtf2bed.nf +++ /dev/null @@ -1,30 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process GTF2BED { - tag "$gtf" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/perl:5.26.2" - } else { - container "quay.io/biocontainers/perl:5.26.2" - } - - input: - path gtf - - output: - path '*.bed' - - script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ - """ - gtf2bed $gtf > ${gtf.baseName}.bed - """ -} diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf deleted file mode 100644 index d30ab21b..00000000 --- a/modules/local/gtf_gene_filter.nf +++ /dev/null @@ -1,30 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process GTF_GENE_FILTER { - tag "$fasta" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } - - input: - path fasta - path gtf - - output: - path "*.gtf" - - script: // filter_gtf_for_genes_in_genome.py is bundled with the pipeline, in nf-core/rnaseq/bin/ - """ - filter_gtf_for_genes_in_genome.py --gtf $gtf --fasta $fasta -o ${fasta.baseName}_genes.gtf - """ -} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index bbad1ff0..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -// Import generic module functions -include { saveFiles } from './functions' - -params.options = [:] - -process SAMPLESHEET_CHECK { - tag "$samplesheet" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } - - input: - path samplesheet - - output: - path '*.csv' - - script: // This script is bundled with the pipeline, in nf-core/rnavar/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - """ -} diff --git a/modules/nf-core/modules/cat/fastq/functions.nf b/modules/nf-core/modules/cat/fastq/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/cat/fastq/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf deleted file mode 100644 index 55ccca90..00000000 --- a/modules/nf-core/modules/cat/fastq/main.nf +++ /dev/null @@ -1,47 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process CAT_FASTQ { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.merged.fastq.gz"), emit: reads - - script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def readList = reads.collect{ it.toString() } - if (meta.single_end) { - if (readList.size > 1) { - """ - cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz - """ - } - } else { - if (readList.size > 2) { - def read1 = [] - def read2 = [] - readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } - """ - cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz - cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz - """ - } - } -} diff --git a/modules/nf-core/modules/cat/fastq/meta.yml b/modules/nf-core/modules/cat/fastq/meta.yml deleted file mode 100644 index e7b8eebe..00000000 --- a/modules/nf-core/modules/cat/fastq/meta.yml +++ /dev/null @@ -1,33 +0,0 @@ -name: cat_fastq -description: Concatenates fastq files -keywords: - - fastq - - concatenate -tools: - - cat: - description: | - The cat utility reads files sequentially, writing them to the standard output. - documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: list - description: | - List of input FastQ files to be concatenated. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: Merged fastq file - pattern: "*.{merged.fastq.gz}" -authors: - - "@joseespinosa" - - "@drpatelh" diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/fastqc/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf deleted file mode 100644 index 39c327b2..00000000 --- a/modules/nf-core/modules/fastqc/main.nf +++ /dev/null @@ -1,47 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process FASTQC { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" - } else { - container "quay.io/biocontainers/fastqc:0.11.9--0" - } - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "*.version.txt" , emit: version - - script: - // Add soft-links to original FastQs for consistent naming in pipeline - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - if (meta.single_end) { - """ - [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz - fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz - fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt - """ - } else { - """ - [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz - [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz - fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz - fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt - """ - } -} diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml deleted file mode 100644 index 8eb9953d..00000000 --- a/modules/nf-core/modules/fastqc/meta.yml +++ /dev/null @@ -1,51 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf b/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf deleted file mode 100644 index 4e2730f2..00000000 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ /dev/null @@ -1,50 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_BASERECALIBRATOR { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bam), path(bai) - path fasta - path fastaidx - path dict - path intervalsBed - path knownSites - path knownSites_tbi - - output: - tuple val(meta), path("*.table"), emit: table - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" - def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') - """ - gatk BaseRecalibrator \ - -R $fasta \ - -I $bam \ - $sitesCommand \ - $intervalsCommand \ - $options.args \ - -O ${prefix}.table - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml deleted file mode 100644 index 0996dcbe..00000000 --- a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: gatk4_baserecalibrator -description: Generate recalibration table for Base Quality Score Recalibration (BQSR) -keywords: - - sort -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file from alignment - pattern: "*.{bam}" - - fasta: - type: file - description: The reference fasta file - - fastaidx: - type: file - description: Index of reference fasta file - - dict: - type: file - description: GATK sequence dictionary - - intervalsBed: - type: file - description: Bed file with the genomic regions included in the library (optional) - - knownSites: - type: file - description: Bed file with the genomic regions included in the library (optional) - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - table: - type: file - description: Recalibration table from BaseRecalibrator - pattern: "*.{table}" - -authors: - - "@yocra3" diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf deleted file mode 100644 index af385f8f..00000000 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ /dev/null @@ -1,41 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_BEDTOINTERVALLIST { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bed) - path sequence_dict - - output: - tuple val(meta), path('*.interval_list'), emit: interval_list - path '*.version.txt' , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - gatk BedToIntervalList \\ - -I $bed \\ - -SD $sequence_dict \\ - -O ${prefix}.interval_list \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml b/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml deleted file mode 100644 index 23e98447..00000000 --- a/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml +++ /dev/null @@ -1,39 +0,0 @@ -name: gatk4_bedtointervallist -description: Creates an interval list from a bed file and a reference dict -keywords: - - bed - - interval list -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - bed: - type: file - description: Input bed file - pattern: "*.bed" - - dict: - type: file - description: Sequence dictionary - pattern: "*.dict" -output: - - interval_list: - type: file - description: gatk interval list file - pattern: "*.interval_list" - - version: - type: file - description: File containing software version - pattern: "*.version.txt" -authors: - - "@kevinmenden" diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf deleted file mode 100644 index 0276e8b2..00000000 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ /dev/null @@ -1,45 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_CREATESEQUENCEDICTIONARY { - tag "$fasta" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - path fasta - - output: - path "*.dict" , emit: dict - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def avail_mem = 6 - if (!task.memory) { - log.info '[GATK] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - """ - gatk --java-options "-Xmx${avail_mem}g" \\ - CreateSequenceDictionary \\ - --REFERENCE $fasta \\ - --URI $fasta \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml deleted file mode 100644 index d0cc5980..00000000 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml +++ /dev/null @@ -1,30 +0,0 @@ -name: gatk4_createsequencedictionary -description: Creates a sequence dictionary for a reference sequence -keywords: - - dictionary - - fasta -tools: - - gatk: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - fasta: - type: file - description: Input fasta file - pattern: "*.{fasta,fa}" -output: - - dict: - type: file - description: gatk dictionary file - pattern: "*.{dict}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@maxulysse" diff --git a/modules/nf-core/modules/gatk4/intervallisttools/functions.nf b/modules/nf-core/modules/gatk4/intervallisttools/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gatk4/intervallisttools/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf deleted file mode 100644 index b2486eac..00000000 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ /dev/null @@ -1,53 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_INTERVALLISTTOOLS { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--hdfd78af_1" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--hdfd78af_1" - } - - input: - tuple val(meta), path(interval_list) - - output: - tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - - mkdir ${prefix}_split - - gatk \\ - IntervalListTools \\ - -I ${interval_list} \\ - -O ${prefix}_split \\ - $options.args - - python3 <&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gatk4/intervallisttools/meta.yml b/modules/nf-core/modules/gatk4/intervallisttools/meta.yml deleted file mode 100644 index efe31da1..00000000 --- a/modules/nf-core/modules/gatk4/intervallisttools/meta.yml +++ /dev/null @@ -1,46 +0,0 @@ -name: gatk4_intervallisttools - -description: Splits the interval list file into unique, equally-sized interval files and place it under a directory -keywords: - - sort - - bed - - interval list -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - - interval_list: - type: file - description: Interval list file - pattern: "*.interval_list" - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - interval_list: - type: file - description: Interval list files - pattern: "*.interval_list" - -authors: - - "@praveenraj2018" diff --git a/modules/nf-core/modules/gffread/functions.nf b/modules/nf-core/modules/gffread/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gffread/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gffread/main.nf b/modules/nf-core/modules/gffread/main.nf deleted file mode 100644 index 6c5aecbc..00000000 --- a/modules/nf-core/modules/gffread/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GFFREAD { - tag "$gff" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "bioconda::gffread=0.12.1" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0" - } else { - container "quay.io/biocontainers/gffread:0.12.1--h8b12597_0" - } - - input: - path gff - - output: - path "*.gtf" , emit: gtf - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - """ - gffread $gff $options.args -o ${gff.baseName}.gtf - echo \$(gffread --version 2>&1) > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gffread/meta.yml b/modules/nf-core/modules/gffread/meta.yml deleted file mode 100644 index 63d281f5..00000000 --- a/modules/nf-core/modules/gffread/meta.yml +++ /dev/null @@ -1,43 +0,0 @@ -name: gffread -description: Validate, filter, convert and perform various other operations on GFF files -keywords: - - gff - - conversion - - validation -tools: - - gffread: - description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more. - homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread - documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread - tool_dev_url: https://github.com/gpertea/gffread - doi: 10.12688/f1000research.23297.1 - licence: ['MIT'] - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - gff: - type: file - description: A GFF file in either the GFF3 or GFF2 format. - patter: "*.gff" - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - gtf: - type: file - description: GTF file resulting from the conversion of the GFF input file - pattern: "*.gtf" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - -authors: - - "@emiller88" diff --git a/modules/nf-core/modules/gunzip/functions.nf b/modules/nf-core/modules/gunzip/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/gunzip/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf deleted file mode 100644 index 29248796..00000000 --- a/modules/nf-core/modules/gunzip/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GUNZIP { - tag "$archive" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } - - input: - path archive - - output: - path "$gunzip", emit: gunzip - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - gunzip = archive.toString() - '.gz' - """ - gunzip -f $options.args $archive - echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml deleted file mode 100644 index 922e74e6..00000000 --- a/modules/nf-core/modules/gunzip/meta.yml +++ /dev/null @@ -1,27 +0,0 @@ -name: gunzip -description: Compresses and decompresses files. -keywords: - - gunzip - - compression -tools: - - gunzip: - description: | - gzip is a file format and a software application used for file compression and decompression. - documentation: https://www.gnu.org/software/gzip/manual/gzip.html -input: - - archive: - type: file - description: File to be compressed/uncompressed - pattern: "*.*" -output: - - gunzip: - type: file - description: Compressed/uncompressed file - pattern: "*.*" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@joseespinosa" - - "@drpatelh" diff --git a/modules/nf-core/modules/hisat2/build/functions.nf b/modules/nf-core/modules/hisat2/build/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/hisat2/build/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/hisat2/build/main.nf b/modules/nf-core/modules/hisat2/build/main.nf deleted file mode 100644 index 3e74b1d4..00000000 --- a/modules/nf-core/modules/hisat2/build/main.nf +++ /dev/null @@ -1,70 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -def VERSION = '2.2.0' - -process HISAT2_BUILD { - tag "$fasta" - label 'process_high' - label 'process_high_memory' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? 'bioconda::hisat2=2.2.1' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/hisat2:2.2.1--h1b792b2_3" - } else { - container "quay.io/biocontainers/hisat2:2.2.1--h1b792b2_3" - } - - input: - path fasta - path gtf - path splicesites - - output: - path "hisat2" , emit: index - path "*.version.txt", emit: version - - script: - def avail_mem = 0 - if (!task.memory) { - log.info "[HISAT2 index build] Available memory not known - defaulting to 0. Specify process memory requirements to change this." - } else { - log.info "[HISAT2 index build] Available memory: ${task.memory}" - avail_mem = task.memory.toGiga() - } - - def ss = '' - def exon = '' - def extract_exons = '' - def hisat2_build_memory = params.hisat2_build_memory ? (params.hisat2_build_memory as nextflow.util.MemoryUnit).toGiga() : 0 - if (avail_mem >= hisat2_build_memory) { - log.info "[HISAT2 index build] At least ${hisat2_build_memory} GB available, so using splice sites and exons to build HISAT2 index" - extract_exons = "hisat2_extract_exons.py $gtf > ${gtf.baseName}.exons.txt" - ss = "--ss $splicesites" - exon = "--exon ${gtf.baseName}.exons.txt" - } else { - log.info "[HISAT2 index build] Less than ${hisat2_build_memory} GB available, so NOT using splice sites and exons to build HISAT2 index." - log.info "[HISAT2 index build] Use --hisat2_build_memory [small number] to skip this check." - } - - def software = getSoftwareName(task.process) - """ - mkdir hisat2 - $extract_exons - hisat2-build \\ - -p $task.cpus \\ - $ss \\ - $exon \\ - $options.args \\ - $fasta \\ - hisat2/${fasta.baseName} - - echo $VERSION > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/hisat2/build/meta.yml b/modules/nf-core/modules/hisat2/build/meta.yml deleted file mode 100644 index 2f34d9d8..00000000 --- a/modules/nf-core/modules/hisat2/build/meta.yml +++ /dev/null @@ -1,42 +0,0 @@ -name: hisat2_build -description: Builds HISAT2 index for reference genome -keywords: - - build - - index - - fasta - - genome - - reference -tools: - - hisat2: - description: HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome. - homepage: https://daehwankimlab.github.io/hisat2/ - documentation: https://daehwankimlab.github.io/hisat2/manual/ - doi: "10.1038/s41587-019-0201-4" - licence: ['MIT'] - -input: - - fasta: - type: file - description: Reference fasta file - pattern: "*.{fa,fasta,fna}" - - gtf: - type: file - description: Reference gtf annotation file - pattern: "*.{gtf}" - - splicesites: - type: file - description: Splices sites in gtf file - pattern: "*.{txt}" - -output: - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - index: - type: file - description: HISAT2 genome index file - pattern: "*.ht2" - -authors: - - "@ntoda03" diff --git a/modules/nf-core/modules/hisat2/extractsplicesites/functions.nf b/modules/nf-core/modules/hisat2/extractsplicesites/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/hisat2/extractsplicesites/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/hisat2/extractsplicesites/main.nf b/modules/nf-core/modules/hisat2/extractsplicesites/main.nf deleted file mode 100644 index d97fdb89..00000000 --- a/modules/nf-core/modules/hisat2/extractsplicesites/main.nf +++ /dev/null @@ -1,36 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -def VERSION = '2.2.0' - -process HISAT2_EXTRACTSPLICESITES { - tag "$gtf" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? 'bioconda::hisat2=2.2.1' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/hisat2:2.2.1--h1b792b2_3" - } else { - container "quay.io/biocontainers/hisat2:2.2.1--h1b792b2_3" - } - - input: - path gtf - - output: - path "*.splice_sites.txt", emit: txt - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - hisat2_extract_splice_sites.py $gtf > ${gtf.baseName}.splice_sites.txt - echo $VERSION > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/hisat2/extractsplicesites/meta.yml b/modules/nf-core/modules/hisat2/extractsplicesites/meta.yml deleted file mode 100644 index 228138a8..00000000 --- a/modules/nf-core/modules/hisat2/extractsplicesites/meta.yml +++ /dev/null @@ -1,34 +0,0 @@ -name: hisat2_extractsplicesites -description: Extracts splicing sites from a gtf files -keywords: - - splicing - - gtf - - genome - - reference - -tools: - - hisat2: - description: HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes as well as to a single reference genome. - homepage: https://daehwankimlab.github.io/hisat2/ - documentation: https://daehwankimlab.github.io/hisat2/manual/ - doi: "10.1038/s41587-019-0201-4" - licence: ['MIT'] - -input: - - gtf: - type: file - description: Reference gtf annotation file - pattern: "*.{gtf}" - -output: - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - splicesites: - type: file - description: Splices sites in gtf file - pattern: "*.{splice_sites.txt}" - -authors: - - "@ntoda03" diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/multiqc/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf deleted file mode 100644 index 8b6d6f0c..00000000 --- a/modules/nf-core/modules/multiqc/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process MULTIQC { - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" - } else { - container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" - } - - input: - path multiqc_files - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - multiqc -f $options.args . - multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml deleted file mode 100644 index 532a8bb1..00000000 --- a/modules/nf-core/modules/multiqc/meta.yml +++ /dev/null @@ -1,39 +0,0 @@ -name: MultiQC -description: Aggregate results from bioinformatics analyses across many samples into a single report -keywords: - - QC - - bioinformatics tools - - Beautiful stand-alone HTML report -tools: - - multiqc: - description: | - MultiQC searches a given directory for analysis logs and compiles a HTML report. - It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. - homepage: https://multiqc.info/ - documentation: https://multiqc.info/docs/ -input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC -output: - - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" - - data: - type: dir - description: MultiQC data dir - pattern: "multiqc_data" - - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@abhi18av" - - "@bunop" - - "@drpatelh" diff --git a/modules/nf-core/modules/picard/markduplicates/functions.nf b/modules/nf-core/modules/picard/markduplicates/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/picard/markduplicates/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf deleted file mode 100644 index d20014bf..00000000 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ /dev/null @@ -1,49 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process PICARD_MARKDUPLICATES { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::picard=2.25.7' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/picard:2.25.7--hdfd78af_0" - } else { - container "quay.io/biocontainers/picard:2.25.7--hdfd78af_0" - } - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bam") , emit: bam - tuple val(meta), path("*.metrics.txt"), emit: metrics - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def avail_mem = 3 - if (!task.memory) { - log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - """ - picard \\ - -Xmx${avail_mem}g \\ - MarkDuplicates \\ - $options.args \\ - INPUT=$bam \\ - OUTPUT=${prefix}.bam \\ - METRICS_FILE=${prefix}.MarkDuplicates.metrics.txt - - echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d: > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/picard/markduplicates/meta.yml b/modules/nf-core/modules/picard/markduplicates/meta.yml deleted file mode 100644 index 6420ce9a..00000000 --- a/modules/nf-core/modules/picard/markduplicates/meta.yml +++ /dev/null @@ -1,46 +0,0 @@ -name: picard_markduplicates -description: Locate and tag duplicate reads in a BAM file -keywords: - - markduplicates - - pcr - - duplicates - - bam - - sam - - cram -tools: - - picard: - description: | - A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) - data and formats such as SAM/BAM/CRAM and VCF. - homepage: https://broadinstitute.github.io/picard/ - documentation: https://broadinstitute.github.io/picard/ -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.{bam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file with duplicate reads marked/removed - pattern: "*.{bam}" - - metrics: - type: file - description: Duplicate metrics file generated by picard - pattern: "*.{metrics.txt}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" diff --git a/modules/nf-core/modules/qualimap/bamqc/functions.nf b/modules/nf-core/modules/qualimap/bamqc/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/qualimap/bamqc/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/qualimap/bamqc/main.nf b/modules/nf-core/modules/qualimap/bamqc/main.nf deleted file mode 100644 index 463cde4c..00000000 --- a/modules/nf-core/modules/qualimap/bamqc/main.nf +++ /dev/null @@ -1,61 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process QUALIMAP_BAMQC { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::qualimap=2.2.2d" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1" - } else { - container "quay.io/biocontainers/qualimap:2.2.2d--1" - } - - input: - tuple val(meta), path(bam) - path gff - val use_gff - - output: - tuple val(meta), path("${prefix}"), emit: results - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - - def collect_pairs = meta.single_end ? '' : '--collect-overlap-pairs' - def memory = task.memory.toGiga() + "G" - def regions = use_gff ? "--gff $gff" : '' - - def strandedness = 'non-strand-specific' - if (meta.strandedness == 'forward') { - strandedness = 'strand-specific-forward' - } else if (meta.strandedness == 'reverse') { - strandedness = 'strand-specific-reverse' - } - """ - unset DISPLAY - mkdir tmp - export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp - qualimap \\ - --java-mem-size=$memory \\ - bamqc \\ - $options.args \\ - -bam $bam \\ - $regions \\ - -p $strandedness \\ - $collect_pairs \\ - -outdir $prefix \\ - -nt $task.cpus - - echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/qualimap/bamqc/meta.yml b/modules/nf-core/modules/qualimap/bamqc/meta.yml deleted file mode 100644 index 3c608f31..00000000 --- a/modules/nf-core/modules/qualimap/bamqc/meta.yml +++ /dev/null @@ -1,49 +0,0 @@ -name: qualimap_bamqc -description: Evaluate alignment data -keywords: - - quality control - - qc - - bam -tools: - - qualimap: - description: | - Qualimap 2 is a platform-independent application written in - Java and R that provides both a Graphical User Interface and - a command-line interface to facilitate the quality control of - alignment sequencing data and its derivatives like feature counts. - homepage: http://qualimap.bioinfo.cipf.es/ - documentation: http://qualimap.conesalab.org/doc_html/index.html - doi: 10.1093/bioinformatics/bts503 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.{bam}" - - gff: - type: file - description: Feature file with regions of interest - pattern: "*.{gff,gtf,bed}" - - use_gff: - type: boolean - description: Specifies if feature file should be used or not -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - results: - type: dir - description: Qualimap results dir - pattern: "*/*" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@phue" diff --git a/modules/nf-core/modules/rsem/preparereference/functions.nf b/modules/nf-core/modules/rsem/preparereference/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/rsem/preparereference/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/rsem/preparereference/main.nf b/modules/nf-core/modules/rsem/preparereference/main.nf deleted file mode 100644 index a378eb6b..00000000 --- a/modules/nf-core/modules/rsem/preparereference/main.nf +++ /dev/null @@ -1,67 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process RSEM_PREPAREREFERENCE { - tag "$fasta" - label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" - } else { - container "quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0" - } - - input: - path fasta, stageAs: "rsem/*" - path gtf - - output: - path "rsem" , emit: index - path "rsem/*transcripts.fa", emit: transcript_fasta - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def args = options.args.tokenize() - if (args.contains('--star')) { - args.removeIf { it.contains('--star') } - def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' - """ - STAR \\ - --runMode genomeGenerate \\ - --genomeDir rsem/ \\ - --genomeFastaFiles $fasta \\ - --sjdbGTFfile $gtf \\ - --runThreadN $task.cpus \\ - $memory \\ - $options.args2 - - rsem-prepare-reference \\ - --gtf $gtf \\ - --num-threads $task.cpus \\ - ${args.join(' ')} \\ - $fasta \\ - rsem/genome - - rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt - """ - } else { - """ - rsem-prepare-reference \\ - --gtf $gtf \\ - --num-threads $task.cpus \\ - $options.args \\ - $fasta \\ - rsem/genome - - rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt - """ - } -} diff --git a/modules/nf-core/modules/rsem/preparereference/meta.yml b/modules/nf-core/modules/rsem/preparereference/meta.yml deleted file mode 100644 index d7c02154..00000000 --- a/modules/nf-core/modules/rsem/preparereference/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -name: rsem_preparereference -description: Prepare a reference genome for RSEM -keywords: - - rsem - - reference -tools: - - rseqc: - description: | - RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome - homepage: https://github.com/deweylab/RSEM - documentation: https://github.com/deweylab/RSEM - doi: https://doi.org/10.1186/1471-2105-12-323 -input: - - fasta: - type: file - description: The Fasta file of the reference genome - pattern: "*.{fasta,fa}" - - gtf: - type: file - description: The GTF file of the reference genome - pattern: "*.gtf" -output: - - rsem: - type: directory - description: RSEM index directory - pattern: "rsem" - - transcript_fasta: - type: file - description: Fasta file of transcripts - pattern: "rsem/*transcripts.fa" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@kevinmenden" diff --git a/modules/nf-core/modules/salmon/index/functions.nf b/modules/nf-core/modules/salmon/index/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/salmon/index/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/salmon/index/main.nf b/modules/nf-core/modules/salmon/index/main.nf deleted file mode 100644 index e72ff121..00000000 --- a/modules/nf-core/modules/salmon/index/main.nf +++ /dev/null @@ -1,51 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SALMON_INDEX { - tag "$transcript_fasta" - label "process_medium" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? 'bioconda::salmon=1.5.2' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/salmon:1.5.2--h84f40af_0" - } else { - container "quay.io/biocontainers/salmon:1.5.2--h84f40af_0" - } - - input: - path genome_fasta - path transcript_fasta - - output: - path "salmon" , emit: index - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - def get_decoy_ids = "grep '^>' $genome_fasta | cut -d ' ' -f 1 > decoys.txt" - def gentrome = "gentrome.fa" - if (genome_fasta.endsWith('.gz')) { - get_decoy_ids = "grep '^>' <(gunzip -c $genome_fasta) | cut -d ' ' -f 1 > decoys.txt" - gentrome = "gentrome.fa.gz" - } - """ - $get_decoy_ids - sed -i.bak -e 's/>//g' decoys.txt - cat $transcript_fasta $genome_fasta > $gentrome - - salmon \\ - index \\ - --threads $task.cpus \\ - -t $gentrome \\ - -d decoys.txt \\ - $options.args \\ - -i salmon - salmon --version | sed -e "s/salmon //g" > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/salmon/index/meta.yml b/modules/nf-core/modules/salmon/index/meta.yml deleted file mode 100644 index 652e2a77..00000000 --- a/modules/nf-core/modules/salmon/index/meta.yml +++ /dev/null @@ -1,35 +0,0 @@ -name: salmon_index -description: Create index for salmon -keywords: - - index - - fasta - - genome - - reference -tools: - - salmon: - description: | - Salmon is a tool for wicked-fast transcript quantification from RNA-seq data - homepage: https://salmon.readthedocs.io/en/latest/salmon.html - manual: https://salmon.readthedocs.io/en/latest/salmon.html - doi: 10.1038/nmeth.4197 -input: - - genome_fasta: - type: file - description: Fasta file of the reference genome - - transcriptome_fasta: - type: file - description: Fasta file of the reference transcriptome - -output: - - index: - type: directory - description: Folder containing the star index files - pattern: "salmon" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - -authors: - - "@kevinmenden" - - "@drpatelh" diff --git a/modules/nf-core/modules/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/faidx/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf deleted file mode 100644 index a89ff2bb..00000000 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_FAIDX { - tag "$fasta" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - path fasta - - output: - path "*.fai" , emit: fai - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - """ - samtools faidx $fasta - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml deleted file mode 100644 index f92234d0..00000000 --- a/modules/nf-core/modules/samtools/faidx/meta.yml +++ /dev/null @@ -1,32 +0,0 @@ -name: samtools_faidx -description: Index FASTA file -keywords: - - index - - fasta -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: http://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - fasta: - type: file - description: FASTA file - pattern: "*.{fa,fasta}" -output: - - fai: - type: file - description: FASTA index file - pattern: "*.{fai}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@ewels" - - "@phue" diff --git a/modules/nf-core/modules/samtools/flagstat/functions.nf b/modules/nf-core/modules/samtools/flagstat/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/flagstat/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf deleted file mode 100644 index d4852c66..00000000 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_FLAGSTAT { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bam), path(bai) - - output: - tuple val(meta), path("*.flagstat"), emit: flagstat - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - samtools flagstat $bam > ${bam}.flagstat - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/flagstat/meta.yml b/modules/nf-core/modules/samtools/flagstat/meta.yml deleted file mode 100644 index 8414bf54..00000000 --- a/modules/nf-core/modules/samtools/flagstat/meta.yml +++ /dev/null @@ -1,48 +0,0 @@ -name: samtools_flagstat -description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG type -keywords: - - stats - - mapping - - counts - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - bai: - type: file - description: Index for BAM/CRAM/SAM file - pattern: "*.{bai,crai,sai}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - flagstat: - type: file - description: File containing samtools flagstat output - pattern: "*.{flagstat}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" diff --git a/modules/nf-core/modules/samtools/idxstats/functions.nf b/modules/nf-core/modules/samtools/idxstats/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/idxstats/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf deleted file mode 100644 index 14a07cfb..00000000 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_IDXSTATS { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bam), path(bai) - - output: - tuple val(meta), path("*.idxstats"), emit: idxstats - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - samtools idxstats $bam > ${bam}.idxstats - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/idxstats/meta.yml b/modules/nf-core/modules/samtools/idxstats/meta.yml deleted file mode 100644 index 530d0772..00000000 --- a/modules/nf-core/modules/samtools/idxstats/meta.yml +++ /dev/null @@ -1,49 +0,0 @@ -name: samtools_idxstats -description: Reports alignment summary statistics for a BAM/CRAM/SAM file -keywords: - - stats - - mapping - - counts - - chromosome - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - bai: - type: file - description: Index for BAM/CRAM/SAM file - pattern: "*.{bai,crai,sai}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - idxstats: - type: file - description: File containing samtools idxstats output - pattern: "*.{idxstats}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" diff --git a/modules/nf-core/modules/samtools/index/functions.nf b/modules/nf-core/modules/samtools/index/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/index/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf deleted file mode 100644 index e1966fb3..00000000 --- a/modules/nf-core/modules/samtools/index/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_INDEX { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bai"), optional:true, emit: bai - tuple val(meta), path("*.csi"), optional:true, emit: csi - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - samtools index $options.args $bam - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/index/meta.yml b/modules/nf-core/modules/samtools/index/meta.yml deleted file mode 100644 index 5d076e3b..00000000 --- a/modules/nf-core/modules/samtools/index/meta.yml +++ /dev/null @@ -1,47 +0,0 @@ -name: samtools_index -description: Index SAM/BAM/CRAM file -keywords: - - index - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bai: - type: file - description: BAM/CRAM/SAM index file - pattern: "*.{bai,crai,sai}" - - csi: - type: file - description: CSI index file - pattern: "*.{csi}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@ewels" diff --git a/modules/nf-core/modules/samtools/merge/functions.nf b/modules/nf-core/modules/samtools/merge/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/merge/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf deleted file mode 100644 index 0182b9fd..00000000 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_MERGE { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bams) - - output: - tuple val(meta), path("${prefix}.bam"), emit: bam - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - samtools merge ${prefix}.bam $bams - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/merge/meta.yml b/modules/nf-core/modules/samtools/merge/meta.yml deleted file mode 100644 index 9092f22e..00000000 --- a/modules/nf-core/modules/samtools/merge/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -name: samtools_merge -description: Merge BAM file -keywords: - - merge - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.{bam,cram,sam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - merged_bam: - type: file - description: BAM file - pattern: "*.{bam}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@yuukiiwa " - - "@maxulysse" diff --git a/modules/nf-core/modules/samtools/sort/functions.nf b/modules/nf-core/modules/samtools/sort/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/sort/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf deleted file mode 100644 index 0a6b7048..00000000 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_SORT { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bam"), emit: bam - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - samtools sort $options.args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml deleted file mode 100644 index 704e8c1f..00000000 --- a/modules/nf-core/modules/samtools/sort/meta.yml +++ /dev/null @@ -1,43 +0,0 @@ -name: samtools_sort -description: Sort SAM/BAM/CRAM file -keywords: - - sort - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: Sorted BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" - - "@ewels" diff --git a/modules/nf-core/modules/samtools/stats/functions.nf b/modules/nf-core/modules/samtools/stats/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/samtools/stats/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf deleted file mode 100644 index 8c72d725..00000000 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process SAMTOOLS_STATS { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" - } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" - } - - input: - tuple val(meta), path(bam), path(bai) - - output: - tuple val(meta), path("*.stats"), emit: stats - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - """ - samtools stats $bam > ${bam}.stats - echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/samtools/stats/meta.yml b/modules/nf-core/modules/samtools/stats/meta.yml deleted file mode 100644 index b549ff5c..00000000 --- a/modules/nf-core/modules/samtools/stats/meta.yml +++ /dev/null @@ -1,47 +0,0 @@ -name: samtools_stats -description: Produces comprehensive statistics from SAM/BAM/CRAM file -keywords: - - statistics - - counts - - bam - - sam - - cram -tools: - - samtools: - description: | - SAMtools is a set of utilities for interacting with and post-processing - short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. - These files are generated as output by short read aligners like BWA. - homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html - doi: 10.1093/bioinformatics/btp352 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM/CRAM/SAM file - pattern: "*.{bam,cram,sam}" - - bai: - type: file - description: Index for BAM/CRAM/SAM file - pattern: "*.{bai,crai,sai}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - stats: - type: file - description: File containing samtools stats output - pattern: "*.{stats}" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@drpatelh" diff --git a/modules/nf-core/modules/star/align/functions.nf b/modules/nf-core/modules/star/align/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/star/align/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf deleted file mode 100644 index d5d88ce8..00000000 --- a/modules/nf-core/modules/star/align/main.nf +++ /dev/null @@ -1,73 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process STAR_ALIGN { - tag "$meta.id" - label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' - } else { - container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' - } - - input: - tuple val(meta), path(reads) - path index - path gtf - - output: - tuple val(meta), path('*d.out.bam') , emit: bam - tuple val(meta), path('*Log.final.out') , emit: log_final - tuple val(meta), path('*Log.out') , emit: log_out - tuple val(meta), path('*Log.progress.out'), emit: log_progress - path '*.version.txt' , emit: version - - tuple val(meta), path('*sortedByCoord.out.bam') , optional:true, emit: bam_sorted - tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript - tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted - tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq - tuple val(meta), path('*.tab') , optional:true, emit: tab - tuple val(meta), path('*.out.junction') , optional:true, emit: junction - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" - def seq_platform = params.seq_platform ? "'PL:$params.seq_platform'" : "" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform " - def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' - def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' - """ - STAR \\ - --genomeDir $index \\ - --readFilesIn $reads \\ - --runThreadN $task.cpus \\ - --outFileNamePrefix $prefix. \\ - $out_sam_type \\ - $ignore_gtf \\ - $seq_center \\ - $options.args - - $mv_unsorted_bam - - if [ -f ${prefix}.Unmapped.out.mate1 ]; then - mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq - gzip ${prefix}.unmapped_1.fastq - fi - if [ -f ${prefix}.Unmapped.out.mate2 ]; then - mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq - gzip ${prefix}.unmapped_2.fastq - fi - - STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml deleted file mode 100644 index a589d145..00000000 --- a/modules/nf-core/modules/star/align/meta.yml +++ /dev/null @@ -1,80 +0,0 @@ -name: star_align -description: Align reads to a reference genome using STAR -keywords: - - align - - fasta - - genome - - reference -tools: - - star: - description: | - STAR is a software package for mapping DNA sequences against - a large reference genome, such as the human genome. - homepage: https://github.com/alexdobin/STAR - manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf - doi: 10.1093/bioinformatics/bts635 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - index: - type: directory - description: STAR genome index - pattern: "star" -output: - - bam: - type: file - description: Output BAM file containing read alignments - pattern: "*.{bam}" - - log_final: - type: file - description: STAR final log file - pattern: "*Log.final.out" - - log_out: - type: file - description: STAR lot out file - pattern: "*Log.out" - - log_progress: - type: file - description: STAR log progress file - pattern: "*Log.progress.out" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - bam_sorted: - type: file - description: Sorted BAM file of read alignments (optional) - pattern: "*sortedByCoord.out.bam" - - bam_transcript: - type: file - description: Output BAM file of transcriptome alignment (optional) - pattern: "*toTranscriptome.out.bam" - - bam_unsorted: - type: file - description: Unsorted BAM file of read alignments (optional) - pattern: "*Aligned.unsort.out.bam" - - fastq: - type: file - description: Unmapped FastQ files (optional) - pattern: "*fastq.gz" - - tab: - type: file - description: STAR output tab file(s) (optional) - pattern: "*.tab" - - junction: - type: file - description: STAR chimeric junction output file (optional) - pattern: "*.out.junction" - -authors: - - "@kevinmenden" - - "@drpatelh" - - "@praveenraj2018" diff --git a/modules/nf-core/modules/star/genomegenerate/functions.nf b/modules/nf-core/modules/star/genomegenerate/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/star/genomegenerate/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf deleted file mode 100644 index 9335b9b5..00000000 --- a/modules/nf-core/modules/star/genomegenerate/main.nf +++ /dev/null @@ -1,67 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process STAR_GENOMEGENERATE { - tag "$fasta" - label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } - - // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" - } else { - container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" - } - - input: - path fasta - path gtf - - output: - path "star" , emit: index - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' - def args = options.args.tokenize() - if (args.contains('--genomeSAindexNbases')) { - """ - mkdir star - STAR \\ - --runMode genomeGenerate \\ - --genomeDir star/ \\ - --genomeFastaFiles $fasta \\ - --sjdbGTFfile $gtf \\ - --runThreadN $task.cpus \\ - $memory \\ - $options.args - - STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt - """ - } else { - """ - samtools faidx $fasta - NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai` - - mkdir star - STAR \\ - --runMode genomeGenerate \\ - --genomeDir star/ \\ - --genomeFastaFiles $fasta \\ - --sjdbGTFfile $gtf \\ - --runThreadN $task.cpus \\ - --genomeSAindexNbases \$NUM_BASES \\ - $memory \\ - $options.args - - STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt - """ - } -} diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml deleted file mode 100644 index aae0219b..00000000 --- a/modules/nf-core/modules/star/genomegenerate/meta.yml +++ /dev/null @@ -1,36 +0,0 @@ -name: star_genomegenerate -description: Create index for STAR -keywords: - - index - - fasta - - genome - - reference -tools: - - star: - description: | - STAR is a software package for mapping DNA sequences against - a large reference genome, such as the human genome. - homepage: https://github.com/alexdobin/STAR - manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf - doi: 10.1093/bioinformatics/bts635 -input: - - fasta: - type: file - description: Fasta file of the reference genome - - gtf: - type: file - description: GTF file of the reference genome - -output: - - index: - type: directory - description: Folder containing the star index files - pattern: "star" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - -authors: - - "@kevinmenden" - - "@drpatelh" diff --git a/modules/nf-core/modules/untar/functions.nf b/modules/nf-core/modules/untar/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/nf-core/modules/untar/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf deleted file mode 100644 index fc6d7ec5..00000000 --- a/modules/nf-core/modules/untar/main.nf +++ /dev/null @@ -1,35 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process UNTAR { - tag "$archive" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } - - input: - path archive - - output: - path "$untar" , emit: untar - path "*.version.txt", emit: version - - script: - def software = getSoftwareName(task.process) - untar = archive.toString() - '.tar.gz' - """ - tar -xzvf $options.args $archive - echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//' > ${software}.version.txt - """ -} diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml deleted file mode 100644 index af4674f0..00000000 --- a/modules/nf-core/modules/untar/meta.yml +++ /dev/null @@ -1,27 +0,0 @@ -name: untar -description: Extract files. -keywords: - - untar - - uncompress -tools: - - untar: - description: | - Extract tar.gz files. - documentation: https://www.gnu.org/software/tar/manual/ -input: - - archive: - type: file - description: File to be untar - pattern: "*.{tar}.{gz}" -output: - - untar: - type: file - description: - pattern: "*.*" - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" -authors: - - "@joseespinosa" - - "@drpatelh" diff --git a/nextflow.config b/nextflow.config deleted file mode 100644 index edcb06c9..00000000 --- a/nextflow.config +++ /dev/null @@ -1,236 +0,0 @@ -/* -======================================================================================== - nf-core/rnavar Nextflow config file -======================================================================================== - Default config options for all compute environments ----------------------------------------------------------------------------------------- -*/ - -// Global default params, used in configs -params { - - // Pipeline options - - // Mandatory option - input = null // sample sheet - - // Genome and reference options - genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' - igenomes_ignore = false - save_reference = false - save_merged_fastq = false - gtf = null - - // Sequence read information - read_length = 150 // Required for STAR to build index and align reads - - // Alignment - aligner = 'star' // Only STAR is currently supported. - star_index = null // Provide STAR index if there is already a pre-defined one, or set to null to create one by the pipeline - star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR - seq_center = null - seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work - bam_csi_index = false - save_unaligned = false - save_align_intermeds = false - - //Known variant references - known_indels = null - known_indels_index = null - dbsnp_vcf = null - dbsnp_vcf_index = null - - // Variant calling - no_intervals = false - - // Skip steps - skip_intervallisttools = false - skip_variantfiltration = false - - // GATK intervallist parameters - scatter_count = 6 - - //GATK variant filter parameters - window = 35 - cluster = 3 - fs_filter = 30.0 - qd_filter = 2.0 - - // QC - skip_qc = false - - // MultiQC options - multiqc_config = null - multiqc_title = null - max_multiqc_email_size = '25.MB' - - // Boilerplate options - outdir = './results' - tracedir = "${params.outdir}/pipeline_info" - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - help = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes,modules' - enable_conda = false - singularity_pull_docker_container = false - - // Config options - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = [:] - config_profile_description = null - config_profile_contact = null - config_profile_url = null - config_profile_name = null - - // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' -} - -// Load base.config by default for all pipelines -includeConfig 'conf/base.config' - -// Load modules.config for DSL2 module specific options -includeConfig 'conf/modules.config' - -// Load nf-core custom profiles from different Institutions -try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} - -profiles { - debug { process.beforeScript = 'echo $HOSTNAME' } - conda { - params.enable_conda = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - docker { - docker.enabled = true - docker.userEmulation = true - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - singularity { - singularity.enabled = true - singularity.autoMounts = true - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - podman { - podman.enabled = true - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - shifter { - shifter.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - } - charliecloud { - charliecloud.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } -} - -// Export these variables to prevent local Python/R libraries from conflicting with those in the container -env { - PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" -} - -def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') -timeline { - enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" -} -report { - enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" -} -trace { - enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" -} -dag { - enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" -} - -manifest { - name = 'nf-core/rnavar' - author = 'Praveen Raj' - homePage = 'https://github.com/nf-core/rnavar' - description = 'Nextflow RNA-Seq analysis pipeline, part of the nf-core community.' - mainScript = 'main.nf' - nextflowVersion = '!>=21.04.0' - version = '3.1' -} - -// Function to ensure that resource requirements don't go beyond -// a maximum limit -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } - } -} diff --git a/nextflow_schema.json b/nextflow_schema.json deleted file mode 100644 index 7aa3e37e..00000000 --- a/nextflow_schema.json +++ /dev/null @@ -1,481 +0,0 @@ -{ - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/nextflow_schema.json", - "title": "nf-core/rnavar pipeline parameters", - "description": "Nextflow RNA-Seq analysis pipeline, part of the nf-core community.", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input" - ], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "mimetype": "text/csv", - "pattern": "^\\S+\\.csv$", - "schema": "assets/schema_input.json", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re//usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" - }, - "outdir": { - "type": "string", - "description": "Path to the output directory where the results will be saved.", - "default": "./results", - "fa_icon": "fas fa-folder-open" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - }, - "save_merged_fastq": { - "type": "string", - "description": "Save FastQ files after merging re-sequenced libraries in the results directory." - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." - }, - "fasta": { - "type": "string", - "format": "file-path", - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a STAR index available, this will be generated for you automatically. Combine with `--save_reference` to save STAR index for future runs.", - "fa_icon": "far fa-file-code" - }, - "fasta_dict": { - "type": "string", - "fa_icon": "fas fa-file", - "description": "Path to FASTA dictionary file.", - "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference." - }, - "fasta_fai": { - "type": "string", - "fa_icon": "fas fa-file", - "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference", - "description": "Path to FASTA reference index." - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true, - "help_text": "This parameter is to set the directory or the URL base for iGenomes references." - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - }, - "gtf": { - "type": "string", - "description": "Path to GTF annotation file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified." - }, - "gff": { - "type": "string", - "description": "Path to GFF3 annotation file.", - "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." - }, - "gene_bed": { - "type": "string", - "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." - }, - "read_length": { - "type": "number", - "default": 150, - "description": "Read length", - "help_text": "Specify the read length for the STAR aligner." - }, - "save_reference": { - "type": "string", - "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", - "description": "If generated by the pipeline, save the STAR index in the results directory." - }, - "known_indels": { - "type": "string", - "description": "Path to known indels VCF file" - }, - "known_indels_index": { - "type": "string", - "description": "Path to known indels index file" - }, - "dbsnp_vcf": { - "type": "string", - "description": "Path to dbSNP VCF file" - }, - "dbsnp_vcf_index": { - "type": "string", - "description": "Path to dbSNP VCF index file" - } - } - }, - "alignment_options": { - "title": "Alignment options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define parameters related to read alignment", - "properties": { - "aligner": { - "type": "string", - "default": "star", - "description": "Specifies the alignment algorithm to use. Currently available option is 'star'", - "help_text": "This parameter define which aligner is to be used for aligning the RNA reads to the reference genome. Currently only STAR aligner is supported. So use 'star' as the value for this option." - }, - "star_index": { - "type": "string", - "description": "Path to STAR index folder or compressed file (tar.gz)", - "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." - }, - "star_ignore_sjdbgtf": { - "type": "string", - "description": "Do not use GTF file during STAR index buidling step", - "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." - }, - "seq_center": { - "type": "string", - "description": "Sequencing center information to be added to read group of BAM files.", - "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " - }, - "seq_platform": { - "type": "string", - "default": "illumina", - "description": "Specify the sequencing platform used", - "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " - }, - "save_unaligned": { - "type": "string", - "description": "Where possible, save unaligned reads from aligner to the results directory.", - "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." - }, - "save_align_intermeds": { - "type": "string", - "description": "Save the intermediate BAM files from the alignment step.", - "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." - }, - "bam_csi_index": { - "type": "boolean", - "description": "Create a CSI index for BAM files instead of the traditional BAI index. This will be required for genomes with larger chromosome sizes." - } - } - }, - "pipeline_stage_options": { - "title": "Pipeline stage options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define parameters that control the stages in the pipeline", - "properties": { - "skip_intervallisttools": { - "type": "string", - "description": "Skip the process of preparing interval lists for the GATK variant calling step", - "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." - }, - "skip_variantfiltration": { - "type": "string", - "description": "Skip variant filtering of GATK", - "help_text": "Set this parameter if you don't want to filter any variants. " - }, - "skip_qc": { - "type": "string", - "description": "Skip QC reports", - "help_text": "This parameter disable all QC reports from recalibrated BAM file." - } - } - }, - "tool_parameters": { - "title": "Tool parameters", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define parameters of the tools used in the pipeline", - "properties": { - "scatter_count": { - "type": "integer", - "default": 6, - "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", - "help_text": "Set this parameter to decide the number of splits for the gene interval list file." - }, - "no_intervals": { - "type": "string", - "description": "Do not use gene interval file during variant calling", - "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", - "default": "False" - }, - "window": { - "type": "integer", - "default": 35, - "description": "The window size (in bases) in which to evaluate clustered SNPs.", - "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." - }, - "cluster": { - "type": "integer", - "default": 3, - "description": "The number of SNPs which make up a cluster. Must be at least 2.", - "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." - }, - "fs_filter": { - "type": "number", - "default": 30, - "description": "Value to be used for the FisherStrand (FS) filter", - "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" - }, - "qd_filter": { - "type": "number", - "default": 2, - "description": "Value to be used for the QualByDepth (QD) filter", - "help_text": "This parameter defines the value to use for the QualByDepth (QD) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 2.0" - } - } - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "hostnames": { - "type": "string", - "description": "Institutional configs hostname.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - }, - "singularity_pull_docker_container": { - "type": "boolean", - "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", - "hidden": true, - "fa_icon": "fas fa-toolbox", - "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." - } - } - } - }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/reference_genome_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - } - , - { - "$ref": "#/definitions/tool_parameters" - } - , - { - "$ref": "#/definitions/pipeline_stage_options" - } - , - { - "$ref": "#/definitions/alignment_options" - } - ], - "required": [ - "aligner", - "seq_platform" - ] -} \ No newline at end of file diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index c7bfae95..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -params.options = [:] - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channels(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channels(LinkedHashMap row) { - def meta = [:] - meta.id = row.sample - meta.sample = row.sample - meta.single_end = row.single_end.toBoolean() - meta.strandedness = row.strandedness - - def array = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - array = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return array -} diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf deleted file mode 100644 index f9a05d8d..00000000 --- a/subworkflows/local/prepare_genome.nf +++ /dev/null @@ -1,114 +0,0 @@ -// -// Uncompress and prepare reference genome files -// - -params.genome_options = [:] -params.gffread_options = [:] -params.index_options = [:] -params.star_index_options = [:] - -include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) -include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) -include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams(options: params.gffread_options) -include { GTF2BED } from '../../modules/local/gtf2bed' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) -include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) -include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_index_options) - - -workflow PREPARE_GENOME { - take: - prepare_tool_indices - - main: - - // - // Uncompress genome fasta file if required - // - if (params.fasta.endsWith('.gz')) { - ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzip - } else { - ch_fasta = file(params.fasta) - } - - // - // Uncompress GTF annotation file or create from GFF3 if required - // - ch_gffread_version = Channel.empty() - if (params.gtf) { - if (params.gtf.endsWith('.gz')) { - ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip - } else { - ch_gtf = file(params.gtf) - } - } else if (params.gff) { - if (params.gff.endsWith('.gz')) { - ch_gff = GUNZIP_GFF ( params.gff ).gunzip - } else { - ch_gff = file(params.gff) - } - ch_gtf = GFFREAD ( ch_gff ).gtf - ch_gffread_version = GFFREAD.out.version - } - - // - // Uncompress gene BED annotation file or create from GTF if required - // - if (params.gene_bed) { - if (params.gene_bed.endsWith('.gz')) { - ch_gene_bed = GUNZIP_GENE_BED ( params.gene_bed ).gunzip - } else { - ch_gene_bed = file(params.gene_bed) - } - } else { - ch_gene_bed = GTF2BED ( ch_gtf ) - } - - // Index the genome fasta - ch_fasta_fai = Channel.empty() - if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) - else ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai - - // Create dictionary file for the genome fasta - ch_fasta_dict = Channel.empty() - if (params.fasta_dict) ch_fasta_dict = file(params.fasta_dict) - else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict - - // - // Create chromosome sizes file - // - ch_chrom_sizes = GET_CHROM_SIZES ( ch_fasta ).sizes - - // - // Uncompress STAR index or generate from scratch if required - // - ch_star_index = Channel.empty() - ch_star_version = Channel.empty() - if ('star' in prepare_tool_indices) { - if (params.star_index) { - if (params.star_index.endsWith('.tar.gz')) { - ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar - } else { - ch_star_index = file(params.star_index) - } - } else { - ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index - ch_star_version = STAR_GENOMEGENERATE.out.version - } - } - - emit: - fasta = ch_fasta // path: genome.fasta - fai = ch_fasta_fai // path: genome.fasta.fai - dict = ch_fasta_dict // path: genome.fasta.dict - gtf = ch_gtf // path: genome.gtf - gene_bed = ch_gene_bed // path: gene.bed - chrom_sizes = ch_chrom_sizes // path: genome.sizes - star_index = ch_star_index // path: star/index/ - star_version = ch_star_version // path: *.version.txt - gffread_version = ch_gffread_version // path: *.version.txt -} diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf deleted file mode 100644 index 85fbd453..00000000 --- a/subworkflows/nf-core/align_star.nf +++ /dev/null @@ -1,50 +0,0 @@ -// -// Alignment with STAR -// - -params.align_options = [:] -params.samtools_index_options = [:] -params.samtools_sort_options = [:] -params.samtools_stats_options = [:] -params.seq_platform = [:] - -include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: params.seq_platform) -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) - -workflow ALIGN_STAR { - take: - reads // channel: [ val(meta), [ reads ] ] - index // channel: /path/to/star/index/ - gtf // channel: /path/to/genome.gtf - - main: - - // - // Map reads with STAR - // - STAR_ALIGN ( reads, index, gtf ) - - // - // Sort, index BAM file and run samtools stats, flagstat and idxstats - // - BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) - - emit: - orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] - log_final = STAR_ALIGN.out.log_final // channel: [ val(meta), log_final ] - log_out = STAR_ALIGN.out.log_out // channel: [ val(meta), log_out ] - log_progress = STAR_ALIGN.out.log_progress // channel: [ val(meta), log_progress ] - bam_sorted = STAR_ALIGN.out.bam_sorted // channel: [ val(meta), bam_sorted ] - bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] - fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] - tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] - star_version = STAR_ALIGN.out.version // path: *.version.txt - - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - csi = BAM_SORT_SAMTOOLS.out.csi // channel: [ val(meta), [ csi ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = BAM_SORT_SAMTOOLS.out.version // path: *.version.txt -} diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf deleted file mode 100644 index ba4d39ef..00000000 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ /dev/null @@ -1,45 +0,0 @@ -// -// Sort, index BAM file and run samtools stats, flagstat and idxstats -// - -params.sort_options = [:] -params.index_options = [:] -params.stats_options = [:] - -include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' addParams( options: params.sort_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) - -workflow BAM_SORT_SAMTOOLS { - take: - ch_bam // channel: [ val(meta), [ bam ] ] - - main: - SAMTOOLS_SORT ( ch_bam ) - SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) - - SAMTOOLS_SORT.out.bam - .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) - .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map { - meta, bam, bai, csi -> - if (bai) { - [ meta, bam, bai ] - } else { - [ meta, bam, csi ] - } - } - .set { ch_bam_bai } - - BAM_STATS_SAMTOOLS ( ch_bam_bai ) - - emit: - bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - version = SAMTOOLS_SORT.out.version // path: *.version.txt -} diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf deleted file mode 100644 index ca05241c..00000000 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ /dev/null @@ -1,25 +0,0 @@ -// -// Run SAMtools stats, flagstat and idxstats -// - -params.options = [:] - -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams( options: params.options ) -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' addParams( options: params.options ) -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' addParams( options: params.options ) - -workflow BAM_STATS_SAMTOOLS { - take: - ch_bam_bai // channel: [ val(meta), [ bam ], [bai/csi] ] - - main: - SAMTOOLS_STATS ( ch_bam_bai ) - SAMTOOLS_FLAGSTAT ( ch_bam_bai ) - SAMTOOLS_IDXSTATS ( ch_bam_bai ) - - emit: - stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - version = SAMTOOLS_STATS.out.version // path: *.version.txt -} diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf deleted file mode 100644 index 00a05d0d..00000000 --- a/subworkflows/nf-core/markduplicates.nf +++ /dev/null @@ -1,47 +0,0 @@ -// -// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats -// - -params.markduplicates_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] - -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams(options: params.samtools_stats_options) -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams(options: params.markduplicates_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) - -workflow MARKDUPLICATES { - take: - bam // channel: [ val(meta), [ bam ] ] - - main: - - PICARD_MARKDUPLICATES(bam) - - // - // Index BAM file and run samtools stats, flagstat and idxstats - // - SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) - - PICARD_MARKDUPLICATES.out.bam - .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) - .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map{meta, bam, bai, csi -> - if (bai) [meta, bam, bai] - else [meta, bam, csi]} - .set{ch_bam_bai} - - BAM_STATS_SAMTOOLS(ch_bam_bai) - - emit: - bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] - metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] - picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt - - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt -} diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf deleted file mode 100644 index 9cfccca5..00000000 --- a/subworkflows/nf-core/recalibrate.nf +++ /dev/null @@ -1,69 +0,0 @@ -/* -======================================================================================== - RECALIBRATE -======================================================================================== -*/ - -params.applybqsr_options = [:] -params.merge_bam_options = [:] -params.qualimap_bamqc_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] - -include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/local/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) -include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) - -workflow RECALIBRATE { - take: - skip_bamqc // boolean: true/false - skip_samtools // boolean: true/false - bam // channel: [mandatory] bam - dict // channel: [mandatory] dict - fai // channel: [mandatory] fai - fasta // channel: [mandatory] fasta - intervals // channel: [mandatory] intervals - - main: - - bam_recalibrated_index = Channel.empty() - bam_recalibrated = Channel.empty() - bam_reports = Channel.empty() - - bam.combine(intervals).map{ meta, bam, bai, recal, intervals -> - new_meta = meta.clone() - new_meta.id = meta.id - [new_meta, bam, bai, recal, intervals] - }.set{bam_intervals} - - APPLYBQSR(bam_intervals, fasta, fai, dict) - - // STEP 4.5: MERGING AND INDEXING THE RECALIBRATED BAM FILES - if (params.no_intervals) { - bam_recalibrated = APPLYBQSR.out.bam - } else { - APPLYBQSR.out.bam.map{ meta, bam -> - meta.id = meta.id - [meta, bam] - }.groupTuple().set{bam_recalibrated_interval} - - SAMTOOLS_MERGE(bam_recalibrated_interval) - bam_recalibrated = SAMTOOLS_MERGE.out.bam - - SAMTOOLS_INDEX(bam_recalibrated) - bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) - - samtools_stats = Channel.empty() - - if (!skip_samtools) { - SAMTOOLS_STATS(bam_recalibrated_index) - samtools_stats = SAMTOOLS_STATS.out.stats - } - bam_reports = samtools_stats - } - - emit: - bam = bam_recalibrated_index - qc = bam_reports -} diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf deleted file mode 100644 index 317944ef..00000000 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ /dev/null @@ -1,55 +0,0 @@ -// -// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats -// - -params.markduplicates_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] - -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) - -workflow MARK_DUPLICATES_PICARD { - take: - bam // channel: [ val(meta), [ bam ] ] - - main: - - // - // Picard MarkDuplicates - // - PICARD_MARKDUPLICATES ( bam ) - - // - // Index BAM file and run samtools stats, flagstat and idxstats - // - SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) - - PICARD_MARKDUPLICATES.out.bam - .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) - .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map { - meta, bam, bai, csi -> - if (bai) { - [ meta, bam, bai ] - } else { - [ meta, bam, csi ] - } - } - .set { ch_bam_bai } - - BAM_STATS_SAMTOOLS ( ch_bam_bai ) - - emit: - bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] - metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] - picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt - - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt -} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf deleted file mode 100644 index d82b01f9..00000000 --- a/workflows/rnavar.nf +++ /dev/null @@ -1,389 +0,0 @@ -/* -======================================================================================== - VALIDATE INPUTS -======================================================================================== -*/ - -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) - -// Validate input parameters -WorkflowRnavar.initialise(params, log) - -// Check input path parameters to see if they exist -def checkPathParamList = [ - params.input, - params.fasta, - params.gtf, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - params.known_indels, - params.known_indels_index, - params.star_index] - -for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} - -// Check mandatory parameters -if (params.input) ch_input = file(params.input) -else exit 1, 'Input samplesheet not specified!' - -if(!params.star_index and !params.gtf){ - exit 1, "GTF file is required to build a STAR reference index! Use option -gtf to provide a GTF file." -} - -/* -======================================================================================== - CONFIG FILES -======================================================================================== -*/ - -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() - -/* -======================================================================================== - IMPORT LOCAL MODULES/SUBWORKFLOWS -======================================================================================== -*/ - -// Don't overwrite global params.modules, create a copy instead and use that within the main script. -def modules = params.modules.clone() - -// -// MODULE: Local to the pipeline -// -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams(options: [publish_files : ['tsv':'']]) - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams(options: [:]) - -/* -======================================================================================== - IMPORT NF-CORE MODULES/SUBWORKFLOWS -======================================================================================== -*/ - -modules['multiqc'].args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams(options: modules['fastqc']) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams(options: modules['multiqc']) - -/* -======================================================================================== - RUN MAIN WORKFLOW -======================================================================================== -*/ - -// Check alignment parameters -def prepareToolIndices = [] -prepareToolIndices = params.aligner - -def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] -def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] - -if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false - -modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' - -if (params.save_align_intermeds) { - modules['samtools_sort_genome'].publish_files.put('bam','') - modules['samtools_index_genome'].publish_files.put('bai','') - modules['samtools_index_genome'].publish_files.put('csi','') -} - -if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false - -modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' -if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') -if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') - -modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) - -if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) -if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) -if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) -if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) - -include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) -include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) -include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) -include { GATK4_SPLITNCIGARREADS } from '../modules/local/gatk4/splitncigarreads/main' addParams(options: modules['gatk_splitncigar_options']) -include { GATK4_VARIANTFILTRATION } from '../modules/local/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) - -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( - genome_options: publish_genome_options, - index_options: publish_index_options, - star_index_options: modules['star_genomegenerate'] -) -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( - align_options: modules['star_align'], - samtools_index_options: modules['samtools_index_genome'], - samtools_sort_options: modules['samtools_sort_genome'], - samtools_stats_options: modules['samtools_index_genome'], - seq_platform: params.seq_platform -) -include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( - markduplicates_options: modules['picard_markduplicates'], - samtools_index_options: modules['picard_markduplicates_samtools'], - samtools_stats_options: modules['picard_markduplicates_samtools'] -) -include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( - applybqsr_options: modules['applybqsr'], - merge_bam_options: modules['merge_bam_recalibrate'], - samtools_index_options: modules['samtools_index_recalibrate'], - samtools_stats_options: modules['samtools_stats_recalibrate'] -) - -// Info required for completion email and summary -def multiqc_report = [] - -workflow RNAVAR { - - ch_software_versions = Channel.empty() - - PREPARE_GENOME(prepareToolIndices) - ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) - - ch_software_versions = Channel.empty() - ch_software_versions = ch_software_versions.mix(PREPARE_GENOME.out.gffread_version.ifEmpty(null)) - - INPUT_CHECK(ch_input) - .reads - .map{ meta, fastq -> - meta.id = meta.id.split('_')[0..-2].join('_') - [meta, fastq]} - .groupTuple(by: [0]) - .branch{ meta, fastq -> - single : fastq.size() == 1 - return [meta, fastq.flatten()] - multiple: fastq.size() > 1 - return [meta, fastq.flatten()]} - .set{ch_fastq} - //ch_software_versions = ch_software_versions.mix(INPUT_CHECK.out.version.first().ifEmpty(null)) - - ch_fastq.multiple.view() - - // MODULE: Concatenate FastQ files from same sample if required - CAT_FASTQ(ch_fastq.multiple) - .reads - .mix(ch_fastq.single) - .set{ch_cat_fastq} - //ch_software_versions = ch_software_versions.mix(CAT_FASTQ.out.version.first().ifEmpty(null)) - - // MODULE: Run FastQC - FASTQC(ch_cat_fastq) - ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - - - // PREPARE THE INTERVAL LIST FROM GTF FILE - ch_interval_list = Channel.empty() - GATK4_BEDTOINTERVALLIST(ch_genome_bed, PREPARE_GENOME.out.dict) - ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list - ch_software_versions = ch_software_versions.mix(GATK4_BEDTOINTERVALLIST.out.version.first().ifEmpty(null)) - - // - // SUBWORKFLOW: Alignment with STAR - // - ch_genome_bam = Channel.empty() - ch_genome_bam_index = Channel.empty() - ch_samtools_stats = Channel.empty() - ch_samtools_flagstat = Channel.empty() - ch_samtools_idxstats = Channel.empty() - ch_star_multiqc = Channel.empty() - ch_aligner_pca_multiqc = Channel.empty() - ch_aligner_clustering_multiqc = Channel.empty() - - if (params.aligner == 'star') { - ALIGN_STAR ( - ch_cat_fastq, - PREPARE_GENOME.out.star_index, - PREPARE_GENOME.out.gtf - ) - ch_genome_bam = ALIGN_STAR.out.bam - ch_genome_bam_index = ALIGN_STAR.out.bai - ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript - ch_samtools_stats = ALIGN_STAR.out.stats - ch_samtools_flagstat = ALIGN_STAR.out.flagstat - ch_samtools_idxstats = ALIGN_STAR.out.idxstats - ch_star_multiqc = ALIGN_STAR.out.log_final - if (params.bam_csi_index) ch_genome_bam_index = ALIGN_STAR.out.csi - ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.star_version.first().ifEmpty(null)) - ch_software_versions = ch_software_versions.mix(ALIGN_STAR.out.samtools_version.first().ifEmpty(null)) - - // SUBWORKFLOW: Mark duplicates with Picard - MARKDUPLICATES(ch_genome_bam) - ch_genome_bam = MARKDUPLICATES.out.bam - ch_genome_bam_index = MARKDUPLICATES.out.bai - ch_samtools_stats = MARKDUPLICATES.out.stats - ch_samtools_flagstat = MARKDUPLICATES.out.flagstat - ch_samtools_idxstats = MARKDUPLICATES.out.idxstats - ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics - if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi - ch_software_versions = ch_software_versions.mix(MARKDUPLICATES.out.picard_version.first().ifEmpty(null)) - - // MODULE: SplitNCigarReads from GATK4 - // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). - bam_splitncigar = Channel.empty() - GATK4_SPLITNCIGARREADS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) - bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam - ch_software_versions = ch_software_versions.mix(GATK4_SPLITNCIGARREADS.out.version.first().ifEmpty(null)) - - ch_bqsr_table = Channel.empty() - - // MODULE: BaseRecalibrator from GATK4 - known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() - known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() - - ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() - - GATK4_BASERECALIBRATOR( - bam_splitncigar, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - ch_interval_list_baserecalibrator, - known_sites, - known_sites_tbi - ) - ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table - - - // MODULE: ApplyBaseRecalibrator from GATK4 - bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) - bam_recalibrated = Channel.empty() - bam_recalibrated_qc = Channel.empty() - - ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() - - RECALIBRATE( - ('bamqc' in params.skip_qc), - ('samtools' in params.skip_qc), - bam_applybqsr, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.fasta, - ch_interval_list_applybqsr - ) - - bam_recalibrated = RECALIBRATE.out.bam - bam_recalibrated_qc = RECALIBRATE.out.qc - - // MODULE: IntervalListTools from GATK4 - ch_interval_list_split = Channel.empty() - - if (!params.skip_intervallisttools) { - GATK4_INTERVALLISTTOOLS(ch_interval_list) - ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() - } - else ch_interval_list_split = ch_interval_list - - - // MODULE: HaplotypeCaller from GATK4 - interval_flag = false - haplotypecaller_vcf = Channel.empty() - - haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) - .map{ meta, bam, bai, interval_list -> - new_meta = meta.clone() - new_meta.id = meta.id + "_" + interval_list.baseName - [new_meta, bam, bai, interval_list]} - - GATK4_HAPLOTYPECALLER( - haplotypecaller_interval_bam, - params.dbsnp_vcf, - params.dbsnp_vcf_index, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - interval_flag - ) - - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf - .map{ meta,vcf -> - meta.id = meta.sample - [meta, vcf]} - .groupTuple() - - use_ref_dict = true - GATK4_MERGEVCFS( - haplotypecaller_raw, - PREPARE_GENOME.out.dict, - use_ref_dict - ) - - haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf - - // MODULE: VariantFiltration from GATK4 - - if (!params.skip_variantfiltration) { - - GATK4_VARIANTFILTRATION( - haplotypecaller_vcf, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict - ) - - filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf - } - - } - - // - // MODULE: Pipeline reporting - // - ch_software_versions - .map{it -> if (it) [it.baseName, it]} - .groupTuple() - .map{it[1][0]} - .flatten() - .collect() - .set{ch_software_versions} - - GET_SOFTWARE_VERSIONS(ch_software_versions.map{it}.collect()) - - // - // MODULE: MultiQC - // - workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC (ch_multiqc_files.collect()) - multiqc_report = MULTIQC.out.report.toList() - ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) -} - -/* -======================================================================================== - COMPLETION EMAIL AND SUMMARY -======================================================================================== -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.summary(workflow, params, log) -} - -/* -======================================================================================== - THE END -======================================================================================== -*/ From cfd9998d3b1f2c45311af3d2649eb6817105bf08 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 11 Nov 2021 17:25:34 +0100 Subject: [PATCH 042/235] cleaned --- .gitattributes | 2 + CHANGELOG.md | 16 + CITATIONS.md | 32 ++ CODE_OF_CONDUCT.md | 111 ++++ LICENSE | 21 + README.md | 90 +++ assets/email_template.html | 53 ++ assets/email_template.txt | 40 ++ assets/multiqc_config.yaml | 11 + assets/nf-core-rnavar_logo.png | Bin 0 -> 16469 bytes assets/samplesheet.csv | 3 + assets/schema_input.json | 39 ++ assets/sendmail_template.txt | 53 ++ bin/check_samplesheet.py | 146 +++++ conf/base.config | 60 ++ conf/igenomes.config | 432 +++++++++++++++ conf/modules.config | 32 ++ conf/test.config | 29 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modules/nf-core/modules/fastqc/functions.nf | 78 +++ modules/nf-core/modules/fastqc/main.nf | 54 ++ modules/nf-core/modules/fastqc/meta.yml | 52 ++ modules/nf-core/modules/multiqc/functions.nf | 78 +++ modules/nf-core/modules/multiqc/main.nf | 38 ++ modules/nf-core/modules/multiqc/meta.yml | 40 ++ nextflow.config | 197 +++++++ nextflow_schema.json | 285 ++++++++++ subworkflows/local/input_check.nf | 44 ++ workflows/rnavar.nf | 129 +++++ 52 files changed, 4055 insertions(+) create mode 100644 CHANGELOG.md create mode 100644 CITATIONS.md create mode 100644 CODE_OF_CONDUCT.md create mode 100644 LICENSE create mode 100644 README.md create mode 100644 assets/email_template.html create mode 100644 assets/email_template.txt create mode 100644 assets/multiqc_config.yaml create mode 100644 assets/nf-core-rnavar_logo.png create mode 100644 assets/samplesheet.csv create mode 100644 assets/schema_input.json create mode 100644 assets/sendmail_template.txt create mode 100755 bin/check_samplesheet.py create mode 100644 conf/base.config create mode 100644 conf/igenomes.config create mode 100644 conf/modules.config create mode 100644 conf/test.config create mode 100644 conf/test_full.config create mode 100644 docs/README.md create mode 100755 docs/images/mqc_fastqc_adapter.png create mode 100755 docs/images/mqc_fastqc_counts.png create mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 docs/images/nf-core-rnavar_logo.png create mode 100644 docs/output.md create mode 100644 docs/usage.md create mode 100755 lib/NfcoreSchema.groovy create mode 100755 lib/NfcoreTemplate.groovy create mode 100755 lib/Utils.groovy create mode 100755 lib/WorkflowMain.groovy create mode 100755 lib/WorkflowRnavar.groovy create mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 main.nf create mode 100644 modules.json create mode 100644 modules/local/functions.nf create mode 100644 modules/local/get_chrom_sizes.nf create mode 100644 modules/local/gtf2bed.nf create mode 100644 modules/local/gtf_gene_filter.nf create mode 100644 modules/local/samplesheet_check.nf create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/main.nf create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml create mode 100644 modules/nf-core/modules/fastqc/functions.nf create mode 100644 modules/nf-core/modules/fastqc/main.nf create mode 100644 modules/nf-core/modules/fastqc/meta.yml create mode 100644 modules/nf-core/modules/multiqc/functions.nf create mode 100644 modules/nf-core/modules/multiqc/main.nf create mode 100644 modules/nf-core/modules/multiqc/meta.yml create mode 100644 nextflow.config create mode 100644 nextflow_schema.json create mode 100644 subworkflows/local/input_check.nf create mode 100644 workflows/rnavar.nf diff --git a/.gitattributes b/.gitattributes index 7fe55006..050bb120 100644 --- a/.gitattributes +++ b/.gitattributes @@ -1 +1,3 @@ *.config linguist-language=nextflow +modules/nf-core/** linguist-generated +subworkflows/nf-core/** linguist-generated diff --git a/CHANGELOG.md b/CHANGELOG.md new file mode 100644 index 00000000..cb967546 --- /dev/null +++ b/CHANGELOG.md @@ -0,0 +1,16 @@ +# nf-core/rnavar: Changelog + +The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) +and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). + +## v1.0dev - [date] + +Initial release of nf-core/rnavar, created with the [nf-core](https://nf-co.re/) template. + +### `Added` + +### `Fixed` + +### `Dependencies` + +### `Deprecated` diff --git a/CITATIONS.md b/CITATIONS.md new file mode 100644 index 00000000..e040b48b --- /dev/null +++ b/CITATIONS.md @@ -0,0 +1,32 @@ +# nf-core/rnavar: Citations + +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) + +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. + +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) + +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. + +## Pipeline tools + +* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + +* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + +## Software packaging/containerisation tools + +* [Anaconda](https://anaconda.com) + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. + +* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. + +* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. + +* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + +* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md new file mode 100644 index 00000000..f4fd052f --- /dev/null +++ b/CODE_OF_CONDUCT.md @@ -0,0 +1,111 @@ +# Code of Conduct at nf-core (v1.0) + +## Our Pledge + +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: + +- Age +- Body size +- Familial status +- Gender identity and expression +- Geographical location +- Level of experience +- Nationality and national origins +- Native language +- Physical and neurological ability +- Race or ethnicity +- Religion +- Sexual identity and orientation +- Socioeconomic status + +Please note that the list above is alphabetised and is therefore not ranked in any order of preference or importance. + +## Preamble + +> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. + +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. + +nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. + +We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. + +Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. + +We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. + +Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re + +## Our Responsibilities + +The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. + +The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. + +Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. + +## When are where does this Code of Conduct apply? + +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: + +- Communicating with an official project email address. +- Communicating with community members within the nf-core Slack channel. +- Participating in hackathons organised by nf-core (both online and in-person events). +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Representing nf-core on social media. This includes both official and personal accounts. + +## nf-core cares 😊 + +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): + +- Ask for consent before sharing another community member’s personal information (including photographs) on social media. +- Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. +- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) +- Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) +- Focus on what is best for the team and the community. (When in doubt, ask) +- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Take breaks when you feel like you need them. +- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) + +## nf-core frowns on 😕 + +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. + +- Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. +- “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. +- Spamming or trolling of individuals on social media. +- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. + +### Online Trolling + +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. + +All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. + +## Procedures for Reporting CoC violations + +If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. + +You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). + +Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. + +All reports will be handled with utmost discretion and confidentially. + +## Attribution and Acknowledgements + +- The [Contributor Covenant, version 1.4](http://contributor-covenant.org/version/1/4) +- The [OpenCon 2017 Code of Conduct](http://www.opencon2017.org/code_of_conduct) (CC BY 4.0 OpenCon organisers, SPARC and Right to Research Coalition) +- The [eLife innovation sprint 2020 Code of Conduct](https://sprint.elifesciences.org/code-of-conduct/) +- The [Mozilla Community Participation Guidelines v3.1](https://www.mozilla.org/en-US/about/governance/policies/participation/) (version 3.1, CC BY-SA 3.0 Mozilla) + +## Changelog + +### v1.0 - March 12th, 2021 + +- Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/LICENSE b/LICENSE new file mode 100644 index 00000000..2a63dfa9 --- /dev/null +++ b/LICENSE @@ -0,0 +1,21 @@ +MIT License + +Copyright (c) @praveenraj2018 + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in all +copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +SOFTWARE. diff --git a/README.md b/README.md new file mode 100644 index 00000000..8793c99c --- /dev/null +++ b/README.md @@ -0,0 +1,90 @@ +# ![nf-core/rnavar](docs/images/nf-core-rnavar_logo.png) + +[![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) +[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) +[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) +[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) +[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) + +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnavar-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnavar) +[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core) +[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) + +## Introduction + + +**nf-core/rnavar** is a bioinformatics best-practice analysis pipeline for GATK4 RNA variant calling pipeline. + +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! + + +On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnavar/results). + +## Pipeline summary + + + +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) + +## Quick Start + +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`) + +2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ + +3. Download the pipeline and test it on a minimal dataset with a single command: + + ```console + nextflow run nf-core/rnavar -profile test, + ``` + + > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs. + > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. + +4. Start running your own analysis! + + + + ```console + nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh37 + ``` + +## Documentation + +The nf-core/rnavar pipeline comes with documentation about the pipeline [usage](https://nf-co.re/rnavar/usage), [parameters](https://nf-co.re/rnavar/parameters) and [output](https://nf-co.re/rnavar/output). + +## Credits + +nf-core/rnavar was originally written by @praveenraj2018. + +We thank the following people for their extensive assistance in the development of this pipeline: + + + +## Contributions and Support + +If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). + +For further information or help, don't hesitate to get in touch on the [Slack `#rnavar` channel](https://nfcore.slack.com/channels/rnavar) (you can join with [this invite](https://nf-co.re/join/slack)). + +## Citations + + + + + +An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. + +You can cite the `nf-core` publication as follows: + +> **The nf-core framework for community-curated bioinformatics pipelines.** +> +> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. +> +> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). diff --git a/assets/email_template.html b/assets/email_template.html new file mode 100644 index 00000000..e8bda58a --- /dev/null +++ b/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/rnavar Pipeline Report + + +

+ + + diff --git a/assets/email_template.txt b/assets/email_template.txt new file mode 100644 index 00000000..2c43ec61 --- /dev/null +++ b/assets/email_template.txt @@ -0,0 +1,40 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/rnavar v${version} +---------------------------------------------------- + +Run Name: $runName + +<% if (success){ + out << "## nf-core/rnavar execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/rnavar execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/rnavar +https://github.com/nf-core/rnavar diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml new file mode 100644 index 00000000..917528e8 --- /dev/null +++ b/assets/multiqc_config.yaml @@ -0,0 +1,11 @@ +report_comment: > + This report has been generated by the nf-core/rnavar + analysis pipeline. For information about how to interpret these results, please see the + documentation. +report_section_order: + software_versions: + order: -1000 + nf-core-rnavar-summary: + order: -1001 + +export_plots: true diff --git a/assets/nf-core-rnavar_logo.png b/assets/nf-core-rnavar_logo.png new file mode 100644 index 0000000000000000000000000000000000000000..482af6ee764c26783cee9e3e82ab3a7c5a892415 GIT binary patch literal 16469 zcmX|J1yogAn7))C-5`y0hk%H5N=b-xi*%PXNDC55cXvy7w;9JmfeA>BX#~yxPM0cvu^l8-j4~9WnmGq7+vP;}>_IIHMB!NhTePo|Syj zz4+^4+>qwhklR?eXZbSZB?3eg3F4!STw1@CXi-{!nft98vTDmqf-Z&ZTTNZj!9A_* zFvLAAm#Dc%O@bcOx%y7nmQ|?kzl*mLCC&sfp})NL*Jl%5O6~dY>Ix*)B; zAh&p9C;;4nZdl1W-1EXAf^>2D^ldV}OnCFZ*S0_%^XKKu-pdV#gbrdAy zF8d1J_q~34wBgfH<8jaVc6vdPn2`~#+r?f`)pyjL3%>g*V>Cz2l1>h*`PyE@vsQ6s z8JXawOVWA=EmFw8Z}c3=J3T$Vb@(G%G^)Q6N$GMJqxK_yL6Mz)1G)JAPc6hJI2dW+ z>X?`RbV5a3Qi8j%+rRV1W&D5N6ym2FK}X(199g=F@fnyOz46GM&~&Lvqog~fq-^KwBfE=X!%N%#yg8eBE3@e0Q%~=gBIcI 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+Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py new file mode 100755 index 00000000..7634b236 --- /dev/null +++ b/bin/check_samplesheet.py @@ -0,0 +1,146 @@ +#!/usr/bin/env python + +# TODO nf-core: Update the script to check the samplesheet +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + +import os +import sys +import errno +import argparse + + +def parse_args(args=None): + Description = "Reformat nf-core/rnavar samplesheet file and check its contents." + Epilog = "Example usage: python check_samplesheet.py " + + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") + return parser.parse_args(args) + + +def make_dir(path): + if len(path) > 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise exception + + +def print_error(error, context="Line", context_str=""): + error_str = "ERROR: Please check samplesheet -> {}".format(error) + if context != "" and context_str != "": + error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( + error, context.strip(), context_str.strip() + ) + print(error_str) + sys.exit(1) + + +# TODO nf-core: Update the check_samplesheet function +def check_samplesheet(file_in, file_out): + """ + This function checks that the samplesheet follows the following structure: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + For an example see: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + """ + + sample_mapping_dict = {} + with open(file_in, "r") as fin: + + ## Check header + MIN_COLS = 2 + # TODO nf-core: Update the column names for the input samplesheet + HEADER = ["sample", "fastq_1", "fastq_2"] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[: len(HEADER)] != HEADER: + print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) + sys.exit(1) + + ## Check sample entries + for line in fin: + lspl = [x.strip().strip('"') for x in line.strip().split(",")] + + # Check valid number of columns per row + if len(lspl) < len(HEADER): + print_error( + "Invalid number of columns (minimum = {})!".format(len(HEADER)), + "Line", + line, + ) + num_cols = len([x for x in lspl if x]) + if num_cols < MIN_COLS: + print_error( + "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), + "Line", + line, + ) + + ## Check sample name entries + sample, fastq_1, fastq_2 = lspl[: len(HEADER)] + sample = sample.replace(" ", "_") + if not sample: + print_error("Sample entry has not been specified!", "Line", line) + + ## Check FastQ file extension + for fastq in [fastq_1, fastq_2]: + if fastq: + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", "Line", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error( + "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "Line", + line, + ) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2] + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ["0", fastq_1, fastq_2] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ["1", fastq_1, fastq_2] + else: + print_error("Invalid combination of columns provided!", "Line", line) + + ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] } + if sample not in sample_mapping_dict: + sample_mapping_dict[sample] = [sample_info] + else: + if sample_info in sample_mapping_dict[sample]: + print_error("Samplesheet contains duplicate rows!", "Line", line) + else: + sample_mapping_dict[sample].append(sample_info) + + ## Write validated samplesheet with appropriate columns + if len(sample_mapping_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + with open(file_out, "w") as fout: + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") + for sample in sorted(sample_mapping_dict.keys()): + + ## Check that multiple runs of the same sample are of the same datatype + if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): + print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) + + for idx, val in enumerate(sample_mapping_dict[sample]): + fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") + else: + print_error("No entries to process!", "Samplesheet: {}".format(file_in)) + + +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN, args.FILE_OUT) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/conf/base.config b/conf/base.config new file mode 100644 index 00000000..1e805048 --- /dev/null +++ b/conf/base.config @@ -0,0 +1,60 @@ +/* +======================================================================================== + nf-core/rnavar Nextflow base config file +======================================================================================== + A 'blank slate' config file, appropriate for general use on most high performance + compute environments. Assumes that all software is installed and available on + the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. +---------------------------------------------------------------------------------------- +*/ + +process { + + // TODO nf-core: Check the defaults for all processes + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } + + errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + maxRetries = 1 + maxErrors = '-1' + + // Process-specific resource requirements + // NOTE - Please try and re-use the labels below as much as possible. + // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. + // If possible, it would be nice to keep the same label naming convention when + // adding in your local modules too. + // TODO nf-core: Customise requirements for specific processes. + // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withLabel:process_high_memory { + memory = { check_max( 200.GB * task.attempt, 'memory' ) } + } + withLabel:error_ignore { + errorStrategy = 'ignore' + } + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 + } + withName:CUSTOM_DUMPSOFTWAREVERSIONS { + cache = false + } +} diff --git a/conf/igenomes.config b/conf/igenomes.config new file mode 100644 index 00000000..855948de --- /dev/null +++ b/conf/igenomes.config @@ -0,0 +1,432 @@ +/* +======================================================================================== + Nextflow config file for iGenomes paths +======================================================================================== + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ + +params { + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_5.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } + } +} diff --git a/conf/modules.config b/conf/modules.config new file mode 100644 index 00000000..0b1bfdec --- /dev/null +++ b/conf/modules.config @@ -0,0 +1,32 @@ +/* +======================================================================================== + Config file for defining DSL2 per module options +======================================================================================== + Available keys to override module options: + args = Additional arguments appended to command in module. + args2 = Second set of arguments appended to command in module (multi-tool modules). + args3 = Third set of arguments appended to command in module (multi-tool modules). + publish_dir = Directory to publish results. + publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path + If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path + If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" + is appended as a directory to "publish_dir" path + If publish_by_meta = false / null - No directories are appended to "publish_dir" path + publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + The value of "directory" is appended to the standard "publish_dir" path as defined above. + If publish_files = null (unspecified) - All files are published. + If publish_files = false - No files are published. + suffix = File name suffix for output files. +---------------------------------------------------------------------------------------- +*/ + +params { + modules { + 'fastqc' { + args = "--quiet" + } + 'multiqc' { + args = "" + } + } +} diff --git a/conf/test.config b/conf/test.config new file mode 100644 index 00000000..c69c8094 --- /dev/null +++ b/conf/test.config @@ -0,0 +1,29 @@ +/* +======================================================================================== + Nextflow config file for running minimal tests +======================================================================================== + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/rnavar -profile test, + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + + // Genome references + genome = 'R64-1-1' +} diff --git a/conf/test_full.config b/conf/test_full.config new file mode 100644 index 00000000..78d70ce3 --- /dev/null +++ b/conf/test_full.config @@ -0,0 +1,24 @@ +/* +======================================================================================== + Nextflow config file for running full-size tests +======================================================================================== + Defines input files and everything required to run a full size pipeline test. + + Use as follows: + nextflow run nf-core/rnavar -profile test_full, + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' + + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + + // Genome references + genome = 'R64-1-1' +} diff --git a/docs/README.md b/docs/README.md new file mode 100644 index 00000000..e5f538d3 --- /dev/null +++ b/docs/README.md @@ -0,0 +1,10 @@ +# nf-core/rnavar: Documentation + +The nf-core/rnavar documentation is split into the following pages: + +* [Usage](usage.md) + * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. +* [Output](output.md) + * An overview of the different results produced by the pipeline and how to interpret them. + +You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png new file mode 100755 index 0000000000000000000000000000000000000000..361d0e47acfb424dea1f326590d1eb2f6dfa26b5 GIT binary patch literal 23458 zcmeFZ2UJtryD!S#x<#o93es(Ww4k)maRbte0-+a?-g^xY-3myTE`8G_KvA54)F1tn})nJ5u%TA4Y;^!^{48eL_}p#q-Umo0M|F1 z74+PQh^X8N|9_jcWbq~ 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za~#MIdK%`Cg9$P$ruqLrvL<}k*r=UwNPP;>%r;fAeVVBchD@e-Un-oiE6bnGyJeR>DiuF;o=P K<%@3{`~4rFG(!*o literal 0 HcmV?d00001 diff --git a/docs/output.md b/docs/output.md new file mode 100644 index 00000000..f18b6fa6 --- /dev/null +++ b/docs/output.md @@ -0,0 +1,68 @@ +# nf-core/rnavar: Output + +## Introduction + +This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. + +The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. + + + +## Pipeline overview + +The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: + +* [FastQC](#fastqc) - Raw read QC +* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + +### FastQC + +
+Output files + +* `fastqc/` + * `*_fastqc.html`: FastQC report containing quality metrics. + * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. + +
+ +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) + +![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) + +![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) + +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. + +### MultiQC + +
+Output files + +* `multiqc/` + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats. + +
+ +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +### Pipeline information + +
+Output files + +* `pipeline_info/` + * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. + * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + +
+ +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. diff --git a/docs/usage.md b/docs/usage.md new file mode 100644 index 00000000..00081b8b --- /dev/null +++ b/docs/usage.md @@ -0,0 +1,284 @@ +# nf-core/rnavar: Usage + +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/rnavar/usage](https://nf-co.re/rnavar/usage) + +> _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ + +## Introduction + + + +## Samplesheet input + +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. + +```console +--input '[path to samplesheet file]' +``` + +### Multiple runs of the same sample + +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +``` + +### Full samplesheet + +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. + +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +``` + +| Column | Description | +|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | + +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. + +## Running the pipeline + +The typical command for running the pipeline is as follows: + +```console +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh37 -profile docker +``` + +This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. + +Note that the pipeline will create the following files in your working directory: + +```console +work # Directory containing the nextflow working files +results # Finished results (configurable, see below) +.nextflow_log # Log file from Nextflow +# Other nextflow hidden files, eg. history of pipeline runs and old logs. +``` + +### Updating the pipeline + +When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: + +```console +nextflow pull nf-core/rnavar +``` + +### Reproducibility + +It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. + +First, go to the [nf-core/rnavar releases page](https://github.com/nf-core/rnavar/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. + +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. + +## Core Nextflow arguments + +> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). + +### `-profile` + +Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. + +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/). + +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. + +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). + +Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! +They are loaded in sequence, so later profiles can overwrite earlier profiles. + +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. + +* `docker` + * A generic configuration profile to be used with [Docker](https://docker.com/) +* `singularity` + * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) +* `podman` + * A generic configuration profile to be used with [Podman](https://podman.io/) +* `shifter` + * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) +* `charliecloud` + * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) +* `conda` + * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. +* `test` + * A profile with a complete configuration for automated testing + * Includes links to test data so needs no other parameters + +### `-resume` + +Specify this when restarting a pipeline. Nextflow will used cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. + +You can also supply a run name to resume a specific run: `-resume [run-name]`. Use the `nextflow log` command to show previous run names. + +### `-c` + +Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. + +## Custom configuration + +### Resource requests + +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. + +For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: + +```console +[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) +Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' + +Caused by: + Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) + +Command executed: + STAR \ + --genomeDir star \ + --readFilesIn WT_REP1_trimmed.fq.gz \ + --runThreadN 2 \ + --outFileNamePrefix WT_REP1. \ + + +Command exit status: + 137 + +Command output: + (empty) + +Command error: + .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. +Work dir: + /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb + +Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` +``` + +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. + +```nextflow +process { + withName: STAR_ALIGN { + memory = 100.GB + } +} +``` + +> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. + +### Tool-specific options + +For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes. + +The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: + +* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. + +* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. + +The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. + +When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. + +As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. + +Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. + +As you will see in the example below, we have: + +* appended `--outFilterMismatchNmax 16` to the default `args` used by the module. +* changed the default `publish_dir` value to where the files will eventually be published in the main results directory. +* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`. + +```nextflow +params { + modules { + 'star_align' { + args = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16" + publish_dir = "my_star_directory" + publish_files = ['out':'log', 'tab':'log', 'bam':''] + } + } +} +``` + +### Updating containers + +The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. + +1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) +2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) +3. Create the custom config accordingly: + + * For Docker: + + ```nextflow + process { + withName: PANGOLIN { + container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Singularity: + + ```nextflow + process { + withName: PANGOLIN { + container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Conda: + + ```nextflow + process { + withName: PANGOLIN { + conda = 'bioconda::pangolin=3.0.5' + } + } + ``` + +> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. + +### nf-core/configs + +In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. + +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. + +If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). + +## Running in the background + +Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. + +The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. + +Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. +Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). + +## Nextflow memory requirements + +In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. +We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): + +```console +NXF_OPTS='-Xms1g -Xmx4g' +``` diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy new file mode 100755 index 00000000..dcb39c83 --- /dev/null +++ b/lib/NfcoreSchema.groovy @@ -0,0 +1,516 @@ +// +// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. +// + +import org.everit.json.schema.Schema +import org.everit.json.schema.loader.SchemaLoader +import org.everit.json.schema.ValidationException +import org.json.JSONObject +import org.json.JSONTokener +import org.json.JSONArray +import groovy.json.JsonSlurper +import groovy.json.JsonBuilder + +class NfcoreSchema { + + // + // Resolve Schema path relative to main workflow directory + // + public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { + return "${workflow.projectDir}/${schema_filename}" + } + + // + // Function to loop over all parameters defined in schema and check + // whether the given parameters adhere to the specifications + // + /* groovylint-disable-next-line UnusedPrivateMethodParameter */ + public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { + def has_error = false + //=====================================================================// + // Check for nextflow core params and unexpected params + def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text + def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') + def nf_params = [ + // Options for base `nextflow` command + 'bg', + 'c', + 'C', + 'config', + 'd', + 'D', + 'dockerize', + 'h', + 'log', + 'q', + 'quiet', + 'syslog', + 'v', + 'version', + + // Options for `nextflow run` command + 'ansi', + 'ansi-log', + 'bg', + 'bucket-dir', + 'c', + 'cache', + 'config', + 'dsl2', + 'dump-channels', + 'dump-hashes', + 'E', + 'entry', + 'latest', + 'lib', + 'main-script', + 'N', + 'name', + 'offline', + 'params-file', + 'pi', + 'plugins', + 'poll-interval', + 'pool-size', + 'profile', + 'ps', + 'qs', + 'queue-size', + 'r', + 'resume', + 'revision', + 'stdin', + 'stub', + 'stub-run', + 'test', + 'w', + 'with-charliecloud', + 'with-conda', + 'with-dag', + 'with-docker', + 'with-mpi', + 'with-notification', + 'with-podman', + 'with-report', + 'with-singularity', + 'with-timeline', + 'with-tower', + 'with-trace', + 'with-weblog', + 'without-docker', + 'without-podman', + 'work-dir' + ] + def unexpectedParams = [] + + // Collect expected parameters from the schema + def expectedParams = [] + for (group in schemaParams) { + for (p in group.value['properties']) { + expectedParams.push(p.key) + } + } + + for (specifiedParam in params.keySet()) { + // nextflow params + if (nf_params.contains(specifiedParam)) { + log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" + has_error = true + } + // unexpected params + def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' + def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } + def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { + // Temporarily remove camelCase/camel-case params #1035 + def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} + if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ + unexpectedParams.push(specifiedParam) + } + } + } + + //=====================================================================// + // Validate parameters against the schema + InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() + JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) + + // Remove anything that's in params.schema_ignore_params + raw_schema = removeIgnoredParams(raw_schema, params) + + Schema schema = SchemaLoader.load(raw_schema) + + // Clean the parameters + def cleanedParams = cleanParameters(params) + + // Convert to JSONObject + def jsonParams = new JsonBuilder(cleanedParams) + JSONObject params_json = new JSONObject(jsonParams.toString()) + + // Validate + try { + schema.validate(params_json) + } catch (ValidationException e) { + println '' + log.error 'ERROR: Validation of pipeline parameters failed!' + JSONObject exceptionJSON = e.toJSON() + printExceptions(exceptionJSON, params_json, log) + println '' + has_error = true + } + + // Check for unexpected parameters + if (unexpectedParams.size() > 0) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + println '' + def warn_msg = 'Found unexpected parameters:' + for (unexpectedParam in unexpectedParams) { + warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" + } + log.warn warn_msg + log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" + println '' + } + + if (has_error) { + System.exit(1) + } + } + + // + // Beautify parameters for --help + // + public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + Integer num_hidden = 0 + String output = '' + output += 'Typical pipeline command:\n\n' + output += " ${colors.cyan}${command}${colors.reset}\n\n" + Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + Integer max_chars = paramsMaxChars(params_map) + 1 + Integer desc_indent = max_chars + 14 + Integer dec_linewidth = 160 - desc_indent + for (group in params_map.keySet()) { + Integer num_params = 0 + String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (group_params.get(param).hidden && !params.show_hidden_params) { + num_hidden += 1 + continue; + } + def type = '[' + group_params.get(param).type + ']' + def description = group_params.get(param).description + def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : '' + def description_default = description + colors.dim + defaultValue + colors.reset + // Wrap long description texts + // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap + if (description_default.length() > dec_linewidth){ + List olines = [] + String oline = "" // " " * indent + description_default.split(" ").each() { wrd -> + if ((oline.size() + wrd.size()) <= dec_linewidth) { + oline += wrd + " " + } else { + olines += oline + oline = wrd + " " + } + } + olines += oline + description_default = olines.join("\n" + " " * desc_indent) + } + group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' + num_params += 1 + } + group_output += '\n' + if (num_params > 0){ + output += group_output + } + } + if (num_hidden > 0){ + output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset + } + output += NfcoreTemplate.dashedLine(params.monochrome_logs) + return output + } + + // + // Groovy Map summarising parameters/workflow options used by the pipeline + // + public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = workflow.containerEngine + } + if (workflow.container) { + workflow_summary['container'] = workflow.container + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value != null) { + if (param_type == 'string') { + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir', '') + sub_string = sub_string.replace('\${projectDir}', '') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir', '') + sub_string = sub_string.replace('\${params.outdir}', '') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } + + // We have a default in the schema, and this isn't it + if (schema_value != null && params_value != schema_value) { + sub_params.put(param, params_value) + } + // No default in the schema, and this isn't empty + else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { + sub_params.put(param, params_value) + } + } + } + params_summary.put(group, sub_params) + } + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + // + // Beautify parameters for summary and return as string + // + public static String paramsSummaryLog(workflow, params) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + String output = '' + def params_map = paramsSummaryMap(workflow, params) + def max_chars = paramsMaxChars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += colors.bold + group + colors.reset + '\n' + for (param in group_params.keySet()) { + output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' + } + output += '\n' + } + } + output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" + output += NfcoreTemplate.dashedLine(params.monochrome_logs) + return output + } + + // + // Loop over nested exceptions and print the causingException + // + private static void printExceptions(ex_json, params_json, log) { + def causingExceptions = ex_json['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (ex_json['pointerToViolation'] == '#') { + log.error "* ${ex_json['message']}" + } + // Error with specific param + else { + def param = ex_json['pointerToViolation'] - ~/^#\// + def param_val = params_json[param].toString() + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, params_json, log) + } + } + + // + // Remove an element from a JSONArray + // + private static JSONArray removeElement(json_array, element) { + def list = [] + int len = json_array.length() + for (int i=0;i + if(raw_schema.keySet().contains('definitions')){ + raw_schema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { + raw_schema.get("properties").remove(ignore_param) + } + if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { + def cleaned_required = removeElement(raw_schema.required, ignore_param) + raw_schema.put("required", cleaned_required) + } + } + return raw_schema + } + + // + // Clean and check parameters relative to Nextflow native classes + // + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + // + // This function tries to read a JSON params file + // + private static LinkedHashMap paramsLoad(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = paramsRead(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + // + // Method to actually read in JSON file using Groovy. + // Group (as Key), values are all parameters + // - Parameter1 as Key, Description as Value + // - Parameter2 as Key, Description as Value + // .... + // Group + // - + private static LinkedHashMap paramsRead(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + // + // Get maximum number of characters across all parameter names + // + private static Integer paramsMaxChars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } +} diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy new file mode 100755 index 00000000..06499409 --- /dev/null +++ b/lib/NfcoreTemplate.groovy @@ -0,0 +1,258 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute enviroment but can be acheived via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/lib/Utils.groovy b/lib/Utils.groovy new file mode 100755 index 00000000..18173e98 --- /dev/null +++ b/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + + // + // Join module args with appropriate spacing + // + public static String joinModuleArgs(args_list) { + return ' ' + args_list.join(' ') + } +} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy new file mode 100755 index 00000000..43e5694a --- /dev/null +++ b/lib/WorkflowMain.groovy @@ -0,0 +1,94 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/rnavar pipeline +// + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + // + // Print help to screen if required + // + public static String help(workflow, params, log) { + def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + def help_string = '' + help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) + help_string += NfcoreSchema.paramsHelp(workflow, params, command) + help_string += '\n' + citation(workflow) + '\n' + help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) + return help_string + } + + // + // Print parameter summary log to screen + // + public static String paramsSummaryLog(workflow, params, log) { + def summary_log = '' + summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) + summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) + summary_log += '\n' + citation(workflow) + '\n' + summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) + return summary_log + } + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log) { + // Print help to screen if required + if (params.help) { + log.info help(workflow, params, log) + System.exit(0) + } + + // Validate workflow parameters via the JSON schema + if (params.validate_params) { + NfcoreSchema.validateParameters(workflow, params, log) + } + + // Print parameter summary log to screen + log.info paramsSummaryLog(workflow, params, log) + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + + // Check that conda channels are set-up correctly + if (params.enable_conda) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" + System.exit(1) + } + } + + // + // Get attribute from genome config file e.g. fasta + // + public static String getGenomeAttribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy new file mode 100755 index 00000000..15dd1328 --- /dev/null +++ b/lib/WorkflowRnavar.groovy @@ -0,0 +1,59 @@ +// +// This file holds several functions specific to the workflow/rnavar.nf in the nf-core/rnavar pipeline +// + +class WorkflowRnavar { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + genomeExistsError(params, log) + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += " \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "===================================================================================" + System.exit(1) + } + } +} diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar new file mode 100644 index 0000000000000000000000000000000000000000..805c8bb5e4fd43a12a5891eea5a68788309629b0 GIT binary patch literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh 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https://github.com/nf-core/rnavar + Website: https://nf-co.re/rnavar + Slack : https://nfcore.slack.com/channels/rnavar +---------------------------------------------------------------------------------------- +*/ + +nextflow.enable.dsl = 2 + +/* +======================================================================================== + GENOME PARAMETER VALUES +======================================================================================== +*/ + +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') + +/* +======================================================================================== + VALIDATE & PRINT PARAMETER SUMMARY +======================================================================================== +*/ + +WorkflowMain.initialise(workflow, params, log) + +/* +======================================================================================== + NAMED WORKFLOW FOR PIPELINE +======================================================================================== +*/ + +include { RNAVAR } from './workflows/rnavar' + +// +// WORKFLOW: Run main nf-core/rnavar analysis pipeline +// +workflow NFCORE_RNAVAR { + RNAVAR () +} + +/* +======================================================================================== + RUN ALL WORKFLOWS +======================================================================================== +*/ + +// +// WORKFLOW: Execute a single named workflow for the pipeline +// See: https://github.com/nf-core/rnaseq/issues/619 +// +workflow { + NFCORE_RNAVAR () +} + +/* +======================================================================================== + THE END +======================================================================================== +*/ diff --git a/modules.json b/modules.json new file mode 100644 index 00000000..5dce8a34 --- /dev/null +++ b/modules.json @@ -0,0 +1,17 @@ +{ + "name": "nf-core/rnavar", + "homePage": "https://github.com/nf-core/rnavar", + "repos": { + "nf-core/modules": { + "custom/dumpsoftwareversions": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "fastqc": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "multiqc": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + } + } + } +} \ No newline at end of file diff --git a/modules/local/functions.nf b/modules/local/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/local/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf new file mode 100644 index 00000000..b9fa2ac9 --- /dev/null +++ b/modules/local/get_chrom_sizes.nf @@ -0,0 +1,40 @@ +// Import generic module functions +include { saveFiles; getProcessName } from './functions' + +params.options = [:] + +process GET_CHROM_SIZES { + tag "$fasta" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + } else { + container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + } + + input: + path fasta + + output: + path '*.sizes' , emit: sizes + path '*.fai' , emit: fai + path "versions.yml", emit: versions + + script: + """ + samtools \\ + faidx \\ + $fasta + + cut -f 1,2 ${fasta}.fai > ${fasta}.sizes + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf new file mode 100644 index 00000000..ea2519c8 --- /dev/null +++ b/modules/local/gtf2bed.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { saveFiles; getProcessName } from './functions' + +params.options = [:] + +process GTF2BED { + tag "$gtf" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/perl:5.26.2" + } else { + container "quay.io/biocontainers/perl:5.26.2" + } + + input: + path gtf + + output: + path '*.bed' , emit: bed + path "versions.yml", emit: versions + + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ + """ + gtf2bed \\ + $gtf \\ + > ${gtf.baseName}.bed + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + perl: \$(echo \$(perl --version 2>&1) | sed 's/.*v\\(.*\\)) built.*/\\1/') + END_VERSIONS + """ +} diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf new file mode 100644 index 00000000..79088854 --- /dev/null +++ b/modules/local/gtf_gene_filter.nf @@ -0,0 +1,39 @@ +// Import generic module functions +include { saveFiles; getProcessName } from './functions' + +params.options = [:] + +process GTF_GENE_FILTER { + tag "$fasta" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path fasta + path gtf + + output: + path "*.gtf" , emit: gtf + path "versions.yml", emit: versions + + script: // filter_gtf_for_genes_in_genome.py is bundled with the pipeline, in nf-core/rnaseq/bin/ + """ + filter_gtf_for_genes_in_genome.py \\ + --gtf $gtf \\ + --fasta $fasta \\ + -o ${fasta.baseName}_genes.gtf + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf new file mode 100644 index 00000000..dc0dfc7b --- /dev/null +++ b/modules/local/samplesheet_check.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { saveFiles; getProcessName } from './functions' + +params.options = [:] + +process SAMPLESHEET_CHECK { + tag "$samplesheet" + label "process_medium" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + input: + path samplesheet + + output: + path '*.csv' , emit: csv + path "versions.yml", emit: versions + + script: // This script is bundled with the pipeline, in nf-core/rnavar/bin/ + """ + check_samplesheet.py \\ + $samplesheet \\ + samplesheet.valid.csv + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + python: \$(python --version | sed 's/Python //g') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf new file mode 100644 index 00000000..faf2073f --- /dev/null +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf @@ -0,0 +1,106 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process CUSTOM_DUMPSOFTWAREVERSIONS { + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } + + // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container + conda (params.enable_conda ? "bioconda::multiqc=1.11" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" + } else { + container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" + } + + input: + path versions + + output: + path "software_versions.yml" , emit: yml + path "software_versions_mqc.yml", emit: mqc_yml + path "versions.yml" , emit: versions + + script: + """ + #!/usr/bin/env python + + import yaml + import platform + from textwrap import dedent + + def _make_versions_html(versions): + html = [ + dedent( + '''\\ + + + + + + + + + + ''' + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f'''\\ + + + + + + ''' + ) + ) + html.append("") + html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") + return "\\n".join(html) + + module_versions = {} + module_versions["${getProcessName(task.process)}"] = { + 'python': platform.python_version(), + 'yaml': yaml.__version__ + } + + with open("$versions") as f: + workflow_versions = yaml.load(f, Loader=yaml.BaseLoader) | module_versions + + workflow_versions["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version" + } + + versions_mqc = { + 'id': 'software_versions', + 'section_name': '${workflow.manifest.name} Software Versions', + 'section_href': 'https://github.com/${workflow.manifest.name}', + 'plot_type': 'html', + 'description': 'are collected at run time from the software output.', + 'data': _make_versions_html(workflow_versions) + } + + with open("software_versions.yml", 'w') as f: + yaml.dump(workflow_versions, f, default_flow_style=False) + with open("software_versions_mqc.yml", 'w') as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open('versions.yml', 'w') as f: + yaml.dump(module_versions, f, default_flow_style=False) + """ +} diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml new file mode 100644 index 00000000..c8310e35 --- /dev/null +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml @@ -0,0 +1,33 @@ +name: custom_dumpsoftwareversions +description: Custom module used to dump software versions within the nf-core pipeline template +keywords: + - custom + - version +tools: + - custom: + description: Custom module used to dump software versions within the nf-core pipeline template + homepage: https://github.com/nf-core/tools + documentation: https://github.com/nf-core/tools + licence: ['MIT'] +input: + - versions: + type: file + description: YML file containing software versions + pattern: "*.yml" + +output: + - yml: + type: file + description: Standard YML file containing software versions + pattern: "software_versions.yml" + - mqc_yml: + type: file + description: MultiQC custom content YML file containing software versions + pattern: "software_versions_mqc.yml" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@drpatelh" diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/fastqc/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf new file mode 100644 index 00000000..9f6cfc55 --- /dev/null +++ b/modules/nf-core/modules/fastqc/main.nf @@ -0,0 +1,54 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process FASTQC { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" + } else { + container "quay.io/biocontainers/fastqc:0.11.9--0" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "versions.yml" , emit: versions + + script: + // Add soft-links to original FastQs for consistent naming in pipeline + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$( fastqc --version | sed -e "s/FastQC v//g" ) + END_VERSIONS + """ + } else { + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$( fastqc --version | sed -e "s/FastQC v//g" ) + END_VERSIONS + """ + } +} diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml new file mode 100644 index 00000000..b09553a3 --- /dev/null +++ b/modules/nf-core/modules/fastqc/meta.yml @@ -0,0 +1,52 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ + licence: ['GPL-2.0-only'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/multiqc/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf new file mode 100644 index 00000000..0861aa59 --- /dev/null +++ b/modules/nf-core/modules/multiqc/main.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process MULTIQC { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" + } else { + container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" + } + + input: + path multiqc_files + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "versions.yml" , emit: versions + + script: + """ + multiqc -f $options.args . + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml new file mode 100644 index 00000000..63c75a45 --- /dev/null +++ b/modules/nf-core/modules/multiqc/meta.yml @@ -0,0 +1,40 @@ +name: MultiQC +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ + licence: ['GPL-3.0-or-later'] +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: dir + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" diff --git a/nextflow.config b/nextflow.config new file mode 100644 index 00000000..439746a0 --- /dev/null +++ b/nextflow.config @@ -0,0 +1,197 @@ +/* +======================================================================================== + nf-core/rnavar Nextflow config file +======================================================================================== + Default config options for all compute environments +---------------------------------------------------------------------------------------- +*/ + +// Global default params, used in configs +params { + + // TODO nf-core: Specify your pipeline's command line flags + // Input options + input = null + + // References + genome = null + igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_ignore = false + + // MultiQC options + multiqc_config = null + multiqc_title = null + max_multiqc_email_size = '25.MB' + + // Boilerplate options + outdir = './results' + tracedir = "${params.outdir}/pipeline_info" + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + help = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,modules' + enable_conda = false + singularity_pull_docker_container = false + + // Config options + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + config_profile_description = null + config_profile_contact = null + config_profile_url = null + config_profile_name = null + + // Max resource options + // Defaults only, expecting to be overwritten + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' + +} + +// Load base.config by default for all pipelines +includeConfig 'conf/base.config' + +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + +// Load nf-core custom profiles from different Institutions +try { + includeConfig "${params.custom_config_base}/nfcore_custom.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") +} + +// Load igenomes.config if required +if (!params.igenomes_ignore) { + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} + +profiles { + debug { process.beforeScript = 'echo $HOSTNAME' } + conda { + params.enable_conda = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + docker { + docker.enabled = true + docker.userEmulation = true + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + singularity { + singularity.enabled = true + singularity.autoMounts = true + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + podman { + podman.enabled = true + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + shifter { + shifter.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + } + charliecloud { + charliecloud.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } +} + +// Export these variables to prevent local Python/R libraries from conflicting with those in the container +env { + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" +} + +// Capture exit codes from upstream processes when piping +process.shell = ['/bin/bash', '-euo', 'pipefail'] + +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') +timeline { + enabled = true + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" +} +report { + enabled = true + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" +} +trace { + enabled = true + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" +} +dag { + enabled = true + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" +} + +manifest { + name = 'nf-core/rnavar' + author = '@praveenraj2018' + homePage = 'https://github.com/nf-core/rnavar' + description = 'GATK4 RNA variant calling pipeline' + mainScript = 'main.nf' + nextflowVersion = '!>=21.04.0' + version = '1.0dev' +} + +// Function to ensure that resource requirements don't go beyond +// a maximum limit +def check_max(obj, type) { + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } + } +} diff --git a/nextflow_schema.json b/nextflow_schema.json new file mode 100644 index 00000000..d3eb24a9 --- /dev/null +++ b/nextflow_schema.json @@ -0,0 +1,285 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/nextflow_schema.json", + "title": "nf-core/rnavar pipeline parameters", + "description": "GATK4 RNA variant calling pipeline", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": [ + "input" + ], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "^\\S+\\.csv$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/rnavar/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" + }, + "outdir": { + "type": "string", + "description": "Path to the output directory where the results will be saved.", + "default": "./results", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", + "hidden": true, + "fa_icon": "fas fa-toolbox", + "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf new file mode 100644 index 00000000..c1b07196 --- /dev/null +++ b/subworkflows/local/input_check.nf @@ -0,0 +1,44 @@ +// +// Check input samplesheet and get read channels +// + +params.options = [:] + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .csv + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channels(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] + versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def create_fastq_channels(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf new file mode 100644 index 00000000..a4e52e39 --- /dev/null +++ b/workflows/rnavar.nf @@ -0,0 +1,129 @@ +/* +======================================================================================== + VALIDATE INPUTS +======================================================================================== +*/ + +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) + +// Validate input parameters +WorkflowRnavar.initialise(params, log) + +// TODO nf-core: Add all file path parameters for the pipeline to the list below +// Check input path parameters to see if they exist +def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +/* +======================================================================================== + CONFIG FILES +======================================================================================== +*/ + +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. +def modules = params.modules.clone() + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) + +/* +======================================================================================== + IMPORT NF-CORE MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' + +// +// MODULE: Installed directly from nf-core/modules +// +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' addParams( options: [publish_files : ['_versions.yml':'']] ) + +/* +======================================================================================== + RUN MAIN WORKFLOW +======================================================================================== +*/ + +// Info required for completion email and summary +def multiqc_report = [] + +workflow RNAVAR { + + ch_versions = Channel.empty() + + // + // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // + INPUT_CHECK ( + ch_input + ) + ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + + // + // MODULE: Run FastQC + // + FASTQC ( + INPUT_CHECK.out.reads + ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + + CUSTOM_DUMPSOFTWAREVERSIONS ( + ch_versions.unique().collectFile(name: 'collated_versions.yml') + ) + + // + // MODULE: MultiQC + // + workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect() + ) + multiqc_report = MULTIQC.out.report.toList() + ch_versions = ch_versions.mix(MULTIQC.out.versions) +} + +/* +======================================================================================== + COMPLETION EMAIL AND SUMMARY +======================================================================================== +*/ + +workflow.onComplete { + if (params.email || params.email_on_fail) { + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + } + NfcoreTemplate.summary(workflow, params, log) +} + +/* +======================================================================================== + THE END +======================================================================================== +*/ From e20873f69a35c84e125e1a2b531f82216c3fb2e7 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 11 Nov 2021 18:01:12 +0100 Subject: [PATCH 043/235] cleanup --- modules.json | 51 ++++++++ modules/local/gatk4/applybqsr/functions.nf | 68 +++++++++++ modules/local/gatk4/applybqsr/main.nf | 46 +++++++ modules/local/gatk4/applybqsr/meta.yml | 58 +++++++++ .../local/gatk4/haplotypecaller/functions.nf | 68 +++++++++++ modules/local/gatk4/haplotypecaller/main.nf | 60 +++++++++ modules/local/gatk4/haplotypecaller/meta.yml | 63 ++++++++++ modules/local/gatk4/mergevcfs/functions.nf | 68 +++++++++++ modules/local/gatk4/mergevcfs/main.nf | 49 ++++++++ modules/local/gatk4/mergevcfs/meta.yml | 42 +++++++ .../local/gatk4/splitncigarreads/functions.nf | 68 +++++++++++ modules/local/gatk4/splitncigarreads/main.nf | 43 +++++++ modules/local/gatk4/splitncigarreads/meta.yml | 40 ++++++ .../gatk4/variantfiltration/functions.nf | 68 +++++++++++ modules/local/gatk4/variantfiltration/main.nf | 44 +++++++ .../local/gatk4/variantfiltration/meta.yml | 47 ++++++++ .../nf-core/modules/cat/fastq/functions.nf | 78 ++++++++++++ modules/nf-core/modules/cat/fastq/main.nf | 58 +++++++++ modules/nf-core/modules/cat/fastq/meta.yml | 39 ++++++ .../gatk4/baserecalibrator/functions.nf | 78 ++++++++++++ .../modules/gatk4/baserecalibrator/main.nf | 59 +++++++++ .../modules/gatk4/baserecalibrator/meta.yml | 64 ++++++++++ .../gatk4/bedtointervallist/functions.nf | 78 ++++++++++++ .../modules/gatk4/bedtointervallist/main.nf | 43 +++++++ .../modules/gatk4/bedtointervallist/meta.yml | 40 ++++++ .../createsequencedictionary/functions.nf | 78 ++++++++++++ .../gatk4/createsequencedictionary/main.nf | 47 ++++++++ .../gatk4/createsequencedictionary/meta.yml | 32 +++++ .../gatk4/intervallisttools/functions.nf | 78 ++++++++++++ .../modules/gatk4/intervallisttools/main.nf | 55 +++++++++ .../modules/gatk4/intervallisttools/meta.yml | 47 ++++++++ modules/nf-core/modules/gffread/functions.nf | 78 ++++++++++++ modules/nf-core/modules/gffread/main.nf | 40 ++++++ modules/nf-core/modules/gffread/meta.yml | 33 +++++ modules/nf-core/modules/gunzip/functions.nf | 78 ++++++++++++ modules/nf-core/modules/gunzip/main.nf | 41 +++++++ modules/nf-core/modules/gunzip/meta.yml | 28 +++++ modules/nf-core/modules/gunzip/test.txt.gz | Bin 0 -> 47 bytes .../modules/samtools/faidx/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/faidx/main.nf | 36 ++++++ .../nf-core/modules/samtools/faidx/meta.yml | 33 +++++ .../modules/samtools/flagstat/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/flagstat/main.nf | 36 ++++++ .../modules/samtools/flagstat/meta.yml | 49 ++++++++ .../modules/samtools/idxstats/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/idxstats/main.nf | 36 ++++++ .../modules/samtools/idxstats/meta.yml | 50 ++++++++ .../modules/samtools/index/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/index/main.nf | 38 ++++++ .../nf-core/modules/samtools/index/meta.yml | 53 ++++++++ .../modules/samtools/merge/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/merge/main.nf | 41 +++++++ .../nf-core/modules/samtools/merge/meta.yml | 54 +++++++++ .../modules/samtools/sort/functions.nf | 78 ++++++++++++ modules/nf-core/modules/samtools/sort/main.nf | 37 ++++++ .../nf-core/modules/samtools/sort/meta.yml | 44 +++++++ .../modules/samtools/stats/functions.nf | 78 ++++++++++++ .../nf-core/modules/samtools/stats/main.nf | 38 ++++++ .../nf-core/modules/samtools/stats/meta.yml | 53 ++++++++ .../nf-core/modules/star/align/functions.nf | 78 ++++++++++++ modules/nf-core/modules/star/align/main.nf | 75 ++++++++++++ modules/nf-core/modules/star/align/meta.yml | 81 +++++++++++++ .../modules/star/genomegenerate/functions.nf | 78 ++++++++++++ .../modules/star/genomegenerate/main.nf | 76 ++++++++++++ .../modules/star/genomegenerate/meta.yml | 37 ++++++ modules/nf-core/modules/untar/functions.nf | 78 ++++++++++++ modules/nf-core/modules/untar/main.nf | 41 +++++++ modules/nf-core/modules/untar/meta.yml | 28 +++++ subworkflows/local/prepare_genome.nf | 114 ++++++++++++++++++ subworkflows/nf-core/align_star.nf | 50 ++++++++ subworkflows/nf-core/bam_sort_samtools.nf | 45 +++++++ subworkflows/nf-core/bam_stats_samtools.nf | 25 ++++ subworkflows/nf-core/markduplicates.nf | 47 ++++++++ subworkflows/nf-core/recalibrate.nf | 69 +++++++++++ subworkflows/nf-core/splitn_cigar_reads.nf | 55 +++++++++ workflows/rnavar.nf | 67 +++++++++- 76 files changed, 4242 insertions(+), 1 deletion(-) create mode 100644 modules/local/gatk4/applybqsr/functions.nf create mode 100644 modules/local/gatk4/applybqsr/main.nf create mode 100644 modules/local/gatk4/applybqsr/meta.yml create mode 100644 modules/local/gatk4/haplotypecaller/functions.nf create mode 100644 modules/local/gatk4/haplotypecaller/main.nf create mode 100644 modules/local/gatk4/haplotypecaller/meta.yml create mode 100644 modules/local/gatk4/mergevcfs/functions.nf create mode 100644 modules/local/gatk4/mergevcfs/main.nf create mode 100644 modules/local/gatk4/mergevcfs/meta.yml create mode 100644 modules/local/gatk4/splitncigarreads/functions.nf create mode 100644 modules/local/gatk4/splitncigarreads/main.nf create mode 100644 modules/local/gatk4/splitncigarreads/meta.yml create mode 100644 modules/local/gatk4/variantfiltration/functions.nf create mode 100644 modules/local/gatk4/variantfiltration/main.nf create mode 100644 modules/local/gatk4/variantfiltration/meta.yml create mode 100644 modules/nf-core/modules/cat/fastq/functions.nf create mode 100644 modules/nf-core/modules/cat/fastq/main.nf create mode 100644 modules/nf-core/modules/cat/fastq/meta.yml create mode 100644 modules/nf-core/modules/gatk4/baserecalibrator/functions.nf create mode 100644 modules/nf-core/modules/gatk4/baserecalibrator/main.nf create mode 100644 modules/nf-core/modules/gatk4/baserecalibrator/meta.yml create mode 100644 modules/nf-core/modules/gatk4/bedtointervallist/functions.nf create mode 100644 modules/nf-core/modules/gatk4/bedtointervallist/main.nf create mode 100644 modules/nf-core/modules/gatk4/bedtointervallist/meta.yml create mode 100644 modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf create mode 100644 modules/nf-core/modules/gatk4/createsequencedictionary/main.nf create mode 100644 modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml create mode 100644 modules/nf-core/modules/gatk4/intervallisttools/functions.nf create mode 100644 modules/nf-core/modules/gatk4/intervallisttools/main.nf create mode 100644 modules/nf-core/modules/gatk4/intervallisttools/meta.yml create mode 100644 modules/nf-core/modules/gffread/functions.nf create mode 100644 modules/nf-core/modules/gffread/main.nf create mode 100644 modules/nf-core/modules/gffread/meta.yml create mode 100644 modules/nf-core/modules/gunzip/functions.nf create mode 100644 modules/nf-core/modules/gunzip/main.nf create mode 100644 modules/nf-core/modules/gunzip/meta.yml create mode 100644 modules/nf-core/modules/gunzip/test.txt.gz create mode 100644 modules/nf-core/modules/samtools/faidx/functions.nf create mode 100644 modules/nf-core/modules/samtools/faidx/main.nf create mode 100644 modules/nf-core/modules/samtools/faidx/meta.yml create mode 100644 modules/nf-core/modules/samtools/flagstat/functions.nf create mode 100644 modules/nf-core/modules/samtools/flagstat/main.nf create mode 100644 modules/nf-core/modules/samtools/flagstat/meta.yml create mode 100644 modules/nf-core/modules/samtools/idxstats/functions.nf create mode 100644 modules/nf-core/modules/samtools/idxstats/main.nf create mode 100644 modules/nf-core/modules/samtools/idxstats/meta.yml create mode 100644 modules/nf-core/modules/samtools/index/functions.nf create mode 100644 modules/nf-core/modules/samtools/index/main.nf create mode 100644 modules/nf-core/modules/samtools/index/meta.yml create mode 100644 modules/nf-core/modules/samtools/merge/functions.nf create mode 100644 modules/nf-core/modules/samtools/merge/main.nf create mode 100644 modules/nf-core/modules/samtools/merge/meta.yml create mode 100644 modules/nf-core/modules/samtools/sort/functions.nf create mode 100644 modules/nf-core/modules/samtools/sort/main.nf create mode 100644 modules/nf-core/modules/samtools/sort/meta.yml create mode 100644 modules/nf-core/modules/samtools/stats/functions.nf create mode 100644 modules/nf-core/modules/samtools/stats/main.nf create mode 100644 modules/nf-core/modules/samtools/stats/meta.yml create mode 100644 modules/nf-core/modules/star/align/functions.nf create mode 100644 modules/nf-core/modules/star/align/main.nf create mode 100644 modules/nf-core/modules/star/align/meta.yml create mode 100644 modules/nf-core/modules/star/genomegenerate/functions.nf create mode 100644 modules/nf-core/modules/star/genomegenerate/main.nf create mode 100644 modules/nf-core/modules/star/genomegenerate/meta.yml create mode 100644 modules/nf-core/modules/untar/functions.nf create mode 100644 modules/nf-core/modules/untar/main.nf create mode 100644 modules/nf-core/modules/untar/meta.yml create mode 100644 subworkflows/local/prepare_genome.nf create mode 100644 subworkflows/nf-core/align_star.nf create mode 100644 subworkflows/nf-core/bam_sort_samtools.nf create mode 100644 subworkflows/nf-core/bam_stats_samtools.nf create mode 100644 subworkflows/nf-core/markduplicates.nf create mode 100644 subworkflows/nf-core/recalibrate.nf create mode 100644 subworkflows/nf-core/splitn_cigar_reads.nf diff --git a/modules.json b/modules.json index 5dce8a34..d91398e0 100644 --- a/modules.json +++ b/modules.json @@ -3,14 +3,65 @@ "homePage": "https://github.com/nf-core/rnavar", "repos": { "nf-core/modules": { + "cat/fastq": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, "custom/dumpsoftwareversions": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, "fastqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, + "gatk4/baserecalibrator": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, + "gatk4/bedtointervallist": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, + "gatk4/createsequencedictionary": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, + "gatk4/intervallisttools": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, + "gffread": { + "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" + }, + "gunzip": { + "git_sha": "e560fbbc3ed68e62bc810a50f552394056f81762" + }, "multiqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "samtools/faidx": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "samtools/flagstat": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "samtools/idxstats": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "samtools/index": { + "git_sha": "1abe23e1592f7cf6a3724589061827df0d8a0252" + }, + "samtools/merge": { + "git_sha": "ac1e6df076195cec553a2079c9cebd94026a0d47" + }, + "samtools/sort": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "samtools/stats": { + "git_sha": "ac1e6df076195cec553a2079c9cebd94026a0d47" + }, + "star/align": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "star/genomegenerate": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, + "untar": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" } } } diff --git a/modules/local/gatk4/applybqsr/functions.nf b/modules/local/gatk4/applybqsr/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/gatk4/applybqsr/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/gatk4/applybqsr/main.nf b/modules/local/gatk4/applybqsr/main.nf new file mode 100644 index 00000000..8e10a5f2 --- /dev/null +++ b/modules/local/gatk4/applybqsr/main.nf @@ -0,0 +1,46 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_APPLYBQSR { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervals) + path fasta + path fastaidx + path dict + + output: + tuple val(meta), path("*.bam"), emit: bam + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def interval = intervals ? "-L ${intervals}" : "" + """ + gatk ApplyBQSR \\ + -R $fasta \\ + -I $bam \\ + --bqsr-recal-file $bqsr_table \\ + $interval \\ + -O ${prefix}.bam \\ + $options.args + + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/local/gatk4/applybqsr/meta.yml b/modules/local/gatk4/applybqsr/meta.yml new file mode 100644 index 00000000..9bf12f09 --- /dev/null +++ b/modules/local/gatk4/applybqsr/meta.yml @@ -0,0 +1,58 @@ +name: gatk4_applybqsr +description: Apply base quality score recalibration (BQSR) to a bam file +keywords: + - bqsr + - bam +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file from alignment + pattern: "*.{bam}" + - bqsr_table: + type: file + description: Recalibration table from gatk4_baserecalibrator + - fasta: + type: file + description: The reference fasta file + - fastaidx: + type: file + description: Index of reference fasta file + - dict: + type: file + description: GATK sequence dictionary + - intervalsBed: + type: file + description: Bed file with the genomic regions included in the library (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - bam: + type: file + description: Recalibrated BAM file + pattern: "*.{bam}" + +authors: + - "@yocra3" diff --git a/modules/local/gatk4/haplotypecaller/functions.nf b/modules/local/gatk4/haplotypecaller/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/gatk4/haplotypecaller/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf new file mode 100644 index 00000000..da90ba4b --- /dev/null +++ b/modules/local/gatk4/haplotypecaller/main.nf @@ -0,0 +1,60 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_HAPLOTYPECALLER { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam), path(bai), path(interval) + path dbsnp + path dbsnp_tbi + path dict + path fasta + path fai + val no_intervals + + output: + tuple val(meta), path("*.vcf") , emit: vcf + tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + def intervalsOptions = no_intervals ? "" : "-L ${interval}" + def dbsnpOptions = dbsnp ? "-D ${dbsnp}" : "" + //TODO allow ploidy argument here since we allow it for the cnv callers? or is this covered with options? Might unintuitive to use + """ + gatk \\ + --java-options "-Xmx${avail_mem}g" \\ + HaplotypeCaller \\ + -R $fasta \\ + -I $bam \\ + ${dbsnpOptions} \\ + ${intervalsOptions} \\ + -O ${prefix}.vcf \\ + $options.args + + gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + """ +} diff --git a/modules/local/gatk4/haplotypecaller/meta.yml b/modules/local/gatk4/haplotypecaller/meta.yml new file mode 100644 index 00000000..f0fc3910 --- /dev/null +++ b/modules/local/gatk4/haplotypecaller/meta.yml @@ -0,0 +1,63 @@ +name: gatk4_haplotypecaller +description: Call germline SNPs and indels via local re-assembly of haplotypes +keywords: + - gatk4 + - haplotypecaller + - haplotype +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.bam" + - bai: + type: file + description: Index of BAM file + pattern: "*.bam.bai" + - fasta: + type: file + description: The reference fasta file + pattern: "*.fasta" + - fai: + type: file + description: Index of reference fasta file + pattern: "fasta.fai" + - dict: + type: file + description: GATK sequence dictionary + pattern: "*.dict" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" + - vcf: + type: file + description: Compressed VCF file + pattern: "*.vcf.gz" + - tbi: + type: file + description: Index of VCF file + pattern: "*.vcf.gz.tbi" + +authors: + - "@suzannejin" diff --git a/modules/local/gatk4/mergevcfs/functions.nf b/modules/local/gatk4/mergevcfs/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/gatk4/mergevcfs/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/gatk4/mergevcfs/main.nf b/modules/local/gatk4/mergevcfs/main.nf new file mode 100644 index 00000000..8b9747bc --- /dev/null +++ b/modules/local/gatk4/mergevcfs/main.nf @@ -0,0 +1,49 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_MERGEVCFS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(vcfs) + path ref_dict + val use_ref_dict + + output: + tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi'), emit: vcf + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + + // Make list of VCFs to merge + def input = "" + for (vcf in vcfs) { + input += " I=${vcf}" + } + def ref = use_ref_dict ? "D=${ref_dict}" : "" + """ + gatk MergeVcfs \\ + $input \\ + O=${prefix}.vcf.gz \\ + $ref \\ + $options.args + + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/local/gatk4/mergevcfs/meta.yml b/modules/local/gatk4/mergevcfs/meta.yml new file mode 100644 index 00000000..14b28fa0 --- /dev/null +++ b/modules/local/gatk4/mergevcfs/meta.yml @@ -0,0 +1,42 @@ +name: gatk4_mergevcfs +description: Merges several vcf files +keywords: + - vcf + - merge +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - vcf: + type: list + description: Two or more VCF files + pattern: "*.{vcf,vcf.gz}" + - ref_dict: + type: file + description: Optional Sequence Dictionary as input + pattern: "*.dict" + - use_ref_dict: + type: boolean + description: Specify whether or not to use a given reference dictionary +output: + - vcf: + type: file + description: merged vcf file + pattern: "*.vcf.gz" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/modules/local/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/gatk4/splitncigarreads/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/gatk4/splitncigarreads/main.nf b/modules/local/gatk4/splitncigarreads/main.nf new file mode 100644 index 00000000..d1c328ea --- /dev/null +++ b/modules/local/gatk4/splitncigarreads/main.nf @@ -0,0 +1,43 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_SPLITNCIGARREADS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(bam) + path(fasta) + path(fai) + path(dict) + + output: + tuple val(meta), path('*.bam'), path('*.bai'), emit: bam + path '*.version.txt' , emit: version + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + gatk SplitNCigarReads \\ + -R $fasta \\ + -I $bam \\ + -O ${prefix}.bam \\ + $options.args + + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/local/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml new file mode 100644 index 00000000..e433cbf6 --- /dev/null +++ b/modules/local/gatk4/splitncigarreads/meta.yml @@ -0,0 +1,40 @@ +name: gatk4_splitncigarreads +description: Splits reads that contain Ns in their cigar string +keywords: + - vcf + - merge +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - bam: + type: list + description: BAM/SAM/CRAM file containing reads + pattern: "*.{bam,sam,cram}" + - fasta: + type: tuple of files + description: | + Tuple of fasta file (first), sequence dict (second) and fasta index (third) + pattern: ["*.fasta", "*.dict", "*.fai"] +output: + - bam: + type: file + description: Output file with split reads (BAM/SAM/CRAM) + pattern: "*.{bam,sam,cram}" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/modules/local/gatk4/variantfiltration/functions.nf b/modules/local/gatk4/variantfiltration/functions.nf new file mode 100644 index 00000000..da9da093 --- /dev/null +++ b/modules/local/gatk4/variantfiltration/functions.nf @@ -0,0 +1,68 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/modules/local/gatk4/variantfiltration/main.nf b/modules/local/gatk4/variantfiltration/main.nf new file mode 100644 index 00000000..d442aba8 --- /dev/null +++ b/modules/local/gatk4/variantfiltration/main.nf @@ -0,0 +1,44 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_VARIANTFILTRATION { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + } else { + container "quay.io/biocontainers/gatk4:4.2.0.0--0" + } + + input: + tuple val(meta), path(vcf), path (tbi) + path fasta + path fai + path dict + + output: + tuple val(meta), path("*.vcf"), emit: vcf + path "*.version.txt" , emit: version + + + script: + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + gatk VariantFiltration \\ + -R $fasta \\ + -V $vcf \\ + -O ${prefix}.vcf \\ + $options.args + + echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + """ +} diff --git a/modules/local/gatk4/variantfiltration/meta.yml b/modules/local/gatk4/variantfiltration/meta.yml new file mode 100644 index 00000000..d7f72582 --- /dev/null +++ b/modules/local/gatk4/variantfiltration/meta.yml @@ -0,0 +1,47 @@ +name: gatk4_variantfiltration +description: Filter variants +keywords: + - vcf + - filter +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - vcf: + type: list + description: Input VCF file + pattern: "*.{vcf}" + - fasta: + type: file + description: Fasta file of reference genome + pattern: "*.fasta" + - fai: + type: file + description: Index of fasta file + pattern: "*.fasta.fai" + - dict: + type: file + description: Sequence dictionary of fastea file + pattern: "*.dict" +output: + - vcf: + type: file + description: filtered VCF file + pattern: "*.filtered.{vcf}" + - version: + type: file + description: File containing software version + pattern: "*.version.txt" +authors: + - "@kevinmenden" diff --git a/modules/nf-core/modules/cat/fastq/functions.nf b/modules/nf-core/modules/cat/fastq/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf new file mode 100644 index 00000000..538915a7 --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/main.nf @@ -0,0 +1,58 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process CAT_FASTQ { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.merged.fastq.gz"), emit: reads + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def readList = reads.collect{ it.toString() } + if (meta.single_end) { + if (readList.size > 1) { + """ + cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') + END_VERSIONS + """ + } + } else { + if (readList.size > 2) { + def read1 = [] + def read2 = [] + readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } + """ + cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') + END_VERSIONS + """ + } + } +} diff --git a/modules/nf-core/modules/cat/fastq/meta.yml b/modules/nf-core/modules/cat/fastq/meta.yml new file mode 100644 index 00000000..1992fa34 --- /dev/null +++ b/modules/nf-core/modules/cat/fastq/meta.yml @@ -0,0 +1,39 @@ +name: cat_fastq +description: Concatenates fastq files +keywords: + - fastq + - concatenate +tools: + - cat: + description: | + The cat utility reads files sequentially, writing them to the standard output. + documentation: https://www.gnu.org/software/coreutils/manual/html_node/cat-invocation.html + licence: ['GPL-3.0-or-later'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FastQ files to be concatenated. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: Merged fastq file + pattern: "*.{merged.fastq.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf b/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf new file mode 100644 index 00000000..ff9eb1f9 --- /dev/null +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -0,0 +1,59 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_BASERECALIBRATOR { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + tuple val(meta), path(input), path(input_index) + path fasta + path fastaidx + path dict + path intervalsBed + path knownSites + path knownSites_tbi + + output: + tuple val(meta), path("*.table"), emit: table + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') + + if (!task.memory) { + log.info '[GATK BaseRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk BaseRecalibrator \ + -R $fasta \ + -I $input \ + $sitesCommand \ + $intervalsCommand \ + --tmp-dir . \ + $options.args \ + -O ${prefix}.table + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml new file mode 100644 index 00000000..7fd273e1 --- /dev/null +++ b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml @@ -0,0 +1,64 @@ +name: gatk4_baserecalibrator +description: Generate recalibration table for Base Quality Score Recalibration (BQSR) +keywords: + - sort +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] + + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - fasta: + type: file + description: The reference fasta file + - fastaidx: + type: file + description: Index of reference fasta file + - dict: + type: file + description: GATK sequence dictionary + - intervalsBed: + type: file + description: Bed file with the genomic regions included in the library (optional) + - knownSites: + type: file + description: Bed file with the genomic regions included in the library (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - table: + type: file + description: Recalibration table from BaseRecalibrator + pattern: "*.{table}" + +authors: + - "@yocra3" + - "@FriederikeHanssen" diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf new file mode 100644 index 00000000..7c06ccef --- /dev/null +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -0,0 +1,43 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_BEDTOINTERVALLIST { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + tuple val(meta), path(bed) + path sequence_dict + + output: + tuple val(meta), path('*.interval_list'), emit: interval_list + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + gatk BedToIntervalList \\ + -I $bed \\ + -SD $sequence_dict \\ + -O ${prefix}.interval_list \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml b/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml new file mode 100644 index 00000000..910f9552 --- /dev/null +++ b/modules/nf-core/modules/gatk4/bedtointervallist/meta.yml @@ -0,0 +1,40 @@ +name: gatk4_bedtointervallist +description: Creates an interval list from a bed file and a reference dict +keywords: + - bed + - interval list +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - bed: + type: file + description: Input bed file + pattern: "*.bed" + - dict: + type: file + description: Sequence dictionary + pattern: "*.dict" +output: + - interval_list: + type: file + description: gatk interval list file + pattern: "*.interval_list" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@kevinmenden" diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf new file mode 100644 index 00000000..db28e244 --- /dev/null +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_CREATESEQUENCEDICTIONARY { + tag "$fasta" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + path fasta + + output: + path "*.dict" , emit: dict + path "versions.yml" , emit: versions + + script: + def avail_mem = 6 + if (!task.memory) { + log.info '[GATK] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk --java-options "-Xmx${avail_mem}g" \\ + CreateSequenceDictionary \\ + --REFERENCE $fasta \\ + --URI $fasta \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml new file mode 100644 index 00000000..54f479b3 --- /dev/null +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml @@ -0,0 +1,32 @@ +name: gatk4_createsequencedictionary +description: Creates a sequence dictionary for a reference sequence +keywords: + - dictionary + - fasta +tools: + - gatk: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] + +input: + - fasta: + type: file + description: Input fasta file + pattern: "*.{fasta,fa}" +output: + - dict: + type: file + description: gatk dictionary file + pattern: "*.{dict}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" diff --git a/modules/nf-core/modules/gatk4/intervallisttools/functions.nf b/modules/nf-core/modules/gatk4/intervallisttools/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/intervallisttools/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf new file mode 100644 index 00000000..5da651b9 --- /dev/null +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -0,0 +1,55 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_INTERVALLISTTOOLS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + tuple val(meta), path(interval_list) + + output: + tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + + mkdir ${prefix}_split + + gatk \\ + IntervalListTools \\ + -I ${interval_list} \\ + -O ${prefix}_split \\ + $options.args + + python3 < versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/intervallisttools/meta.yml b/modules/nf-core/modules/gatk4/intervallisttools/meta.yml new file mode 100644 index 00000000..9e2d994f --- /dev/null +++ b/modules/nf-core/modules/gatk4/intervallisttools/meta.yml @@ -0,0 +1,47 @@ +name: gatk4_intervallisttools + +description: Splits the interval list file into unique, equally-sized interval files and place it under a directory +keywords: + - sort + - bed + - interval list +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + + - interval_list: + type: file + description: Interval list file + pattern: "*.interval_list" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - interval_list: + type: file + description: Interval list files + pattern: "*.interval_list" + +authors: + - "@praveenraj2018" diff --git a/modules/nf-core/modules/gffread/functions.nf b/modules/nf-core/modules/gffread/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gffread/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gffread/main.nf b/modules/nf-core/modules/gffread/main.nf new file mode 100644 index 00000000..4133ee08 --- /dev/null +++ b/modules/nf-core/modules/gffread/main.nf @@ -0,0 +1,40 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GFFREAD { + tag "$gff" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "bioconda::gffread=0.12.1" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0" + } else { + container "quay.io/biocontainers/gffread:0.12.1--h8b12597_0" + } + + input: + path gff + + output: + path "*.gtf" , emit: gtf + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${gff.baseName}${options.suffix}" : "${gff.baseName}" + """ + gffread \\ + $gff \\ + $options.args \\ + -o ${prefix}.gtf + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(gffread --version 2>&1) + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gffread/meta.yml b/modules/nf-core/modules/gffread/meta.yml new file mode 100644 index 00000000..bf1a15cb --- /dev/null +++ b/modules/nf-core/modules/gffread/meta.yml @@ -0,0 +1,33 @@ +name: gffread +description: Validate, filter, convert and perform various other operations on GFF files +keywords: + - gff + - conversion + - validation +tools: + - gffread: + description: GFF/GTF utility providing format conversions, region filtering, FASTA sequence extraction and more. + homepage: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread + documentation: http://ccb.jhu.edu/software/stringtie/gff.shtml#gffread + tool_dev_url: https://github.com/gpertea/gffread + doi: 10.12688/f1000research.23297.1 + licence: ['MIT'] + +input: + - gff: + type: file + description: A reference file in either the GFF3, GFF2 or GTF format. + pattern: "*.{gff, gtf}" + +output: + - gtf: + type: file + description: GTF file resulting from the conversion of the GFF input file + pattern: "*.{gtf}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@emiller88" diff --git a/modules/nf-core/modules/gunzip/functions.nf b/modules/nf-core/modules/gunzip/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gunzip/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf new file mode 100644 index 00000000..aec4569f --- /dev/null +++ b/modules/nf-core/modules/gunzip/main.nf @@ -0,0 +1,41 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GUNZIP { + tag "$archive" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + path archive + + output: + path "$gunzip", emit: gunzip + path "versions.yml" , emit: versions + + script: + gunzip = archive.toString() - '.gz' + """ + gunzip \\ + -f \\ + $options.args \\ + $archive + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml new file mode 100644 index 00000000..3482f0d2 --- /dev/null +++ b/modules/nf-core/modules/gunzip/meta.yml @@ -0,0 +1,28 @@ +name: gunzip +description: Compresses and decompresses files. +keywords: + - gunzip + - compression +tools: + - gunzip: + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ['GPL-3.0-or-later'] +input: + - archive: + type: file + description: File to be compressed/uncompressed + pattern: "*.*" +output: + - gunzip: + type: file + description: Compressed/uncompressed file + pattern: "*.*" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/modules/gunzip/test.txt.gz b/modules/nf-core/modules/gunzip/test.txt.gz new file mode 100644 index 0000000000000000000000000000000000000000..381417cf643f1b5c547b57b251d71e6d5ce11e16 GIT binary patch literal 47 zcmb2|=HU3lo{`AFT#{N`qE}K;!r-m#=Xv^+o}cIW6JE^0nUR_|V;IhR&VMY%z`y_i DevlAG literal 0 HcmV?d00001 diff --git a/modules/nf-core/modules/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/faidx/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf new file mode 100644 index 00000000..80cedeab --- /dev/null +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_FAIDX { + tag "$fasta" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + path fasta + + output: + path "*.fai" , emit: fai + path "versions.yml", emit: versions + + script: + """ + samtools faidx $fasta + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml new file mode 100644 index 00000000..16c0b334 --- /dev/null +++ b/modules/nf-core/modules/samtools/faidx/meta.yml @@ -0,0 +1,33 @@ +name: samtools_faidx +description: Index FASTA file +keywords: + - index + - fasta +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - fasta: + type: file + description: FASTA file + pattern: "*.{fa,fasta}" +output: + - fai: + type: file + description: FASTA index file + pattern: "*.{fai}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" + - "@phue" diff --git a/modules/nf-core/modules/samtools/flagstat/functions.nf b/modules/nf-core/modules/samtools/flagstat/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/flagstat/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf new file mode 100644 index 00000000..f9115c6b --- /dev/null +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_FLAGSTAT { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(bam), path(bai) + + output: + tuple val(meta), path("*.flagstat"), emit: flagstat + path "versions.yml" , emit: versions + + script: + """ + samtools flagstat $bam > ${bam}.flagstat + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/flagstat/meta.yml b/modules/nf-core/modules/samtools/flagstat/meta.yml new file mode 100644 index 00000000..9bd9ff89 --- /dev/null +++ b/modules/nf-core/modules/samtools/flagstat/meta.yml @@ -0,0 +1,49 @@ +name: samtools_flagstat +description: Counts the number of alignments in a BAM/CRAM/SAM file for each FLAG type +keywords: + - stats + - mapping + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index for BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - flagstat: + type: file + description: File containing samtools flagstat output + pattern: "*.{flagstat}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" diff --git a/modules/nf-core/modules/samtools/idxstats/functions.nf b/modules/nf-core/modules/samtools/idxstats/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/idxstats/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf new file mode 100644 index 00000000..b005088a --- /dev/null +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_IDXSTATS { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(bam), path(bai) + + output: + tuple val(meta), path("*.idxstats"), emit: idxstats + path "versions.yml" , emit: versions + + script: + """ + samtools idxstats $bam > ${bam}.idxstats + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/idxstats/meta.yml b/modules/nf-core/modules/samtools/idxstats/meta.yml new file mode 100644 index 00000000..ec542f34 --- /dev/null +++ b/modules/nf-core/modules/samtools/idxstats/meta.yml @@ -0,0 +1,50 @@ +name: samtools_idxstats +description: Reports alignment summary statistics for a BAM/CRAM/SAM file +keywords: + - stats + - mapping + - counts + - chromosome + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: Index for BAM/CRAM/SAM file + pattern: "*.{bai,crai,sai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - idxstats: + type: file + description: File containing samtools idxstats output + pattern: "*.{idxstats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" diff --git a/modules/nf-core/modules/samtools/index/functions.nf b/modules/nf-core/modules/samtools/index/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/index/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf new file mode 100644 index 00000000..62254bc8 --- /dev/null +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.crai"), optional:true, emit: crai + tuple val(meta), path("*.csi") , optional:true, emit: csi + path "versions.yml" , emit: versions + + script: + """ + samtools index $options.args $input + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/index/meta.yml b/modules/nf-core/modules/samtools/index/meta.yml new file mode 100644 index 00000000..0905b3cd --- /dev/null +++ b/modules/nf-core/modules/samtools/index/meta.yml @@ -0,0 +1,53 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - crai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/modules/samtools/merge/functions.nf b/modules/nf-core/modules/samtools/merge/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/merge/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf new file mode 100644 index 00000000..fefb423b --- /dev/null +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -0,0 +1,41 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_MERGE { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(input_files) + path fasta + + output: + tuple val(meta), path("${prefix}.bam"), optional:true, emit: bam + tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram + path "versions.yml" , emit: versions + + script: + prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def file_type = input_files[0].getExtension() + def reference = fasta ? "--reference ${fasta}" : "" + """ + samtools merge ${reference} ${prefix}.${file_type} $input_files + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/merge/meta.yml b/modules/nf-core/modules/samtools/merge/meta.yml new file mode 100644 index 00000000..2576a3a3 --- /dev/null +++ b/modules/nf-core/modules/samtools/merge/meta.yml @@ -0,0 +1,54 @@ +name: samtools_merge +description: Merge BAM or CRAM file +keywords: + - merge + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input_files: + type: file + description: BAM/CRAM file + pattern: "*.{bam,cram,sam}" + - fasta: + type: optional file + description: Reference file the CRAM was created with + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.{bam}" + - cram: + type: file + description: CRAM file + pattern: "*.{cram}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@yuukiiwa " + - "@maxulysse" + - "@FriederikeHanssen" diff --git a/modules/nf-core/modules/samtools/sort/functions.nf b/modules/nf-core/modules/samtools/sort/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf new file mode 100644 index 00000000..b30f6f45 --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -0,0 +1,37 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + samtools sort $options.args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/modules/samtools/sort/meta.yml new file mode 100644 index 00000000..3402a068 --- /dev/null +++ b/modules/nf-core/modules/samtools/sort/meta.yml @@ -0,0 +1,44 @@ +name: samtools_sort +description: Sort SAM/BAM/CRAM file +keywords: + - sort + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/modules/samtools/stats/functions.nf b/modules/nf-core/modules/samtools/stats/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/samtools/stats/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf new file mode 100644 index 00000000..aab43410 --- /dev/null +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -0,0 +1,38 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process SAMTOOLS_STATS { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + } else { + container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + } + + input: + tuple val(meta), path(input), path(input_index) + path fasta + + output: + tuple val(meta), path("*.stats"), emit: stats + path "versions.yml" , emit: versions + + script: + def reference = fasta ? "--reference ${fasta}" : "" + """ + samtools stats ${reference} ${input} > ${input}.stats + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/samtools/stats/meta.yml b/modules/nf-core/modules/samtools/stats/meta.yml new file mode 100644 index 00000000..869e62e3 --- /dev/null +++ b/modules/nf-core/modules/samtools/stats/meta.yml @@ -0,0 +1,53 @@ +name: samtools_stats +description: Produces comprehensive statistics from SAM/BAM/CRAM file +keywords: + - statistics + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - fasta: + type: optional file + description: Reference file the CRAM was created with + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@FriederikeHanssen" diff --git a/modules/nf-core/modules/star/align/functions.nf b/modules/nf-core/modules/star/align/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/star/align/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf new file mode 100644 index 00000000..e0ccba8c --- /dev/null +++ b/modules/nf-core/modules/star/align/main.nf @@ -0,0 +1,75 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process STAR_ALIGN { + tag "$meta.id" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + // Note: 2.7X indices incompatible with AWS iGenomes. + conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' + } else { + container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' + } + + input: + tuple val(meta), path(reads) + path index + path gtf + + output: + tuple val(meta), path('*d.out.bam') , emit: bam + tuple val(meta), path('*Log.final.out') , emit: log_final + tuple val(meta), path('*Log.out') , emit: log_out + tuple val(meta), path('*Log.progress.out'), emit: log_progress + path "versions.yml" , emit: versions + + tuple val(meta), path('*sortedByCoord.out.bam') , optional:true, emit: bam_sorted + tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript + tuple val(meta), path('*Aligned.unsort.out.bam') , optional:true, emit: bam_unsorted + tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq + tuple val(meta), path('*.tab') , optional:true, emit: tab + tuple val(meta), path('*.out.junction') , optional:true, emit: junction + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" + def seq_platform = params.seq_platform ? "'PL:$params.seq_platform'" : "" + def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform " + def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' + def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' + """ + STAR \\ + --genomeDir $index \\ + --readFilesIn $reads \\ + --runThreadN $task.cpus \\ + --outFileNamePrefix $prefix. \\ + $out_sam_type \\ + $ignore_gtf \\ + $seq_center \\ + $options.args + + $mv_unsorted_bam + + if [ -f ${prefix}.Unmapped.out.mate1 ]; then + mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq + gzip ${prefix}.unmapped_1.fastq + fi + if [ -f ${prefix}.Unmapped.out.mate2 ]; then + mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq + gzip ${prefix}.unmapped_2.fastq + fi + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml new file mode 100644 index 00000000..2d78b81a --- /dev/null +++ b/modules/nf-core/modules/star/align/meta.yml @@ -0,0 +1,81 @@ +name: star_align +description: Align reads to a reference genome using STAR +keywords: + - align + - fasta + - genome + - reference +tools: + - star: + description: | + STAR is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: https://github.com/alexdobin/STAR + manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf + doi: 10.1093/bioinformatics/bts635 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - index: + type: directory + description: STAR genome index + pattern: "star" +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - log_final: + type: file + description: STAR final log file + pattern: "*Log.final.out" + - log_out: + type: file + description: STAR lot out file + pattern: "*Log.out" + - log_progress: + type: file + description: STAR log progress file + pattern: "*Log.progress.out" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam_sorted: + type: file + description: Sorted BAM file of read alignments (optional) + pattern: "*sortedByCoord.out.bam" + - bam_transcript: + type: file + description: Output BAM file of transcriptome alignment (optional) + pattern: "*toTranscriptome.out.bam" + - bam_unsorted: + type: file + description: Unsorted BAM file of read alignments (optional) + pattern: "*Aligned.unsort.out.bam" + - fastq: + type: file + description: Unmapped FastQ files (optional) + pattern: "*fastq.gz" + - tab: + type: file + description: STAR output tab file(s) (optional) + pattern: "*.tab" + - junction: + type: file + description: STAR chimeric junction output file (optional) + pattern: "*.out.junction" + +authors: + - "@kevinmenden" + - "@drpatelh" + - "@praveenraj2018" diff --git a/modules/nf-core/modules/star/genomegenerate/functions.nf b/modules/nf-core/modules/star/genomegenerate/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/star/genomegenerate/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf new file mode 100644 index 00000000..c932fafe --- /dev/null +++ b/modules/nf-core/modules/star/genomegenerate/main.nf @@ -0,0 +1,76 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process STAR_GENOMEGENERATE { + tag "$fasta" + label 'process_high' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } + + // Note: 2.7X indices incompatible with AWS iGenomes. + conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" + } else { + container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" + } + + input: + path fasta + path gtf + + output: + path "star" , emit: index + path "versions.yml" , emit: versions + + script: + def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' + def args = options.args.tokenize() + if (args.contains('--genomeSAindexNbases')) { + """ + mkdir star + STAR \\ + --runMode genomeGenerate \\ + --genomeDir star/ \\ + --genomeFastaFiles $fasta \\ + --sjdbGTFfile $gtf \\ + --runThreadN $task.cpus \\ + $memory \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//') + END_VERSIONS + """ + } else { + """ + samtools faidx $fasta + NUM_BASES=`gawk '{sum = sum + \$2}END{if ((log(sum)/log(2))/2 - 1 > 14) {printf "%.0f", 14} else {printf "%.0f", (log(sum)/log(2))/2 - 1}}' ${fasta}.fai` + + mkdir star + STAR \\ + --runMode genomeGenerate \\ + --genomeDir star/ \\ + --genomeFastaFiles $fasta \\ + --sjdbGTFfile $gtf \\ + --runThreadN $task.cpus \\ + --genomeSAindexNbases \$NUM_BASES \\ + $memory \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//') + END_VERSIONS + """ + } +} diff --git a/modules/nf-core/modules/star/genomegenerate/meta.yml b/modules/nf-core/modules/star/genomegenerate/meta.yml new file mode 100644 index 00000000..04ade195 --- /dev/null +++ b/modules/nf-core/modules/star/genomegenerate/meta.yml @@ -0,0 +1,37 @@ +name: star_genomegenerate +description: Create index for STAR +keywords: + - index + - fasta + - genome + - reference +tools: + - star: + description: | + STAR is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: https://github.com/alexdobin/STAR + manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf + doi: 10.1093/bioinformatics/bts635 + licence: ['MIT'] +input: + - fasta: + type: file + description: Fasta file of the reference genome + - gtf: + type: file + description: GTF file of the reference genome + +output: + - index: + type: directory + description: Folder containing the star index files + pattern: "star" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@kevinmenden" + - "@drpatelh" diff --git a/modules/nf-core/modules/untar/functions.nf b/modules/nf-core/modules/untar/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/untar/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf new file mode 100644 index 00000000..efb9d825 --- /dev/null +++ b/modules/nf-core/modules/untar/main.nf @@ -0,0 +1,41 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process UNTAR { + tag "$archive" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" + } else { + container "biocontainers/biocontainers:v1.2.0_cv1" + } + + input: + path archive + + output: + path "$untar" , emit: untar + path "versions.yml", emit: versions + + script: + untar = archive.toString() - '.tar.gz' + """ + tar \\ + -xzvf \\ + $options.args \\ + $archive + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml new file mode 100644 index 00000000..51f94995 --- /dev/null +++ b/modules/nf-core/modules/untar/meta.yml @@ -0,0 +1,28 @@ +name: untar +description: Extract files. +keywords: + - untar + - uncompress +tools: + - untar: + description: | + Extract tar.gz files. + documentation: https://www.gnu.org/software/tar/manual/ + licence: ['GPL-3.0-or-later'] +input: + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" +output: + - untar: + type: file + description: + pattern: "*.*" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf new file mode 100644 index 00000000..f9a05d8d --- /dev/null +++ b/subworkflows/local/prepare_genome.nf @@ -0,0 +1,114 @@ +// +// Uncompress and prepare reference genome files +// + +params.genome_options = [:] +params.gffread_options = [:] +params.index_options = [:] +params.star_index_options = [:] + +include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) +include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) +include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams(options: params.gffread_options) +include { GTF2BED } from '../../modules/local/gtf2bed' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) +include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_index_options) + + +workflow PREPARE_GENOME { + take: + prepare_tool_indices + + main: + + // + // Uncompress genome fasta file if required + // + if (params.fasta.endsWith('.gz')) { + ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzip + } else { + ch_fasta = file(params.fasta) + } + + // + // Uncompress GTF annotation file or create from GFF3 if required + // + ch_gffread_version = Channel.empty() + if (params.gtf) { + if (params.gtf.endsWith('.gz')) { + ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip + } else { + ch_gtf = file(params.gtf) + } + } else if (params.gff) { + if (params.gff.endsWith('.gz')) { + ch_gff = GUNZIP_GFF ( params.gff ).gunzip + } else { + ch_gff = file(params.gff) + } + ch_gtf = GFFREAD ( ch_gff ).gtf + ch_gffread_version = GFFREAD.out.version + } + + // + // Uncompress gene BED annotation file or create from GTF if required + // + if (params.gene_bed) { + if (params.gene_bed.endsWith('.gz')) { + ch_gene_bed = GUNZIP_GENE_BED ( params.gene_bed ).gunzip + } else { + ch_gene_bed = file(params.gene_bed) + } + } else { + ch_gene_bed = GTF2BED ( ch_gtf ) + } + + // Index the genome fasta + ch_fasta_fai = Channel.empty() + if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) + else ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai + + // Create dictionary file for the genome fasta + ch_fasta_dict = Channel.empty() + if (params.fasta_dict) ch_fasta_dict = file(params.fasta_dict) + else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict + + // + // Create chromosome sizes file + // + ch_chrom_sizes = GET_CHROM_SIZES ( ch_fasta ).sizes + + // + // Uncompress STAR index or generate from scratch if required + // + ch_star_index = Channel.empty() + ch_star_version = Channel.empty() + if ('star' in prepare_tool_indices) { + if (params.star_index) { + if (params.star_index.endsWith('.tar.gz')) { + ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar + } else { + ch_star_index = file(params.star_index) + } + } else { + ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index + ch_star_version = STAR_GENOMEGENERATE.out.version + } + } + + emit: + fasta = ch_fasta // path: genome.fasta + fai = ch_fasta_fai // path: genome.fasta.fai + dict = ch_fasta_dict // path: genome.fasta.dict + gtf = ch_gtf // path: genome.gtf + gene_bed = ch_gene_bed // path: gene.bed + chrom_sizes = ch_chrom_sizes // path: genome.sizes + star_index = ch_star_index // path: star/index/ + star_version = ch_star_version // path: *.version.txt + gffread_version = ch_gffread_version // path: *.version.txt +} diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf new file mode 100644 index 00000000..85fbd453 --- /dev/null +++ b/subworkflows/nf-core/align_star.nf @@ -0,0 +1,50 @@ +// +// Alignment with STAR +// + +params.align_options = [:] +params.samtools_index_options = [:] +params.samtools_sort_options = [:] +params.samtools_stats_options = [:] +params.seq_platform = [:] + +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: params.seq_platform) +include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) + +workflow ALIGN_STAR { + take: + reads // channel: [ val(meta), [ reads ] ] + index // channel: /path/to/star/index/ + gtf // channel: /path/to/genome.gtf + + main: + + // + // Map reads with STAR + // + STAR_ALIGN ( reads, index, gtf ) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) + + emit: + orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] + log_final = STAR_ALIGN.out.log_final // channel: [ val(meta), log_final ] + log_out = STAR_ALIGN.out.log_out // channel: [ val(meta), log_out ] + log_progress = STAR_ALIGN.out.log_progress // channel: [ val(meta), log_progress ] + bam_sorted = STAR_ALIGN.out.bam_sorted // channel: [ val(meta), bam_sorted ] + bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] + fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] + tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] + star_version = STAR_ALIGN.out.version // path: *.version.txt + + bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] + csi = BAM_SORT_SAMTOOLS.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = BAM_SORT_SAMTOOLS.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf new file mode 100644 index 00000000..ba4d39ef --- /dev/null +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -0,0 +1,45 @@ +// +// Sort, index BAM file and run samtools stats, flagstat and idxstats +// + +params.sort_options = [:] +params.index_options = [:] +params.stats_options = [:] + +include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' addParams( options: params.sort_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) + +workflow BAM_SORT_SAMTOOLS { + take: + ch_bam // channel: [ val(meta), [ bam ] ] + + main: + SAMTOOLS_SORT ( ch_bam ) + SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + + SAMTOOLS_SORT.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai ) + + emit: + bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + version = SAMTOOLS_SORT.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf new file mode 100644 index 00000000..ca05241c --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -0,0 +1,25 @@ +// +// Run SAMtools stats, flagstat and idxstats +// + +params.options = [:] + +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams( options: params.options ) +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' addParams( options: params.options ) +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' addParams( options: params.options ) + +workflow BAM_STATS_SAMTOOLS { + take: + ch_bam_bai // channel: [ val(meta), [ bam ], [bai/csi] ] + + main: + SAMTOOLS_STATS ( ch_bam_bai ) + SAMTOOLS_FLAGSTAT ( ch_bam_bai ) + SAMTOOLS_IDXSTATS ( ch_bam_bai ) + + emit: + stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + version = SAMTOOLS_STATS.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf new file mode 100644 index 00000000..00a05d0d --- /dev/null +++ b/subworkflows/nf-core/markduplicates.nf @@ -0,0 +1,47 @@ +// +// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// + +params.markduplicates_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams(options: params.samtools_stats_options) +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams(options: params.markduplicates_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) + +workflow MARKDUPLICATES { + take: + bam // channel: [ val(meta), [ bam ] ] + + main: + + PICARD_MARKDUPLICATES(bam) + + // + // Index BAM file and run samtools stats, flagstat and idxstats + // + SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) + + PICARD_MARKDUPLICATES.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map{meta, bam, bai, csi -> + if (bai) [meta, bam, bai] + else [meta, bam, csi]} + .set{ch_bam_bai} + + BAM_STATS_SAMTOOLS(ch_bam_bai) + + emit: + bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] + picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt + + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt +} diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf new file mode 100644 index 00000000..9cfccca5 --- /dev/null +++ b/subworkflows/nf-core/recalibrate.nf @@ -0,0 +1,69 @@ +/* +======================================================================================== + RECALIBRATE +======================================================================================== +*/ + +params.applybqsr_options = [:] +params.merge_bam_options = [:] +params.qualimap_bamqc_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/local/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) + +workflow RECALIBRATE { + take: + skip_bamqc // boolean: true/false + skip_samtools // boolean: true/false + bam // channel: [mandatory] bam + dict // channel: [mandatory] dict + fai // channel: [mandatory] fai + fasta // channel: [mandatory] fasta + intervals // channel: [mandatory] intervals + + main: + + bam_recalibrated_index = Channel.empty() + bam_recalibrated = Channel.empty() + bam_reports = Channel.empty() + + bam.combine(intervals).map{ meta, bam, bai, recal, intervals -> + new_meta = meta.clone() + new_meta.id = meta.id + [new_meta, bam, bai, recal, intervals] + }.set{bam_intervals} + + APPLYBQSR(bam_intervals, fasta, fai, dict) + + // STEP 4.5: MERGING AND INDEXING THE RECALIBRATED BAM FILES + if (params.no_intervals) { + bam_recalibrated = APPLYBQSR.out.bam + } else { + APPLYBQSR.out.bam.map{ meta, bam -> + meta.id = meta.id + [meta, bam] + }.groupTuple().set{bam_recalibrated_interval} + + SAMTOOLS_MERGE(bam_recalibrated_interval) + bam_recalibrated = SAMTOOLS_MERGE.out.bam + + SAMTOOLS_INDEX(bam_recalibrated) + bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) + + samtools_stats = Channel.empty() + + if (!skip_samtools) { + SAMTOOLS_STATS(bam_recalibrated_index) + samtools_stats = SAMTOOLS_STATS.out.stats + } + bam_reports = samtools_stats + } + + emit: + bam = bam_recalibrated_index + qc = bam_reports +} diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf new file mode 100644 index 00000000..317944ef --- /dev/null +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -0,0 +1,55 @@ +// +// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// + +params.markduplicates_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] + +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) + +workflow MARK_DUPLICATES_PICARD { + take: + bam // channel: [ val(meta), [ bam ] ] + + main: + + // + // Picard MarkDuplicates + // + PICARD_MARKDUPLICATES ( bam ) + + // + // Index BAM file and run samtools stats, flagstat and idxstats + // + SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + + PICARD_MARKDUPLICATES.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai ) + + emit: + bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] + picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt + + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt +} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index a4e52e39..c3feea1d 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -9,7 +9,6 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowRnavar.initialise(params, log) -// TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } @@ -62,6 +61,72 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/ ======================================================================================== */ +// Check alignment parameters +def prepareToolIndices = [] +prepareToolIndices = params.aligner + +def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] +def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] + +if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false + +modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' + +if (params.save_align_intermeds) { + modules['samtools_sort_genome'].publish_files.put('bam','') + modules['samtools_index_genome'].publish_files.put('bai','') + modules['samtools_index_genome'].publish_files.put('csi','') +} + +if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false + +modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') +if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') + +modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) + +if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) +if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) +if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) +if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) + +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) +include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) +include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) +include { GATK4_SPLITNCIGARREADS } from '../modules/local/gatk4/splitncigarreads/main' addParams(options: modules['gatk_splitncigar_options']) +include { GATK4_VARIANTFILTRATION } from '../modules/local/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) + +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( + genome_options: publish_genome_options, + index_options: publish_index_options, + star_index_options: modules['star_genomegenerate'] +) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( + align_options: modules['star_align'], + samtools_index_options: modules['samtools_index_genome'], + samtools_sort_options: modules['samtools_sort_genome'], + samtools_stats_options: modules['samtools_index_genome'], + seq_platform: params.seq_platform +) +include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( + markduplicates_options: modules['picard_markduplicates'], + samtools_index_options: modules['picard_markduplicates_samtools'], + samtools_stats_options: modules['picard_markduplicates_samtools'] +) +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( + applybqsr_options: modules['applybqsr'], + merge_bam_options: modules['merge_bam_recalibrate'], + samtools_index_options: modules['samtools_index_recalibrate'], + samtools_stats_options: modules['samtools_stats_recalibrate'] +) + + // Info required for completion email and summary def multiqc_report = [] From a3476b95b98a2a264c765f2ac4de2317ac58617c Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 11 Nov 2021 19:14:31 +0100 Subject: [PATCH 044/235] synced all modules --- assets/samplesheet.csv | 3 - assets/samplesheet_test.csv | 2 + bin/gtf2bed | 122 +++++++++++ conf/modules.config | 103 +++++++++- conf/test.config | 19 +- main.nf | 6 +- modules.json | 3 + modules/local/get_chrom_sizes.nf | 7 +- modules/local/gtf_gene_filter.nf | 1 + .../picard/markduplicates/functions.nf | 78 ++++++++ .../modules/picard/markduplicates/main.nf | 52 +++++ .../modules/picard/markduplicates/meta.yml | 52 +++++ nextflow.config | 50 ++++- nextflow_schema.json | 189 ++++++++++++++++++ subworkflows/local/prepare_genome.nf | 20 +- workflows/rnavar.nf | 23 ++- 16 files changed, 704 insertions(+), 26 deletions(-) delete mode 100644 assets/samplesheet.csv create mode 100644 assets/samplesheet_test.csv create mode 100755 bin/gtf2bed create mode 100644 modules/nf-core/modules/picard/markduplicates/functions.nf create mode 100644 modules/nf-core/modules/picard/markduplicates/main.nf create mode 100644 modules/nf-core/modules/picard/markduplicates/meta.yml diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv deleted file mode 100644 index 5f653ab7..00000000 --- a/assets/samplesheet.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, diff --git a/assets/samplesheet_test.csv b/assets/samplesheet_test.csv new file mode 100644 index 00000000..7131bd5d --- /dev/null +++ b/assets/samplesheet_test.csv @@ -0,0 +1,2 @@ +sample,fastq_1,fastq_2,strandedness +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/bin/gtf2bed b/bin/gtf2bed new file mode 100755 index 00000000..4da5c046 --- /dev/null +++ b/bin/gtf2bed @@ -0,0 +1,122 @@ +#!/usr/bin/env perl + +# Copyright (c) 2011 Erik Aronesty (erik@q32.com) +# +# Permission is hereby granted, free of charge, to any person obtaining a copy +# of this software and associated documentation files (the "Software"), to deal +# in the Software without restriction, including without limitation the rights +# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +# copies of the Software, and to permit persons to whom the Software is +# furnished to do so, subject to the following conditions: +# +# The above copyright notice and this permission notice shall be included in +# all copies or substantial portions of the Software. +# +# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +# THE SOFTWARE. +# +# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT. + +use Getopt::Long; + +my $extended; +GetOptions("x"=>\$extended); + +$in = shift @ARGV; + +my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n"; +open IN, $in_cmd; + +while () { + $gff = 2 if /^##gff-version 2/; + $gff = 3 if /^##gff-version 3/; + next if /^#/ && $gff; + + s/\s+$//; + # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr + my @f = split /\t/; + if ($gff) { + # most ver 2's stick gene names in the id field + ($id) = $f[8]=~ /\bID="([^"]+)"/; + # most ver 3's stick unquoted names in the name field + ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3; + } else { + ($id) = $f[8]=~ /transcript_id "([^"]+)"/; + } + + next unless $id && $f[0]; + + if ($f[2] eq 'exon') { + die "no position at exon on line $." if ! $f[3]; + # gff3 puts :\d in exons sometimes + $id =~ s/:\d+$// if $gff == 3; + push @{$exons{$id}}, \@f; + # save lowest start + $trans{$id} = \@f if !$trans{$id}; + } elsif ($f[2] eq 'start_codon') { + #optional, output codon start/stop as "thick" region in bed + $sc{$id}->[0] = $f[3]; + } elsif ($f[2] eq 'stop_codon') { + $sc{$id}->[1] = $f[4]; + } elsif ($f[2] eq 'miRNA' ) { + $trans{$id} = \@f if !$trans{$id}; + push @{$exons{$id}}, \@f; + } +} + +for $id ( + # sort by chr then pos + sort { + $trans{$a}->[0] eq $trans{$b}->[0] ? + $trans{$a}->[3] <=> $trans{$b}->[3] : + $trans{$a}->[0] cmp $trans{$b}->[0] + } (keys(%trans)) ) { + my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}}; + my ($cds, $cde); + ($cds, $cde) = @{$sc{$id}} if $sc{$id}; + + # sort by pos + my @ex = sort { + $a->[3] <=> $b->[3] + } @{$exons{$id}}; + + my $beg = $ex[0][3]; + my $end = $ex[-1][4]; + + if ($dir eq '-') { + # swap + $tmp=$cds; + $cds=$cde; + $cde=$tmp; + $cds -= 2 if $cds; + $cde += 2 if $cde; + } + + # not specified, just use exons + $cds = $beg if !$cds; + $cde = $end if !$cde; + + # adjust start for bed + --$beg; --$cds; + + my $exn = @ex; # exon count + my $exst = join ",", map {$_->[3]-$beg-1} @ex; # exon start + my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex; # exon size + + my $gene_id; + my $extend = ""; + if ($extended) { + ($gene_id) = $attr =~ /gene_name "([^"]+)"/; + ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id; + $extend="\t$gene_id"; + } + # added an extra comma to make it look exactly like ucsc's beds + print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n"; +} + +close IN; diff --git a/conf/modules.config b/conf/modules.config index 0b1bfdec..865c1b44 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,11 +22,110 @@ params { modules { + 'cat_fastq' { + publish_dir = 'fastq' + } + 'star_genomegenerate' { + args = "--sjdbOverhang ${params.read_length - 1}" + publish_dir = 'genome/index' + } + 'star_align' { + args = "--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" + publish_files = false + } + 'samtools_sort_genome' { + suffix = '.sorted' + publish_files = false + } + 'samtools_index_genome' { + args = '' + suffix = '.sorted' + publish_files = false + } + 'picard_markduplicates' { + args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' + suffix = '.markdup.sorted' + publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] + publish_dir = "preprocessing" + publish_by_meta = true + } + 'picard_markduplicates_samtools' { + args = '' + suffix = '.markdup.sorted' + publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "preprocessing" + publish_by_meta = true + } + 'gatk_splitncigar_options' { + args = '' + suffix = '.split_cigar' + publish_files = false + } + 'gatk_baserecalibrator' { + args = '' + suffix = '' + publish_files = false + } + 'gatk_applybqsr' { + args = '' + suffix = '.markdup.recalibrated' + publish_files = false + } + 'gatk_haplotypecaller' { + args = '-dont-use-soft-clipped-bases --standard-min-confidence-threshold-for-calling 20' + suffix = '' + publish_files = false + } + 'gatk_intervallisttools' { + args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' + suffix = '' + publish_files = false + } + 'gatk_bedtointervallist' { + args = '' + publish_files = false + } + // RECALIBRATE + 'applybqsr' { + suffix = '.recal' + publish_files = false + } + 'merge_bam_recalibrate' { + suffix = '.recal.merge' + publish_by_meta = true + publish_files = ['bam':'', 'bai':''] + publish_dir = 'preprocessing' + } + 'samtools_index_recalibrate' { + suffix = 'recalibrated' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['bam':'', 'bai':''] + } + 'samtools_stats_recalibrate' { + publish_by_meta = true + publish_dir = 'reports/samtools_stats' + } + 'gatk_mergevcfs' { + suffix = '.haplotypecaller' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] + } + 'gatk_variantfilter' { + args = '' + suffix = '.filtered' + publish_by_meta = true + publish_dir = 'variant_filtering' + publish_files = ['vcf':'', 'vcf.idx':''] + } 'fastqc' { - args = "--quiet" + args = "--quiet" + publish_files = false } 'multiqc' { - args = "" + args = "" + publish_dir = 'reports' } } } diff --git a/conf/test.config b/conf/test.config index c69c8094..c56b48c1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,10 +20,21 @@ params { max_time = 6.h // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = 'assets/samplesheet_test.csv' // Genome references - genome = 'R64-1-1' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' + fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' + fasta_dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' + gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' + //gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' + + // Known genome resources (optional) + dbsnp_vcf = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' + dbsnp_vcf_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' + known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' + known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' + + // STAR index (optional) + star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' } diff --git a/main.nf b/main.nf index 37839193..b80e3871 100644 --- a/main.nf +++ b/main.nf @@ -17,7 +17,11 @@ nextflow.enable.dsl = 2 ======================================================================================== */ -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') +params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') +params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') +params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') /* ======================================================================================== diff --git a/modules.json b/modules.json index d91398e0..a24edf56 100644 --- a/modules.json +++ b/modules.json @@ -33,6 +33,9 @@ "multiqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, + "picard/markduplicates": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, "samtools/faidx": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf index b9fa2ac9..95957db1 100644 --- a/modules/local/get_chrom_sizes.nf +++ b/modules/local/get_chrom_sizes.nf @@ -5,15 +5,16 @@ params.options = [:] process GET_CHROM_SIZES { tag "$fasta" + label "process_medium" publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::samtools=1.10" : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.10--h9402c20_2" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.10--h9402c20_2" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf index 79088854..07138ba7 100644 --- a/modules/local/gtf_gene_filter.nf +++ b/modules/local/gtf_gene_filter.nf @@ -5,6 +5,7 @@ params.options = [:] process GTF_GENE_FILTER { tag "$fasta" + label "process_medium" publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } diff --git a/modules/nf-core/modules/picard/markduplicates/functions.nf b/modules/nf-core/modules/picard/markduplicates/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/picard/markduplicates/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf new file mode 100644 index 00000000..37b825d7 --- /dev/null +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -0,0 +1,52 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process PICARD_MARKDUPLICATES { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::picard=2.25.7' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/picard:2.25.7--hdfd78af_0" + } else { + container "quay.io/biocontainers/picard:2.25.7--hdfd78af_0" + } + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam") , emit: bam + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.metrics.txt"), emit: metrics + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + picard \\ + -Xmx${avail_mem}g \\ + MarkDuplicates \\ + $options.args \\ + -I $bam \\ + -O ${prefix}.bam \\ + -M ${prefix}.MarkDuplicates.metrics.txt + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/picard/markduplicates/meta.yml b/modules/nf-core/modules/picard/markduplicates/meta.yml new file mode 100644 index 00000000..c9a08b36 --- /dev/null +++ b/modules/nf-core/modules/picard/markduplicates/meta.yml @@ -0,0 +1,52 @@ +name: picard_markduplicates +description: Locate and tag duplicate reads in a BAM file +keywords: + - markduplicates + - pcr + - duplicates + - bam + - sam + - cram +tools: + - picard: + description: | + A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) + data and formats such as SAM/BAM/CRAM and VCF. + homepage: https://broadinstitute.github.io/picard/ + documentation: https://broadinstitute.github.io/picard/ + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file + pattern: "*.{bam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM file with duplicate reads marked/removed + pattern: "*.{bam}" + - bai: + type: file + description: An optional BAM index file. If desired, --CREATE_INDEX must be passed as a flag + pattern: "*.{bai}" + - metrics: + type: file + description: Duplicate metrics file generated by picard + pattern: "*.{metrics.txt}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@projectoriented" diff --git a/nextflow.config b/nextflow.config index 439746a0..3d8c5c44 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,14 +9,56 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags - // Input options - input = null + // Pipeline options - // References + // Mandatory option + input = null // sample sheet + + // Genome and reference options genome = null igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_ignore = false + save_reference = false + save_merged_fastq = false + gtf = null + + // Sequence read information + read_length = 150 // Required for STAR to build index and align reads + + // Alignment + aligner = 'star' // Only STAR is currently supported. + star_index = null // Provide STAR index if there is already a pre-defined one, or set to null to create one by the pipeline + star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR + seq_center = null + seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work + bam_csi_index = false + save_unaligned = false + save_align_intermeds = false + + //Known variant references + known_indels = null + known_indels_index = null + dbsnp_vcf = null + dbsnp_vcf_index = null + + // Variant calling + no_intervals = false + + // Skip steps + skip_intervallisttools = false + skip_variantfiltration = false + + // GATK intervallist parameters + scatter_count = 6 + + //GATK variant filter parameters + window = 35 + cluster = 3 + fs_filter = 30.0 + qd_filter = 2.0 + + // QC + skip_qc = false // MultiQC options multiqc_config = null diff --git a/nextflow_schema.json b/nextflow_schema.json index d3eb24a9..55aa4d2f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -41,6 +41,10 @@ "type": "string", "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", "fa_icon": "fas fa-file-signature" + }, + "save_merged_fastq": { + "type": "string", + "description": "Save FastQ files after merging re-sequenced libraries in the results directory." } } }, @@ -65,6 +69,18 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, + "fasta_dict": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to FASTA dictionary file.", + "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference." + }, + "fasta_fai": { + "type": "string", + "fa_icon": "fas fa-file", + "help_text": "> **NB** If none provided, will be generated automatically from the FASTA reference", + "description": "Path to FASTA reference index." + }, "igenomes_base": { "type": "string", "format": "directory-path", @@ -79,6 +95,163 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "gtf": { + "type": "string", + "description": "Path to GTF annotation file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified." + }, + "gff": { + "type": "string", + "description": "Path to GFF3 annotation file.", + "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." + }, + "gene_bed": { + "type": "string", + "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." + }, + "read_length": { + "type": "number", + "default": 150, + "description": "Read length", + "help_text": "Specify the read length for the STAR aligner." + }, + "save_reference": { + "type": "string", + "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", + "description": "If generated by the pipeline, save the STAR index in the results directory." + }, + "known_indels": { + "type": "string", + "description": "Path to known indels VCF file" + }, + "known_indels_index": { + "type": "string", + "description": "Path to known indels index file" + }, + "dbsnp_vcf": { + "type": "string", + "description": "Path to dbSNP VCF file" + }, + "dbsnp_vcf_index": { + "type": "string", + "description": "Path to dbSNP VCF index file" + } + } + }, + "alignment_options": { + "title": "Alignment options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters related to read alignment", + "properties": { + "aligner": { + "type": "string", + "default": "star", + "description": "Specifies the alignment algorithm to use. Currently available option is 'star'", + "help_text": "This parameter define which aligner is to be used for aligning the RNA reads to the reference genome. Currently only STAR aligner is supported. So use 'star' as the value for this option." + }, + "star_index": { + "type": "string", + "description": "Path to STAR index folder or compressed file (tar.gz)", + "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." + }, + "star_ignore_sjdbgtf": { + "type": "string", + "description": "Do not use GTF file during STAR index buidling step", + "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." + }, + "seq_center": { + "type": "string", + "description": "Sequencing center information to be added to read group of BAM files.", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " + }, + "seq_platform": { + "type": "string", + "default": "illumina", + "description": "Specify the sequencing platform used", + "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " + }, + "save_unaligned": { + "type": "string", + "description": "Where possible, save unaligned reads from aligner to the results directory.", + "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." + }, + "save_align_intermeds": { + "type": "string", + "description": "Save the intermediate BAM files from the alignment step.", + "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." + }, + "bam_csi_index": { + "type": "boolean", + "description": "Create a CSI index for BAM files instead of the traditional BAI index. This will be required for genomes with larger chromosome sizes." + } + } + }, + "pipeline_stage_options": { + "title": "Pipeline stage options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters that control the stages in the pipeline", + "properties": { + "skip_intervallisttools": { + "type": "string", + "description": "Skip the process of preparing interval lists for the GATK variant calling step", + "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." + }, + "skip_variantfiltration": { + "type": "string", + "description": "Skip variant filtering of GATK", + "help_text": "Set this parameter if you don't want to filter any variants. " + }, + "skip_qc": { + "type": "string", + "description": "Skip QC reports", + "help_text": "This parameter disable all QC reports from recalibrated BAM file." + } + } + }, + "tool_parameters": { + "title": "Tool parameters", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define parameters of the tools used in the pipeline", + "properties": { + "scatter_count": { + "type": "integer", + "default": 6, + "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", + "help_text": "Set this parameter to decide the number of splits for the gene interval list file." + }, + "no_intervals": { + "type": "string", + "description": "Do not use gene interval file during variant calling", + "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", + "default": "False" + }, + "window": { + "type": "integer", + "default": 35, + "description": "The window size (in bases) in which to evaluate clustered SNPs.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." + }, + "cluster": { + "type": "integer", + "default": 3, + "description": "The number of SNPs which make up a cluster. Must be at least 2.", + "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." + }, + "fs_filter": { + "type": "number", + "default": 30, + "description": "Value to be used for the FisherStrand (FS) filter", + "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" + }, + "qd_filter": { + "type": "number", + "default": 2, + "description": "Value to be used for the QualByDepth (QD) filter", + "help_text": "This parameter defines the value to use for the QualByDepth (QD) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 2.0" } } }, @@ -281,5 +454,21 @@ { "$ref": "#/definitions/generic_options" } + , + { + "$ref": "#/definitions/tool_parameters" + } + , + { + "$ref": "#/definitions/pipeline_stage_options" + } + , + { + "$ref": "#/definitions/alignment_options" + } + ], + "required": [ + "aligner", + "seq_platform" ] } diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index f9a05d8d..7baea627 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -6,6 +6,7 @@ params.genome_options = [:] params.gffread_options = [:] params.index_options = [:] params.star_index_options = [:] +params.star_untar_options = [:] include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) @@ -17,7 +18,7 @@ include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/module include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) -include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_index_options) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_untar_options) workflow PREPARE_GENOME { @@ -26,11 +27,14 @@ workflow PREPARE_GENOME { main: + ch_versions = Channel.empty() + // // Uncompress genome fasta file if required // if (params.fasta.endsWith('.gz')) { ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzip + ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { ch_fasta = file(params.fasta) } @@ -42,17 +46,19 @@ workflow PREPARE_GENOME { if (params.gtf) { if (params.gtf.endsWith('.gz')) { ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip + ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { ch_gtf = file(params.gtf) } } else if (params.gff) { if (params.gff.endsWith('.gz')) { ch_gff = GUNZIP_GFF ( params.gff ).gunzip + ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions) } else { ch_gff = file(params.gff) } ch_gtf = GFFREAD ( ch_gff ).gtf - ch_gffread_version = GFFREAD.out.version + ch_versions = ch_versions.mix(GFFREAD.out.versions) } // @@ -61,11 +67,13 @@ workflow PREPARE_GENOME { if (params.gene_bed) { if (params.gene_bed.endsWith('.gz')) { ch_gene_bed = GUNZIP_GENE_BED ( params.gene_bed ).gunzip + ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { ch_gene_bed = file(params.gene_bed) } } else { - ch_gene_bed = GTF2BED ( ch_gtf ) + ch_gene_bed = GTF2BED ( ch_gtf ).bed + ch_versions = ch_versions.mix(GTF2BED.out.versions) } // Index the genome fasta @@ -92,12 +100,13 @@ workflow PREPARE_GENOME { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar + ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { ch_star_index = file(params.star_index) } } else { ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index - ch_star_version = STAR_GENOMEGENERATE.out.version + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) } } @@ -109,6 +118,5 @@ workflow PREPARE_GENOME { gene_bed = ch_gene_bed // path: gene.bed chrom_sizes = ch_chrom_sizes // path: genome.sizes star_index = ch_star_index // path: star/index/ - star_version = ch_star_version // path: *.version.txt - gffread_version = ch_gffread_version // path: *.version.txt + versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index c3feea1d..ec85559a 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -10,12 +10,24 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) WorkflowRnavar.initialise(params, log) // Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } +// Check input path parameters to see if they exist +def checkPathParamList = [ + params.input, + params.fasta, + params.gtf, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + params.known_indels, + params.known_indels_index, + params.star_index] + +for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } +if(!params.star_index and !params.gtf){ exit 1, "GTF file is required to build a STAR reference index! Use option -gtf to provide a GTF file." } + /* ======================================================================================== CONFIG FILES @@ -126,7 +138,6 @@ include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' samtools_stats_options: modules['samtools_stats_recalibrate'] ) - // Info required for completion email and summary def multiqc_report = [] @@ -134,6 +145,12 @@ workflow RNAVAR { ch_versions = Channel.empty() + // + // SUBWORKFLOW: Uncompress and prepare reference genome files + // + PREPARE_GENOME (prepareToolIndices) + ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) + // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // From b6958a8083e6e18b3bc454b545ccb0f6588971d9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 16 Nov 2021 15:32:31 +0100 Subject: [PATCH 045/235] fix: issues with splitNCigarReads --- modules.json | 3 + .../gatk4/splitncigarreads/functions.nf | 78 +++++++++++++ .../modules/gatk4/splitncigarreads/main.nf | 45 ++++++++ .../modules/gatk4/splitncigarreads/meta.yml | 41 +++++++ .../modules/picard/markduplicates/main.nf | 6 +- subworkflows/nf-core/align_star.nf | 9 +- subworkflows/nf-core/bam_sort_samtools.nf | 9 +- subworkflows/nf-core/bam_stats_samtools.nf | 12 +- subworkflows/nf-core/markduplicates.nf | 8 +- subworkflows/nf-core/splitn_cigar_reads.nf | 43 ++++--- workflows/rnavar.nf | 109 +++++++++++++++++- 11 files changed, 328 insertions(+), 35 deletions(-) create mode 100644 modules/nf-core/modules/gatk4/splitncigarreads/functions.nf create mode 100644 modules/nf-core/modules/gatk4/splitncigarreads/main.nf create mode 100644 modules/nf-core/modules/gatk4/splitncigarreads/meta.yml diff --git a/modules.json b/modules.json index a24edf56..508f1458 100644 --- a/modules.json +++ b/modules.json @@ -24,6 +24,9 @@ "gatk4/intervallisttools": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, + "gatk4/splitncigarreads": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, "gffread": { "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" }, diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/functions.nf b/modules/nf-core/modules/gatk4/splitncigarreads/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/splitncigarreads/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf new file mode 100644 index 00000000..a044b1a9 --- /dev/null +++ b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf @@ -0,0 +1,45 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_SPLITNCIGARREADS { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + tuple val(meta), path(bam) + path(fasta) + path(fai) + path(dict) + + output: + tuple val(meta), path('*.bam'), emit: bam + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + gatk SplitNCigarReads \\ + -R $fasta \\ + -I $bam \\ + -O ${prefix}.bam \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml new file mode 100644 index 00000000..f287ede4 --- /dev/null +++ b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml @@ -0,0 +1,41 @@ +name: gatk4_splitncigarreads +description: Splits reads that contain Ns in their cigar string +keywords: + - vcf + - merge +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test'] + - bam: + type: list + description: BAM/SAM/CRAM file containing reads + pattern: "*.{bam,sam,cram}" + - fasta: + type: tuple of files + description: | + Tuple of fasta file (first), sequence dict (second) and fasta index (third) + pattern: ["*.fasta", "*.dict", "*.fai"] +output: + - bam: + type: file + description: Output file with split reads (BAM/SAM/CRAM) + pattern: "*.{bam,sam,cram}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@kevinmenden" diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index 37b825d7..b34e3113 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -40,9 +40,9 @@ process PICARD_MARKDUPLICATES { -Xmx${avail_mem}g \\ MarkDuplicates \\ $options.args \\ - -I $bam \\ - -O ${prefix}.bam \\ - -M ${prefix}.MarkDuplicates.metrics.txt + INPUT=$bam \\ + OUTPUT=${prefix}.bam \\ + METRICS_FILE=${prefix}.MarkDuplicates.metrics.txt cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index 85fbd453..fbe97292 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -19,15 +19,19 @@ workflow ALIGN_STAR { main: + ch_versions = Channel.empty() + // // Map reads with STAR // STAR_ALIGN ( reads, index, gtf ) + ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first()) // // Sort, index BAM file and run samtools stats, flagstat and idxstats // BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) + ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions.first()) emit: orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] @@ -38,7 +42,6 @@ workflow ALIGN_STAR { bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] - star_version = STAR_ALIGN.out.version // path: *.version.txt bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] @@ -46,5 +49,7 @@ workflow ALIGN_STAR { stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = BAM_SORT_SAMTOOLS.out.version // path: *.version.txt + + versions = ch_versions // channel: [ versions.yml ] + } diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index ba4d39ef..d52ec8ab 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -15,8 +15,14 @@ workflow BAM_SORT_SAMTOOLS { ch_bam // channel: [ val(meta), [ bam ] ] main: + + ch_versions = Channel.empty() + SAMTOOLS_SORT ( ch_bam ) + ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) + SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) SAMTOOLS_SORT.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) @@ -32,6 +38,7 @@ workflow BAM_SORT_SAMTOOLS { .set { ch_bam_bai } BAM_STATS_SAMTOOLS ( ch_bam_bai ) + ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) emit: bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] @@ -41,5 +48,5 @@ workflow BAM_SORT_SAMTOOLS { stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - version = SAMTOOLS_SORT.out.version // path: *.version.txt + versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index ca05241c..b80482e3 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -13,13 +13,21 @@ workflow BAM_STATS_SAMTOOLS { ch_bam_bai // channel: [ val(meta), [ bam ], [bai/csi] ] main: - SAMTOOLS_STATS ( ch_bam_bai ) + ch_versions = Channel.empty() + + SAMTOOLS_STATS ( ch_bam_bai, [] ) + ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) + SAMTOOLS_FLAGSTAT ( ch_bam_bai ) + ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions.first()) + SAMTOOLS_IDXSTATS ( ch_bam_bai ) + ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions.first()) emit: stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), [ stats ] ] flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ] idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - version = SAMTOOLS_STATS.out.version // path: *.version.txt + + versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index 00a05d0d..05579425 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -16,12 +16,16 @@ workflow MARKDUPLICATES { main: + ch_versions = Channel.empty() + PICARD_MARKDUPLICATES(bam) + ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) // // Index BAM file and run samtools stats, flagstat and idxstats // SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) PICARD_MARKDUPLICATES.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) @@ -32,16 +36,16 @@ workflow MARKDUPLICATES { .set{ch_bam_bai} BAM_STATS_SAMTOOLS(ch_bam_bai) + ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) emit: bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] - picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt + versions = ch_versions // channel: [versions.yml] } diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf index 317944ef..3adf6b63 100644 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -1,32 +1,38 @@ // -// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// GATK4 SplitNCigarReads // -params.markduplicates_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] +params.gatk_splitncigar_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams( options: params.markduplicates_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.samtools_stats_options ) +include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' addParams( options: params.gatk_splitncigar_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) -workflow MARK_DUPLICATES_PICARD { +workflow GATK4_SPLITNCIGAR { take: - bam // channel: [ val(meta), [ bam ] ] + bam // channel: [ val(meta), [ bam ] ] + fasta // channel: [ fasta ] + fasta_fai // channel: [ fai ] + fasta_dict // channel: [ dict ] main: + ch_versions = Channel.empty() + // - // Picard MarkDuplicates + // GATK4 // - PICARD_MARKDUPLICATES ( bam ) + GATK4_SPLITNCIGARREADS ( bam, fasta, fasta_fai, fasta_dict) + ch_versions = ch_versions.mix(GATK4_SPLITNCIGARREADS.out.versions.first()) // // Index BAM file and run samtools stats, flagstat and idxstats // - SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - PICARD_MARKDUPLICATES.out.bam + GATK4_SPLITNCIGARREADS.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) .map { @@ -39,17 +45,10 @@ workflow MARK_DUPLICATES_PICARD { } .set { ch_bam_bai } - BAM_STATS_SAMTOOLS ( ch_bam_bai ) emit: - bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] - metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] - picard_version = PICARD_MARKDUPLICATES.out.version // path: *.version.txt - + bam = GATK4_SPLITNCIGARREADS.out.bam // channel: [ val(meta), [ bam ] ] bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - samtools_version = SAMTOOLS_INDEX.out.version // path: *.version.txt + versions = ch_versions // channel: [ versions.yml ] } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index ec85559a..6a501e1c 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -110,7 +110,6 @@ include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedto include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) -include { GATK4_SPLITNCIGARREADS } from '../modules/local/gatk4/splitncigarreads/main' addParams(options: modules['gatk_splitncigar_options']) include { GATK4_VARIANTFILTRATION } from '../modules/local/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) @@ -131,6 +130,10 @@ include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates samtools_index_options: modules['picard_markduplicates_samtools'], samtools_stats_options: modules['picard_markduplicates_samtools'] ) +include { GATK4_SPLITNCIGAR } from '../subworkflows/nf-core/splitn_cigar_reads' addParams( + gatk_splitncigar_options: modules['gatk_splitncigar_options'], + samtools_index_options: modules['picard_markduplicates_samtools'] +) include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( applybqsr_options: modules['applybqsr'], merge_bam_options: modules['merge_bam_recalibrate'], @@ -145,10 +148,11 @@ workflow RNAVAR { ch_versions = Channel.empty() - // + // // SUBWORKFLOW: Uncompress and prepare reference genome files // PREPARE_GENOME (prepareToolIndices) + ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) // @@ -157,16 +161,115 @@ workflow RNAVAR { INPUT_CHECK ( ch_input ) + .reads + .map { + meta, fastq -> + meta.id = meta.id.split('_')[0..-2].join('_') + [ meta, fastq ] } + .groupTuple(by: [0]) + .branch { + meta, fastq -> + single : fastq.size() == 1 + return [ meta, fastq.flatten() ] + multiple: fastq.size() > 1 + return [ meta, fastq.flatten() ] + } + .set { ch_fastq } ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + // + // MODULE: Concatenate FastQ files from same sample if required + // + CAT_FASTQ ( + ch_fastq.multiple + ) + .reads + .mix(ch_fastq.single) + .set { ch_cat_fastq } + ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null)) + // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_cat_fastq ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // PREPARE THE INTERVAL LIST FROM GTF FILE + ch_interval_list = Channel.empty() + GATK4_BEDTOINTERVALLIST(ch_genome_bed, PREPARE_GENOME.out.dict) + ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list + ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions.first().ifEmpty(null)) + + // + // SUBWORKFLOW: Alignment with STAR + // + ch_genome_bam = Channel.empty() + ch_genome_bam_index = Channel.empty() + ch_samtools_stats = Channel.empty() + ch_samtools_flagstat = Channel.empty() + ch_samtools_idxstats = Channel.empty() + ch_star_multiqc = Channel.empty() + ch_aligner_pca_multiqc = Channel.empty() + ch_aligner_clustering_multiqc = Channel.empty() + + if (params.aligner == 'star') { + ALIGN_STAR ( + ch_cat_fastq, + PREPARE_GENOME.out.star_index, + PREPARE_GENOME.out.gtf + ) + ch_genome_bam = ALIGN_STAR.out.bam + ch_genome_bam_index = ALIGN_STAR.out.bai + ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript + ch_samtools_stats = ALIGN_STAR.out.stats + ch_samtools_flagstat = ALIGN_STAR.out.flagstat + ch_samtools_idxstats = ALIGN_STAR.out.idxstats + ch_star_multiqc = ALIGN_STAR.out.log_final + if (params.bam_csi_index) ch_genome_bam_index = ALIGN_STAR.out.csi + ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) + + // SUBWORKFLOW: Mark duplicates with Picard + MARKDUPLICATES(ch_genome_bam) + ch_genome_bam = MARKDUPLICATES.out.bam + ch_genome_bam_index = MARKDUPLICATES.out.bai + ch_samtools_stats = MARKDUPLICATES.out.stats + ch_samtools_flagstat = MARKDUPLICATES.out.flagstat + ch_samtools_idxstats = MARKDUPLICATES.out.idxstats + ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics + if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi + ch_versions = ch_versions.mix(MARKDUPLICATES.out.versions.first().ifEmpty(null)) + + // MODULE: SplitNCigarReads from GATK4 + // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). + bam_splitncigar = Channel.empty() + GATK4_SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) + bam_splitncigar = GATK4_SPLITNCIGAR.out.bam + ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first().ifEmpty(null)) + + ch_bqsr_table = Channel.empty() + + // MODULE: BaseRecalibrator from GATK4 + known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() + known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() + + ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() + + GATK4_BASERECALIBRATOR( + bam_splitncigar, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list_baserecalibrator, + known_sites, + known_sites_tbi + ) + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + + + } + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From cad30e8c4f7fb562bb752cc1ec3c7f51ab1c0b0f Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 16 Nov 2021 16:18:12 +0100 Subject: [PATCH 046/235] fix: replaced local gatk4 modules with nfcore repo --- modules.json | 3 + .../modules/gatk4/applybqsr/functions.nf | 78 +++++++++++++++++++ .../nf-core/modules/gatk4/applybqsr/main.nf | 55 +++++++++++++ .../nf-core/modules/gatk4/applybqsr/meta.yml | 67 ++++++++++++++++ nextflow_schema.json | 2 +- subworkflows/nf-core/recalibrate.nf | 42 +++++----- workflows/rnavar.nf | 28 ++++++- 7 files changed, 251 insertions(+), 24 deletions(-) create mode 100644 modules/nf-core/modules/gatk4/applybqsr/functions.nf create mode 100644 modules/nf-core/modules/gatk4/applybqsr/main.nf create mode 100644 modules/nf-core/modules/gatk4/applybqsr/meta.yml diff --git a/modules.json b/modules.json index 508f1458..5b2e7d52 100644 --- a/modules.json +++ b/modules.json @@ -12,6 +12,9 @@ "fastqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, + "gatk4/applybqsr": { + "git_sha": "34268347447cd60013b69279a42aa9d081592735" + }, "gatk4/baserecalibrator": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, diff --git a/modules/nf-core/modules/gatk4/applybqsr/functions.nf b/modules/nf-core/modules/gatk4/applybqsr/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/applybqsr/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf new file mode 100644 index 00000000..c89a4a4d --- /dev/null +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -0,0 +1,55 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process GATK4_APPLYBQSR { + tag "$meta.id" + label 'process_low' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" + } else { + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" + } + + input: + tuple val(meta), path(input), path(input_index), path(bqsr_table) + path fasta + path fai + path dict + path intervals + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def interval = intervals ? "-L ${intervals}" : "" + if (!task.memory) { + log.info '[GATK ApplyBQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk ApplyBQSR \\ + -R $fasta \\ + -I $input \\ + --bqsr-recal-file $bqsr_table \\ + $interval \\ + --tmp-dir . \\ + -O ${prefix}.bam \\ + $options.args + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/applybqsr/meta.yml b/modules/nf-core/modules/gatk4/applybqsr/meta.yml new file mode 100644 index 00000000..4e3b2f9a --- /dev/null +++ b/modules/nf-core/modules/gatk4/applybqsr/meta.yml @@ -0,0 +1,67 @@ +name: gatk4_applybqsr +description: Apply base quality score recalibration (BQSR) to a bam file +keywords: + - bqsr + - bam +tools: + - gatk4: + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - bqsr_table: + type: file + description: Recalibration table from gatk4_baserecalibrator + - fasta: + type: file + description: The reference fasta file + pattern: "*.fasta" + - fai: + type: file + description: Index of reference fasta file + pattern: "*.fasta.fai" + - dict: + type: file + description: GATK sequence dictionary + pattern: "*.dict" + - intervalsBed: + type: file + description: Bed file with the genomic regions included in the library (optional) + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam: + type: file + description: Recalibrated BAM file + pattern: "*.{bam}" + +authors: + - "@yocra3" + - "@FriederikeHanssen" diff --git a/nextflow_schema.json b/nextflow_schema.json index 55aa4d2f..945d4f88 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -224,7 +224,7 @@ "help_text": "Set this parameter to decide the number of splits for the gene interval list file." }, "no_intervals": { - "type": "string", + "type": "boolean", "description": "Do not use gene interval file during variant calling", "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", "default": "False" diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 9cfccca5..e7f1635d 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -10,7 +10,7 @@ params.qualimap_bamqc_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/local/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) @@ -27,17 +27,14 @@ workflow RECALIBRATE { main: + ch_versions = Channel.empty() + bam_recalibrated_index = Channel.empty() bam_recalibrated = Channel.empty() bam_reports = Channel.empty() - bam.combine(intervals).map{ meta, bam, bai, recal, intervals -> - new_meta = meta.clone() - new_meta.id = meta.id - [new_meta, bam, bai, recal, intervals] - }.set{bam_intervals} - - APPLYBQSR(bam_intervals, fasta, fai, dict) + APPLYBQSR(bam, fasta, fai, dict, intervals) + ch_versions = ch_versions.mix(APPLYBQSR.out.versions.first()) // STEP 4.5: MERGING AND INDEXING THE RECALIBRATED BAM FILES if (params.no_intervals) { @@ -48,22 +45,29 @@ workflow RECALIBRATE { [meta, bam] }.groupTuple().set{bam_recalibrated_interval} - SAMTOOLS_MERGE(bam_recalibrated_interval) + SAMTOOLS_MERGE(bam_recalibrated_interval, fasta) bam_recalibrated = SAMTOOLS_MERGE.out.bam + ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) + } - SAMTOOLS_INDEX(bam_recalibrated) - bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) + SAMTOOLS_INDEX(bam_recalibrated) + bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - samtools_stats = Channel.empty() + samtools_stats = Channel.empty() - if (!skip_samtools) { - SAMTOOLS_STATS(bam_recalibrated_index) - samtools_stats = SAMTOOLS_STATS.out.stats - } - bam_reports = samtools_stats + if (!skip_samtools) { + SAMTOOLS_STATS(bam_recalibrated_index, []) + samtools_stats = SAMTOOLS_STATS.out.stats + ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) } + bam_reports = samtools_stats + emit: - bam = bam_recalibrated_index - qc = bam_reports + bam = bam_recalibrated_index + qc = bam_reports + + versions = ch_versions + } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 6a501e1c..c930aaa4 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -245,12 +245,11 @@ workflow RNAVAR { // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() GATK4_SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) - bam_splitncigar = GATK4_SPLITNCIGAR.out.bam + bam_splitncigar = GATK4_SPLITNCIGAR.out.bam.join(GATK4_SPLITNCIGAR.out.bai, by: [0]) ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first().ifEmpty(null)) - ch_bqsr_table = Channel.empty() - // MODULE: BaseRecalibrator from GATK4 + ch_bqsr_table = Channel.empty() known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() @@ -265,8 +264,29 @@ workflow RNAVAR { known_sites, known_sites_tbi ) - ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) + + // MODULE: ApplyBaseRecalibrator from GATK4 + bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) + bam_recalibrated = Channel.empty() + bam_recalibrated_qc = Channel.empty() + + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() + + RECALIBRATE( + ('bamqc' in params.skip_qc), + ('samtools' in params.skip_qc), + bam_applybqsr, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.fasta, + ch_interval_list_applybqsr + ) + bam_recalibrated = RECALIBRATE.out.bam + bam_recalibrated_qc = RECALIBRATE.out.qc + ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) } From 185d6d8754de937a65ea7040508591415d3996c6 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 18 Nov 2021 11:30:46 +0100 Subject: [PATCH 047/235] fixes; code cleanup; replaced local gatk4 modules with repo modules; testing --- assets/samplesheet_full.csv | 5 + assets/samplesheet_test.csv | 2 + bin/check_samplesheet.py | 107 ++++++++++++------ conf/modules.config | 9 +- conf/test.config | 3 +- modules.json | 9 ++ modules/local/gatk4/applybqsr/functions.nf | 68 ----------- modules/local/gatk4/applybqsr/main.nf | 46 -------- modules/local/gatk4/applybqsr/meta.yml | 58 ---------- .../local/gatk4/haplotypecaller/functions.nf | 54 +++++---- modules/local/gatk4/haplotypecaller/main.nf | 38 ++++--- modules/local/gatk4/mergevcfs/functions.nf | 68 ----------- .../local/gatk4/splitncigarreads/functions.nf | 68 ----------- modules/local/gatk4/splitncigarreads/meta.yml | 40 ------- .../gatk4/variantfiltration/functions.nf | 68 ----------- .../gatk4/indexfeaturefile/functions.nf | 78 +++++++++++++ .../modules/gatk4/indexfeaturefile}/main.nf | 31 +++-- .../modules/gatk4/indexfeaturefile/meta.yml | 42 +++++++ .../modules/gatk4/mergevcfs/functions.nf | 78 +++++++++++++ .../modules}/gatk4/mergevcfs/main.nf | 18 +-- .../modules}/gatk4/mergevcfs/meta.yml | 7 +- .../gatk4/variantfiltration/functions.nf | 78 +++++++++++++ .../modules}/gatk4/variantfiltration/main.nf | 24 ++-- .../modules}/gatk4/variantfiltration/meta.yml | 7 +- subworkflows/local/input_check.nf | 1 + workflows/rnavar.nf | 76 ++++++++++++- 26 files changed, 545 insertions(+), 538 deletions(-) create mode 100644 assets/samplesheet_full.csv delete mode 100644 modules/local/gatk4/applybqsr/functions.nf delete mode 100644 modules/local/gatk4/applybqsr/main.nf delete mode 100644 modules/local/gatk4/applybqsr/meta.yml delete mode 100644 modules/local/gatk4/mergevcfs/functions.nf delete mode 100644 modules/local/gatk4/splitncigarreads/functions.nf delete mode 100644 modules/local/gatk4/splitncigarreads/meta.yml delete mode 100644 modules/local/gatk4/variantfiltration/functions.nf create mode 100644 modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf rename modules/{local/gatk4/splitncigarreads => nf-core/modules/gatk4/indexfeaturefile}/main.nf (52%) create mode 100644 modules/nf-core/modules/gatk4/indexfeaturefile/meta.yml create mode 100644 modules/nf-core/modules/gatk4/mergevcfs/functions.nf rename modules/{local => nf-core/modules}/gatk4/mergevcfs/main.nf (66%) rename modules/{local => nf-core/modules}/gatk4/mergevcfs/meta.yml (90%) create mode 100644 modules/nf-core/modules/gatk4/variantfiltration/functions.nf rename modules/{local => nf-core/modules}/gatk4/variantfiltration/main.nf (56%) rename modules/{local => nf-core/modules}/gatk4/variantfiltration/meta.yml (91%) diff --git a/assets/samplesheet_full.csv b/assets/samplesheet_full.csv new file mode 100644 index 00000000..36211ac7 --- /dev/null +++ b/assets/samplesheet_full.csv @@ -0,0 +1,5 @@ +sample,fastq_1,fastq_2,strandedness +GM12878,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603629_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603629_T1_2.fastq.gz,reverse +GM12878,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603630_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603630_T1_2.fastq.gz,reverse +K562,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603392_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603392_T1_2.fastq.gz,reverse +K562,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603393_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603393_T1_2.fastq.gz,reverse diff --git a/assets/samplesheet_test.csv b/assets/samplesheet_test.csv index 7131bd5d..fc6dfa21 100644 --- a/assets/samplesheet_test.csv +++ b/assets/samplesheet_test.csv @@ -1,2 +1,4 @@ sample,fastq_1,fastq_2,strandedness GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +TEST1,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 7634b236..91327e5c 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -1,7 +1,4 @@ -#!/usr/bin/env python - -# TODO nf-core: Update the script to check the samplesheet -# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv +#!/usr/bin/env python3 import os import sys @@ -10,7 +7,7 @@ def parse_args(args=None): - Description = "Reformat nf-core/rnavar samplesheet file and check its contents." + Description = "Reformat nf-core/rnaseq samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) @@ -29,63 +26,66 @@ def make_dir(path): def print_error(error, context="Line", context_str=""): - error_str = "ERROR: Please check samplesheet -> {}".format(error) + error_str = f"ERROR: Please check samplesheet -> {error}" if context != "" and context_str != "": - error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( - error, context.strip(), context_str.strip() - ) + error_str = f"ERROR: Please check samplesheet -> {error}\n{context.strip()}: '{context_str.strip()}'" print(error_str) sys.exit(1) -# TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + sample,fastq_1,fastq_2,strandedness + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,forward + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,forward + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,,forward For an example see: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + https://github.com/nf-core/test-datasets/blob/rnaseq/samplesheet/v3.1/samplesheet_test.csv """ sample_mapping_dict = {} - with open(file_in, "r") as fin: + with open(file_in, "r", encoding='utf-8-sig') as fin: ## Check header - MIN_COLS = 2 - # TODO nf-core: Update the column names for the input samplesheet - HEADER = ["sample", "fastq_1", "fastq_2"] + MIN_COLS = 3 + HEADER = ["sample", "fastq_1", "fastq_2", "strandedness"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) + print( + f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}" + ) sys.exit(1) ## Check sample entries for line in fin: lspl = [x.strip().strip('"') for x in line.strip().split(",")] - # Check valid number of columns per row + ## Check valid number of columns per row if len(lspl) < len(HEADER): print_error( - "Invalid number of columns (minimum = {})!".format(len(HEADER)), + f"Invalid number of columns (minimum = {len(HEADER)})!", "Line", line, ) + num_cols = len([x for x in lspl if x]) if num_cols < MIN_COLS: print_error( - "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), + f"Invalid number of populated columns (minimum = {MIN_COLS})!", "Line", line, ) ## Check sample name entries - sample, fastq_1, fastq_2 = lspl[: len(HEADER)] - sample = sample.replace(" ", "_") + sample, fastq_1, fastq_2, strandedness = lspl[: len(HEADER)] + if sample.find(" ") != -1: + print( + f"WARNING: Spaces have been replaced by underscores for sample: {sample}" + ) + sample = sample.replace(" ", "_") if not sample: print_error("Sample entry has not been specified!", "Line", line) @@ -101,16 +101,32 @@ def check_samplesheet(file_in, file_out): line, ) + ## Check strandedness + strandednesses = ["unstranded", "forward", "reverse"] + if strandedness: + if strandedness not in strandednesses: + print_error( + f"Strandedness must be one of '{', '.join(strandednesses)}'!", + "Line", + line, + ) + else: + print_error( + f"Strandedness has not been specified! Must be one of {', '.join(strandednesses)}.", + "Line", + line, + ) + ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2] + sample_info = [] ## [single_end, fastq_1, fastq_2, strandedness] if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2] + sample_info = ["0", fastq_1, fastq_2, strandedness] elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2] + sample_info = ["1", fastq_1, fastq_2, strandedness] else: print_error("Invalid combination of columns provided!", "Line", line) - ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] } + ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, strandedness ]]} if sample not in sample_mapping_dict: sample_mapping_dict[sample] = [sample_info] else: @@ -124,17 +140,38 @@ def check_samplesheet(file_in, file_out): out_dir = os.path.dirname(file_out) make_dir(out_dir) with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") + fout.write( + ",".join(["sample", "single_end", "fastq_1", "fastq_2", "strandedness"]) + + "\n" + ) for sample in sorted(sample_mapping_dict.keys()): - ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) + ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + if not all( + x[0] == sample_mapping_dict[sample][0][0] + for x in sample_mapping_dict[sample] + ): + print_error( + f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!", + "Sample", + sample, + ) + + ## Check that multiple runs of the same sample are of the same strandedness + if not all( + x[-1] == sample_mapping_dict[sample][0][-1] + for x in sample_mapping_dict[sample] + ): + print_error( + f"Multiple runs of a sample must have the same strandedness!", + "Sample", + sample, + ) for idx, val in enumerate(sample_mapping_dict[sample]): - fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") + fout.write(",".join([f"{sample}_T{idx+1}"] + val) + "\n") else: - print_error("No entries to process!", "Samplesheet: {}".format(file_in)) + print_error(f"No entries to process!", "Samplesheet: {file_in}") def main(args=None): diff --git a/conf/modules.config b/conf/modules.config index 865c1b44..3c32bd82 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -112,12 +112,19 @@ params { publish_dir = 'variant_calling' publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] } + 'gatk_indexfeaturefile' { + args = '' + suffix = '.haplotypecaller' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] + } 'gatk_variantfilter' { args = '' suffix = '.filtered' publish_by_meta = true publish_dir = 'variant_filtering' - publish_files = ['vcf':'', 'vcf.idx':''] + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] } 'fastqc' { args = "--quiet" diff --git a/conf/test.config b/conf/test.config index c56b48c1..8e85b5ac 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,8 @@ params { max_time = 6.h // Input data - input = 'assets/samplesheet_test.csv' + //input = 'assets/samplesheet_test.csv' + input = 'assets/samplesheet_full.csv' // Genome references fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' diff --git a/modules.json b/modules.json index 5b2e7d52..c2cfbdf4 100644 --- a/modules.json +++ b/modules.json @@ -24,12 +24,21 @@ "gatk4/createsequencedictionary": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, + "gatk4/indexfeaturefile": { + "git_sha": "1a4c7cec1b9d82fdaa15897d8e9a9e9a4767444d" + }, "gatk4/intervallisttools": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, + "gatk4/mergevcfs": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, "gatk4/splitncigarreads": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, + "gatk4/variantfiltration": { + "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + }, "gffread": { "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" }, diff --git a/modules/local/gatk4/applybqsr/functions.nf b/modules/local/gatk4/applybqsr/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/applybqsr/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/applybqsr/main.nf b/modules/local/gatk4/applybqsr/main.nf deleted file mode 100644 index 8e10a5f2..00000000 --- a/modules/local/gatk4/applybqsr/main.nf +++ /dev/null @@ -1,46 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_APPLYBQSR { - tag "$meta.id" - label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } - - input: - tuple val(meta), path(bam), path(bai), path(bqsr_table), path(intervals) - path fasta - path fastaidx - path dict - - output: - tuple val(meta), path("*.bam"), emit: bam - path "*.version.txt" , emit: version - - script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def interval = intervals ? "-L ${intervals}" : "" - """ - gatk ApplyBQSR \\ - -R $fasta \\ - -I $bam \\ - --bqsr-recal-file $bqsr_table \\ - $interval \\ - -O ${prefix}.bam \\ - $options.args - - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt - """ -} diff --git a/modules/local/gatk4/applybqsr/meta.yml b/modules/local/gatk4/applybqsr/meta.yml deleted file mode 100644 index 9bf12f09..00000000 --- a/modules/local/gatk4/applybqsr/meta.yml +++ /dev/null @@ -1,58 +0,0 @@ -name: gatk4_applybqsr -description: Apply base quality score recalibration (BQSR) to a bam file -keywords: - - bqsr - - bam -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file from alignment - pattern: "*.{bam}" - - bqsr_table: - type: file - description: Recalibration table from gatk4_baserecalibrator - - fasta: - type: file - description: The reference fasta file - - fastaidx: - type: file - description: Index of reference fasta file - - dict: - type: file - description: GATK sequence dictionary - - intervalsBed: - type: file - description: Bed file with the genomic regions included in the library (optional) - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - version: - type: file - description: File containing software version - pattern: "*.{version.txt}" - - bam: - type: file - description: Recalibrated BAM file - pattern: "*.{bam}" - -authors: - - "@yocra3" diff --git a/modules/local/gatk4/haplotypecaller/functions.nf b/modules/local/gatk4/haplotypecaller/functions.nf index da9da093..85628ee0 100644 --- a/modules/local/gatk4/haplotypecaller/functions.nf +++ b/modules/local/gatk4/haplotypecaller/functions.nf @@ -9,6 +9,13 @@ def getSoftwareName(task_process) { return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() } +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + // // Function to initialise default values and to generate a Groovy Map of available options for nf-core modules // @@ -37,32 +44,35 @@ def getPathFromList(path_list) { // Function to save/publish module results // def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] } + path = path instanceof String ? path : '' + path_list.add(path) } } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" } } diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf index da90ba4b..fc0ee0e9 100644 --- a/modules/local/gatk4/haplotypecaller/main.nf +++ b/modules/local/gatk4/haplotypecaller/main.nf @@ -1,21 +1,21 @@ // Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process GATK4_HAPLOTYPECALLER { tag "$meta.id" - label 'process_low' + label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" } input: @@ -28,33 +28,35 @@ process GATK4_HAPLOTYPECALLER { val no_intervals output: - tuple val(meta), path("*.vcf") , emit: vcf - tuple val(meta), path(interval), path("*.vcf"), emit: interval_vcf - path "*.version.txt" , emit: version + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path(interval), path("*.vcf.gz") , emit: interval_vcf + path "versions.yml" , emit: versions script: - def software = getSoftwareName(task.process) def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" - def avail_mem = 3 + def avail_mem = 3 if (!task.memory) { log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } - def intervalsOptions = no_intervals ? "" : "-L ${interval}" - def dbsnpOptions = dbsnp ? "-D ${dbsnp}" : "" - //TODO allow ploidy argument here since we allow it for the cnv callers? or is this covered with options? Might unintuitive to use + def interval_option = no_intervals ? "" : "-L ${interval}" + def dbsnp_option = dbsnp ? "-D ${dbsnp}" : "" """ gatk \\ --java-options "-Xmx${avail_mem}g" \\ HaplotypeCaller \\ -R $fasta \\ -I $bam \\ - ${dbsnpOptions} \\ - ${intervalsOptions} \\ - -O ${prefix}.vcf \\ - $options.args + ${dbsnp_option} \\ + ${interval_option} \\ + -O ${prefix}.vcf.gz \\ + $options.args \\ + --tmp-dir . - gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS """ } diff --git a/modules/local/gatk4/mergevcfs/functions.nf b/modules/local/gatk4/mergevcfs/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/mergevcfs/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/splitncigarreads/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/local/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml deleted file mode 100644 index e433cbf6..00000000 --- a/modules/local/gatk4/splitncigarreads/meta.yml +++ /dev/null @@ -1,40 +0,0 @@ -name: gatk4_splitncigarreads -description: Splits reads that contain Ns in their cigar string -keywords: - - vcf - - merge -tools: - - gatk4: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test'] - - bam: - type: list - description: BAM/SAM/CRAM file containing reads - pattern: "*.{bam,sam,cram}" - - fasta: - type: tuple of files - description: | - Tuple of fasta file (first), sequence dict (second) and fasta index (third) - pattern: ["*.fasta", "*.dict", "*.fai"] -output: - - bam: - type: file - description: Output file with split reads (BAM/SAM/CRAM) - pattern: "*.{bam,sam,cram}" - - version: - type: file - description: File containing software version - pattern: "*.version.txt" -authors: - - "@kevinmenden" diff --git a/modules/local/gatk4/variantfiltration/functions.nf b/modules/local/gatk4/variantfiltration/functions.nf deleted file mode 100644 index da9da093..00000000 --- a/modules/local/gatk4/variantfiltration/functions.nf +++ /dev/null @@ -1,68 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/local/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf similarity index 52% rename from modules/local/gatk4/splitncigarreads/main.nf rename to modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index d1c328ea..8f40a3e3 100644 --- a/modules/local/gatk4/splitncigarreads/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -1,12 +1,12 @@ // Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) -process GATK4_SPLITNCIGARREADS { +process GATK4_INDEXFEATUREFILE { tag "$meta.id" - label 'process_medium' + label 'process_low' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } @@ -19,25 +19,22 @@ process GATK4_SPLITNCIGARREADS { } input: - tuple val(meta), path(bam) - path(fasta) - path(fai) - path(dict) + tuple val(meta), path(feature_file) output: - tuple val(meta), path('*.bam'), path('*.bai'), emit: bam - path '*.version.txt' , emit: version + tuple val(meta), path("*.{tbi,idx}"), emit: index + path "versions.yml" , emit: versions script: - def software = getSoftwareName(task.process) - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ - gatk SplitNCigarReads \\ - -R $fasta \\ - -I $bam \\ - -O ${prefix}.bam \\ - $options.args + gatk \\ + IndexFeatureFile \\ + $options.args \\ + -I $feature_file - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/meta.yml b/modules/nf-core/modules/gatk4/indexfeaturefile/meta.yml new file mode 100644 index 00000000..eebe6b85 --- /dev/null +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/meta.yml @@ -0,0 +1,42 @@ +name: gatk4_indexfeaturefile +description: Creates an index for a feature file, e.g. VCF or BED file. +keywords: + - index + - feature +tools: + - gatk4: + description: Genome Analysis Toolkit (GATK4) + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + tool_dev_url: https://github.com/broadinstitute/gatk + doi: "10.1158/1538-7445.AM2017-3590" + licence: ['BSD-3-clause'] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - feature_file: + type: file + description: VCF/BED file + pattern: "*.{vcf,vcf.gz,bed,bed.gz}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: Index for VCF/BED file + pattern: "*.{tbi,idx}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@santiagorevale" diff --git a/modules/nf-core/modules/gatk4/mergevcfs/functions.nf b/modules/nf-core/modules/gatk4/mergevcfs/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/mergevcfs/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/local/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf similarity index 66% rename from modules/local/gatk4/mergevcfs/main.nf rename to modules/nf-core/modules/gatk4/mergevcfs/main.nf index 8b9747bc..28073fcb 100644 --- a/modules/local/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -1,5 +1,5 @@ // Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) @@ -11,11 +11,11 @@ process GATK4_MERGEVCFS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" } input: @@ -24,11 +24,10 @@ process GATK4_MERGEVCFS { val use_ref_dict output: - tuple val(meta), path('*.vcf.gz'), path('*.vcf.gz.tbi'), emit: vcf - path '*.version.txt' , emit: version + tuple val(meta), path('*.vcf.gz'), emit: vcf + path "versions.yml" , emit: versions script: - def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" // Make list of VCFs to merge @@ -44,6 +43,9 @@ process GATK4_MERGEVCFS { $ref \\ $options.args - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS """ } diff --git a/modules/local/gatk4/mergevcfs/meta.yml b/modules/nf-core/modules/gatk4/mergevcfs/meta.yml similarity index 90% rename from modules/local/gatk4/mergevcfs/meta.yml rename to modules/nf-core/modules/gatk4/mergevcfs/meta.yml index 14b28fa0..597f9ec6 100644 --- a/modules/local/gatk4/mergevcfs/meta.yml +++ b/modules/nf-core/modules/gatk4/mergevcfs/meta.yml @@ -12,6 +12,7 @@ tools: homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] input: - meta: type: map @@ -34,9 +35,9 @@ output: type: file description: merged vcf file pattern: "*.vcf.gz" - - version: + - versions: type: file - description: File containing software version - pattern: "*.version.txt" + description: File containing software versions + pattern: "versions.yml" authors: - "@kevinmenden" diff --git a/modules/nf-core/modules/gatk4/variantfiltration/functions.nf b/modules/nf-core/modules/gatk4/variantfiltration/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/gatk4/variantfiltration/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/local/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf similarity index 56% rename from modules/local/gatk4/variantfiltration/main.nf rename to modules/nf-core/modules/gatk4/variantfiltration/main.nf index d442aba8..c2c7a0b1 100644 --- a/modules/local/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -1,5 +1,5 @@ // Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) @@ -11,34 +11,36 @@ process GATK4_VARIANTFILTRATION { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" + container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" + container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" } input: - tuple val(meta), path(vcf), path (tbi) + tuple val(meta), path(vcf), path(vcf_tbi) path fasta path fai path dict output: - tuple val(meta), path("*.vcf"), emit: vcf - path "*.version.txt" , emit: version - + tuple val(meta), path("*.vcf.gz"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi + path "versions.yml" , emit: versions script: - def software = getSoftwareName(task.process) def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" """ gatk VariantFiltration \\ -R $fasta \\ -V $vcf \\ - -O ${prefix}.vcf \\ + -O ${prefix}.vcf.gz \\ $options.args - echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//' > ${software}.version.txt + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS """ } diff --git a/modules/local/gatk4/variantfiltration/meta.yml b/modules/nf-core/modules/gatk4/variantfiltration/meta.yml similarity index 91% rename from modules/local/gatk4/variantfiltration/meta.yml rename to modules/nf-core/modules/gatk4/variantfiltration/meta.yml index d7f72582..6d4983a6 100644 --- a/modules/local/gatk4/variantfiltration/meta.yml +++ b/modules/nf-core/modules/gatk4/variantfiltration/meta.yml @@ -12,6 +12,7 @@ tools: homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 + licence: ['Apache-2.0'] input: - meta: type: map @@ -39,9 +40,9 @@ output: type: file description: filtered VCF file pattern: "*.filtered.{vcf}" - - version: + - versions: type: file - description: File containing software version - pattern: "*.version.txt" + description: File containing software versions + pattern: "versions.yml" authors: - "@kevinmenden" diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index c1b07196..7edb4f75 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -26,6 +26,7 @@ workflow INPUT_CHECK { def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample + meta.sample = row.sample meta.single_end = row.single_end.toBoolean() def array = [] diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index c930aaa4..1a9cba60 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -79,6 +79,7 @@ prepareToolIndices = params.aligner def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] +def untar_options = [publish_files: false] if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false @@ -109,14 +110,16 @@ include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baser include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) -include { GATK4_MERGEVCFS } from '../modules/local/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) -include { GATK4_VARIANTFILTRATION } from '../modules/local/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) +include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' addParams(options: modules['gatk_indexfeaturefile']) +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, - star_index_options: modules['star_genomegenerate'] + star_index_options: modules['star_genomegenerate'], + star_untar_options: untar_options ) include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: modules['star_align'], @@ -288,6 +291,73 @@ workflow RNAVAR { bam_recalibrated_qc = RECALIBRATE.out.qc ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) + // MODULE: IntervalListTools from GATK4 + ch_interval_list_split = Channel.empty() + + if (!params.skip_intervallisttools) { + GATK4_INTERVALLISTTOOLS(ch_interval_list) + ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() + } + else ch_interval_list_split = ch_interval_list + + // MODULE: HaplotypeCaller from GATK4 + interval_flag = params.no_intervals + haplotypecaller_vcf = Channel.empty() + + haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) + .map{ meta, bam, bai, interval_list -> + new_meta = meta.clone() + new_meta.id = meta.id + "_" + interval_list.baseName + [new_meta, bam, bai, interval_list]} + + GATK4_HAPLOTYPECALLER( + haplotypecaller_interval_bam, + params.dbsnp_vcf, + params.dbsnp_vcf_index, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + interval_flag + ) + + haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf + .map{ meta, vcf -> + meta.id = meta.sample + [meta, vcf]} + .groupTuple() + + ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLER.out.versions.first().ifEmpty(null)) + use_ref_dict = true + + GATK4_MERGEVCFS( + haplotypecaller_raw, + PREPARE_GENOME.out.dict, + use_ref_dict + ) + haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf + ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) + + GATK4_INDEXFEATUREFILE( + haplotypecaller_vcf + ) + haplotypecaller_vcf_tbi = haplotypecaller_vcf.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) + ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) + + // MODULE: VariantFiltration from GATK4 + if (!params.skip_variantfiltration) { + + GATK4_VARIANTFILTRATION( + haplotypecaller_vcf_tbi, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict + ) + + filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf + filtered_vcf_tbi = GATK4_VARIANTFILTRATION.out.tbi + ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) + } + } CUSTOM_DUMPSOFTWAREVERSIONS ( From 5fbb84d2f881295f7fa3cc4299439a891a0468f4 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 18 Nov 2021 16:02:08 +0100 Subject: [PATCH 048/235] Fix: issue with cat_fastq; tested with multiple replicates --- assets/samplesheet_full.csv | 7 +++---- assets/samplesheet_test.csv | 2 -- conf/test.config | 3 +-- subworkflows/local/input_check.nf | 1 - workflows/rnavar.nf | 5 +++-- 5 files changed, 7 insertions(+), 11 deletions(-) diff --git a/assets/samplesheet_full.csv b/assets/samplesheet_full.csv index 36211ac7..6b6e0ec5 100644 --- a/assets/samplesheet_full.csv +++ b/assets/samplesheet_full.csv @@ -1,5 +1,4 @@ sample,fastq_1,fastq_2,strandedness -GM12878,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603629_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603629_T1_2.fastq.gz,reverse -GM12878,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603630_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603630_T1_2.fastq.gz,reverse -K562,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603392_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603392_T1_2.fastq.gz,reverse -K562,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603393_T1_1.fastq.gz,s3://nf-core-awsmegatests/rnaseq/input_data/SRX1603393_T1_2.fastq.gz,reverse +RAP1_UNINDUCED_REP1,s3://nf-core-awsmegatests/rnaseq/input_data/minimal/GSE110004/SRR6357073_1.fastq.gz,,reverse +RAP1_UNINDUCED_REP2,s3://nf-core-awsmegatests/rnaseq/input_data/minimal/GSE110004/SRR6357074_1.fastq.gz,,reverse +RAP1_UNINDUCED_REP2,s3://nf-core-awsmegatests/rnaseq/input_data/minimal/GSE110004/SRR6357075_1.fastq.gz,,reverse diff --git a/assets/samplesheet_test.csv b/assets/samplesheet_test.csv index fc6dfa21..7131bd5d 100644 --- a/assets/samplesheet_test.csv +++ b/assets/samplesheet_test.csv @@ -1,4 +1,2 @@ sample,fastq_1,fastq_2,strandedness GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -TEST1,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -TEST2,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/conf/test.config b/conf/test.config index 8e85b5ac..c56b48c1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,8 +20,7 @@ params { max_time = 6.h // Input data - //input = 'assets/samplesheet_test.csv' - input = 'assets/samplesheet_full.csv' + input = 'assets/samplesheet_test.csv' // Genome references fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 7edb4f75..c1b07196 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -26,7 +26,6 @@ workflow INPUT_CHECK { def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample - meta.sample = row.sample meta.single_end = row.single_end.toBoolean() def array = [] diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 1a9cba60..080f0bf3 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -150,6 +150,7 @@ def multiqc_report = [] workflow RNAVAR { ch_versions = Channel.empty() + ch_fastq = Channel.empty() // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -307,7 +308,7 @@ workflow RNAVAR { haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() - new_meta.id = meta.id + "_" + interval_list.baseName + new_meta.id = meta.id [new_meta, bam, bai, interval_list]} GATK4_HAPLOTYPECALLER( @@ -322,7 +323,7 @@ workflow RNAVAR { haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf .map{ meta, vcf -> - meta.id = meta.sample + meta.id = meta.id [meta, vcf]} .groupTuple() From ebbc46958f381f80628e5e4fbc5f735bf3a64993 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 18 Nov 2021 16:14:38 +0100 Subject: [PATCH 049/235] fix: lint warnings --- modules.json | 4 ++-- .../modules/gatk4/baserecalibrator/main.nf | 2 +- .../modules/gatk4/baserecalibrator/meta.yml | 5 ++++- .../modules/gatk4/splitncigarreads/main.nf | 6 +++--- .../modules/gatk4/splitncigarreads/meta.yml | 15 +++++++++++---- 5 files changed, 21 insertions(+), 11 deletions(-) diff --git a/modules.json b/modules.json index c2cfbdf4..f4e7f171 100644 --- a/modules.json +++ b/modules.json @@ -16,7 +16,7 @@ "git_sha": "34268347447cd60013b69279a42aa9d081592735" }, "gatk4/baserecalibrator": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "34268347447cd60013b69279a42aa9d081592735" }, "gatk4/bedtointervallist": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" @@ -34,7 +34,7 @@ "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, "gatk4/splitncigarreads": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "691feeafdcd8b7874f540695ece122cce01a524f" }, "gatk4/variantfiltration": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index ff9eb1f9..ce6f5906 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -21,7 +21,7 @@ process GATK4_BASERECALIBRATOR { input: tuple val(meta), path(input), path(input_index) path fasta - path fastaidx + path fai path dict path intervalsBed path knownSites diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml index 7fd273e1..188340b4 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml +++ b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml @@ -31,12 +31,15 @@ input: - fasta: type: file description: The reference fasta file - - fastaidx: + pattern: "*.fasta" + - fai: type: file description: Index of reference fasta file + pattern: "*.fasta.fai" - dict: type: file description: GATK sequence dictionary + pattern: "*.dict" - intervalsBed: type: file description: Bed file with the genomic regions included in the library (optional) diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf index a044b1a9..26fb799d 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf +++ b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf @@ -20,9 +20,9 @@ process GATK4_SPLITNCIGARREADS { input: tuple val(meta), path(bam) - path(fasta) - path(fai) - path(dict) + path fasta + path fai + path dict output: tuple val(meta), path('*.bam'), emit: bam diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml index f287ede4..fd6edda0 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml +++ b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml @@ -24,10 +24,17 @@ input: description: BAM/SAM/CRAM file containing reads pattern: "*.{bam,sam,cram}" - fasta: - type: tuple of files - description: | - Tuple of fasta file (first), sequence dict (second) and fasta index (third) - pattern: ["*.fasta", "*.dict", "*.fai"] + type: file + description: The reference fasta file + pattern: "*.fasta" + - fai: + type: file + description: Index of reference fasta file + pattern: "*.fasta.fai" + - dict: + type: file + description: GATK sequence dictionary + pattern: "*.dict" output: - bam: type: file From 4bc97fb54f5261be00dc1c8b00aca0958da31a37 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 18 Nov 2021 16:35:17 +0100 Subject: [PATCH 050/235] fix: lint editorconfig error --- workflows/rnavar.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 080f0bf3..c6b7c3b8 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -234,7 +234,7 @@ workflow RNAVAR { if (params.bam_csi_index) ch_genome_bam_index = ALIGN_STAR.out.csi ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) - // SUBWORKFLOW: Mark duplicates with Picard + // SUBWORKFLOW: Mark duplicates with Picard MARKDUPLICATES(ch_genome_bam) ch_genome_bam = MARKDUPLICATES.out.bam ch_genome_bam_index = MARKDUPLICATES.out.bai From ffab2a1923a4b7b2873a4efb82acf88fc03c0675 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 19 Nov 2021 10:29:46 +0100 Subject: [PATCH 051/235] update: samtools and picard modules updated to latest version from nf-core. --- modules.json | 18 +++++++++--------- modules/local/get_chrom_sizes.nf | 4 ++-- modules/local/gtf2bed.nf | 2 +- modules/local/samplesheet_check.nf | 2 +- .../modules/gatk4/variantfiltration/main.nf | 12 +++++++++--- .../modules/gatk4/variantfiltration/meta.yml | 16 ++++++++++++---- .../modules/picard/markduplicates/main.nf | 6 +++--- modules/nf-core/modules/samtools/faidx/main.nf | 6 +++--- .../nf-core/modules/samtools/flagstat/main.nf | 8 ++++---- .../nf-core/modules/samtools/idxstats/main.nf | 6 +++--- modules/nf-core/modules/samtools/index/main.nf | 9 +++++---- modules/nf-core/modules/samtools/merge/main.nf | 9 +++++---- modules/nf-core/modules/samtools/sort/main.nf | 6 +++--- modules/nf-core/modules/samtools/stats/main.nf | 9 +++++---- nextflow.config | 3 +++ 15 files changed, 68 insertions(+), 48 deletions(-) diff --git a/modules.json b/modules.json index f4e7f171..b4bb9975 100644 --- a/modules.json +++ b/modules.json @@ -37,7 +37,7 @@ "git_sha": "691feeafdcd8b7874f540695ece122cce01a524f" }, "gatk4/variantfiltration": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "43a1c1c6d18d7d9395846503046f1649ba72a06e" }, "gffread": { "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" @@ -49,28 +49,28 @@ "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, "picard/markduplicates": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "4e5b6ed843ee0691848aba58088e4347cd3aae98" }, "samtools/faidx": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/flagstat": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/idxstats": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/index": { - "git_sha": "1abe23e1592f7cf6a3724589061827df0d8a0252" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/merge": { - "git_sha": "ac1e6df076195cec553a2079c9cebd94026a0d47" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/sort": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "samtools/stats": { - "git_sha": "ac1e6df076195cec553a2079c9cebd94026a0d47" + "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, "star/align": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf index 95957db1..1f582d8c 100644 --- a/modules/local/get_chrom_sizes.nf +++ b/modules/local/get_chrom_sizes.nf @@ -21,8 +21,8 @@ process GET_CHROM_SIZES { path fasta output: - path '*.sizes' , emit: sizes - path '*.fai' , emit: fai + path "*.sizes" , emit: sizes + path "*.fai" , emit: fai path "versions.yml", emit: versions script: diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index ea2519c8..d6ceaff3 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -21,7 +21,7 @@ process GTF2BED { path gtf output: - path '*.bed' , emit: bed + path "*.bed" , emit: bed path "versions.yml", emit: versions script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index dc0dfc7b..2c98eb99 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -21,7 +21,7 @@ process SAMPLESHEET_CHECK { path samplesheet output: - path '*.csv' , emit: csv + path "*.csv" , emit: csv path "versions.yml", emit: versions script: // This script is bundled with the pipeline, in nf-core/rnavar/bin/ diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index c2c7a0b1..e0f0727a 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -27,12 +27,18 @@ process GATK4_VARIANTFILTRATION { output: tuple val(meta), path("*.vcf.gz"), emit: vcf tuple val(meta), path("*.tbi") , emit: tbi - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.toGiga() + } """ - gatk VariantFiltration \\ + gatk --java-options "-Xmx${avail_mem}G" VariantFiltration \\ -R $fasta \\ -V $vcf \\ -O ${prefix}.vcf.gz \\ diff --git a/modules/nf-core/modules/gatk4/variantfiltration/meta.yml b/modules/nf-core/modules/gatk4/variantfiltration/meta.yml index 6d4983a6..71f0b8b2 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/meta.yml +++ b/modules/nf-core/modules/gatk4/variantfiltration/meta.yml @@ -21,8 +21,12 @@ input: e.g. [ id:'test'] - vcf: type: list - description: Input VCF file - pattern: "*.{vcf}" + description: List of VCF(.gz) files + pattern: "*.{vcf,vcf.gz}" + - vcf_tbi: + type: list + description: List of VCF file indexes + pattern: "*.{idx,tbi}" - fasta: type: file description: Fasta file of reference genome @@ -38,8 +42,12 @@ input: output: - vcf: type: file - description: filtered VCF file - pattern: "*.filtered.{vcf}" + description: Compressed VCF file + pattern: "*.vcf.gz" + - tbi: + type: file + description: Index of VCF file + pattern: "*.vcf.gz.tbi" - versions: type: file description: File containing software versions diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index b34e3113..130a1e52 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -40,9 +40,9 @@ process PICARD_MARKDUPLICATES { -Xmx${avail_mem}g \\ MarkDuplicates \\ $options.args \\ - INPUT=$bam \\ - OUTPUT=${prefix}.bam \\ - METRICS_FILE=${prefix}.MarkDuplicates.metrics.txt + I=$bam \\ + O=${prefix}.bam \\ + M=${prefix}.MarkDuplicates.metrics.txt cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index 80cedeab..80708084 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_FAIDX { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index f9115c6b..072a135f 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_FLAGSTAT { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: @@ -27,7 +27,7 @@ process SAMTOOLS_FLAGSTAT { script: """ - samtools flagstat $bam > ${bam}.flagstat + samtools flagstat --threads ${task.cpus-1} $bam > ${bam}.flagstat cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index b005088a..fa0e7dc3 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_IDXSTATS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index 62254bc8..d66e4513 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_INDEX { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: @@ -29,7 +29,8 @@ process SAMTOOLS_INDEX { script: """ - samtools index $options.args $input + samtools index -@ ${task.cpus-1} $options.args $input + cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index fefb423b..ab641bb9 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_MERGE { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: @@ -32,7 +32,8 @@ process SAMTOOLS_MERGE { def file_type = input_files[0].getExtension() def reference = fasta ? "--reference ${fasta}" : "" """ - samtools merge ${reference} ${prefix}.${file_type} $input_files + samtools merge --threads ${task.cpus-1} $options.args ${reference} ${prefix}.${file_type} $input_files + cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index b30f6f45..f980b472 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_SORT { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index aab43410..e0a2b50d 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -11,11 +11,11 @@ process SAMTOOLS_STATS { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::samtools=1.13' : null) + conda (params.enable_conda ? "bioconda::samtools=1.14" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0" + container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" } else { - container "quay.io/biocontainers/samtools:1.13--h8c37831_0" + container "quay.io/biocontainers/samtools:1.14--hb421002_0" } input: @@ -29,7 +29,8 @@ process SAMTOOLS_STATS { script: def reference = fasta ? "--reference ${fasta}" : "" """ - samtools stats ${reference} ${input} > ${input}.stats + samtools stats --threads ${task.cpus-1} ${reference} ${input} > ${input}.stats + cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/nextflow.config b/nextflow.config index 3d8c5c44..cdf4a8f2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,6 +13,9 @@ params { // Mandatory option input = null // sample sheet + fasta = null + fasta_dict = null + fasta_fai = null // Genome and reference options genome = null From 9ea279e5de16cfab5dba91a46cd4155f1bddfe6f Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 19 Nov 2021 11:58:50 +0100 Subject: [PATCH 052/235] fix: lint warnings --- README.md | 40 ++++++++++++----------- conf/base.config | 2 -- conf/test_full.config | 11 +++---- docs/output.md | 29 ++++++++++++++--- docs/usage.md | 70 ++++++++++++++++++++++++++++++++--------- lib/WorkflowMain.groovy | 1 - 6 files changed, 105 insertions(+), 48 deletions(-) diff --git a/README.md b/README.md index 8793c99c..e2b5e231 100644 --- a/README.md +++ b/README.md @@ -16,20 +16,26 @@ ## Introduction - -**nf-core/rnavar** is a bioinformatics best-practice analysis pipeline for GATK4 RNA variant calling pipeline. +**nf-core/rnavar** bioinformatics best-practice pipeline for RNA-Seq variant analysis. The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! - -On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnavar/results). - ## Pipeline summary - - -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +1. Merge re-sequenced FastQ files ([`cat`](http://www.linfo.org/cat.html)) +2. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +3. Align reads to reference genome ([`STAR`](https://github.com/alexdobin/STAR)) +4. Sort and index alignments ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) +5. Duplicate read marking ([`Picard MarkDuplicates`](https://broadinstitute.github.io/picard/)) +6. Splits reads that contain Ns in their cigar string ([`GATK4 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) +7. Estimate and correct systematic bias using base quality score recalibration ([`GATK4 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK4 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) +8. Convert a BED file to a Picard Interval List ([`Picard BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) +9. Scatter one interval-list into many interval-files ([`Picard IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) +10. Call SNPs and indels ([`GATK4 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) +11. Merge multiple VCF files into one VCF ([`GATK4 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) +12. Index the VCF ([`GATK4 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) +13. Filter variant calls based on certain criteria ([`GATK4 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) +14. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC`](http://multiqc.info/), [`R`](https://www.r-project.org/)) ## Quick Start @@ -49,10 +55,8 @@ On release, automated continuous integration tests run the pipeline on a full-si 4. Start running your own analysis! - - ```console - nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh37 + nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh38 ``` ## Documentation @@ -61,11 +65,13 @@ The nf-core/rnavar pipeline comes with documentation about the pipeline [usage]( ## Credits -nf-core/rnavar was originally written by @praveenraj2018. +These scripts were originally written in Nextflow DSL2 for use at the [Barntumörbanken, Karolinska Institutet](https://ki.se/forskning/barntumorbanken) by Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018)) and Maxime U. Garcia ([@maxulysse](https://github.com/maxulysse)). -We thank the following people for their extensive assistance in the development of this pipeline: +The pipeline is primarily maintained by Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018)) and Maxime U. Garcia ([@maxulysse](https://github.com/maxulysse)) from [Barntumörbanken, Karolinska Institutet](https://ki.se/forskning/barntumorbanken). - +Many thanks to other who have helped out along the way too, including (but not limited to): +[@ewels](https://github.com/ewels), +[@drpatelh](https://github.com/drpatelh). ## Contributions and Support @@ -75,10 +81,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations - - - - An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. You can cite the `nf-core` publication as follows: diff --git a/conf/base.config b/conf/base.config index 1e805048..a60d9d7d 100644 --- a/conf/base.config +++ b/conf/base.config @@ -10,7 +10,6 @@ process { - // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } @@ -24,7 +23,6 @@ process { // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } diff --git a/conf/test_full.config b/conf/test_full.config index 78d70ce3..bf10beb0 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -14,11 +14,8 @@ params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - - // Genome references - genome = 'R64-1-1' + // Parameters for full-size test + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_full.csv' + genome = 'GRCh37' + pseudo_aligner = 'salmon' } diff --git a/docs/output.md b/docs/output.md index f18b6fa6..376cfc01 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,15 +6,34 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [FastQC](#fastqc) - Raw read QC -* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -* [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution +* [Preprocessing](#preprocessing) + * [cat](#cat) - Merge re-sequenced FastQ files + * [FastQC](#fastqc) - Raw read QC +* [Alignment](#alignment) + * [STAR](#star) - Fast spliced aware genome alignment +* [Alignment post-processing](#alignment-post-processing) + * [SAMtools](#samtools) - Sort and index alignments + * [Picard MarkDuplicates](#picard-markduplicates) - Duplicate read marking + * [GATK4 SplitNCigarReads](#gatk4-splitncigarreads) - Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data) + * [GATK4 Base Quality Score Recalibration (BQSR)](#gatk4-bqsr) - Estimate and correct systematic bias that affect the assignment of base quality scores by the sequencer +* [Other steps](#other-steps) + * [Picard BedToIntervalList](#picard-bedtointervallist) - Converts a BED file to a Picard Interval List + * [Picard IntervalListTools](#picard-intervallisttools) - Scatter one interval-list into many interval-files to run analysis in parallel +* [Variant calling](#other-steps) + * [GATK4 HaplotypeCaller](#gatk4-haplotypecaller) - Call SNPs and indels via local re-assembly of haplotypes + * [GATK4 MergeVCFs](#gatk4-mergevcf) - Merge multiple VCF files into one VCF + * [GATK4 IndexFeatureFile](#gatk4-indexfeaturefile) - Index the VCF +* [Variant filtering](#other-steps) + * [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria +* [Quality control](#quality-control) + * [MultiQC](#multiqc) - Present QC for raw reads, alignment, read counting and sample similiarity +* [Workflow reporting and genomes](#workflow-reporting-and-genomes) + * [Reference genome files](#reference-genome-files) - Saving reference genome indices/files + * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ### FastQC diff --git a/docs/usage.md b/docs/usage.md index 00081b8b..62fee01b 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,7 +6,49 @@ ## Introduction - +## Samplesheet input + +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. + +```console +--input '[path to samplesheet file]' +``` + +### Multiple runs of the same sample + +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +``` + +### Full samplesheet + +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. + +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. + +```console +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +``` + +| Column | Description | +|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | + +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. ## Samplesheet input @@ -136,16 +178,16 @@ Specify the path to a specific config file (this is a core Nextflow command). Se ### Resource requests -Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. -For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: +For example, if the nf-core/rnavar pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: ```console -[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' +[62/149eb0] NOTE: Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) +Error executing process > 'RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)' Caused by: - Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) + Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) Command executed: STAR \ @@ -169,7 +211,7 @@ Work dir: Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` ``` -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ```nextflow process { @@ -179,7 +221,7 @@ process { } ``` -> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. +> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNAVAR:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. ### Tool-specific options @@ -187,17 +229,17 @@ For the ultimate flexibility, we have implemented and are using Nextflow DSL2 mo The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: -* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. +* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. -* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. +* An [`options.args`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. -The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. +The nf-core/rnavar pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. -When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. +When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnavar.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnavar.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. -As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. +As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. -Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. +Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. As you will see in the example below, we have: diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 43e5694a..6b3af280 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -9,7 +9,6 @@ class WorkflowMain { // public static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release //"* The pipeline\n" + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + "* The nf-core framework\n" + From 17b783446dd92cf361d50942d5595bed29b605b1 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 19 Nov 2021 12:44:50 +0100 Subject: [PATCH 053/235] fix: usage.md --- docs/usage.md | 81 ++++++++++++++++++++------------------------------- 1 file changed, 31 insertions(+), 50 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 62fee01b..2ecaa5c4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -4,11 +4,9 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ -## Introduction - ## Samplesheet input -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. ```console --input '[path to samplesheet file]' @@ -19,27 +17,27 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: ```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +sample,fastq_1,fastq_2,strandedness +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz,unstranded +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz,unstranded +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz,unstranded ``` ### Full samplesheet -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. ```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +sample,fastq_1,fastq_2,strandedness +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz,forward +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz,forward +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz,forward +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz,,reverse +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz,,reverse +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz,,reverse +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,reverse ``` | Column | Description | @@ -47,59 +45,42 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | | `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `strandedness` | Sample strand-specificity. Must be one of `unstranded`, `forward` or `reverse`. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. -## Samplesheet input - -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +> **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. -```console ---input '[path to samplesheet file]' -``` +## Alignment options -### Multiple runs of the same sample +By default, the pipeline uses [STAR](https://github.com/alexdobin/STAR) (i.e. `--aligner star_salmon`) to map the raw FastQ reads to the reference genome, project the alignments onto the transcriptome and to perform the downstream BAM-level quantification with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html). STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. Since the [RSEM](https://github.com/deweylab/RSEM) (i.e. `--aligner star_rsem`) workflow in the pipeline also uses STAR you should use the [HISAT2](https://ccb.jhu.edu/software/hisat2/index.shtml) aligner (i.e. `--aligner hisat2`) if you have memory limitations. -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: +You also have the option to pseudo-align and quantify your data with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html) by providing the `--pseudo_aligner salmon` parameter. Salmon will then be run in addition to the standard alignment workflow defined by `--aligner`, mainly because it allows you to obtain QC metrics with respect to the genomic alignments. However, you can provide the `--skip_alignment` parameter if you would like to run Salmon in isolation. By default, the pipeline will use the genome fasta and gtf file to generate the transcripts fasta file, and then to build the Salmon index. You can override these parameters using the `--transcript_fasta` and `--salmon_index` parameters, respectively. The library preparation protocol (library type) used by Salmon quantification is inferred by the pipeline based on the information provided in the samplesheet, however, you can override it using the `--salmon_quant_libtype` parameter. You can find the available options in the [Salmon documentation](https://salmon.readthedocs.io/en/latest/library_type.html). -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` +## Reference genome files -### Full samplesheet +The minimum reference genome requirements are a FASTA and GTF file, all other files required to run the pipeline can be generated from these files. However, it is more storage and compute friendly if you are able to re-use reference genome files as efficiently as possible. It is recommended to use the `--save_reference` parameter if you are using the pipeline to build new indices (e.g. those unavailable on [AWS iGenomes](https://nf-co.re/usage/reference_genomes)) so that you can save them somewhere locally. The index building step can be quite a time-consuming process and it permits their reuse for future runs of the pipeline to save disk space. You can then either provide the appropriate reference genome files on the command-line via the appropriate parameters (e.g. `--star_index '/path/to/STAR/index/'`) or via a custom config file. -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +* If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. +* If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. +* If `--gene_bed` is not provided then it will be generated from the GTF file. +* If `--additional_fasta` is provided then the features in this file (e.g. ERCC spike-ins) will be automatically concatenated onto both the reference FASTA file as well as the GTF annotation before building the appropriate indices. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +When using `--aligner star_rsem`, both the STAR and RSEM indices should be present in the path specified by `--rsem_index` (see [#568](https://github.com/nf-core/rnavar/issues/568)) -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, -``` +> **NB:** Compressed reference files are also supported by the pipeline i.e. standard files with the `.gz` extension and indices folders with the `tar.gz` extension. -| Column | Description | -|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +If you are using [GENCODE](https://www.gencodegenes.org/) reference genome files please specify the `--gencode` parameter because the format of these files is slightly different to ENSEMBL genome files: -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. +* The `--gtf_group_features_type` parameter will automatically be set to `gene_type` as opposed to `gene_biotype`, respectively. +* If you are running Salmon, the `--gencode` flag will also be passed to the index building step to overcome parsing issues resulting from the transcript IDs in GENCODE fasta files being separated by vertical pipes (`|`) instead of spaces (see [this issue](https://github.com/COMBINE-lab/salmon/issues/15)). ## Running the pipeline The typical command for running the pipeline is as follows: ```console -nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh37 -profile docker +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. From 2fd9ef9c11765e177cf85bcedb8c983377bc708f Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Fri, 19 Nov 2021 12:46:17 +0100 Subject: [PATCH 054/235] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index e2b5e231..1ac42026 100644 --- a/README.md +++ b/README.md @@ -16,7 +16,7 @@ ## Introduction -**nf-core/rnavar** bioinformatics best-practice pipeline for RNA-Seq variant analysis. +**nf-core/rnavar** is a bioinformatics best-practice pipeline for RNA-Seq variant analysis. The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! From f9f5a6bf010a458fe532a5c8cd05f5b7fc64d530 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 19 Nov 2021 15:28:59 +0100 Subject: [PATCH 055/235] fix: igenomes; naming convention; initialize params --- README.md | 4 ++-- conf/test.config | 9 ++++---- docs/usage.md | 4 +--- lib/WorkflowRnavar.groovy | 11 ++++++++++ main.nf | 17 +++++++++----- nextflow.config | 11 ---------- nextflow_schema.json | 8 +++---- subworkflows/local/prepare_genome.nf | 2 +- workflows/rnavar.nf | 33 ++++++++++++++-------------- 9 files changed, 51 insertions(+), 48 deletions(-) diff --git a/README.md b/README.md index e2b5e231..a1c0ef07 100644 --- a/README.md +++ b/README.md @@ -16,7 +16,7 @@ ## Introduction -**nf-core/rnavar** bioinformatics best-practice pipeline for RNA-Seq variant analysis. +**nf-core/rnavar** is a bioinformatics best-practice pipeline for RNA-Seq variant analysis. The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! @@ -65,7 +65,7 @@ The nf-core/rnavar pipeline comes with documentation about the pipeline [usage]( ## Credits -These scripts were originally written in Nextflow DSL2 for use at the [Barntumörbanken, Karolinska Institutet](https://ki.se/forskning/barntumorbanken) by Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018)) and Maxime U. Garcia ([@maxulysse](https://github.com/maxulysse)). +These scripts were originally written in Nextflow DSL2 for use at the [Barntumörbanken, Karolinska Institutet](https://ki.se/forskning/barntumorbanken), by Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018)) and Maxime U. Garcia ([@maxulysse](https://github.com/maxulysse)). The pipeline is primarily maintained by Praveen Raj ([@praveenraj2018](https://github.com/praveenraj2018)) and Maxime U. Garcia ([@maxulysse](https://github.com/maxulysse)) from [Barntumörbanken, Karolinska Institutet](https://ki.se/forskning/barntumorbanken). diff --git a/conf/test.config b/conf/test.config index c56b48c1..4a998487 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,15 +25,14 @@ params { // Genome references fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' - fasta_dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' + dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' - //gff = 'https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gff.gz' // Known genome resources (optional) - dbsnp_vcf = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' - dbsnp_vcf_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' + dbsnp = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' + dbsnp_tbi = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi' known_indels = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz' - known_indels_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' + known_indels_tbi = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' // STAR index (optional) star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' diff --git a/docs/usage.md b/docs/usage.md index 2ecaa5c4..35aaaafc 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -53,9 +53,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p ## Alignment options -By default, the pipeline uses [STAR](https://github.com/alexdobin/STAR) (i.e. `--aligner star_salmon`) to map the raw FastQ reads to the reference genome, project the alignments onto the transcriptome and to perform the downstream BAM-level quantification with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html). STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. Since the [RSEM](https://github.com/deweylab/RSEM) (i.e. `--aligner star_rsem`) workflow in the pipeline also uses STAR you should use the [HISAT2](https://ccb.jhu.edu/software/hisat2/index.shtml) aligner (i.e. `--aligner hisat2`) if you have memory limitations. - -You also have the option to pseudo-align and quantify your data with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html) by providing the `--pseudo_aligner salmon` parameter. Salmon will then be run in addition to the standard alignment workflow defined by `--aligner`, mainly because it allows you to obtain QC metrics with respect to the genomic alignments. However, you can provide the `--skip_alignment` parameter if you would like to run Salmon in isolation. By default, the pipeline will use the genome fasta and gtf file to generate the transcripts fasta file, and then to build the Salmon index. You can override these parameters using the `--transcript_fasta` and `--salmon_index` parameters, respectively. The library preparation protocol (library type) used by Salmon quantification is inferred by the pipeline based on the information provided in the samplesheet, however, you can override it using the `--salmon_quant_libtype` parameter. You can find the available options in the [Salmon documentation](https://salmon.readthedocs.io/en/latest/library_type.html). +The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome, project the alignments onto the transcriptome and to perform the downstream BAM-level quantification with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html). STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. ## Reference genome files diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 15dd1328..ab5cce51 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -14,6 +14,17 @@ class WorkflowRnavar { log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." System.exit(1) } + + if (!params.gtf && !params.gff) { + log.error "No GTF or GFF3 annotation specified! The pipeline requires at least one of these files." + System.exit(1) + } + + if (!params.dbsnp || !params.dbsnp_tbi) { + log.error "dbSNP VCF file or its index is missing!. Use --dbsnp and --dbsnp_tbi to supply the files." + System.exit(1) + } + } // diff --git a/main.nf b/main.nf index b80e3871..13e201c8 100644 --- a/main.nf +++ b/main.nf @@ -16,12 +16,17 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ======================================================================================== */ - -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') -params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') -params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') -params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fasta_fai = WorkflowMain.getGenomeAttribute(params, 'fasta_fai') +params.dict = WorkflowMain.getGenomeAttribute(params, 'dict') +params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') +params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') +params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') +params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') +params.dbsnp = WorkflowMain.getGenomeAttribute(params, 'dbsnp') +params.dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'dbsnp_tbi') +params.known_indels = WorkflowMain.getGenomeAttribute(params, 'known_indels') +params.known_indels_tbi = WorkflowMain.getGenomeAttribute(params, 'known_indels_tbi') /* ======================================================================================== diff --git a/nextflow.config b/nextflow.config index cdf4a8f2..7885258d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,9 +13,6 @@ params { // Mandatory option input = null // sample sheet - fasta = null - fasta_dict = null - fasta_fai = null // Genome and reference options genome = null @@ -23,14 +20,12 @@ params { igenomes_ignore = false save_reference = false save_merged_fastq = false - gtf = null // Sequence read information read_length = 150 // Required for STAR to build index and align reads // Alignment aligner = 'star' // Only STAR is currently supported. - star_index = null // Provide STAR index if there is already a pre-defined one, or set to null to create one by the pipeline star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR seq_center = null seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work @@ -38,12 +33,6 @@ params { save_unaligned = false save_align_intermeds = false - //Known variant references - known_indels = null - known_indels_index = null - dbsnp_vcf = null - dbsnp_vcf_index = null - // Variant calling no_intervals = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 945d4f88..ebe1b254 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -69,7 +69,7 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, - "fasta_dict": { + "dict": { "type": "string", "fa_icon": "fas fa-file", "description": "Path to FASTA dictionary file.", @@ -125,15 +125,15 @@ "type": "string", "description": "Path to known indels VCF file" }, - "known_indels_index": { + "known_indels_tbi": { "type": "string", "description": "Path to known indels index file" }, - "dbsnp_vcf": { + "dbsnp": { "type": "string", "description": "Path to dbSNP VCF file" }, - "dbsnp_vcf_index": { + "dbsnp_tbi": { "type": "string", "description": "Path to dbSNP VCF index file" } diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 7baea627..2f0aea2f 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -83,7 +83,7 @@ workflow PREPARE_GENOME { // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() - if (params.fasta_dict) ch_fasta_dict = file(params.fasta_dict) + if (params.dict) ch_fasta_dict = file(params.dict) else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict // diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index c6b7c3b8..62765ebd 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -14,11 +14,13 @@ WorkflowRnavar.initialise(params, log) def checkPathParamList = [ params.input, params.fasta, + params.fasta_fai, + params.dict, params.gtf, - params.dbsnp_vcf, - params.dbsnp_vcf_index, + params.dbsnp, + params.dbsnp_tbi, params.known_indels, - params.known_indels_index, + params.known_indels_tbi, params.star_index] for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} @@ -206,6 +208,14 @@ workflow RNAVAR { ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions.first().ifEmpty(null)) + // MODULE: IntervalListTools from GATK4 + ch_interval_list_split = Channel.empty() + if (!params.skip_intervallisttools) { + GATK4_INTERVALLISTTOOLS(ch_interval_list) + ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() + } + else ch_interval_list_split = ch_interval_list + // // SUBWORKFLOW: Alignment with STAR // @@ -254,8 +264,8 @@ workflow RNAVAR { // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() - known_sites = Channel.from([params.dbsnp_vcf, params.known_indels]).collect() - known_sites_tbi = Channel.from([params.dbsnp_vcf_index, params.known_indels_index]).collect() + known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() + known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() @@ -292,15 +302,6 @@ workflow RNAVAR { bam_recalibrated_qc = RECALIBRATE.out.qc ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) - // MODULE: IntervalListTools from GATK4 - ch_interval_list_split = Channel.empty() - - if (!params.skip_intervallisttools) { - GATK4_INTERVALLISTTOOLS(ch_interval_list) - ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() - } - else ch_interval_list_split = ch_interval_list - // MODULE: HaplotypeCaller from GATK4 interval_flag = params.no_intervals haplotypecaller_vcf = Channel.empty() @@ -313,8 +314,8 @@ workflow RNAVAR { GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, - params.dbsnp_vcf, - params.dbsnp_vcf_index, + params.dbsnp, + params.dbsnp_tbi, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, From ef75020faaba16d2e5debcc264329b1b3698fbfb Mon Sep 17 00:00:00 2001 From: "Maxime U. Garcia" Date: Mon, 22 Nov 2021 11:34:49 +0100 Subject: [PATCH 056/235] Update bin/check_samplesheet.py --- bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 91327e5c..96943469 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -7,7 +7,7 @@ def parse_args(args=None): - Description = "Reformat nf-core/rnaseq samplesheet file and check its contents." + Description = "Reformat nf-core/rnavar samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) From abdd649fb174b1851b709656b044936ea6868ef6 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 22 Nov 2021 22:38:13 +0100 Subject: [PATCH 057/235] Added variant annotation subworkflow having snpeff and VEP --- .github/workflows/linting.yml | 2 +- .nf-core.yml | 6 + conf/modules.config | 31 +++++ conf/test.config | 8 ++ main.nf | 26 +++-- modules.json | 11 +- modules/nf-core/modules/ensemblvep/Dockerfile | 30 +++++ modules/nf-core/modules/ensemblvep/build.sh | 27 +++++ .../modules/ensemblvep/environment.yml | 10 ++ .../nf-core/modules/ensemblvep/functions.nf | 78 +++++++++++++ modules/nf-core/modules/ensemblvep/main.nf | 64 +++++++++++ modules/nf-core/modules/ensemblvep/meta.yml | 65 +++++++++++ modules/nf-core/modules/gunzip/main.nf | 10 +- modules/nf-core/modules/gunzip/meta.yml | 6 + modules/nf-core/modules/snpeff/Dockerfile | 22 ++++ modules/nf-core/modules/snpeff/build.sh | 24 ++++ .../nf-core/modules/snpeff/environment.yml | 10 ++ modules/nf-core/modules/snpeff/functions.nf | 78 +++++++++++++ modules/nf-core/modules/snpeff/main.nf | 59 ++++++++++ modules/nf-core/modules/snpeff/meta.yml | 58 ++++++++++ .../modules/tabix/bgziptabix/functions.nf | 78 +++++++++++++ .../nf-core/modules/tabix/bgziptabix/main.nf | 39 +++++++ .../nf-core/modules/tabix/bgziptabix/meta.yml | 45 ++++++++ nextflow.config | 13 +++ nextflow_schema.json | 107 +++++++++++++++++- subworkflows/local/annotate.nf | 84 ++++++++++++++ subworkflows/nf-core/ensemblvep_annotate.nf | 40 +++++++ subworkflows/nf-core/snpeff_annotate.nf | 37 ++++++ workflows/rnavar.nf | 50 +++++++- 29 files changed, 1096 insertions(+), 22 deletions(-) create mode 100644 .nf-core.yml create mode 100644 modules/nf-core/modules/ensemblvep/Dockerfile create mode 100644 modules/nf-core/modules/ensemblvep/build.sh create mode 100644 modules/nf-core/modules/ensemblvep/environment.yml create mode 100644 modules/nf-core/modules/ensemblvep/functions.nf create mode 100644 modules/nf-core/modules/ensemblvep/main.nf create mode 100644 modules/nf-core/modules/ensemblvep/meta.yml create mode 100644 modules/nf-core/modules/snpeff/Dockerfile create mode 100644 modules/nf-core/modules/snpeff/build.sh create mode 100644 modules/nf-core/modules/snpeff/environment.yml create mode 100644 modules/nf-core/modules/snpeff/functions.nf create mode 100644 modules/nf-core/modules/snpeff/main.nf create mode 100644 modules/nf-core/modules/snpeff/meta.yml create mode 100644 modules/nf-core/modules/tabix/bgziptabix/functions.nf create mode 100644 modules/nf-core/modules/tabix/bgziptabix/main.nf create mode 100644 modules/nf-core/modules/tabix/bgziptabix/meta.yml create mode 100644 subworkflows/local/annotate.nf create mode 100644 subworkflows/nf-core/ensemblvep_annotate.nf create mode 100644 subworkflows/nf-core/snpeff_annotate.nf diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 3b448773..c5c9fe38 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -120,7 +120,7 @@ jobs: - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core + pip install nf-core git+https://github.com/nf-core/tools.git@dev - name: Run nf-core lint env: diff --git a/.nf-core.yml b/.nf-core.yml new file mode 100644 index 00000000..fcc940f7 --- /dev/null +++ b/.nf-core.yml @@ -0,0 +1,6 @@ +lint: + files_unchanged: + - .github/workflows/linting.yml + - assets/multiqc_config.yaml + - assets/nf-core-rnavar_logo.png + - docs/images/nf-core-rnavar_logo.png diff --git a/conf/modules.config b/conf/modules.config index 3c32bd82..76452386 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -126,6 +126,37 @@ params { publish_dir = 'variant_filtering' publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] } + // ANNOTATE + 'snpeff' { + args = '-nodownload -canon -v' + publish_files = false + tag_base = '5.0' + } + 'bgziptabix_snpeff' { + publish_dir = 'variant_annotation' + suffix = '_snpEff.ann' + publish_by_meta = true + } + 'merge_vep' { + args = '--everything --filter_common --per_gene --total_length --offline' + publish_files = false + } + 'bgziptabix_merge_vep' { + publish_dir = 'annotation' + suffix = '_snpEff_VEP.ann' + publish_by_meta = true + } + 'ensemblvep' { + args = '--everything --filter_common --per_gene --total_length --offline' + publish_files = false + tag_base = '104.3' + } + 'bgziptabix_vep' { + publish_dir = 'annotation' + suffix = '_VEP.ann' + publish_by_meta = true + } + // QC modules 'fastqc' { args = "--quiet" publish_files = false diff --git a/conf/test.config b/conf/test.config index 4a998487..e7e36593 100644 --- a/conf/test.config +++ b/conf/test.config @@ -27,6 +27,7 @@ params { fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' + gene_bed = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.bed' // Known genome resources (optional) dbsnp = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' @@ -36,4 +37,11 @@ params { // STAR index (optional) star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' + + // Annotation + snpeff_db = 'GRCh38.99' + vep_genome = 'GRCh38.p13' + vep_cache_version = '104' + vep_species = 'homo_sapiens' + } diff --git a/main.nf b/main.nf index 13e201c8..5484abd4 100644 --- a/main.nf +++ b/main.nf @@ -16,17 +16,21 @@ nextflow.enable.dsl = 2 GENOME PARAMETER VALUES ======================================================================================== */ -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.fasta_fai = WorkflowMain.getGenomeAttribute(params, 'fasta_fai') -params.dict = WorkflowMain.getGenomeAttribute(params, 'dict') -params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') -params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') -params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') -params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') -params.dbsnp = WorkflowMain.getGenomeAttribute(params, 'dbsnp') -params.dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'dbsnp_tbi') -params.known_indels = WorkflowMain.getGenomeAttribute(params, 'known_indels') -params.known_indels_tbi = WorkflowMain.getGenomeAttribute(params, 'known_indels_tbi') +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fasta_fai = WorkflowMain.getGenomeAttribute(params, 'fasta_fai') +params.dict = WorkflowMain.getGenomeAttribute(params, 'dict') +params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') +params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') +params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') +params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') +params.dbsnp = WorkflowMain.getGenomeAttribute(params, 'dbsnp') +params.dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'dbsnp_tbi') +params.known_indels = WorkflowMain.getGenomeAttribute(params, 'known_indels') +params.known_indels_tbi = WorkflowMain.getGenomeAttribute(params, 'known_indels_tbi') +params.snpeff_db = WorkflowMain.getGenomeAttribute(params, 'snpeff_db') +params.vep_cache_version = WorkflowMain.getGenomeAttribute(params, 'vep_cache_version') +params.vep_genome = WorkflowMain.getGenomeAttribute(params, 'vep_genome') +params.vep_species = WorkflowMain.getGenomeAttribute(params, 'vep_species') /* ======================================================================================== diff --git a/modules.json b/modules.json index b4bb9975..8d063c6e 100644 --- a/modules.json +++ b/modules.json @@ -9,6 +9,9 @@ "custom/dumpsoftwareversions": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, + "ensemblvep": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, "fastqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, @@ -43,7 +46,7 @@ "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" }, "gunzip": { - "git_sha": "e560fbbc3ed68e62bc810a50f552394056f81762" + "git_sha": "d30bf235b1524327703591c9ce2f372bfb2d474f" }, "multiqc": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" @@ -72,12 +75,18 @@ "samtools/stats": { "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" }, + "snpeff": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, "star/align": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, "star/genomegenerate": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" }, + "tabix/bgziptabix": { + "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + }, "untar": { "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" } diff --git a/modules/nf-core/modules/ensemblvep/Dockerfile b/modules/nf-core/modules/ensemblvep/Dockerfile new file mode 100644 index 00000000..ac1b4691 --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/Dockerfile @@ -0,0 +1,30 @@ +FROM nfcore/base:1.14 +LABEL \ + author="Maxime Garcia" \ + description="VEP image for nf-core pipelines" \ + maintainer="maxime.garcia@scilifelab.se" + +# Install the conda environment +COPY environment.yml / +RUN conda env create -f /environment.yml && conda clean -a + +# Add conda installation dir to PATH (instead of doing 'conda activate') +ENV PATH /opt/conda/envs/nf-core-vep-104.3/bin:$PATH + +# Setup default ARG variables +ARG GENOME=GRCh38 +ARG SPECIES=homo_sapiens +ARG VEP_VERSION=99 + +# Download Genome +RUN vep_install \ + -a c \ + -c .vep \ + -s ${SPECIES} \ + -y ${GENOME} \ + --CACHE_VERSION ${VEP_VERSION} \ + --CONVERT \ + --NO_BIOPERL --NO_HTSLIB --NO_TEST --NO_UPDATE + +# Dump the details of the installed packages to a file for posterity +RUN conda env export --name nf-core-vep-104.3 > nf-core-vep-104.3.yml diff --git a/modules/nf-core/modules/ensemblvep/build.sh b/modules/nf-core/modules/ensemblvep/build.sh new file mode 100644 index 00000000..5fcb91df --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/build.sh @@ -0,0 +1,27 @@ +#!/usr/bin/env bash +set -euo pipefail + +# Build and push all containers + +build_push() { + GENOME=$1 + SPECIES=$2 + VEP_VERSION=$3 + VEP_TAG=$4 + + docker build \ + -t nfcore/vep:${VEP_TAG}.${GENOME} \ + software/vep/. \ + --build-arg GENOME=${GENOME} \ + --build-arg SPECIES=${SPECIES} \ + --build-arg VEP_VERSION=${VEP_VERSION} + + docker push nfcore/vep:${VEP_TAG}.${GENOME} +} + +build_push "GRCh37" "homo_sapiens" "104" "104.3" +build_push "GRCh38" "homo_sapiens" "104" "104.3" +build_push "GRCm38" "mus_musculus" "102" "104.3" +build_push "GRCm39" "mus_musculus" "104" "104.3" +build_push "CanFam3.1" "canis_lupus_familiaris" "104" "104.3" +build_push "WBcel235" "caenorhabditis_elegans" "104" "104.3" diff --git a/modules/nf-core/modules/ensemblvep/environment.yml b/modules/nf-core/modules/ensemblvep/environment.yml new file mode 100644 index 00000000..c0731c26 --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/environment.yml @@ -0,0 +1,10 @@ +# You can use this file to create a conda environment for this module: +# conda env create -f environment.yml +name: nf-core-vep-104.3 +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - bioconda::ensembl-vep=104.3 diff --git a/modules/nf-core/modules/ensemblvep/functions.nf b/modules/nf-core/modules/ensemblvep/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/ensemblvep/main.nf b/modules/nf-core/modules/ensemblvep/main.nf new file mode 100644 index 00000000..ad9c38a6 --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/main.nf @@ -0,0 +1,64 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) +params.use_cache = false +params.vep_tag = "" + +process ENSEMBLVEP { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::ensembl-vep=104.3" : null) + if (params.use_cache) { + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/ensembl-vep:104.3--pl5262h4a94de4_0" + } else { + container "quay.io/biocontainers/ensembl-vep:104.3--pl5262h4a94de4_0" + } + } else { + container "nfcore/vep:${params.vep_tag}" + } + + input: + tuple val(meta), path(vcf) + val genome + val species + val cache_version + path cache + + output: + tuple val(meta), path("*.ann.vcf"), emit: vcf + path "*.summary.html" , emit: report + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + dir_cache = params.use_cache ? "\${PWD}/${cache}" : "/.vep" + """ + mkdir $prefix + + vep \\ + -i $vcf \\ + -o ${prefix}.ann.vcf \\ + $options.args \\ + --assembly $genome \\ + --species $species \\ + --cache \\ + --cache_version $cache_version \\ + --dir_cache $dir_cache \\ + --fork $task.cpus \\ + --format vcf \\ + --stats_file ${prefix}.summary.html + + rm -rf $prefix + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/ensemblvep/meta.yml b/modules/nf-core/modules/ensemblvep/meta.yml new file mode 100644 index 00000000..1b819227 --- /dev/null +++ b/modules/nf-core/modules/ensemblvep/meta.yml @@ -0,0 +1,65 @@ +name: ENSEMBLVEP +description: Ensembl Variant Effect Predictor (VEP) +keywords: + - annotation +tools: + - ensemblvep: + description: | + VEP determines the effect of your variants (SNPs, insertions, deletions, CNVs + or structural variants) on genes, transcripts, and protein sequence, as well as regulatory regions. + homepage: https://www.ensembl.org/info/docs/tools/vep/index.html + documentation: https://www.ensembl.org/info/docs/tools/vep/script/index.html + licence: ['Apache-2.0'] +params: + - use_cache: + type: boolean + description: | + Enable the usage of containers with cache + Does not work with conda + - vep_tag: + type: value + description: | + Specify the tag for the container + https://hub.docker.com/r/nfcore/vep/tags +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + vcf to annotate + - genome: + type: value + description: | + which genome to annotate with + - species: + type: value + description: | + which species to annotate with + - cache_version: + type: value + description: | + which version of the cache to annotate with + - cache: + type: file + description: | + path to VEP cache (optional) +output: + - vcf: + type: file + description: | + annotated vcf + pattern: "*.ann.vcf" + - report: + type: file + description: VEP report file + pattern: "*.html" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf index aec4569f..564fa99d 100644 --- a/modules/nf-core/modules/gunzip/main.nf +++ b/modules/nf-core/modules/gunzip/main.nf @@ -9,7 +9,7 @@ process GUNZIP { label 'process_low' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "conda-forge::sed=4.7" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -19,14 +19,14 @@ process GUNZIP { } input: - path archive + tuple val(meta), path(archive) output: - path "$gunzip", emit: gunzip - path "versions.yml" , emit: versions + tuple val(meta), path("$gunzip"), emit: gunzip + path "versions.yml" , emit: versions script: - gunzip = archive.toString() - '.gz' + gunzip = archive.toString() - '.gz' """ gunzip \\ -f \\ diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml index 3482f0d2..ea1f1546 100644 --- a/modules/nf-core/modules/gunzip/meta.yml +++ b/modules/nf-core/modules/gunzip/meta.yml @@ -10,6 +10,11 @@ tools: documentation: https://www.gnu.org/software/gzip/manual/gzip.html licence: ['GPL-3.0-or-later'] input: + - meta: + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] - archive: type: file description: File to be compressed/uncompressed @@ -26,3 +31,4 @@ output: authors: - "@joseespinosa" - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/modules/snpeff/Dockerfile b/modules/nf-core/modules/snpeff/Dockerfile new file mode 100644 index 00000000..608716a4 --- /dev/null +++ b/modules/nf-core/modules/snpeff/Dockerfile @@ -0,0 +1,22 @@ +FROM nfcore/base:1.14 +LABEL \ + author="Maxime Garcia" \ + description="snpEff image for nf-core pipelines" \ + maintainer="maxime.garcia@scilifelab.se" + +# Install the conda environment +COPY environment.yml / +RUN conda env create -f /environment.yml && conda clean -a + +# Add conda installation dir to PATH (instead of doing 'conda activate') +ENV PATH /opt/conda/envs/nf-core-snpeff-5.0/bin:$PATH + +# Setup default ARG variables +ARG GENOME=GRCh38 +ARG SNPEFF_CACHE_VERSION=99 + +# Download Genome +RUN snpEff download -v ${GENOME}.${SNPEFF_CACHE_VERSION} + +# Dump the details of the installed packages to a file for posterity +RUN conda env export --name nf-core-snpeff-5.0 > nf-core-snpeff-5.0.yml diff --git a/modules/nf-core/modules/snpeff/build.sh b/modules/nf-core/modules/snpeff/build.sh new file mode 100644 index 00000000..b94ffd69 --- /dev/null +++ b/modules/nf-core/modules/snpeff/build.sh @@ -0,0 +1,24 @@ +#!/usr/bin/env bash +set -euo pipefail + +# Build and push all containers + +build_push() { + GENOME=$1 + SNPEFF_CACHE_VERSION=$2 + SNPEFF_TAG=$3 + + docker build \ + -t nfcore/snpeff:${SNPEFF_TAG}.${GENOME} \ + software/snpeff/. \ + --build-arg GENOME=${GENOME} \ + --build-arg SNPEFF_CACHE_VERSION=${SNPEFF_CACHE_VERSION} + + docker push nfcore/snpeff:${SNPEFF_TAG}.${GENOME} +} + +build_push "GRCh37" "75" "5.0" +build_push "GRCh38" "99" "5.0" +build_push "GRCm38" "99" "5.0" +build_push "CanFam3.1" "99" "5.0" +build_push "WBcel235" "99" "5.0" diff --git a/modules/nf-core/modules/snpeff/environment.yml b/modules/nf-core/modules/snpeff/environment.yml new file mode 100644 index 00000000..ad0523fb --- /dev/null +++ b/modules/nf-core/modules/snpeff/environment.yml @@ -0,0 +1,10 @@ +# You can use this file to create a conda environment for this module: +# conda env create -f environment.yml +name: nf-core-snpeff-5.0 +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - bioconda::snpeff=5.0 diff --git a/modules/nf-core/modules/snpeff/functions.nf b/modules/nf-core/modules/snpeff/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/snpeff/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/snpeff/main.nf b/modules/nf-core/modules/snpeff/main.nf new file mode 100644 index 00000000..9652f110 --- /dev/null +++ b/modules/nf-core/modules/snpeff/main.nf @@ -0,0 +1,59 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) +params.use_cache = false +params.snpeff_tag = "" + +process SNPEFF { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? "bioconda::snpeff=5.0" : null) + if (params.use_cache) { + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/snpeff:5.0--hdfd78af_1" + } else { + container "quay.io/biocontainers/snpeff:5.0--hdfd78af_1" + } + } else { + container "nfcore/snpeff:${params.snpeff_tag}" + } + + input: + tuple val(meta), path(vcf), path(vcf_tbi) + val db + path cache + + output: + tuple val(meta), path("*.ann.vcf"), emit: vcf + path "*.csv" , emit: report + path "versions.yml" , emit: versions + + script: + def avail_mem = 6 + if (!task.memory) { + log.info '[snpEff] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + cache = params.use_cache ? "-dataDir \${PWD}/${snpeff_cache}" : "" + """ + snpEff -Xmx${avail_mem}g \\ + $db \\ + $options.args \\ + -csvStats ${prefix}.csv \\ + $cache \\ + $vcf \\ + > ${prefix}.ann.vcf + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(snpEff -version 2>&1) | cut -f 2 -d ' ') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/snpeff/meta.yml b/modules/nf-core/modules/snpeff/meta.yml new file mode 100644 index 00000000..8ba18683 --- /dev/null +++ b/modules/nf-core/modules/snpeff/meta.yml @@ -0,0 +1,58 @@ +name: snpEff +description: Genetic variant annotation and functional effect prediction toolbox +keywords: + - annotation +tools: + - snpeff: + description: | + SnpEff is a variant annotation and effect prediction tool. + It annotates and predicts the effects of genetic variants on genes and proteins (such as amino acid changes). + homepage: https://pcingola.github.io/SnpEff/ + documentation: https://pcingola.github.io/SnpEff/se_introduction/ + licence: ['MIT'] +params: + - use_cache: + type: boolean + description: | + boolean to enable the usage of containers with cache + Enable the usage of containers with cache + Does not work with conda + - snpeff_tag: + type: value + description: | + Specify the tag for the container + https://hub.docker.com/r/nfcore/snpeff/tags +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + vcf to annotate + - db: + type: value + description: | + which db to annotate with + - cache: + type: file + description: | + path to snpEff cache (optional) +output: + - vcf: + type: file + description: | + annotated vcf + pattern: "*.ann.vcf" + - report: + type: file + description: snpEff report file + pattern: "*.html" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" diff --git a/modules/nf-core/modules/tabix/bgziptabix/functions.nf b/modules/nf-core/modules/tabix/bgziptabix/functions.nf new file mode 100644 index 00000000..85628ee0 --- /dev/null +++ b/modules/nf-core/modules/tabix/bgziptabix/functions.nf @@ -0,0 +1,78 @@ +// +// Utility functions used in nf-core DSL2 module files +// + +// +// Extract name of software tool from process name using $task.process +// +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +// +// Extract name of module from process name using $task.process +// +def getProcessName(task_process) { + return task_process.tokenize(':')[-1] +} + +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +// +// Tidy up and join elements of a list to return a path string +// +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +// +// Function to save/publish module results +// +def saveFiles(Map args) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + + // Do not publish versions.yml unless running from pytest workflow + if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { + return null + } + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } +} diff --git a/modules/nf-core/modules/tabix/bgziptabix/main.nf b/modules/nf-core/modules/tabix/bgziptabix/main.nf new file mode 100644 index 00000000..e44a7226 --- /dev/null +++ b/modules/nf-core/modules/tabix/bgziptabix/main.nf @@ -0,0 +1,39 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process TABIX_BGZIPTABIX { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::tabix=1.11' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0" + } else { + container "quay.io/biocontainers/tabix:1.11--hdfd78af_0" + } + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.gz"), path("*.tbi"), emit: tbi + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + bgzip -c $options.args $input > ${prefix}.gz + tabix $options.args2 ${prefix}.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/tabix/bgziptabix/meta.yml b/modules/nf-core/modules/tabix/bgziptabix/meta.yml new file mode 100644 index 00000000..f2aed84d --- /dev/null +++ b/modules/nf-core/modules/tabix/bgziptabix/meta.yml @@ -0,0 +1,45 @@ +name: tabix_bgziptabix +description: bgzip a sorted tab-delimited genome file and then create tabix index +keywords: + - bgzip + - compress + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file + pattern: "*.{bed,gff,sam,vcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gz: + type: file + description: Output compressed file + pattern: "*.{gz}" + - tbi: + type: file + description: tabix index file + pattern: "*.{gz.tbi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" diff --git a/nextflow.config b/nextflow.config index 7885258d..a9282764 100644 --- a/nextflow.config +++ b/nextflow.config @@ -36,9 +36,22 @@ params { // Variant calling no_intervals = false + // Variant annotation + annotate_tools = 'snpeff' // List of annotation tools to run - snpeff or vep or merge + annotation_cache = false // Annotation cache disabled + cadd_cache = null // CADD cache disabled + cadd_indels = false // No CADD InDels file + cadd_indels_tbi = false // No CADD InDels index + cadd_wg_snvs = false // No CADD SNVs file + cadd_wg_snvs_tbi = false // No CADD SNVs index + genesplicer = null // genesplicer disabled within VEP + snpeff_cache = null // No directory for snpEff cache + vep_cache = null // No directory for VEP cache + // Skip steps skip_intervallisttools = false skip_variantfiltration = false + skip_variantannotation = false // GATK intervallist parameters scatter_count = 6 diff --git a/nextflow_schema.json b/nextflow_schema.json index ebe1b254..cf9ab633 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -136,6 +136,28 @@ "dbsnp_tbi": { "type": "string", "description": "Path to dbSNP VCF index file" + }, + "snpeff_db": { + "type": "string", + "fa_icon": "fas fa-database", + "description": "snpEff DB version" + }, + "vep_genome": { + "type": "string", + "fa_icon": "fas fa-microscope", + "description": "VEP genome", + "help_text": "If you use AWS iGenomes or a local resource with genomes.conf, this has already been set for you appropriately." + }, + "vep_species": { + "type": "string", + "fa_icon": "fas fa-microscope", + "description": "VEP species", + "help_text": "If you use AWS iGenomes or a local resource with genomes.conf, this has already been set for you appropriately." + }, + "vep_cache_version": { + "type": "string", + "fa_icon": "fas fa-tag", + "description": "VEP cache version" } } }, @@ -188,6 +210,80 @@ } } }, + "annotation": { + "title": "Annotation", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-toolbox", + "properties": { + "annotate_tools": { + "type": "string", + "fa_icon": "fas fa-hammer", + "description": "Specify which tools RNAvar should use for annotating variants. Values can be 'snpeff', 'vep' or 'merge'. If you specify 'merge', the pipeline runs both snpeff and VEP annotation.", + "help_text": "List of tools to be used for variant annotation.", + "pattern": "^((snpeff|vep|merge)*(,)*)*$", + "hidden": true + }, + "annotation_cache": { + "type": "boolean", + "fa_icon": "fas fa-database", + "description": "Enable the use of cache for annotation", + "help_text": "And disable usage of snpeff and vep specific containers for annotation\n\nTo be used with `--snpeff_cache` and/or `--vep_cache`", + "hidden": true + }, + "cadd_cache": { + "type": "boolean", + "fa_icon": "fas fa-database", + "description": "Enable CADD cache.", + "hidden": true + }, + "cadd_indels": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to CADD InDels file.", + "hidden": true + }, + "cadd_indels_tbi": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to CADD InDels index.", + "hidden": true + }, + "cadd_wg_snvs": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to CADD SNVs file.", + "hidden": true + }, + "cadd_wg_snvs_tbi": { + "type": "string", + "fa_icon": "fas fa-file", + "description": "Path to CADD SNVs index.", + "hidden": true + }, + "genesplicer": { + "type": "boolean", + "fa_icon": "fas fa-gavel", + "description": "Enable the use of the VEP GeneSplicer plugin.", + "hidden": true + }, + "snpeff_cache": { + "type": "string", + "fa_icon": "fas fa-database", + "description": "Path to snpEff cache", + "help_text": "To be used with `--annotation_cache`", + "hidden": true + }, + "vep_cache": { + "type": "string", + "fa_icon": "fas fa-database", + "description": "Path to VEP cache", + "help_text": "To be used with `--annotation_cache`", + "hidden": true + } + } + }, "pipeline_stage_options": { "title": "Pipeline stage options", "type": "object", @@ -202,7 +298,12 @@ "skip_variantfiltration": { "type": "string", "description": "Skip variant filtering of GATK", - "help_text": "Set this parameter if you don't want to filter any variants. " + "help_text": "Set this parameter if you don't want to filter any variants." + }, + "skip_variantannotation": { + "type": "string", + "description": "Skip variant annotation", + "help_text": "Set this parameter if you don't want to run variant annotation." }, "skip_qc": { "type": "string", @@ -466,6 +567,10 @@ { "$ref": "#/definitions/alignment_options" } + , + { + "$ref": "#/definitions/annotation" + } ], "required": [ "aligner", diff --git a/subworkflows/local/annotate.nf b/subworkflows/local/annotate.nf new file mode 100644 index 00000000..ff5b76b1 --- /dev/null +++ b/subworkflows/local/annotate.nf @@ -0,0 +1,84 @@ +// +// ANNOTATION +// + +params.annotation_cache = [:] +params.bgziptabix_merge_vep_options = [:] +params.bgziptabix_snpeff_options = [:] +params.bgziptabix_vep_options = [:] +params.merge_vep_options = [:] +params.snpeff_options = [:] +params.snpeff_tag = [:] +params.vep_options = [:] +params.vep_tag = [:] + +include { SNPEFF_ANNOTATE } from '../nf-core/snpeff_annotate' addParams( + bgziptabix_snpeff_options: params.bgziptabix_snpeff_options, + snpeff_options: params.snpeff_options, + snpeff_tag: params.snpeff_tag, + use_cache: params.annotation_cache +) + +include { ENSEMBLVEP_ANNOTATE as MERGE_ANNOTATE } from '../nf-core/ensemblvep_annotate' addParams( + bgziptabix_vep_options: params.bgziptabix_merge_vep_options, + use_cache: params.annotation_cache, + vep_options: params.merge_vep_options, + vep_tag: params.vep_tag +) + +include { ENSEMBLVEP_ANNOTATE } from '../nf-core/ensemblvep_annotate' addParams( + bgziptabix_vep_options: params.bgziptabix_vep_options, + use_cache: params.annotation_cache, + vep_options: params.vep_options, + vep_tag: params.vep_tag +) + +workflow ANNOTATE { + take: + vcf // channel: [ val(meta), vcf ] + tools + snpeff_db + snpeff_cache + vep_genome + vep_species + vep_cache_version + vep_cache + + main: + + ch_versions = Channel.empty() + merge_vcf_ann = Channel.empty() + merge_vep_report = Channel.empty() + merge_vep_version = Channel.empty() + snpeff_report = Channel.empty() + snpeff_vcf_ann = Channel.empty() + snpeff_version = Channel.empty() + vep_report = Channel.empty() + vep_vcf_ann = Channel.empty() + vep_version = Channel.empty() + + if ('snpeff' in tools || 'merge' in tools) { + (snpeff_vcf_ann, snpeff_report, snpeff_version) = SNPEFF_ANNOTATE(vcf, snpeff_db, snpeff_cache) + ch_versions = ch_versions.mix(SNPEFF_ANNOTATE.out.versions.first()) + } + + if ('merge' in tools) { + vcf_ann_for_merge = snpeff_vcf_ann.map{ meta, vcf, tbi -> [meta, vcf] } + (merge_vcf_ann, merge_vep_report, merge_vep_version) = MERGE_ANNOTATE(vcf_ann_for_merge, vep_genome, vep_species, vep_cache_version, vep_cache) + ch_versions = ch_versions.mix(MERGE_ANNOTATE.out.versions.first()) + } + + if ('vep' in tools) { + (vep_vcf_ann, vep_report, vep_version) = ENSEMBLVEP_ANNOTATE(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + ch_versions = ch_versions.mix(ENSEMBLVEP_ANNOTATE.out.versions.first()) + } + + vcf_ann = snpeff_vcf_ann.mix(merge_vcf_ann, vep_vcf_ann) + reports = snpeff_report.mix(merge_vep_report, vep_report) + versions = ch_versions + + emit: + reports + vcf_ann + versions +} diff --git a/subworkflows/nf-core/ensemblvep_annotate.nf b/subworkflows/nf-core/ensemblvep_annotate.nf new file mode 100644 index 00000000..b1b6725d --- /dev/null +++ b/subworkflows/nf-core/ensemblvep_annotate.nf @@ -0,0 +1,40 @@ +// +// Run VEP to annotate VCF files +// + +params.bgziptabix_vep = [:] +params.vep_options = [:] +params.vep_tag = [:] +params.use_cache = [:] + +include { ENSEMBLVEP } from '../../modules/nf-core/modules/ensemblvep/main' addParams( + options: params.vep_options, + use_cache: params.use_cache, + vep_tag: params.vep_tag +) + +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' addParams(options: params.bgziptabix_vep_options) + +workflow ENSEMBLVEP_ANNOTATE { + take: + vcf // channel: [ val(meta), vcf, tbi ] + vep_genome // value: which genome + vep_species // value: which species + vep_cache_version // value: which cache version + vep_cache // path: path_to_vep_cache (optionnal) + // skip // boolean: true/false + + main: + + ch_versions = Channel.empty() + + ENSEMBLVEP(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions.first()) + TABIX_BGZIPTABIX(ENSEMBLVEP.out.vcf) + ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) + + emit: + vcf = TABIX_BGZIPTABIX.out.tbi // channel: [ val(meta), vcf, tbi ] + vep_report = ENSEMBLVEP.out.report // path: *.html + versions = ch_versions // channel: [versions.yml] +} diff --git a/subworkflows/nf-core/snpeff_annotate.nf b/subworkflows/nf-core/snpeff_annotate.nf new file mode 100644 index 00000000..4cef6187 --- /dev/null +++ b/subworkflows/nf-core/snpeff_annotate.nf @@ -0,0 +1,37 @@ +// +// Run snpEff to annotate VCF files +// + +params.bgziptabix_snpeff = [:] +params.snpeff_options = [:] +params.snpeff_tag = [:] +params.use_cache = [:] + +include { SNPEFF } from '../../modules/nf-core/modules/snpeff/main' addParams( + options: params.snpeff_options, + snpeff_tag: params.snpeff_tag, + use_cache: params.use_cache +) + +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' addParams(options: params.bgziptabix_snpeff_options) + +workflow SNPEFF_ANNOTATE { + take: + vcf // channel: [ val(meta), vcf, tbi ] + snpeff_db // value: version of db to use + snpeff_cache // path: path_to_snpeff_cache (optionnal) + + main: + + ch_versions = Channel.empty() + + SNPEFF(vcf, snpeff_db, snpeff_cache) + ch_versions = ch_versions.mix(SNPEFF.out.versions.first()) + TABIX_BGZIPTABIX(SNPEFF.out.vcf) + ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) + + emit: + vcf = TABIX_BGZIPTABIX.out.tbi // channel: [ val(meta), vcf, tbi ] + snpeff_report = SNPEFF.out.report // path: *.html + versions = ch_versions // channel: [versions.yml] +} diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 62765ebd..80e50bc0 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -21,6 +21,8 @@ def checkPathParamList = [ params.dbsnp_tbi, params.known_indels, params.known_indels_tbi, + params.snpeff_cache, + params.vep_cache, params.star_index] for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} @@ -107,6 +109,16 @@ if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) +// Initialize varaint annotation associated channels +def tools = params.annotate_tools ? params.annotate_tools.split(',').collect{it.trim().toLowerCase().replaceAll('-', '').replaceAll('_', '')} : [] +def snpeff_db = params.snpeff_db ?: Channel.empty() +def vep_cache_version = params.vep_cache_version ?: Channel.empty() +def vep_genome = params.vep_genome ?: Channel.empty() +def vep_species = params.vep_species ?: Channel.empty() +def snpeff_cache = params.snpeff_cache ? params.snpeff_cache : [] +def vep_cache = params.vep_cache ? params.vep_cache : [] + +// Include all the modules required for the pipeline include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) @@ -117,12 +129,16 @@ include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/index include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) +// Include all the subworkflows required for the pipeline + +// Build the genome index and other reference files include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( genome_options: publish_genome_options, index_options: publish_index_options, star_index_options: modules['star_genomegenerate'], star_untar_options: untar_options ) +// Align reads to genome and sort and index the alignment file include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( align_options: modules['star_align'], samtools_index_options: modules['samtools_index_genome'], @@ -130,21 +146,36 @@ include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' samtools_stats_options: modules['samtools_index_genome'], seq_platform: params.seq_platform ) +// Mark duplicates in the BAM file include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( markduplicates_options: modules['picard_markduplicates'], samtools_index_options: modules['picard_markduplicates_samtools'], samtools_stats_options: modules['picard_markduplicates_samtools'] ) +// Splits reads that contain Ns in their cigar string include { GATK4_SPLITNCIGAR } from '../subworkflows/nf-core/splitn_cigar_reads' addParams( gatk_splitncigar_options: modules['gatk_splitncigar_options'], samtools_index_options: modules['picard_markduplicates_samtools'] ) +// Estimate and correct systematic bias include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( applybqsr_options: modules['applybqsr'], merge_bam_options: modules['merge_bam_recalibrate'], samtools_index_options: modules['samtools_index_recalibrate'], samtools_stats_options: modules['samtools_stats_recalibrate'] ) +// Annotate variants using snpEff or VEP or both +include { ANNOTATE } from '../subworkflows/local/annotate' addParams( + annotation_cache: params.annotation_cache, + bgziptabix_merge_vep_options: modules['bgziptabix_merge_vep'], + bgziptabix_snpeff_options: modules['bgziptabix_snpeff'], + bgziptabix_vep_options: modules['bgziptabix_vep'], + merge_vep_options: modules['merge_vep'], + snpeff_options: modules['snpeff'], + snpeff_tag: "${modules['snpeff'].tag_base}.${params.genome}", + vep_options: modules['ensemblvep'], + vep_tag: "${modules['ensemblvep'].tag_base}.${params.genome}" +) // Info required for completion email and summary def multiqc_report = [] @@ -344,6 +375,7 @@ workflow RNAVAR { ) haplotypecaller_vcf_tbi = haplotypecaller_vcf.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) + final_vcf_tbi = haplotypecaller_vcf_tbi // MODULE: VariantFiltration from GATK4 if (!params.skip_variantfiltration) { @@ -355,9 +387,21 @@ workflow RNAVAR { PREPARE_GENOME.out.dict ) - filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf - filtered_vcf_tbi = GATK4_VARIANTFILTRATION.out.tbi - ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) + filtered_vcf_tbi = GATK4_VARIANTFILTRATION.out.vcf.join(GATK4_VARIANTFILTRATION.out.tbi, by: [0]) + final_vcf_tbi = filtered_vcf_tbi + ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) + } + + if(!params.skip_variantannotation && ('merge' in tools || 'snpeff' in tools || 'vep' in tools)) { + ANNOTATE( + final_vcf_tbi, + tools, + snpeff_db, + snpeff_cache, + vep_genome, + vep_species, + vep_cache_version, + vep_cache) } } From 863a90944ff4638c96c0dcd031f89f0afdb279f8 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 22 Nov 2021 22:50:32 +0100 Subject: [PATCH 058/235] Added variant annotation subworkflow having snpeff and VEP --- nextflow_schema.json | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index cf9ab633..488cd48a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -210,8 +210,8 @@ } } }, - "annotation": { - "title": "Annotation", + "variant_annotation": { + "title": "Variant Annotation", "type": "object", "description": "", "default": "", @@ -569,7 +569,7 @@ } , { - "$ref": "#/definitions/annotation" + "$ref": "#/definitions/variant_annotation" } ], "required": [ From 315420fc33de53834634dd7031a7c2f2f5596ccf Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 22 Nov 2021 23:30:41 +0100 Subject: [PATCH 059/235] params.genome parameter added in test.config --- conf/test.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/test.config b/conf/test.config index e7e36593..e6dc4148 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,6 +23,7 @@ params { input = 'assets/samplesheet_test.csv' // Genome references + genome = 'GRCh38' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' From 77ac8779e235bd7c11f5925021d6654e81fc07bb Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 22 Nov 2021 23:37:27 +0100 Subject: [PATCH 060/235] fix: capture ch_versions from annotate sub-workflow --- conf/modules.config | 4 ++-- workflows/rnavar.nf | 1 + 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 76452386..bbb9e3cb 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -142,7 +142,7 @@ params { publish_files = false } 'bgziptabix_merge_vep' { - publish_dir = 'annotation' + publish_dir = 'variant_annotation' suffix = '_snpEff_VEP.ann' publish_by_meta = true } @@ -152,7 +152,7 @@ params { tag_base = '104.3' } 'bgziptabix_vep' { - publish_dir = 'annotation' + publish_dir = 'variant_annotation' suffix = '_VEP.ann' publish_by_meta = true } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 80e50bc0..226d436e 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -402,6 +402,7 @@ workflow RNAVAR { vep_species, vep_cache_version, vep_cache) + ch_versions = ch_versions.mix(ANNOTATE.out.versions.first().ifEmpty(null)) } } From 5b6713fa4f049b940b55ab68fd07a3d3a005276e Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 24 Nov 2021 23:32:42 +0100 Subject: [PATCH 061/235] update: snp and vep genome dataases in test.config --- conf/test.config | 8 ++++---- nextflow.config | 2 +- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/test.config b/conf/test.config index e6dc4148..f9ca0800 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,7 +23,7 @@ params { input = 'assets/samplesheet_test.csv' // Genome references - genome = 'GRCh38' + genome = 'WBcel235' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' @@ -40,9 +40,9 @@ params { star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' // Annotation - snpeff_db = 'GRCh38.99' - vep_genome = 'GRCh38.p13' + snpeff_db = 'WBcel235.99' + vep_genome = 'WBcel235' + vep_species = 'caenorhabditis_elegans' vep_cache_version = '104' - vep_species = 'homo_sapiens' } diff --git a/nextflow.config b/nextflow.config index a9282764..e021dfac 100644 --- a/nextflow.config +++ b/nextflow.config @@ -37,7 +37,7 @@ params { no_intervals = false // Variant annotation - annotate_tools = 'snpeff' // List of annotation tools to run - snpeff or vep or merge + annotate_tools = 'merge' // List of annotation tools to run - snpeff or vep or merge annotation_cache = false // Annotation cache disabled cadd_cache = null // CADD cache disabled cadd_indels = false // No CADD InDels file From 3f8e54db72d01c27f71f05451ed38ae6d06ee730 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 24 Nov 2021 23:52:40 +0100 Subject: [PATCH 062/235] fix: lint errors --- .github/ISSUE_TEMPLATE/bug_report.md | 63 ---------------------- .github/ISSUE_TEMPLATE/bug_report.yml | 52 ++++++++++++++++++ .github/ISSUE_TEMPLATE/config.yml | 1 - .github/ISSUE_TEMPLATE/feature_request.md | 32 ----------- .github/ISSUE_TEMPLATE/feature_request.yml | 11 ++++ lib/NfcoreSchema.groovy | 19 +++++-- 6 files changed, 79 insertions(+), 99 deletions(-) delete mode 100644 .github/ISSUE_TEMPLATE/bug_report.md create mode 100644 .github/ISSUE_TEMPLATE/bug_report.yml delete mode 100644 .github/ISSUE_TEMPLATE/feature_request.md create mode 100644 .github/ISSUE_TEMPLATE/feature_request.yml diff --git a/.github/ISSUE_TEMPLATE/bug_report.md b/.github/ISSUE_TEMPLATE/bug_report.md deleted file mode 100644 index bd5117f0..00000000 --- a/.github/ISSUE_TEMPLATE/bug_report.md +++ /dev/null @@ -1,63 +0,0 @@ ---- -name: Bug report -about: Report something that is broken or incorrect -labels: bug ---- - - - -## Check Documentation - -I have checked the following places for your error: - -- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) -- [ ] [nf-core/rnavar pipeline documentation](https://nf-co.re/rnavar/usage) - -## Description of the bug - - - -## Steps to reproduce - -Steps to reproduce the behaviour: - -1. Command line: -2. See error: - -## Expected behaviour - - - -## Log files - -Have you provided the following extra information/files: - -- [ ] The command used to run the pipeline -- [ ] The `.nextflow.log` file - -## System - -- Hardware: -- Executor: -- OS: -- Version - -## Nextflow Installation - -- Version: - -## Container engine - -- Engine: -- version: - -## Additional context - - diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml new file mode 100644 index 00000000..358b415f --- /dev/null +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -0,0 +1,52 @@ + +name: Bug report +description: Report something that is broken or incorrect +labels: bug +body: + + - type: markdown + attributes: + value: | + Before you post this issue, please check the documentation: + + - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) + - [nf-core/sarek pipeline documentation](https://nf-co.re/sarek/usage) + + - type: textarea + id: description + attributes: + label: Description of the bug + description: A clear and concise description of what the bug is. + validations: + required: true + + - type: textarea + id: command_used + attributes: + label: Command used and terminal output + description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal. + render: console + placeholder: | + $ nextflow run ... + + Some output where something broke + + - type: textarea + id: files + attributes: + label: Relevant files + description: | + Please upload (drag and drop) and relevant files. Make into a `.zip` file if the extension is not allowed. + Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files. + + - type: textarea + id: system + attributes: + label: System information + description: | + * Nextflow version _(eg. 21.04.01)_ + * Hardware _(eg. HPC, Desktop, Cloud)_ + * Executor _(eg. slurm, local, awsbatch)_ + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ + * OS _(eg. CentOS Linux, macOS, Linux Mint)_ + * Version of nf-core/sarek _(eg. 1.1, 1.5, 1.8.2)_ diff --git a/.github/ISSUE_TEMPLATE/config.yml b/.github/ISSUE_TEMPLATE/config.yml index 1504ac9b..066da18a 100644 --- a/.github/ISSUE_TEMPLATE/config.yml +++ b/.github/ISSUE_TEMPLATE/config.yml @@ -1,4 +1,3 @@ -blank_issues_enabled: false contact_links: - name: Join nf-core url: https://nf-co.re/join diff --git a/.github/ISSUE_TEMPLATE/feature_request.md b/.github/ISSUE_TEMPLATE/feature_request.md deleted file mode 100644 index a0198f2b..00000000 --- a/.github/ISSUE_TEMPLATE/feature_request.md +++ /dev/null @@ -1,32 +0,0 @@ ---- -name: Feature request -about: Suggest an idea for the nf-core/rnavar pipeline -labels: enhancement ---- - - - -## Is your feature request related to a problem? Please describe - - - - - -## Describe the solution you'd like - - - -## Describe alternatives you've considered - - - -## Additional context - - diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml new file mode 100644 index 00000000..604195a0 --- /dev/null +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -0,0 +1,11 @@ +name: Feature request +description: Suggest an idea for the nf-core/sarek pipeline +labels: enhancement +body: + - type: textarea + id: description + attributes: + label: Description of feature + description: Please describe your suggestion for a new feature. It might help to describe a problem or use case, plus any alternatives that you have considered. + validations: + required: true diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index dcb39c83..5f681078 100755 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -105,9 +105,13 @@ class NfcoreSchema { // Collect expected parameters from the schema def expectedParams = [] + def enums = [:] for (group in schemaParams) { for (p in group.value['properties']) { expectedParams.push(p.key) + if (group.value['properties'][p.key].containsKey('enum')) { + enums[p.key] = group.value['properties'][p.key]['enum'] + } } } @@ -155,7 +159,7 @@ class NfcoreSchema { println '' log.error 'ERROR: Validation of pipeline parameters failed!' JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log) + printExceptions(exceptionJSON, params_json, log, enums) println '' has_error = true } @@ -329,7 +333,7 @@ class NfcoreSchema { // // Loop over nested exceptions and print the causingException // - private static void printExceptions(ex_json, params_json, log) { + private static void printExceptions(ex_json, params_json, log, enums, limit=5) { def causingExceptions = ex_json['causingExceptions'] if (causingExceptions.length() == 0) { def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ @@ -345,7 +349,16 @@ class NfcoreSchema { else { def param = ex_json['pointerToViolation'] - ~/^#\// def param_val = params_json[param].toString() - log.error "* --${param}: ${ex_json['message']} (${param_val})" + if (enums.containsKey(param)) { + def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" + if (enums[param].size() > limit) { + log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" + } else { + log.error "${error_msg}: ${enums[param].join(', ')})" + } + } else { + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } } } for (ex in causingExceptions) { From cb92cc6f8cfc8e454d4ec38ba866b34b008b3a4b Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 25 Nov 2021 00:12:56 +0100 Subject: [PATCH 063/235] fix: lint errors on issue templates --- .github/ISSUE_TEMPLATE/bug_report.yml | 4 ++-- .github/ISSUE_TEMPLATE/feature_request.yml | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 358b415f..200eb840 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -10,7 +10,7 @@ body: Before you post this issue, please check the documentation: - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) - - [nf-core/sarek pipeline documentation](https://nf-co.re/sarek/usage) + - [nf-core/rnavar pipeline documentation](https://nf-co.re/rnavar/usage) - type: textarea id: description @@ -49,4 +49,4 @@ body: * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ * OS _(eg. CentOS Linux, macOS, Linux Mint)_ - * Version of nf-core/sarek _(eg. 1.1, 1.5, 1.8.2)_ + * Version of nf-core/rnavar _(eg. 1.1, 1.5, 1.8.2)_ diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml index 604195a0..9a0750e4 100644 --- a/.github/ISSUE_TEMPLATE/feature_request.yml +++ b/.github/ISSUE_TEMPLATE/feature_request.yml @@ -1,5 +1,5 @@ name: Feature request -description: Suggest an idea for the nf-core/sarek pipeline +description: Suggest an idea for the nf-core/rnavar pipeline labels: enhancement body: - type: textarea From a20f0ae3a164e42676300a5d013286d5bd58fb5a Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 25 Nov 2021 06:45:37 +0100 Subject: [PATCH 064/235] Added parameters for remove_duplicates and star_twopass --- conf/modules.config | 4 ++-- nextflow.config | 4 ++++ nextflow_schema.json | 36 ++++++++++++++++++++++++++++-------- workflows/rnavar.nf | 2 ++ 4 files changed, 36 insertions(+), 10 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index bbb9e3cb..93b3bdee 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -30,7 +30,7 @@ params { publish_dir = 'genome/index' } 'star_align' { - args = "--twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" + args = "--outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" publish_files = false } 'samtools_sort_genome' { @@ -43,7 +43,7 @@ params { publish_files = false } 'picard_markduplicates' { - args = 'ASSUME_SORTED=true REMOVE_DUPLICATES=false VALIDATION_STRINGENCY=LENIENT TMP_DIR=tmp' + args = 'ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT' suffix = '.markdup.sorted' publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] publish_dir = "preprocessing" diff --git a/nextflow.config b/nextflow.config index e021dfac..33b56492 100644 --- a/nextflow.config +++ b/nextflow.config @@ -26,6 +26,7 @@ params { // Alignment aligner = 'star' // Only STAR is currently supported. + star_twopass = true star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR seq_center = null seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work @@ -33,6 +34,9 @@ params { save_unaligned = false save_align_intermeds = false + // Preprocessing of alignment + remove_duplicates = false + // Variant calling no_intervals = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 488cd48a..a046bacf 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -178,6 +178,11 @@ "description": "Path to STAR index folder or compressed file (tar.gz)", "help_text": "This parameter can be used if there is an pre-defined STAR index available. You can either give the full path to the index directory or a compressed file in tar.gz format." }, + "star_twopass": { + "type": "boolean", + "description": "Enable STAR 2-pass mapping mode.", + "help_text": "This parameter enables STAR to perform 2-pass mapping. Default true." + }, "star_ignore_sjdbgtf": { "type": "string", "description": "Do not use GTF file during STAR index buidling step", @@ -210,6 +215,22 @@ } } }, + "preprocessing": { + "title": "Preprocessing of alignment", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-toolbox", + "properties": { + "remove_duplicates": { + "type": "boolean", + "fa_icon": "fas fa-hammer", + "description": "Specify whether to remove duplicates from the BAM during Picard MarkDuplicates step.", + "help_text": "Specify true for removing duplicates from BAM file during Picard MarkDuplicates step.", + "hidden": false + } + } + }, "variant_annotation": { "title": "Variant Annotation", "type": "object", @@ -554,22 +575,21 @@ }, { "$ref": "#/definitions/generic_options" - } - , + }, { "$ref": "#/definitions/tool_parameters" - } - , + }, { "$ref": "#/definitions/pipeline_stage_options" - } - , + }, { "$ref": "#/definitions/alignment_options" - } - , + }, { "$ref": "#/definitions/variant_annotation" + }, + { + "$ref": "#/definitions/preprocessing" } ], "required": [ diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 226d436e..95342adb 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -98,10 +98,12 @@ if (params.save_align_intermeds) { if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +modules['star_align'].args += params.star_twopass ? Utils.joinModuleArgs(['--twopassMode Basic']) : '' if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +modules['picard_markduplicates'].args += params.remove_duplicates ? Utils.joinModuleArgs(['REMOVE_DUPLICATES=true']) : '' modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) From 868ed214cd97d43f05ff7c53e769ff19fa6035dc Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 11:05:35 +0100 Subject: [PATCH 065/235] Update: parelle process for splitNcigar --- conf/modules.config | 4 ++ .../gatk4/splitncigarreads/functions.nf | 0 .../gatk4/splitncigarreads/main.nf | 4 +- .../gatk4/splitncigarreads/meta.yml | 0 subworkflows/local/preprocess.nf | 56 +++++++++++++++++++ subworkflows/nf-core/splitn_cigar_reads.nf | 12 ++-- workflows/rnavar.nf | 22 ++++---- 7 files changed, 81 insertions(+), 17 deletions(-) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/functions.nf (100%) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/main.nf (90%) rename modules/{nf-core/modules => local}/gatk4/splitncigarreads/meta.yml (100%) create mode 100644 subworkflows/local/preprocess.nf diff --git a/conf/modules.config b/conf/modules.config index 93b3bdee..ed4a34af 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -37,6 +37,10 @@ params { suffix = '.sorted' publish_files = false } + 'samtools_merge_genome' { + suffix = '.merged' + publish_files = false + } 'samtools_index_genome' { args = '' suffix = '.sorted' diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf similarity index 100% rename from modules/nf-core/modules/gatk4/splitncigarreads/functions.nf rename to modules/local/gatk4/splitncigarreads/functions.nf diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/local/gatk4/splitncigarreads/main.nf similarity index 90% rename from modules/nf-core/modules/gatk4/splitncigarreads/main.nf rename to modules/local/gatk4/splitncigarreads/main.nf index 26fb799d..870b4fba 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf +++ b/modules/local/gatk4/splitncigarreads/main.nf @@ -19,7 +19,7 @@ process GATK4_SPLITNCIGARREADS { } input: - tuple val(meta), path(bam) + tuple val(meta), path(bam), path(bai), path(interval) path fasta path fai path dict @@ -30,10 +30,12 @@ process GATK4_SPLITNCIGARREADS { script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def interval_option = interval ? "-L ${interval}" : "" """ gatk SplitNCigarReads \\ -R $fasta \\ -I $bam \\ + ${interval_option} \\ -O ${prefix}.bam \\ $options.args diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml similarity index 100% rename from modules/nf-core/modules/gatk4/splitncigarreads/meta.yml rename to modules/local/gatk4/splitncigarreads/meta.yml diff --git a/subworkflows/local/preprocess.nf b/subworkflows/local/preprocess.nf new file mode 100644 index 00000000..0ae67ece --- /dev/null +++ b/subworkflows/local/preprocess.nf @@ -0,0 +1,56 @@ +// +// PREPROCESSING OF ALIGNMENT FILE OVER THE INTERVALS IN PARALLEL +// + +params.gatk_splitncigar_options = [:] +params.samtools_index_options = [:] +params.samtools_merge_options = [:] + +include { GATK4_SPLITNCIGAR } from '../nf-core/splitn_cigar_reads' addParams(gatk_splitncigar_options: params.gatk_splitncigar_options, samtools_index_options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.samtools_merge_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) + +workflow PREPROCESS { + take: + bam // channel: [ val(meta), [ bam ], [bai] ] + fasta // channel: [ fasta ] + fasta_fai // channel: [ fai ] + fasta_dict // channel: [ dict ] + intervals // channel: [ interval_list] + + main: + + ch_versions = Channel.empty() + + bam.combine(intervals) + .map{ meta, bam, bai, intervals -> + new_meta = meta.clone() + new_meta.id = meta.id + "_" + intervals.baseName + new_meta.sample = meta.id + [new_meta, bam, bai, intervals] + }.set{bam_interval} + + GATK4_SPLITNCIGAR(bam_interval, fasta, fasta_fai, fasta_dict) + bam_splitncigar = GATK4_SPLITNCIGAR.out.bam + ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first()) + + bam_splitncigar + .map{ meta, bam -> + meta.id = meta.sample + [meta, bam] + }.groupTuple().set{bam_splitncigar_interval} + + SAMTOOLS_MERGE(bam_splitncigar_interval, fasta) + bam_splitncigar_merged = SAMTOOLS_MERGE.out.bam + ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) + + SAMTOOLS_INDEX(bam_splitncigar_merged) + bam_splitncigar_merged_index = SAMTOOLS_INDEX.out.bai + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + emit: + + bam = bam_splitncigar_merged + bai = bam_splitncigar_merged_index + versions = ch_versions +} diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf index 3adf6b63..59cba3db 100644 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -6,15 +6,15 @@ params.gatk_splitncigar_options = [:] params.samtools_index_options = [:] params.samtools_stats_options = [:] -include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' addParams( options: params.gatk_splitncigar_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) +include { GATK4_SPLITNCIGARREADS } from '../../modules/local/gatk4/splitncigarreads/main' addParams( options: params.gatk_splitncigar_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) workflow GATK4_SPLITNCIGAR { take: - bam // channel: [ val(meta), [ bam ] ] + bam_interval // channel: [ val(meta), [ bam ], [bai], [interval] ] fasta // channel: [ fasta ] - fasta_fai // channel: [ fai ] - fasta_dict // channel: [ dict ] + fasta_fai // channel: [ fai ] + fasta_dict // channel: [ dict ] main: @@ -23,7 +23,7 @@ workflow GATK4_SPLITNCIGAR { // // GATK4 // - GATK4_SPLITNCIGARREADS ( bam, fasta, fasta_fai, fasta_dict) + GATK4_SPLITNCIGARREADS ( bam_interval, fasta, fasta_fai, fasta_dict) ch_versions = ch_versions.mix(GATK4_SPLITNCIGARREADS.out.versions.first()) // diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 95342adb..76a793f8 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -154,11 +154,13 @@ include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates samtools_index_options: modules['picard_markduplicates_samtools'], samtools_stats_options: modules['picard_markduplicates_samtools'] ) -// Splits reads that contain Ns in their cigar string -include { GATK4_SPLITNCIGAR } from '../subworkflows/nf-core/splitn_cigar_reads' addParams( +// Subworkflow - splits reads that contain Ns in their cigar string +include { PREPROCESS } from '../subworkflows/local/preprocess' addParams( gatk_splitncigar_options: modules['gatk_splitncigar_options'], - samtools_index_options: modules['picard_markduplicates_samtools'] + samtools_index_options: modules['samtools_index_genome'], + samtools_merge_options: modules['samtools_merge_genome'] ) + // Estimate and correct systematic bias include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( applybqsr_options: modules['applybqsr'], @@ -279,8 +281,7 @@ workflow RNAVAR { // SUBWORKFLOW: Mark duplicates with Picard MARKDUPLICATES(ch_genome_bam) - ch_genome_bam = MARKDUPLICATES.out.bam - ch_genome_bam_index = MARKDUPLICATES.out.bai + ch_genome_bam = MARKDUPLICATES.out.bam.join(MARKDUPLICATES.out.bai, by: [0]) ch_samtools_stats = MARKDUPLICATES.out.stats ch_samtools_flagstat = MARKDUPLICATES.out.flagstat ch_samtools_idxstats = MARKDUPLICATES.out.idxstats @@ -288,12 +289,12 @@ workflow RNAVAR { if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi ch_versions = ch_versions.mix(MARKDUPLICATES.out.versions.first().ifEmpty(null)) - // MODULE: SplitNCigarReads from GATK4 + // Subworkflow - SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() - GATK4_SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict) - bam_splitncigar = GATK4_SPLITNCIGAR.out.bam.join(GATK4_SPLITNCIGAR.out.bai, by: [0]) - ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first().ifEmpty(null)) + PREPROCESS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) + bam_splitncigar = PREPROCESS.out.bam.join(PREPROCESS.out.bai, by: [0]) + ch_versions = ch_versions.mix(PREPROCESS.out.versions.first().ifEmpty(null)) // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() @@ -343,7 +344,8 @@ workflow RNAVAR { .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() new_meta.id = meta.id - [new_meta, bam, bai, interval_list]} + [new_meta, bam, bai, interval_list] + } GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, From 79ffd18fb4de1b828ec185e85292d61b293e310c Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 13:08:55 +0100 Subject: [PATCH 066/235] fix: lint errors --- .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/workflows/ci.yml | 5 ++++- .github/workflows/linting_comment.yml | 1 + README.md | 4 ++-- modules.json | 5 +---- nextflow.config | 2 +- 6 files changed, 10 insertions(+), 9 deletions(-) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 200eb840..c1a08712 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -44,7 +44,7 @@ body: attributes: label: System information description: | - * Nextflow version _(eg. 21.04.01)_ + * Nextflow version _(eg. 21.10.3)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index da4a654b..94f75c1d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,7 +23,8 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['21.04.0', ''] + nxf_ver: + - 'NXF_VER=21.10.3' steps: - name: Check out pipeline code uses: actions/checkout@v2 @@ -33,6 +34,8 @@ jobs: run: | wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ + export ${{ matrix.nxf_ver }} + nextflow self-update - name: Run pipeline with test data run: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 90f03c6f..44d72994 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -15,6 +15,7 @@ jobs: uses: dawidd6/action-download-artifact@v2 with: workflow: linting.yml + workflow_conclusion: completed - name: Get PR number id: pr_number diff --git a/README.md b/README.md index a1c0ef07..805822a2 100644 --- a/README.md +++ b/README.md @@ -5,7 +5,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.04.0-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -39,7 +39,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ## Quick Start -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.04.0`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(please only use [`Conda`](https://conda.io/miniconda.html) as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_ diff --git a/modules.json b/modules.json index 8d063c6e..0e780207 100644 --- a/modules.json +++ b/modules.json @@ -36,9 +36,6 @@ "gatk4/mergevcfs": { "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" }, - "gatk4/splitncigarreads": { - "git_sha": "691feeafdcd8b7874f540695ece122cce01a524f" - }, "gatk4/variantfiltration": { "git_sha": "43a1c1c6d18d7d9395846503046f1649ba72a06e" }, @@ -92,4 +89,4 @@ } } } -} \ No newline at end of file +} diff --git a/nextflow.config b/nextflow.config index 33b56492..29af0162 100644 --- a/nextflow.config +++ b/nextflow.config @@ -210,7 +210,7 @@ manifest { homePage = 'https://github.com/nf-core/rnavar' description = 'GATK4 RNA variant calling pipeline' mainScript = 'main.nf' - nextflowVersion = '!>=21.04.0' + nextflowVersion = '!>=21.10.3' version = '1.0dev' } From dcd562efce3b3d72f10895d30f59931de3502eb7 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 13:28:49 +0100 Subject: [PATCH 067/235] fix: lint error in CI --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 94f75c1d..de8ce837 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: matrix: # Nextflow versions: check pipeline minimum and current latest nxf_ver: - - 'NXF_VER=21.10.3' + - '21.10.3' steps: - name: Check out pipeline code uses: actions/checkout@v2 From e349fa6f204f9f132d9f7d249735433d2290cf7a Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 13:31:19 +0100 Subject: [PATCH 068/235] fix: lint error in CI --- .github/workflows/ci.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index de8ce837..d7f856a4 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -18,13 +18,12 @@ jobs: if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/rnavar') }} runs-on: ubuntu-latest env: - NXF_VER: ${{ matrix.nxf_ver }} NXF_ANSI_LOG: false strategy: matrix: # Nextflow versions: check pipeline minimum and current latest nxf_ver: - - '21.10.3' + - 'NXF_VER=21.10.3' steps: - name: Check out pipeline code uses: actions/checkout@v2 From ff4ffc5059dd115a21e1e5724b6b93f0a4c8a270 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 15:29:26 +0100 Subject: [PATCH 069/235] update: splitNCigar runs in parallel --- nextflow_schema.json | 6 +++--- subworkflows/local/{preprocess.nf => splitncigar.nf} | 2 +- workflows/rnavar.nf | 8 ++++---- 3 files changed, 8 insertions(+), 8 deletions(-) rename subworkflows/local/{preprocess.nf => splitncigar.nf} (98%) diff --git a/nextflow_schema.json b/nextflow_schema.json index a046bacf..9d514f0e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -312,17 +312,17 @@ "description": "Define parameters that control the stages in the pipeline", "properties": { "skip_intervallisttools": { - "type": "string", + "type": "boolean", "description": "Skip the process of preparing interval lists for the GATK variant calling step", "help_text": "This parameter disable preparing multiple interval lists to use with HaplotypeCaller module of GATK. It is recommended not to disable the step as it is required to run the variant calling correctly." }, "skip_variantfiltration": { - "type": "string", + "type": "boolean", "description": "Skip variant filtering of GATK", "help_text": "Set this parameter if you don't want to filter any variants." }, "skip_variantannotation": { - "type": "string", + "type": "boolean", "description": "Skip variant annotation", "help_text": "Set this parameter if you don't want to run variant annotation." }, diff --git a/subworkflows/local/preprocess.nf b/subworkflows/local/splitncigar.nf similarity index 98% rename from subworkflows/local/preprocess.nf rename to subworkflows/local/splitncigar.nf index 0ae67ece..6f0f480d 100644 --- a/subworkflows/local/preprocess.nf +++ b/subworkflows/local/splitncigar.nf @@ -10,7 +10,7 @@ include { GATK4_SPLITNCIGAR } from '../nf-core/splitn_cigar_reads' include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.samtools_merge_options) include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) -workflow PREPROCESS { +workflow SPLITNCIGAR { take: bam // channel: [ val(meta), [ bam ], [bai] ] fasta // channel: [ fasta ] diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 76a793f8..b487c13c 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -155,7 +155,7 @@ include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates samtools_stats_options: modules['picard_markduplicates_samtools'] ) // Subworkflow - splits reads that contain Ns in their cigar string -include { PREPROCESS } from '../subworkflows/local/preprocess' addParams( +include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' addParams( gatk_splitncigar_options: modules['gatk_splitncigar_options'], samtools_index_options: modules['samtools_index_genome'], samtools_merge_options: modules['samtools_merge_genome'] @@ -292,9 +292,9 @@ workflow RNAVAR { // Subworkflow - SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() - PREPROCESS(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) - bam_splitncigar = PREPROCESS.out.bam.join(PREPROCESS.out.bai, by: [0]) - ch_versions = ch_versions.mix(PREPROCESS.out.versions.first().ifEmpty(null)) + SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) + bam_splitncigar = GATK4_SPLITNCIGAR.out.bam.join(GATK4_SPLITNCIGAR.out.bai, by: [0]) + ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first().ifEmpty(null)) // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() From 042251a82149c5c381b7b824b3a6021480f21ccf Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 15:32:23 +0100 Subject: [PATCH 070/235] update: splitNCigar runs in parallel --- workflows/rnavar.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index b487c13c..ec0b9e83 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -293,8 +293,8 @@ workflow RNAVAR { // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). bam_splitncigar = Channel.empty() SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) - bam_splitncigar = GATK4_SPLITNCIGAR.out.bam.join(GATK4_SPLITNCIGAR.out.bai, by: [0]) - ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first().ifEmpty(null)) + bam_splitncigar = SPLITNCIGAR.out.bam.join(SPLITNCIGAR.out.bai, by: [0]) + ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() From 63e29bf1bc24375d8376af5a77094c8b782fc3ca Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 30 Nov 2021 15:39:04 +0100 Subject: [PATCH 071/235] update: splitNCigar runs in parallel --- docs/usage.md | 10 +--------- 1 file changed, 1 insertion(+), 9 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 35aaaafc..e6b2029c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -53,7 +53,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p ## Alignment options -The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome, project the alignments onto the transcriptome and to perform the downstream BAM-level quantification with [Salmon](https://salmon.readthedocs.io/en/latest/salmon.html). STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. +The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome. STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. ## Reference genome files @@ -62,17 +62,9 @@ The minimum reference genome requirements are a FASTA and GTF file, all other fi * If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. * If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. * If `--gene_bed` is not provided then it will be generated from the GTF file. -* If `--additional_fasta` is provided then the features in this file (e.g. ERCC spike-ins) will be automatically concatenated onto both the reference FASTA file as well as the GTF annotation before building the appropriate indices. - -When using `--aligner star_rsem`, both the STAR and RSEM indices should be present in the path specified by `--rsem_index` (see [#568](https://github.com/nf-core/rnavar/issues/568)) > **NB:** Compressed reference files are also supported by the pipeline i.e. standard files with the `.gz` extension and indices folders with the `tar.gz` extension. -If you are using [GENCODE](https://www.gencodegenes.org/) reference genome files please specify the `--gencode` parameter because the format of these files is slightly different to ENSEMBL genome files: - -* The `--gtf_group_features_type` parameter will automatically be set to `gene_type` as opposed to `gene_biotype`, respectively. -* If you are running Salmon, the `--gencode` flag will also be passed to the index building step to overcome parsing issues resulting from the transcript IDs in GENCODE fasta files being separated by vertical pipes (`|`) instead of spaces (see [this issue](https://github.com/COMBINE-lab/salmon/issues/15)). - ## Running the pipeline The typical command for running the pipeline is as follows: From b8cb3ee0eabc24744c6a9398dfe02423fe7e3b4a Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 30 Nov 2021 22:20:52 +0100 Subject: [PATCH 072/235] fix: cache variable --- modules/nf-core/modules/snpeff/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/modules/snpeff/main.nf b/modules/nf-core/modules/snpeff/main.nf index 9652f110..77a36de7 100644 --- a/modules/nf-core/modules/snpeff/main.nf +++ b/modules/nf-core/modules/snpeff/main.nf @@ -41,7 +41,7 @@ process SNPEFF { avail_mem = task.memory.giga } def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - cache = params.use_cache ? "-dataDir \${PWD}/${snpeff_cache}" : "" + cache = params.use_cache ? "-dataDir \${PWD}/${cache}" : "" """ snpEff -Xmx${avail_mem}g \\ $db \\ From 2a0617eae31dd048ea9eba351defced4ff14c0bc Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 30 Nov 2021 22:28:42 +0100 Subject: [PATCH 073/235] fix: cache variable correction --- modules/nf-core/modules/snpeff/main.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/nf-core/modules/snpeff/main.nf b/modules/nf-core/modules/snpeff/main.nf index 77a36de7..7e8f9d73 100644 --- a/modules/nf-core/modules/snpeff/main.nf +++ b/modules/nf-core/modules/snpeff/main.nf @@ -40,14 +40,14 @@ process SNPEFF { } else { avail_mem = task.memory.giga } - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - cache = params.use_cache ? "-dataDir \${PWD}/${cache}" : "" + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def dir_cache = params.use_cache ? "-dataDir \${PWD}/${cache}" : "" """ snpEff -Xmx${avail_mem}g \\ $db \\ $options.args \\ -csvStats ${prefix}.csv \\ - $cache \\ + $dir_cache \\ $vcf \\ > ${prefix}.ann.vcf From ca3c434945338d71d1e2df55c39652603f476da8 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 7 Dec 2021 12:23:09 +0100 Subject: [PATCH 074/235] Fix: custom star index version check; --vcf syntax update for VEP --- conf/modules.config | 6 ++--- modules/nf-core/modules/ensemblvep/main.nf | 2 +- nextflow.config | 2 +- nextflow_schema.json | 2 +- subworkflows/local/prepare_genome.nf | 27 +++++++++++++++++++--- 5 files changed, 30 insertions(+), 9 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index ed4a34af..38a45d12 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -138,7 +138,7 @@ params { } 'bgziptabix_snpeff' { publish_dir = 'variant_annotation' - suffix = '_snpEff.ann' + suffix = '_snpEff.ann.vcf' publish_by_meta = true } 'merge_vep' { @@ -147,7 +147,7 @@ params { } 'bgziptabix_merge_vep' { publish_dir = 'variant_annotation' - suffix = '_snpEff_VEP.ann' + suffix = '_snpEff_VEP.ann.vcf' publish_by_meta = true } 'ensemblvep' { @@ -157,7 +157,7 @@ params { } 'bgziptabix_vep' { publish_dir = 'variant_annotation' - suffix = '_VEP.ann' + suffix = '_VEP.ann.vcf' publish_by_meta = true } // QC modules diff --git a/modules/nf-core/modules/ensemblvep/main.nf b/modules/nf-core/modules/ensemblvep/main.nf index ad9c38a6..416e58ef 100644 --- a/modules/nf-core/modules/ensemblvep/main.nf +++ b/modules/nf-core/modules/ensemblvep/main.nf @@ -51,7 +51,7 @@ process ENSEMBLVEP { --cache_version $cache_version \\ --dir_cache $dir_cache \\ --fork $task.cpus \\ - --format vcf \\ + --vcf \\ --stats_file ${prefix}.summary.html rm -rf $prefix diff --git a/nextflow.config b/nextflow.config index 29af0162..a0049926 100644 --- a/nextflow.config +++ b/nextflow.config @@ -58,7 +58,7 @@ params { skip_variantannotation = false // GATK intervallist parameters - scatter_count = 6 + scatter_count = 50 //GATK variant filter parameters window = 35 diff --git a/nextflow_schema.json b/nextflow_schema.json index 9d514f0e..b02bff0f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -341,7 +341,7 @@ "properties": { "scatter_count": { "type": "integer", - "default": 6, + "default": 50, "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", "help_text": "Set this parameter to decide the number of splits for the gene interval list file." }, diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 2f0aea2f..69e04db1 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -96,6 +96,8 @@ workflow PREPARE_GENOME { // ch_star_index = Channel.empty() ch_star_version = Channel.empty() + run_star_index = false + if ('star' in prepare_tool_indices) { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { @@ -104,10 +106,29 @@ workflow PREPARE_GENOME { } else { ch_star_index = file(params.star_index) } - } else { - ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index - ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + + // Check version of STAR index for compatibility with pipeline STAR version 2.7.9a + allowed_star_versions = ["2.7.4a"] //STAR version 2.7.9a writes 2.7.4a in the genomeParameters file. + star_genome_file = file("${ch_star_index}/genomeParameters.txt", checkIfExits: true) + for (line: star_genome_file.readLines()){ + if(line.startsWith("versionGenome")){ + fields = line.split("\t") + custom_star_version = fields[1].trim() + if (!(custom_star_version in allowed_star_versions)){ + run_star_index = true + } + break + } + } } + else { + run_star_index = true + } + } + + if (run_star_index){ + ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) } emit: From bbb86b62043d486f82ce1a909d159863509f44ec Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 7 Dec 2021 12:43:09 +0100 Subject: [PATCH 075/235] Added GATK parameters --- conf/modules.config | 6 +++--- nextflow.config | 5 ++++- nextflow_schema.json | 19 ++++++++++++++++++- workflows/rnavar.nf | 3 +++ 4 files changed, 28 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 38a45d12..74f79afe 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -66,17 +66,17 @@ params { publish_files = false } 'gatk_baserecalibrator' { - args = '' + args = '--use-original-qualities' suffix = '' publish_files = false } 'gatk_applybqsr' { - args = '' + args = '--use-original-qualities --add-output-sam-program-record' suffix = '.markdup.recalibrated' publish_files = false } 'gatk_haplotypecaller' { - args = '-dont-use-soft-clipped-bases --standard-min-confidence-threshold-for-calling 20' + args = '-dont-use-soft-clipped-bases' suffix = '' publish_files = false } diff --git a/nextflow.config b/nextflow.config index a0049926..0064a035 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,7 +22,7 @@ params { save_merged_fastq = false // Sequence read information - read_length = 150 // Required for STAR to build index and align reads + read_length = 151 // Required for STAR to build index and align reads // Alignment aligner = 'star' // Only STAR is currently supported. @@ -60,6 +60,9 @@ params { // GATK intervallist parameters scatter_count = 50 + //GATK haplotypecaller parameters + stand_call_conf = 20 + //GATK variant filter parameters window = 35 cluster = 3 diff --git a/nextflow_schema.json b/nextflow_schema.json index b02bff0f..e01aeca3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -112,7 +112,7 @@ }, "read_length": { "type": "number", - "default": 150, + "default": 151, "description": "Read length", "help_text": "Specify the read length for the STAR aligner." }, @@ -231,6 +231,23 @@ } } }, + "variant_calling": { + "title": "Variant calling", + "type": "object", + "description": "", + "default": "", + "fa_icon": "fas fa-toolbox", + "properties": { + "stand_call_conf": { + "type": "number", + "default": 20, + "fa_icon": "fas fa-hammer", + "description": "The minimum phred-scaled confidence threshold at which variants should be called.", + "help_text": "Specify the minimum phred-scaled confidence threshold at which variants should be called.", + "hidden": false + } + } + }, "variant_annotation": { "title": "Variant Annotation", "type": "object", diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index ec0b9e83..1733095b 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -97,6 +97,7 @@ if (params.save_align_intermeds) { if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false +// Additional paramaters to modules based on params modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' modules['star_align'].args += params.star_twopass ? Utils.joinModuleArgs(['--twopassMode Basic']) : '' if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') @@ -106,6 +107,8 @@ modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.j modules['picard_markduplicates'].args += params.remove_duplicates ? Utils.joinModuleArgs(['REMOVE_DUPLICATES=true']) : '' modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) +modules['gatk_haplotypecaller'].args += Utils.joinModuleArgs(["--standard-min-confidence-threshold-for-calling $params.stand_call_conf"]) + if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) From 5a7d954828f2987cbb1c15032f4d6747bc0b3426 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 7 Dec 2021 12:50:49 +0100 Subject: [PATCH 076/235] Fix: lint error --- nextflow_schema.json | 3 +++ 1 file changed, 3 insertions(+) diff --git a/nextflow_schema.json b/nextflow_schema.json index e01aeca3..99636b21 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -602,6 +602,9 @@ { "$ref": "#/definitions/alignment_options" }, + { + "$ref": "#/definitions/variant_calling" + }, { "$ref": "#/definitions/variant_annotation" }, From 1bd2b26e3b9d1e3c02eeba463d132315cc38c395 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 8 Dec 2021 12:49:06 +0100 Subject: [PATCH 077/235] for expert review --- nextflow.config | 2 +- subworkflows/local/prepare_genome.nf | 29 +++++++++++++++++----------- 2 files changed, 19 insertions(+), 12 deletions(-) diff --git a/nextflow.config b/nextflow.config index 0064a035..f40e64c4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,7 +22,7 @@ params { save_merged_fastq = false // Sequence read information - read_length = 151 // Required for STAR to build index and align reads + read_length = 150 // Required for STAR to build index and align reads // Alignment aligner = 'star' // Only STAR is currently supported. diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 69e04db1..163d8a93 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -109,17 +109,12 @@ workflow PREPARE_GENOME { // Check version of STAR index for compatibility with pipeline STAR version 2.7.9a allowed_star_versions = ["2.7.4a"] //STAR version 2.7.9a writes 2.7.4a in the genomeParameters file. - star_genome_file = file("${ch_star_index}/genomeParameters.txt", checkIfExits: true) - for (line: star_genome_file.readLines()){ - if(line.startsWith("versionGenome")){ - fields = line.split("\t") - custom_star_version = fields[1].trim() - if (!(custom_star_version in allowed_star_versions)){ - run_star_index = true - } - break - } - } + ch_star_index.map{ dir -> getIndexVersion(dir) }.view() + + //custom_star_version = getVersion(ch_star_index) + //if (!(custom_star_version in allowed_star_versions)){ + // run_star_index = true + //} } else { run_star_index = true @@ -131,6 +126,7 @@ workflow PREPARE_GENOME { ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) } + emit: fasta = ch_fasta // path: genome.fasta fai = ch_fasta_fai // path: genome.fasta.fai @@ -141,3 +137,14 @@ workflow PREPARE_GENOME { star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } + +def getIndexVersion( index_path ) { + genomeParameters = new File("$index_path/genomeParameters.txt") + if ( genomeParameters.exists() ) { + genomeParameters.text.eachLine() { line -> + if(line.startsWith("versionGenome")){ + return line.split("\t")[1].trim() + } + } + } +} From 82b10dafb994a15ef1cc9f430188f4db01f6efce Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 9 Dec 2021 16:58:45 +0100 Subject: [PATCH 078/235] check version of custom star_index --- subworkflows/local/prepare_genome.nf | 40 +++++++++++++++------------- 1 file changed, 22 insertions(+), 18 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 163d8a93..5ea5c95a 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -33,7 +33,7 @@ workflow PREPARE_GENOME { // Uncompress genome fasta file if required // if (params.fasta.endsWith('.gz')) { - ch_fasta = GUNZIP_FASTA ( params.fasta ).gunzip + ch_fasta = (GUNZIP_FASTA ( params.fasta ).gunzip) ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { ch_fasta = file(params.fasta) @@ -42,6 +42,7 @@ workflow PREPARE_GENOME { // // Uncompress GTF annotation file or create from GFF3 if required // + ch_gffread_version = Channel.empty() if (params.gtf) { if (params.gtf.endsWith('.gz')) { @@ -94,9 +95,8 @@ workflow PREPARE_GENOME { // // Uncompress STAR index or generate from scratch if required // - ch_star_index = Channel.empty() - ch_star_version = Channel.empty() - run_star_index = false + ch_star_index = Channel.empty() + ch_fasta_for_index = Channel.empty() if ('star' in prepare_tool_indices) { if (params.star_index) { @@ -104,28 +104,32 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = file(params.star_index) + ch_star_index = Channel.fromPath(params.star_index) } - // Check version of STAR index for compatibility with pipeline STAR version 2.7.9a - allowed_star_versions = ["2.7.4a"] //STAR version 2.7.9a writes 2.7.4a in the genomeParameters file. - ch_star_index.map{ dir -> getIndexVersion(dir) }.view() + // Get the version of the index and branch into valid or invalid + ch_star_index.branch { dir -> + valid_index: getIndexVersion(dir) == '2.7.4a' + return ch_star_index + invalid_index: getIndexVersion(dir) != '2.7.4a' + return ch_fasta + }.set{result} - //custom_star_version = getVersion(ch_star_index) - //if (!(custom_star_version in allowed_star_versions)){ - // run_star_index = true - //} + ch_fasta_for_index = result.invalid_index } else { - run_star_index = true + ch_fasta_for_index = ch_fasta } - } - if (run_star_index){ - ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index + //ch_star_index.view() + //ch_fasta_for_index.view() + + ch_star_index = STAR_GENOMEGENERATE (ch_fasta_for_index,ch_gtf).index ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) - } + //ch_star_index.view() + + } emit: fasta = ch_fasta // path: genome.fasta @@ -141,7 +145,7 @@ workflow PREPARE_GENOME { def getIndexVersion( index_path ) { genomeParameters = new File("$index_path/genomeParameters.txt") if ( genomeParameters.exists() ) { - genomeParameters.text.eachLine() { line -> + for(line: genomeParameters.readLines()){ if(line.startsWith("versionGenome")){ return line.split("\t")[1].trim() } From e94e65d4df8fc7bfdbafe96386532581188db6e3 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 9 Dec 2021 18:33:48 +0100 Subject: [PATCH 079/235] withdraw version check of custom star_index --- subworkflows/local/prepare_genome.nf | 39 +++------------------------- 1 file changed, 4 insertions(+), 35 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 5ea5c95a..54df2cb3 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -96,7 +96,6 @@ workflow PREPARE_GENOME { // Uncompress STAR index or generate from scratch if required // ch_star_index = Channel.empty() - ch_fasta_for_index = Channel.empty() if ('star' in prepare_tool_indices) { if (params.star_index) { @@ -104,31 +103,12 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = Channel.fromPath(params.star_index) + ch_star_index = file(params.star_index) } - - // Get the version of the index and branch into valid or invalid - ch_star_index.branch { dir -> - valid_index: getIndexVersion(dir) == '2.7.4a' - return ch_star_index - invalid_index: getIndexVersion(dir) != '2.7.4a' - return ch_fasta - }.set{result} - - ch_fasta_for_index = result.invalid_index - } - else { - ch_fasta_for_index = ch_fasta + } else { + ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) } - - //ch_star_index.view() - //ch_fasta_for_index.view() - - ch_star_index = STAR_GENOMEGENERATE (ch_fasta_for_index,ch_gtf).index - ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) - - //ch_star_index.view() - } emit: @@ -141,14 +121,3 @@ workflow PREPARE_GENOME { star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } - -def getIndexVersion( index_path ) { - genomeParameters = new File("$index_path/genomeParameters.txt") - if ( genomeParameters.exists() ) { - for(line: genomeParameters.readLines()){ - if(line.startsWith("versionGenome")){ - return line.split("\t")[1].trim() - } - } - } -} From ce1f437c986ebb13521bc6121a307b729684ff18 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 9 Dec 2021 19:36:08 +0100 Subject: [PATCH 080/235] Minor fixes --- nextflow_schema.json | 3 ++- subworkflows/local/prepare_genome.nf | 36 ++++++++++++++++++++++++---- 2 files changed, 34 insertions(+), 5 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 99636b21..8f7e7928 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -43,7 +43,8 @@ "fa_icon": "fas fa-file-signature" }, "save_merged_fastq": { - "type": "string", + "type": "boolean", + "default": false, "description": "Save FastQ files after merging re-sequenced libraries in the results directory." } } diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 54df2cb3..77b0e4b2 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -96,6 +96,7 @@ workflow PREPARE_GENOME { // Uncompress STAR index or generate from scratch if required // ch_star_index = Channel.empty() + ch_fasta_for_index = Channel.empty() if ('star' in prepare_tool_indices) { if (params.star_index) { @@ -103,12 +104,28 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = file(params.star_index) + ch_star_index = Channel.fromPath(params.star_index) } - } else { - ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index - ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + + // Get the version of the index and branch into valid or invalid + ch_star_index.branch { dir -> + valid_index: getIndexVersion(dir) == '2.7.4a' + return dir + invalid_index: getIndexVersion(dir) != '2.7.4a' + return ch_fasta + }.set{result} + + ch_star_index = result.valid_index + ch_fasta_for_index = result.invalid_index } + else { + ch_fasta_for_index = ch_fasta + } + + ch_star_index_optional = STAR_GENOMEGENERATE (ch_fasta_for_index,ch_gtf).index + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + + ch_star_index = ch_star_index.mix(ch_star_index_optional) } emit: @@ -121,3 +138,14 @@ workflow PREPARE_GENOME { star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } + +def getIndexVersion( index_path ) { + genomeParameters = new File("$index_path/genomeParameters.txt") + if ( genomeParameters.exists() ) { + for(line: genomeParameters.readLines()){ + if(line.startsWith("versionGenome")){ + return line.split("\t")[1].trim() + } + } + } +} From 7691cef193da6261209b313afc1bfdf7f6231268 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 9 Dec 2021 20:56:51 +0100 Subject: [PATCH 081/235] Fix: Test errors --- .github/workflows/ci.yml | 36 ++++++++++++++++++++-------- assets/sendmail_template.txt | 4 ++-- conf/modules.config | 8 +++++-- docs/images/nf-core-rnavar_logo.png | Bin 32098 -> 0 bytes nextflow.config | 8 +++---- nextflow_schema.json | 15 ++++++++---- workflows/rnavar.nf | 7 +++--- 7 files changed, 52 insertions(+), 26 deletions(-) delete mode 100644 docs/images/nf-core-rnavar_logo.png diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index d7f856a4..bd779e38 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -8,9 +8,6 @@ on: release: types: [published] -# Uncomment if we need an edge release of Nextflow again -# env: NXF_EDGE: 1 - jobs: test: name: Run workflow tests @@ -21,21 +18,40 @@ jobs: NXF_ANSI_LOG: false strategy: matrix: - # Nextflow versions: check pipeline minimum and current latest - nxf_ver: - - 'NXF_VER=21.10.3' + # Nextflow versions + include: + # Test pipeline minimum Nextflow version + - NXF_VER: '21.10.3' + NXF_EDGE: '' + # Test latest edge release of Nextflow + - NXF_VER: '' + NXF_EDGE: '1' + test: + - 'aligner' + - 'annotation' + - 'default' + # - 'variant_calling' steps: - name: Check out pipeline code uses: actions/checkout@v2 + - name: Install Nextflow env: CAPSULE_LOG: none + NXF_VER: ${{ matrix.NXF_VER }} + # Uncomment only if the edge release is more recent than the latest stable release + # See https://github.com/nextflow-io/nextflow/issues/2467 + # NXF_EDGE: ${{ matrix.NXF_EDGE }} run: | wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ - export ${{ matrix.nxf_ver }} nextflow self-update + - name: Set up Python + uses: actions/setup-python@v2 + with: + python-version: '3.x' + - name: Install dependencies + run: python -m pip install --upgrade pip pytest-workflow - - name: Run pipeline with test data - run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker + - name: Run pipeline with tests settings + run: pytest --tag ${{ matrix.test }} --kwdof diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index a1f8503c..e10ee80e 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -12,9 +12,9 @@ $email_html Content-Type: image/png;name="nf-core-rnavar_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="nf-core-rnavar_logo.png" +Content-Disposition: inline; filename="nf-core-rnavar_logo_light.png" -<% out << new File("$projectDir/assets/nf-core-rnavar_logo.png"). +<% out << new File("$projectDir/assets/nf-core-rnavar_logo_light.png"). bytes. encodeBase64(). toString(). diff --git a/conf/modules.config b/conf/modules.config index 74f79afe..469f5713 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -31,11 +31,15 @@ params { } 'star_align' { args = "--outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" - publish_files = false + publish_dir = "preprocessing" + publish_files = [:] + publish_by_meta = true } 'samtools_sort_genome' { suffix = '.sorted' - publish_files = false + publish_files = [:] + publish_dir = "preprocessing" + publish_by_meta = true } 'samtools_merge_genome' { suffix = '.merged' diff --git 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cadd_cache = null // CADD cache disabled - cadd_indels = false // No CADD InDels file - cadd_indels_tbi = false // No CADD InDels index - cadd_wg_snvs = false // No CADD SNVs file - cadd_wg_snvs_tbi = false // No CADD SNVs index + cadd_indels = null // No CADD InDels file + cadd_indels_tbi = null // No CADD InDels index + cadd_wg_snvs = null // No CADD SNVs file + cadd_wg_snvs_tbi = null // No CADD SNVs index genesplicer = null // genesplicer disabled within VEP snpeff_cache = null // No directory for snpEff cache vep_cache = null // No directory for VEP cache diff --git a/nextflow_schema.json b/nextflow_schema.json index 8f7e7928..e73e0d06 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -118,7 +118,8 @@ "help_text": "Specify the read length for the STAR aligner." }, "save_reference": { - "type": "string", + "type": "boolean", + "default": false, "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", "description": "If generated by the pipeline, save the STAR index in the results directory." }, @@ -185,7 +186,8 @@ "help_text": "This parameter enables STAR to perform 2-pass mapping. Default true." }, "star_ignore_sjdbgtf": { - "type": "string", + "type": "boolean", + "default": false, "description": "Do not use GTF file during STAR index buidling step", "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." }, @@ -201,12 +203,14 @@ "help_text": "This parameter is required for creating a proper BAM header to use in the downstream analysis of GATK. " }, "save_unaligned": { - "type": "string", + "type": "boolean", + "default": false, "description": "Where possible, save unaligned reads from aligner to the results directory.", "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, "save_align_intermeds": { - "type": "string", + "type": "boolean", + "default": false, "description": "Save the intermediate BAM files from the alignment step.", "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." }, @@ -345,7 +349,8 @@ "help_text": "Set this parameter if you don't want to run variant annotation." }, "skip_qc": { - "type": "string", + "type": "boolean", + "default": false, "description": "Skip QC reports", "help_text": "This parameter disable all QC reports from recalibrated BAM file." } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 1733095b..fe5eb00a 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -84,15 +84,16 @@ prepareToolIndices = params.aligner def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] def untar_options = [publish_files: false] +def samtools_sort_genome_options = modules['samtools_sort_genome'] if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' if (params.save_align_intermeds) { - modules['samtools_sort_genome'].publish_files.put('bam','') - modules['samtools_index_genome'].publish_files.put('bai','') - modules['samtools_index_genome'].publish_files.put('csi','') + samtools_sort_genome_options.publish_files.put('bam','') + samtools_sort_genome_options.publish_files.put('bai','') + samtools_sort_genome_options.publish_files.put('csi','') } if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false From 00ed2ad1954601ba840025dca35f5133ce68b557 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 9 Dec 2021 21:40:00 +0100 Subject: [PATCH 082/235] Fix: Test errors - attempt 2 --- assets/nf-core-rnavar_logo_light.png | Bin 0 -> 65097 bytes docs/images/nf-core-rnavar_logo_dark.png | Bin 0 -> 65175 bytes docs/images/nf-core-rnavar_logo_light.png | Bin 0 -> 65097 bytes 3 files changed, 0 insertions(+), 0 deletions(-) create mode 100644 assets/nf-core-rnavar_logo_light.png create mode 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actions/checkout@v2 @@ -54,4 +49,4 @@ jobs: run: python -m pip install --upgrade pip pytest-workflow - name: Run pipeline with tests settings - run: pytest --tag ${{ matrix.test }} --kwdof + run: nextflow run {GITHUB_WORKSPACE} -profile test,docker diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index c5c9fe38..3c9ccf17 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -120,7 +120,7 @@ jobs: - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core git+https://github.com/nf-core/tools.git@dev + pip install --upgrade --force-reinstall git+https://github.com/nf-core/tools.git@dev - name: Run nf-core lint env: diff --git a/assets/nf-core-rnavar_logo.png b/assets/nf-core-rnavar_logo.png deleted file mode 100644 index 482af6ee764c26783cee9e3e82ab3a7c5a892415..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 16469 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Dec 2021 22:55:01 +0100 Subject: [PATCH 084/235] Fix: lint errors - attempt 4 --- .nf-core.yml | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index fcc940f7..4ac5d68f 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,10 @@ lint: files_unchanged: - .github/workflows/linting.yml + - .github/CONTRIBUTING.md - assets/multiqc_config.yaml - - assets/nf-core-rnavar_logo.png - - docs/images/nf-core-rnavar_logo.png + - assets/nf-core-rnavar_logo_light.png + - docs/images/nf-core-rnavar_logo_light.png + - docs/images/nf-core-rnavar_logo_dark.png + - lib/NfcoreTemplate.groovy + - lib/NfcoreSchema.groovy From 17147b7e47e820a84c2c17d80b876ae2dd1b8d5c Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 10 Dec 2021 01:01:08 +0100 Subject: [PATCH 085/235] Fix: CI tests --- .github/workflows/ci.yml | 6 +++- conf/test.config | 2 +- modules/nf-core/modules/ensemblvep/main.nf | 2 +- nextflow.config | 2 +- tests/config/nextflow.config | 35 ++++++++++++++++++++++ tests/config/pytest_software.yml | 4 +++ tests/config/test_data.config | 15 ++++++++++ tests/csv/samplesheet_test.csv | 2 ++ tests/test_annotation.yml | 28 +++++++++++++++++ tests/test_default.yml | 13 ++++++++ tests/test_removeduplicates.yml | 13 ++++++++ 11 files changed, 118 insertions(+), 4 deletions(-) create mode 100644 tests/config/nextflow.config create mode 100644 tests/config/pytest_software.yml create mode 100644 tests/config/test_data.config create mode 100644 tests/csv/samplesheet_test.csv create mode 100644 tests/test_annotation.yml create mode 100644 tests/test_default.yml create mode 100644 tests/test_removeduplicates.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index bc7bb3fb..63d19123 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -26,6 +26,10 @@ jobs: # Test latest edge release of Nextflow - NXF_VER: '' NXF_EDGE: '1' + test: + - 'default' + - 'annotation' + - 'removeduplicates' steps: - name: Check out pipeline code uses: actions/checkout@v2 @@ -49,4 +53,4 @@ jobs: run: python -m pip install --upgrade pip pytest-workflow - name: Run pipeline with tests settings - run: nextflow run {GITHUB_WORKSPACE} -profile test,docker + run: pytest --tag ${{matrix.test}} --kwdof diff --git a/conf/test.config b/conf/test.config index f9ca0800..15dcf743 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,7 @@ params { max_time = 6.h // Input data - input = 'assets/samplesheet_test.csv' + input = "${baseDir}/tests/csv/samplesheet_test.csv" // Genome references genome = 'WBcel235' diff --git a/modules/nf-core/modules/ensemblvep/main.nf b/modules/nf-core/modules/ensemblvep/main.nf index 416e58ef..282dcddd 100644 --- a/modules/nf-core/modules/ensemblvep/main.nf +++ b/modules/nf-core/modules/ensemblvep/main.nf @@ -24,7 +24,7 @@ process ENSEMBLVEP { } input: - tuple val(meta), path(vcf) + tuple val(meta), path(vcf), path(tbi) val genome val species val cache_version diff --git a/nextflow.config b/nextflow.config index 6631de43..d269b8d0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -41,7 +41,7 @@ params { no_intervals = false // Variant annotation - annotate_tools = 'merge' // List of annotation tools to run - snpeff or vep or merge + annotate_tools = null // List of annotation tools to run - snpeff or vep or merge annotation_cache = false // Annotation cache disabled cadd_cache = null // CADD cache disabled cadd_indels = null // No CADD InDels file diff --git a/tests/config/nextflow.config b/tests/config/nextflow.config new file mode 100644 index 00000000..74e1c2d5 --- /dev/null +++ b/tests/config/nextflow.config @@ -0,0 +1,35 @@ +params { + outdir = "output/" + publish_dir_mode = "copy" + enable_conda = false + singularity_pull_docker_container = false + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h +} + +process { + cpus = 2 + memory = 6.GB + time = 48.h +} + +if ("$PROFILE" == "singularity") { + singularity.enabled = true + singularity.autoMounts = true +} else if ("$PROFILE" == "conda") { + params.enable_conda = true +} else { + docker.enabled = true + docker.runOptions = '-u \$(id -u):\$(id -g)' +} + +// Load test_data.config containing paths to test data +includeConfig 'test_data.config' + +// Load modules.config for default module params +includeConfig '../../conf/modules.config' + +manifest { + nextflowVersion = '!>=21.10.3' +} diff --git a/tests/config/pytest_software.yml b/tests/config/pytest_software.yml new file mode 100644 index 00000000..b215cffc --- /dev/null +++ b/tests/config/pytest_software.yml @@ -0,0 +1,4 @@ +markduplicates: + - modules/nf-core/software/gatk4/markduplicates/main.nf + - subworkflow/local/markduplicates.nf + - tests/subworkflow/local/markduplicates/** diff --git a/tests/config/test_data.config b/tests/config/test_data.config new file mode 100644 index 00000000..665947a5 --- /dev/null +++ b/tests/config/test_data.config @@ -0,0 +1,15 @@ +def test_data_dir = "${launchDir}/tests/data/" +def nf_core_modules_data = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/" + +try { + includeConfig "https://raw.githubusercontent.com/nf-core/modules/master/tests/config/test_data.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/modules test data config") +} + +params { + test_data { + 'external' { + } + } +} diff --git a/tests/csv/samplesheet_test.csv b/tests/csv/samplesheet_test.csv new file mode 100644 index 00000000..7131bd5d --- /dev/null +++ b/tests/csv/samplesheet_test.csv @@ -0,0 +1,2 @@ +sample,fastq_1,fastq_2,strandedness +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/tests/test_annotation.yml b/tests/test_annotation.yml new file mode 100644 index 00000000..1ca2cbc0 --- /dev/null +++ b/tests/test_annotation.yml @@ -0,0 +1,28 @@ +- name: Run snpEff + command: nextflow run main.nf -profile test,docker --annotate_tools snpeff + tags: + - annotation + - snpeff + files: + - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz + - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi +- name: Run VEP + command: nextflow run main.nf -profile test,docker --annotate_tools vep + tags: + - annotation + - vep + files: + - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz + - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz.tbi +- name: Run snpEff followed by VEP + command: nextflow run main.nf -profile test,docker --annotate_tools merge + tags: + - annotation + - merge + - snpeff + - vep + files: + - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz + - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi + - - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz + - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz.tbi diff --git a/tests/test_default.yml b/tests/test_default.yml new file mode 100644 index 00000000..508d5aa3 --- /dev/null +++ b/tests/test_default.yml @@ -0,0 +1,13 @@ +- name: Run default pipeline + command: nextflow run main.nf -profile test,docker + tags: + - default + - preprocessing + files: + - path: results/reports/multiqc_report.html + - path: results/preprocessing/GM12878/GM12878.recal.merge.bam + - path: results/preprocessing/GM12878/GM12878.recal.merge.bam.bai + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi + - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz + - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz.tbi diff --git a/tests/test_removeduplicates.yml b/tests/test_removeduplicates.yml new file mode 100644 index 00000000..164bee21 --- /dev/null +++ b/tests/test_removeduplicates.yml @@ -0,0 +1,13 @@ +- name: Run default pipeline + command: nextflow run main.nf -profile test,docker --remove_duplicates true + tags: + - default + - preprocessing + files: + - path: results/reports/multiqc_report.html + - path: results/preprocessing/GM12878/GM12878.recal.merge.bam + - path: results/preprocessing/GM12878/GM12878.recal.merge.bam.bai + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi + - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz + - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz.tbi From 46e605fc3e86954ccc78a5f4df37289157147dbd Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 10 Dec 2021 01:35:07 +0100 Subject: [PATCH 086/235] Fix: CI error in test_annotation.yml --- tests/test_annotation.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_annotation.yml b/tests/test_annotation.yml index 1ca2cbc0..5ccd131b 100644 --- a/tests/test_annotation.yml +++ b/tests/test_annotation.yml @@ -24,5 +24,5 @@ files: - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi - - - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz + - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz.tbi From 8ef09d30137e502d9c6aa096bc4b5703ae450db6 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 10 Dec 2021 01:40:55 +0100 Subject: [PATCH 087/235] Fix: CI error in test_removeduplicates.yml --- tests/test_removeduplicates.yml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/test_removeduplicates.yml b/tests/test_removeduplicates.yml index 164bee21..a161584e 100644 --- a/tests/test_removeduplicates.yml +++ b/tests/test_removeduplicates.yml @@ -1,7 +1,7 @@ -- name: Run default pipeline +- name: Run pipeline with duplicate reads removed command: nextflow run main.nf -profile test,docker --remove_duplicates true tags: - - default + - removeduplicates - preprocessing files: - path: results/reports/multiqc_report.html From cc95d210a1f373aade28ca1dc53bab59d130925e Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 10 Dec 2021 01:56:55 +0100 Subject: [PATCH 088/235] Correction in recalibrate subworkflow --- subworkflows/nf-core/recalibrate.nf | 15 +-------------- 1 file changed, 1 insertion(+), 14 deletions(-) diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index e7f1635d..b1209684 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -34,22 +34,9 @@ workflow RECALIBRATE { bam_reports = Channel.empty() APPLYBQSR(bam, fasta, fai, dict, intervals) + bam_recalibrated = APPLYBQSR.out.bam ch_versions = ch_versions.mix(APPLYBQSR.out.versions.first()) - // STEP 4.5: MERGING AND INDEXING THE RECALIBRATED BAM FILES - if (params.no_intervals) { - bam_recalibrated = APPLYBQSR.out.bam - } else { - APPLYBQSR.out.bam.map{ meta, bam -> - meta.id = meta.id - [meta, bam] - }.groupTuple().set{bam_recalibrated_interval} - - SAMTOOLS_MERGE(bam_recalibrated_interval, fasta) - bam_recalibrated = SAMTOOLS_MERGE.out.bam - ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) - } - SAMTOOLS_INDEX(bam_recalibrated) bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) From b89657668b6b80a0b6b144c0b1f9da7c5bde0391 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 13 Dec 2021 22:16:35 +0100 Subject: [PATCH 089/235] Fix: CI tests --- .github/workflows/ci.yml | 2 +- tests/config/nextflow.config | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 63d19123..3161e7a6 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -29,7 +29,7 @@ jobs: test: - 'default' - 'annotation' - - 'removeduplicates' + # - 'removeduplicates' steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/tests/config/nextflow.config b/tests/config/nextflow.config index 74e1c2d5..6bd687bb 100644 --- a/tests/config/nextflow.config +++ b/tests/config/nextflow.config @@ -4,13 +4,13 @@ params { enable_conda = false singularity_pull_docker_container = false max_cpus = 2 - max_memory = 6.GB + max_memory = 16.GB max_time = 6.h } process { cpus = 2 - memory = 6.GB + memory = 16.GB time = 48.h } From 75279ccaf1bf283c3025eb9982343639e8f54013 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 13 Dec 2021 23:02:55 +0100 Subject: [PATCH 090/235] Fix: CI tests - attempt 2 --- tests/config/nextflow.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/config/nextflow.config b/tests/config/nextflow.config index 6bd687bb..cd8f67c1 100644 --- a/tests/config/nextflow.config +++ b/tests/config/nextflow.config @@ -1,5 +1,5 @@ params { - outdir = "output/" + outdir = "results/" publish_dir_mode = "copy" enable_conda = false singularity_pull_docker_container = false @@ -25,7 +25,7 @@ if ("$PROFILE" == "singularity") { } // Load test_data.config containing paths to test data -includeConfig 'test_data.config' +// includeConfig 'test_data.config' // Load modules.config for default module params includeConfig '../../conf/modules.config' From be8a80c91446e9dd793547a185fd242d20299833 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 13 Dec 2021 23:21:47 +0100 Subject: [PATCH 091/235] Fix: CI tests - attempt 3 --- .github/workflows/ci.yml | 2 +- tests/config/nextflow.config | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 3161e7a6..63d19123 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -29,7 +29,7 @@ jobs: test: - 'default' - 'annotation' - # - 'removeduplicates' + - 'removeduplicates' steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/tests/config/nextflow.config b/tests/config/nextflow.config index cd8f67c1..1241d7d8 100644 --- a/tests/config/nextflow.config +++ b/tests/config/nextflow.config @@ -4,13 +4,13 @@ params { enable_conda = false singularity_pull_docker_container = false max_cpus = 2 - max_memory = 16.GB + max_memory = 6.GB max_time = 6.h } process { cpus = 2 - memory = 16.GB + memory = 6.GB time = 48.h } From e635be7a30e9657328d9403614db318a19435a73 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Dec 2021 01:20:16 +0100 Subject: [PATCH 092/235] Fix: CI test errors --- conf/modules.config | 39 +++++++++++------------------ subworkflows/nf-core/recalibrate.nf | 10 +++----- tests/test_annotation.yml | 3 +++ tests/test_default.yml | 4 +-- tests/test_removeduplicates.yml | 4 +-- workflows/rnavar.nf | 3 +-- 6 files changed, 27 insertions(+), 36 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 469f5713..d914c807 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -75,9 +75,21 @@ params { publish_files = false } 'gatk_applybqsr' { - args = '--use-original-qualities --add-output-sam-program-record' - suffix = '.markdup.recalibrated' - publish_files = false + args = '--use-original-qualities --add-output-sam-program-record' + suffix = '.recal' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['bam':'', 'bai':''] + } + 'samtools_index_recalibrate' { + suffix = '.recal' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['bai':''] + } + 'samtools_stats_recalibrate' { + publish_by_meta = true + publish_dir = 'reports/samtools_stats' } 'gatk_haplotypecaller' { args = '-dont-use-soft-clipped-bases' @@ -93,27 +105,6 @@ params { args = '' publish_files = false } - // RECALIBRATE - 'applybqsr' { - suffix = '.recal' - publish_files = false - } - 'merge_bam_recalibrate' { - suffix = '.recal.merge' - publish_by_meta = true - publish_files = ['bam':'', 'bai':''] - publish_dir = 'preprocessing' - } - 'samtools_index_recalibrate' { - suffix = 'recalibrated' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bam':'', 'bai':''] - } - 'samtools_stats_recalibrate' { - publish_by_meta = true - publish_dir = 'reports/samtools_stats' - } 'gatk_mergevcfs' { suffix = '.haplotypecaller' publish_by_meta = true diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index b1209684..ec948135 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -4,15 +4,13 @@ ======================================================================================== */ -params.applybqsr_options = [:] -params.merge_bam_options = [:] -params.qualimap_bamqc_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] +params.applybqsr_options = [:] +params.qualimap_bamqc_options = [:] +params.samtools_index_options = [:] +params.samtools_stats_options = [:] include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) -include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.merge_bam_options) include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) workflow RECALIBRATE { diff --git a/tests/test_annotation.yml b/tests/test_annotation.yml index 5ccd131b..d8855dc6 100644 --- a/tests/test_annotation.yml +++ b/tests/test_annotation.yml @@ -6,6 +6,7 @@ files: - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi + - path: results/reports/multiqc_report.html - name: Run VEP command: nextflow run main.nf -profile test,docker --annotate_tools vep tags: @@ -14,6 +15,7 @@ files: - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz.tbi + - path: results/reports/multiqc_report.html - name: Run snpEff followed by VEP command: nextflow run main.nf -profile test,docker --annotate_tools merge tags: @@ -26,3 +28,4 @@ - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz.tbi + - path: results/reports/multiqc_report.html diff --git a/tests/test_default.yml b/tests/test_default.yml index 508d5aa3..3a0c6847 100644 --- a/tests/test_default.yml +++ b/tests/test_default.yml @@ -5,8 +5,8 @@ - preprocessing files: - path: results/reports/multiqc_report.html - - path: results/preprocessing/GM12878/GM12878.recal.merge.bam - - path: results/preprocessing/GM12878/GM12878.recal.merge.bam.bai + - path: results/preprocessing/GM12878/GM12878.recal.bam + - path: results/preprocessing/GM12878/GM12878.recal.bam.bai - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz diff --git a/tests/test_removeduplicates.yml b/tests/test_removeduplicates.yml index a161584e..08619c83 100644 --- a/tests/test_removeduplicates.yml +++ b/tests/test_removeduplicates.yml @@ -5,8 +5,8 @@ - preprocessing files: - path: results/reports/multiqc_report.html - - path: results/preprocessing/GM12878/GM12878.recal.merge.bam - - path: results/preprocessing/GM12878/GM12878.recal.merge.bam.bai + - path: results/preprocessing/GM12878/GM12878.recal.bam + - path: results/preprocessing/GM12878/GM12878.recal.bam.bai - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index fe5eb00a..e2a18367 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -167,8 +167,7 @@ include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' // Estimate and correct systematic bias include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( - applybqsr_options: modules['applybqsr'], - merge_bam_options: modules['merge_bam_recalibrate'], + applybqsr_options: modules['gatk_applybqsr'], samtools_index_options: modules['samtools_index_recalibrate'], samtools_stats_options: modules['samtools_stats_recalibrate'] ) From b898bca04957bae2e96ad7fa6b78f74e7741d4d5 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Dec 2021 01:24:33 +0100 Subject: [PATCH 093/235] Fix: CI test errors --- nextflow.config | 2 +- nextflow_schema.json | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index d269b8d0..f2f4cff2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -58,7 +58,7 @@ params { skip_variantannotation = false // GATK intervallist parameters - scatter_count = 50 + scatter_count = 25 //GATK haplotypecaller parameters stand_call_conf = 20 diff --git a/nextflow_schema.json b/nextflow_schema.json index e73e0d06..5ab8c5cc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -364,7 +364,7 @@ "properties": { "scatter_count": { "type": "integer", - "default": 50, + "default": 25, "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", "help_text": "Set this parameter to decide the number of splits for the gene interval list file." }, From f683003c6d14de6474f57f1592a50cf677a82415 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Dec 2021 02:41:59 +0100 Subject: [PATCH 094/235] Fix: Merge annotation test --- subworkflows/local/annotate.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/annotate.nf b/subworkflows/local/annotate.nf index ff5b76b1..796fda54 100644 --- a/subworkflows/local/annotate.nf +++ b/subworkflows/local/annotate.nf @@ -63,7 +63,7 @@ workflow ANNOTATE { } if ('merge' in tools) { - vcf_ann_for_merge = snpeff_vcf_ann.map{ meta, vcf, tbi -> [meta, vcf] } + vcf_ann_for_merge = snpeff_vcf_ann.map{ meta, vcf, tbi -> [meta, vcf, tbi] } (merge_vcf_ann, merge_vep_report, merge_vep_version) = MERGE_ANNOTATE(vcf_ann_for_merge, vep_genome, vep_species, vep_cache_version, vep_cache) ch_versions = ch_versions.mix(MERGE_ANNOTATE.out.versions.first()) } From 698a7e202716078e56e890a37286cfec53fa5ffb Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 14 Dec 2021 16:19:08 +0100 Subject: [PATCH 095/235] Added rnavar custom config profile --- nextflow.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow.config b/nextflow.config index f2f4cff2..75247f70 100644 --- a/nextflow.config +++ b/nextflow.config @@ -114,11 +114,11 @@ includeConfig 'conf/base.config' // Load modules.config for DSL2 module specific options includeConfig 'conf/modules.config' -// Load nf-core custom profiles from different Institutions +// Load nf-core/rnavar custom profiles from different Institutions try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" + includeConfig "${params.custom_config_base}/pipeline/rnavar.config" } catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") + System.err.println("WARNING: Could not load nf-core/config/rnavar profiles: ${params.custom_config_base}/pipeline/rnavar.config") } // Load igenomes.config if required From 24af2b87931adb1227f0a24b741e22819621ac74 Mon Sep 17 00:00:00 2001 From: praveenraj2018 <43108054+praveenraj2018@users.noreply.github.com> Date: Tue, 14 Dec 2021 16:23:03 +0100 Subject: [PATCH 096/235] fix: lint error --- nextflow.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 75247f70..a8bc3778 100644 --- a/nextflow.config +++ b/nextflow.config @@ -116,9 +116,9 @@ includeConfig 'conf/modules.config' // Load nf-core/rnavar custom profiles from different Institutions try { - includeConfig "${params.custom_config_base}/pipeline/rnavar.config" + includeConfig "${params.custom_config_base}/pipeline/rnavar.config" } catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config/rnavar profiles: ${params.custom_config_base}/pipeline/rnavar.config") + System.err.println("WARNING: Could not load nf-core/config/rnavar profiles: ${params.custom_config_base}/pipeline/rnavar.config") } // Load igenomes.config if required From 6420e7bf45b5f4c56b9acdd94e26597a4e9be64a Mon Sep 17 00:00:00 2001 From: "Maxime U. Garcia" Date: Tue, 14 Dec 2021 16:30:45 +0100 Subject: [PATCH 097/235] Update nextflow.config --- nextflow.config | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/nextflow.config b/nextflow.config index a8bc3778..e1a0762a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -114,6 +114,12 @@ includeConfig 'conf/base.config' // Load modules.config for DSL2 module specific options includeConfig 'conf/modules.config' +// Load nf-core custom profiles from different Institutions +try { + includeConfig "${params.custom_config_base}/nfcore_custom.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") +} // Load nf-core/rnavar custom profiles from different Institutions try { includeConfig "${params.custom_config_base}/pipeline/rnavar.config" From 9b29fc13cc977c9bc7f9a787fab40d87e3a07b9f Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 3 Jan 2022 14:23:57 +0100 Subject: [PATCH 098/235] All modules updated to DSL2 syntax --- modules.json | 62 ++++++------ .../nf-core/modules/cat/fastq/functions.nf | 78 -------------- modules/nf-core/modules/cat/fastq/main.nf | 36 +++---- .../custom/dumpsoftwareversions/functions.nf | 78 -------------- .../custom/dumpsoftwareversions/main.nf | 95 +----------------- .../custom/dumpsoftwareversions/meta.yml | 1 + .../templates/dumpsoftwareversions.py | 89 ++++++++++++++++ .../nf-core/modules/ensemblvep/functions.nf | 78 -------------- modules/nf-core/modules/ensemblvep/main.nf | 37 ++----- modules/nf-core/modules/fastqc/functions.nf | 78 -------------- modules/nf-core/modules/fastqc/main.nf | 32 ++---- .../modules/gatk4/applybqsr/functions.nf | 78 -------------- .../nf-core/modules/gatk4/applybqsr/main.nf | 43 ++++---- .../nf-core/modules/gatk4/applybqsr/meta.yml | 7 +- .../gatk4/baserecalibrator/functions.nf | 78 -------------- .../modules/gatk4/baserecalibrator/main.nf | 37 +++---- .../modules/gatk4/baserecalibrator/meta.yml | 6 +- .../gatk4/bedtointervallist/functions.nf | 78 -------------- .../modules/gatk4/bedtointervallist/main.nf | 36 +++---- .../createsequencedictionary/functions.nf | 78 -------------- .../gatk4/createsequencedictionary/main.nf | 28 ++---- .../gatk4/indexfeaturefile/functions.nf | 78 -------------- .../modules/gatk4/indexfeaturefile/main.nf | 34 +++---- .../gatk4/intervallisttools/functions.nf | 78 -------------- .../modules/gatk4/intervallisttools/main.nf | 36 +++---- .../modules/gatk4/mergevcfs/functions.nf | 78 -------------- .../nf-core/modules/gatk4/mergevcfs/main.nf | 36 +++---- .../gatk4/variantfiltration/functions.nf | 78 -------------- .../modules/gatk4/variantfiltration/main.nf | 30 ++---- modules/nf-core/modules/gffread/functions.nf | 78 -------------- modules/nf-core/modules/gffread/main.nf | 26 ++--- modules/nf-core/modules/gunzip/functions.nf | 78 -------------- modules/nf-core/modules/gunzip/main.nf | 24 ++--- modules/nf-core/modules/gunzip/test.txt.gz | Bin 47 -> 0 bytes modules/nf-core/modules/multiqc/functions.nf | 78 -------------- modules/nf-core/modules/multiqc/main.nf | 24 ++--- .../picard/markduplicates/functions.nf | 78 -------------- .../modules/picard/markduplicates/main.nf | 28 ++---- .../modules/samtools/faidx/functions.nf | 78 -------------- .../nf-core/modules/samtools/faidx/main.nf | 28 ++---- .../nf-core/modules/samtools/faidx/meta.yml | 10 ++ .../modules/samtools/flagstat/functions.nf | 78 -------------- .../nf-core/modules/samtools/flagstat/main.nf | 22 ++-- .../modules/samtools/idxstats/functions.nf | 78 -------------- .../nf-core/modules/samtools/idxstats/main.nf | 22 ++-- .../modules/samtools/index/functions.nf | 78 -------------- .../nf-core/modules/samtools/index/main.nf | 26 ++--- .../modules/samtools/merge/functions.nf | 78 -------------- .../nf-core/modules/samtools/merge/main.nf | 26 ++--- .../modules/samtools/sort/functions.nf | 78 -------------- modules/nf-core/modules/samtools/sort/main.nf | 26 ++--- .../modules/samtools/stats/functions.nf | 78 -------------- .../nf-core/modules/samtools/stats/main.nf | 22 ++-- modules/nf-core/modules/snpeff/functions.nf | 78 -------------- modules/nf-core/modules/snpeff/main.nf | 42 +++----- .../nf-core/modules/star/align/functions.nf | 78 -------------- modules/nf-core/modules/star/align/main.nf | 39 +++---- .../modules/star/genomegenerate/functions.nf | 78 -------------- .../modules/star/genomegenerate/main.nf | 34 +++---- .../modules/tabix/bgziptabix/functions.nf | 78 -------------- .../nf-core/modules/tabix/bgziptabix/main.nf | 31 ++---- modules/nf-core/modules/untar/functions.nf | 78 -------------- modules/nf-core/modules/untar/main.nf | 28 ++---- nf-core/modules/tabix/bgziptabix/main.nf | 39 +++++++ nf-core/modules/tabix/bgziptabix/meta.yml | 45 +++++++++ 65 files changed, 521 insertions(+), 2850 deletions(-) delete mode 100644 modules/nf-core/modules/cat/fastq/functions.nf delete mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf create mode 100644 modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/modules/ensemblvep/functions.nf delete mode 100644 modules/nf-core/modules/fastqc/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/applybqsr/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/baserecalibrator/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/bedtointervallist/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/intervallisttools/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/mergevcfs/functions.nf delete mode 100644 modules/nf-core/modules/gatk4/variantfiltration/functions.nf delete mode 100644 modules/nf-core/modules/gffread/functions.nf delete mode 100644 modules/nf-core/modules/gunzip/functions.nf delete mode 100644 modules/nf-core/modules/gunzip/test.txt.gz delete mode 100644 modules/nf-core/modules/multiqc/functions.nf delete mode 100644 modules/nf-core/modules/picard/markduplicates/functions.nf delete mode 100644 modules/nf-core/modules/samtools/faidx/functions.nf delete mode 100644 modules/nf-core/modules/samtools/flagstat/functions.nf delete mode 100644 modules/nf-core/modules/samtools/idxstats/functions.nf delete mode 100644 modules/nf-core/modules/samtools/index/functions.nf delete mode 100644 modules/nf-core/modules/samtools/merge/functions.nf delete mode 100644 modules/nf-core/modules/samtools/sort/functions.nf delete mode 100644 modules/nf-core/modules/samtools/stats/functions.nf delete mode 100644 modules/nf-core/modules/snpeff/functions.nf delete mode 100644 modules/nf-core/modules/star/align/functions.nf delete mode 100644 modules/nf-core/modules/star/genomegenerate/functions.nf delete mode 100644 modules/nf-core/modules/tabix/bgziptabix/functions.nf delete mode 100644 modules/nf-core/modules/untar/functions.nf create mode 100644 nf-core/modules/tabix/bgziptabix/main.nf create mode 100644 nf-core/modules/tabix/bgziptabix/meta.yml diff --git a/modules.json b/modules.json index 0e780207..b8f829e5 100644 --- a/modules.json +++ b/modules.json @@ -4,88 +4,88 @@ "repos": { "nf-core/modules": { "cat/fastq": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "826a5603db5cf5b4f1e55cef9cc0b7c37d3c7e70" }, "custom/dumpsoftwareversions": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "ensemblvep": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" }, "fastqc": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" }, "gatk4/applybqsr": { - "git_sha": "34268347447cd60013b69279a42aa9d081592735" + "git_sha": "a6e0629e2430a9d4061ddc8afdf4cff8c93080b5" }, "gatk4/baserecalibrator": { - "git_sha": "34268347447cd60013b69279a42aa9d081592735" + "git_sha": "a6e0629e2430a9d4061ddc8afdf4cff8c93080b5" }, "gatk4/bedtointervallist": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gatk4/createsequencedictionary": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gatk4/indexfeaturefile": { - "git_sha": "1a4c7cec1b9d82fdaa15897d8e9a9e9a4767444d" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gatk4/intervallisttools": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gatk4/mergevcfs": { - "git_sha": "3b600af50eae8264960df817277cfe303d2acd47" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gatk4/variantfiltration": { - "git_sha": "43a1c1c6d18d7d9395846503046f1649ba72a06e" + "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" }, "gffread": { - "git_sha": "49da8642876ae4d91128168cd0db4f1c858d7792" + "git_sha": "d473a247d2e0c619b0df877ea19d9a5a98c8e3c8" }, "gunzip": { - "git_sha": "d30bf235b1524327703591c9ce2f372bfb2d474f" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "multiqc": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "picard/markduplicates": { - "git_sha": "4e5b6ed843ee0691848aba58088e4347cd3aae98" + "git_sha": "0d1e21686a586447b7592e40da9b3a7cdeedf03c" }, "samtools/faidx": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "e0aa89141ffecb5f54d230f7ea46de242b74e084" }, "samtools/flagstat": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "samtools/idxstats": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "samtools/index": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "0fafaeebf52cc5ab554b83297ed02a48d852a848" }, "samtools/merge": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "d473a247d2e0c619b0df877ea19d9a5a98c8e3c8" }, "samtools/sort": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" }, "samtools/stats": { - "git_sha": "19035c99d1cd8412b5046c6bb4af787658b84e5b" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "snpeff": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" }, "star/align": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" - }, + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + }, "star/genomegenerate": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" - }, - "tabix/bgziptabix": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, + "tabix/bgziptabix": { + "git_sha": "123" + }, "untar": { - "git_sha": "3aacd46da2b221ed47aaa05c413a828538d2c2ae" + "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" } } } diff --git a/modules/nf-core/modules/cat/fastq/functions.nf b/modules/nf-core/modules/cat/fastq/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/cat/fastq/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf index 538915a7..d02598e1 100644 --- a/modules/nf-core/modules/cat/fastq/main.nf +++ b/modules/nf-core/modules/cat/fastq/main.nf @@ -1,41 +1,31 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process CAT_FASTQ { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : + 'biocontainers/biocontainers:v1.2.0_cv1' }" input: - tuple val(meta), path(reads) + tuple val(meta), path(reads, stageAs: "input*/*") output: tuple val(meta), path("*.merged.fastq.gz"), emit: reads path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def readList = reads.collect{ it.toString() } if (meta.single_end) { if (readList.size > 1) { """ - cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + cat ${readList.join(' ')} > ${prefix}.merged.fastq.gz cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') + "${task.process}": + cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') END_VERSIONS """ } @@ -45,12 +35,12 @@ process CAT_FASTQ { def read2 = [] readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } """ - cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz - cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + cat ${read1.join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.join(' ')} > ${prefix}_2.merged.fastq.gz cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') + "${task.process}": + cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf index faf2073f..934bb467 100644 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda (params.enable_conda ? "bioconda::multiqc=1.11" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" - } else { - container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }" input: path versions @@ -27,80 +16,6 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { path "versions.yml" , emit: versions script: - """ - #!/usr/bin/env python - - import yaml - import platform - from textwrap import dedent - - def _make_versions_html(versions): - html = [ - dedent( - '''\\ - - - - - - - - - - ''' - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f'''\\ - - - - - - ''' - ) - ) - html.append("") - html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") - return "\\n".join(html) - - module_versions = {} - module_versions["${getProcessName(task.process)}"] = { - 'python': platform.python_version(), - 'yaml': yaml.__version__ - } - - with open("$versions") as f: - workflow_versions = yaml.load(f, Loader=yaml.BaseLoader) | module_versions - - workflow_versions["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version" - } - - versions_mqc = { - 'id': 'software_versions', - 'section_name': '${workflow.manifest.name} Software Versions', - 'section_href': 'https://github.com/${workflow.manifest.name}', - 'plot_type': 'html', - 'description': 'are collected at run time from the software output.', - 'data': _make_versions_html(workflow_versions) - } - - with open("software_versions.yml", 'w') as f: - yaml.dump(workflow_versions, f, default_flow_style=False) - with open("software_versions_mqc.yml", 'w') as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open('versions.yml', 'w') as f: - yaml.dump(module_versions, f, default_flow_style=False) - """ + def args = task.ext.args ?: '' + template 'dumpsoftwareversions.py' } diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml index c8310e35..5b5b8a60 100644 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml @@ -31,3 +31,4 @@ output: authors: - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py new file mode 100644 index 00000000..d1390392 --- /dev/null +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -0,0 +1,89 @@ +#!/usr/bin/env python + +import yaml +import platform +from textwrap import dedent + + +def _make_versions_html(versions): + html = [ + dedent( + """\\ + + + + + + + + + + """ + ) + ] + for process, tmp_versions in sorted(versions.items()): + html.append("") + for i, (tool, version) in enumerate(sorted(tmp_versions.items())): + html.append( + dedent( + f"""\\ + + + + + + """ + ) + ) + html.append("") + html.append("
Process Name Software Version
{process if (i == 0) else ''}{tool}{version}
") + return "\\n".join(html) + + +versions_this_module = {} +versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, +} + +with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + +# aggregate versions by the module name (derived from fully-qualified process name) +versions_by_module = {} +for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + assert versions_by_module[module] == process_versions, ( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + +versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", +} + +versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), +} + +with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) +with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + +with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) diff --git a/modules/nf-core/modules/ensemblvep/functions.nf b/modules/nf-core/modules/ensemblvep/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/ensemblvep/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/ensemblvep/main.nf b/modules/nf-core/modules/ensemblvep/main.nf index 282dcddd..e3d0c286 100644 --- a/modules/nf-core/modules/ensemblvep/main.nf +++ b/modules/nf-core/modules/ensemblvep/main.nf @@ -1,30 +1,14 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) -params.use_cache = false -params.vep_tag = "" - process ENSEMBLVEP { + tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::ensembl-vep=104.3" : null) - if (params.use_cache) { - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/ensembl-vep:104.3--pl5262h4a94de4_0" - } else { - container "quay.io/biocontainers/ensembl-vep:104.3--pl5262h4a94de4_0" - } - } else { - container "nfcore/vep:${params.vep_tag}" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ensembl-vep:104.3--pl5262h4a94de4_0' : + 'quay.io/biocontainers/ensembl-vep:104.3--pl5262h4a94de4_0' }" input: - tuple val(meta), path(vcf), path(tbi) + tuple val(meta), path(vcf) val genome val species val cache_version @@ -36,15 +20,16 @@ process ENSEMBLVEP { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - dir_cache = params.use_cache ? "\${PWD}/${cache}" : "/.vep" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def dir_cache = cache ? "\${PWD}/${cache}" : "/.vep" """ mkdir $prefix vep \\ -i $vcf \\ -o ${prefix}.ann.vcf \\ - $options.args \\ + $args \\ --assembly $genome \\ --species $species \\ --cache \\ @@ -57,8 +42,8 @@ process ENSEMBLVEP { rm -rf $prefix cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') + "${task.process}": + ensemblvep: \$( echo \$(vep --help 2>&1) | sed 's/^.*Versions:.*ensembl-vep : //;s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/fastqc/functions.nf b/modules/nf-core/modules/fastqc/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/fastqc/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf index 9f6cfc55..d250eca0 100644 --- a/modules/nf-core/modules/fastqc/main.nf +++ b/modules/nf-core/modules/fastqc/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process FASTQC { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" - } else { - container "quay.io/biocontainers/fastqc:0.11.9--0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : + 'quay.io/biocontainers/fastqc:0.11.9--0' }" input: tuple val(meta), path(reads) @@ -27,27 +16,28 @@ process FASTQC { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' // Add soft-links to original FastQs for consistent naming in pipeline - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.id}" if (meta.single_end) { """ [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz - fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz + fastqc $args --threads $task.cpus ${prefix}.fastq.gz cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$( fastqc --version | sed -e "s/FastQC v//g" ) + "${task.process}": + fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) END_VERSIONS """ } else { """ [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz - fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz + fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$( fastqc --version | sed -e "s/FastQC v//g" ) + "${task.process}": + fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/applybqsr/functions.nf b/modules/nf-core/modules/gatk4/applybqsr/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/applybqsr/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index c89a4a4d..22702211 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -1,55 +1,48 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: - tuple val(meta), path(input), path(input_index), path(bqsr_table) + tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals) path fasta path fai path dict - path intervals output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions + tuple val(meta), path("*.bam"), emit: bam, optional: true + tuple val(meta), path("*.cram"), emit: cram, optional: true + path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def interval = intervals ? "-L ${intervals}" : "" + def file_type = input.getExtension() + + def avail_mem = 3 if (!task.memory) { log.info '[GATK ApplyBQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ - gatk ApplyBQSR \\ + gatk --java-options "-Xmx${avail_mem}g" ApplyBQSR \\ -R $fasta \\ -I $input \\ --bqsr-recal-file $bqsr_table \\ $interval \\ --tmp-dir . \\ - -O ${prefix}.bam \\ - $options.args + -O ${prefix}.${file_type} \\ + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/applybqsr/meta.yml b/modules/nf-core/modules/gatk4/applybqsr/meta.yml index 4e3b2f9a..ad1f82a1 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/meta.yml +++ b/modules/nf-core/modules/gatk4/applybqsr/meta.yml @@ -31,6 +31,9 @@ input: - bqsr_table: type: file description: Recalibration table from gatk4_baserecalibrator + - intervals: + type: file + description: Bed file with the genomic regions included in the library (optional) - fasta: type: file description: The reference fasta file @@ -43,9 +46,7 @@ input: type: file description: GATK sequence dictionary pattern: "*.dict" - - intervalsBed: - type: file - description: Bed file with the genomic regions included in the library (optional) + output: - meta: diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf b/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/baserecalibrator/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index ce6f5906..3a26d6cc 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -1,29 +1,17 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_BASERECALIBRATOR { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: - tuple val(meta), path(input), path(input_index) + tuple val(meta), path(input), path(input_index), path(intervals) path fasta path fai path dict - path intervalsBed path knownSites path knownSites_tbi @@ -32,28 +20,31 @@ process GATK4_BASERECALIBRATOR { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def intervalsCommand = intervalsBed ? "-L ${intervalsBed}" : "" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def intervalsCommand = intervals ? "-L ${intervals}" : "" def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') + def avail_mem = 3 if (!task.memory) { log.info '[GATK BaseRecalibrator] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } + """ - gatk BaseRecalibrator \ + gatk --java-options "-Xmx${avail_mem}g" BaseRecalibrator \ -R $fasta \ -I $input \ $sitesCommand \ $intervalsCommand \ --tmp-dir . \ - $options.args \ + $args \ -O ${prefix}.table cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml index 188340b4..641a50df 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml +++ b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml @@ -28,6 +28,9 @@ input: type: file description: BAI/CRAI file from alignment pattern: "*.{bai,crai}" + - intervals: + type: file + description: Bed file with the genomic regions included in the library (optional) - fasta: type: file description: The reference fasta file @@ -40,9 +43,6 @@ input: type: file description: GATK sequence dictionary pattern: "*.dict" - - intervalsBed: - type: file - description: Bed file with the genomic regions included in the library (optional) - knownSites: type: file description: Bed file with the genomic regions included in the library (optional) diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf b/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/bedtointervallist/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index 7c06ccef..76d0f8a4 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_BEDTOINTERVALLIST { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: tuple val(meta), path(bed) @@ -27,17 +16,24 @@ process GATK4_BEDTOINTERVALLIST { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK BedToIntervalList] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } """ - gatk BedToIntervalList \\ + gatk --java-options "-Xmx${avail_mem}g" BedToIntervalList \\ -I $bed \\ -SD $sequence_dict \\ -O ${prefix}.interval_list \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index db28e244..4d82a643 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_CREATESEQUENCEDICTIONARY { tag "$fasta" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: path fasta @@ -26,9 +15,10 @@ process GATK4_CREATESEQUENCEDICTIONARY { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' def avail_mem = 6 if (!task.memory) { - log.info '[GATK] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' + log.info '[GATK CreateSequenceDictionary] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } @@ -37,11 +27,11 @@ process GATK4_CREATESEQUENCEDICTIONARY { CreateSequenceDictionary \\ --REFERENCE $fasta \\ --URI $fasta \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index 8f40a3e3..63633e5f 100644 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_INDEXFEATUREFILE { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0" - } else { - container "quay.io/biocontainers/gatk4:4.2.0.0--0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: tuple val(meta), path(feature_file) @@ -26,15 +15,22 @@ process GATK4_INDEXFEATUREFILE { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK IndexFeatureFile] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } """ - gatk \\ + gatk --java-options "-Xmx${avail_mem}g" \\ IndexFeatureFile \\ - $options.args \\ + $args \\ -I $feature_file cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/intervallisttools/functions.nf b/modules/nf-core/modules/gatk4/intervallisttools/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/intervallisttools/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index 5da651b9..b1881932 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_INTERVALLISTTOOLS { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: tuple val(meta), path(interval_list) @@ -26,16 +15,23 @@ process GATK4_INTERVALLISTTOOLS { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK IntervalListTools] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } """ mkdir ${prefix}_split - gatk \\ + gatk --java-options "-Xmx${avail_mem}g" \\ IntervalListTools \\ -I ${interval_list} \\ -O ${prefix}_split \\ - $options.args + $args python3 < versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/mergevcfs/functions.nf b/modules/nf-core/modules/gatk4/mergevcfs/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/mergevcfs/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf index 28073fcb..9158a242 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_MERGEVCFS { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: tuple val(meta), path(vcfs) @@ -28,7 +17,8 @@ process GATK4_MERGEVCFS { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" // Make list of VCFs to merge def input = "" @@ -36,16 +26,22 @@ process GATK4_MERGEVCFS { input += " I=${vcf}" } def ref = use_ref_dict ? "D=${ref_dict}" : "" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK MergeVcfs] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } """ - gatk MergeVcfs \\ + gatk --java-options "-Xmx${avail_mem}g" MergeVcfs \\ $input \\ O=${prefix}.vcf.gz \\ $ref \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gatk4/variantfiltration/functions.nf b/modules/nf-core/modules/gatk4/variantfiltration/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gatk4/variantfiltration/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index e0f0727a..32e4aae1 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_VARIANTFILTRATION { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" input: tuple val(meta), path(vcf), path(vcf_tbi) @@ -30,10 +19,11 @@ process GATK4_VARIANTFILTRATION { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def avail_mem = 3 if (!task.memory) { - log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + log.info '[GATK VariantFiltration] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.toGiga() } @@ -42,11 +32,11 @@ process GATK4_VARIANTFILTRATION { -R $fasta \\ -V $vcf \\ -O ${prefix}.vcf.gz \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gffread/functions.nf b/modules/nf-core/modules/gffread/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gffread/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gffread/main.nf b/modules/nf-core/modules/gffread/main.nf index 4133ee08..e7893f8b 100644 --- a/modules/nf-core/modules/gffread/main.nf +++ b/modules/nf-core/modules/gffread/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GFFREAD { tag "$gff" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "bioconda::gffread=0.12.1" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0" - } else { - container "quay.io/biocontainers/gffread:0.12.1--h8b12597_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' : + 'quay.io/biocontainers/gffread:0.12.1--h8b12597_0' }" input: path gff @@ -26,15 +15,16 @@ process GFFREAD { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${gff.baseName}${options.suffix}" : "${gff.baseName}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${gff.baseName}" """ gffread \\ $gff \\ - $options.args \\ + $args \\ -o ${prefix}.gtf cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(gffread --version 2>&1) + "${task.process}": + gffread: \$(gffread --version 2>&1) END_VERSIONS """ } diff --git a/modules/nf-core/modules/gunzip/functions.nf b/modules/nf-core/modules/gunzip/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/gunzip/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf index 564fa99d..77a4e546 100644 --- a/modules/nf-core/modules/gunzip/main.nf +++ b/modules/nf-core/modules/gunzip/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GUNZIP { tag "$archive" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : + 'biocontainers/biocontainers:v1.2.0_cv1' }" input: tuple val(meta), path(archive) @@ -26,16 +15,17 @@ process GUNZIP { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' gunzip = archive.toString() - '.gz' """ gunzip \\ -f \\ - $options.args \\ + $args \\ $archive cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') + "${task.process}": + gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/gunzip/test.txt.gz b/modules/nf-core/modules/gunzip/test.txt.gz deleted file mode 100644 index 381417cf643f1b5c547b57b251d71e6d5ce11e16..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 47 zcmb2|=HU3lo{`AFT#{N`qE}K;!r-m#=Xv^+o}cIW6JE^0nUR_|V;IhR&VMY%z`y_i DevlAG diff --git a/modules/nf-core/modules/multiqc/functions.nf b/modules/nf-core/modules/multiqc/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/multiqc/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf index 0861aa59..3dceb162 100644 --- a/modules/nf-core/modules/multiqc/main.nf +++ b/modules/nf-core/modules/multiqc/main.nf @@ -1,21 +1,10 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process MULTIQC { label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0" - } else { - container "quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }" input: path multiqc_files @@ -27,12 +16,13 @@ process MULTIQC { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' """ - multiqc -f $options.args . + multiqc -f $args . cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) END_VERSIONS """ } diff --git a/modules/nf-core/modules/picard/markduplicates/functions.nf b/modules/nf-core/modules/picard/markduplicates/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/picard/markduplicates/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index 130a1e52..3087bff4 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process PICARD_MARKDUPLICATES { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? 'bioconda::picard=2.25.7' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/picard:2.25.7--hdfd78af_0" - } else { - container "quay.io/biocontainers/picard:2.25.7--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::picard=2.26.7" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/picard:2.26.7--hdfd78af_0' : + 'quay.io/biocontainers/picard:2.26.7--hdfd78af_0' }" input: tuple val(meta), path(bam) @@ -28,7 +17,8 @@ process PICARD_MARKDUPLICATES { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def avail_mem = 3 if (!task.memory) { log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -39,14 +29,14 @@ process PICARD_MARKDUPLICATES { picard \\ -Xmx${avail_mem}g \\ MarkDuplicates \\ - $options.args \\ + $args \\ I=$bam \\ O=${prefix}.bam \\ M=${prefix}.MarkDuplicates.metrics.txt cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) + "${task.process}": + picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/faidx/functions.nf b/modules/nf-core/modules/samtools/faidx/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/faidx/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index 80708084..d8308b03 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -1,36 +1,26 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_FAIDX { tag "$fasta" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: - path fasta + tuple val(meta), path(fasta) output: - path "*.fai" , emit: fai - path "versions.yml", emit: versions + tuple val(meta), path ("*.fai") , emit: fai + path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' """ samtools faidx $fasta cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/faidx/meta.yml b/modules/nf-core/modules/samtools/faidx/meta.yml index 16c0b334..bae97a39 100644 --- a/modules/nf-core/modules/samtools/faidx/meta.yml +++ b/modules/nf-core/modules/samtools/faidx/meta.yml @@ -14,11 +14,21 @@ tools: doi: 10.1093/bioinformatics/btp352 licence: ['MIT'] input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - fasta: type: file description: FASTA file pattern: "*.{fa,fasta}" output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - fai: type: file description: FASTA index file diff --git a/modules/nf-core/modules/samtools/flagstat/functions.nf b/modules/nf-core/modules/samtools/flagstat/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/flagstat/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index 072a135f..03721d0b 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(bam), path(bai) @@ -26,11 +15,12 @@ process SAMTOOLS_FLAGSTAT { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' """ samtools flagstat --threads ${task.cpus-1} $bam > ${bam}.flagstat cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/idxstats/functions.nf b/modules/nf-core/modules/samtools/idxstats/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/idxstats/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index fa0e7dc3..cd068679 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(bam), path(bai) @@ -26,11 +15,12 @@ process SAMTOOLS_IDXSTATS { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' """ samtools idxstats $bam > ${bam}.idxstats cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/index/functions.nf b/modules/nf-core/modules/samtools/index/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/index/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index d66e4513..db025a8f 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -1,39 +1,29 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(input) output: tuple val(meta), path("*.bai") , optional:true, emit: bai - tuple val(meta), path("*.crai"), optional:true, emit: crai tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai"), optional:true, emit: crai path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' """ - samtools index -@ ${task.cpus-1} $options.args $input + samtools index -@ ${task.cpus-1} $args $input cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/merge/functions.nf b/modules/nf-core/modules/samtools/merge/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/merge/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index ab641bb9..fcfcf61f 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_MERGE { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(input_files) @@ -28,15 +17,16 @@ process SAMTOOLS_MERGE { path "versions.yml" , emit: versions script: - prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" def file_type = input_files[0].getExtension() def reference = fasta ? "--reference ${fasta}" : "" """ - samtools merge --threads ${task.cpus-1} $options.args ${reference} ${prefix}.${file_type} $input_files + samtools merge --threads ${task.cpus-1} $args ${reference} ${prefix}.${file_type} $input_files cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/sort/functions.nf b/modules/nf-core/modules/samtools/sort/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/sort/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index f980b472..0c2cf25e 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(bam) @@ -26,12 +15,13 @@ process SAMTOOLS_SORT { path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" """ - samtools sort $options.args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam + samtools sort $args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/samtools/stats/functions.nf b/modules/nf-core/modules/samtools/stats/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/samtools/stats/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index e0a2b50d..83c87002 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process SAMTOOLS_STATS { tag "$meta.id" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : + 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" input: tuple val(meta), path(input), path(input_index) @@ -27,13 +16,14 @@ process SAMTOOLS_STATS { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' def reference = fasta ? "--reference ${fasta}" : "" """ samtools stats --threads ${task.cpus-1} ${reference} ${input} > ${input}.stats cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/snpeff/functions.nf b/modules/nf-core/modules/snpeff/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/snpeff/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/snpeff/main.nf b/modules/nf-core/modules/snpeff/main.nf index 7e8f9d73..6248fee3 100644 --- a/modules/nf-core/modules/snpeff/main.nf +++ b/modules/nf-core/modules/snpeff/main.nf @@ -1,30 +1,14 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) -params.use_cache = false -params.snpeff_tag = "" - process SNPEFF { + tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::snpeff=5.0" : null) - if (params.use_cache) { - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/snpeff:5.0--hdfd78af_1" - } else { - container "quay.io/biocontainers/snpeff:5.0--hdfd78af_1" - } - } else { - container "nfcore/snpeff:${params.snpeff_tag}" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/snpeff:5.0--hdfd78af_1' : + 'quay.io/biocontainers/snpeff:5.0--hdfd78af_1' }" input: - tuple val(meta), path(vcf), path(vcf_tbi) + tuple val(meta), path(vcf) val db path cache @@ -34,26 +18,28 @@ process SNPEFF { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' def avail_mem = 6 if (!task.memory) { log.info '[snpEff] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def dir_cache = params.use_cache ? "-dataDir \${PWD}/${cache}" : "" + def prefix = task.ext.prefix ?: "${meta.id}" + def cache_command = cache ? "-dataDir \${PWD}/${cache}" : "" """ - snpEff -Xmx${avail_mem}g \\ + snpEff \\ + -Xmx${avail_mem}g \\ $db \\ - $options.args \\ + $args \\ -csvStats ${prefix}.csv \\ - $dir_cache \\ + $cache_command \\ $vcf \\ > ${prefix}.ann.vcf cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(snpEff -version 2>&1) | cut -f 2 -d ' ') + "${task.process}": + snpeff: \$(echo \$(snpEff -version 2>&1) | cut -f 2 -d ' ') END_VERSIONS """ } diff --git a/modules/nf-core/modules/star/align/functions.nf b/modules/nf-core/modules/star/align/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/star/align/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf index e0ccba8c..9725496f 100644 --- a/modules/nf-core/modules/star/align/main.nf +++ b/modules/nf-core/modules/star/align/main.nf @@ -1,28 +1,20 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process STAR_ALIGN { tag "$meta.id" label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } // Note: 2.7X indices incompatible with AWS iGenomes. conda (params.enable_conda ? 'bioconda::star=2.7.9a' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' - } else { - container 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/star:2.7.9a--h9ee0642_0' : + 'quay.io/biocontainers/star:2.7.9a--h9ee0642_0' }" input: tuple val(meta), path(reads) path index path gtf + val star_ignore_sjdbgtf + val seq_platform + val seq_center output: tuple val(meta), path('*d.out.bam') , emit: bam @@ -39,12 +31,13 @@ process STAR_ALIGN { tuple val(meta), path('*.out.junction') , optional:true, emit: junction script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" - def seq_platform = params.seq_platform ? "'PL:$params.seq_platform'" : "" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform " - def out_sam_type = (options.args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' - def mv_unsorted_bam = (options.args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def ignore_gtf = star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" + def seq_platform = seq_platform ? "'PL:$seq_platform'" : "" + def seq_center = seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$seq_center' 'SM:$prefix' $seq_platform " : "--outSAMattrRGline ID:$prefix 'SM:$prefix' $seq_platform " + def out_sam_type = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' + def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' """ STAR \\ --genomeDir $index \\ @@ -54,7 +47,7 @@ process STAR_ALIGN { $out_sam_type \\ $ignore_gtf \\ $seq_center \\ - $options.args + $args $mv_unsorted_bam @@ -68,8 +61,8 @@ process STAR_ALIGN { fi cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + "${task.process}": + star: \$(STAR --version | sed -e "s/STAR_//g") END_VERSIONS """ } diff --git a/modules/nf-core/modules/star/genomegenerate/functions.nf b/modules/nf-core/modules/star/genomegenerate/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/star/genomegenerate/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf index c932fafe..ad32c0dd 100644 --- a/modules/nf-core/modules/star/genomegenerate/main.nf +++ b/modules/nf-core/modules/star/genomegenerate/main.nf @@ -1,23 +1,12 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process STAR_GENOMEGENERATE { tag "$fasta" label 'process_high' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) } // Note: 2.7X indices incompatible with AWS iGenomes. conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" - } else { - container "quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' : + 'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' }" input: path fasta @@ -28,9 +17,10 @@ process STAR_GENOMEGENERATE { path "versions.yml" , emit: versions script: + def args = task.ext.args ?: '' + def args_list = args.tokenize() def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' - def args = options.args.tokenize() - if (args.contains('--genomeSAindexNbases')) { + if (args_list.contains('--genomeSAindexNbases')) { """ mkdir star STAR \\ @@ -40,11 +30,11 @@ process STAR_GENOMEGENERATE { --sjdbGTFfile $gtf \\ --runThreadN $task.cpus \\ $memory \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + "${task.process}": + star: \$(STAR --version | sed -e "s/STAR_//g") samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//') END_VERSIONS @@ -63,11 +53,11 @@ process STAR_GENOMEGENERATE { --runThreadN $task.cpus \\ --genomeSAindexNbases \$NUM_BASES \\ $memory \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(STAR --version | sed -e "s/STAR_//g") + "${task.process}": + star: \$(STAR --version | sed -e "s/STAR_//g") samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') gawk: \$(echo \$(gawk --version 2>&1) | sed 's/^.*GNU Awk //; s/, .*\$//') END_VERSIONS diff --git a/modules/nf-core/modules/tabix/bgziptabix/functions.nf b/modules/nf-core/modules/tabix/bgziptabix/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/tabix/bgziptabix/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/tabix/bgziptabix/main.nf b/modules/nf-core/modules/tabix/bgziptabix/main.nf index e44a7226..e419d153 100644 --- a/modules/nf-core/modules/tabix/bgziptabix/main.nf +++ b/modules/nf-core/modules/tabix/bgziptabix/main.nf @@ -1,39 +1,30 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process TABIX_BGZIPTABIX { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? 'bioconda::tabix=1.11' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0" - } else { - container "quay.io/biocontainers/tabix:1.11--hdfd78af_0" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" input: tuple val(meta), path(input) output: - tuple val(meta), path("*.gz"), path("*.tbi"), emit: tbi + tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi path "versions.yml" , emit: versions script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" """ - bgzip -c $options.args $input > ${prefix}.gz - tabix $options.args2 ${prefix}.gz + bgzip -c $args $input > ${prefix}.gz + tabix $args2 ${prefix}.gz cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/modules/untar/functions.nf b/modules/nf-core/modules/untar/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/nf-core/modules/untar/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf index efb9d825..6d1996e7 100644 --- a/modules/nf-core/modules/untar/main.nf +++ b/modules/nf-core/modules/untar/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process UNTAR { tag "$archive" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img" - } else { - container "biocontainers/biocontainers:v1.2.0_cv1" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : + 'biocontainers/biocontainers:v1.2.0_cv1' }" input: path archive @@ -26,16 +15,19 @@ process UNTAR { path "versions.yml", emit: versions script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' untar = archive.toString() - '.tar.gz' """ tar \\ -xzvf \\ - $options.args \\ - $archive + $args \\ + $archive \\ + $args2 \\ cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') END_VERSIONS """ } diff --git a/nf-core/modules/tabix/bgziptabix/main.nf b/nf-core/modules/tabix/bgziptabix/main.nf new file mode 100644 index 00000000..e44a7226 --- /dev/null +++ b/nf-core/modules/tabix/bgziptabix/main.nf @@ -0,0 +1,39 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' + +params.options = [:] +options = initOptions(params.options) + +process TABIX_BGZIPTABIX { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } + + conda (params.enable_conda ? 'bioconda::tabix=1.11' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0" + } else { + container "quay.io/biocontainers/tabix:1.11--hdfd78af_0" + } + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.gz"), path("*.tbi"), emit: tbi + path "versions.yml" , emit: versions + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + """ + bgzip -c $options.args $input > ${prefix}.gz + tabix $options.args2 ${prefix}.gz + + cat <<-END_VERSIONS > versions.yml + ${getProcessName(task.process)}: + ${getSoftwareName(task.process)}: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/nf-core/modules/tabix/bgziptabix/meta.yml b/nf-core/modules/tabix/bgziptabix/meta.yml new file mode 100644 index 00000000..f2aed84d --- /dev/null +++ b/nf-core/modules/tabix/bgziptabix/meta.yml @@ -0,0 +1,45 @@ +name: tabix_bgziptabix +description: bgzip a sorted tab-delimited genome file and then create tabix index +keywords: + - bgzip + - compress + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file + pattern: "*.{bed,gff,sam,vcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gz: + type: file + description: Output compressed file + pattern: "*.{gz}" + - tbi: + type: file + description: tabix index file + pattern: "*.{gz.tbi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" From e6b9e18b120ec603e6b54d3575b19e1a465c579a Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 3 Jan 2022 14:24:35 +0100 Subject: [PATCH 099/235] Fix: lint error --- .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index c1a08712..13339b0b 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -36,7 +36,7 @@ body: attributes: label: Relevant files description: | - Please upload (drag and drop) and relevant files. Make into a `.zip` file if the extension is not allowed. + Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed. Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files. - type: textarea From 25e49ebb758c236e5b132568a23d6ab7a6c91177 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 4 Jan 2022 18:12:31 +0100 Subject: [PATCH 100/235] Update for DSL2 --- .nf-core.yml | 2 - conf/modules.config | 424 +++++++++++++------- conf/modules.config.bk | 170 ++++++++ modules.json | 10 +- modules/nf-core/modules/star/align/meta.yml | 81 ---- nf-core/modules/tabix/bgziptabix/main.nf | 39 -- nf-core/modules/tabix/bgziptabix/meta.yml | 45 --- subworkflows/local/annotate.nf | 33 +- subworkflows/local/prepare_genome.nf | 28 +- subworkflows/local/splitncigar.nf | 12 +- subworkflows/nf-core/align_star.nf | 25 +- subworkflows/nf-core/bam_sort_samtools.nf | 12 +- subworkflows/nf-core/bam_stats_samtools.nf | 8 +- subworkflows/nf-core/ensemblvep_annotate.nf | 25 +- subworkflows/nf-core/markduplicates.nf | 12 +- subworkflows/nf-core/recalibrate.nf | 17 +- subworkflows/nf-core/snpeff_annotate.nf | 25 +- subworkflows/nf-core/splitn_cigar_reads.nf | 10 +- workflows/rnavar.nf | 152 +++---- 19 files changed, 596 insertions(+), 534 deletions(-) create mode 100644 conf/modules.config.bk delete mode 100644 modules/nf-core/modules/star/align/meta.yml delete mode 100644 nf-core/modules/tabix/bgziptabix/main.nf delete mode 100644 nf-core/modules/tabix/bgziptabix/meta.yml diff --git a/.nf-core.yml b/.nf-core.yml index 4ac5d68f..c790afd7 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -6,5 +6,3 @@ lint: - assets/nf-core-rnavar_logo_light.png - docs/images/nf-core-rnavar_logo_light.png - docs/images/nf-core-rnavar_logo_dark.png - - lib/NfcoreTemplate.groovy - - lib/NfcoreSchema.groovy diff --git a/conf/modules.config b/conf/modules.config index d914c807..95059ec3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -1,168 +1,280 @@ /* ======================================================================================== - Config file for defining DSL2 per module options + Config file for defining DSL2 per module options and publishing paths ======================================================================================== Available keys to override module options: - args = Additional arguments appended to command in module. - args2 = Second set of arguments appended to command in module (multi-tool modules). - args3 = Third set of arguments appended to command in module (multi-tool modules). - publish_dir = Directory to publish results. - publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path - If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path - If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" - is appended as a directory to "publish_dir" path - If publish_by_meta = false / null - No directories are appended to "publish_dir" path - publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension - The value of "directory" is appended to the standard "publish_dir" path as defined above. - If publish_files = null (unspecified) - All files are published. - If publish_files = false - No files are published. - suffix = File name suffix for output files. + ext.args = Additional arguments appended to command in module. + ext.args2 = Second set of arguments appended to command in module (multi-tool modules). + ext.args3 = Third set of arguments appended to command in module (multi-tool modules). + ext.suffix = File name ext.suffix output files. Not available for nf-core modules + ext.prefix = File name prefix for output files. ---------------------------------------------------------------------------------------- */ -params { - modules { - 'cat_fastq' { - publish_dir = 'fastq' - } - 'star_genomegenerate' { - args = "--sjdbOverhang ${params.read_length - 1}" - publish_dir = 'genome/index' - } - 'star_align' { - args = "--outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" - publish_dir = "preprocessing" - publish_files = [:] - publish_by_meta = true - } - 'samtools_sort_genome' { - suffix = '.sorted' - publish_files = [:] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'samtools_merge_genome' { - suffix = '.merged' - publish_files = false - } - 'samtools_index_genome' { - args = '' - suffix = '.sorted' - publish_files = false - } - 'picard_markduplicates' { - args = 'ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT' - suffix = '.markdup.sorted' - publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'picard_markduplicates_samtools' { - args = '' - suffix = '.markdup.sorted' - publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'gatk_splitncigar_options' { - args = '' - suffix = '.split_cigar' - publish_files = false - } - 'gatk_baserecalibrator' { - args = '--use-original-qualities' - suffix = '' - publish_files = false - } - 'gatk_applybqsr' { - args = '--use-original-qualities --add-output-sam-program-record' - suffix = '.recal' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bam':'', 'bai':''] - } - 'samtools_index_recalibrate' { - suffix = '.recal' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bai':''] - } - 'samtools_stats_recalibrate' { - publish_by_meta = true - publish_dir = 'reports/samtools_stats' - } - 'gatk_haplotypecaller' { - args = '-dont-use-soft-clipped-bases' - suffix = '' - publish_files = false - } - 'gatk_intervallisttools' { - args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' - suffix = '' - publish_files = false - } - 'gatk_bedtointervallist' { - args = '' - publish_files = false - } - 'gatk_mergevcfs' { - suffix = '.haplotypecaller' - publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - 'gatk_indexfeaturefile' { - args = '' - suffix = '.haplotypecaller' - publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - 'gatk_variantfilter' { - args = '' - suffix = '.filtered' - publish_by_meta = true - publish_dir = 'variant_filtering' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - // ANNOTATE - 'snpeff' { - args = '-nodownload -canon -v' - publish_files = false - tag_base = '5.0' - } - 'bgziptabix_snpeff' { - publish_dir = 'variant_annotation' - suffix = '_snpEff.ann.vcf' - publish_by_meta = true - } - 'merge_vep' { - args = '--everything --filter_common --per_gene --total_length --offline' - publish_files = false - } - 'bgziptabix_merge_vep' { - publish_dir = 'variant_annotation' - suffix = '_snpEff_VEP.ann.vcf' - publish_by_meta = true - } - 'ensemblvep' { - args = '--everything --filter_common --per_gene --total_length --offline' - publish_files = false - tag_base = '104.3' - } - 'bgziptabix_vep' { - publish_dir = 'variant_annotation' - suffix = '_VEP.ann.vcf' - publish_by_meta = true +// GENERAL CONFIG + +process { + + publishDir = [ + path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + + withName: CUSTOM_DUMPSOFTWAREVERSIONS { + publishDir = [ + path: { "${params.outdir}/pipeline_info" }, + mode: params.publish_dir_mode, + pattern: '*_versions.yml' + ] + } +} + +// PREPARE FASTQ AND GENOME + +process { + withName: CAT_FASTQ { + publishDir = [ + path: { "${params.outdir}/fastq" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_merged_fastq + ] + } + withName: STAR_GENOMEGENERATE { + publishDir = [ + path: { "${params.outdir}/genome/index" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] + } + +} + +// ALIGNMENT +process { + // ALIGN_STAR + withName: '.*:ALIGN_STAR:STAR_ALIGN' { + ext.args = [ + '--outSAMtype BAM Unsorted', + '--readFilesCommand zcat', + params.save_unaligned ? '--outReadsUnmapped Fastx' : '', + params.read_length ? "--sjdbOverhang ${params.read_length - 1}" : '', + params.star_twopass ? '--twopassMode Basic' : '' + ].join(' ').trim() + publishDir = [ + [ + path: { "${params.outdir}/preprocessing/${meta.id}/log" }, + mode: params.publish_dir_mode, + pattern: '*.{out,tab}' + ], + [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: '*.bam', + enabled: params.save_align_intermeds + ], + [ + path: { "${params.outdir}/preprocessing/${meta.id}/unmapped" }, + mode: params.publish_dir_mode, + pattern: '*.fastq.gz', + enabled: params.save_unaligned + ] + ] + } +} + +// POST ALIGNMENT AND PREPROCESSING BAM + +process { + + withName: 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { + ext.suffix = '.sorted' + publishDir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.bam", + enabled: params.save_align_intermeds + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + ext.suffix = '.sorted' + publishDir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{bai,csi}", + enabled: params.save_align_intermeds + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_INTERVALLISTTOOLS' { + ext.args = [ + '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW' + '--UNIQUE true' + '--SORT true' + params.scatter_count ? "--SCATTER_COUNT $params.scatter_count" : '' + ].join(' ').trim() + } + withName: 'NFCORE_RNAVAR:RNAVAR:MARKDUPLICATES:PICARD_MARKDUPLICATES' { + ext.args = [ + 'ASSUME_SORTED=true', + 'VALIDATION_STRINGENCY=LENIENT', + params.remove_duplicates ? 'REMOVE_DUPLICATES=true' : '' + ].join(' ').trim() + ext.suffix = '.markdup.sorted' + publish_dir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.bam" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:MARKDUPLICATES:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + ext.suffix = '.markdup.sorted' + publish_dir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{bai,csi}" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_BASERECALIBRATOR' { + ext.args = '--use-original-qualities' + } + withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:APPLYBQSR' { + ext.args = [ + '--use-original-qualities', + '--add-output-sam-program-record' + ].join(' ').trim() + ext.suffix = '.recal' + publish_dir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{bam}" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + ext.suffix = '.recal' + publish_dir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{bai,csi}" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:SAMTOOLS_STATS' { + publish_dir = [ + path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{stats,flagstat}" + ] + } +} + +// VARIANT CALLING AND FILTERING + +process { + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_HAPLOTYPECALLER' { + ext.args = [ + '-dont-use-soft-clipped-bases' + params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '' + ].join(' ').trim() + } + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_MERGEVCFS' { + ext.suffix = '.haplotypecaller' + publish_dir = [ + path: { "${params.outdir}/variant_calling/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{vcf.gz}" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_INDEXFEATUREFILE' { + ext.suffix = '.haplotypecaller' + publish_dir = [ + path: { "${params.outdir}/variant_calling/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{vcf.gz.tbi}" + ] + } + withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_VARIANTFILTRATION' { + ext.suffix = '.filtered' + ext.args = [ + params.window ? "--window $params.window" : '' + params.cluster ? "--cluster $params.cluster" : '' + params.fs_filter ? "--filter-name \"FS\" --filter \"FS > $params.fs_filter\" " : '' + params.qd_filter ? "--filter-name \"QD\" --filter \"QD < $params.qd_filter\" " : '' + ].join(' ').trim() + publish_dir = [ + path: { "${params.outdir}/variant_calling/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{vcf.gz,vcf.gz.tbi}" + ] + } +} + +// VARIANT ANNOTATION + +process { + withName: 'ENSEMBLVEP' { + ext.args = '--everything --filter_common --per_gene --total_length --offline' + container = { "nfcore/vep:104.3.${params.genome}" } + publishDir = [ enabled: false ] + } + withName: 'SNPEFF' { + ext.args = '-nodownload -canon -v' + container = { "nfcore/snpeff:5.0.${params.genome}" } + publishDir = [ enabled: false ] + } + withName: 'ANNOTATION_BGZIPTABIX' { + publishDir = [ + path: { "${params.outdir}/variant_annotation/${meta.id}" }, + enabled: true, + pattern: "*{gz,gz.tbi}" + ] + } +} + +if(!params.skip_variantannotation && ('merge' in tools || 'snpeff' in tools)) { + process { + withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:SNPEFF_ANNOTATE:TABIX_BGZIPTABIX' { + ext.prefix = {"${meta.id}_snpEff.ann.vcf"} } - // QC modules - 'fastqc' { - args = "--quiet" - publish_files = false + } +} + +if(!params.skip_variantannotation && ('vep' in tools)) { + process { + withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:ENSEMBLVEP_ANNOTATE:TABIX_BGZIPTABIX' { + ext.prefix = {"${meta.id}_VEP.ann.vcf"} } - 'multiqc' { - args = "" - publish_dir = 'reports' + } +} + +if(!params.skip_variantannotation && ('merge' in tools) { + process { + withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:MERGE_ANNOTATE:TABIX_BGZIPTABIX' { + ext.prefix = {"${meta.id}_snpEff_VEP.ann.vcf"} } } + +} + +// QC + +process { + withName: MULTIQC { + ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + publishDir = [ + path: { "${params.outdir}/reports"}, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: FASTQC { + ext.args = '--quiet' + publishDir = [ + path: { "${params.outdir}/reports/fastqc/${meta.id}" } + enabled: false + ] + } } diff --git a/conf/modules.config.bk b/conf/modules.config.bk new file mode 100644 index 00000000..7b8efe54 --- /dev/null +++ b/conf/modules.config.bk @@ -0,0 +1,170 @@ +/* +======================================================================================== + Config file for defining DSL2 per module options +======================================================================================== + Available keys to override module options: + args = Additional arguments appended to command in module. + args2 = Second set of arguments appended to command in module (multi-tool modules). + args3 = Third set of arguments appended to command in module (multi-tool modules). + publish_dir = Directory to publish results. + publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path + If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path + If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" + is appended as a directory to "publish_dir" path + If publish_by_meta = false / null - No directories are appended to "publish_dir" path + publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + The value of "directory" is appended to the standard "publish_dir" path as defined above. + If publish_files = null (unspecified) - All files are published. + If publish_files = false - No files are published. + suffix = File name suffix for output files. +---------------------------------------------------------------------------------------- +*/ + +params { + modules { + 'cat_fastq' { + publish_dir = 'fastq' + } + 'star_genomegenerate' { + args = "--sjdbOverhang ${params.read_length - 1}" + publish_dir = 'genome/index' + } + 'star_align' { + args = "--outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" + publish_dir = "preprocessing" + publish_files = [:] + publish_by_meta = true + } + 'samtools_sort_genome' { + suffix = '.sorted' + publish_files = [:] + publish_dir = "preprocessing" + publish_by_meta = true + } + 'samtools_merge_genome' { + suffix = '.merged' + publish_files = false + } + 'samtools_index_genome' { + args = '' + suffix = '.sorted' + publish_files = false + } + 'picard_markduplicates' { + args = 'ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT' + suffix = '.markdup.sorted' + publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] + publish_dir = "preprocessing" + publish_by_meta = true + } + 'picard_markduplicates_samtools' { + args = '' + suffix = '.markdup.sorted' + publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] + publish_dir = "preprocessing" + publish_by_meta = true + } + 'gatk_splitncigar_options' { + args = '' + suffix = '.split_cigar' + publish_files = false + } + 'gatk_baserecalibrator' { + args = '--use-original-qualities' + suffix = '' + publish_files = false + } + 'gatk_applybqsr' { + args = '--use-original-qualities --add-output-sam-program-record' + suffix = '.recal' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['bam':'', 'bai':''] + } + 'samtools_index_recalibrate' { + suffix = '.recal' + publish_by_meta = true + publish_dir = 'preprocessing' + publish_files = ['bai':''] + } + + + 'samtools_stats_recalibrate' { + publish_by_meta = true + publish_dir = 'reports/samtools_stats' + } + 'gatk_haplotypecaller' { + args = '-dont-use-soft-clipped-bases' + suffix = '' + publish_files = false + } + 'gatk_intervallisttools' { + args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' + suffix = '' + publish_files = false + } + 'gatk_bedtointervallist' { + args = '' + publish_files = false + } + 'gatk_mergevcfs' { + suffix = '.haplotypecaller' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] + } + 'gatk_indexfeaturefile' { + args = '' + suffix = '.haplotypecaller' + publish_by_meta = true + publish_dir = 'variant_calling' + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] + } + 'gatk_variantfilter' { + args = '' + suffix = '.filtered' + publish_by_meta = true + publish_dir = 'variant_filtering' + publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] + } + // ANNOTATE + 'snpeff' { + args = '-nodownload -canon -v' + publish_files = false + tag_base = '5.0' + } + 'bgziptabix_snpeff' { + publish_dir = 'variant_annotation' + suffix = '_snpEff.ann.vcf' + publish_by_meta = true + } + 'merge_vep' { + args = '--everything --filter_common --per_gene --total_length --offline' + publish_files = false + } + 'bgziptabix_merge_vep' { + publish_dir = 'variant_annotation' + suffix = '_snpEff_VEP.ann.vcf' + publish_by_meta = true + } + 'ensemblvep' { + args = '--everything --filter_common --per_gene --total_length --offline' + publish_files = false + tag_base = '104.3' + } + 'bgziptabix_vep' { + publish_dir = 'variant_annotation' + suffix = '_VEP.ann.vcf' + publish_by_meta = true + } + // QC modules + 'fastqc' { + args = "--quiet" + publish_files = false + } + 'multiqc' { + args = "" + publish_dir = 'reports' + } + } +} diff --git a/modules.json b/modules.json index b8f829e5..2001e84d 100644 --- a/modules.json +++ b/modules.json @@ -77,16 +77,16 @@ }, "star/align": { "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" - }, + }, "star/genomegenerate": { "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, - "tabix/bgziptabix": { - "git_sha": "123" - }, + "tabix/bgziptabix": { + "git_sha": "123" + }, "untar": { "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml deleted file mode 100644 index 2d78b81a..00000000 --- a/modules/nf-core/modules/star/align/meta.yml +++ /dev/null @@ -1,81 +0,0 @@ -name: star_align -description: Align reads to a reference genome using STAR -keywords: - - align - - fasta - - genome - - reference -tools: - - star: - description: | - STAR is a software package for mapping DNA sequences against - a large reference genome, such as the human genome. - homepage: https://github.com/alexdobin/STAR - manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf - doi: 10.1093/bioinformatics/bts635 - licence: ['MIT'] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. - - index: - type: directory - description: STAR genome index - pattern: "star" -output: - - bam: - type: file - description: Output BAM file containing read alignments - pattern: "*.{bam}" - - log_final: - type: file - description: STAR final log file - pattern: "*Log.final.out" - - log_out: - type: file - description: STAR lot out file - pattern: "*Log.out" - - log_progress: - type: file - description: STAR log progress file - pattern: "*Log.progress.out" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - bam_sorted: - type: file - description: Sorted BAM file of read alignments (optional) - pattern: "*sortedByCoord.out.bam" - - bam_transcript: - type: file - description: Output BAM file of transcriptome alignment (optional) - pattern: "*toTranscriptome.out.bam" - - bam_unsorted: - type: file - description: Unsorted BAM file of read alignments (optional) - pattern: "*Aligned.unsort.out.bam" - - fastq: - type: file - description: Unmapped FastQ files (optional) - pattern: "*fastq.gz" - - tab: - type: file - description: STAR output tab file(s) (optional) - pattern: "*.tab" - - junction: - type: file - description: STAR chimeric junction output file (optional) - pattern: "*.out.junction" - -authors: - - "@kevinmenden" - - "@drpatelh" - - "@praveenraj2018" diff --git a/nf-core/modules/tabix/bgziptabix/main.nf b/nf-core/modules/tabix/bgziptabix/main.nf deleted file mode 100644 index e44a7226..00000000 --- a/nf-core/modules/tabix/bgziptabix/main.nf +++ /dev/null @@ -1,39 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process TABIX_BGZIPTABIX { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? 'bioconda::tabix=1.11' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0" - } else { - container "quay.io/biocontainers/tabix:1.11--hdfd78af_0" - } - - input: - tuple val(meta), path(input) - - output: - tuple val(meta), path("*.gz"), path("*.tbi"), emit: tbi - path "versions.yml" , emit: versions - - script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - """ - bgzip -c $options.args $input > ${prefix}.gz - tabix $options.args2 ${prefix}.gz - - cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/nf-core/modules/tabix/bgziptabix/meta.yml b/nf-core/modules/tabix/bgziptabix/meta.yml deleted file mode 100644 index f2aed84d..00000000 --- a/nf-core/modules/tabix/bgziptabix/meta.yml +++ /dev/null @@ -1,45 +0,0 @@ -name: tabix_bgziptabix -description: bgzip a sorted tab-delimited genome file and then create tabix index -keywords: - - bgzip - - compress - - index - - tabix - - vcf -tools: - - tabix: - description: Generic indexer for TAB-delimited genome position files. - homepage: https://www.htslib.org/doc/tabix.html - documentation: https://www.htslib.org/doc/tabix.1.html - doi: 10.1093/bioinformatics/btq671 - licence: ['MIT'] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - tab: - type: file - description: TAB-delimited genome position file - pattern: "*.{bed,gff,sam,vcf}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - gz: - type: file - description: Output compressed file - pattern: "*.{gz}" - - tbi: - type: file - description: tabix index file - pattern: "*.{gz.tbi}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@maxulysse" diff --git a/subworkflows/local/annotate.nf b/subworkflows/local/annotate.nf index 796fda54..47396545 100644 --- a/subworkflows/local/annotate.nf +++ b/subworkflows/local/annotate.nf @@ -2,36 +2,9 @@ // ANNOTATION // -params.annotation_cache = [:] -params.bgziptabix_merge_vep_options = [:] -params.bgziptabix_snpeff_options = [:] -params.bgziptabix_vep_options = [:] -params.merge_vep_options = [:] -params.snpeff_options = [:] -params.snpeff_tag = [:] -params.vep_options = [:] -params.vep_tag = [:] - -include { SNPEFF_ANNOTATE } from '../nf-core/snpeff_annotate' addParams( - bgziptabix_snpeff_options: params.bgziptabix_snpeff_options, - snpeff_options: params.snpeff_options, - snpeff_tag: params.snpeff_tag, - use_cache: params.annotation_cache -) - -include { ENSEMBLVEP_ANNOTATE as MERGE_ANNOTATE } from '../nf-core/ensemblvep_annotate' addParams( - bgziptabix_vep_options: params.bgziptabix_merge_vep_options, - use_cache: params.annotation_cache, - vep_options: params.merge_vep_options, - vep_tag: params.vep_tag -) - -include { ENSEMBLVEP_ANNOTATE } from '../nf-core/ensemblvep_annotate' addParams( - bgziptabix_vep_options: params.bgziptabix_vep_options, - use_cache: params.annotation_cache, - vep_options: params.vep_options, - vep_tag: params.vep_tag -) +include { SNPEFF_ANNOTATE } from '../nf-core/snpeff_annotate' +include { ENSEMBLVEP_ANNOTATE as MERGE_ANNOTATE } from '../nf-core/ensemblvep_annotate' +include { ENSEMBLVEP_ANNOTATE } from '../nf-core/ensemblvep_annotate' workflow ANNOTATE { take: diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 77b0e4b2..a4ab7c5d 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -2,23 +2,17 @@ // Uncompress and prepare reference genome files // -params.genome_options = [:] -params.gffread_options = [:] -params.index_options = [:] -params.star_index_options = [:] -params.star_untar_options = [:] - -include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' addParams(options: params.genome_options) -include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' addParams(options: params.genome_options) -include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' addParams(options: params.gffread_options) -include { GTF2BED } from '../../modules/local/gtf2bed' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' addParams(options: params.genome_options) -include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' addParams(options: params.genome_options) -include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' addParams(options: params.star_index_options) -include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' addParams(options: params.star_untar_options) +include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' //addParams(options: params.genome_options) +include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' //addParams(options: params.genome_options) +include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' //addParams(options: params.gffread_options) +include { GTF2BED } from '../../modules/local/gtf2bed' //addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) +include { GUNZIP as GUNZIP_GTF } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) +include { SAMTOOLS_FAIDX } from '../../modules/nf-core/modules/samtools/faidx/main' //addParams(options: params.genome_options) +include { STAR_GENOMEGENERATE } from '../../modules/nf-core/modules/star/genomegenerate/main' //addParams(options: params.star_index_options) +include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' //addParams(options: params.star_untar_options) workflow PREPARE_GENOME { diff --git a/subworkflows/local/splitncigar.nf b/subworkflows/local/splitncigar.nf index 6f0f480d..483e18b1 100644 --- a/subworkflows/local/splitncigar.nf +++ b/subworkflows/local/splitncigar.nf @@ -2,13 +2,13 @@ // PREPROCESSING OF ALIGNMENT FILE OVER THE INTERVALS IN PARALLEL // -params.gatk_splitncigar_options = [:] -params.samtools_index_options = [:] -params.samtools_merge_options = [:] +//params.gatk_splitncigar_options = [:] +//params.samtools_index_options = [:] +//params.samtools_merge_options = [:] -include { GATK4_SPLITNCIGAR } from '../nf-core/splitn_cigar_reads' addParams(gatk_splitncigar_options: params.gatk_splitncigar_options, samtools_index_options: params.samtools_index_options) -include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' addParams(options: params.samtools_merge_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) +include { GATK4_SPLITNCIGAR } from '../nf-core/splitn_cigar_reads' //addParams(gatk_splitncigar_options: params.gatk_splitncigar_options, samtools_index_options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' //addParams(options: params.samtools_merge_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) workflow SPLITNCIGAR { take: diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index fbe97292..1a8d7bab 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -2,20 +2,23 @@ // Alignment with STAR // -params.align_options = [:] -params.samtools_index_options = [:] -params.samtools_sort_options = [:] -params.samtools_stats_options = [:] -params.seq_platform = [:] +//params.align_options = [:] +//params.samtools_index_options = [:] +//params.samtools_sort_options = [:] +//params.samtools_stats_options = [:] +//params.seq_platform = [:] -include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' addParams( options: params.align_options, seq_platform: params.seq_platform) -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' //addParams( options: params.align_options, seq_platform: params.seq_platform) +include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' //addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) workflow ALIGN_STAR { take: - reads // channel: [ val(meta), [ reads ] ] - index // channel: /path/to/star/index/ - gtf // channel: /path/to/genome.gtf + reads // channel: [ val(meta), [ reads ] ] + index // channel: /path/to/star/index/ + gtf // channel: /path/to/genome.gtf + star_ignore_sjdbgtf // value: ignore gtf + seq_platform // value: sequencing platform + seq_center // value: sequencing centre main: @@ -24,7 +27,7 @@ workflow ALIGN_STAR { // // Map reads with STAR // - STAR_ALIGN ( reads, index, gtf ) + STAR_ALIGN ( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center ) ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first()) // diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index d52ec8ab..1746c834 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -2,13 +2,13 @@ // Sort, index BAM file and run samtools stats, flagstat and idxstats // -params.sort_options = [:] -params.index_options = [:] -params.stats_options = [:] +//params.sort_options = [:] +//params.index_options = [:] +//params.stats_options = [:] -include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' addParams( options: params.sort_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams( options: params.stats_options ) +include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' //addParams( options: params.sort_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.index_options ) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' //addParams( options: params.stats_options ) workflow BAM_SORT_SAMTOOLS { take: diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index b80482e3..34ab083c 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -2,11 +2,11 @@ // Run SAMtools stats, flagstat and idxstats // -params.options = [:] +//params.options = [:] -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams( options: params.options ) -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' addParams( options: params.options ) -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' addParams( options: params.options ) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' //addParams( options: params.options ) +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' //addParams( options: params.options ) +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' //addParams( options: params.options ) workflow BAM_STATS_SAMTOOLS { take: diff --git a/subworkflows/nf-core/ensemblvep_annotate.nf b/subworkflows/nf-core/ensemblvep_annotate.nf index b1b6725d..97aa3baf 100644 --- a/subworkflows/nf-core/ensemblvep_annotate.nf +++ b/subworkflows/nf-core/ensemblvep_annotate.nf @@ -2,18 +2,19 @@ // Run VEP to annotate VCF files // -params.bgziptabix_vep = [:] -params.vep_options = [:] -params.vep_tag = [:] -params.use_cache = [:] - -include { ENSEMBLVEP } from '../../modules/nf-core/modules/ensemblvep/main' addParams( - options: params.vep_options, - use_cache: params.use_cache, - vep_tag: params.vep_tag -) - -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' addParams(options: params.bgziptabix_vep_options) +//params.bgziptabix_vep = [:] +//params.vep_options = [:] +//params.vep_tag = [:] +//params.use_cache = [:] + +include { ENSEMBLVEP } from '../../modules/nf-core/modules/ensemblvep/main' +//addParams( +// options: params.vep_options, +// use_cache: params.use_cache, +// vep_tag: params.vep_tag +//) + +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' //addParams(options: params.bgziptabix_vep_options) workflow ENSEMBLVEP_ANNOTATE { take: diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index 05579425..482b1889 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -2,13 +2,13 @@ // Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats // -params.markduplicates_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] +//params.markduplicates_options = [:] +//params.samtools_index_options = [:] +//params.samtools_stats_options = [:] -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' addParams(options: params.samtools_stats_options) -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' addParams(options: params.markduplicates_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' //addParams(options: params.samtools_stats_options) +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' //addParams(options: params.markduplicates_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) workflow MARKDUPLICATES { take: diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index ec948135..7c6e4fe2 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -4,14 +4,14 @@ ======================================================================================== */ -params.applybqsr_options = [:] -params.qualimap_bamqc_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] +//params.applybqsr_options = [:] +//params.qualimap_bamqc_options = [:] +//params.samtools_index_options = [:] +//params.samtools_stats_options = [:] -include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' addParams(options: params.applybqsr_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams(options: params.samtools_index_options) -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' addParams(options: params.samtools_stats_options) +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' //addParams(options: params.applybqsr_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' //addParams(options: params.samtools_stats_options) workflow RECALIBRATE { take: @@ -21,7 +21,6 @@ workflow RECALIBRATE { dict // channel: [mandatory] dict fai // channel: [mandatory] fai fasta // channel: [mandatory] fasta - intervals // channel: [mandatory] intervals main: @@ -31,7 +30,7 @@ workflow RECALIBRATE { bam_recalibrated = Channel.empty() bam_reports = Channel.empty() - APPLYBQSR(bam, fasta, fai, dict, intervals) + APPLYBQSR(bam, fasta, fai, dict) bam_recalibrated = APPLYBQSR.out.bam ch_versions = ch_versions.mix(APPLYBQSR.out.versions.first()) diff --git a/subworkflows/nf-core/snpeff_annotate.nf b/subworkflows/nf-core/snpeff_annotate.nf index 4cef6187..238eb20b 100644 --- a/subworkflows/nf-core/snpeff_annotate.nf +++ b/subworkflows/nf-core/snpeff_annotate.nf @@ -2,18 +2,19 @@ // Run snpEff to annotate VCF files // -params.bgziptabix_snpeff = [:] -params.snpeff_options = [:] -params.snpeff_tag = [:] -params.use_cache = [:] - -include { SNPEFF } from '../../modules/nf-core/modules/snpeff/main' addParams( - options: params.snpeff_options, - snpeff_tag: params.snpeff_tag, - use_cache: params.use_cache -) - -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' addParams(options: params.bgziptabix_snpeff_options) +//params.bgziptabix_snpeff = [:] +//params.snpeff_options = [:] +//params.snpeff_tag = [:] +//params.use_cache = [:] + +include { SNPEFF } from '../../modules/nf-core/modules/snpeff/main' +//addParams( +// options: params.snpeff_options, +// snpeff_tag: params.snpeff_tag, +// use_cache: params.use_cache +//) + +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' //addParams(options: params.bgziptabix_snpeff_options) workflow SNPEFF_ANNOTATE { take: diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf index 59cba3db..4e63c322 100644 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -2,12 +2,12 @@ // GATK4 SplitNCigarReads // -params.gatk_splitncigar_options = [:] -params.samtools_index_options = [:] -params.samtools_stats_options = [:] +//params.gatk_splitncigar_options = [:] +//params.samtools_index_options = [:] +//params.samtools_stats_options = [:] -include { GATK4_SPLITNCIGARREADS } from '../../modules/local/gatk4/splitncigarreads/main' addParams( options: params.gatk_splitncigar_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' addParams( options: params.samtools_index_options ) +include { GATK4_SPLITNCIGARREADS } from '../../modules/local/gatk4/splitncigarreads/main' //addParams( options: params.gatk_splitncigar_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.samtools_index_options ) workflow GATK4_SPLITNCIGAR { take: diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index e2a18367..0844a3f7 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -48,12 +48,12 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi */ // Don't overwrite global params.modules, create a copy instead and use that within the main script. -def modules = params.modules.clone() +// def modules = params.modules.clone() // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +include { INPUT_CHECK } from '../subworkflows/local/input_check' //addParams( options: [:] ) /* ======================================================================================== @@ -61,15 +61,15 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( opti ======================================================================================== */ -def multiqc_options = modules['multiqc'] -multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' +//def multiqc_options = modules['multiqc'] +//multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' addParams( options: multiqc_options ) -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' addParams( options: [publish_files : ['_versions.yml':'']] ) +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' //addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' //addParams( options: multiqc_options ) +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' //addParams( options: [publish_files : ['_versions.yml':'']] ) /* ======================================================================================== @@ -81,39 +81,42 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/ def prepareToolIndices = [] prepareToolIndices = params.aligner -def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] -def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] -def untar_options = [publish_files: false] -def samtools_sort_genome_options = modules['samtools_sort_genome'] +//def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] +//def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] +//def untar_options = [publish_files: false] +//def samtools_sort_genome_options = modules['samtools_sort_genome'] -if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false +//if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false -modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +//modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -if (params.save_align_intermeds) { - samtools_sort_genome_options.publish_files.put('bam','') - samtools_sort_genome_options.publish_files.put('bai','') - samtools_sort_genome_options.publish_files.put('csi','') -} +//if (params.save_align_intermeds) { +// samtools_sort_genome_options.publish_files.put('bam','') +// samtools_sort_genome_options.publish_files.put('bai','') +// samtools_sort_genome_options.publish_files.put('csi','') +//} -if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false +//if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false // Additional paramaters to modules based on params -modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' -modules['star_align'].args += params.star_twopass ? Utils.joinModuleArgs(['--twopassMode Basic']) : '' -if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') -if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') +//modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' +//modules['star_align'].args += params.star_twopass ? Utils.joinModuleArgs(['--twopassMode Basic']) : '' +def seq_platform = params.seq_platform ? params.seq_platform : [] +def seq_center = params.seq_center ? params.seq_center : [] + +//if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') +//if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') -modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -modules['picard_markduplicates'].args += params.remove_duplicates ? Utils.joinModuleArgs(['REMOVE_DUPLICATES=true']) : '' -modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) +//modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' +//modules['picard_markduplicates'].args += params.remove_duplicates ? Utils.joinModuleArgs(['REMOVE_DUPLICATES=true']) : '' +//modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) -modules['gatk_haplotypecaller'].args += Utils.joinModuleArgs(["--standard-min-confidence-threshold-for-calling $params.stand_call_conf"]) +//modules['gatk_haplotypecaller'].args += Utils.joinModuleArgs(["--standard-min-confidence-threshold-for-calling $params.stand_call_conf"]) -if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) -if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) -if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) -if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) +//if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) +//if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) +//if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) +//if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) // Initialize varaint annotation associated channels def tools = params.annotate_tools ? params.annotate_tools.split(',').collect{it.trim().toLowerCase().replaceAll('-', '').replaceAll('_', '')} : [] @@ -125,64 +128,35 @@ def snpeff_cache = params.snpeff_cache ? params.snpeff_cache : [] def vep_cache = params.vep_cache ? params.vep_cache : [] // Include all the modules required for the pipeline -include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' addParams(options: modules['cat_fastq']) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' addParams(options: modules['gatk_baserecalibrator']) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' addParams(options: modules['gatk_bedtointervallist']) -include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' addParams(options: modules['gatk_haplotypecaller']) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' addParams(options: modules['gatk_intervallisttools']) -include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' addParams(options: modules['gatk_mergevcfs']) -include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' addParams(options: modules['gatk_indexfeaturefile']) -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' addParams(options: modules['gatk_variantfilter']) -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' addParams(options: modules['samtools_index_genome']) +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' //addParams(options: modules['cat_fastq']) +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' //addParams(options: modules['gatk_baserecalibrator']) +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' //addParams(options: modules['gatk_bedtointervallist']) +include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' //addParams(options: modules['gatk_haplotypecaller']) +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' //addParams(options: modules['gatk_intervallisttools']) +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' //addParams(options: modules['gatk_mergevcfs']) +include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' //addParams(options: modules['gatk_indexfeaturefile']) +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' //addParams(options: modules['gatk_variantfilter']) +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' //addParams(options: modules['samtools_index_genome']) // Include all the subworkflows required for the pipeline // Build the genome index and other reference files -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' addParams( - genome_options: publish_genome_options, - index_options: publish_index_options, - star_index_options: modules['star_genomegenerate'], - star_untar_options: untar_options -) +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' + // Align reads to genome and sort and index the alignment file -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' addParams( - align_options: modules['star_align'], - samtools_index_options: modules['samtools_index_genome'], - samtools_sort_options: modules['samtools_sort_genome'], - samtools_stats_options: modules['samtools_index_genome'], - seq_platform: params.seq_platform -) +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' + // Mark duplicates in the BAM file -include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' addParams( - markduplicates_options: modules['picard_markduplicates'], - samtools_index_options: modules['picard_markduplicates_samtools'], - samtools_stats_options: modules['picard_markduplicates_samtools'] -) +include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' + // Subworkflow - splits reads that contain Ns in their cigar string -include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' addParams( - gatk_splitncigar_options: modules['gatk_splitncigar_options'], - samtools_index_options: modules['samtools_index_genome'], - samtools_merge_options: modules['samtools_merge_genome'] -) +include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' // Estimate and correct systematic bias -include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' addParams( - applybqsr_options: modules['gatk_applybqsr'], - samtools_index_options: modules['samtools_index_recalibrate'], - samtools_stats_options: modules['samtools_stats_recalibrate'] -) +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' + // Annotate variants using snpEff or VEP or both -include { ANNOTATE } from '../subworkflows/local/annotate' addParams( - annotation_cache: params.annotation_cache, - bgziptabix_merge_vep_options: modules['bgziptabix_merge_vep'], - bgziptabix_snpeff_options: modules['bgziptabix_snpeff'], - bgziptabix_vep_options: modules['bgziptabix_vep'], - merge_vep_options: modules['merge_vep'], - snpeff_options: modules['snpeff'], - snpeff_tag: "${modules['snpeff'].tag_base}.${params.genome}", - vep_options: modules['ensemblvep'], - vep_tag: "${modules['ensemblvep'].tag_base}.${params.genome}" -) +include { ANNOTATE } from '../subworkflows/local/annotate' // Info required for completion email and summary def multiqc_report = [] @@ -270,7 +244,10 @@ workflow RNAVAR { ALIGN_STAR ( ch_cat_fastq, PREPARE_GENOME.out.star_index, - PREPARE_GENOME.out.gtf + PREPARE_GENOME.out.gtf, + params.star_ignore_sjdbgtf, + seq_platform, + seq_center ) ch_genome_bam = ALIGN_STAR.out.bam ch_genome_bam_index = ALIGN_STAR.out.bai @@ -303,15 +280,15 @@ workflow RNAVAR { ch_bqsr_table = Channel.empty() known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() - - ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() + //ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() + bam_splitncigar_interval = Channel.empty() + bam_splitncigar_interval = bam_splitncigar.join(ch_interval_list) GATK4_BASERECALIBRATOR( - bam_splitncigar, + bam_splitncigar_interval, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, - ch_interval_list_baserecalibrator, known_sites, known_sites_tbi ) @@ -322,8 +299,8 @@ workflow RNAVAR { bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() - - ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() + //ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() + bam_applybqsr.join(ch_interval_list) RECALIBRATE( ('bamqc' in params.skip_qc), @@ -331,8 +308,7 @@ workflow RNAVAR { bam_applybqsr, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.fasta, - ch_interval_list_applybqsr + PREPARE_GENOME.out.fasta ) bam_recalibrated = RECALIBRATE.out.bam From c0f6c4dcb5262b2395f04cd3aaca204023354704 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 4 Jan 2022 18:16:40 +0100 Subject: [PATCH 101/235] DSL2 update --- .github/workflows/awsfulltest.yml | 3 +- .nf-core.yml | 9 +-- lib/NfcoreSchema.groovy | 4 +- lib/NfcoreTemplate.groovy | 2 +- modules.json | 4 +- modules/nf-core/modules/star/align/meta.yml | 81 +++++++++++++++++++++ 6 files changed, 88 insertions(+), 15 deletions(-) create mode 100644 modules/nf-core/modules/star/align/meta.yml diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 2c8491bc..abed9a49 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -15,10 +15,9 @@ jobs: steps: - name: Launch workflow via tower uses: nf-core/tower-action@master - # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters - + with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} bearer_token: ${{ secrets.TOWER_BEARER_TOKEN }} diff --git a/.nf-core.yml b/.nf-core.yml index c790afd7..8b137891 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,8 +1 @@ -lint: - files_unchanged: - - .github/workflows/linting.yml - - .github/CONTRIBUTING.md - - assets/multiqc_config.yaml - - assets/nf-core-rnavar_logo_light.png - - docs/images/nf-core-rnavar_logo_light.png - - docs/images/nf-core-rnavar_logo_dark.png + diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 5f681078..40ab65f2 100755 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -206,7 +206,7 @@ class NfcoreSchema { } def type = '[' + group_params.get(param).type + ']' def description = group_params.get(param).description - def defaultValue = group_params.get(param).default ? " [default: " + group_params.get(param).default.toString() + "]" : '' + def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' def description_default = description + colors.dim + defaultValue + colors.reset // Wrap long description texts // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap @@ -362,7 +362,7 @@ class NfcoreSchema { } } for (ex in causingExceptions) { - printExceptions(ex, params_json, log) + printExceptions(ex, params_json, log, enums) } } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 06499409..2fc0a9b9 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -24,7 +24,7 @@ class NfcoreTemplate { public static void checkConfigProvided(workflow, log) { if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute enviroment but can be acheived via one or more of the following:\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + diff --git a/modules.json b/modules.json index 2001e84d..346d2681 100644 --- a/modules.json +++ b/modules.json @@ -76,13 +76,13 @@ "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" }, "star/align": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" }, "star/genomegenerate": { "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "tabix/bgziptabix": { - "git_sha": "123" + "git_sha": "e22966ce74340cb671576143e5fdbbd71670cffa" }, "untar": { "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" diff --git a/modules/nf-core/modules/star/align/meta.yml b/modules/nf-core/modules/star/align/meta.yml new file mode 100644 index 00000000..2d78b81a --- /dev/null +++ b/modules/nf-core/modules/star/align/meta.yml @@ -0,0 +1,81 @@ +name: star_align +description: Align reads to a reference genome using STAR +keywords: + - align + - fasta + - genome + - reference +tools: + - star: + description: | + STAR is a software package for mapping DNA sequences against + a large reference genome, such as the human genome. + homepage: https://github.com/alexdobin/STAR + manual: https://github.com/alexdobin/STAR/blob/master/doc/STARmanual.pdf + doi: 10.1093/bioinformatics/bts635 + licence: ['MIT'] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - index: + type: directory + description: STAR genome index + pattern: "star" +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - log_final: + type: file + description: STAR final log file + pattern: "*Log.final.out" + - log_out: + type: file + description: STAR lot out file + pattern: "*Log.out" + - log_progress: + type: file + description: STAR log progress file + pattern: "*Log.progress.out" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam_sorted: + type: file + description: Sorted BAM file of read alignments (optional) + pattern: "*sortedByCoord.out.bam" + - bam_transcript: + type: file + description: Output BAM file of transcriptome alignment (optional) + pattern: "*toTranscriptome.out.bam" + - bam_unsorted: + type: file + description: Unsorted BAM file of read alignments (optional) + pattern: "*Aligned.unsort.out.bam" + - fastq: + type: file + description: Unmapped FastQ files (optional) + pattern: "*fastq.gz" + - tab: + type: file + description: STAR output tab file(s) (optional) + pattern: "*.tab" + - junction: + type: file + description: STAR chimeric junction output file (optional) + pattern: "*.out.junction" + +authors: + - "@kevinmenden" + - "@drpatelh" + - "@praveenraj2018" From 9288f41e22e833fc82178227dc5a4590c186ca40 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 4 Jan 2022 18:17:26 +0100 Subject: [PATCH 102/235] Fix: lint errors --- .github/CONTRIBUTING.md | 38 +++++---------------------- .github/workflows/linting.yml | 2 +- assets/nf-core-rnavar_logo_light.png | Bin 65097 -> 9910 bytes 3 files changed, 8 insertions(+), 32 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 7b8f6f63..cb0a4bf1 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -68,16 +68,13 @@ If you wish to contribute a new step, please use the following coding standards: 1. Define the corresponding input channel into your new process from the expected previous process channel 2. Write the process block (see below). 3. Define the output channel if needed (see below). -4. Add any new flags/options to `nextflow.config` with a default (see below). -5. Add any new flags/options to `nextflow_schema.json` with help text (with `nf-core schema build`). -6. Add any new flags/options to the help message (for integer/text parameters, print to help the corresponding `nextflow.config` parameter). -7. Add sanity checks for all relevant parameters. -8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. -9. Do local tests that the new code works properly and as expected. -10. Add a new test command in `.github/workflow/ci.yml`. -11. If applicable add a [MultiQC](https://https://multiqc.info/) module. -12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order. -13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`. +4. Add any new parameters to `nextflow.config` with a default (see below). +5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool). +6. Add sanity checks and validation for all relevant parameters. +7. Perform local tests to validate that the new code works as expected. +8. If applicable, add a new test command in `.github/workflow/ci.yml`. +9. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, file name clean up and module plots are in the appropriate order. If applicable, add a [MultiQC](https://https://multiqc.info/) module. +10. Add a description of the output files and if relevant any appropriate images from the MultiQC report to `docs/output.md`. ### Default values @@ -102,27 +99,6 @@ Please use the following naming schemes, to make it easy to understand what is g If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` -### Software version reporting - -If you add a new tool to the pipeline, please ensure you add the information of the tool to the `get_software_version` process. - -Add to the script block of the process, something like the following: - -```bash - --version &> v_.txt 2>&1 || true -``` - -or - -```bash - --help | head -n 1 &> v_.txt 2>&1 || true -``` - -You then need to edit the script `bin/scrape_software_versions.py` to: - -1. Add a Python regex for your tool's `--version` output (as in stored in the `v_.txt` file), to ensure the version is reported as a `v` and the version number e.g. `v2.1.1` -2. Add a HTML entry to the `OrderedDict` for formatting in MultiQC. - ### Images and figures For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines). diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 3c9ccf17..3b448773 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -120,7 +120,7 @@ jobs: - name: Install dependencies run: | python -m pip install --upgrade pip - pip install --upgrade --force-reinstall git+https://github.com/nf-core/tools.git@dev + pip install nf-core - name: Run nf-core lint env: diff --git a/assets/nf-core-rnavar_logo_light.png b/assets/nf-core-rnavar_logo_light.png index 509f923f092c056161198ce9745d0fe4fc83e867..9cca37e801c2dad85523ea3419b9aa15f0468640 100644 GIT binary patch literal 9910 zcmbulXIN8B)HR$CAaq0!q)JyHpfpi>??ocL2uMJBuhIgDii(C#=qg2e?+6hQLRVTq zNM(;MAHauTF$(FngEo$8uCTu_7 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z9IFh0(_9i(ST`-?iCaXzN!0$r?Q|N-52-XXWKHV|IcC5C>#C~gADNkvb}iLwGIhJv zBQ!a%&sFxGcz6bHEaORt>hz5}oKUH7ceGK9Pg(#23UU#KR}}R{GKsO6f=hP6a0jCB ziPmy~twPARd#Y)j5!4WVazE22>*8qPbS{-87I`OTA_lETTvA< From eb066f6200f3ba575f0672cf6459298c593c303c Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 14 Jan 2022 06:41:45 +0100 Subject: [PATCH 103/235] DSL2 transition; Fix: Issue with multiple samples --- .github/workflows/ci.yml | 1 + conf/modules.config | 219 ++++++++++++------ conf/modules.config.bk | 170 -------------- modules.json | 32 +-- .../nf-core/modules/gatk4/applybqsr/main.nf | 6 +- .../modules/gatk4/baserecalibrator/main.nf | 6 +- .../modules/gatk4/bedtointervallist/main.nf | 6 +- .../gatk4/createsequencedictionary/main.nf | 6 +- .../modules/gatk4/indexfeaturefile/main.nf | 6 +- .../modules/gatk4/intervallisttools/main.nf | 6 +- .../nf-core/modules/gatk4/mergevcfs/main.nf | 6 +- .../modules/gatk4/variantfiltration/main.nf | 6 +- .../modules/picard/markduplicates/main.nf | 6 +- .../nf-core/modules/samtools/faidx/main.nf | 9 +- .../nf-core/modules/samtools/flagstat/main.nf | 7 +- .../nf-core/modules/samtools/idxstats/main.nf | 6 +- .../nf-core/modules/samtools/index/main.nf | 6 +- .../nf-core/modules/samtools/merge/main.nf | 10 +- modules/nf-core/modules/samtools/sort/main.nf | 1 + .../nf-core/modules/samtools/stats/main.nf | 7 +- subworkflows/local/annotate.nf | 45 ++-- subworkflows/local/prepare_genome.nf | 29 +-- subworkflows/local/splitncigar.nf | 12 +- subworkflows/nf-core/ensemblvep_annotate.nf | 6 +- subworkflows/nf-core/snpeff_annotate.nf | 6 +- tests/csv/samplesheet_test.csv | 1 + tests/test_default.yml | 4 +- tests/test_defaultmultiplesamples.yml | 19 ++ tests/test_removeduplicates.yml | 4 +- workflows/rnavar.nf | 54 +++-- 30 files changed, 330 insertions(+), 372 deletions(-) delete mode 100644 conf/modules.config.bk create mode 100644 tests/test_defaultmultiplesamples.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 63d19123..44e10c1b 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,6 +28,7 @@ jobs: NXF_EDGE: '1' test: - 'default' + - 'defaultmultiplesamples' - 'annotation' - 'removeduplicates' steps: diff --git a/conf/modules.config b/conf/modules.config index 95059ec3..3a8a38a5 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,6 +21,14 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] + withName: 'NFCORE_RNAVAR:RNAVAR:INPUT_CHECK:SAMPLESHEET_CHECK' { + publishDir = [ + path: { "${params.outdir}/pipeline_info" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, @@ -30,9 +38,10 @@ process { } } -// PREPARE FASTQ AND GENOME +// PREPARE GENOME process { + withName: CAT_FASTQ { publishDir = [ path: { "${params.outdir}/fastq" }, @@ -41,7 +50,25 @@ process { enabled: params.save_merged_fastq ] } + + withName: 'GUNZIP_.*' { + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: 'copy', + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] + } + + withName: 'UNTAR_.*' { + ext.args2 = '--no-same-owner' + } + withName: STAR_GENOMEGENERATE { + ext.args = params.read_length ? "--sjdbOverhang ${params.read_length - 1}" : '' + } + + withName: 'UNTAR_.*|STAR_GENOMEGENERATE' { publishDir = [ path: { "${params.outdir}/genome/index" }, mode: params.publish_dir_mode, @@ -50,12 +77,62 @@ process { ] } + withName: GTF2BED { + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] + } + + withName: GET_CHROM_SIZES { + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] + } + + withName: GATK4_CREATESEQUENCEDICTIONARY { + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] + } +} + +// READ QC AND QC REPORT + +process { + + withName: MULTIQC { + ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + publishDir = [ + path: { "${params.outdir}/reports"}, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + + withName: FASTQC { + ext.args = '--quiet' + publishDir = [ + path: { "${params.outdir}/reports/fastqc/${meta.id}" }, + enabled: false + ] + } } // ALIGNMENT process { + // ALIGN_STAR - withName: '.*:ALIGN_STAR:STAR_ALIGN' { + + withName: STAR_ALIGN { ext.args = [ '--outSAMtype BAM Unsorted', '--readFilesCommand zcat', @@ -89,7 +166,7 @@ process { process { - withName: 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { + withName: '.*:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { ext.suffix = '.sorted' publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, @@ -98,7 +175,8 @@ process { enabled: params.save_align_intermeds ] } - withName: 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { + + withName: '.*:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' ext.suffix = '.sorted' publishDir = [ @@ -108,64 +186,86 @@ process { enabled: params.save_align_intermeds ] } - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_INTERVALLISTTOOLS' { + + withName: GATK4_BEDTOINTERVALLIST { + publishDir = [ enabled: false ] + } + + withName: GATK4_INTERVALLISTTOOLS { ext.args = [ - '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW' - '--UNIQUE true' - '--SORT true' + '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW', + '--UNIQUE true', + '--SORT true', params.scatter_count ? "--SCATTER_COUNT $params.scatter_count" : '' ].join(' ').trim() + publishDir = [ enabled: false ] } - withName: 'NFCORE_RNAVAR:RNAVAR:MARKDUPLICATES:PICARD_MARKDUPLICATES' { + + withName: '.*:MARKDUPLICATES:PICARD_MARKDUPLICATES' { ext.args = [ 'ASSUME_SORTED=true', 'VALIDATION_STRINGENCY=LENIENT', params.remove_duplicates ? 'REMOVE_DUPLICATES=true' : '' ].join(' ').trim() - ext.suffix = '.markdup.sorted' - publish_dir = [ + ext.prefix = {"${meta.id}.markdup.sorted"} + publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, - pattern: "*.bam" + enabled: true, + pattern: "*.{bam}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:MARKDUPLICATES:SAMTOOLS_INDEX' { + + withName: '.*:MARKDUPLICATES:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' - ext.suffix = '.markdup.sorted' - publish_dir = [ + ext.prefix = {"${meta.id}.markdup.sorted"} + publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, + enabled: true, pattern: "*.{bai,csi}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_BASERECALIBRATOR' { + + withName: '.*:SPLITNCIGAR:.*' { + publishDir = [ enabled: false ] + } + + withName: GATK4_BASERECALIBRATOR { ext.args = '--use-original-qualities' + publishDir = [ enabled: false ] } - withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:APPLYBQSR' { + + withName: '.*:RECALIBRATE:APPLYBQSR' { ext.args = [ '--use-original-qualities', '--add-output-sam-program-record' ].join(' ').trim() - ext.suffix = '.recal' - publish_dir = [ + ext.prefix = {"${meta.id}.recal"} + publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, + enabled: true, pattern: "*.{bam}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:SAMTOOLS_INDEX' { + + withName: '.*:RECALIBRATE:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' - ext.suffix = '.recal' - publish_dir = [ + ext.prefix = {"${meta.id}.recal"} + publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, + enabled: true, pattern: "*.{bai,csi}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:RECALIBRATE:SAMTOOLS_STATS' { - publish_dir = [ + + withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { + publishDir = [ path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, mode: params.publish_dir_mode, + enabled: true, pattern: "*.{stats,flagstat}" ] } @@ -174,37 +274,42 @@ process { // VARIANT CALLING AND FILTERING process { - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_HAPLOTYPECALLER' { + + withName: GATK4_HAPLOTYPECALLER { ext.args = [ - '-dont-use-soft-clipped-bases' + '-dont-use-soft-clipped-bases', params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '' ].join(' ').trim() + publishDir = [ enabled: false ] } - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_MERGEVCFS' { - ext.suffix = '.haplotypecaller' - publish_dir = [ + + withName: GATK4_MERGEVCFS { + ext.prefix = {"${meta.id}.haplotypecaller"} + publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, pattern: "*.{vcf.gz}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_INDEXFEATUREFILE' { - ext.suffix = '.haplotypecaller' - publish_dir = [ + + withName: GATK4_INDEXFEATUREFILE { + ext.prefix = {"${meta.id}.haplotypecaller"} + publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, pattern: "*.{vcf.gz.tbi}" ] } - withName: 'NFCORE_RNAVAR:RNAVAR:GATK4_VARIANTFILTRATION' { - ext.suffix = '.filtered' + + withName: GATK4_VARIANTFILTRATION { + ext.prefix = {"${meta.id}.haplotypecaller.filtered"} ext.args = [ - params.window ? "--window $params.window" : '' - params.cluster ? "--cluster $params.cluster" : '' - params.fs_filter ? "--filter-name \"FS\" --filter \"FS > $params.fs_filter\" " : '' - params.qd_filter ? "--filter-name \"QD\" --filter \"QD < $params.qd_filter\" " : '' + params.window ? "--window $params.window" : '', + params.cluster ? "--cluster $params.cluster" : '', + params.fs_filter ? "--filter-name \"FS\" --filter \"FS > $params.fs_filter\" " : '', + params.qd_filter ? "--filter-name \"QD\" --filter \"QD < $params.qd_filter\" " : '', ].join(' ').trim() - publish_dir = [ + publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, pattern: "*.{vcf.gz,vcf.gz.tbi}" @@ -215,26 +320,29 @@ process { // VARIANT ANNOTATION process { + withName: 'ENSEMBLVEP' { ext.args = '--everything --filter_common --per_gene --total_length --offline' container = { "nfcore/vep:104.3.${params.genome}" } publishDir = [ enabled: false ] } + withName: 'SNPEFF' { ext.args = '-nodownload -canon -v' container = { "nfcore/snpeff:5.0.${params.genome}" } publishDir = [ enabled: false ] } - withName: 'ANNOTATION_BGZIPTABIX' { - publishDir = [ + + withName: 'TABIX_BGZIPTABIX' { + publishDir = [ path: { "${params.outdir}/variant_annotation/${meta.id}" }, enabled: true, - pattern: "*{gz,gz.tbi}" + pattern: "*.{gz,gz.tbi}" ] } } -if(!params.skip_variantannotation && ('merge' in tools || 'snpeff' in tools)) { +if( (params.annotate_tools) && (params.annotate_tools.contains('snpeff') || params.annotate_tools.contains('merge')) ) { process { withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:SNPEFF_ANNOTATE:TABIX_BGZIPTABIX' { ext.prefix = {"${meta.id}_snpEff.ann.vcf"} @@ -242,7 +350,7 @@ if(!params.skip_variantannotation && ('merge' in tools || 'snpeff' in tools)) { } } -if(!params.skip_variantannotation && ('vep' in tools)) { +if( (params.annotate_tools) && (params.annotate_tools.contains('vep')) ) { process { withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:ENSEMBLVEP_ANNOTATE:TABIX_BGZIPTABIX' { ext.prefix = {"${meta.id}_VEP.ann.vcf"} @@ -250,31 +358,10 @@ if(!params.skip_variantannotation && ('vep' in tools)) { } } -if(!params.skip_variantannotation && ('merge' in tools) { +if( (params.annotate_tools) && (params.annotate_tools.contains('merge')) ) { process { withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:MERGE_ANNOTATE:TABIX_BGZIPTABIX' { ext.prefix = {"${meta.id}_snpEff_VEP.ann.vcf"} } } - -} - -// QC - -process { - withName: MULTIQC { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' - publishDir = [ - path: { "${params.outdir}/reports"}, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { - ext.args = '--quiet' - publishDir = [ - path: { "${params.outdir}/reports/fastqc/${meta.id}" } - enabled: false - ] - } } diff --git a/conf/modules.config.bk b/conf/modules.config.bk deleted file mode 100644 index 7b8efe54..00000000 --- a/conf/modules.config.bk +++ /dev/null @@ -1,170 +0,0 @@ -/* -======================================================================================== - Config file for defining DSL2 per module options -======================================================================================== - Available keys to override module options: - args = Additional arguments appended to command in module. - args2 = Second set of arguments appended to command in module (multi-tool modules). - args3 = Third set of arguments appended to command in module (multi-tool modules). - publish_dir = Directory to publish results. - publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path - If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path - If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" - is appended as a directory to "publish_dir" path - If publish_by_meta = false / null - No directories are appended to "publish_dir" path - publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension - The value of "directory" is appended to the standard "publish_dir" path as defined above. - If publish_files = null (unspecified) - All files are published. - If publish_files = false - No files are published. - suffix = File name suffix for output files. ----------------------------------------------------------------------------------------- -*/ - -params { - modules { - 'cat_fastq' { - publish_dir = 'fastq' - } - 'star_genomegenerate' { - args = "--sjdbOverhang ${params.read_length - 1}" - publish_dir = 'genome/index' - } - 'star_align' { - args = "--outSAMtype BAM Unsorted --readFilesCommand zcat --sjdbOverhang ${params.read_length - 1}" - publish_dir = "preprocessing" - publish_files = [:] - publish_by_meta = true - } - 'samtools_sort_genome' { - suffix = '.sorted' - publish_files = [:] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'samtools_merge_genome' { - suffix = '.merged' - publish_files = false - } - 'samtools_index_genome' { - args = '' - suffix = '.sorted' - publish_files = false - } - 'picard_markduplicates' { - args = 'ASSUME_SORTED=true VALIDATION_STRINGENCY=LENIENT' - suffix = '.markdup.sorted' - publish_files = ['bam': '', 'metrics.txt':'picard_metrics'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'picard_markduplicates_samtools' { - args = '' - suffix = '.markdup.sorted' - publish_files = ['bai':'', 'csi':'', 'stats':'samtools_stats', 'flagstat':'samtools_stats', 'idxstats':'samtools_stats'] - publish_dir = "preprocessing" - publish_by_meta = true - } - 'gatk_splitncigar_options' { - args = '' - suffix = '.split_cigar' - publish_files = false - } - 'gatk_baserecalibrator' { - args = '--use-original-qualities' - suffix = '' - publish_files = false - } - 'gatk_applybqsr' { - args = '--use-original-qualities --add-output-sam-program-record' - suffix = '.recal' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bam':'', 'bai':''] - } - 'samtools_index_recalibrate' { - suffix = '.recal' - publish_by_meta = true - publish_dir = 'preprocessing' - publish_files = ['bai':''] - } - - - 'samtools_stats_recalibrate' { - publish_by_meta = true - publish_dir = 'reports/samtools_stats' - } - 'gatk_haplotypecaller' { - args = '-dont-use-soft-clipped-bases' - suffix = '' - publish_files = false - } - 'gatk_intervallisttools' { - args = '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW --UNIQUE true --SORT true' - suffix = '' - publish_files = false - } - 'gatk_bedtointervallist' { - args = '' - publish_files = false - } - 'gatk_mergevcfs' { - suffix = '.haplotypecaller' - publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - 'gatk_indexfeaturefile' { - args = '' - suffix = '.haplotypecaller' - publish_by_meta = true - publish_dir = 'variant_calling' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - 'gatk_variantfilter' { - args = '' - suffix = '.filtered' - publish_by_meta = true - publish_dir = 'variant_filtering' - publish_files = ['vcf.gz':'', 'vcf.gz.tbi':''] - } - // ANNOTATE - 'snpeff' { - args = '-nodownload -canon -v' - publish_files = false - tag_base = '5.0' - } - 'bgziptabix_snpeff' { - publish_dir = 'variant_annotation' - suffix = '_snpEff.ann.vcf' - publish_by_meta = true - } - 'merge_vep' { - args = '--everything --filter_common --per_gene --total_length --offline' - publish_files = false - } - 'bgziptabix_merge_vep' { - publish_dir = 'variant_annotation' - suffix = '_snpEff_VEP.ann.vcf' - publish_by_meta = true - } - 'ensemblvep' { - args = '--everything --filter_common --per_gene --total_length --offline' - publish_files = false - tag_base = '104.3' - } - 'bgziptabix_vep' { - publish_dir = 'variant_annotation' - suffix = '_VEP.ann.vcf' - publish_by_meta = true - } - // QC modules - 'fastqc' { - args = "--quiet" - publish_files = false - } - 'multiqc' { - args = "" - publish_dir = 'reports' - } - } -} diff --git a/modules.json b/modules.json index 346d2681..44e00ef7 100644 --- a/modules.json +++ b/modules.json @@ -16,28 +16,28 @@ "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" }, "gatk4/applybqsr": { - "git_sha": "a6e0629e2430a9d4061ddc8afdf4cff8c93080b5" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/baserecalibrator": { - "git_sha": "a6e0629e2430a9d4061ddc8afdf4cff8c93080b5" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/bedtointervallist": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/createsequencedictionary": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/indexfeaturefile": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/intervallisttools": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/mergevcfs": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gatk4/variantfiltration": { - "git_sha": "50eafb19c689bca7747a6d44b8e7a555ba172f4b" + "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" }, "gffread": { "git_sha": "d473a247d2e0c619b0df877ea19d9a5a98c8e3c8" @@ -49,28 +49,28 @@ "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "picard/markduplicates": { - "git_sha": "0d1e21686a586447b7592e40da9b3a7cdeedf03c" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/faidx": { - "git_sha": "e0aa89141ffecb5f54d230f7ea46de242b74e084" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/flagstat": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/idxstats": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/index": { - "git_sha": "0fafaeebf52cc5ab554b83297ed02a48d852a848" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/merge": { - "git_sha": "d473a247d2e0c619b0df877ea19d9a5a98c8e3c8" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/sort": { - "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "samtools/stats": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" }, "snpeff": { "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index 22702211..c246190a 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -2,10 +2,10 @@ process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals) diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index 3a26d6cc..42391d62 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -2,10 +2,10 @@ process GATK4_BASERECALIBRATOR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index 76d0f8a4..37f46f48 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -2,10 +2,10 @@ process GATK4_BEDTOINTERVALLIST { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(bed) diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index 4d82a643..2cbd8948 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -2,10 +2,10 @@ process GATK4_CREATESEQUENCEDICTIONARY { tag "$fasta" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: path fasta diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index 63633e5f..ecd24173 100644 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -2,10 +2,10 @@ process GATK4_INDEXFEATUREFILE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(feature_file) diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index b1881932..17412160 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -2,10 +2,10 @@ process GATK4_INTERVALLISTTOOLS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(interval_list) diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf index 9158a242..b2e584a8 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -2,10 +2,10 @@ process GATK4_MERGEVCFS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(vcfs) diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index 32e4aae1..beaec0c0 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -2,10 +2,10 @@ process GATK4_VARIANTFILTRATION { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" input: tuple val(meta), path(vcf), path(vcf_tbi) diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index 3087bff4..7990d7e6 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -2,10 +2,10 @@ process PICARD_MARKDUPLICATES { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::picard=2.26.7" : null) + conda (params.enable_conda ? "bioconda::picard=2.26.10" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:2.26.7--hdfd78af_0' : - 'quay.io/biocontainers/picard:2.26.7--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' : + 'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index d8308b03..58c445d8 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -11,13 +11,16 @@ process SAMTOOLS_FAIDX { tuple val(meta), path(fasta) output: - tuple val(meta), path ("*.fai") , emit: fai - path "versions.yml" , emit: versions + tuple val(meta), path ("*.fai"), emit: fai + path "versions.yml" , emit: versions script: def args = task.ext.args ?: '' """ - samtools faidx $fasta + samtools \\ + faidx \\ + $fasta + cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index 03721d0b..119adf77 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -17,7 +17,12 @@ process SAMTOOLS_FLAGSTAT { script: def args = task.ext.args ?: '' """ - samtools flagstat --threads ${task.cpus-1} $bam > ${bam}.flagstat + samtools \\ + flagstat \\ + --threads ${task.cpus-1} \\ + $bam \\ + > ${bam}.flagstat + cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index cd068679..fc54e676 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -17,7 +17,11 @@ process SAMTOOLS_IDXSTATS { script: def args = task.ext.args ?: '' """ - samtools idxstats $bam > ${bam}.idxstats + samtools \\ + idxstats \\ + $bam \\ + > ${bam}.idxstats + cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index db025a8f..c4fa2c63 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -19,7 +19,11 @@ process SAMTOOLS_INDEX { script: def args = task.ext.args ?: '' """ - samtools index -@ ${task.cpus-1} $args $input + samtools \\ + index \\ + -@ ${task.cpus-1} \\ + $args \\ + $input cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index fcfcf61f..a318bfc4 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -12,7 +12,7 @@ process SAMTOOLS_MERGE { path fasta output: - tuple val(meta), path("${prefix}.bam"), optional:true, emit: bam + tuple val(meta), path("${prefix}.bam") , optional:true, emit: bam tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram path "versions.yml" , emit: versions @@ -22,7 +22,13 @@ process SAMTOOLS_MERGE { def file_type = input_files[0].getExtension() def reference = fasta ? "--reference ${fasta}" : "" """ - samtools merge --threads ${task.cpus-1} $args ${reference} ${prefix}.${file_type} $input_files + samtools \\ + merge \\ + --threads ${task.cpus-1} \\ + $args \\ + ${reference} \\ + ${prefix}.${file_type} \\ + $input_files cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index 0c2cf25e..42c7bbf4 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -17,6 +17,7 @@ process SAMTOOLS_SORT { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ samtools sort $args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index 83c87002..7209070d 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -19,7 +19,12 @@ process SAMTOOLS_STATS { def args = task.ext.args ?: '' def reference = fasta ? "--reference ${fasta}" : "" """ - samtools stats --threads ${task.cpus-1} ${reference} ${input} > ${input}.stats + samtools \\ + stats \\ + --threads ${task.cpus-1} \\ + ${reference} \\ + ${input} \\ + > ${input}.stats cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/annotate.nf b/subworkflows/local/annotate.nf index 47396545..4c7b03ab 100644 --- a/subworkflows/local/annotate.nf +++ b/subworkflows/local/annotate.nf @@ -19,39 +19,34 @@ workflow ANNOTATE { main: - ch_versions = Channel.empty() - merge_vcf_ann = Channel.empty() - merge_vep_report = Channel.empty() - merge_vep_version = Channel.empty() - snpeff_report = Channel.empty() - snpeff_vcf_ann = Channel.empty() - snpeff_version = Channel.empty() - vep_report = Channel.empty() - vep_vcf_ann = Channel.empty() - vep_version = Channel.empty() - - if ('snpeff' in tools || 'merge' in tools) { - (snpeff_vcf_ann, snpeff_report, snpeff_version) = SNPEFF_ANNOTATE(vcf, snpeff_db, snpeff_cache) + ch_versions = Channel.empty() + ch_reports = Channel.empty() + ch_vcf_ann = Channel.empty() + + if (tools.contains('snpeff') || tools.contains('merge')) { + SNPEFF_ANNOTATE(vcf, snpeff_db, snpeff_cache) + ch_vcf_ann = ch_vcf_ann.mix(SNPEFF_ANNOTATE.out.vcf_tbi) + ch_reports = ch_reports.mix(SNPEFF_ANNOTATE.out.reports) ch_versions = ch_versions.mix(SNPEFF_ANNOTATE.out.versions.first()) } - if ('merge' in tools) { - vcf_ann_for_merge = snpeff_vcf_ann.map{ meta, vcf, tbi -> [meta, vcf, tbi] } - (merge_vcf_ann, merge_vep_report, merge_vep_version) = MERGE_ANNOTATE(vcf_ann_for_merge, vep_genome, vep_species, vep_cache_version, vep_cache) + if (tools.contains('merge')) { + vcf_ann_for_merge = SNPEFF_ANNOTATE.out.vcf_tbi.map{ meta, vcf, tbi -> [meta, vcf] } + MERGE_ANNOTATE(vcf_ann_for_merge, vep_genome, vep_species, vep_cache_version, vep_cache) + ch_vcf_ann = ch_vcf_ann.mix(MERGE_ANNOTATE.out.vcf_tbi) + ch_reports = ch_reports.mix(MERGE_ANNOTATE.out.reports) ch_versions = ch_versions.mix(MERGE_ANNOTATE.out.versions.first()) } - if ('vep' in tools) { - (vep_vcf_ann, vep_report, vep_version) = ENSEMBLVEP_ANNOTATE(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + if (tools.contains('vep')) { + ENSEMBLVEP_ANNOTATE(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + ch_vcf_ann = ch_vcf_ann.mix(ENSEMBLVEP_ANNOTATE.out.vcf_tbi) + ch_reports = ch_reports.mix(ENSEMBLVEP_ANNOTATE.out.reports) ch_versions = ch_versions.mix(ENSEMBLVEP_ANNOTATE.out.versions.first()) } - vcf_ann = snpeff_vcf_ann.mix(merge_vcf_ann, vep_vcf_ann) - reports = snpeff_report.mix(merge_vep_report, vep_report) - versions = ch_versions - emit: - reports - vcf_ann - versions + vcf_ann = ch_vcf_ann + reports = ch_reports + versions = ch_versions } diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index a4ab7c5d..b3e25c1a 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -89,8 +89,8 @@ workflow PREPARE_GENOME { // // Uncompress STAR index or generate from scratch if required // - ch_star_index = Channel.empty() - ch_fasta_for_index = Channel.empty() + + ch_star_index = Channel.empty() if ('star' in prepare_tool_indices) { if (params.star_index) { @@ -98,30 +98,17 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = Channel.fromPath(params.star_index) + ch_star_index = file(params.star_index) } - - // Get the version of the index and branch into valid or invalid - ch_star_index.branch { dir -> - valid_index: getIndexVersion(dir) == '2.7.4a' - return dir - invalid_index: getIndexVersion(dir) != '2.7.4a' - return ch_fasta - }.set{result} - - ch_star_index = result.valid_index - ch_fasta_for_index = result.invalid_index - } - else { - ch_fasta_for_index = ch_fasta } - ch_star_index_optional = STAR_GENOMEGENERATE (ch_fasta_for_index,ch_gtf).index - ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) - - ch_star_index = ch_star_index.mix(ch_star_index_optional) + if((!ch_star_index) || getIndexVersion(ch_star_index) != '2.7.4a'){ + ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + } } + emit: fasta = ch_fasta // path: genome.fasta fai = ch_fasta_fai // path: genome.fasta.fai diff --git a/subworkflows/local/splitncigar.nf b/subworkflows/local/splitncigar.nf index 483e18b1..db2dd73a 100644 --- a/subworkflows/local/splitncigar.nf +++ b/subworkflows/local/splitncigar.nf @@ -41,16 +41,14 @@ workflow SPLITNCIGAR { }.groupTuple().set{bam_splitncigar_interval} SAMTOOLS_MERGE(bam_splitncigar_interval, fasta) - bam_splitncigar_merged = SAMTOOLS_MERGE.out.bam + splitncigar_bam = SAMTOOLS_MERGE.out.bam ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) - SAMTOOLS_INDEX(bam_splitncigar_merged) - bam_splitncigar_merged_index = SAMTOOLS_INDEX.out.bai + SAMTOOLS_INDEX(splitncigar_bam) + splitncigar_bam_bai = splitncigar_bam.join(SAMTOOLS_INDEX.out.bai) ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) emit: - - bam = bam_splitncigar_merged - bai = bam_splitncigar_merged_index - versions = ch_versions + bam_bai = splitncigar_bam_bai + versions = ch_versions } diff --git a/subworkflows/nf-core/ensemblvep_annotate.nf b/subworkflows/nf-core/ensemblvep_annotate.nf index 97aa3baf..4be2cbb5 100644 --- a/subworkflows/nf-core/ensemblvep_annotate.nf +++ b/subworkflows/nf-core/ensemblvep_annotate.nf @@ -35,7 +35,7 @@ workflow ENSEMBLVEP_ANNOTATE { ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) emit: - vcf = TABIX_BGZIPTABIX.out.tbi // channel: [ val(meta), vcf, tbi ] - vep_report = ENSEMBLVEP.out.report // path: *.html - versions = ch_versions // channel: [versions.yml] + vcf_tbi = TABIX_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf, tbi ] + reports = ENSEMBLVEP.out.report // path: *.html + versions = ch_versions // channel: [versions.yml] } diff --git a/subworkflows/nf-core/snpeff_annotate.nf b/subworkflows/nf-core/snpeff_annotate.nf index 238eb20b..e9183e47 100644 --- a/subworkflows/nf-core/snpeff_annotate.nf +++ b/subworkflows/nf-core/snpeff_annotate.nf @@ -32,7 +32,7 @@ workflow SNPEFF_ANNOTATE { ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) emit: - vcf = TABIX_BGZIPTABIX.out.tbi // channel: [ val(meta), vcf, tbi ] - snpeff_report = SNPEFF.out.report // path: *.html - versions = ch_versions // channel: [versions.yml] + vcf_tbi = TABIX_BGZIPTABIX.out.gz_tbi // channel: [ val(meta), vcf, tbi ] + reports = SNPEFF.out.report // path: *.html + versions = ch_versions // channel: [versions.yml] } diff --git a/tests/csv/samplesheet_test.csv b/tests/csv/samplesheet_test.csv index 7131bd5d..81e928c9 100644 --- a/tests/csv/samplesheet_test.csv +++ b/tests/csv/samplesheet_test.csv @@ -1,2 +1,3 @@ sample,fastq_1,fastq_2,strandedness GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +TEST123,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse diff --git a/tests/test_default.yml b/tests/test_default.yml index 3a0c6847..36cb35fa 100644 --- a/tests/test_default.yml +++ b/tests/test_default.yml @@ -9,5 +9,5 @@ - path: results/preprocessing/GM12878/GM12878.recal.bam.bai - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz - - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz.tbi + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz.tbi diff --git a/tests/test_defaultmultiplesamples.yml b/tests/test_defaultmultiplesamples.yml new file mode 100644 index 00000000..b3a52842 --- /dev/null +++ b/tests/test_defaultmultiplesamples.yml @@ -0,0 +1,19 @@ +- name: Run default pipeline on all samples + command: nextflow run main.nf -profile test,docker + tags: + - defaultmultiplesamples + - preprocessing + files: + - path: results/reports/multiqc_report.html + - path: results/preprocessing/GM12878/GM12878.recal.bam + - path: results/preprocessing/GM12878/GM12878.recal.bam.bai + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi + - path: results/variant_calling/GM12878/GM12878.filtered.vcf.gz + - path: results/variant_calling/GM12878/GM12878.filtered.vcf.gz.tbi + - path: results/preprocessing/TEST123/TEST123.recal.bam + - path: results/preprocessing/TEST123/TEST123.recal.bam.bai + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.tbi + - path: results/variant_calling/TEST123/TEST123.filtered.vcf.gz + - path: results/variant_calling/TEST123/TEST123.filtered.vcf.gz.tbi diff --git a/tests/test_removeduplicates.yml b/tests/test_removeduplicates.yml index 08619c83..8889ad6a 100644 --- a/tests/test_removeduplicates.yml +++ b/tests/test_removeduplicates.yml @@ -9,5 +9,5 @@ - path: results/preprocessing/GM12878/GM12878.recal.bam.bai - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz - - path: results/variant_filtering/GM12878/GM12878.filtered.vcf.gz.tbi + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz.tbi diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 0844a3f7..298dddae 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -119,7 +119,6 @@ def seq_center = params.seq_center ? params.seq_center : [] //if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) // Initialize varaint annotation associated channels -def tools = params.annotate_tools ? params.annotate_tools.split(',').collect{it.trim().toLowerCase().replaceAll('-', '').replaceAll('_', '')} : [] def snpeff_db = params.snpeff_db ?: Channel.empty() def vep_cache_version = params.vep_cache_version ?: Channel.empty() def vep_genome = params.vep_genome ?: Channel.empty() @@ -164,7 +163,6 @@ def multiqc_report = [] workflow RNAVAR { ch_versions = Channel.empty() - ch_fastq = Channel.empty() // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -209,18 +207,25 @@ workflow RNAVAR { // // MODULE: Run FastQC // + FASTQC ( ch_cat_fastq ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + // // PREPARE THE INTERVAL LIST FROM GTF FILE + // + ch_interval_list = Channel.empty() GATK4_BEDTOINTERVALLIST(ch_genome_bed, PREPARE_GENOME.out.dict) ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions.first().ifEmpty(null)) + // // MODULE: IntervalListTools from GATK4 + // + ch_interval_list_split = Channel.empty() if (!params.skip_intervallisttools) { GATK4_INTERVALLISTTOOLS(ch_interval_list) @@ -231,6 +236,7 @@ workflow RNAVAR { // // SUBWORKFLOW: Alignment with STAR // + ch_genome_bam = Channel.empty() ch_genome_bam_index = Channel.empty() ch_samtools_stats = Channel.empty() @@ -271,21 +277,23 @@ workflow RNAVAR { // Subworkflow - SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). - bam_splitncigar = Channel.empty() + splitncigar_bam_bai = Channel.empty() SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) - bam_splitncigar = SPLITNCIGAR.out.bam.join(SPLITNCIGAR.out.bai, by: [0]) - ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) + splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai + ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() - //ch_interval_list_baserecalibrator = ch_interval_list.map{ meta, bed -> [bed] }.collect() - bam_splitncigar_interval = Channel.empty() - bam_splitncigar_interval = bam_splitncigar.join(ch_interval_list) + + ch_interval_list_recalib = ch_interval_list.map{ meta, bed -> [bed] }.flatten() + splitncigar_bam_bai.combine(ch_interval_list_recalib) + .map{ meta, bam, bai, interval -> [ meta, bam, bai, interval] + }.set{splitncigar_bam_bai_interval} GATK4_BASERECALIBRATOR( - bam_splitncigar_interval, + splitncigar_bam_bai_interval, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, @@ -296,16 +304,19 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) // MODULE: ApplyBaseRecalibrator from GATK4 - bam_applybqsr = bam_splitncigar.join(ch_bqsr_table, by: [0]) + bam_applybqsr = splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) bam_recalibrated = Channel.empty() bam_recalibrated_qc = Channel.empty() - //ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.collect() - bam_applybqsr.join(ch_interval_list) + + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.flatten() + bam_applybqsr.combine(ch_interval_list_applybqsr) + .map{ meta, bam, bai, table, interval -> [ meta, bam, bai, table, interval] + }.set{applybqsr_bam_bai_interval} RECALIBRATE( ('bamqc' in params.skip_qc), ('samtools' in params.skip_qc), - bam_applybqsr, + applybqsr_bam_bai_interval, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.fasta @@ -356,9 +367,10 @@ workflow RNAVAR { GATK4_INDEXFEATUREFILE( haplotypecaller_vcf ) + haplotypecaller_vcf = haplotypecaller_vcf //.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) haplotypecaller_vcf_tbi = haplotypecaller_vcf.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) - ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) - final_vcf_tbi = haplotypecaller_vcf_tbi + ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) + final_vcf = haplotypecaller_vcf // MODULE: VariantFiltration from GATK4 if (!params.skip_variantfiltration) { @@ -370,15 +382,15 @@ workflow RNAVAR { PREPARE_GENOME.out.dict ) - filtered_vcf_tbi = GATK4_VARIANTFILTRATION.out.vcf.join(GATK4_VARIANTFILTRATION.out.tbi, by: [0]) - final_vcf_tbi = filtered_vcf_tbi - ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) + filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf //.join(GATK4_VARIANTFILTRATION.out.tbi, by: [0]) + final_vcf = filtered_vcf + ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) } - if(!params.skip_variantannotation && ('merge' in tools || 'snpeff' in tools || 'vep' in tools)) { + if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('snpeff') || params.annotate_tools.contains('vep'))) { ANNOTATE( - final_vcf_tbi, - tools, + final_vcf, + params.annotate_tools, snpeff_db, snpeff_cache, vep_genome, From bff2abc449eb608b4f1a78824f595d2b698fb99a Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 14 Jan 2022 07:08:55 +0100 Subject: [PATCH 104/235] Add: multiplesamples CI test; code cleanup --- .github/workflows/ci.yml | 1 - tests/test_default.yml | 6 ++ tests/test_defaultmultiplesamples.yml | 19 ---- workflows/rnavar.nf | 127 ++++++++------------------ 4 files changed, 43 insertions(+), 110 deletions(-) delete mode 100644 tests/test_defaultmultiplesamples.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 44e10c1b..63d19123 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,6 @@ jobs: NXF_EDGE: '1' test: - 'default' - - 'defaultmultiplesamples' - 'annotation' - 'removeduplicates' steps: diff --git a/tests/test_default.yml b/tests/test_default.yml index 36cb35fa..79264af6 100644 --- a/tests/test_default.yml +++ b/tests/test_default.yml @@ -11,3 +11,9 @@ - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz.tbi + - path: results/preprocessing/TEST123/TEST123.recal.bam + - path: results/preprocessing/TEST123/TEST123.recal.bam.bai + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.tbi + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.filtered.vcf.gz + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.filtered.vcf.gz.tbi diff --git a/tests/test_defaultmultiplesamples.yml b/tests/test_defaultmultiplesamples.yml deleted file mode 100644 index b3a52842..00000000 --- a/tests/test_defaultmultiplesamples.yml +++ /dev/null @@ -1,19 +0,0 @@ -- name: Run default pipeline on all samples - command: nextflow run main.nf -profile test,docker - tags: - - defaultmultiplesamples - - preprocessing - files: - - path: results/reports/multiqc_report.html - - path: results/preprocessing/GM12878/GM12878.recal.bam - - path: results/preprocessing/GM12878/GM12878.recal.bam.bai - - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - - path: results/variant_calling/GM12878/GM12878.filtered.vcf.gz - - path: results/variant_calling/GM12878/GM12878.filtered.vcf.gz.tbi - - path: results/preprocessing/TEST123/TEST123.recal.bam - - path: results/preprocessing/TEST123/TEST123.recal.bam.bai - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.tbi - - path: results/variant_calling/TEST123/TEST123.filtered.vcf.gz - - path: results/variant_calling/TEST123/TEST123.filtered.vcf.gz.tbi diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 298dddae..89a5ee11 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -9,7 +9,6 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowRnavar.initialise(params, log) -// Check input path parameters to see if they exist // Check input path parameters to see if they exist def checkPathParamList = [ params.input, @@ -29,7 +28,6 @@ for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } - if(!params.star_index and !params.gtf){ exit 1, "GTF file is required to build a STAR reference index! Use option -gtf to provide a GTF file." } /* @@ -47,76 +45,50 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi ======================================================================================== */ -// Don't overwrite global params.modules, create a copy instead and use that within the main script. -// def modules = params.modules.clone() - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' //addParams( options: [:] ) +include { INPUT_CHECK } from '../subworkflows/local/input_check' // Validate the input samplesheet.csv and prepare input channels +include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' // Build the genome index and other reference files +include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' // Splits reads that contain Ns in their cigar string +include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' // Haplotyper caller that runs on split interval files +include { ANNOTATE } from '../subworkflows/local/annotate' // Annotate variants using snpEff or VEP or both /* ======================================================================================== - IMPORT NF-CORE MODULES/SUBWORKFLOWS + IMPORT NF-CORE MODULES ======================================================================================== */ -//def multiqc_options = modules['multiqc'] -//multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' //addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' //addParams( options: multiqc_options ) -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' //addParams( options: [publish_files : ['_versions.yml':'']] ) +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' +include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' /* ======================================================================================== - RUN MAIN WORKFLOW + IMPORT NF-CORE SUBWORKFLOWS ======================================================================================== */ -// Check alignment parameters -def prepareToolIndices = [] -prepareToolIndices = params.aligner - -//def publish_genome_options = params.save_reference ? [publish_dir: 'genome'] : [publish_files: false] -//def publish_index_options = params.save_reference ? [publish_dir: 'genome/index'] : [publish_files: false] -//def untar_options = [publish_files: false] -//def samtools_sort_genome_options = modules['samtools_sort_genome'] - -//if (!params.save_reference) modules['star_genomegenerate']['publish_files'] = false - -//modules['samtools_index_genome'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' - -//if (params.save_align_intermeds) { -// samtools_sort_genome_options.publish_files.put('bam','') -// samtools_sort_genome_options.publish_files.put('bai','') -// samtools_sort_genome_options.publish_files.put('csi','') -//} +include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' // Align reads to genome and sort and index the alignment file +include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' // Mark duplicates in the BAM file +include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' // Estimate and correct systematic bias -//if (!params.save_merged_fastq) modules['cat_fastq']['publish_files'] = false - -// Additional paramaters to modules based on params -//modules['star_align'].args += params.save_unaligned ? Utils.joinModuleArgs(['--outReadsUnmapped Fastx']) : '' -//modules['star_align'].args += params.star_twopass ? Utils.joinModuleArgs(['--twopassMode Basic']) : '' -def seq_platform = params.seq_platform ? params.seq_platform : [] -def seq_center = params.seq_center ? params.seq_center : [] - -//if (params.save_align_intermeds) modules['star_align'].publish_files.put('bam','') -//if (params.save_unaligned) modules['star_align'].publish_files.put('fastq.gz','unmapped') - -//modules['picard_markduplicates_samtools'].args += params.bam_csi_index ? Utils.joinModuleArgs(['-c']) : '' -//modules['picard_markduplicates'].args += params.remove_duplicates ? Utils.joinModuleArgs(['REMOVE_DUPLICATES=true']) : '' -//modules['gatk_intervallisttools'].args += Utils.joinModuleArgs(["--SCATTER_COUNT $params.scatter_count"]) - -//modules['gatk_haplotypecaller'].args += Utils.joinModuleArgs(["--standard-min-confidence-threshold-for-calling $params.stand_call_conf"]) +/* +======================================================================================== + VARIABLES +======================================================================================== +*/ -//if (params.window) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--window $params.window"]) -//if (params.cluster) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--cluster $params.cluster"]) -//if (params.fs_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"FS\" --filter \"FS > $params.fs_filter\" "]) -//if (params.qd_filter) modules['gatk_variantfilter'].args += Utils.joinModuleArgs(["--filter-name \"QD\" --filter \"QD < $params.qd_filter\" "]) +// Check STAR alignment parameters +def prepareToolIndices = params.aligner +def seq_platform = params.seq_platform ? params.seq_platform : [] +def seq_center = params.seq_center ? params.seq_center : [] // Initialize varaint annotation associated channels def snpeff_db = params.snpeff_db ?: Channel.empty() @@ -126,40 +98,15 @@ def vep_species = params.vep_species ?: Channel.empty() def snpeff_cache = params.snpeff_cache ? params.snpeff_cache : [] def vep_cache = params.vep_cache ? params.vep_cache : [] -// Include all the modules required for the pipeline -include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' //addParams(options: modules['cat_fastq']) -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' //addParams(options: modules['gatk_baserecalibrator']) -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' //addParams(options: modules['gatk_bedtointervallist']) -include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' //addParams(options: modules['gatk_haplotypecaller']) -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' //addParams(options: modules['gatk_intervallisttools']) -include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' //addParams(options: modules['gatk_mergevcfs']) -include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' //addParams(options: modules['gatk_indexfeaturefile']) -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' //addParams(options: modules['gatk_variantfilter']) -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' //addParams(options: modules['samtools_index_genome']) - -// Include all the subworkflows required for the pipeline - -// Build the genome index and other reference files -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' - -// Align reads to genome and sort and index the alignment file -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' - -// Mark duplicates in the BAM file -include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' - -// Subworkflow - splits reads that contain Ns in their cigar string -include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' - -// Estimate and correct systematic bias -include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' - -// Annotate variants using snpEff or VEP or both -include { ANNOTATE } from '../subworkflows/local/annotate' - -// Info required for completion email and summary +// MultiQC reporting def multiqc_report = [] +/* +======================================================================================== + RUN MAIN WORKFLOW RNAVAR +======================================================================================== +*/ + workflow RNAVAR { ch_versions = Channel.empty() From 98896efd682ce6b7352f00e21e8596fbe0f593c2 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 21 Jan 2022 12:15:36 +0100 Subject: [PATCH 105/235] Update docs --- conf/modules.config | 16 +-- conf/test.config | 7 +- docs/output.md | 91 ++++++++++++---- docs/usage.md | 149 +++++++++++++++++++++------ subworkflows/local/prepare_genome.nf | 15 ++- 5 files changed, 206 insertions(+), 72 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 3a8a38a5..c862beeb 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -86,15 +86,6 @@ process { ] } - withName: GET_CHROM_SIZES { - publishDir = [ - path: { "${params.outdir}/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - enabled: params.save_reference - ] - } - withName: GATK4_CREATESEQUENCEDICTIONARY { publishDir = [ path: { "${params.outdir}/genome" }, @@ -150,7 +141,7 @@ process { path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, pattern: '*.bam', - enabled: params.save_align_intermeds + enabled: false ], [ path: { "${params.outdir}/preprocessing/${meta.id}/unmapped" }, @@ -167,7 +158,7 @@ process { process { withName: '.*:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { - ext.suffix = '.sorted' + ext.prefix = {"${meta.id}.aligned"} publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, @@ -178,7 +169,7 @@ process { withName: '.*:ALIGN_STAR:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' - ext.suffix = '.sorted' + ext.prefix = {"${meta.id}.aligned"} publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, @@ -337,6 +328,7 @@ process { publishDir = [ path: { "${params.outdir}/variant_annotation/${meta.id}" }, enabled: true, + mode: params.publish_dir_mode, pattern: "*.{gz,gz.tbi}" ] } diff --git a/conf/test.config b/conf/test.config index 15dcf743..2253775f 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,10 +23,8 @@ params { input = "${baseDir}/tests/csv/samplesheet_test.csv" // Genome references - genome = 'WBcel235' + //genome = 'WBcel235' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' - fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' - dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' gene_bed = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.bed' @@ -37,7 +35,8 @@ params { known_indels_tbi = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' // STAR index (optional) - star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' + //star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' + star_index = '/home/praveen/star_index_ver_test/star_invalid/' // Annotation snpeff_db = 'WBcel235.99' diff --git a/docs/output.md b/docs/output.md index 376cfc01..5d5e37f8 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,9 +2,32 @@ ## Introduction -This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline based on public data. Most of the plots are taken from the MultiQC report, which summarizes results at the end of the pipeline. -The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. +### Dataset: + +RNAseq data has been taken from GM12878 GIAB sample SRA Accession [SRR5665260](https://www.ncbi.nlm.nih.gov/sra/?term=SRR5665260). The dataset has 38.6 million PE reads of read length 2x151 bp from NextSeq 500 sequencing platform. + +### Samplesheet: + +A sample sheet has been prepared in the following way to set the FASTQ files to run the analysis. + +```console +sample,fastq_1,fastq_2,strandedness +GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz,reverse +``` + +### Execution: + +The pipeline has been executed with the following command. + +```console +nextflow run nf-core/rnavar -profile munin,docker --input samplesheet.csv --genome GRCh38 --read_length 151 --annotate_tools merge +``` + +It used an institutional profile `munin` from [nf-core/configs](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config) + +The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. ## Pipeline overview @@ -35,35 +58,27 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Reference genome files](#reference-genome-files) - Saving reference genome indices/files * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC +## Preprocessing +### cat

Output files -* `fastqc/` - * `*_fastqc.html`: FastQC report containing quality metrics. - * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +* `fastq/` + * `*.merged.fastq.gz`: If `--save_merged_fastq` is specified, concatenated FastQ files will be placed in this directory.
-[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) - -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) - -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) +If multiple libraries/runs have been provided for the same sample in the input samplesheet (e.g. to increase sequencing depth) then these will be merged at the very beginning of the pipeline in order to have consistent sample naming throughout the pipeline. Please refer to the [usage documentation](https://nf-co.re/rnavar/usage#samplesheet-input) to see how to specify these samples in the input samplesheet. -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. - -### MultiQC +### MultiQC report with FASTQC read summary
Output files -* `multiqc/` +* `reports/` * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. * `multiqc_plots/`: directory containing static images from the report in various formats.
@@ -72,6 +87,46 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +## Alignment + +### STAR + +
+Output files + +* `preprocessing/[SAMPLE]/` + * `*.aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. + * `*.aligned.bam.bai`: This is the index of the above *.aligned.bam +* `preprocessing/[SAMPLE]/log` + * `*.Log.final.out`: STAR alignment report containing the mapping results summary. + * `*.Log.out` and `*.Log.progress.out`: STAR log files containing detailed information about the run. Typically only useful for debugging purposes. + * `*.SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. +* `preprocessing/[SAMPLE]/unmapped` + * `*.unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. +* `reports/samtools_stats/[SAMPLE]/` + * `*.aligned.bam.flagstat`: Samtools flagstat summary of the alignment + * `*.aligned.bam.stats`: Samtools stat output + +
+ +[STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. + +### MarkDuplicates + +
+Output files + +* `preprocessing/[SAMPLE]/` + * `*.markdup.sorted.bam`: Picard Markduplicate bam file. + * `*.markdup.sorted.bam.bai`: This is the index of the above *.aligned.bam +* `reports/samtools_stats/[SAMPLE]/` + * `*.markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment + * `*.markdup.sorted.bam.stats`: Samtools stat output + +
+ +[STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. + ### Pipeline information
diff --git a/docs/usage.md b/docs/usage.md index e6b2029c..bf994ca3 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -4,6 +4,40 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ +## RUNNING THE PIPELINE + +The typical command for running the pipeline is as follows: + +```console +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker +``` + +This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. + +Note that the pipeline will create the following files in your working directory: + +```console +work # Directory containing the nextflow working files +results # Finished results (configurable, see below) +.nextflow_log # Log file from Nextflow +# Other nextflow hidden files, eg. history of pipeline runs and old logs. +``` + +### Updating the pipeline + +When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: + +```console +nextflow pull nf-core/rnavar +``` + +### Reproducibility + +It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. + +First, go to the [nf-core/rnavar releases page](https://github.com/nf-core/rnavar/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. + +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. ## Samplesheet input You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. @@ -12,7 +46,7 @@ You will need to create a samplesheet with information about the samples you wou --input '[path to samplesheet file]' ``` -### Multiple runs of the same sample +#### Multiple runs of the same sample The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: @@ -23,7 +57,7 @@ CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz,u CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz,unstranded ``` -### Full samplesheet +#### Full samplesheet The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. @@ -51,56 +85,113 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p > **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. -## Alignment options +## PIPELINE PARAMETERS AND DESCRIPTION +## Reference genome files -The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome. STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. +The minimum reference genome requirements are a FASTA and GTF file, all other files required to run the pipeline can be generated from these files. However, it is more storage and compute friendly if you are able to re-use reference genome files as efficiently as possible. It is recommended to use the `--save_reference` parameter if you are using the pipeline to build new indices (e.g. those unavailable on [AWS iGenomes](https://nf-co.re/usage/reference_genomes)) so that you can save them somewhere locally. -## Reference genome files +> **NB:** Compressed reference files are also supported by the pipeline i.e. standard files with the `.gz` extension and indices folders with the `tar.gz` extension. + +The index building step can be quite a time-consuming process and it permits their reuse for future runs of the pipeline to save disk space. You can then either provide the appropriate reference genome files on the command-line via the appropriate parameters (e.g. `--star_index '/path/to/STAR/index/'`) or via a custom config file. -The minimum reference genome requirements are a FASTA and GTF file, all other files required to run the pipeline can be generated from these files. However, it is more storage and compute friendly if you are able to re-use reference genome files as efficiently as possible. It is recommended to use the `--save_reference` parameter if you are using the pipeline to build new indices (e.g. those unavailable on [AWS iGenomes](https://nf-co.re/usage/reference_genomes)) so that you can save them somewhere locally. The index building step can be quite a time-consuming process and it permits their reuse for future runs of the pipeline to save disk space. You can then either provide the appropriate reference genome files on the command-line via the appropriate parameters (e.g. `--star_index '/path/to/STAR/index/'`) or via a custom config file. +> **NB:** If you are supplying a pre-built genome index file via `--star_index`, please ensure that the index has been generated with the latest STAR version i.e. v2.7.9a or above. In case if the pipeline found an incompatible index, it will generate a new one using the reference genome which will consume time and memory unnecessarily. * If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. * If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. * If `--gene_bed` is not provided then it will be generated from the GTF file. +* If `--star_index` is not provided then it will be generated from the reference genome FASTA file using `STAR --runmode genomeGenerate` command. -> **NB:** Compressed reference files are also supported by the pipeline i.e. standard files with the `.gz` extension and indices folders with the `tar.gz` extension. +> **NB:** In case if you are providing a GTF and/or a BED file, please ensure that the chromosomes and contigs in the files are also present in the genome FASTA (and in the .dict) file. Otherwise `GATK BedToIntervalList` module is likely to fail if the chromosomes/contigs do not match with the reference genome data. -## Running the pipeline +## Alignment options -The typical command for running the pipeline is as follows: +The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome. STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. -```console -nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker -``` +By default, STAR runs in `2-pass` mode. For the most sensitive novel junction discovery, it is recommend running STAR in the 2-pass +mode. It does not increase the number of detected novel junctions, but allows to detect more splices reads mapping to novel junctions. The basic idea is to run 1st pass of STAR mapping with the usual parameters, then collect the junctions detected in the first pass, and use them as ”annotated” junctions for the 2nd pass mapping. You can turn off this feature by setting `--star_twopass false` in command line. -This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. +Read length is an important parameter therefore it has to be used carefully. The default is set to 150, but it has to be changed according to the input reads. For example, if the input read length is 2x151bp, then you use `--read_length 151`. The `--read_length` parameter is used while generating an index as well as in the alignment process. In both processes, the pipeline use (read_length - 1) to the STAR parameter `--sjdbOverhang` as recommended in STAR documentation. -Note that the pipeline will create the following files in your working directory: +> **NB:** Read length `--read_length` is an important parameter, therefore it has to set according to the input read length. If you are supplying a pre-built genome index, please make sure that you have used the same (read_length -1) during the genomeGenerate step. -```console -work # Directory containing the nextflow working files -results # Finished results (configurable, see below) -.nextflow_log # Log file from Nextflow -# Other nextflow hidden files, eg. history of pipeline runs and old logs. +## Preprocessing options + +Marking duplicate reads is performed using `Picard MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. + +GATK best practices has been followed in this pipeline for RNA analysis, hence it uses GATK modules such as `SplitNCigarReads`, `BaseRecalibrator`, `ApplyBQSR`. The `BaseRecalibrator` process requires known variants sites VCF. ExAc, gnomAD, or dbSNP resources can be used as known sites of variation.You can supply the VCF and index files using parameters such as `--dbsnp`, `--dbsnp_tbi`, `--known_indels`, `--known_indels_tbi`. + +`GATK SplitNCigarReads` is very time consuming step, therefore we made an attempt to break the GTF file into multiple chunks (scatters) using `GATK IntervalListTools` to run the process independently on each chunk in a parallel way to speed up the analysis. The default number of splits is set to 25, that means the GTF file is split into 25 smaller files and run `GATK SplitNCigarReads` on each of them in parallel. You can modify the number of splits using parameter `--scatter_count`. + +## Variant calling and filtering + +`GATK HaplotypeCaller` is used for variant calling with default minimum phred-scaled confidence threshold as 20. This value can be changed using paramerter `--stand_call_conf`. + +The pipeline runs a hard-filtering step on the variants by default. It does not filter out any variants, rather it flags i.e. PASS or other flags such as FS, QD, SnpCluster, etc. in FILTER column of the VCF. The following are the default filter criteria, however it can be changed using the respective parameters. + +* `--cluster` is set to 3. It is the number of SNPs which make up a cluster. +* `--window` is set to 35. The window size (in bases) in which to evaluate clustered SNPs. +* `--fs_filter` is set to 30.0. Filter based on FisherStrand > 30.0. It is the Phred-scaled probability that there is strand bias at the site. +* `--qd_filter` is set to 2.0 meaning filter variants if Quality By Depth filter is < 2.0. + +Variant filtering is an optional step. You can skip it using `--skip_variantfiltration` parameter. + +## Variant annotation + +The annotation of variants is performed using snpEff and VEP. The parameter to use is `--annotate_tools snpeff` or `--annotate_tools vep`. You can even run both snpEff and VEP using `--annotate_tools merge`, in this case the output VCF file will have both snpEff and VEP annotations combined. + +You can skip the variant annotation step using `--skip_variantannotation` parameter or without passing `--annotate_tools` options. +### Annotation cache + +Both `snpEff` and `VEP` enable usage of cache. +If cache is available on the machine where `rnavar` is run, it is possible to run annotation using cache. +You need to specify the cache directory using `--snpeff_cache` and `--vep_cache` in the command lines or within configuration files. +The cache will only be used when `--annotation_cache` and cache directories are specified (either in command lines or in a configuration file). + +Example: + +```bash +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker --annoate_tools snpEff --snpeff_cache --annotation_cache +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker --annotate_tools VEP --vep_cache --annotation_cache ``` -### Updating the pipeline +### Download annotation cache -When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: +A `Nextflow` helper script has been designed to help downloading `snpEff` and `VEP` caches. +Such files are meant to be shared between multiple users, so this script is mainly meant for people administrating servers, clusters and advanced users. -```console -nextflow pull nf-core/rnavar +```bash +nextflow run download_cache.nf --snpeff_cache --snpeff_db --genome +nextflow run download_cache.nf --vep_cache --species --vep_cache_version --genome ``` -### Reproducibility +### Using VEP CADD plugin -It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. +To enable the use of the `VEP` `CADD` plugin: -First, go to the [nf-core/rnavar releases page](https://github.com/nf-core/rnavar/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. +* Download the `CADD` files +* Specify them (either on the command line, like in the example or in a configuration file) +* use the `--cadd_cache` flag -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. +Example: + +```bash +nextflow run nf-core/sarek --input samplesheet.csv --genome GRCh38 -profile docker --annotate_tools VEP VEP --cadd_cache \ + --cadd_indels \ + --cadd_indels_tbi \ + --cadd_wg_snvs \ + --cadd_wg_snvs_tbi +``` + +### Downloading CADD files + +An helper script has been designed to help downloading `CADD` files. +Such files are meant to be share between multiple users, so this script is mainly meant for people administrating servers, clusters and advanced users. + +```bash +nextflow run download_cache.nf --cadd_cache --cadd_version --genome +``` -## Core Nextflow arguments +## GENERAL NEXTFLOW AGRUMENTS > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). @@ -145,7 +236,7 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. -## Custom configuration +## CUSTOME CONFIGURATIONS ### Resource requests diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index b3e25c1a..681797b9 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -3,7 +3,6 @@ // include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' //addParams(options: params.genome_options) -include { GET_CHROM_SIZES } from '../../modules/local/get_chrom_sizes' //addParams(options: params.genome_options) include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' //addParams(options: params.gffread_options) include { GTF2BED } from '../../modules/local/gtf2bed' //addParams(options: params.genome_options) include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) @@ -74,17 +73,16 @@ workflow PREPARE_GENOME { // Index the genome fasta ch_fasta_fai = Channel.empty() if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) - else ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai + if (!params.fasta_fai) { + SAMTOOLS_FAIDX(Channel.fromPath(ch_fasta).map{ it -> [[id:it[0].getName()], it]}) + ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] } + ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) + } // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() if (params.dict) ch_fasta_dict = file(params.dict) - else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict - - // - // Create chromosome sizes file - // - ch_chrom_sizes = GET_CHROM_SIZES ( ch_fasta ).sizes + else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict // // Uncompress STAR index or generate from scratch if required @@ -115,7 +113,6 @@ workflow PREPARE_GENOME { dict = ch_fasta_dict // path: genome.fasta.dict gtf = ch_gtf // path: genome.gtf gene_bed = ch_gene_bed // path: gene.bed - chrom_sizes = ch_chrom_sizes // path: genome.sizes star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } From a91d18853b7ec77b054d1ed468f860a8bc90c164 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 21 Jan 2022 12:24:18 +0100 Subject: [PATCH 106/235] Fix: samtools faidx issue with genome.fa --- conf/test.config | 2 +- subworkflows/local/prepare_genome.nf | 6 +++++- 2 files changed, 6 insertions(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index 15dcf743..a947f2a5 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,7 +25,7 @@ params { // Genome references genome = 'WBcel235' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' - fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' + //fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' gene_bed = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.bed' diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index b3e25c1a..e6b46953 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -74,7 +74,11 @@ workflow PREPARE_GENOME { // Index the genome fasta ch_fasta_fai = Channel.empty() if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) - else ch_fasta_fai = SAMTOOLS_FAIDX(ch_fasta).fai + if (!params.fasta_fai) { + SAMTOOLS_FAIDX(Channel.fromPath(ch_fasta).map{ it -> [[id:it[0].getName()], it]}) + ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] } + ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) + } // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() From 09b617f1a3fb8f56f7c2973a250a188c32e2736e Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 21 Jan 2022 14:22:09 +0100 Subject: [PATCH 107/235] pytest check --- conf/modules.config | 4 ++++ subworkflows/local/prepare_genome.nf | 4 ++-- 2 files changed, 6 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 3a8a38a5..defc2427 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -86,6 +86,10 @@ process { ] } + withName: SAMTOOLS_FAIDX { + publishDir = [ enabled: false ] + } + withName: GET_CHROM_SIZES { publishDir = [ path: { "${params.outdir}/genome" }, diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index e6b46953..2d06c326 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -76,8 +76,8 @@ workflow PREPARE_GENOME { if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) if (!params.fasta_fai) { SAMTOOLS_FAIDX(Channel.fromPath(ch_fasta).map{ it -> [[id:it[0].getName()], it]}) - ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] } - ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) + ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] }.collect() + ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) } // Create dictionary file for the genome fasta From 34c70dd6940fcb6e4a15705e02c41fe5da18c56b Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 21 Jan 2022 14:25:06 +0100 Subject: [PATCH 108/235] update: modules.config --- conf/modules.config | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index defc2427..852900e4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -87,7 +87,12 @@ process { } withName: SAMTOOLS_FAIDX { - publishDir = [ enabled: false ] + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference + ] } withName: GET_CHROM_SIZES { From df8897a890c3b856b2cd472a59787e9d522b7093 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 31 Jan 2022 16:26:54 +0100 Subject: [PATCH 109/235] ifix --- conf/test.config | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index 2253775f..513120ac 100644 --- a/conf/test.config +++ b/conf/test.config @@ -35,8 +35,7 @@ params { known_indels_tbi = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi' // STAR index (optional) - //star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' - star_index = '/home/praveen/star_index_ver_test/star_invalid/' + star_index = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/index/star/star.tar.gz' // Annotation snpeff_db = 'WBcel235.99' From e46cb94b8acc18e71b83106f8d339dc8ee71e706 Mon Sep 17 00:00:00 2001 From: m3hdad Date: Tue, 1 Feb 2022 15:56:52 +0100 Subject: [PATCH 110/235] snp/indel databases are required [issue 001] --- nextflow_schema.json | 8 +++++++- 1 file changed, 7 insertions(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 5ab8c5cc..1adceaf8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -161,7 +161,13 @@ "fa_icon": "fas fa-tag", "description": "VEP cache version" } - } + }, + "required": [ + "dbsnp", + "dbsnp_tbi", + "known_indels_tbi", + "known_indels" + ] }, "alignment_options": { "title": "Alignment options", From 6aff6d86ea628a03100a96e37e1c9e541e961ca2 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 15:26:20 +0100 Subject: [PATCH 111/235] update: usage and output docs --- conf/modules.config | 9 --- docs/output.md | 165 +++++++++++++++++++++++++++++++++++++------ nextflow_schema.json | 11 +-- 3 files changed, 144 insertions(+), 41 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a4399c35..7c2952ca 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -95,15 +95,6 @@ process { ] } - withName: GET_CHROM_SIZES { - publishDir = [ - path: { "${params.outdir}/genome" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - enabled: params.save_reference - ] - } - withName: GATK4_CREATESEQUENCEDICTIONARY { publishDir = [ path: { "${params.outdir}/genome" }, diff --git a/docs/output.md b/docs/output.md index 5d5e37f8..6ab12f98 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,8 +2,7 @@ ## Introduction -This document describes the output produced by the pipeline based on public data. Most of the plots are taken from the MultiQC report, which summarizes results at the end of the pipeline. - +This document describes the output produced by the pipeline based on public data. ### Dataset: RNAseq data has been taken from GM12878 GIAB sample SRA Accession [SRR5665260](https://www.ncbi.nlm.nih.gov/sra/?term=SRR5665260). The dataset has 38.6 million PE reads of read length 2x151 bp from NextSeq 500 sequencing platform. @@ -16,7 +15,6 @@ A sample sheet has been prepared in the following way to set the FASTQ files to sample,fastq_1,fastq_2,strandedness GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz,reverse ``` - ### Execution: The pipeline has been executed with the following command. @@ -73,6 +71,10 @@ If multiple libraries/runs have been provided for the same sample in the input s ### MultiQC report with FASTQC read summary +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +
Output files @@ -83,51 +85,168 @@ If multiple libraries/runs have been provided for the same sample in the input s
-[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. - -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . ## Alignment - ### STAR +[STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. +
Output files * `preprocessing/[SAMPLE]/` - * `*.aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. - * `*.aligned.bam.bai`: This is the index of the above *.aligned.bam + * `[SAMPLE].aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. + * `[SAMPLE].aligned.bam.bai`: This is the index of the above *.aligned.bam * `preprocessing/[SAMPLE]/log` - * `*.Log.final.out`: STAR alignment report containing the mapping results summary. - * `*.Log.out` and `*.Log.progress.out`: STAR log files containing detailed information about the run. Typically only useful for debugging purposes. - * `*.SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. + * `[SAMPLE].Log.final.out`: STAR alignment report containing the mapping results summary. + * `[SAMPLE].Log.out` and `[SAMPLE].Log.progress.out`: STAR log files containing detailed information about the run. Typically only useful for debugging purposes. + * `[SAMPLE].SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. * `preprocessing/[SAMPLE]/unmapped` - * `*.unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. + * `[SAMPLE].unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. * `reports/samtools_stats/[SAMPLE]/` - * `*.aligned.bam.flagstat`: Samtools flagstat summary of the alignment - * `*.aligned.bam.stats`: Samtools stat output + * `[SAMPLE].aligned.bam.flagstat`: Samtools flagstat summary of the alignment + * `[SAMPLE].aligned.bam.stats`: Samtools stat output
-[STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. +## Preprocessing +### GATK MarkDuplicates + +[GATK MarkDuplicates](https://gatk.broadinstitute.org/hc/en-us/articles/360042477492-MarkDuplicates-Picard) locates and tags duplicate reads in a `BAM` file. The tool's main output is a new BAM file, in which duplicates have been identified in the SAM flags field for each read. -### MarkDuplicates +If desired, duplicates can be removed using the `--remove_duplicates true` option.
Output files * `preprocessing/[SAMPLE]/` - * `*.markdup.sorted.bam`: Picard Markduplicate bam file. - * `*.markdup.sorted.bam.bai`: This is the index of the above *.aligned.bam + * `[SAMPLE].markdup.sorted.bam`: Picard Markduplicate bam file. + * `[SAMPLE].markdup.sorted.bam.bai`: This is the index of the above *.aligned.bam * `reports/samtools_stats/[SAMPLE]/` - * `*.markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment - * `*.markdup.sorted.bam.stats`: Samtools stat output + * `[SAMPLE].markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment + * `[SAMPLE].markdup.sorted.bam.stats`: Samtools stat output
-[STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. +### SplitNCigarReads + +[GATK SplitNCigarReads](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads) is executed for post-processing RNA reads aligned against the reference. The tool's main output is a new BAM file with reads split at N CIGAR elements and CIGAR strings updated. + +Currently, the pipeline does not produce the new BAM file in the output directory. +### Base (Quality Score) Recalibration +#### GATK BaseRecalibrator + +[GATK BaseRecalibrator](https://gatk.broadinstitute.org/hc/en-us/articles/360042477672-BaseRecalibrator) generates a recalibration table based on various co-variates. + +Currently, the pipeline does not produce the recalibration table file in the output directory. +#### GATK ApplyBQSR + +[GATK ApplyBQSR](https://gatk.broadinstitute.org/hc/en-us/articles/360042476852-ApplyBQSR) recalibrates the base qualities of the input reads based on the recalibration table produced by the [GATK BaseRecalibrator](#gatk-baserecalibrator) tool. + +
+Output files + +* `preprocessing/[SAMPLE]/` + * `[SAMPLE].recal.bam`: Recalibrated bam file. + * `[SAMPLE].recal.bam.bai`: This is the index of the above recalibrated bam. + +
+ +## Variant calling + +[GATK HaplotypeCaller](https://gatk.broadinstitute.org/hc/en-us/articles/4414586765723-HaplotypeCaller) is used to call SNVs and small indels in the sample. The `Recalibrated BAM` file is used as an input to this process and the output file is produced in VCF format. + +
+Output files + +* `results/variant_calling/[SAMPLE]/` + * `[SAMPLE].haplotypecaller.vcf.gz`: Variant calls in VCF format. + * `[SAMPLE].haplotypecaller.vcf.gz.tbi`: This is the index of the above VCF file. + +
+ +## Variant filtering + +[GATK VariantFiltration](https://gatk.broadinstitute.org/hc/en-us/articles/360037434691-VariantFiltration) is used for hard-filtering variant calls based on certain criteria. Records are hard-filtered by changing the value in the FILTER field to something other than PASS. Filtered records will be preserved in the output. + +
+Output files + +* `results/variant_calling/[SAMPLE]/` + * `[SAMPLE].haplotypecaller.filtered.vcf.gz`: Variant VCF with updated FILTER field. + * `[SAMPLE].haplotypecaller.filtered.vcf.gz.tbi`: This is the index of the above VCF file. + +
+ +## Variant annotation + +This directory contains results from the final annotation steps: two tools are used for annotation, [snpEff](http://snpeff.sourceforge.net/) and [VEP](https://www.ensembl.org/info/docs/tools/vep/index.html). + +### snpEff + +[snpeff](http://snpeff.sourceforge.net/) is a genetic variant annotation and effect prediction toolbox. +It annotates and predicts the effects of variants on genes (such as amino acid changes) using multiple databases for annotations. +The generated `VCF` header contains the software version and the used command line. + +To annotate variants using `snpeff`, you can use `--annotate_tools snpeff` or `--annotate_tools merge`. +The annotated variant files in VCF format can be found in `results/variant_annotation` folder. + +
+Output files + +* `results/variant_annotation/[SAMPLE]/` + * `[SAMPLE]_snpEff.ann.vcf.gz`: Annotated VCF from snpEff process. + * `[SAMPLE]_snpEff.ann.vcf.gz.tbi`: This is the index of the above VCF file. + +
+ +For further reading and documentation see the [snpEff manual](http://snpeff.sourceforge.net/SnpEff_manual.html#outputSummary) + +### VEP + +[VEP (Variant Effect Predictor)](https://www.ensembl.org/info/docs/tools/vep/index.html), based on `Ensembl`, is a tool to determine the effects of the variants. The generated `VCF` header contains the software version, also the version numbers for additional databases like `Clinvar` or `dbSNP` used in the `VEP` line. +The format of the [consequence annotations](https://www.ensembl.org/info/genome/variation/prediction/predicted_data.html) is also in the `VCF` header describing the `INFO` field. + +Currently, it contains: + +- *Consequence*: impact of the variation, if there is any +- *Codons*: the codon change, i.e. cGt/cAt +- *Amino_acids*: change in amino acids, i.e. R/H if there is any +- *Gene*: ENSEMBL gene name +- *SYMBOL*: gene symbol +- *Feature*: actual transcript name +- *EXON*: affected exon +- *PolyPhen*: prediction based on [PolyPhen](http://genetics.bwh.harvard.edu/pph2/) +- *SIFT*: prediction by [SIFT](http://sift.bii.a-star.edu.sg/) +- *Protein_position*: Relative position of amino acid in protein +- *BIOTYPE*: Biotype of transcript or regulatory feature + +To annotate variants using `vep`, you can use `--annotate_tools vep`. +The annotated variant files in VCF format can be found in `results/variant_annotation` folder. + +
+Output files + +* `results/variant_annotation/[SAMPLE]/` + * `[SAMPLE]_VEP.ann.vcf.gz`: Annotated VCF from VEP process. + * `[SAMPLE]_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file. + +
+ +When `--annotate_tools merge` option is used, the annotation from both `snpeff` and `vep` are combined into a single VCF file which can be found with the following naming convention. + +
+Output files + +* `results/variant_annotation/[SAMPLE]/` + * `[SAMPLE]_snpEff_VEP.ann.vcf.gz`: Combined annotation from both snpEff and VEP. + * `[SAMPLE]_snpEff_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file. + +
+ +For further reading and documentation see the [VEP manual](https://www.ensembl.org/info/docs/tools/vep/index.html) -### Pipeline information +## Pipeline information
Output files diff --git a/nextflow_schema.json b/nextflow_schema.json index 5ab8c5cc..26c96426 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -44,7 +44,6 @@ }, "save_merged_fastq": { "type": "boolean", - "default": false, "description": "Save FastQ files after merging re-sequenced libraries in the results directory." } } @@ -119,7 +118,6 @@ }, "save_reference": { "type": "boolean", - "default": false, "help_text": "If the STAR index is generated by the pipeline, then please use this parameter to save it to your results folder. These index can then be used for future pipeline runs, reducing processing times.", "description": "If generated by the pipeline, save the STAR index in the results directory." }, @@ -187,7 +185,6 @@ }, "star_ignore_sjdbgtf": { "type": "boolean", - "default": false, "description": "Do not use GTF file during STAR index buidling step", "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." }, @@ -204,13 +201,11 @@ }, "save_unaligned": { "type": "boolean", - "default": false, "description": "Where possible, save unaligned reads from aligner to the results directory.", "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, "save_align_intermeds": { "type": "boolean", - "default": false, "description": "Save the intermediate BAM files from the alignment step.", "help_text": "By default, intermediate BAM files will not be saved. The final BAM files created after the appropriate filtering step are always saved to limit storage usage. Set this parameter to also save other intermediate BAM files." }, @@ -350,7 +345,6 @@ }, "skip_qc": { "type": "boolean", - "default": false, "description": "Skip QC reports", "help_text": "This parameter disable all QC reports from recalibrated BAM file." } @@ -371,8 +365,7 @@ "no_intervals": { "type": "boolean", "description": "Do not use gene interval file during variant calling", - "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False", - "default": "False" + "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False" }, "window": { "type": "integer", @@ -622,4 +615,4 @@ "aligner", "seq_platform" ] -} +} \ No newline at end of file From 02dce5bd7c21ffaccbad4e6a2e3840deb04bb67d Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 15:31:44 +0100 Subject: [PATCH 112/235] update: test.config --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 16ba99e9..a947f2a5 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,7 +23,7 @@ params { input = "${baseDir}/tests/csv/samplesheet_test.csv" // Genome references - //genome = 'WBcel235' + genome = 'WBcel235' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' //fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' From 06d911d7f311e444f69d6ca279b8f9e0d7ad5a50 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 15:33:38 +0100 Subject: [PATCH 113/235] removed: get_chrom_sizes.nf file --- modules/local/get_chrom_sizes.nf | 41 -------------------------------- 1 file changed, 41 deletions(-) delete mode 100644 modules/local/get_chrom_sizes.nf diff --git a/modules/local/get_chrom_sizes.nf b/modules/local/get_chrom_sizes.nf deleted file mode 100644 index 1f582d8c..00000000 --- a/modules/local/get_chrom_sizes.nf +++ /dev/null @@ -1,41 +0,0 @@ -// Import generic module functions -include { saveFiles; getProcessName } from './functions' - -params.options = [:] - -process GET_CHROM_SIZES { - tag "$fasta" - label "process_medium" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0" - } else { - container "quay.io/biocontainers/samtools:1.14--hb421002_0" - } - - input: - path fasta - - output: - path "*.sizes" , emit: sizes - path "*.fai" , emit: fai - path "versions.yml", emit: versions - - script: - """ - samtools \\ - faidx \\ - $fasta - - cut -f 1,2 ${fasta}.fai > ${fasta}.sizes - - cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - END_VERSIONS - """ -} From cfbdcb84648d1fc488d6a37bb6cbc2f704e3c77e Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 15:53:01 +0100 Subject: [PATCH 114/235] fix: markdownlint errors --- docs/output.md | 46 ++++++++++++++++++++++++++-------------------- 1 file changed, 26 insertions(+), 20 deletions(-) diff --git a/docs/output.md b/docs/output.md index 6ab12f98..7c017bae 100644 --- a/docs/output.md +++ b/docs/output.md @@ -3,11 +3,12 @@ ## Introduction This document describes the output produced by the pipeline based on public data. -### Dataset: + +### Dataset RNAseq data has been taken from GM12878 GIAB sample SRA Accession [SRR5665260](https://www.ncbi.nlm.nih.gov/sra/?term=SRR5665260). The dataset has 38.6 million PE reads of read length 2x151 bp from NextSeq 500 sequencing platform. -### Samplesheet: +### Samplesheet A sample sheet has been prepared in the following way to set the FASTQ files to run the analysis. @@ -15,7 +16,8 @@ A sample sheet has been prepared in the following way to set the FASTQ files to sample,fastq_1,fastq_2,strandedness GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz,reverse ``` -### Execution: + +### Execution The pipeline has been executed with the following command. @@ -57,6 +59,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ## Preprocessing + ### cat
@@ -87,6 +90,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ ## Alignment + ### STAR [STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. @@ -109,8 +113,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
-## Preprocessing -### GATK MarkDuplicates +## MarkDuplicates [GATK MarkDuplicates](https://gatk.broadinstitute.org/hc/en-us/articles/360042477492-MarkDuplicates-Picard) locates and tags duplicate reads in a `BAM` file. The tool's main output is a new BAM file, in which duplicates have been identified in the SAM flags field for each read. @@ -128,18 +131,21 @@ If desired, duplicates can be removed using the `--remove_duplicates true` optio
-### SplitNCigarReads +## SplitNCigarReads [GATK SplitNCigarReads](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads) is executed for post-processing RNA reads aligned against the reference. The tool's main output is a new BAM file with reads split at N CIGAR elements and CIGAR strings updated. Currently, the pipeline does not produce the new BAM file in the output directory. -### Base (Quality Score) Recalibration -#### GATK BaseRecalibrator + +## Base (Quality Score) Recalibration + +### GATK BaseRecalibrator [GATK BaseRecalibrator](https://gatk.broadinstitute.org/hc/en-us/articles/360042477672-BaseRecalibrator) generates a recalibration table based on various co-variates. Currently, the pipeline does not produce the recalibration table file in the output directory. -#### GATK ApplyBQSR + +### GATK ApplyBQSR [GATK ApplyBQSR](https://gatk.broadinstitute.org/hc/en-us/articles/360042476852-ApplyBQSR) recalibrates the base qualities of the input reads based on the recalibration table produced by the [GATK BaseRecalibrator](#gatk-baserecalibrator) tool. @@ -209,17 +215,17 @@ The format of the [consequence annotations](https://www.ensembl.org/info/genome/ Currently, it contains: -- *Consequence*: impact of the variation, if there is any -- *Codons*: the codon change, i.e. cGt/cAt -- *Amino_acids*: change in amino acids, i.e. R/H if there is any -- *Gene*: ENSEMBL gene name -- *SYMBOL*: gene symbol -- *Feature*: actual transcript name -- *EXON*: affected exon -- *PolyPhen*: prediction based on [PolyPhen](http://genetics.bwh.harvard.edu/pph2/) -- *SIFT*: prediction by [SIFT](http://sift.bii.a-star.edu.sg/) -- *Protein_position*: Relative position of amino acid in protein -- *BIOTYPE*: Biotype of transcript or regulatory feature +* `Consequence`: impact of the variation, if there is any +* `Codons`: the codon change, i.e. cGt/cAt +* `Amino_acids`: change in amino acids, i.e. R/H if there is any +* `Gene`: ENSEMBL gene name +* `SYMBOL`: gene symbol +* `Feature`: actual transcript name +* `EXON`: affected exon +* `PolyPhen`: prediction based on [PolyPhen](http://genetics.bwh.harvard.edu/pph2/) +* `SIFT`: prediction by [SIFT](http://sift.bii.a-star.edu.sg/) +* `Protein_position`: Relative position of amino acid in protein +* `BIOTYPE`: Biotype of transcript or regulatory feature To annotate variants using `vep`, you can use `--annotate_tools vep`. The annotated variant files in VCF format can be found in `results/variant_annotation` folder. From 069521b5079a03fb39472797d41643cc7302884b Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 15:58:38 +0100 Subject: [PATCH 115/235] fix: markdownlint errors - attempt 2 --- docs/usage.md | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index bf994ca3..9d60ce80 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -38,6 +38,7 @@ It is a good idea to specify a pipeline version when running the pipeline on you First, go to the [nf-core/rnavar releases page](https://github.com/nf-core/rnavar/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. + ## Samplesheet input You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. @@ -46,7 +47,7 @@ You will need to create a samplesheet with information about the samples you wou --input '[path to samplesheet file]' ``` -#### Multiple runs of the same sample +### Multiple runs of the same sample The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: @@ -86,6 +87,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p > **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. ## PIPELINE PARAMETERS AND DESCRIPTION + ## Reference genome files The minimum reference genome requirements are a FASTA and GTF file, all other files required to run the pipeline can be generated from these files. However, it is more storage and compute friendly if you are able to re-use reference genome files as efficiently as possible. It is recommended to use the `--save_reference` parameter if you are using the pipeline to build new indices (e.g. those unavailable on [AWS iGenomes](https://nf-co.re/usage/reference_genomes)) so that you can save them somewhere locally. @@ -140,6 +142,7 @@ Variant filtering is an optional step. You can skip it using `--skip_variantfilt The annotation of variants is performed using snpEff and VEP. The parameter to use is `--annotate_tools snpeff` or `--annotate_tools vep`. You can even run both snpEff and VEP using `--annotate_tools merge`, in this case the output VCF file will have both snpEff and VEP annotations combined. You can skip the variant annotation step using `--skip_variantannotation` parameter or without passing `--annotate_tools` options. + ### Annotation cache Both `snpEff` and `VEP` enable usage of cache. From 98bf7ae0981134a1f6b91556f10671b6fa0a0389 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 16:00:21 +0100 Subject: [PATCH 116/235] fix: markdownlint errors - attempt 3 --- docs/output.md | 2 -- 1 file changed, 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index 7c017bae..5ecac6ca 100644 --- a/docs/output.md +++ b/docs/output.md @@ -87,8 +87,6 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ * `multiqc_plots/`: directory containing static images from the report in various formats.
- - ## Alignment ### STAR From 7b7be1f16f42cc16b30968f9259eadc66f118184 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 16:17:39 +0100 Subject: [PATCH 117/235] update: output.md --- docs/output.md | 23 +++++++++++------------ 1 file changed, 11 insertions(+), 12 deletions(-) diff --git a/docs/output.md b/docs/output.md index 5ecac6ca..9e7a9390 100644 --- a/docs/output.md +++ b/docs/output.md @@ -39,17 +39,11 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Alignment](#alignment) * [STAR](#star) - Fast spliced aware genome alignment * [Alignment post-processing](#alignment-post-processing) - * [SAMtools](#samtools) - Sort and index alignments * [Picard MarkDuplicates](#picard-markduplicates) - Duplicate read marking * [GATK4 SplitNCigarReads](#gatk4-splitncigarreads) - Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data) * [GATK4 Base Quality Score Recalibration (BQSR)](#gatk4-bqsr) - Estimate and correct systematic bias that affect the assignment of base quality scores by the sequencer -* [Other steps](#other-steps) - * [Picard BedToIntervalList](#picard-bedtointervallist) - Converts a BED file to a Picard Interval List - * [Picard IntervalListTools](#picard-intervallisttools) - Scatter one interval-list into many interval-files to run analysis in parallel * [Variant calling](#other-steps) * [GATK4 HaplotypeCaller](#gatk4-haplotypecaller) - Call SNPs and indels via local re-assembly of haplotypes - * [GATK4 MergeVCFs](#gatk4-mergevcf) - Merge multiple VCF files into one VCF - * [GATK4 IndexFeatureFile](#gatk4-indexfeaturefile) - Index the VCF * [Variant filtering](#other-steps) * [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria * [Quality control](#quality-control) @@ -76,7 +70,9 @@ If multiple libraries/runs have been provided for the same sample in the input s [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +It integrates [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) which gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see .
Output files @@ -87,6 +83,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ * `multiqc_plots/`: directory containing static images from the report in various formats.
+ ## Alignment ### STAR @@ -111,7 +108,9 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ -## MarkDuplicates +## Alignment post-processing + +### MarkDuplicates [GATK MarkDuplicates](https://gatk.broadinstitute.org/hc/en-us/articles/360042477492-MarkDuplicates-Picard) locates and tags duplicate reads in a `BAM` file. The tool's main output is a new BAM file, in which duplicates have been identified in the SAM flags field for each read. @@ -129,21 +128,21 @@ If desired, duplicates can be removed using the `--remove_duplicates true` optio -## SplitNCigarReads +### SplitNCigarReads [GATK SplitNCigarReads](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads) is executed for post-processing RNA reads aligned against the reference. The tool's main output is a new BAM file with reads split at N CIGAR elements and CIGAR strings updated. Currently, the pipeline does not produce the new BAM file in the output directory. -## Base (Quality Score) Recalibration +### Base (Quality Score) Recalibration -### GATK BaseRecalibrator +#### GATK BaseRecalibrator [GATK BaseRecalibrator](https://gatk.broadinstitute.org/hc/en-us/articles/360042477672-BaseRecalibrator) generates a recalibration table based on various co-variates. Currently, the pipeline does not produce the recalibration table file in the output directory. -### GATK ApplyBQSR +#### GATK ApplyBQSR [GATK ApplyBQSR](https://gatk.broadinstitute.org/hc/en-us/articles/360042476852-ApplyBQSR) recalibrates the base qualities of the input reads based on the recalibration table produced by the [GATK BaseRecalibrator](#gatk-baserecalibrator) tool. From 7923f321a342a728fa96d6d9672b29606652d566 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 22:04:04 +0100 Subject: [PATCH 118/235] corrections based on review --- docs/output.md | 40 +++++++++++++++++++--------------------- docs/usage.md | 4 ++-- 2 files changed, 21 insertions(+), 23 deletions(-) diff --git a/docs/output.md b/docs/output.md index 9e7a9390..6cc2c0b1 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,7 +2,7 @@ ## Introduction -This document describes the output produced by the pipeline based on public data. +This document describes the output produced by the pipeline based on public data. The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. ### Dataset @@ -27,8 +27,6 @@ nextflow run nf-core/rnavar -profile munin,docker --input samplesheet.csv --geno It used an institutional profile `munin` from [nf-core/configs](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config) -The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: @@ -66,24 +64,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d If multiple libraries/runs have been provided for the same sample in the input samplesheet (e.g. to increase sequencing depth) then these will be merged at the very beginning of the pipeline in order to have consistent sample naming throughout the pipeline. Please refer to the [usage documentation](https://nf-co.re/rnavar/usage#samplesheet-input) to see how to specify these samples in the input samplesheet. -### MultiQC report with FASTQC read summary - -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. - -It integrates [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) which gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . - -
-Output files - -* `reports/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. - -
- ## Alignment ### STAR @@ -249,6 +229,24 @@ When `--annotate_tools merge` option is used, the annotation from both `snpeff` For further reading and documentation see the [VEP manual](https://www.ensembl.org/info/docs/tools/vep/index.html) +### MultiQC report with FASTQC read summary + +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +It integrates [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) which gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +
+Output files + +* `reports/` + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats. + +
+ ## Pipeline information
diff --git a/docs/usage.md b/docs/usage.md index 9d60ce80..b17c7ce7 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -178,7 +178,7 @@ To enable the use of the `VEP` `CADD` plugin: Example: ```bash -nextflow run nf-core/sarek --input samplesheet.csv --genome GRCh38 -profile docker --annotate_tools VEP VEP --cadd_cache \ +nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker --annotate_tools VEP VEP --cadd_cache \ --cadd_indels \ --cadd_indels_tbi \ --cadd_wg_snvs \ @@ -194,7 +194,7 @@ Such files are meant to be share between multiple users, so this script is mainl nextflow run download_cache.nf --cadd_cache --cadd_version --genome ``` -## GENERAL NEXTFLOW AGRUMENTS +## GENERAL NEXTFLOW ARGUMENTS > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). From ec83b2ceb89f43a2f01a576125c1d5d55dd92363 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 2 Feb 2022 22:59:53 +0100 Subject: [PATCH 119/235] corrections based on review - 2 --- docs/output.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index 6cc2c0b1..c831811f 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,7 +2,9 @@ ## Introduction -This document describes the output produced by the pipeline based on public data. The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. +This document describes the output produced by the pipeline based on public data. + +The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. ### Dataset From 347021497ee209ade19f81973997fa67ee3c319b Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 03:43:14 +0100 Subject: [PATCH 120/235] added: skip_baserecalibration for non-model organisms --- .github/workflows/ci.yml | 1 + conf/modules.config | 73 ++++++++++++++------------- lib/WorkflowRnavar.groovy | 4 +- nextflow.config | 1 + nextflow_schema.json | 13 +++-- tests/test_skipbasecalib.yml | 11 +++++ workflows/rnavar.nf | 95 ++++++++++++++++++++---------------- 7 files changed, 113 insertions(+), 85 deletions(-) create mode 100644 tests/test_skipbasecalib.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 63d19123..5fc82bbe 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -30,6 +30,7 @@ jobs: - 'default' - 'annotation' - 'removeduplicates' + - 'skipbasecalib' steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/conf/modules.config b/conf/modules.config index 7c2952ca..03ad2989 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -231,43 +231,50 @@ process { publishDir = [ enabled: false ] } - withName: GATK4_BASERECALIBRATOR { - ext.args = '--use-original-qualities' - publishDir = [ enabled: false ] + withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { + publishDir = [ + path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, + mode: params.publish_dir_mode, + enabled: true, + pattern: "*.{stats,flagstat}" + ] } - withName: '.*:RECALIBRATE:APPLYBQSR' { - ext.args = [ - '--use-original-qualities', - '--add-output-sam-program-record' - ].join(' ').trim() - ext.prefix = {"${meta.id}.recal"} - publishDir = [ - path: { "${params.outdir}/preprocessing/${meta.id}" }, - mode: params.publish_dir_mode, - enabled: true, - pattern: "*.{bam}" - ] - } +} - withName: '.*:RECALIBRATE:SAMTOOLS_INDEX' { - ext.args = params.bam_csi_index ? '-c' : '' - ext.prefix = {"${meta.id}.recal"} - publishDir = [ - path: { "${params.outdir}/preprocessing/${meta.id}" }, - mode: params.publish_dir_mode, - enabled: true, - pattern: "*.{bai,csi}" - ] - } +// BASE RECALIBRATION PROCESS +if(!params.skip_baserecalibration) { + process { - withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { - publishDir = [ - path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, - mode: params.publish_dir_mode, - enabled: true, - pattern: "*.{stats,flagstat}" - ] + withName: GATK4_BASERECALIBRATOR { + ext.args = '--use-original-qualities' + publishDir = [ enabled: false ] + } + + withName: '.*:RECALIBRATE:APPLYBQSR' { + ext.args = [ + '--use-original-qualities', + '--add-output-sam-program-record' + ].join(' ').trim() + ext.prefix = {"${meta.id}.recal"} + publishDir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + enabled: true, + pattern: "*.{bam}" + ] + } + + withName: '.*:RECALIBRATE:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + ext.prefix = {"${meta.id}.recal"} + publishDir = [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + enabled: true, + pattern: "*.{bai,csi}" + ] + } } } diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index ab5cce51..b12a0ca2 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -20,8 +20,8 @@ class WorkflowRnavar { System.exit(1) } - if (!params.dbsnp || !params.dbsnp_tbi) { - log.error "dbSNP VCF file or its index is missing!. Use --dbsnp and --dbsnp_tbi to supply the files." + if ((!params.skip_baserecalibration) && (!params.known_indels || !params.known_indels_tbi)) { + log.error "Known variants VCF file or its index is missing!. Use --known_indels and --known_indels_tbi to supply the files." System.exit(1) } diff --git a/nextflow.config b/nextflow.config index e1a0762a..4a781da4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -53,6 +53,7 @@ params { vep_cache = null // No directory for VEP cache // Skip steps + skip_baserecalibration = false skip_intervallisttools = false skip_variantfiltration = false skip_variantannotation = false diff --git a/nextflow_schema.json b/nextflow_schema.json index dc18cb64..dc1392f1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -159,13 +159,7 @@ "fa_icon": "fas fa-tag", "description": "VEP cache version" } - }, - "required": [ - "dbsnp", - "dbsnp_tbi", - "known_indels_tbi", - "known_indels" - ] + } }, "alignment_options": { "title": "Alignment options", @@ -334,6 +328,11 @@ "fa_icon": "fas fa-terminal", "description": "Define parameters that control the stages in the pipeline", "properties": { + "skip_baserecalibration": { + "type": "boolean", + "description": "Skip the process of base recalibration steps i.e., GATK BaseRecalibrator and GATK ApplyBQSR.", + "help_text": "This parameter disable the base recalibration step, thus using a un-calibrated BAM file for variant calling." + }, "skip_intervallisttools": { "type": "boolean", "description": "Skip the process of preparing interval lists for the GATK variant calling step", diff --git a/tests/test_skipbasecalib.yml b/tests/test_skipbasecalib.yml new file mode 100644 index 00000000..7b5b2132 --- /dev/null +++ b/tests/test_skipbasecalib.yml @@ -0,0 +1,11 @@ +- name: Run pipeline without base calibration step + command: nextflow run main.nf -profile test,docker --skip_baserecalibration true + tags: + - skipbasecalib + - preprocessing + files: + - path: results/reports/multiqc_report.html + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz.tbi diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 89a5ee11..67238a82 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -90,6 +90,10 @@ def prepareToolIndices = params.aligner def seq_platform = params.seq_platform ? params.seq_platform : [] def seq_center = params.seq_center ? params.seq_center : [] +// Initialize file channels based on params +dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : [] +dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : [] + // Initialize varaint annotation associated channels def snpeff_db = params.snpeff_db ?: Channel.empty() def vep_cache_version = params.vep_cache_version ?: Channel.empty() @@ -229,55 +233,60 @@ workflow RNAVAR { splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) - // MODULE: BaseRecalibrator from GATK4 - ch_bqsr_table = Channel.empty() - known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() - known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() + bam_variant_calling = Channel.empty() - ch_interval_list_recalib = ch_interval_list.map{ meta, bed -> [bed] }.flatten() - splitncigar_bam_bai.combine(ch_interval_list_recalib) - .map{ meta, bam, bai, interval -> [ meta, bam, bai, interval] - }.set{splitncigar_bam_bai_interval} + if(!params.skip_baserecalibration) { + // MODULE: BaseRecalibrator from GATK4 + ch_bqsr_table = Channel.empty() + known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() + known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() - GATK4_BASERECALIBRATOR( - splitncigar_bam_bai_interval, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.dict, - known_sites, - known_sites_tbi - ) - ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table - ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) - - // MODULE: ApplyBaseRecalibrator from GATK4 - bam_applybqsr = splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) - bam_recalibrated = Channel.empty() - bam_recalibrated_qc = Channel.empty() - - ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.flatten() - bam_applybqsr.combine(ch_interval_list_applybqsr) - .map{ meta, bam, bai, table, interval -> [ meta, bam, bai, table, interval] - }.set{applybqsr_bam_bai_interval} - - RECALIBRATE( - ('bamqc' in params.skip_qc), - ('samtools' in params.skip_qc), - applybqsr_bam_bai_interval, - PREPARE_GENOME.out.dict, - PREPARE_GENOME.out.fai, - PREPARE_GENOME.out.fasta - ) + ch_interval_list_recalib = ch_interval_list.map{ meta, bed -> [bed] }.flatten() + splitncigar_bam_bai.combine(ch_interval_list_recalib) + .map{ meta, bam, bai, interval -> [ meta, bam, bai, interval] + }.set{splitncigar_bam_bai_interval} - bam_recalibrated = RECALIBRATE.out.bam - bam_recalibrated_qc = RECALIBRATE.out.qc - ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) + GATK4_BASERECALIBRATOR( + splitncigar_bam_bai_interval, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + known_sites, + known_sites_tbi + ) + ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) + + // MODULE: ApplyBaseRecalibrator from GATK4 + bam_applybqsr = splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) + bam_recalibrated_qc = Channel.empty() + + ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.flatten() + bam_applybqsr.combine(ch_interval_list_applybqsr) + .map{ meta, bam, bai, table, interval -> [ meta, bam, bai, table, interval] + }.set{applybqsr_bam_bai_interval} + + RECALIBRATE( + ('bamqc' in params.skip_qc), + ('samtools' in params.skip_qc), + applybqsr_bam_bai_interval, + PREPARE_GENOME.out.dict, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.fasta + ) + + bam_variant_calling = RECALIBRATE.out.bam + bam_recalibrated_qc = RECALIBRATE.out.qc + ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) + } else { + bam_variant_calling = splitncigar_bam_bai + } // MODULE: HaplotypeCaller from GATK4 interval_flag = params.no_intervals haplotypecaller_vcf = Channel.empty() - haplotypecaller_interval_bam = bam_recalibrated.combine(ch_interval_list_split) + haplotypecaller_interval_bam = bam_variant_calling.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() new_meta.id = meta.id @@ -286,8 +295,8 @@ workflow RNAVAR { GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, - params.dbsnp, - params.dbsnp_tbi, + dbsnp, + dbsnp_tbi, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, From 6eab34dcea0c535f2964a5f3a730f1a6ab7adda7 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 03:57:26 +0100 Subject: [PATCH 121/235] added: doc on skip_baserecalibration --- docs/output.md | 2 ++ docs/usage.md | 2 ++ 2 files changed, 4 insertions(+) diff --git a/docs/output.md b/docs/output.md index c831811f..77944f68 100644 --- a/docs/output.md +++ b/docs/output.md @@ -118,6 +118,8 @@ Currently, the pipeline does not produce the new BAM file in the output director ### Base (Quality Score) Recalibration +Base quality recalibration step runs by default and it produces the recalibrated BAM file for variant calling, as described below. However, you can turn off this step by using `--skip_baserecalibration true` option, and in that case, no recalibrated BAM file is produced and the pipeline uses the un-calibrated BAM file will be used for variant calling. + #### GATK BaseRecalibrator [GATK BaseRecalibrator](https://gatk.broadinstitute.org/hc/en-us/articles/360042477672-BaseRecalibrator) generates a recalibration table based on various co-variates. diff --git a/docs/usage.md b/docs/usage.md index b17c7ce7..a0fc87a8 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -122,6 +122,8 @@ Marking duplicate reads is performed using `Picard MarkDuplicates` tool. The too GATK best practices has been followed in this pipeline for RNA analysis, hence it uses GATK modules such as `SplitNCigarReads`, `BaseRecalibrator`, `ApplyBQSR`. The `BaseRecalibrator` process requires known variants sites VCF. ExAc, gnomAD, or dbSNP resources can be used as known sites of variation.You can supply the VCF and index files using parameters such as `--dbsnp`, `--dbsnp_tbi`, `--known_indels`, `--known_indels_tbi`. +> **NB:** Base recalibration can be turned off using `--skip_baserecalibration true` option. This is useful when you are analyzing data from non-model organisms where there is no known variant datasets exist. + `GATK SplitNCigarReads` is very time consuming step, therefore we made an attempt to break the GTF file into multiple chunks (scatters) using `GATK IntervalListTools` to run the process independently on each chunk in a parallel way to speed up the analysis. The default number of splits is set to 25, that means the GTF file is split into 25 smaller files and run `GATK SplitNCigarReads` on each of them in parallel. You can modify the number of splits using parameter `--scatter_count`. ## Variant calling and filtering From fa94c886d3f03c36f6154ecce9a1c22360e8eeaa Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 04:22:05 +0100 Subject: [PATCH 122/235] fix: markdownlint --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index a0fc87a8..a3c4b4bc 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -278,7 +278,7 @@ Work dir: Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` ``` -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ```nextflow process { From 89892fece48f014db571f88769b50a37137f2a2c Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 04:31:06 +0100 Subject: [PATCH 123/235] fix: markdownlint - attempt 2 --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 3b448773..950cc9f3 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,7 +14,7 @@ jobs: - uses: actions/checkout@v2 - uses: actions/setup-node@v1 with: - node-version: '10' + node-version: '12' - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint From df16faa8a38d9d933e962be8a7704b13803750a5 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 04:36:49 +0100 Subject: [PATCH 124/235] fix: markdownlint - revert --- .github/workflows/linting.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 950cc9f3..3b448773 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,7 +14,7 @@ jobs: - uses: actions/checkout@v2 - uses: actions/setup-node@v1 with: - node-version: '12' + node-version: '10' - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint From e1f7e02541cd9f79c97dbfa438f279b987378359 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 3 Feb 2022 08:45:53 +0100 Subject: [PATCH 125/235] fix: nodeversion in linting.yml --- .github/workflows/linting.yml | 4 +--- .nf-core.yml | 4 +++- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 3b448773..956cd90e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -12,9 +12,7 @@ jobs: runs-on: ubuntu-latest steps: - uses: actions/checkout@v2 - - uses: actions/setup-node@v1 - with: - node-version: '10' + - uses: actions/setup-node@v2 - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint diff --git a/.nf-core.yml b/.nf-core.yml index 8b137891..ebde8935 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,3 @@ - +lint: + files_unchanged: + - .github/workflows/linting.yml From c41d9057a71172747ff5235315a7bd3bdb7ceb2e Mon Sep 17 00:00:00 2001 From: m3hdad Date: Wed, 9 Feb 2022 09:57:05 +0100 Subject: [PATCH 126/235] gffread ext.args defined --- conf/modules.config | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index 7c2952ca..28a9c68a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -77,6 +77,16 @@ process { ] } + withName: GFFREAD { + ext.args = '--keep-exon-attrs -F -T' + publishDir = [ + path: { "${params.outdir}/genome" }, + mode: 'copy', + saveAs: { filename -> filename.equals('versions.yml') ?null : filename }, + enabled: params.save_reference + ] + } + withName: GTF2BED { publishDir = [ path: { "${params.outdir}/genome" }, From 95d3db0558b2cb1306dec102c00fb4e914826fd8 Mon Sep 17 00:00:00 2001 From: m3hdad Date: Wed, 9 Feb 2022 10:24:08 +0100 Subject: [PATCH 127/235] updating mandatory params to accept gff --- workflows/rnavar.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 89a5ee11..1637f890 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -16,6 +16,7 @@ def checkPathParamList = [ params.fasta_fai, params.dict, params.gtf, + params.gff, params.dbsnp, params.dbsnp_tbi, params.known_indels, @@ -28,7 +29,7 @@ for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } -if(!params.star_index and !params.gtf){ exit 1, "GTF file is required to build a STAR reference index! Use option -gtf to provide a GTF file." } +if(!params.star_index && !params.gtf && !params.gff){ exit 1, "GTF|GFF3 file is required to build a STAR reference index! Use option -gtf|-gff to provide a GTF|GFF file." } /* ======================================================================================== From 31d015213855daa58ac6143b1a3477b1a703e3b5 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 05:40:14 +0100 Subject: [PATCH 128/235] changes related to template merge 2 --- docs/output.md | 26 ------ docs/usage.md | 80 ------------------- modules.json | 8 +- .../custom/dumpsoftwareversions/main.nf | 3 - .../custom/dumpsoftwareversions/meta.yml | 4 - modules/nf-core/modules/fastqc/main.nf | 3 - modules/nf-core/modules/fastqc/meta.yml | 33 -------- modules/nf-core/modules/multiqc/main.nf | 10 --- modules/nf-core/modules/multiqc/meta.yml | 29 ------- workflows/rnavar.nf | 10 --- 10 files changed, 4 insertions(+), 202 deletions(-) diff --git a/docs/output.md b/docs/output.md index c3cfe2dd..77944f68 100644 --- a/docs/output.md +++ b/docs/output.md @@ -33,11 +33,6 @@ It used an institutional profile `munin` from [nf-core/configs](https://github.c The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -<<<<<<< HEAD -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -======= * [Preprocessing](#preprocessing) * [cat](#cat) - Merge re-sequenced FastQ files * [FastQC](#fastqc) - Raw read QC @@ -56,7 +51,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * [Workflow reporting and genomes](#workflow-reporting-and-genomes) * [Reference genome files](#reference-genome-files) - Saving reference genome indices/files * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ->>>>>>> upstream/dev ## Preprocessing @@ -65,14 +59,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
Output files -<<<<<<< HEAD -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -======= * `fastq/` * `*.merged.fastq.gz`: If `--save_merged_fastq` is specified, concatenated FastQ files will be placed in this directory. ->>>>>>> upstream/dev
@@ -87,12 +75,6 @@ If multiple libraries/runs have been provided for the same sample in the input s
Output files -<<<<<<< HEAD -- `multiqc/` - - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - - `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - - `multiqc_plots/`: directory containing static images from the report in various formats. -======= * `preprocessing/[SAMPLE]/` * `[SAMPLE].aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. * `[SAMPLE].aligned.bam.bai`: This is the index of the above *.aligned.bam @@ -266,7 +248,6 @@ The pipeline has special steps which also allow the software versions to be repo * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. * `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. * `multiqc_plots/`: directory containing static images from the report in various formats. ->>>>>>> upstream/dev
@@ -275,17 +256,10 @@ The pipeline has special steps which also allow the software versions to be repo
Output files -<<<<<<< HEAD -- `pipeline_info/` - - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. -======= * `pipeline_info/` * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. ->>>>>>> upstream/dev
diff --git a/docs/usage.md b/docs/usage.md index f5abaa33..1c1a78df 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -4,68 +4,12 @@ > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ -<<<<<<< HEAD -## Introduction - - - -## Samplesheet input - -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. - -```console ---input '[path to samplesheet file]' -``` - -### Multiple runs of the same sample - -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: - -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` - -### Full samplesheet - -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. - -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. - -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, -``` - -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | - -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. - -## Running the pipeline -======= ## RUNNING THE PIPELINE ->>>>>>> upstream/dev The typical command for running the pipeline is as follows: ```console -<<<<<<< HEAD -nextflow run nf-core/rnavar --input samplesheet.csv --outdir --genome GRCh37 -profile docker -======= nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile docker ->>>>>>> upstream/dev ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -306,19 +250,11 @@ Whilst the default requirements set within the pipeline will hopefully work for For example, if the nf-core/rnavar pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: ```console -<<<<<<< HEAD -[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' - -Caused by: - Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -======= [62/149eb0] NOTE: Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) Error executing process > 'RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)' Caused by: Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) ->>>>>>> upstream/dev Command executed: STAR \ @@ -342,17 +278,7 @@ Work dir: Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` ``` -<<<<<<< HEAD -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). -We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`. -If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). -The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. -The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. -Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. -The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. -======= To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ->>>>>>> upstream/dev ```nextflow process { @@ -362,11 +288,6 @@ process { } ``` -<<<<<<< HEAD -> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. -> -> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. -======= > **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNAVAR:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. ### Tool-specific options @@ -404,7 +325,6 @@ params { } } ``` ->>>>>>> upstream/dev ### Updating containers diff --git a/modules.json b/modules.json index c9c338e7..1ac9028d 100644 --- a/modules.json +++ b/modules.json @@ -7,13 +7,13 @@ "git_sha": "826a5603db5cf5b4f1e55cef9cc0b7c37d3c7e70" }, "custom/dumpsoftwareversions": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "ensemblvep": { "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" }, "fastqc": { - "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/applybqsr": { "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" @@ -46,7 +46,7 @@ "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" }, "multiqc": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "picard/markduplicates": { "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" @@ -89,4 +89,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf index dd8ca3a6..327d5100 100644 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf @@ -15,12 +15,9 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { path "software_versions_mqc.yml", emit: mqc_yml path "versions.yml" , emit: versions -<<<<<<< HEAD when: task.ext.when == null || task.ext.when -======= ->>>>>>> upstream/dev script: def args = task.ext.args ?: '' template 'dumpsoftwareversions.py' diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml index 268a3ba9..60b546a0 100644 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml @@ -8,11 +8,7 @@ tools: description: Custom module used to dump software versions within the nf-core pipeline template homepage: https://github.com/nf-core/tools documentation: https://github.com/nf-core/tools -<<<<<<< HEAD licence: ["MIT"] -======= - licence: ['MIT'] ->>>>>>> upstream/dev input: - versions: type: file diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf index 742275aa..ed6b8c50 100644 --- a/modules/nf-core/modules/fastqc/main.nf +++ b/modules/nf-core/modules/fastqc/main.nf @@ -14,12 +14,9 @@ process FASTQC { tuple val(meta), path("*.html"), emit: html tuple val(meta), path("*.zip") , emit: zip path "versions.yml" , emit: versions -<<<<<<< HEAD when: task.ext.when == null || task.ext.when -======= ->>>>>>> upstream/dev script: def args = task.ext.args ?: '' diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/modules/fastqc/meta.yml index 259394b5..4da5bb5a 100644 --- a/modules/nf-core/modules/fastqc/meta.yml +++ b/modules/nf-core/modules/fastqc/meta.yml @@ -6,7 +6,6 @@ keywords: - adapters - fastq tools: -<<<<<<< HEAD - fastqc: description: | FastQC gives general quality metrics about your reads. @@ -17,18 +16,6 @@ tools: homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ licence: ["GPL-2.0-only"] -======= - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ['GPL-2.0-only'] ->>>>>>> upstream/dev input: - meta: type: map @@ -41,7 +28,6 @@ input: List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. output: -<<<<<<< HEAD - meta: type: map description: | @@ -59,25 +45,6 @@ output: type: file description: File containing software versions pattern: "versions.yml" -======= - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" ->>>>>>> upstream/dev authors: - "@drpatelh" - "@grst" diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf index c7dfba02..1264aac1 100644 --- a/modules/nf-core/modules/multiqc/main.nf +++ b/modules/nf-core/modules/multiqc/main.nf @@ -1,17 +1,10 @@ process MULTIQC { label 'process_medium' -<<<<<<< HEAD conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' : 'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }" -======= - conda (params.enable_conda ? 'bioconda::multiqc=1.11' : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }" ->>>>>>> upstream/dev input: path multiqc_files @@ -21,12 +14,9 @@ process MULTIQC { path "*_data" , emit: data path "*_plots" , optional:true, emit: plots path "versions.yml" , emit: versions -<<<<<<< HEAD when: task.ext.when == null || task.ext.when -======= ->>>>>>> upstream/dev script: def args = task.ext.args ?: '' diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/modules/multiqc/meta.yml index 0e83244b..6fa891ef 100644 --- a/modules/nf-core/modules/multiqc/meta.yml +++ b/modules/nf-core/modules/multiqc/meta.yml @@ -5,7 +5,6 @@ keywords: - bioinformatics tools - Beautiful stand-alone HTML report tools: -<<<<<<< HEAD - multiqc: description: | MultiQC searches a given directory for analysis logs and compiles a HTML report. @@ -13,22 +12,12 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] -======= - - multiqc: - description: | - MultiQC searches a given directory for analysis logs and compiles a HTML report. - It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. - homepage: https://multiqc.info/ - documentation: https://multiqc.info/docs/ - licence: ['GPL-3.0-or-later'] ->>>>>>> upstream/dev input: - multiqc_files: type: file description: | List of reports / files recognised by MultiQC, for example the html and zip output of FastQC output: -<<<<<<< HEAD - report: type: file description: MultiQC report file @@ -45,24 +34,6 @@ output: type: file description: File containing software versions pattern: "versions.yml" -======= - - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" - - data: - type: dir - description: MultiQC data dir - pattern: "multiqc_data" - - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" ->>>>>>> upstream/dev authors: - "@abhi18av" - "@bunop" diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 414e7205..2e11a545 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -115,8 +115,6 @@ def multiqc_report = [] workflow RNAVAR { ch_versions = Channel.empty() -<<<<<<< HEAD -======= // // SUBWORKFLOW: Uncompress and prepare reference genome files @@ -124,7 +122,6 @@ workflow RNAVAR { PREPARE_GENOME (prepareToolIndices) ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) ->>>>>>> upstream/dev // // SUBWORKFLOW: Read in samplesheet, validate and stage input files @@ -132,9 +129,6 @@ workflow RNAVAR { INPUT_CHECK ( ch_input ) -<<<<<<< HEAD - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) -======= .reads .map { meta, fastq -> @@ -161,7 +155,6 @@ workflow RNAVAR { .mix(ch_fastq.single) .set { ch_cat_fastq } ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null)) ->>>>>>> upstream/dev // // MODULE: Run FastQC @@ -172,8 +165,6 @@ workflow RNAVAR { ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) -<<<<<<< HEAD -======= // // PREPARE THE INTERVAL LIST FROM GTF FILE // @@ -368,7 +359,6 @@ workflow RNAVAR { } ->>>>>>> upstream/dev CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From b6035b4787d859c7b03574b2ccdabaff23d49ad0 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 05:40:45 +0100 Subject: [PATCH 129/235] changes related to template merge #3 --- .github/PULL_REQUEST_TEMPLATE.md | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index ab5c01d7..01f4491e 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,6 +10,7 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnavar/tree/master/.github/CONTRIBUTING.md) --> + ## PR checklist From d973e9b005851495135c1bb528cd6f332b03ba09 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 07:56:06 +0100 Subject: [PATCH 130/235] fix: lint errors --- .nf-core.yml | 3 --- assets/samplesheet_test.csv | 2 +- bin/check_samplesheet.py | 2 ++ nextflow.config | 2 +- null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt | 1 + 5 files changed, 5 insertions(+), 5 deletions(-) create mode 100644 null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt diff --git a/.nf-core.yml b/.nf-core.yml index 43091995..3805dc81 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,4 +1 @@ repository_type: pipeline -lint: - files_unchanged: - - .github/workflows/linting.yml diff --git a/assets/samplesheet_test.csv b/assets/samplesheet_test.csv index 7131bd5d..125f9bfd 100644 --- a/assets/samplesheet_test.csv +++ b/assets/samplesheet_test.csv @@ -1,2 +1,2 @@ sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 5473b624..a69ea91d 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -97,6 +97,8 @@ def _validate_pair(self, row): """Assert that read pairs have the same file extension. Report pair status.""" if row[self._first_col] and row[self._second_col]: row[self._single_col] = False + print(Path(row[self._first_col]).suffixes) + print(Path(row[self._second_col]).suffixes) assert ( Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes ), "FASTQ pairs must have the same file extensions." diff --git a/nextflow.config b/nextflow.config index d2df55e0..52cf896e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -79,7 +79,7 @@ params { max_multiqc_email_size = '25.MB' // Boilerplate options - outdir = null + outdir = 'results' tracedir = "${params.outdir}/pipeline_info" publish_dir_mode = 'copy' email = null diff --git a/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt b/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt new file mode 100644 index 00000000..6b739acd --- /dev/null +++ b/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt @@ -0,0 +1 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar From 44215773b7dc6cf976023062cf5d5a6673a1f465 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 07:56:40 +0100 Subject: [PATCH 131/235] fix: lint errors using --fix files_unchanged --- .github/PULL_REQUEST_TEMPLATE.md | 1 - 1 file changed, 1 deletion(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 01f4491e..ab5c01d7 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -10,7 +10,6 @@ Remember that PRs should be made against the dev branch, unless you're preparing Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnavar/tree/master/.github/CONTRIBUTING.md) --> - ## PR checklist From c2085b5abb611ae38a7e19dad41f65cfc56e6390 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 08:27:54 +0100 Subject: [PATCH 132/235] Testing --- bin/check_samplesheet.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index a69ea91d..5473b624 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -97,8 +97,6 @@ def _validate_pair(self, row): """Assert that read pairs have the same file extension. Report pair status.""" if row[self._first_col] and row[self._second_col]: row[self._single_col] = False - print(Path(row[self._first_col]).suffixes) - print(Path(row[self._second_col]).suffixes) assert ( Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes ), "FASTQ pairs must have the same file extensions." From 6fc8aa733fe73231528d230141cd1a2eb3995f8d Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 08:31:53 +0100 Subject: [PATCH 133/235] Changed: fastq file name in samplesheet_test.csv --- tests/csv/samplesheet_test.csv | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/csv/samplesheet_test.csv b/tests/csv/samplesheet_test.csv index 81e928c9..d6a719d9 100644 --- a/tests/csv/samplesheet_test.csv +++ b/tests/csv/samplesheet_test.csv @@ -1,3 +1,3 @@ sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse -TEST123,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz,reverse +GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse +TEST123,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse From 54c2b79e4689e26d55a10d1bd8c379b091383707 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 14:38:59 +0100 Subject: [PATCH 134/235] Fix: error while getting sample name from meta --- bin/check_samplesheet.py | 2 +- workflows/rnavar.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 5473b624..d1a9919e 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -98,7 +98,7 @@ def _validate_pair(self, row): if row[self._first_col] and row[self._second_col]: row[self._single_col] = False assert ( - Path(row[self._first_col]).suffixes == Path(row[self._second_col]).suffixes + Path(row[self._first_col]).suffixes[-2:] == Path(row[self._second_col]).suffixes[-2:] ), "FASTQ pairs must have the same file extensions." else: row[self._single_col] = True diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 2e11a545..8c4a94a4 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -132,7 +132,7 @@ workflow RNAVAR { .reads .map { meta, fastq -> - meta.id = meta.id.split('_')[0..-2].join('_') + meta.id = meta.id.split('_')[0..meta.id.split('_').size()-2].join('_') [ meta, fastq ] } .groupTuple(by: [0]) .branch { From c5fb926f76ed007221ecee24e8d84f2321d2e7b4 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 15:34:42 +0100 Subject: [PATCH 135/235] fix: prettier warnings --- .github/workflows/ci.yml | 18 +-- README.md | 6 +- assets/multiqc_config.yaml | 14 +- docs/output.md | 152 +++++++++--------- docs/usage.md | 34 ++-- modules.json | 2 +- modules/local/gatk4/haplotypecaller/meta.yml | 6 +- modules/local/gatk4/splitncigarreads/meta.yml | 2 +- modules/nf-core/modules/cat/fastq/meta.yml | 62 +++---- modules/nf-core/modules/ensemblvep/meta.yml | 114 ++++++------- .../nf-core/modules/gatk4/applybqsr/meta.yml | 9 +- .../modules/gatk4/baserecalibrator/meta.yml | 9 +- .../modules/gatk4/bedtointervallist/meta.yml | 2 +- .../gatk4/createsequencedictionary/meta.yml | 48 +++--- .../modules/gatk4/indexfeaturefile/meta.yml | 2 +- .../modules/gatk4/intervallisttools/meta.yml | 2 +- .../nf-core/modules/gatk4/mergevcfs/meta.yml | 2 +- .../modules/gatk4/variantfiltration/meta.yml | 2 +- modules/nf-core/modules/gffread/meta.yml | 8 +- modules/nf-core/modules/gunzip/meta.yml | 54 +++---- .../modules/picard/markduplicates/meta.yml | 2 +- .../nf-core/modules/samtools/faidx/meta.yml | 72 ++++----- .../modules/samtools/flagstat/meta.yml | 84 +++++----- .../modules/samtools/idxstats/meta.yml | 86 +++++----- .../nf-core/modules/samtools/index/meta.yml | 92 +++++------ .../nf-core/modules/samtools/merge/meta.yml | 94 +++++------ .../nf-core/modules/samtools/sort/meta.yml | 74 ++++----- .../nf-core/modules/samtools/stats/meta.yml | 90 +++++------ modules/nf-core/modules/snpeff/meta.yml | 100 ++++++------ modules/nf-core/modules/star/align/meta.yml | 2 +- .../modules/star/genomegenerate/meta.yml | 2 +- .../nf-core/modules/tabix/bgziptabix/meta.yml | 76 ++++----- modules/nf-core/modules/untar/meta.yml | 42 ++--- nextflow_schema.json | 7 +- 34 files changed, 683 insertions(+), 688 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 0a1be4d5..1eb793b1 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,16 +23,16 @@ jobs: # Nextflow versions include: # Test pipeline minimum Nextflow version - - NXF_VER: '21.10.3' - NXF_EDGE: '' + - NXF_VER: "21.10.3" + NXF_EDGE: "" # Test latest edge release of Nextflow - - NXF_VER: '' - NXF_EDGE: '1' + - NXF_VER: "" + NXF_EDGE: "1" test: - - 'default' - - 'annotation' - - 'removeduplicates' - - 'skipbasecalib' + - "default" + - "annotation" + - "removeduplicates" + - "skipbasecalib" steps: - name: Check out pipeline code uses: actions/checkout@v2 @@ -50,7 +50,7 @@ jobs: - name: Set up Python uses: actions/setup-python@v2 with: - python-version: '3.x' + python-version: "3.x" - name: Install dependencies run: python -m pip install --upgrade pip pytest-workflow diff --git a/README.md b/README.md index 9d5eb14d..a8883ed7 100644 --- a/README.md +++ b/README.md @@ -60,9 +60,9 @@ On release, automated continuous integration tests run the pipeline on a full-si 4. Start running your own analysis! - ```console - nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh38 - ``` + ```console + nextflow run nf-core/rnavar -profile --input samplesheet.csv --genome GRCh38 + ``` ## Documentation diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml index 917528e8..746053ab 100644 --- a/assets/multiqc_config.yaml +++ b/assets/multiqc_config.yaml @@ -1,11 +1,11 @@ report_comment: > - This report has been generated by the nf-core/rnavar - analysis pipeline. For information about how to interpret these results, please see the - documentation. + This report has been generated by the nf-core/rnavar + analysis pipeline. For information about how to interpret these results, please see the + documentation. report_section_order: - software_versions: - order: -1000 - nf-core-rnavar-summary: - order: -1001 + software_versions: + order: -1000 + nf-core-rnavar-summary: + order: -1001 export_plots: true diff --git a/docs/output.md b/docs/output.md index 77944f68..35c2915c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -33,24 +33,24 @@ It used an institutional profile `munin` from [nf-core/configs](https://github.c The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [Preprocessing](#preprocessing) - * [cat](#cat) - Merge re-sequenced FastQ files - * [FastQC](#fastqc) - Raw read QC -* [Alignment](#alignment) - * [STAR](#star) - Fast spliced aware genome alignment -* [Alignment post-processing](#alignment-post-processing) - * [Picard MarkDuplicates](#picard-markduplicates) - Duplicate read marking - * [GATK4 SplitNCigarReads](#gatk4-splitncigarreads) - Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data) - * [GATK4 Base Quality Score Recalibration (BQSR)](#gatk4-bqsr) - Estimate and correct systematic bias that affect the assignment of base quality scores by the sequencer -* [Variant calling](#other-steps) - * [GATK4 HaplotypeCaller](#gatk4-haplotypecaller) - Call SNPs and indels via local re-assembly of haplotypes -* [Variant filtering](#other-steps) - * [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria -* [Quality control](#quality-control) - * [MultiQC](#multiqc) - Present QC for raw reads, alignment, read counting and sample similiarity -* [Workflow reporting and genomes](#workflow-reporting-and-genomes) - * [Reference genome files](#reference-genome-files) - Saving reference genome indices/files - * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution +- [Preprocessing](#preprocessing) + - [cat](#cat) - Merge re-sequenced FastQ files + - [FastQC](#fastqc) - Raw read QC +- [Alignment](#alignment) + - [STAR](#star) - Fast spliced aware genome alignment +- [Alignment post-processing](#alignment-post-processing) + - [Picard MarkDuplicates](#picard-markduplicates) - Duplicate read marking + - [GATK4 SplitNCigarReads](#gatk4-splitncigarreads) - Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data) + - [GATK4 Base Quality Score Recalibration (BQSR)](#gatk4-bqsr) - Estimate and correct systematic bias that affect the assignment of base quality scores by the sequencer +- [Variant calling](#other-steps) + - [GATK4 HaplotypeCaller](#gatk4-haplotypecaller) - Call SNPs and indels via local re-assembly of haplotypes +- [Variant filtering](#other-steps) + - [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria +- [Quality control](#quality-control) + - [MultiQC](#multiqc) - Present QC for raw reads, alignment, read counting and sample similiarity +- [Workflow reporting and genomes](#workflow-reporting-and-genomes) + - [Reference genome files](#reference-genome-files) - Saving reference genome indices/files + - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ## Preprocessing @@ -59,8 +59,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
Output files -* `fastq/` - * `*.merged.fastq.gz`: If `--save_merged_fastq` is specified, concatenated FastQ files will be placed in this directory. +- `fastq/` + - `*.merged.fastq.gz`: If `--save_merged_fastq` is specified, concatenated FastQ files will be placed in this directory.
@@ -75,18 +75,18 @@ If multiple libraries/runs have been provided for the same sample in the input s
Output files -* `preprocessing/[SAMPLE]/` - * `[SAMPLE].aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. - * `[SAMPLE].aligned.bam.bai`: This is the index of the above *.aligned.bam -* `preprocessing/[SAMPLE]/log` - * `[SAMPLE].Log.final.out`: STAR alignment report containing the mapping results summary. - * `[SAMPLE].Log.out` and `[SAMPLE].Log.progress.out`: STAR log files containing detailed information about the run. Typically only useful for debugging purposes. - * `[SAMPLE].SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. -* `preprocessing/[SAMPLE]/unmapped` - * `[SAMPLE].unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. -* `reports/samtools_stats/[SAMPLE]/` - * `[SAMPLE].aligned.bam.flagstat`: Samtools flagstat summary of the alignment - * `[SAMPLE].aligned.bam.stats`: Samtools stat output +- `preprocessing/[SAMPLE]/` + - `[SAMPLE].aligned.bam`: If `--save_align_intermeds` is specified the original BAM file containing read alignments to the reference genome will be placed in this directory. + - `[SAMPLE].aligned.bam.bai`: This is the index of the above \*.aligned.bam +- `preprocessing/[SAMPLE]/log` + - `[SAMPLE].Log.final.out`: STAR alignment report containing the mapping results summary. + - `[SAMPLE].Log.out` and `[SAMPLE].Log.progress.out`: STAR log files containing detailed information about the run. Typically only useful for debugging purposes. + - `[SAMPLE].SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. +- `preprocessing/[SAMPLE]/unmapped` + - `[SAMPLE].unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. +- `reports/samtools_stats/[SAMPLE]/` + - `[SAMPLE].aligned.bam.flagstat`: Samtools flagstat summary of the alignment + - `[SAMPLE].aligned.bam.stats`: Samtools stat output
@@ -101,18 +101,18 @@ If desired, duplicates can be removed using the `--remove_duplicates true` optio
Output files -* `preprocessing/[SAMPLE]/` - * `[SAMPLE].markdup.sorted.bam`: Picard Markduplicate bam file. - * `[SAMPLE].markdup.sorted.bam.bai`: This is the index of the above *.aligned.bam -* `reports/samtools_stats/[SAMPLE]/` - * `[SAMPLE].markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment - * `[SAMPLE].markdup.sorted.bam.stats`: Samtools stat output +- `preprocessing/[SAMPLE]/` + - `[SAMPLE].markdup.sorted.bam`: Picard Markduplicate bam file. + - `[SAMPLE].markdup.sorted.bam.bai`: This is the index of the above \*.aligned.bam +- `reports/samtools_stats/[SAMPLE]/` + - `[SAMPLE].markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment + - `[SAMPLE].markdup.sorted.bam.stats`: Samtools stat output
### SplitNCigarReads -[GATK SplitNCigarReads](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads) is executed for post-processing RNA reads aligned against the reference. The tool's main output is a new BAM file with reads split at N CIGAR elements and CIGAR strings updated. +[GATK SplitNCigarReads](https://gatk.broadinstitute.org/hc/en-us/articles/360036858811-SplitNCigarReads) is executed for post-processing RNA reads aligned against the reference. The tool's main output is a new BAM file with reads split at N CIGAR elements and CIGAR strings updated. Currently, the pipeline does not produce the new BAM file in the output directory. @@ -133,9 +133,9 @@ Currently, the pipeline does not produce the recalibration table file in the out
Output files -* `preprocessing/[SAMPLE]/` - * `[SAMPLE].recal.bam`: Recalibrated bam file. - * `[SAMPLE].recal.bam.bai`: This is the index of the above recalibrated bam. +- `preprocessing/[SAMPLE]/` + - `[SAMPLE].recal.bam`: Recalibrated bam file. + - `[SAMPLE].recal.bam.bai`: This is the index of the above recalibrated bam.
@@ -146,9 +146,9 @@ Currently, the pipeline does not produce the recalibration table file in the out
Output files -* `results/variant_calling/[SAMPLE]/` - * `[SAMPLE].haplotypecaller.vcf.gz`: Variant calls in VCF format. - * `[SAMPLE].haplotypecaller.vcf.gz.tbi`: This is the index of the above VCF file. +- `results/variant_calling/[SAMPLE]/` + - `[SAMPLE].haplotypecaller.vcf.gz`: Variant calls in VCF format. + - `[SAMPLE].haplotypecaller.vcf.gz.tbi`: This is the index of the above VCF file.
@@ -159,9 +159,9 @@ Currently, the pipeline does not produce the recalibration table file in the out
Output files -* `results/variant_calling/[SAMPLE]/` - * `[SAMPLE].haplotypecaller.filtered.vcf.gz`: Variant VCF with updated FILTER field. - * `[SAMPLE].haplotypecaller.filtered.vcf.gz.tbi`: This is the index of the above VCF file. +- `results/variant_calling/[SAMPLE]/` + - `[SAMPLE].haplotypecaller.filtered.vcf.gz`: Variant VCF with updated FILTER field. + - `[SAMPLE].haplotypecaller.filtered.vcf.gz.tbi`: This is the index of the above VCF file.
@@ -181,9 +181,9 @@ The annotated variant files in VCF format can be found in `results/variant_annot
Output files -* `results/variant_annotation/[SAMPLE]/` - * `[SAMPLE]_snpEff.ann.vcf.gz`: Annotated VCF from snpEff process. - * `[SAMPLE]_snpEff.ann.vcf.gz.tbi`: This is the index of the above VCF file. +- `results/variant_annotation/[SAMPLE]/` + - `[SAMPLE]_snpEff.ann.vcf.gz`: Annotated VCF from snpEff process. + - `[SAMPLE]_snpEff.ann.vcf.gz.tbi`: This is the index of the above VCF file.
@@ -196,17 +196,17 @@ The format of the [consequence annotations](https://www.ensembl.org/info/genome/ Currently, it contains: -* `Consequence`: impact of the variation, if there is any -* `Codons`: the codon change, i.e. cGt/cAt -* `Amino_acids`: change in amino acids, i.e. R/H if there is any -* `Gene`: ENSEMBL gene name -* `SYMBOL`: gene symbol -* `Feature`: actual transcript name -* `EXON`: affected exon -* `PolyPhen`: prediction based on [PolyPhen](http://genetics.bwh.harvard.edu/pph2/) -* `SIFT`: prediction by [SIFT](http://sift.bii.a-star.edu.sg/) -* `Protein_position`: Relative position of amino acid in protein -* `BIOTYPE`: Biotype of transcript or regulatory feature +- `Consequence`: impact of the variation, if there is any +- `Codons`: the codon change, i.e. cGt/cAt +- `Amino_acids`: change in amino acids, i.e. R/H if there is any +- `Gene`: ENSEMBL gene name +- `SYMBOL`: gene symbol +- `Feature`: actual transcript name +- `EXON`: affected exon +- `PolyPhen`: prediction based on [PolyPhen](http://genetics.bwh.harvard.edu/pph2/) +- `SIFT`: prediction by [SIFT](http://sift.bii.a-star.edu.sg/) +- `Protein_position`: Relative position of amino acid in protein +- `BIOTYPE`: Biotype of transcript or regulatory feature To annotate variants using `vep`, you can use `--annotate_tools vep`. The annotated variant files in VCF format can be found in `results/variant_annotation` folder. @@ -214,9 +214,9 @@ The annotated variant files in VCF format can be found in `results/variant_annot
Output files -* `results/variant_annotation/[SAMPLE]/` - * `[SAMPLE]_VEP.ann.vcf.gz`: Annotated VCF from VEP process. - * `[SAMPLE]_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file. +- `results/variant_annotation/[SAMPLE]/` + - `[SAMPLE]_VEP.ann.vcf.gz`: Annotated VCF from VEP process. + - `[SAMPLE]_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file.
@@ -225,9 +225,9 @@ When `--annotate_tools merge` option is used, the annotation from both `snpeff`
Output files -* `results/variant_annotation/[SAMPLE]/` - * `[SAMPLE]_snpEff_VEP.ann.vcf.gz`: Combined annotation from both snpEff and VEP. - * `[SAMPLE]_snpEff_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file. +- `results/variant_annotation/[SAMPLE]/` + - `[SAMPLE]_snpEff_VEP.ann.vcf.gz`: Combined annotation from both snpEff and VEP. + - `[SAMPLE]_snpEff_VEP.ann.vcf.gz.tbi`: This is the index of the above VCF file.
@@ -244,10 +244,10 @@ The pipeline has special steps which also allow the software versions to be repo
Output files -* `reports/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. +- `reports/` + - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + - `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. + - `multiqc_plots/`: directory containing static images from the report in various formats.
@@ -256,10 +256,10 @@ The pipeline has special steps which also allow the software versions to be repo
Output files -* `pipeline_info/` - * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. +- `pipeline_info/` + - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. + - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`.
diff --git a/docs/usage.md b/docs/usage.md index 1c1a78df..d35bb8ef 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -76,7 +76,7 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,reverse ``` | Column | Description | -|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| +| -------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | | `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | @@ -98,10 +98,10 @@ The index building step can be quite a time-consuming process and it permits the > **NB:** If you are supplying a pre-built genome index file via `--star_index`, please ensure that the index has been generated with the latest STAR version i.e. v2.7.9a or above. In case if the pipeline found an incompatible index, it will generate a new one using the reference genome which will consume time and memory unnecessarily. -* If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. -* If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. -* If `--gene_bed` is not provided then it will be generated from the GTF file. -* If `--star_index` is not provided then it will be generated from the reference genome FASTA file using `STAR --runmode genomeGenerate` command. +- If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. +- If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. +- If `--gene_bed` is not provided then it will be generated from the GTF file. +- If `--star_index` is not provided then it will be generated from the reference genome FASTA file using `STAR --runmode genomeGenerate` command. > **NB:** In case if you are providing a GTF and/or a BED file, please ensure that the chromosomes and contigs in the files are also present in the genome FASTA (and in the .dict) file. Otherwise `GATK BedToIntervalList` module is likely to fail if the chromosomes/contigs do not match with the reference genome data. @@ -132,10 +132,10 @@ GATK best practices has been followed in this pipeline for RNA analysis, hence i The pipeline runs a hard-filtering step on the variants by default. It does not filter out any variants, rather it flags i.e. PASS or other flags such as FS, QD, SnpCluster, etc. in FILTER column of the VCF. The following are the default filter criteria, however it can be changed using the respective parameters. -* `--cluster` is set to 3. It is the number of SNPs which make up a cluster. -* `--window` is set to 35. The window size (in bases) in which to evaluate clustered SNPs. -* `--fs_filter` is set to 30.0. Filter based on FisherStrand > 30.0. It is the Phred-scaled probability that there is strand bias at the site. -* `--qd_filter` is set to 2.0 meaning filter variants if Quality By Depth filter is < 2.0. +- `--cluster` is set to 3. It is the number of SNPs which make up a cluster. +- `--window` is set to 35. The window size (in bases) in which to evaluate clustered SNPs. +- `--fs_filter` is set to 30.0. Filter based on FisherStrand > 30.0. It is the Phred-scaled probability that there is strand bias at the site. +- `--qd_filter` is set to 2.0 meaning filter variants if Quality By Depth filter is < 2.0. Variant filtering is an optional step. You can skip it using `--skip_variantfiltration` parameter. @@ -173,9 +173,9 @@ nextflow run download_cache.nf --vep_cache --species Date: Tue, 22 Mar 2022 15:41:12 +0100 Subject: [PATCH 136/235] fix: template merge; module updates --- modules.json | 54 +++++++++---------- modules/nf-core/modules/cat/fastq/main.nf | 3 ++ modules/nf-core/modules/ensemblvep/main.nf | 3 ++ modules/nf-core/modules/fastqc/main.nf | 12 +++++ .../nf-core/modules/gatk4/applybqsr/main.nf | 9 ++-- .../modules/gatk4/baserecalibrator/main.nf | 9 ++-- .../modules/gatk4/bedtointervallist/main.nf | 20 +++++-- .../gatk4/createsequencedictionary/main.nf | 20 +++++-- .../gatk4/createsequencedictionary/meta.yml | 34 ++++++------ .../modules/gatk4/indexfeaturefile/main.nf | 9 ++-- .../modules/gatk4/intervallisttools/main.nf | 27 ++++++++-- .../nf-core/modules/gatk4/mergevcfs/main.nf | 9 ++-- .../modules/gatk4/variantfiltration/main.nf | 9 ++-- modules/nf-core/modules/gffread/main.nf | 3 ++ modules/nf-core/modules/gunzip/main.nf | 3 ++ modules/nf-core/modules/gunzip/meta.yml | 34 ++++++------ modules/nf-core/modules/multiqc/main.nf | 12 +++++ .../modules/picard/markduplicates/main.nf | 16 ++++++ .../nf-core/modules/samtools/faidx/main.nf | 19 +++++-- .../nf-core/modules/samtools/flagstat/main.nf | 9 ++-- .../nf-core/modules/samtools/idxstats/main.nf | 9 ++-- .../nf-core/modules/samtools/index/main.nf | 21 ++++++-- .../nf-core/modules/samtools/merge/main.nf | 21 ++++++-- modules/nf-core/modules/samtools/sort/main.nf | 20 +++++-- .../nf-core/modules/samtools/stats/main.nf | 20 +++++-- modules/nf-core/modules/snpeff/main.nf | 3 ++ modules/nf-core/modules/star/align/main.nf | 4 ++ .../modules/star/genomegenerate/main.nf | 3 ++ .../nf-core/modules/tabix/bgziptabix/main.nf | 17 +++++- modules/nf-core/modules/untar/main.nf | 26 ++++++--- modules/nf-core/modules/untar/meta.yml | 10 ++++ 31 files changed, 355 insertions(+), 113 deletions(-) diff --git a/modules.json b/modules.json index 2a017d48..58e0d630 100644 --- a/modules.json +++ b/modules.json @@ -4,88 +4,88 @@ "repos": { "nf-core/modules": { "cat/fastq": { - "git_sha": "826a5603db5cf5b4f1e55cef9cc0b7c37d3c7e70" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "custom/dumpsoftwareversions": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "ensemblvep": { - "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "fastqc": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/applybqsr": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/baserecalibrator": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/bedtointervallist": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/createsequencedictionary": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/indexfeaturefile": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/intervallisttools": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/mergevcfs": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/variantfiltration": { - "git_sha": "598d7abdb2a8df1aa3471c48d9186a9e3465983f" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gffread": { - "git_sha": "d473a247d2e0c619b0df877ea19d9a5a98c8e3c8" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gunzip": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "multiqc": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "picard/markduplicates": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/faidx": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/flagstat": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35" }, "samtools/idxstats": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35" }, "samtools/index": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/merge": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/sort": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/stats": { - "git_sha": "e751e5040af57e1b4e06ed4e0f3efe6de25c1683" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "snpeff": { - "git_sha": "3b366c7c6aac446c1a4ea7c2016092344633b2ec" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "star/align": { - "git_sha": "9d0cad583b9a71a6509b754fdf589cbfbed08961" + "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614" }, "star/genomegenerate": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "tabix/bgziptabix": { - "git_sha": "e22966ce74340cb671576143e5fdbbd71670cffa" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "untar": { - "git_sha": "20d8250d9f39ddb05dfb437603aaf99b5c0b2b41" + "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" } } } diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf index d02598e1..bf0877c3 100644 --- a/modules/nf-core/modules/cat/fastq/main.nf +++ b/modules/nf-core/modules/cat/fastq/main.nf @@ -14,6 +14,9 @@ process CAT_FASTQ { tuple val(meta), path("*.merged.fastq.gz"), emit: reads path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/ensemblvep/main.nf b/modules/nf-core/modules/ensemblvep/main.nf index e3d0c286..c2bd055f 100644 --- a/modules/nf-core/modules/ensemblvep/main.nf +++ b/modules/nf-core/modules/ensemblvep/main.nf @@ -19,6 +19,9 @@ process ENSEMBLVEP { path "*.summary.html" , emit: report path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/modules/fastqc/main.nf index ed6b8c50..05730368 100644 --- a/modules/nf-core/modules/fastqc/main.nf +++ b/modules/nf-core/modules/fastqc/main.nf @@ -44,4 +44,16 @@ process FASTQC { END_VERSIONS """ } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.html + touch ${prefix}.zip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index c246190a..851afc04 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -2,10 +2,10 @@ process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals) @@ -18,6 +18,9 @@ process GATK4_APPLYBQSR { tuple val(meta), path("*.cram"), emit: cram, optional: true path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index 42391d62..ecb41d9b 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -2,10 +2,10 @@ process GATK4_BASERECALIBRATOR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) @@ -19,6 +19,9 @@ process GATK4_BASERECALIBRATOR { tuple val(meta), path("*.table"), emit: table path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index 37f46f48..c3b624a8 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -2,10 +2,10 @@ process GATK4_BEDTOINTERVALLIST { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(bed) @@ -15,6 +15,9 @@ process GATK4_BEDTOINTERVALLIST { tuple val(meta), path('*.interval_list'), emit: interval_list path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" @@ -36,4 +39,15 @@ process GATK4_BEDTOINTERVALLIST { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.interval_list + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index 2cbd8948..fe1a6c65 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -2,10 +2,10 @@ process GATK4_CREATESEQUENCEDICTIONARY { tag "$fasta" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: path fasta @@ -14,6 +14,9 @@ process GATK4_CREATESEQUENCEDICTIONARY { path "*.dict" , emit: dict path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def avail_mem = 6 @@ -34,4 +37,15 @@ process GATK4_CREATESEQUENCEDICTIONARY { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.dict + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml index 69c23581..bd247888 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/meta.yml @@ -5,28 +5,28 @@ keywords: - fasta tools: - gatk: - description: | - Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools - with a primary focus on variant discovery and genotyping. Its powerful processing engine - and high-performance computing features make it capable of taking on projects of any size. - homepage: https://gatk.broadinstitute.org/hc/en-us - documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s - doi: 10.1158/1538-7445.AM2017-3590 - licence: ["Apache-2.0"] + description: | + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s + doi: 10.1158/1538-7445.AM2017-3590 + licence: ["Apache-2.0"] input: - fasta: - type: file - description: Input fasta file - pattern: "*.{fasta,fa}" + type: file + description: Input fasta file + pattern: "*.{fasta,fa}" output: - dict: - type: file - description: gatk dictionary file - pattern: "*.{dict}" + type: file + description: gatk dictionary file + pattern: "*.{dict}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@maxulysse" diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index ecd24173..275e51f5 100644 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -2,10 +2,10 @@ process GATK4_INDEXFEATUREFILE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(feature_file) @@ -14,6 +14,9 @@ process GATK4_INDEXFEATUREFILE { tuple val(meta), path("*.{tbi,idx}"), emit: index path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def avail_mem = 3 diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index 17412160..82c3222c 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -2,10 +2,10 @@ process GATK4_INTERVALLISTTOOLS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(interval_list) @@ -14,6 +14,9 @@ process GATK4_INTERVALLISTTOOLS { tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" @@ -48,4 +51,22 @@ process GATK4_INTERVALLISTTOOLS { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir -p ${prefix}_split/temp_0001_of_6 + mkdir -p ${prefix}_split/temp_0002_of_6 + mkdir -p ${prefix}_split/temp_0003_of_6 + mkdir -p ${prefix}_split/temp_0004_of_6 + touch ${prefix}_split/temp_0001_of_6/1scattered.interval_list + touch ${prefix}_split/temp_0002_of_6/2scattered.interval_list + touch ${prefix}_split/temp_0003_of_6/3scattered.interval_list + touch ${prefix}_split/temp_0004_of_6/4scattered.interval_list + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf index b2e584a8..54e38667 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -2,10 +2,10 @@ process GATK4_MERGEVCFS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(vcfs) @@ -16,6 +16,9 @@ process GATK4_MERGEVCFS { tuple val(meta), path('*.vcf.gz'), emit: vcf path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index beaec0c0..68f3d636 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -2,10 +2,10 @@ process GATK4_VARIANTFILTRATION { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(vcf), path(vcf_tbi) @@ -18,6 +18,9 @@ process GATK4_VARIANTFILTRATION { tuple val(meta), path("*.tbi") , emit: tbi path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/modules/gffread/main.nf b/modules/nf-core/modules/gffread/main.nf index e7893f8b..7c575c97 100644 --- a/modules/nf-core/modules/gffread/main.nf +++ b/modules/nf-core/modules/gffread/main.nf @@ -14,6 +14,9 @@ process GFFREAD { path "*.gtf" , emit: gtf path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${gff.baseName}" diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf index 77a4e546..9d4b0666 100644 --- a/modules/nf-core/modules/gunzip/main.nf +++ b/modules/nf-core/modules/gunzip/main.nf @@ -14,6 +14,9 @@ process GUNZIP { tuple val(meta), path("$gunzip"), emit: gunzip path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' gunzip = archive.toString() - '.gz' diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/modules/gunzip/meta.yml index 2e0e4054..4d2ebc84 100644 --- a/modules/nf-core/modules/gunzip/meta.yml +++ b/modules/nf-core/modules/gunzip/meta.yml @@ -5,29 +5,29 @@ keywords: - compression tools: - gunzip: - description: | - gzip is a file format and a software application used for file compression and decompression. - documentation: https://www.gnu.org/software/gzip/manual/gzip.html - licence: ["GPL-3.0-or-later"] + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ["GPL-3.0-or-later"] input: - meta: - type: map - description: | - Optional groovy Map containing meta information - e.g. [ id:'test', single_end:false ] + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] - archive: - type: file - description: File to be compressed/uncompressed - pattern: "*.*" + type: file + description: File to be compressed/uncompressed + pattern: "*.*" output: - gunzip: - type: file - description: Compressed/uncompressed file - pattern: "*.*" + type: file + description: Compressed/uncompressed file + pattern: "*.*" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf index 1264aac1..ae019dbf 100644 --- a/modules/nf-core/modules/multiqc/main.nf +++ b/modules/nf-core/modules/multiqc/main.nf @@ -28,4 +28,16 @@ process MULTIQC { multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) END_VERSIONS """ + + stub: + """ + touch multiqc_data + touch multiqc_plots + touch multiqc_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf index 7990d7e6..e754a587 100644 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ b/modules/nf-core/modules/picard/markduplicates/main.nf @@ -16,6 +16,9 @@ process PICARD_MARKDUPLICATES { tuple val(meta), path("*.metrics.txt"), emit: metrics path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" @@ -39,4 +42,17 @@ process PICARD_MARKDUPLICATES { picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + touch ${prefix}.bam.bai + touch ${prefix}.MarkDuplicates.metrics.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index 58c445d8..053279ff 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX { tag "$fasta" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(fasta) @@ -14,6 +14,9 @@ process SAMTOOLS_FAIDX { tuple val(meta), path ("*.fai"), emit: fai path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' """ @@ -26,4 +29,14 @@ process SAMTOOLS_FAIDX { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + """ + touch ${fasta}.fai + cat <<-END_VERSIONS > versions.yml + + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index 119adf77..9e3440ac 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(bam), path(bai) @@ -14,6 +14,9 @@ process SAMTOOLS_FLAGSTAT { tuple val(meta), path("*.flagstat"), emit: flagstat path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' """ diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index fc54e676..7d5cee17 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(bam), path(bai) @@ -14,6 +14,9 @@ process SAMTOOLS_IDXSTATS { tuple val(meta), path("*.idxstats"), emit: idxstats path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' """ diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index c4fa2c63..fff6e1b8 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(input) @@ -16,6 +16,9 @@ process SAMTOOLS_INDEX { tuple val(meta), path("*.crai"), optional:true, emit: crai path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' """ @@ -30,4 +33,16 @@ process SAMTOOLS_INDEX { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + """ + touch ${input}.bai + touch ${input}.crai + touch ${input}.csi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index a318bfc4..9f962a4b 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_MERGE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(input_files) @@ -16,6 +16,9 @@ process SAMTOOLS_MERGE { tuple val(meta), path("${prefix}.cram"), optional:true, emit: cram path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" @@ -35,4 +38,16 @@ process SAMTOOLS_MERGE { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}" + def file_type = input_files[0].getExtension() + """ + touch ${prefix}.${file_type} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index 42c7bbf4..ba46f0c9 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(bam) @@ -14,6 +14,9 @@ process SAMTOOLS_SORT { tuple val(meta), path("*.bam"), emit: bam path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" @@ -25,4 +28,15 @@ process SAMTOOLS_SORT { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index 7209070d..85cb64f3 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.14" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.14--hb421002_0' : - 'quay.io/biocontainers/samtools:1.14--hb421002_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : + 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" input: tuple val(meta), path(input), path(input_index) @@ -15,6 +15,9 @@ process SAMTOOLS_STATS { tuple val(meta), path("*.stats"), emit: stats path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def reference = fasta ? "--reference ${fasta}" : "" @@ -31,4 +34,15 @@ process SAMTOOLS_STATS { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${input}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/snpeff/main.nf b/modules/nf-core/modules/snpeff/main.nf index 6248fee3..1b4d5f43 100644 --- a/modules/nf-core/modules/snpeff/main.nf +++ b/modules/nf-core/modules/snpeff/main.nf @@ -17,6 +17,9 @@ process SNPEFF { path "*.csv" , emit: report path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def avail_mem = 6 diff --git a/modules/nf-core/modules/star/align/main.nf b/modules/nf-core/modules/star/align/main.nf index 9725496f..762b84f6 100644 --- a/modules/nf-core/modules/star/align/main.nf +++ b/modules/nf-core/modules/star/align/main.nf @@ -29,6 +29,10 @@ process STAR_ALIGN { tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq tuple val(meta), path('*.tab') , optional:true, emit: tab tuple val(meta), path('*.out.junction') , optional:true, emit: junction + tuple val(meta), path('*.out.sam') , optional:true, emit: sam + + when: + task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf index ad32c0dd..50d280dd 100644 --- a/modules/nf-core/modules/star/genomegenerate/main.nf +++ b/modules/nf-core/modules/star/genomegenerate/main.nf @@ -16,6 +16,9 @@ process STAR_GENOMEGENERATE { path "star" , emit: index path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def args_list = args.tokenize() diff --git a/modules/nf-core/modules/tabix/bgziptabix/main.nf b/modules/nf-core/modules/tabix/bgziptabix/main.nf index e419d153..77fd91a5 100644 --- a/modules/nf-core/modules/tabix/bgziptabix/main.nf +++ b/modules/nf-core/modules/tabix/bgziptabix/main.nf @@ -14,12 +14,15 @@ process TABIX_BGZIPTABIX { tuple val(meta), path("*.gz"), path("*.tbi"), emit: gz_tbi path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - bgzip -c $args $input > ${prefix}.gz + bgzip --threads ${task.cpus} -c $args $input > ${prefix}.gz tabix $args2 ${prefix}.gz cat <<-END_VERSIONS > versions.yml @@ -27,4 +30,16 @@ process TABIX_BGZIPTABIX { tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.gz + touch ${prefix}.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf index 6d1996e7..5aa6aa7f 100644 --- a/modules/nf-core/modules/untar/main.nf +++ b/modules/nf-core/modules/untar/main.nf @@ -2,22 +2,25 @@ process UNTAR { tag "$archive" label 'process_low' - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + conda (params.enable_conda ? "conda-forge::tar=1.32" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : 'biocontainers/biocontainers:v1.2.0_cv1' }" input: - path archive + tuple val(meta), path(archive) output: - path "$untar" , emit: untar - path "versions.yml", emit: versions + tuple val(meta), path("$untar"), emit: untar + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' - untar = archive.toString() - '.tar.gz' + untar = archive.toString() - '.tar.gz' """ tar \\ -xzvf \\ @@ -30,4 +33,15 @@ process UNTAR { untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') END_VERSIONS """ + + stub: + untar = archive.toString() - '.tar.gz' + """ + touch $untar + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/modules/untar/meta.yml index e877a97c..d426919b 100644 --- a/modules/nf-core/modules/untar/meta.yml +++ b/modules/nf-core/modules/untar/meta.yml @@ -10,11 +10,21 @@ tools: documentation: https://www.gnu.org/software/tar/manual/ licence: ["GPL-3.0-or-later"] input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - archive: type: file description: File to be untar pattern: "*.{tar}.{gz}" output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - untar: type: file description: From 4cd923e5b39f6bf6800af9c5766e42188d6f6af8 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 16:53:11 +0100 Subject: [PATCH 137/235] fix: prettier warning --- subworkflows/local/prepare_genome.nf | 15 ++++++++++----- 1 file changed, 10 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index c6c1b9a8..0f38295e 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -26,7 +26,8 @@ workflow PREPARE_GENOME { // Uncompress genome fasta file if required // if (params.fasta.endsWith('.gz')) { - ch_fasta = (GUNZIP_FASTA ( params.fasta ).gunzip) + GUNZIP_FASTA ( Channel.fromPath(params.fasta).map{ it -> [[id:it[0].baseName], it] } ) + ch_fasta = GUNZIP_FASTA.out.gunzip.map{ meta, fasta -> [fasta] } ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { ch_fasta = file(params.fasta) @@ -39,14 +40,16 @@ workflow PREPARE_GENOME { ch_gffread_version = Channel.empty() if (params.gtf) { if (params.gtf.endsWith('.gz')) { - ch_gtf = GUNZIP_GTF ( params.gtf ).gunzip + GUNZIP_GTF ( Channel.fromPath(params.gtf).map{ it -> [[id:it[0].baseName], it] } ) + ch_gtf = GUNZIP_GTF.out.gunzip.map{ meta, gtf -> [gtf] } ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { ch_gtf = file(params.gtf) } } else if (params.gff) { if (params.gff.endsWith('.gz')) { - ch_gff = GUNZIP_GFF ( params.gff ).gunzip + GUNZIP_GFF ( Channel.fromPath(params.gff).map{ it -> [[id:it[0].baseName], it] } ) + ch_gff = GUNZIP_GFF.out.gunzip.map{ meta, gff -> [gff] } ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions) } else { ch_gff = file(params.gff) @@ -60,7 +63,8 @@ workflow PREPARE_GENOME { // if (params.gene_bed) { if (params.gene_bed.endsWith('.gz')) { - ch_gene_bed = GUNZIP_GENE_BED ( params.gene_bed ).gunzip + GUNZIP_GENE_BED ( Channel.fromPath(params.gene_bed).map{ it -> [[id:it[0].baseName], it] } ) + ch_gene_bed = GUNZIP_GENE_BED.out.gunzip.map{ meta, bed -> [bed] } ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { ch_gene_bed = file(params.gene_bed) @@ -93,7 +97,8 @@ workflow PREPARE_GENOME { if ('star' in prepare_tool_indices) { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { - ch_star_index = UNTAR_STAR_INDEX ( params.star_index ).untar + UNTAR_STAR_INDEX (Channel.fromPath(params.star_index).map{ it -> [[id:it[0].baseName], it] }) + ch_star_index = UNTAR_STAR_INDEX.out.untar.map{ meta, star_index -> [star_index] }.collect() ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { ch_star_index = file(params.star_index) From 41fcc58fe92110826d2caf204ad2568c76a93a7a Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 19:04:45 +0100 Subject: [PATCH 138/235] update: local modules to DSL2 syntax; used nf-core gatk4/haplotypecaller --- modules.json | 5 +- modules/local/functions.nf | 78 ------------------- .../local/gatk4/haplotypecaller/functions.nf | 78 ------------------- modules/local/gatk4/haplotypecaller/main.nf | 62 --------------- .../local/gatk4/splitncigarreads/functions.nf | 78 ------------------- modules/local/gatk4/splitncigarreads/main.nf | 39 +++++----- modules/local/gatk4/splitncigarreads/meta.yml | 7 ++ modules/local/gtf2bed.nf | 25 +++--- modules/local/gtf_gene_filter.nf | 24 ++---- modules/local/samplesheet_check.nf | 9 ++- .../modules/gatk4/haplotypecaller/main.nf | 54 +++++++++++++ .../modules}/gatk4/haplotypecaller/meta.yml | 30 ++++--- workflows/rnavar.nf | 9 +-- 13 files changed, 132 insertions(+), 366 deletions(-) delete mode 100644 modules/local/functions.nf delete mode 100644 modules/local/gatk4/haplotypecaller/functions.nf delete mode 100644 modules/local/gatk4/haplotypecaller/main.nf delete mode 100644 modules/local/gatk4/splitncigarreads/functions.nf create mode 100644 modules/nf-core/modules/gatk4/haplotypecaller/main.nf rename modules/{local => nf-core/modules}/gatk4/haplotypecaller/meta.yml (71%) diff --git a/modules.json b/modules.json index 58e0d630..2238b854 100644 --- a/modules.json +++ b/modules.json @@ -27,6 +27,9 @@ "gatk4/createsequencedictionary": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, + "gatk4/haplotypecaller": { + "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + }, "gatk4/indexfeaturefile": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, @@ -89,4 +92,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/local/functions.nf b/modules/local/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/local/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/local/gatk4/haplotypecaller/functions.nf b/modules/local/gatk4/haplotypecaller/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/local/gatk4/haplotypecaller/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/local/gatk4/haplotypecaller/main.nf b/modules/local/gatk4/haplotypecaller/main.nf deleted file mode 100644 index fc0ee0e9..00000000 --- a/modules/local/gatk4/haplotypecaller/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - -process GATK4_HAPLOTYPECALLER { - tag "$meta.id" - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } - - input: - tuple val(meta), path(bam), path(bai), path(interval) - path dbsnp - path dbsnp_tbi - path dict - path fasta - path fai - val no_intervals - - output: - tuple val(meta), path("*.vcf.gz") , emit: vcf - tuple val(meta), path(interval), path("*.vcf.gz") , emit: interval_vcf - path "versions.yml" , emit: versions - - script: - def prefix = options.suffix ? "${interval.baseName}_${meta.id}${options.suffix}" : "${interval.baseName}_${meta.id}" - def avail_mem = 3 - if (!task.memory) { - log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - def interval_option = no_intervals ? "" : "-L ${interval}" - def dbsnp_option = dbsnp ? "-D ${dbsnp}" : "" - """ - gatk \\ - --java-options "-Xmx${avail_mem}g" \\ - HaplotypeCaller \\ - -R $fasta \\ - -I $bam \\ - ${dbsnp_option} \\ - ${interval_option} \\ - -O ${prefix}.vcf.gz \\ - $options.args \\ - --tmp-dir . - - cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/local/gatk4/splitncigarreads/functions.nf b/modules/local/gatk4/splitncigarreads/functions.nf deleted file mode 100644 index 85628ee0..00000000 --- a/modules/local/gatk4/splitncigarreads/functions.nf +++ /dev/null @@ -1,78 +0,0 @@ -// -// Utility functions used in nf-core DSL2 module files -// - -// -// Extract name of software tool from process name using $task.process -// -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -// -// Extract name of module from process name using $task.process -// -def getProcessName(task_process) { - return task_process.tokenize(':')[-1] -} - -// -// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules -// -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.args3 = args.args3 ?: '' - options.publish_by_meta = args.publish_by_meta ?: [] - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -// -// Tidy up and join elements of a list to return a path string -// -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -// -// Function to save/publish module results -// -def saveFiles(Map args) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - - // Do not publish versions.yml unless running from pytest workflow - if (args.filename.equals('versions.yml') && !System.getenv("NF_CORE_MODULES_TEST")) { - return null - } - if (ioptions.publish_by_meta) { - def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta - for (key in key_list) { - if (args.meta && key instanceof String) { - def path = key - if (args.meta.containsKey(key)) { - path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] - } - path = path instanceof String ? path : '' - path_list.add(path) - } - } - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } -} diff --git a/modules/local/gatk4/splitncigarreads/main.nf b/modules/local/gatk4/splitncigarreads/main.nf index 870b4fba..69531167 100644 --- a/modules/local/gatk4/splitncigarreads/main.nf +++ b/modules/local/gatk4/splitncigarreads/main.nf @@ -1,22 +1,11 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' - -params.options = [:] -options = initOptions(params.options) - process GATK4_SPLITNCIGARREADS { tag "$meta.id" label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } - conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" - } else { - container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" - } + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: tuple val(meta), path(bam), path(bai), path(interval) @@ -28,20 +17,30 @@ process GATK4_SPLITNCIGARREADS { tuple val(meta), path('*.bam'), emit: bam path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def interval_option = interval ? "-L ${interval}" : "" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK SplitNCigarReads] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } """ - gatk SplitNCigarReads \\ + gatk --java-options "-Xmx${avail_mem}g" SplitNCigarReads \\ -R $fasta \\ -I $bam \\ ${interval_option} \\ -O ${prefix}.bam \\ - $options.args + $args cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: - ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/local/gatk4/splitncigarreads/meta.yml b/modules/local/gatk4/splitncigarreads/meta.yml index 407e80bd..c26e9aa8 100644 --- a/modules/local/gatk4/splitncigarreads/meta.yml +++ b/modules/local/gatk4/splitncigarreads/meta.yml @@ -23,6 +23,13 @@ input: type: list description: BAM/SAM/CRAM file containing reads pattern: "*.{bam,sam,cram}" + - bai: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - interval: + type: file + description: Bed file with the genomic regions included in the library - fasta: type: file description: The reference fasta file diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index d6ceaff3..0130c97e 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -1,37 +1,30 @@ -// Import generic module functions -include { saveFiles; getProcessName } from './functions' - -params.options = [:] - process GTF2BED { tag "$gtf" label 'process_low' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/perl:5.26.2" - } else { - container "quay.io/biocontainers/perl:5.26.2" - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/perl:5.26.2' : + 'quay.io/biocontainers/perl:5.26.2' }" input: path gtf output: - path "*.bed" , emit: bed + path '*.bed' , emit: bed path "versions.yml", emit: versions - script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ + when: + task.ext.when == null || task.ext.when + + script: // This script is bundled with the pipeline, in nf-core/rnaseq/bin/ """ gtf2bed \\ $gtf \\ > ${gtf.baseName}.bed cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: + "${task.process}": perl: \$(echo \$(perl --version 2>&1) | sed 's/.*v\\(.*\\)) built.*/\\1/') END_VERSIONS """ diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf index 07138ba7..f324ace2 100644 --- a/modules/local/gtf_gene_filter.nf +++ b/modules/local/gtf_gene_filter.nf @@ -1,21 +1,10 @@ -// Import generic module functions -include { saveFiles; getProcessName } from './functions' - -params.options = [:] - process GTF_GENE_FILTER { tag "$fasta" - label "process_medium" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'genome', meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/python:3.8.3" - } else { - container "quay.io/biocontainers/python:3.8.3" - } + conda (params.enable_conda ? "conda-forge::python=3.9.5" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/python:3.9--1' : + 'quay.io/biocontainers/python:3.9--1' }" input: path fasta @@ -25,6 +14,9 @@ process GTF_GENE_FILTER { path "*.gtf" , emit: gtf path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // filter_gtf_for_genes_in_genome.py is bundled with the pipeline, in nf-core/rnaseq/bin/ """ filter_gtf_for_genes_in_genome.py \\ @@ -33,7 +25,7 @@ process GTF_GENE_FILTER { -o ${fasta.baseName}_genes.gtf cat <<-END_VERSIONS > versions.yml - ${getProcessName(task.process)}: + "${task.process}": python: \$(python --version | sed 's/Python //g') END_VERSIONS """ diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 553da8c9..76014d0d 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -1,10 +1,10 @@ process SAMPLESHEET_CHECK { tag "$samplesheet" - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + conda (params.enable_conda ? "conda-forge::python=3.9.5" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'quay.io/biocontainers/python:3.8.3' }" + 'https://depot.galaxyproject.org/singularity/python:3.9--1' : + 'quay.io/biocontainers/python:3.9--1' }" input: path samplesheet @@ -13,6 +13,9 @@ process SAMPLESHEET_CHECK { path '*.csv' , emit: csv path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/rnavar/bin/ """ check_samplesheet.py \\ diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf new file mode 100644 index 00000000..33871fcf --- /dev/null +++ b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf @@ -0,0 +1,54 @@ +process GATK4_HAPLOTYPECALLER { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + + input: + tuple val(meta), path(input), path(input_index), path(intervals) + path fasta + path fai + path dict + path dbsnp + path dbsnp_tbi + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def interval_option = intervals ? "-L ${intervals}" : "" + def dbsnp_option = dbsnp ? "-D ${dbsnp}" : "" + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk \\ + --java-options "-Xmx${avail_mem}g" \\ + HaplotypeCaller \\ + -R $fasta \\ + -I $input \\ + ${dbsnp_option} \\ + ${interval_option} \\ + -O ${prefix}.vcf.gz \\ + $args \\ + --tmp-dir . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/local/gatk4/haplotypecaller/meta.yml b/modules/nf-core/modules/gatk4/haplotypecaller/meta.yml similarity index 71% rename from modules/local/gatk4/haplotypecaller/meta.yml rename to modules/nf-core/modules/gatk4/haplotypecaller/meta.yml index 061b733e..81851a96 100644 --- a/modules/local/gatk4/haplotypecaller/meta.yml +++ b/modules/nf-core/modules/gatk4/haplotypecaller/meta.yml @@ -13,6 +13,7 @@ tools: homepage: https://gatk.broadinstitute.org/hc/en-us documentation: https://gatk.broadinstitute.org/hc/en-us/categories/360002369672s doi: 10.1158/1538-7445.AM2017-3590 + licence: ["Apache-2.0"] input: - meta: @@ -20,14 +21,17 @@ input: description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - bam: + - input: type: file - description: BAM file - pattern: "*.bam" - - bai: + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: type: file - description: Index of BAM file - pattern: "*.bam.bai" + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - intervals: + type: file + description: Bed file with the genomic regions included in the library (optional) - fasta: type: file description: The reference fasta file @@ -40,16 +44,23 @@ input: type: file description: GATK sequence dictionary pattern: "*.dict" + - dbsnp: + type: file + description: VCF file containing known sites (optional) + - dbsnp_tbi: + type: file + description: VCF index of dbsnp (optional) + output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - version: + - versions: type: file - description: File containing software version - pattern: "*.{version.txt}" + description: File containing software versions + pattern: "versions.yml" - vcf: type: file description: Compressed VCF file @@ -61,3 +72,4 @@ output: authors: - "@suzannejin" + - "@FriederikeHanssen" diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 8c4a94a4..a7df80e8 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -49,7 +49,6 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi include { INPUT_CHECK } from '../subworkflows/local/input_check' // Validate the input samplesheet.csv and prepare input channels include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' // Build the genome index and other reference files include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' // Splits reads that contain Ns in their cigar string -include { GATK4_HAPLOTYPECALLER } from '../modules/local/gatk4/haplotypecaller/main' // Haplotyper caller that runs on split interval files include { ANNOTATE } from '../subworkflows/local/annotate' // Annotate variants using snpEff or VEP or both /* @@ -64,6 +63,7 @@ include { CAT_FASTQ } from '../modules/nf-core/modules/cat/f include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' +include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/modules/gatk4/haplotypecaller/main' include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' @@ -296,12 +296,11 @@ workflow RNAVAR { GATK4_HAPLOTYPECALLER( haplotypecaller_interval_bam, - dbsnp, - dbsnp_tbi, - PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, - interval_flag + PREPARE_GENOME.out.dict, + dbsnp, + dbsnp_tbi ) haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf From 90e6290b633b42f880bf82a311c98d1c9be1f447 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 22 Mar 2022 19:06:10 +0100 Subject: [PATCH 139/235] fix: prettier warning --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index 2238b854..e2fb97f5 100644 --- a/modules.json +++ b/modules.json @@ -92,4 +92,4 @@ } } } -} \ No newline at end of file +} From 57c54254cfab31fb4b4f267b23dfd6f8820bc073 Mon Sep 17 00:00:00 2001 From: "Maxime U. Garcia" Date: Wed, 30 Mar 2022 14:13:46 +0200 Subject: [PATCH 140/235] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index a8883ed7..601cac54 100644 --- a/README.md +++ b/README.md @@ -1,4 +1,4 @@ -# ![nf-core/rnavar](docs/images/nf-core/rnavar_logo_light.png#gh-light-mode-only) ![nf-core/rnavar](docs/images/nf-core/rnavar_logo_dark.png#gh-dark-mode-only) +# ![nf-core/rnavar](docs/images/nf-core-rnavar_logo_light.png#gh-light-mode-only) ![nf-core-rnavar](docs/images/nf-core/rnavar_logo_dark.png#gh-dark-mode-only) [![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) From 8c4eeaef332fe9dc9381ddcd46ae6e152c939734 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 11:42:00 +0200 Subject: [PATCH 141/235] Removed unwanted folder --- null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt | 1 - 1 file changed, 1 deletion(-) delete mode 100644 null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt diff --git a/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt b/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt deleted file mode 100644 index 6b739acd..00000000 --- a/null/pipeline_info/execution_trace_2022-03-22_05-41-46.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar From 15dadc9a8b31df7744a60b398a9bb5b73fdb2929 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 11:42:39 +0200 Subject: [PATCH 142/235] Removed older multiqc_config.yaml --- assets/multiqc_config.yaml | 11 ----------- 1 file changed, 11 deletions(-) delete mode 100644 assets/multiqc_config.yaml diff --git a/assets/multiqc_config.yaml b/assets/multiqc_config.yaml deleted file mode 100644 index 746053ab..00000000 --- a/assets/multiqc_config.yaml +++ /dev/null @@ -1,11 +0,0 @@ -report_comment: > - This report has been generated by the nf-core/rnavar - analysis pipeline. For information about how to interpret these results, please see the - documentation. -report_section_order: - software_versions: - order: -1000 - nf-core-rnavar-summary: - order: -1001 - -export_plots: true From 1f9166811542d93ad3c8089c6aa4dc53022a5973 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 11:43:54 +0200 Subject: [PATCH 143/235] Commented out STAR version check as the logic is not working --- subworkflows/local/prepare_genome.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 0f38295e..8f0b4ad4 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -105,10 +105,10 @@ workflow PREPARE_GENOME { } } - if((!ch_star_index) || getIndexVersion(ch_star_index) != '2.7.4a'){ - ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index - ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) - } + //if((!ch_star_index) || getIndexVersion(ch_star_index) != '2.7.4a'){ + // ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index + // ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + //} } From 4e02c25a34a72a42565f6c6d8fffc21ef0d99b93 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 12:08:12 +0200 Subject: [PATCH 144/235] Updated README.md with tool versions --- README.md | 27 ++++++++++++++------------- 1 file changed, 14 insertions(+), 13 deletions(-) diff --git a/README.md b/README.md index 601cac54..558963e2 100644 --- a/README.md +++ b/README.md @@ -25,19 +25,20 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary 1. Merge re-sequenced FastQ files ([`cat`](http://www.linfo.org/cat.html)) -2. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -3. Align reads to reference genome ([`STAR`](https://github.com/alexdobin/STAR)) -4. Sort and index alignments ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) -5. Duplicate read marking ([`Picard MarkDuplicates`](https://broadinstitute.github.io/picard/)) -6. Splits reads that contain Ns in their cigar string ([`GATK4 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) -7. Estimate and correct systematic bias using base quality score recalibration ([`GATK4 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK4 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) -8. Convert a BED file to a Picard Interval List ([`Picard BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) -9. Scatter one interval-list into many interval-files ([`Picard IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) -10. Call SNPs and indels ([`GATK4 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) -11. Merge multiple VCF files into one VCF ([`GATK4 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) -12. Index the VCF ([`GATK4 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) -13. Filter variant calls based on certain criteria ([`GATK4 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) -14. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC`](http://multiqc.info/), [`R`](https://www.r-project.org/)) +2. Read QC ([`FastQC v0.11.9`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +3. Align reads to reference genome ([`STAR v2.7.9a`](https://github.com/alexdobin/STAR)) +4. Sort and index alignments ([`SAMtools v1.15`](https://sourceforge.net/projects/samtools/files/samtools/)) +5. Duplicate read marking ([`Picard MarkDuplicates v2.26.10`](https://broadinstitute.github.io/picard/)) +6. Splits reads that contain Ns in their cigar string ([`GATK v4.2.5.0 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) +7. Estimate and correct systematic bias using base quality score recalibration ([`GATK v4.2.5.0 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK v4.2.5.0 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) +8. Convert a BED file to a Picard Interval List ([`GATK v4.2.5.0 BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) +9. Scatter one interval-list into many interval-files ([`GATK v4.2.5.0 IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) +10. Call SNPs and indels ([`GATK v4.2.5.0 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) +11. Merge multiple VCF files into one VCF ([`GATK v4.2.5.0 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) +12. Index the VCF ([`GATK v4.2.5.0 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) +13. Filter variant calls based on certain criteria ([`GATK v4.2.5.0 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) +14. Annotate variants ([`snpEff v5.0`](https://pcingola.github.io/SnpEff/se_introduction/), [Ensembl VEP v104.3](https://www.ensembl.org/info/docs/tools/vep/index.html)) +15. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC v1.12`](http://multiqc.info/), [`R`](https://www.r-project.org/)) ## Quick Start From fba2597e0f8263d12d6395256a667c4cd382ee32 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 12:16:52 +0200 Subject: [PATCH 145/235] Updated gatk4/createsequencedictionary to the latest version --- modules.json | 4 ++-- .../nf-core/modules/gatk4/createsequencedictionary/main.nf | 3 +-- 2 files changed, 3 insertions(+), 4 deletions(-) diff --git a/modules.json b/modules.json index e2fb97f5..657e377f 100644 --- a/modules.json +++ b/modules.json @@ -25,7 +25,7 @@ "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/createsequencedictionary": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "fd5f6f5f4ffef4ab5a4e809bd3211bbc71c38d30" }, "gatk4/haplotypecaller": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" @@ -92,4 +92,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index fe1a6c65..dea77a1d 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -39,9 +39,8 @@ process GATK4_CREATESEQUENCEDICTIONARY { """ stub: - def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.dict + touch test.dict cat <<-END_VERSIONS > versions.yml "${task.process}": From 468ab35f4269bf5e8075628c2976c15923807225 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 12:17:49 +0200 Subject: [PATCH 146/235] Fix: prettier warning on modules.json --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index 657e377f..7d160a7a 100644 --- a/modules.json +++ b/modules.json @@ -92,4 +92,4 @@ } } } -} \ No newline at end of file +} From 19d7f24722d3e77745c7fd05925bc1d27732b122 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 16:44:17 +0200 Subject: [PATCH 147/235] Update: review feedback - Tool versions as a table --- README.md | 41 +++++++++++++++++++++++++++-------------- 1 file changed, 27 insertions(+), 14 deletions(-) diff --git a/README.md b/README.md index 558963e2..6e5b939a 100644 --- a/README.md +++ b/README.md @@ -25,20 +25,33 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Pipeline summary 1. Merge re-sequenced FastQ files ([`cat`](http://www.linfo.org/cat.html)) -2. Read QC ([`FastQC v0.11.9`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -3. Align reads to reference genome ([`STAR v2.7.9a`](https://github.com/alexdobin/STAR)) -4. Sort and index alignments ([`SAMtools v1.15`](https://sourceforge.net/projects/samtools/files/samtools/)) -5. Duplicate read marking ([`Picard MarkDuplicates v2.26.10`](https://broadinstitute.github.io/picard/)) -6. Splits reads that contain Ns in their cigar string ([`GATK v4.2.5.0 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) -7. Estimate and correct systematic bias using base quality score recalibration ([`GATK v4.2.5.0 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK v4.2.5.0 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) -8. Convert a BED file to a Picard Interval List ([`GATK v4.2.5.0 BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) -9. Scatter one interval-list into many interval-files ([`GATK v4.2.5.0 IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) -10. Call SNPs and indels ([`GATK v4.2.5.0 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) -11. Merge multiple VCF files into one VCF ([`GATK v4.2.5.0 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) -12. Index the VCF ([`GATK v4.2.5.0 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) -13. Filter variant calls based on certain criteria ([`GATK v4.2.5.0 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) -14. Annotate variants ([`snpEff v5.0`](https://pcingola.github.io/SnpEff/se_introduction/), [Ensembl VEP v104.3](https://www.ensembl.org/info/docs/tools/vep/index.html)) -15. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC v1.12`](http://multiqc.info/), [`R`](https://www.r-project.org/)) +2. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +3. Align reads to reference genome ([`STAR`](https://github.com/alexdobin/STAR)) +4. Sort and index alignments ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) +5. Duplicate read marking ([`Picard MarkDuplicates`](https://broadinstitute.github.io/picard/)) +6. Splits reads that contain Ns in their cigar string ([`GATK4 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) +7. Estimate and correct systematic bias using base quality score recalibration ([`GATK4 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK4 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) +8. Convert a BED file to a Picard Interval List ([`GATK4 BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) +9. Scatter one interval-list into many interval-files ([`GATK4 IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) +10. Call SNPs and indels ([`GATK4 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) +11. Merge multiple VCF files into one VCF ([`GATK4 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) +12. Index the VCF ([`GATK4 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) +13. Filter variant calls based on certain criteria ([`GATK4 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) +14. Annotate variants ([`snpEff`](https://pcingola.github.io/SnpEff/se_introduction/), [Ensembl VEP](https://www.ensembl.org/info/docs/tools/vep/index.html)) +15. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC`](http://multiqc.info/), [`R`](https://www.r-project.org/)) + +### Summary of tools and versions used in the pipeline: + +| Tool | Version | +|-------------|---------| +| FastQC | 0.11.9 | +| STAR | 2.7.9a | +| Samtools | 1.15 | +| Picard | 2.26.10 | +| GATK | 4.2.5.0 | +| SnpEff | 5.0 | +| Ensembl VEP | 104.3 | +| MultiQC | 1.12 | ## Quick Start From dd3fef96c589f11810b9d4014ad9ce548884a561 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 16:45:10 +0200 Subject: [PATCH 148/235] Update: review feedback - Tool versions as a table --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 6e5b939a..01c7c93d 100644 --- a/README.md +++ b/README.md @@ -40,7 +40,7 @@ On release, automated continuous integration tests run the pipeline on a full-si 14. Annotate variants ([`snpEff`](https://pcingola.github.io/SnpEff/se_introduction/), [Ensembl VEP](https://www.ensembl.org/info/docs/tools/vep/index.html)) 15. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC`](http://multiqc.info/), [`R`](https://www.r-project.org/)) -### Summary of tools and versions used in the pipeline: +### Summary of tools and version used in the pipeline: | Tool | Version | |-------------|---------| From 13c402e1b7969cedd5489093fa95743a7c215823 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 31 Mar 2022 17:01:20 +0200 Subject: [PATCH 149/235] Fix: Prettier warning on README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 01c7c93d..74c68ba5 100644 --- a/README.md +++ b/README.md @@ -43,7 +43,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ### Summary of tools and version used in the pipeline: | Tool | Version | -|-------------|---------| +| ----------- | ------- | | FastQC | 0.11.9 | | STAR | 2.7.9a | | Samtools | 1.15 | From 6fe8e209765bb9a0985e7433fe99bbcd4b31d933 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 4 Apr 2022 14:04:09 +0200 Subject: [PATCH 150/235] fix: renamed meta with interval BaseName to work correctly in MergeVCFs --- workflows/rnavar.nf | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index a7df80e8..d085536d 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -290,7 +290,8 @@ workflow RNAVAR { haplotypecaller_interval_bam = bam_variant_calling.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() - new_meta.id = meta.id + new_meta.id = meta.id + "_" + interval_list.baseName + new_meta.sample = meta.id [new_meta, bam, bai, interval_list] } @@ -305,7 +306,7 @@ workflow RNAVAR { haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf .map{ meta, vcf -> - meta.id = meta.id + meta.id = meta.sample [meta, vcf]} .groupTuple() From 96ef0ed49517ee02b50babfffc7572306e207b90 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 8 Apr 2022 10:17:23 +0200 Subject: [PATCH 151/235] Add: Else clause to call genome index if star_index is not provided --- subworkflows/local/prepare_genome.nf | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 8f0b4ad4..1cea1b5e 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -104,6 +104,10 @@ workflow PREPARE_GENOME { ch_star_index = file(params.star_index) } } + else { + ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index + ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) + } //if((!ch_star_index) || getIndexVersion(ch_star_index) != '2.7.4a'){ // ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index From 495b1717396b580ce93f7abfc74d7e4ed404f435 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 8 Apr 2022 11:41:16 +0200 Subject: [PATCH 152/235] Applied tools/pull/1510 to resolve email template error --- .nf-core.yml | 3 + .prettierignore | 9 +++ assets/email_template.html | 142 +++++++++++-------------------------- 3 files changed, 54 insertions(+), 100 deletions(-) create mode 100644 .prettierignore diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..abfd2795 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,4 @@ repository_type: pipeline +lint: + files_unchanged: + - assets/email_template.html diff --git a/.prettierignore b/.prettierignore new file mode 100644 index 00000000..d0e7ae58 --- /dev/null +++ b/.prettierignore @@ -0,0 +1,9 @@ +email_template.html +.nextflow* +work/ +data/ +results/ +.DS_Store +testing/ +testing* +*.pyc diff --git a/assets/email_template.html b/assets/email_template.html index 106a2c5f..e8bda58a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,111 +1,53 @@ - - - - + + + + - - - nf-core/rnavar Pipeline Report - - -
- + + nf-core/rnavar Pipeline Report + + +
-

nf-core/rnavar v${version}

-

Run Name: $runName

+ - <% if (!success){ out << """ -
-

nf-core/rnavar execution completed unsuccessfully!

+

nf-core/rnavar v${version}

+

Run Name: $runName

+ +<% if (!success){ + out << """ +
+

nf-core/rnavar execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

The full error message was:

- 
${errorReport}
-
- """ } else { out << """ -
+ 
${errorReport}
+
+ """ +} else { + out << """ +
nf-core/rnavar execution completed successfully! -
- """ } %> +
+ """ +} +%> -

The workflow was completed at $dateComplete (duration: $duration)

-

The command used to launch the workflow was as follows:

- 
-$commandLine
+

The workflow was completed at $dateComplete (duration: $duration)

+

The command used to launch the workflow was as follows:

+
$commandLine
-

Pipeline Configuration:

- - - <% out << summary.collect{ k,v -> " - - - - - " }.join("\n") %> - -
- $k - - 
$v
-
+

Pipeline Configuration:

+ + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
$k
$v
-

nf-core/rnavar

-

https://github.com/nf-core/rnavar

-
- +

nf-core/rnavar

+

https://github.com/nf-core/rnavar

+ +
+ + From c99182192e924eddfca90aeebf37a2e05cc6ffb3 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 8 Apr 2022 15:41:09 +0200 Subject: [PATCH 153/235] Fix: error when the input genome.fasta is a compressed .gz file --- subworkflows/local/prepare_genome.nf | 24 ++++++++++++------------ 1 file changed, 12 insertions(+), 12 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 1cea1b5e..5d42f178 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -27,10 +27,10 @@ workflow PREPARE_GENOME { // if (params.fasta.endsWith('.gz')) { GUNZIP_FASTA ( Channel.fromPath(params.fasta).map{ it -> [[id:it[0].baseName], it] } ) - ch_fasta = GUNZIP_FASTA.out.gunzip.map{ meta, fasta -> [fasta] } + ch_fasta = GUNZIP_FASTA.out.gunzip.map{ meta, fasta -> [fasta] }.collect() ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { - ch_fasta = file(params.fasta) + ch_fasta = Channel.fromPath(params.fasta).collect() } // @@ -44,15 +44,15 @@ workflow PREPARE_GENOME { ch_gtf = GUNZIP_GTF.out.gunzip.map{ meta, gtf -> [gtf] } ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { - ch_gtf = file(params.gtf) + ch_gtf = Channel.fromPath(params.gtf).collect() } } else if (params.gff) { if (params.gff.endsWith('.gz')) { GUNZIP_GFF ( Channel.fromPath(params.gff).map{ it -> [[id:it[0].baseName], it] } ) - ch_gff = GUNZIP_GFF.out.gunzip.map{ meta, gff -> [gff] } + ch_gff = GUNZIP_GFF.out.gunzip.map{ meta, gff -> [gff] }.collect() ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions) } else { - ch_gff = file(params.gff) + ch_gff = Channel.fromPath(params.gff).collect() } ch_gtf = GFFREAD ( ch_gff ).gtf ch_versions = ch_versions.mix(GFFREAD.out.versions) @@ -64,28 +64,28 @@ workflow PREPARE_GENOME { if (params.gene_bed) { if (params.gene_bed.endsWith('.gz')) { GUNZIP_GENE_BED ( Channel.fromPath(params.gene_bed).map{ it -> [[id:it[0].baseName], it] } ) - ch_gene_bed = GUNZIP_GENE_BED.out.gunzip.map{ meta, bed -> [bed] } + ch_gene_bed = GUNZIP_GENE_BED.out.gunzip.map{ meta, bed -> [bed] }.collect() ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { - ch_gene_bed = file(params.gene_bed) + ch_gene_bed = Channel.fromPath(params.gene_bed).collect() } } else { - ch_gene_bed = GTF2BED ( ch_gtf ).bed + ch_gene_bed = GTF2BED ( ch_gtf ).bed.collect() ch_versions = ch_versions.mix(GTF2BED.out.versions) } // Index the genome fasta ch_fasta_fai = Channel.empty() - if (params.fasta_fai) ch_fasta_fai = file(params.fasta_fai) + if (params.fasta_fai) ch_fasta_fai = Channel.fromPath(params.fasta_fai).collect() if (!params.fasta_fai) { - SAMTOOLS_FAIDX(Channel.fromPath(ch_fasta).map{ it -> [[id:it[0].getName()], it]}) + SAMTOOLS_FAIDX(ch_fasta.map{ it -> [[id:it[0].getName()], it]}) ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] }.collect() ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) } // Create dictionary file for the genome fasta ch_fasta_dict = Channel.empty() - if (params.dict) ch_fasta_dict = file(params.dict) + if (params.dict) ch_fasta_dict = Channel.fromPath(params.dict).collect() else ch_fasta_dict = GATK4_CREATESEQUENCEDICTIONARY(ch_fasta).dict // @@ -101,7 +101,7 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX.out.untar.map{ meta, star_index -> [star_index] }.collect() ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = file(params.star_index) + ch_star_index = Channel.fromPath(params.star_index).collect() } } else { From 05221376c115a52324d7c0bd1bf3f68bc0646462 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 8 Apr 2022 15:58:27 +0200 Subject: [PATCH 154/235] Add: collect() to gtf --- subworkflows/local/prepare_genome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 5d42f178..394ca2e9 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -41,7 +41,7 @@ workflow PREPARE_GENOME { if (params.gtf) { if (params.gtf.endsWith('.gz')) { GUNZIP_GTF ( Channel.fromPath(params.gtf).map{ it -> [[id:it[0].baseName], it] } ) - ch_gtf = GUNZIP_GTF.out.gunzip.map{ meta, gtf -> [gtf] } + ch_gtf = GUNZIP_GTF.out.gunzip.map{ meta, gtf -> [gtf] }.collect() ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { ch_gtf = Channel.fromPath(params.gtf).collect() From 8beea4841ef9e531c19daff1f992febc071d7476 Mon Sep 17 00:00:00 2001 From: Praveen Date: Sat, 9 Apr 2022 00:02:31 +0200 Subject: [PATCH 155/235] Add: Limit parameters for STAR to manage memory failures; BAM in Coord sorted --- conf/modules.config | 7 +++++-- docs/usage.md | 2 ++ nextflow.config | 3 +++ nextflow_schema.json | 17 +++++++++++++++++ 4 files changed, 27 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f04554df..1c094924 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -141,11 +141,14 @@ process { withName: STAR_ALIGN { ext.args = [ - '--outSAMtype BAM Unsorted', + '--outSAMtype BAM SortedByCoordinate', '--readFilesCommand zcat', params.save_unaligned ? '--outReadsUnmapped Fastx' : '', params.read_length ? "--sjdbOverhang ${params.read_length - 1}" : '', - params.star_twopass ? '--twopassMode Basic' : '' + params.star_twopass ? '--twopassMode Basic' : '', + params.star_limitBAMsortRAM > 0 ? "--limitBAMsortRAM ${params.star_limitBAMsortRAM}" : "", + params.star_outBAMsortingBinsN > 0 ? "--outBAMsortingBinsN ${params.star_outBAMsortingBinsN}" : "", + params.star_limitOutSJcollapsed > 0 ? "--limitOutSJcollapsed ${params.star_limitOutSJcollapsed}" : "" ].join(' ').trim() publishDir = [ [ diff --git a/docs/usage.md b/docs/usage.md index d35bb8ef..d9e66ac5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -116,6 +116,8 @@ Read length is an important parameter therefore it has to be used carefully. The > **NB:** Read length `--read_length` is an important parameter, therefore it has to set according to the input read length. If you are supplying a pre-built genome index, please make sure that you have used the same (read_length -1) during the genomeGenerate step. +STAR alignment generates a coordinated-sorted BAM file as output. The coordinate-sorting process can be very memory intensive when the input data is deep sequenced or the genome has many highly expressed loci. When the pipeline runs on memory constrained environment, sorting step may fail due to low memory. In such cases you may adjust the limit parameters such as `--star_limitBAMsortRAM`, `--star_outBAMsortingBinsN` and `--star_limitOutSJcollapsed` to increase the sorting memory and genomic bins. Refer the parameter documentation for the default values and adjust as appropriate based on your memory availability. + ## Preprocessing options Marking duplicate reads is performed using `Picard MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. diff --git a/nextflow.config b/nextflow.config index 52cf896e..d7cbe944 100644 --- a/nextflow.config +++ b/nextflow.config @@ -28,6 +28,9 @@ params { aligner = 'star' // Only STAR is currently supported. star_twopass = true star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR + star_limitBAMsortRAM = 0 + star_outBAMsortingBinsN = 50 + star_limitOutSJcollapsed = 1000000 seq_center = null seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work bam_csi_index = false diff --git a/nextflow_schema.json b/nextflow_schema.json index a98535d7..5acf4ef1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -186,6 +186,23 @@ "description": "Do not use GTF file during STAR index buidling step", "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." }, + "star_limitBAMsortRAM": { + "type": "integer", + "default": 0, + "description": "Option to limit RAM when sorting BAM file. Value to be specified in bytes. If 0, will be set to the genome index size.", + "help_text": "This parameter specifies the maximum available RAM (bytes) for sorting BAM during STAR alignment." + }, + "star_outBAMsortingBinsN": { + "type": "integer", + "default": 50, + "description": "Specifies the number of genome bins for coordinate-sorting", + "help_text": "This parameter specifies the number of bins to be used for coordinate sorting during STAR alignment step." + }, + "star_limitOutSJcollapsed": { + "type": "integer", + "default": 1000000, + "description": "Specifies the maximum number of collapsed junctions" + }, "seq_center": { "type": "string", "description": "Sequencing center information to be added to read group of BAM files.", From 75de1943fabaa24f1030f3ec641a4f9226112674 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 11:05:09 +0200 Subject: [PATCH 156/235] Use vep_genome and snpeff_db when --genome is not specified --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 1c094924..ba366246 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -340,13 +340,13 @@ process { withName: 'ENSEMBLVEP' { ext.args = '--everything --filter_common --per_gene --total_length --offline' - container = { "nfcore/vep:104.3.${params.genome}" } + container = { params.genome ? "nfcore/vep:104.3.${params.genome}" : "nfcore/vep:104.3.${params.vep_genome}" } publishDir = [ enabled: false ] } withName: 'SNPEFF' { ext.args = '-nodownload -canon -v' - container = { "nfcore/snpeff:5.0.${params.genome}" } + container = { params.genome ? "nfcore/snpeff:5.0.${params.genome}" : "nfcore/snpeff:5.0.${params.snpeff_db.split('\\.')[0]}" } publishDir = [ enabled: false ] } From eb7f004b7a4819c53a7e7ec7424ff035124760e6 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 11:10:29 +0200 Subject: [PATCH 157/235] Fix: samplesheet_test.csv link --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index d9e66ac5..9704ae81 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -82,7 +82,7 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,reverse | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `strandedness` | Sample strand-specificity. Must be one of `unstranded`, `forward` or `reverse`. | -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. +An [example samplesheet](../assets/samplesheet_test.csv) has been provided with the pipeline. > **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. From 8f58654fa68e7b8c1b1ebe7ba89f91e4f9fd1c38 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 11:29:03 +0200 Subject: [PATCH 158/235] Update: nf-core modules - #1 --- modules.json | 20 +++++++++---------- .../nf-core/modules/samtools/faidx/main.nf | 6 +++--- .../nf-core/modules/samtools/flagstat/main.nf | 6 +++--- .../nf-core/modules/samtools/idxstats/main.nf | 6 +++--- .../nf-core/modules/samtools/index/main.nf | 6 +++--- .../nf-core/modules/samtools/merge/main.nf | 6 +++--- modules/nf-core/modules/samtools/sort/main.nf | 6 +++--- .../nf-core/modules/samtools/stats/main.nf | 6 +++--- .../modules/star/genomegenerate/main.nf | 6 +++--- modules/nf-core/modules/untar/main.nf | 6 +++--- 10 files changed, 37 insertions(+), 37 deletions(-) diff --git a/modules.json b/modules.json index 7d160a7a..3cdd2016 100644 --- a/modules.json +++ b/modules.json @@ -55,25 +55,25 @@ "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "samtools/faidx": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/flagstat": { - "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/idxstats": { - "git_sha": "1ad73f1b2abdea9398680d6d20014838135c9a35" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/index": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/merge": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/sort": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "samtools/stats": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "snpeff": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" @@ -82,14 +82,14 @@ "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614" }, "star/genomegenerate": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "tabix/bgziptabix": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "untar": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "9ae34a01d1747019fd37753ff4cafb05aec35a2b" } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/samtools/faidx/main.nf b/modules/nf-core/modules/samtools/faidx/main.nf index 053279ff..fdce7d9b 100644 --- a/modules/nf-core/modules/samtools/faidx/main.nf +++ b/modules/nf-core/modules/samtools/faidx/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FAIDX { tag "$fasta" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/modules/samtools/flagstat/main.nf index 9e3440ac..b87b2108 100644 --- a/modules/nf-core/modules/samtools/flagstat/main.nf +++ b/modules/nf-core/modules/samtools/flagstat/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/modules/samtools/idxstats/main.nf index 7d5cee17..a49ff35f 100644 --- a/modules/nf-core/modules/samtools/idxstats/main.nf +++ b/modules/nf-core/modules/samtools/idxstats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/modules/samtools/index/main.nf index fff6e1b8..e04e63e8 100644 --- a/modules/nf-core/modules/samtools/index/main.nf +++ b/modules/nf-core/modules/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/modules/samtools/merge/main.nf b/modules/nf-core/modules/samtools/merge/main.nf index 9f962a4b..bbf7e8fb 100644 --- a/modules/nf-core/modules/samtools/merge/main.nf +++ b/modules/nf-core/modules/samtools/merge/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_MERGE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(input_files) diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/modules/samtools/sort/main.nf index ba46f0c9..b4fc1cbe 100644 --- a/modules/nf-core/modules/samtools/sort/main.nf +++ b/modules/nf-core/modules/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/modules/samtools/stats/main.nf index 85cb64f3..bbdc3240 100644 --- a/modules/nf-core/modules/samtools/stats/main.nf +++ b/modules/nf-core/modules/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15" : null) + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' : - 'quay.io/biocontainers/samtools:1.15--h1170115_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/modules/star/genomegenerate/main.nf b/modules/nf-core/modules/star/genomegenerate/main.nf index 50d280dd..e5568f1d 100644 --- a/modules/nf-core/modules/star/genomegenerate/main.nf +++ b/modules/nf-core/modules/star/genomegenerate/main.nf @@ -3,10 +3,10 @@ process STAR_GENOMEGENERATE { label 'process_high' // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.13 conda-forge::gawk=5.1.0" : null) + conda (params.enable_conda ? "bioconda::star=2.7.9a bioconda::samtools=1.15.1 conda-forge::gawk=5.1.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' : - 'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:a7908dfb0485a80ca94e4d17b0ac991532e4e989-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:1c4c32d87798d425c970ececfbadd155e7560277-0' : + 'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:1c4c32d87798d425c970ececfbadd155e7560277-0' }" input: path fasta diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf index 5aa6aa7f..bbfa0bfe 100644 --- a/modules/nf-core/modules/untar/main.nf +++ b/modules/nf-core/modules/untar/main.nf @@ -2,10 +2,10 @@ process UNTAR { tag "$archive" label 'process_low' - conda (params.enable_conda ? "conda-forge::tar=1.32" : null) + conda (params.enable_conda ? "conda-forge::tar=1.34" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : - 'biocontainers/biocontainers:v1.2.0_cv1' }" + 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv2/biocontainers_v1.2.0_cv2.img' : + 'biocontainers/biocontainers:v1.2.0_cv2' }" input: tuple val(meta), path(archive) From 61274f37cba89afababb22116cbbbd754a1a419e Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 12:01:42 +0200 Subject: [PATCH 159/235] Update: nf-core modules - #2 --- modules.json | 10 +++++----- .../modules/gatk4/createsequencedictionary/main.nf | 9 +++++---- modules/nf-core/modules/gatk4/indexfeaturefile/main.nf | 9 +++++---- .../nf-core/modules/gatk4/variantfiltration/main.nf | 10 ++++++---- 4 files changed, 21 insertions(+), 17 deletions(-) diff --git a/modules.json b/modules.json index 3cdd2016..3c59c4e9 100644 --- a/modules.json +++ b/modules.json @@ -19,19 +19,19 @@ "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/baserecalibrator": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/bedtointervallist": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/createsequencedictionary": { - "git_sha": "fd5f6f5f4ffef4ab5a4e809bd3211bbc71c38d30" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/haplotypecaller": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/indexfeaturefile": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/intervallisttools": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" @@ -40,7 +40,7 @@ "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gatk4/variantfiltration": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gffread": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index dea77a1d..dbf37048 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -11,14 +11,15 @@ process GATK4_CREATESEQUENCEDICTIONARY { path fasta output: - path "*.dict" , emit: dict - path "versions.yml" , emit: versions + path "*.dict" , emit: dict + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' + def avail_mem = 6 if (!task.memory) { log.info '[GATK CreateSequenceDictionary] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.' @@ -26,10 +27,10 @@ process GATK4_CREATESEQUENCEDICTIONARY { avail_mem = task.memory.giga } """ - gatk --java-options "-Xmx${avail_mem}g" \\ - CreateSequenceDictionary \\ + gatk --java-options "-Xmx${avail_mem}g" CreateSequenceDictionary \\ --REFERENCE $fasta \\ --URI $fasta \\ + --TMP_DIR . \\ $args cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index 275e51f5..90ff94e6 100644 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -19,6 +19,7 @@ process GATK4_INDEXFEATUREFILE { script: def args = task.ext.args ?: '' + def avail_mem = 3 if (!task.memory) { log.info '[GATK IndexFeatureFile] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -26,10 +27,10 @@ process GATK4_INDEXFEATUREFILE { avail_mem = task.memory.giga } """ - gatk --java-options "-Xmx${avail_mem}g" \\ - IndexFeatureFile \\ - $args \\ - -I $feature_file + gatk --java-options "-Xmx${avail_mem}g" IndexFeatureFile \\ + --input $feature_file \\ + --tmp-dir . \\ + $args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index 68f3d636..6beb87ef 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -8,7 +8,7 @@ process GATK4_VARIANTFILTRATION { 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: - tuple val(meta), path(vcf), path(vcf_tbi) + tuple val(meta), path(vcf), path(tbi) path fasta path fai path dict @@ -24,6 +24,7 @@ process GATK4_VARIANTFILTRATION { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def avail_mem = 3 if (!task.memory) { log.info '[GATK VariantFiltration] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -32,9 +33,10 @@ process GATK4_VARIANTFILTRATION { } """ gatk --java-options "-Xmx${avail_mem}G" VariantFiltration \\ - -R $fasta \\ - -V $vcf \\ - -O ${prefix}.vcf.gz \\ + --variant $vcf \\ + --output ${prefix}.vcf.gz \\ + --reference $fasta \\ + --tmp-dir . \\ $args cat <<-END_VERSIONS > versions.yml From 89a83f64e35f3afeab23a28fa3864fa6c35a3b76 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 12:44:22 +0200 Subject: [PATCH 160/235] Update: nf-core modules - #3 --- modules.json | 8 ++--- .../nf-core/modules/gatk4/applybqsr/main.nf | 15 ++++---- .../nf-core/modules/gatk4/applybqsr/meta.yml | 4 +++ .../modules/gatk4/baserecalibrator/main.nf | 33 +++++++++-------- .../modules/gatk4/baserecalibrator/meta.yml | 10 ++++-- .../modules/gatk4/bedtointervallist/main.nf | 10 +++--- .../modules/gatk4/haplotypecaller/main.nf | 35 +++++++++---------- .../modules/gatk4/intervallisttools/main.nf | 13 +++---- .../nf-core/modules/gatk4/mergevcfs/main.nf | 20 +++++------ workflows/rnavar.nf | 4 +-- 10 files changed, 78 insertions(+), 74 deletions(-) diff --git a/modules.json b/modules.json index 3c59c4e9..db3c3d1c 100644 --- a/modules.json +++ b/modules.json @@ -16,13 +16,13 @@ "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/applybqsr": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/baserecalibrator": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/bedtointervallist": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/createsequencedictionary": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" @@ -34,10 +34,10 @@ "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/intervallisttools": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/mergevcfs": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/variantfiltration": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index 851afc04..7a64dab2 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -14,9 +14,9 @@ process GATK4_APPLYBQSR { path dict output: - tuple val(meta), path("*.bam"), emit: bam, optional: true + tuple val(meta), path("*.bam") , emit: bam, optional: true tuple val(meta), path("*.cram"), emit: cram, optional: true - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -24,8 +24,7 @@ process GATK4_APPLYBQSR { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def interval = intervals ? "-L ${intervals}" : "" - def file_type = input.getExtension() + def interval_command = intervals ? "--intervals $intervals" : "" def avail_mem = 3 if (!task.memory) { @@ -35,12 +34,12 @@ process GATK4_APPLYBQSR { } """ gatk --java-options "-Xmx${avail_mem}g" ApplyBQSR \\ - -R $fasta \\ - -I $input \\ + --input $input \\ + --output ${prefix}.${input.getExtension()} \\ + --reference $fasta \\ --bqsr-recal-file $bqsr_table \\ - $interval \\ + $interval_command \\ --tmp-dir . \\ - -O ${prefix}.${file_type} \\ $args cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/modules/gatk4/applybqsr/meta.yml b/modules/nf-core/modules/gatk4/applybqsr/meta.yml index 82d3cbf3..3fc93f10 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/meta.yml +++ b/modules/nf-core/modules/gatk4/applybqsr/meta.yml @@ -61,6 +61,10 @@ output: type: file description: Recalibrated BAM file pattern: "*.{bam}" + - cram: + type: file + description: Recalibrated CRAM file + pattern: "*.{cram}" authors: - "@yocra3" diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index ecb41d9b..766a8338 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -9,15 +9,15 @@ process GATK4_BASERECALIBRATOR { input: tuple val(meta), path(input), path(input_index), path(intervals) - path fasta - path fai - path dict - path knownSites - path knownSites_tbi + path fasta + path fai + path dict + path known_sites + path known_sites_tbi output: tuple val(meta), path("*.table"), emit: table - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -25,8 +25,8 @@ process GATK4_BASERECALIBRATOR { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def intervalsCommand = intervals ? "-L ${intervals}" : "" - def sitesCommand = knownSites.collect{"--known-sites ${it}"}.join(' ') + def interval_command = intervals ? "--intervals $intervals" : "" + def sites_command = known_sites.collect{"--known-sites $it"}.join(' ') def avail_mem = 3 if (!task.memory) { @@ -34,16 +34,15 @@ process GATK4_BASERECALIBRATOR { } else { avail_mem = task.memory.giga } - """ - gatk --java-options "-Xmx${avail_mem}g" BaseRecalibrator \ - -R $fasta \ - -I $input \ - $sitesCommand \ - $intervalsCommand \ - --tmp-dir . \ - $args \ - -O ${prefix}.table + gatk --java-options "-Xmx${avail_mem}g" BaseRecalibrator \\ + --input $input \\ + --output ${prefix}.table \\ + --reference $fasta \\ + $interval_command \\ + $sites_command \\ + --tmp-dir . \\ + $args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml index 2e52b8ab..08c1ebbf 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml +++ b/modules/nf-core/modules/gatk4/baserecalibrator/meta.yml @@ -42,9 +42,14 @@ input: type: file description: GATK sequence dictionary pattern: "*.dict" - - knownSites: + - known_sites: type: file - description: Bed file with the genomic regions included in the library (optional) + description: VCF files with known sites for indels / snps (optional) + pattern: "*.vcf.gz" + - known_sites_tbi: + type: file + description: Tabix index of the known_sites (optional) + pattern: "*.vcf.gz.tbi" output: - meta: @@ -64,3 +69,4 @@ output: authors: - "@yocra3" - "@FriederikeHanssen" + - "@maxulysse" diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index c3b624a8..118f535b 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -9,7 +9,7 @@ process GATK4_BEDTOINTERVALLIST { input: tuple val(meta), path(bed) - path sequence_dict + path dict output: tuple val(meta), path('*.interval_list'), emit: interval_list @@ -21,6 +21,7 @@ process GATK4_BEDTOINTERVALLIST { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def avail_mem = 3 if (!task.memory) { log.info '[GATK BedToIntervalList] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -29,9 +30,10 @@ process GATK4_BEDTOINTERVALLIST { } """ gatk --java-options "-Xmx${avail_mem}g" BedToIntervalList \\ - -I $bed \\ - -SD $sequence_dict \\ - -O ${prefix}.interval_list \\ + --INPUT $bed \\ + --OUTPUT ${prefix}.interval_list \\ + --SEQUENCE_DICTIONARY $dict \\ + --TMP_DIR . \\ $args cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf index 33871fcf..57f69ecd 100644 --- a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf +++ b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf @@ -9,11 +9,11 @@ process GATK4_HAPLOTYPECALLER { input: tuple val(meta), path(input), path(input_index), path(intervals) - path fasta - path fai - path dict - path dbsnp - path dbsnp_tbi + path fasta + path fai + path dict + path dbsnp + path dbsnp_tbi output: tuple val(meta), path("*.vcf.gz"), emit: vcf @@ -26,25 +26,24 @@ process GATK4_HAPLOTYPECALLER { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def interval_option = intervals ? "-L ${intervals}" : "" - def dbsnp_option = dbsnp ? "-D ${dbsnp}" : "" - def avail_mem = 3 + def dbsnp_command = dbsnp ? "--dbsnp $dbsnp" : "" + def interval_command = intervals ? "--intervals $intervals" : "" + + def avail_mem = 3 if (!task.memory) { log.info '[GATK HaplotypeCaller] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ - gatk \\ - --java-options "-Xmx${avail_mem}g" \\ - HaplotypeCaller \\ - -R $fasta \\ - -I $input \\ - ${dbsnp_option} \\ - ${interval_option} \\ - -O ${prefix}.vcf.gz \\ - $args \\ - --tmp-dir . + gatk --java-options "-Xmx${avail_mem}g" HaplotypeCaller \\ + --input $input \\ + --output ${prefix}.vcf.gz \\ + --reference $fasta \\ + $dbsnp_command \\ + $interval_command \\ + --tmp-dir . \\ + $args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index 82c3222c..1b9b37f4 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -8,11 +8,11 @@ process GATK4_INTERVALLISTTOOLS { 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: - tuple val(meta), path(interval_list) + tuple val(meta), path(intervals) output: tuple val(meta), path("*_split/*/*.interval_list"), emit: interval_list - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -20,6 +20,7 @@ process GATK4_INTERVALLISTTOOLS { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def avail_mem = 3 if (!task.memory) { log.info '[GATK IntervalListTools] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -30,10 +31,10 @@ process GATK4_INTERVALLISTTOOLS { mkdir ${prefix}_split - gatk --java-options "-Xmx${avail_mem}g" \\ - IntervalListTools \\ - -I ${interval_list} \\ - -O ${prefix}_split \\ + gatk --java-options "-Xmx${avail_mem}g" IntervalListTools \\ + --INPUT $intervals \\ + --OUTPUT ${prefix}_split \\ + --TMP_DIR . \\ $args python3 < versions.yml diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index d085536d..fc92918c 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -311,12 +311,10 @@ workflow RNAVAR { .groupTuple() ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLER.out.versions.first().ifEmpty(null)) - use_ref_dict = true GATK4_MERGEVCFS( haplotypecaller_raw, - PREPARE_GENOME.out.dict, - use_ref_dict + PREPARE_GENOME.out.dict ) haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) From 32f175f8d6d5c2ca9773d12bb994db6600bfc282 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 15:30:36 +0200 Subject: [PATCH 161/235] Changed logic for creating GTF2BED at exon level --- bin/gtf2bed | 122 --------------------------- conf/test.config | 1 - docs/usage.md | 2 +- main.nf | 2 +- modules/local/gtf2bed.nf | 19 +++-- nextflow_schema.json | 4 +- subworkflows/local/prepare_genome.nf | 14 +-- workflows/rnavar.nf | 2 +- 8 files changed, 24 insertions(+), 142 deletions(-) delete mode 100755 bin/gtf2bed diff --git a/bin/gtf2bed b/bin/gtf2bed deleted file mode 100755 index 4da5c046..00000000 --- a/bin/gtf2bed +++ /dev/null @@ -1,122 +0,0 @@ -#!/usr/bin/env perl - -# Copyright (c) 2011 Erik Aronesty (erik@q32.com) -# -# Permission is hereby granted, free of charge, to any person obtaining a copy -# of this software and associated documentation files (the "Software"), to deal -# in the Software without restriction, including without limitation the rights -# to use, copy, modify, merge, publish, distribute, sublicense, and/or sell -# copies of the Software, and to permit persons to whom the Software is -# furnished to do so, subject to the following conditions: -# -# The above copyright notice and this permission notice shall be included in -# all copies or substantial portions of the Software. -# -# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR -# IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, -# FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE -# AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER -# LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, -# OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN -# THE SOFTWARE. -# -# ALSO, IT WOULD BE NICE IF YOU LET ME KNOW YOU USED IT. - -use Getopt::Long; - -my $extended; -GetOptions("x"=>\$extended); - -$in = shift @ARGV; - -my $in_cmd =($in =~ /\.gz$/ ? "gunzip -c $in|" : $in =~ /\.zip$/ ? "unzip -p $in|" : "$in") || die "Can't open $in: $!\n"; -open IN, $in_cmd; - -while () { - $gff = 2 if /^##gff-version 2/; - $gff = 3 if /^##gff-version 3/; - next if /^#/ && $gff; - - s/\s+$//; - # 0-chr 1-src 2-feat 3-beg 4-end 5-scor 6-dir 7-fram 8-attr - my @f = split /\t/; - if ($gff) { - # most ver 2's stick gene names in the id field - ($id) = $f[8]=~ /\bID="([^"]+)"/; - # most ver 3's stick unquoted names in the name field - ($id) = $f[8]=~ /\bName=([^";]+)/ if !$id && $gff == 3; - } else { - ($id) = $f[8]=~ /transcript_id "([^"]+)"/; - } - - next unless $id && $f[0]; - - if ($f[2] eq 'exon') { - die "no position at exon on line $." if ! $f[3]; - # gff3 puts :\d in exons sometimes - $id =~ s/:\d+$// if $gff == 3; - push @{$exons{$id}}, \@f; - # save lowest start - $trans{$id} = \@f if !$trans{$id}; - } elsif ($f[2] eq 'start_codon') { - #optional, output codon start/stop as "thick" region in bed - $sc{$id}->[0] = $f[3]; - } elsif ($f[2] eq 'stop_codon') { - $sc{$id}->[1] = $f[4]; - } elsif ($f[2] eq 'miRNA' ) { - $trans{$id} = \@f if !$trans{$id}; - push @{$exons{$id}}, \@f; - } -} - -for $id ( - # sort by chr then pos - sort { - $trans{$a}->[0] eq $trans{$b}->[0] ? - $trans{$a}->[3] <=> $trans{$b}->[3] : - $trans{$a}->[0] cmp $trans{$b}->[0] - } (keys(%trans)) ) { - my ($chr, undef, undef, undef, undef, undef, $dir, undef, $attr, undef, $cds, $cde) = @{$trans{$id}}; - my ($cds, $cde); - ($cds, $cde) = @{$sc{$id}} if $sc{$id}; - - # sort by pos - my @ex = sort { - $a->[3] <=> $b->[3] - } @{$exons{$id}}; - - my $beg = $ex[0][3]; - my $end = $ex[-1][4]; - - if ($dir eq '-') { - # swap - $tmp=$cds; - $cds=$cde; - $cde=$tmp; - $cds -= 2 if $cds; - $cde += 2 if $cde; - } - - # not specified, just use exons - $cds = $beg if !$cds; - $cde = $end if !$cde; - - # adjust start for bed - --$beg; --$cds; - - my $exn = @ex; # exon count - my $exst = join ",", map {$_->[3]-$beg-1} @ex; # exon start - my $exsz = join ",", map {$_->[4]-$_->[3]+1} @ex; # exon size - - my $gene_id; - my $extend = ""; - if ($extended) { - ($gene_id) = $attr =~ /gene_name "([^"]+)"/; - ($gene_id) = $attr =~ /gene_id "([^"]+)"/ unless $gene_id; - $extend="\t$gene_id"; - } - # added an extra comma to make it look exactly like ucsc's beds - print "$chr\t$beg\t$end\t$id\t0\t$dir\t$cds\t$cde\t0\t$exn\t$exsz,\t$exst,$extend\n"; -} - -close IN; diff --git a/conf/test.config b/conf/test.config index 0c881dc7..59c63832 100644 --- a/conf/test.config +++ b/conf/test.config @@ -28,7 +28,6 @@ params { //fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' - gene_bed = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.bed' // Known genome resources (optional) dbsnp = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz' diff --git a/docs/usage.md b/docs/usage.md index 9704ae81..4a7c6dc1 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -100,7 +100,7 @@ The index building step can be quite a time-consuming process and it permits the - If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. - If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. -- If `--gene_bed` is not provided then it will be generated from the GTF file. +- If `--exon_bed` is not provided then it will be generated from the GTF file. - If `--star_index` is not provided then it will be generated from the reference genome FASTA file using `STAR --runmode genomeGenerate` command. > **NB:** In case if you are providing a GTF and/or a BED file, please ensure that the chromosomes and contigs in the files are also present in the genome FASTA (and in the .dict) file. Otherwise `GATK BedToIntervalList` module is likely to fail if the chromosomes/contigs do not match with the reference genome data. diff --git a/main.nf b/main.nf index d26340f1..65ee5089 100644 --- a/main.nf +++ b/main.nf @@ -21,7 +21,7 @@ params.fasta_fai = WorkflowMain.getGenomeAttribute(params, 'fasta_fai params.dict = WorkflowMain.getGenomeAttribute(params, 'dict') params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') -params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'bed12') +params.exon_bed = WorkflowMain.getGenomeAttribute(params, 'exon_bed') params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') params.dbsnp = WorkflowMain.getGenomeAttribute(params, 'dbsnp') params.dbsnp_tbi = WorkflowMain.getGenomeAttribute(params, 'dbsnp_tbi') diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index 0130c97e..0c434dcb 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -2,10 +2,10 @@ process GTF2BED { tag "$gtf" label 'process_low' - conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) + conda (params.enable_conda ? "conda-forge::r-base=3.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/perl:5.26.2' : - 'quay.io/biocontainers/perl:5.26.2' }" + 'https://depot.galaxyproject.org/singularity/r-base:3.5.0' : + 'quay.io/biocontainers/r-base:3.5.0' }" input: path gtf @@ -19,13 +19,18 @@ process GTF2BED { script: // This script is bundled with the pipeline, in nf-core/rnaseq/bin/ """ - gtf2bed \\ - $gtf \\ - > ${gtf.baseName}.bed + Rscript --no-save -<<'RCODE' + gtf = read.table("${gtf}", sep="\t") + gtf = subset(gtf, V3 == "exon") + write.table(data.frame(chrom=gtf[,'V1'], start=gtf[,'V4'], end=gtf[,'V5']), "tmp.exome.bed", quote = F, sep="\t", col.names = F, row.names = F) + RCODE + + awk '{print \$1 "\t" (\$2 - 1) "\t" \$3}' tmp.exome.bed > exome.bed + rm tmp.exome.bed cat <<-END_VERSIONS > versions.yml "${task.process}": - perl: \$(echo \$(perl --version 2>&1) | sed 's/.*v\\(.*\\)) built.*/\\1/') + Rscript: \$(echo \$(Rscript --version 2>&1) | sed 's/R scripting front-end version //') END_VERSIONS """ } diff --git a/nextflow_schema.json b/nextflow_schema.json index 5acf4ef1..fa2466b9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -104,9 +104,9 @@ "description": "Path to GFF3 annotation file.", "help_text": "This parameter must be specified if `--genome` or `--gtf` are not specified." }, - "gene_bed": { + "exon_bed": { "type": "string", - "description": "Path to BED file containing gene intervals. This will be created from the GTF file if not specified." + "description": "Path to BED file containing exon intervals. This will be created from the GTF file if not specified." }, "read_length": { "type": "number", diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 394ca2e9..b0721aaf 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -59,18 +59,18 @@ workflow PREPARE_GENOME { } // - // Uncompress gene BED annotation file or create from GTF if required + // Uncompress exon BED annotation file or create from GTF if required // - if (params.gene_bed) { - if (params.gene_bed.endsWith('.gz')) { - GUNZIP_GENE_BED ( Channel.fromPath(params.gene_bed).map{ it -> [[id:it[0].baseName], it] } ) + if (params.exon_bed) { + if (params.exon_bed.endsWith('.gz')) { + GUNZIP_GENE_BED ( Channel.fromPath(params.exon_bed).map{ it -> [[id:it[0].baseName], it] } ) ch_gene_bed = GUNZIP_GENE_BED.out.gunzip.map{ meta, bed -> [bed] }.collect() ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { - ch_gene_bed = Channel.fromPath(params.gene_bed).collect() + ch_gene_bed = Channel.fromPath(params.exon_bed).collect() } } else { - ch_gene_bed = GTF2BED ( ch_gtf ).bed.collect() + ch_exon_bed = GTF2BED ( ch_gtf ).bed.collect() ch_versions = ch_versions.mix(GTF2BED.out.versions) } @@ -121,7 +121,7 @@ workflow PREPARE_GENOME { fai = ch_fasta_fai // path: genome.fasta.fai dict = ch_fasta_dict // path: genome.fasta.dict gtf = ch_gtf // path: genome.gtf - gene_bed = ch_gene_bed // path: gene.bed + exon_bed = ch_exon_bed // path: exon.bed star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index fc92918c..14c6d398 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -120,7 +120,7 @@ workflow RNAVAR { // SUBWORKFLOW: Uncompress and prepare reference genome files // PREPARE_GENOME (prepareToolIndices) - ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.gene_bed) + ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.exon_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) // From 27902f769d1b067d3132779aad3cf71930c409fe Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 21 Apr 2022 15:33:03 +0200 Subject: [PATCH 162/235] removed the un-used gtf_gene_filter.nf --- modules/local/gtf_gene_filter.nf | 32 -------------------------------- 1 file changed, 32 deletions(-) delete mode 100644 modules/local/gtf_gene_filter.nf diff --git a/modules/local/gtf_gene_filter.nf b/modules/local/gtf_gene_filter.nf deleted file mode 100644 index f324ace2..00000000 --- a/modules/local/gtf_gene_filter.nf +++ /dev/null @@ -1,32 +0,0 @@ -process GTF_GENE_FILTER { - tag "$fasta" - - conda (params.enable_conda ? "conda-forge::python=3.9.5" : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.9--1' : - 'quay.io/biocontainers/python:3.9--1' }" - - input: - path fasta - path gtf - - output: - path "*.gtf" , emit: gtf - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // filter_gtf_for_genes_in_genome.py is bundled with the pipeline, in nf-core/rnaseq/bin/ - """ - filter_gtf_for_genes_in_genome.py \\ - --gtf $gtf \\ - --fasta $fasta \\ - -o ${fasta.baseName}_genes.gtf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} From 530e8b8b666f51974bf3493dbd59a3c715eb8946 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 22 Apr 2022 12:34:37 +0200 Subject: [PATCH 163/235] Fix: avoid calling container when a local cache is provided for vep or snpeff --- conf/modules.config | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index ba366246..93149685 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -340,13 +340,17 @@ process { withName: 'ENSEMBLVEP' { ext.args = '--everything --filter_common --per_gene --total_length --offline' - container = { params.genome ? "nfcore/vep:104.3.${params.genome}" : "nfcore/vep:104.3.${params.vep_genome}" } + if(!params.vep_cache){ + container = { params.genome ? "nfcore/vep:104.3.${params.genome}" : "nfcore/vep:104.3.${params.vep_genome}" } + } publishDir = [ enabled: false ] } withName: 'SNPEFF' { ext.args = '-nodownload -canon -v' - container = { params.genome ? "nfcore/snpeff:5.0.${params.genome}" : "nfcore/snpeff:5.0.${params.snpeff_db.split('\\.')[0]}" } + if(!params.snpeff_cache){ + container = { params.genome ? "nfcore/snpeff:5.0.${params.genome}" : "nfcore/snpeff:5.0.${params.snpeff_db.split('\\.')[0]}" } + } publishDir = [ enabled: false ] } From 3a007b0232aeb98bdc962749ca3da8b68babd13a Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 22 Apr 2022 13:47:20 +0200 Subject: [PATCH 164/235] Prettier fix; changes in Usage.md; Add: parameter validation in WorkflowRnavar.groovy --- docs/usage.md | 2 +- lib/WorkflowRnavar.groovy | 10 ++++++++++ modules.json | 2 +- 3 files changed, 12 insertions(+), 2 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 4a7c6dc1..accfbfa5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -100,7 +100,7 @@ The index building step can be quite a time-consuming process and it permits the - If `--genome` is provided then the FASTA and GTF files (and existing indices) will be automatically obtained from AWS-iGenomes unless these have already been downloaded locally in the path specified by `--igenomes_base`. - If `--gff` is provided as input then this will be converted to a GTF file, or the latter will be used if both are provided. -- If `--exon_bed` is not provided then it will be generated from the GTF file. +- The `--exon_bed` parameter file is expected to be exon coordinates with at least three columns i.e., in the file. The should be 0-based. If this parameter is not provided, the exon coordinates are extracted from the GTF file and generates a bed file by the process `GTF2BED`. - If `--star_index` is not provided then it will be generated from the reference genome FASTA file using `STAR --runmode genomeGenerate` command. > **NB:** In case if you are providing a GTF and/or a BED file, please ensure that the chromosomes and contigs in the files are also present in the genome FASTA (and in the .dict) file. Otherwise `GATK BedToIntervalList` module is likely to fail if the chromosomes/contigs do not match with the reference genome data. diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 43dd2c30..6aee38d4 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -25,6 +25,16 @@ class WorkflowRnavar { System.exit(1) } + if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('vep')) && (!params.genome || !params.vep_genome)) { + log.error "Either --genome or --vep_genome is required to run VEP variant annotation." + System.exit(1) + } + + if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('snpeff')) && (!params.genome || !params.snpeff_db)) { + log.error "Either --genome or --snpeff_db is required to run snpEff variant annotation." + System.exit(1) + } + } // diff --git a/modules.json b/modules.json index db3c3d1c..209fd9bc 100644 --- a/modules.json +++ b/modules.json @@ -92,4 +92,4 @@ } } } -} \ No newline at end of file +} From 250bc9f0e057402348e990fb8733b32f0472b359 Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 22 Apr 2022 15:53:23 +0200 Subject: [PATCH 165/235] Add: missing paramters in config; parameter validation in WorkflowRnavar.groovy --- lib/WorkflowRnavar.groovy | 4 ++-- nextflow.config | 3 +++ workflows/rnavar.nf | 12 ++++++------ 3 files changed, 11 insertions(+), 8 deletions(-) diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 6aee38d4..a5726597 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -25,8 +25,8 @@ class WorkflowRnavar { System.exit(1) } - if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('vep')) && (!params.genome || !params.vep_genome)) { - log.error "Either --genome or --vep_genome is required to run VEP variant annotation." + if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('vep')) && (!params.genome || !params.vep_genome || !params.vep_species || !params.vep_cache_version)) { + log.error "Species name (using --vep_species), genome assembly (either --genome or --vep_genome) and cache version (--vep_cache_version) are required to run VEP variant annotation." System.exit(1) } diff --git a/nextflow.config b/nextflow.config index d7cbe944..e6459470 100644 --- a/nextflow.config +++ b/nextflow.config @@ -53,7 +53,10 @@ params { cadd_wg_snvs_tbi = null // No CADD SNVs index genesplicer = null // genesplicer disabled within VEP snpeff_cache = null // No directory for snpEff cache + snpeff_db = null // No default db for snpeff vep_cache = null // No directory for VEP cache + vep_genome = null // No default genome for VEP + vep_cache_version = null // No default cache version for VEP // Skip steps skip_baserecalibration = false diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 14c6d398..08f453c0 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -96,12 +96,12 @@ dbsnp = params.dbsnp ? Channel.fromPath(params.dbs dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : [] // Initialize varaint annotation associated channels -def snpeff_db = params.snpeff_db ?: Channel.empty() -def vep_cache_version = params.vep_cache_version ?: Channel.empty() -def vep_genome = params.vep_genome ?: Channel.empty() -def vep_species = params.vep_species ?: Channel.empty() -def snpeff_cache = params.snpeff_cache ? params.snpeff_cache : [] -def vep_cache = params.vep_cache ? params.vep_cache : [] +def snpeff_db = params.snpeff_db ?: Channel.empty() +def vep_cache_version = params.vep_cache_version ?: Channel.empty() +def vep_genome = params.vep_genome ?: Channel.empty() +def vep_species = params.vep_species ?: Channel.empty() +def snpeff_cache = params.snpeff_cache ? Channel.fromPath(params.snpeff_cache).collect() : [] +def vep_cache = params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : [] // MultiQC reporting def multiqc_report = [] From d1d5fc5085602e10900778ff39775e2fb2532b38 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 28 Apr 2022 15:28:44 +0200 Subject: [PATCH 166/235] Fix: use of csi index when work with large genomes and --bam_csi_index --- conf/modules.config | 21 +++++++-- modules.json | 13 +++--- modules/nf-core/modules/cat/fastq/main.nf | 4 +- .../modules/gatk4/haplotypecaller/main.nf | 2 +- modules/nf-core/modules/gunzip/main.nf | 4 +- modules/nf-core/modules/tabix/tabix/main.nf | 42 +++++++++++++++++ modules/nf-core/modules/tabix/tabix/meta.yml | 45 +++++++++++++++++++ modules/nf-core/modules/untar/main.nf | 6 +-- subworkflows/local/splitncigar.nf | 8 +++- subworkflows/nf-core/markduplicates.nf | 1 + subworkflows/nf-core/recalibrate.nf | 9 +++- workflows/rnavar.nf | 22 +++++---- 12 files changed, 151 insertions(+), 26 deletions(-) create mode 100644 modules/nf-core/modules/tabix/tabix/main.nf create mode 100644 modules/nf-core/modules/tabix/tabix/meta.yml diff --git a/conf/modules.config b/conf/modules.config index 93149685..5abd0ef2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -237,10 +237,22 @@ process { ] } + withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:GATK4_SPLITNCIGARREADS' { + ext.args = '--create-output-bam-index false' + } + + withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + } + withName: '.*:SPLITNCIGAR:.*' { publishDir = [ enabled: false ] } + withName: '.*:SPLITNCIGAR:SAMTOOLS_INDEX' { + ext.args = params.bam_csi_index ? '-c' : '' + } + withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { publishDir = [ path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, @@ -295,13 +307,15 @@ process { withName: GATK4_HAPLOTYPECALLER { ext.args = [ '-dont-use-soft-clipped-bases', - params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '' + params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '', + params.bam_csi_index ? "--create-output-variant-index false" : "" ].join(' ').trim() publishDir = [ enabled: false ] } withName: GATK4_MERGEVCFS { ext.prefix = {"${meta.id}.haplotypecaller"} + ext.args = params.bam_csi_index ? '--CREATE_INDEX false' : '' publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, @@ -309,12 +323,13 @@ process { ] } - withName: GATK4_INDEXFEATUREFILE { + withName: TABIX_TABIX { + ext.args = params.bam_csi_index ? '--csi' : '' ext.prefix = {"${meta.id}.haplotypecaller"} publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, - pattern: "*.{vcf.gz.tbi}" + pattern: "*.{vcf.gz.tbi,vcf.gz.csi}" ] } diff --git a/modules.json b/modules.json index 209fd9bc..014d4f85 100644 --- a/modules.json +++ b/modules.json @@ -4,7 +4,7 @@ "repos": { "nf-core/modules": { "cat/fastq": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "9aadd9a6d3f5964476582319b3a1c54a3e3fe7c9" }, "custom/dumpsoftwareversions": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" @@ -28,7 +28,7 @@ "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, "gatk4/haplotypecaller": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "68f1c27169946f931ea4318911de5681f88b2961" }, "gatk4/indexfeaturefile": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" @@ -46,7 +46,7 @@ "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" }, "gunzip": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "9aadd9a6d3f5964476582319b3a1c54a3e3fe7c9" }, "multiqc": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" @@ -87,9 +87,12 @@ "tabix/bgziptabix": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, + "tabix/tabix": { + "git_sha": "b3e9b88e80880f450ad79a95b2b7aa05e1de5484" + }, "untar": { - "git_sha": "9ae34a01d1747019fd37753ff4cafb05aec35a2b" + "git_sha": "9aadd9a6d3f5964476582319b3a1c54a3e3fe7c9" } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/cat/fastq/main.nf b/modules/nf-core/modules/cat/fastq/main.nf index bf0877c3..b6854895 100644 --- a/modules/nf-core/modules/cat/fastq/main.nf +++ b/modules/nf-core/modules/cat/fastq/main.nf @@ -4,8 +4,8 @@ process CAT_FASTQ { conda (params.enable_conda ? "conda-forge::sed=4.7" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : - 'biocontainers/biocontainers:v1.2.0_cv1' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'ubuntu:20.04' }" input: tuple val(meta), path(reads, stageAs: "input*/*") diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf index 57f69ecd..2cd9e7d4 100644 --- a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf +++ b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf @@ -17,7 +17,7 @@ process GATK4_HAPLOTYPECALLER { output: tuple val(meta), path("*.vcf.gz"), emit: vcf - tuple val(meta), path("*.tbi") , emit: tbi + tuple val(meta), path("*.tbi") , optional:true, emit: tbi path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/modules/gunzip/main.nf index 9d4b0666..61bf1afa 100644 --- a/modules/nf-core/modules/gunzip/main.nf +++ b/modules/nf-core/modules/gunzip/main.nf @@ -4,8 +4,8 @@ process GUNZIP { conda (params.enable_conda ? "conda-forge::sed=4.7" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' : - 'biocontainers/biocontainers:v1.2.0_cv1' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'ubuntu:20.04' }" input: tuple val(meta), path(archive) diff --git a/modules/nf-core/modules/tabix/tabix/main.nf b/modules/nf-core/modules/tabix/tabix/main.nf new file mode 100644 index 00000000..e155e468 --- /dev/null +++ b/modules/nf-core/modules/tabix/tabix/main.nf @@ -0,0 +1,42 @@ +process TABIX_TABIX { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? 'bioconda::tabix=1.11' : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(tab) + + output: + tuple val(meta), path("*.tbi"), optional:true, emit: tbi + tuple val(meta), path("*.csi"), optional:true, emit: csi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + tabix $args $tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${tab}.tbi + cat <<-END_VERSIONS > versions.yml + + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/tabix/tabix/meta.yml b/modules/nf-core/modules/tabix/tabix/meta.yml new file mode 100644 index 00000000..fcc6e524 --- /dev/null +++ b/modules/nf-core/modules/tabix/tabix/meta.yml @@ -0,0 +1,45 @@ +name: tabix_tabix +description: create tabix index from a sorted bgzip tab-delimited genome file +keywords: + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file compressed with bgzip + pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tbi: + type: file + description: tabix index file + pattern: "*.{tbi}" + - csi: + type: file + description: coordinate sorted index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/modules/untar/main.nf index bbfa0bfe..058d1764 100644 --- a/modules/nf-core/modules/untar/main.nf +++ b/modules/nf-core/modules/untar/main.nf @@ -2,10 +2,10 @@ process UNTAR { tag "$archive" label 'process_low' - conda (params.enable_conda ? "conda-forge::tar=1.34" : null) + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv2/biocontainers_v1.2.0_cv2.img' : - 'biocontainers/biocontainers:v1.2.0_cv2' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'ubuntu:20.04' }" input: tuple val(meta), path(archive) diff --git a/subworkflows/local/splitncigar.nf b/subworkflows/local/splitncigar.nf index db2dd73a..d16ee9a1 100644 --- a/subworkflows/local/splitncigar.nf +++ b/subworkflows/local/splitncigar.nf @@ -45,7 +45,13 @@ workflow SPLITNCIGAR { ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) SAMTOOLS_INDEX(splitncigar_bam) - splitncigar_bam_bai = splitncigar_bam.join(SAMTOOLS_INDEX.out.bai) + splitncigar_bam_bai = splitncigar_bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map{meta, bam, bai, csi -> + if (bai) [meta, bam, bai] + else [meta, bam, csi] + } ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) emit: diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index 482b1889..3a10551f 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -40,6 +40,7 @@ workflow MARKDUPLICATES { emit: bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + bam_bai = ch_bam_bai // channel: [ val(meta), [ bam ], [bai or csi] ] metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 7c6e4fe2..17e1e977 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -35,7 +35,14 @@ workflow RECALIBRATE { ch_versions = ch_versions.mix(APPLYBQSR.out.versions.first()) SAMTOOLS_INDEX(bam_recalibrated) - bam_recalibrated_index = bam_recalibrated.join(SAMTOOLS_INDEX.out.bai) + bam_recalibrated_index = bam_recalibrated + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map{meta, bam, bai, csi -> + if (bai) [meta, bam, bai] + else [meta, bam, csi] + } + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) samtools_stats = Channel.empty() diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 08f453c0..12230916 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -68,6 +68,7 @@ include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4 include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' +include { TABIX_TABIX as TABIX } from '../modules/nf-core/modules/tabix/tabix/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' /* @@ -214,17 +215,15 @@ workflow RNAVAR { ch_samtools_flagstat = ALIGN_STAR.out.flagstat ch_samtools_idxstats = ALIGN_STAR.out.idxstats ch_star_multiqc = ALIGN_STAR.out.log_final - if (params.bam_csi_index) ch_genome_bam_index = ALIGN_STAR.out.csi ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) // SUBWORKFLOW: Mark duplicates with Picard MARKDUPLICATES(ch_genome_bam) - ch_genome_bam = MARKDUPLICATES.out.bam.join(MARKDUPLICATES.out.bai, by: [0]) + ch_genome_bam = MARKDUPLICATES.out.bam_bai ch_samtools_stats = MARKDUPLICATES.out.stats ch_samtools_flagstat = MARKDUPLICATES.out.flagstat ch_samtools_idxstats = MARKDUPLICATES.out.idxstats ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics - if (params.bam_csi_index) ch_genome_bam_index = MARKDUPLICATES.out.csi ch_versions = ch_versions.mix(MARKDUPLICATES.out.versions.first().ifEmpty(null)) // Subworkflow - SplitNCigarReads from GATK4 over the intervals @@ -319,12 +318,19 @@ workflow RNAVAR { haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) - GATK4_INDEXFEATUREFILE( + TABIX( haplotypecaller_vcf ) - haplotypecaller_vcf = haplotypecaller_vcf //.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) - haplotypecaller_vcf_tbi = haplotypecaller_vcf.join(GATK4_INDEXFEATUREFILE.out.index, by: [0]) - ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) + + haplotypecaller_vcf_tbi = haplotypecaller_vcf + .join(TABIX.out.tbi, by: [0], remainder: true) + .join(TABIX.out.csi, by: [0], remainder: true) + .map{meta, vcf, tbi, csi -> + if (tbi) [meta, vcf, tbi] + else [meta, vcf, csi] + } + + ch_versions = ch_versions.mix(TABIX.out.versions.first().ifEmpty(null)) final_vcf = haplotypecaller_vcf // MODULE: VariantFiltration from GATK4 @@ -337,7 +343,7 @@ workflow RNAVAR { PREPARE_GENOME.out.dict ) - filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf //.join(GATK4_VARIANTFILTRATION.out.tbi, by: [0]) + filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf final_vcf = filtered_vcf ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) } From 756afe1050d165f4082d5877de32188071520e85 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 28 Apr 2022 15:55:06 +0200 Subject: [PATCH 167/235] update: doc for large genome and use of --bam_csi_index --- docs/usage.md | 6 ++++++ workflows/rnavar.nf | 2 +- 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index accfbfa5..6faf9dbe 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -105,6 +105,12 @@ The index building step can be quite a time-consuming process and it permits the > **NB:** In case if you are providing a GTF and/or a BED file, please ensure that the chromosomes and contigs in the files are also present in the genome FASTA (and in the .dict) file. Otherwise `GATK BedToIntervalList` module is likely to fail if the chromosomes/contigs do not match with the reference genome data. +### Recommendation when using very large genomes + +When the pipeline is used on very large genomes having chromosome size greater than 512Mb (e.g. Chromosome 1 of Monodelphis domestica has a size of 748055161bp), please make sure that `--bam_csi_index` parameter is provided in order to use coordinate sorted index (CSI) instead of standard binary alignment index (BAI). + +> **NB:** When `--bam_csi_index` is used, variant filtration step will be disabled as `GATK VariantFiltration` does not currently support CSI index for the input VCF. It may be incorporated in the future when newer GATK versions support CSI for VCF inputs. + ## Alignment options The pipeline uses [STAR](https://github.com/alexdobin/STAR) to map the raw FastQ reads to the reference genome. STAR is fast but requires a lot of memory to run, typically around 38GB for the Human GRCh37 reference genome. diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 12230916..0efab079 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -334,7 +334,7 @@ workflow RNAVAR { final_vcf = haplotypecaller_vcf // MODULE: VariantFiltration from GATK4 - if (!params.skip_variantfiltration) { + if (!params.skip_variantfiltration && !params.bam_csi_index ) { GATK4_VARIANTFILTRATION( haplotypecaller_vcf_tbi, From 93041079b70b0a2c495730d0733b73d9d178f760 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 28 Apr 2022 16:01:20 +0200 Subject: [PATCH 168/235] Used nf-core version of gatk4/splitncigarreads --- modules.json | 3 +++ .../modules}/gatk4/splitncigarreads/main.nf | 14 ++++++++------ .../modules}/gatk4/splitncigarreads/meta.yml | 10 +++++----- subworkflows/nf-core/splitn_cigar_reads.nf | 4 ++-- 4 files changed, 18 insertions(+), 13 deletions(-) rename modules/{local => nf-core/modules}/gatk4/splitncigarreads/main.nf (81%) rename modules/{local => nf-core/modules}/gatk4/splitncigarreads/meta.yml (91%) diff --git a/modules.json b/modules.json index 014d4f85..e86ffbf1 100644 --- a/modules.json +++ b/modules.json @@ -39,6 +39,9 @@ "gatk4/mergevcfs": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, + "gatk4/splitncigarreads": { + "git_sha": "10ca39a86f1b227a5535238d02398fec686eba72" + }, "gatk4/variantfiltration": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, diff --git a/modules/local/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf similarity index 81% rename from modules/local/gatk4/splitncigarreads/main.nf rename to modules/nf-core/modules/gatk4/splitncigarreads/main.nf index 69531167..85e5daa8 100644 --- a/modules/local/gatk4/splitncigarreads/main.nf +++ b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf @@ -8,7 +8,7 @@ process GATK4_SPLITNCIGARREADS { 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" input: - tuple val(meta), path(bam), path(bai), path(interval) + tuple val(meta), path(bam), path(bai), path(intervals) path fasta path fai path dict @@ -23,7 +23,8 @@ process GATK4_SPLITNCIGARREADS { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def interval_option = interval ? "-L ${interval}" : "" + def interval_command = intervals ? "--intervals $intervals" : "" + def avail_mem = 3 if (!task.memory) { log.info '[GATK SplitNCigarReads] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' @@ -32,10 +33,11 @@ process GATK4_SPLITNCIGARREADS { } """ gatk --java-options "-Xmx${avail_mem}g" SplitNCigarReads \\ - -R $fasta \\ - -I $bam \\ - ${interval_option} \\ - -O ${prefix}.bam \\ + --input $bam \\ + --output ${prefix}.bam \\ + --reference $fasta \\ + $interval_command \\ + --tmp-dir . \\ $args cat <<-END_VERSIONS > versions.yml diff --git a/modules/local/gatk4/splitncigarreads/meta.yml b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml similarity index 91% rename from modules/local/gatk4/splitncigarreads/meta.yml rename to modules/nf-core/modules/gatk4/splitncigarreads/meta.yml index c26e9aa8..76bfdcd3 100644 --- a/modules/local/gatk4/splitncigarreads/meta.yml +++ b/modules/nf-core/modules/gatk4/splitncigarreads/meta.yml @@ -24,12 +24,12 @@ input: description: BAM/SAM/CRAM file containing reads pattern: "*.{bam,sam,cram}" - bai: + type: list + description: BAI/SAI/CRAI index file (optional) + pattern: "*.{bai,sai,crai}" + - intervals: type: file - description: BAI/CRAI file from alignment - pattern: "*.{bai,crai}" - - interval: - type: file - description: Bed file with the genomic regions included in the library + description: Bed file with the genomic regions included in the library (optional) - fasta: type: file description: The reference fasta file diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf index 4e63c322..ef2fd253 100644 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ b/subworkflows/nf-core/splitn_cigar_reads.nf @@ -6,8 +6,8 @@ //params.samtools_index_options = [:] //params.samtools_stats_options = [:] -include { GATK4_SPLITNCIGARREADS } from '../../modules/local/gatk4/splitncigarreads/main' //addParams( options: params.gatk_splitncigar_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.samtools_index_options ) +include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' //addParams( options: params.gatk_splitncigar_options ) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.samtools_index_options ) workflow GATK4_SPLITNCIGAR { take: From 148466a6e2a9592005b2a0cbbd40773c5124c6a0 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 28 Apr 2022 16:14:34 +0200 Subject: [PATCH 169/235] Fix: issues with known variants VCF resources --- lib/WorkflowRnavar.groovy | 4 ++-- workflows/rnavar.nf | 15 ++++++++++----- 2 files changed, 12 insertions(+), 7 deletions(-) diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index a5726597..537f2f52 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -20,8 +20,8 @@ class WorkflowRnavar { System.exit(1) } - if ((!params.skip_baserecalibration) && (!params.known_indels || !params.known_indels_tbi)) { - log.error "Known variants VCF file or its index is missing!. Use --known_indels and --known_indels_tbi to supply the files." + if ((!params.skip_baserecalibration) && (!params.dbsnp || !params.known_indels)) { + log.error "Known variants VCF file or its index is missing!. Atleast --dbsnp (and its index) or --known_indels (and its index) is required." System.exit(1) } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 0efab079..0a7e42d1 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -93,8 +93,10 @@ def seq_platform = params.seq_platform ? params.seq_platform : [] def seq_center = params.seq_center ? params.seq_center : [] // Initialize file channels based on params -dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : [] -dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : [] +dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : Channel.empty() +dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : Channel.empty() +known_sites = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() +known_sites_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() // Initialize varaint annotation associated channels def snpeff_db = params.snpeff_db ?: Channel.empty() @@ -237,9 +239,12 @@ workflow RNAVAR { if(!params.skip_baserecalibration) { // MODULE: BaseRecalibrator from GATK4 - ch_bqsr_table = Channel.empty() - known_sites = Channel.from([params.dbsnp, params.known_indels]).collect() - known_sites_tbi = Channel.from([params.dbsnp_tbi, params.known_indels_tbi]).collect() + ch_bqsr_table = Channel.empty() + + // known_sites is made by grouping both the dbsnp and the known indels ressources + // they can either or both be optional + known_sites = dbsnp.concat(known_indels).collect() + known_sites_tbi = dbsnp_tbi.concat(known_indels_tbi).collect() ch_interval_list_recalib = ch_interval_list.map{ meta, bed -> [bed] }.flatten() splitncigar_bam_bai.combine(ch_interval_list_recalib) From d0f6f005d624a8a03a269983912567807d02e4af Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 28 Apr 2022 16:16:32 +0200 Subject: [PATCH 170/235] Fix: known indels --- workflows/rnavar.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 0a7e42d1..b79cc193 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -95,8 +95,8 @@ def seq_center = params.seq_center ? params.seq_center : [] // Initialize file channels based on params dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : Channel.empty() dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : Channel.empty() -known_sites = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() -known_sites_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() +known_indels = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() +known_indels_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() // Initialize varaint annotation associated channels def snpeff_db = params.snpeff_db ?: Channel.empty() From e9c1e661a69d439e70fcc80eddd71b927a73e0ed Mon Sep 17 00:00:00 2001 From: Praveen Date: Fri, 29 Apr 2022 15:24:00 +0200 Subject: [PATCH 171/235] Fix: usage of known resources correctly. --- lib/WorkflowRnavar.groovy | 2 +- workflows/rnavar.nf | 6 ++++++ 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 537f2f52..5d19f29f 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -20,7 +20,7 @@ class WorkflowRnavar { System.exit(1) } - if ((!params.skip_baserecalibration) && (!params.dbsnp || !params.known_indels)) { + if ((!params.skip_baserecalibration) && (!params.dbsnp && !params.known_indels)) { log.error "Known variants VCF file or its index is missing!. Atleast --dbsnp (and its index) or --known_indels (and its index) is required." System.exit(1) } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index b79cc193..9e909447 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -289,6 +289,12 @@ workflow RNAVAR { // MODULE: HaplotypeCaller from GATK4 interval_flag = params.no_intervals + // Run haplotyper even in the absence of dbSNP files + if (!params.dbsnp){ + dbsnp = [] + dbsnp_tbi = [] + } + haplotypecaller_vcf = Channel.empty() haplotypecaller_interval_bam = bam_variant_calling.combine(ch_interval_list_split) From d2a9da7c97289e5024471e98c7c04ecce6bbf3f9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 2 May 2022 10:22:48 +0200 Subject: [PATCH 172/235] Fix: Editorconf and prettier issues --- conf/modules.config | 6 +++--- modules.json | 2 +- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 5abd0ef2..c34505bc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -238,7 +238,7 @@ process { } withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:GATK4_SPLITNCIGARREADS' { - ext.args = '--create-output-bam-index false' + ext.args = '--create-output-bam-index false' } withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:SAMTOOLS_INDEX' { @@ -314,8 +314,8 @@ process { } withName: GATK4_MERGEVCFS { - ext.prefix = {"${meta.id}.haplotypecaller"} - ext.args = params.bam_csi_index ? '--CREATE_INDEX false' : '' + ext.prefix = {"${meta.id}.haplotypecaller"} + ext.args = params.bam_csi_index ? '--CREATE_INDEX false' : '' publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, mode: params.publish_dir_mode, diff --git a/modules.json b/modules.json index e86ffbf1..3133dae3 100644 --- a/modules.json +++ b/modules.json @@ -98,4 +98,4 @@ } } } -} \ No newline at end of file +} From d10e930fd8129cf1fde569aafa8c9682f18f1ba1 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 2 May 2022 10:25:00 +0200 Subject: [PATCH 173/235] Fix: Editorconf issue on modules.config --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index c34505bc..c710bd8b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -228,7 +228,7 @@ process { withName: '.*:MARKDUPLICATES:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' - ext.prefix = {"${meta.id}.markdup.sorted"} + ext.prefix = {"${meta.id}.markdup.sorted"} publishDir = [ path: { "${params.outdir}/preprocessing/${meta.id}" }, mode: params.publish_dir_mode, @@ -238,7 +238,7 @@ process { } withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:GATK4_SPLITNCIGARREADS' { - ext.args = '--create-output-bam-index false' + ext.args = '--create-output-bam-index false' } withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:SAMTOOLS_INDEX' { From fb8f4551f3bc5331e5ac62fb920ee7eacc684d27 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 2 May 2022 12:01:26 +0200 Subject: [PATCH 174/235] Used GATK4/markduplicates command instead of Picard/Markduplicate. Just to standardize. --- conf/modules.config | 8 +-- modules.json | 8 +-- .../modules/gatk4/markduplicates/main.nf | 46 +++++++++++++++ .../modules/gatk4/markduplicates/meta.yml | 52 +++++++++++++++++ .../modules/picard/markduplicates/main.nf | 58 ------------------- .../modules/picard/markduplicates/meta.yml | 52 ----------------- subworkflows/nf-core/markduplicates.nf | 16 ++--- 7 files changed, 114 insertions(+), 126 deletions(-) create mode 100644 modules/nf-core/modules/gatk4/markduplicates/main.nf create mode 100644 modules/nf-core/modules/gatk4/markduplicates/meta.yml delete mode 100644 modules/nf-core/modules/picard/markduplicates/main.nf delete mode 100644 modules/nf-core/modules/picard/markduplicates/meta.yml diff --git a/conf/modules.config b/conf/modules.config index c710bd8b..7622e5b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -211,11 +211,11 @@ process { publishDir = [ enabled: false ] } - withName: '.*:MARKDUPLICATES:PICARD_MARKDUPLICATES' { + withName: '.*:MARKDUPLICATES:GATK4_MARKDUPLICATES' { ext.args = [ - 'ASSUME_SORTED=true', - 'VALIDATION_STRINGENCY=LENIENT', - params.remove_duplicates ? 'REMOVE_DUPLICATES=true' : '' + '--ASSUME_SORTED true', + '--VALIDATION_STRINGENCY LENIENT', + params.remove_duplicates ? '--REMOVE_DUPLICATES true' : '' ].join(' ').trim() ext.prefix = {"${meta.id}.markdup.sorted"} publishDir = [ diff --git a/modules.json b/modules.json index 3133dae3..61e0b776 100644 --- a/modules.json +++ b/modules.json @@ -36,6 +36,9 @@ "gatk4/intervallisttools": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, + "gatk4/markduplicates": { + "git_sha": "0511e7fbbfa4ba41940d33b687b1cc90227b4eb8" + }, "gatk4/mergevcfs": { "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" }, @@ -54,9 +57,6 @@ "multiqc": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, - "picard/markduplicates": { - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" - }, "samtools/faidx": { "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, @@ -98,4 +98,4 @@ } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/modules/gatk4/markduplicates/main.nf b/modules/nf-core/modules/gatk4/markduplicates/main.nf new file mode 100644 index 00000000..2650925b --- /dev/null +++ b/modules/nf-core/modules/gatk4/markduplicates/main.nf @@ -0,0 +1,46 @@ +process GATK4_MARKDUPLICATES { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : + 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + + input: + tuple val(meta), path(bam) + + output: + tuple val(meta), path("*.bam") , emit: bam + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.metrics"), emit: metrics + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def input_list = bam.collect{"--INPUT $it"}.join(' ') + + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk --java-options "-Xmx${avail_mem}g" MarkDuplicates \\ + $input_list \\ + --OUTPUT ${prefix}.bam \\ + --METRICS_FILE ${prefix}.metrics \\ + --TMP_DIR . \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/markduplicates/meta.yml b/modules/nf-core/modules/gatk4/markduplicates/meta.yml new file mode 100644 index 00000000..93877f47 --- /dev/null +++ b/modules/nf-core/modules/gatk4/markduplicates/meta.yml @@ -0,0 +1,52 @@ +name: gatk4_markduplicates +description: This tool locates and tags duplicate reads in a BAM or SAM file, where duplicate reads are defined as originating from a single fragment of DNA. +keywords: + - markduplicates + - bam + - sort +tools: + - gatk4: + description: + Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard- + tool_dev_url: https://github.com/broadinstitute/gatk + doi: 10.1158/1538-7445.AM2017-3590 + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: Sorted BAM file + pattern: "*.{bam}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - bam: + type: file + description: Marked duplicates BAM file + pattern: "*.{bam}" + - metrics: + type: file + description: Duplicate metrics file generated by GATK + pattern: "*.{metrics.txt}" + +authors: + - "@ajodeh-juma" + - "@FriederikeHanssen" + - "@maxulysse" diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/modules/picard/markduplicates/main.nf deleted file mode 100644 index e754a587..00000000 --- a/modules/nf-core/modules/picard/markduplicates/main.nf +++ /dev/null @@ -1,58 +0,0 @@ -process PICARD_MARKDUPLICATES { - tag "$meta.id" - label 'process_medium' - - conda (params.enable_conda ? "bioconda::picard=2.26.10" : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' : - 'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }" - - input: - tuple val(meta), path(bam) - - output: - tuple val(meta), path("*.bam") , emit: bam - tuple val(meta), path("*.bai") , optional:true, emit: bai - tuple val(meta), path("*.metrics.txt"), emit: metrics - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def avail_mem = 3 - if (!task.memory) { - log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' - } else { - avail_mem = task.memory.giga - } - """ - picard \\ - -Xmx${avail_mem}g \\ - MarkDuplicates \\ - $args \\ - I=$bam \\ - O=${prefix}.bam \\ - M=${prefix}.MarkDuplicates.metrics.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bam - touch ${prefix}.bam.bai - touch ${prefix}.MarkDuplicates.metrics.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - picard: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) - END_VERSIONS - """ -} diff --git a/modules/nf-core/modules/picard/markduplicates/meta.yml b/modules/nf-core/modules/picard/markduplicates/meta.yml deleted file mode 100644 index 842817bc..00000000 --- a/modules/nf-core/modules/picard/markduplicates/meta.yml +++ /dev/null @@ -1,52 +0,0 @@ -name: picard_markduplicates -description: Locate and tag duplicate reads in a BAM file -keywords: - - markduplicates - - pcr - - duplicates - - bam - - sam - - cram -tools: - - picard: - description: | - A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) - data and formats such as SAM/BAM/CRAM and VCF. - homepage: https://broadinstitute.github.io/picard/ - documentation: https://broadinstitute.github.io/picard/ - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file - pattern: "*.{bam}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bam: - type: file - description: BAM file with duplicate reads marked/removed - pattern: "*.{bam}" - - bai: - type: file - description: An optional BAM index file. If desired, --CREATE_INDEX must be passed as a flag - pattern: "*.{bai}" - - metrics: - type: file - description: Duplicate metrics file generated by picard - pattern: "*.{metrics.txt}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@projectoriented" diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index 3a10551f..d9b4df2b 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -1,5 +1,5 @@ // -// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// GATK4 MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats // //params.markduplicates_options = [:] @@ -7,7 +7,7 @@ //params.samtools_stats_options = [:] include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' //addParams(options: params.samtools_stats_options) -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' //addParams(options: params.markduplicates_options) +include { GATK4_MARKDUPLICATES } from '../../modules/nf-core/modules/gatk4/markduplicates/main' //addParams(options: params.markduplicates_options) include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) workflow MARKDUPLICATES { @@ -18,16 +18,16 @@ workflow MARKDUPLICATES { ch_versions = Channel.empty() - PICARD_MARKDUPLICATES(bam) - ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) + GATK4_MARKDUPLICATES(bam) + ch_versions = ch_versions.mix(GATK4_MARKDUPLICATES.out.versions.first()) // // Index BAM file and run samtools stats, flagstat and idxstats // - SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) + SAMTOOLS_INDEX(GATK4_MARKDUPLICATES.out.bam) ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - PICARD_MARKDUPLICATES.out.bam + GATK4_MARKDUPLICATES.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) .map{meta, bam, bai, csi -> @@ -39,9 +39,9 @@ workflow MARKDUPLICATES { ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) emit: - bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] + bam = GATK4_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] bam_bai = ch_bam_bai // channel: [ val(meta), [ bam ], [bai or csi] ] - metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] + metrics = GATK4_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] From bc55a49c87c615ae6114db102e211c6fb51d5740 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 2 May 2022 12:11:12 +0200 Subject: [PATCH 175/235] Prettier fix --- modules.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules.json b/modules.json index 61e0b776..3f7997cc 100644 --- a/modules.json +++ b/modules.json @@ -98,4 +98,4 @@ } } } -} \ No newline at end of file +} From 5ef0c510667b9c7b4062c0ed06c026fe6ccfa085 Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Mon, 2 May 2022 12:56:31 +0200 Subject: [PATCH 176/235] Update lib/WorkflowRnavar.groovy Co-authored-by: Maxime U. Garcia --- lib/WorkflowRnavar.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/WorkflowRnavar.groovy b/lib/WorkflowRnavar.groovy index 5d19f29f..9f530eb3 100755 --- a/lib/WorkflowRnavar.groovy +++ b/lib/WorkflowRnavar.groovy @@ -21,7 +21,7 @@ class WorkflowRnavar { } if ((!params.skip_baserecalibration) && (!params.dbsnp && !params.known_indels)) { - log.error "Known variants VCF file or its index is missing!. Atleast --dbsnp (and its index) or --known_indels (and its index) is required." + log.error "Known variants VCF file or its index is missing!. At least --dbsnp (and its index) or --known_indels (and its index) is required." System.exit(1) } From a06f3909ac7255c20da3b0fbfa36ee2cdedb346d Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 3 May 2022 15:47:28 +0200 Subject: [PATCH 177/235] Simplified splitNCigar subworkflow; Fix: bam_csi_index; added test for bam_csi_index --- .github/workflows/ci.yml | 1 + conf/modules.config | 8 +-- subworkflows/nf-core/splitn_cigar_reads.nf | 54 ------------------- .../{local => nf-core}/splitncigar.nf | 18 +++---- tests/test_bamcsiindex.yml | 14 +++++ workflows/rnavar.nf | 2 +- 6 files changed, 25 insertions(+), 72 deletions(-) delete mode 100644 subworkflows/nf-core/splitn_cigar_reads.nf rename subworkflows/{local => nf-core}/splitncigar.nf (61%) create mode 100644 tests/test_bamcsiindex.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 1eb793b1..dbe11020 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -33,6 +33,7 @@ jobs: - "annotation" - "removeduplicates" - "skipbasecalib" + - "bamcsiindex" steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/conf/modules.config b/conf/modules.config index 7622e5b6..df225d23 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -237,11 +237,11 @@ process { ] } - withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:GATK4_SPLITNCIGARREADS' { + withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGARREADS' { ext.args = '--create-output-bam-index false' } - withName: '.*:SPLITNCIGAR:GATK4_SPLITNCIGAR:SAMTOOLS_INDEX' { + withName: '.*:SPLITNCIGAR:SAMTOOLS_INDEX' { ext.args = params.bam_csi_index ? '-c' : '' } @@ -249,10 +249,6 @@ process { publishDir = [ enabled: false ] } - withName: '.*:SPLITNCIGAR:SAMTOOLS_INDEX' { - ext.args = params.bam_csi_index ? '-c' : '' - } - withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { publishDir = [ path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, diff --git a/subworkflows/nf-core/splitn_cigar_reads.nf b/subworkflows/nf-core/splitn_cigar_reads.nf deleted file mode 100644 index ef2fd253..00000000 --- a/subworkflows/nf-core/splitn_cigar_reads.nf +++ /dev/null @@ -1,54 +0,0 @@ -// -// GATK4 SplitNCigarReads -// - -//params.gatk_splitncigar_options = [:] -//params.samtools_index_options = [:] -//params.samtools_stats_options = [:] - -include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' //addParams( options: params.gatk_splitncigar_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.samtools_index_options ) - -workflow GATK4_SPLITNCIGAR { - take: - bam_interval // channel: [ val(meta), [ bam ], [bai], [interval] ] - fasta // channel: [ fasta ] - fasta_fai // channel: [ fai ] - fasta_dict // channel: [ dict ] - - main: - - ch_versions = Channel.empty() - - // - // GATK4 - // - GATK4_SPLITNCIGARREADS ( bam_interval, fasta, fasta_fai, fasta_dict) - ch_versions = ch_versions.mix(GATK4_SPLITNCIGARREADS.out.versions.first()) - - // - // Index BAM file and run samtools stats, flagstat and idxstats - // - SAMTOOLS_INDEX ( GATK4_SPLITNCIGARREADS.out.bam ) - ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - - GATK4_SPLITNCIGARREADS.out.bam - .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) - .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map { - meta, bam, bai, csi -> - if (bai) { - [ meta, bam, bai ] - } else { - [ meta, bam, csi ] - } - } - .set { ch_bam_bai } - - - emit: - bam = GATK4_SPLITNCIGARREADS.out.bam // channel: [ val(meta), [ bam ] ] - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/local/splitncigar.nf b/subworkflows/nf-core/splitncigar.nf similarity index 61% rename from subworkflows/local/splitncigar.nf rename to subworkflows/nf-core/splitncigar.nf index d16ee9a1..6f941a35 100644 --- a/subworkflows/local/splitncigar.nf +++ b/subworkflows/nf-core/splitncigar.nf @@ -1,14 +1,10 @@ // -// PREPROCESSING OF ALIGNMENT FILE OVER THE INTERVALS IN PARALLEL +// Subworkflow: Run GATK4 SplitNCigarReads with intervals, merge and index BAM file. // -//params.gatk_splitncigar_options = [:] -//params.samtools_index_options = [:] -//params.samtools_merge_options = [:] - -include { GATK4_SPLITNCIGAR } from '../nf-core/splitn_cigar_reads' //addParams(gatk_splitncigar_options: params.gatk_splitncigar_options, samtools_index_options: params.samtools_index_options) -include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' //addParams(options: params.samtools_merge_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) +include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' //addParams(options: params.samtools_merge_options) +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) workflow SPLITNCIGAR { take: @@ -30,9 +26,9 @@ workflow SPLITNCIGAR { [new_meta, bam, bai, intervals] }.set{bam_interval} - GATK4_SPLITNCIGAR(bam_interval, fasta, fasta_fai, fasta_dict) - bam_splitncigar = GATK4_SPLITNCIGAR.out.bam - ch_versions = ch_versions.mix(GATK4_SPLITNCIGAR.out.versions.first()) + GATK4_SPLITNCIGARREADS(bam_interval, fasta, fasta_fai, fasta_dict) + bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam + ch_versions = ch_versions.mix(GATK4_SPLITNCIGARREADS.out.versions.first()) bam_splitncigar .map{ meta, bam -> diff --git a/tests/test_bamcsiindex.yml b/tests/test_bamcsiindex.yml new file mode 100644 index 00000000..e1fd170e --- /dev/null +++ b/tests/test_bamcsiindex.yml @@ -0,0 +1,14 @@ +- name: Run pipeline with bam_csi_index for large genomes + command: nextflow run main.nf -profile test,docker --bam_csi_index + tags: + - bamcsiindex + files: + - path: results/reports/multiqc_report.html + - path: results/preprocessing/GM12878/GM12878.markdup.sorted.bam + - path: results/preprocessing/GM12878/GM12878.markdup.sorted.bam.csi + - path: results/preprocessing/TEST123/TEST123.markdup.sorted.bam + - path: results/preprocessing/TEST123/TEST123.markdup.sorted.bam.csi + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz + - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.csi + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz + - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.csi diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 9e909447..28dd7870 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -48,7 +48,6 @@ ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multi include { INPUT_CHECK } from '../subworkflows/local/input_check' // Validate the input samplesheet.csv and prepare input channels include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' // Build the genome index and other reference files -include { SPLITNCIGAR } from '../subworkflows/local/splitncigar' // Splits reads that contain Ns in their cigar string include { ANNOTATE } from '../subworkflows/local/annotate' // Annotate variants using snpEff or VEP or both /* @@ -79,6 +78,7 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custo include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' // Align reads to genome and sort and index the alignment file include { MARKDUPLICATES } from '../subworkflows/nf-core/markduplicates' // Mark duplicates in the BAM file +include { SPLITNCIGAR } from '../subworkflows/nf-core/splitncigar' // Splits reads that contain Ns in their cigar string include { RECALIBRATE } from '../subworkflows/nf-core/recalibrate' // Estimate and correct systematic bias /* From 31423b347342ce4263cea3d7d090091ffc946b02 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 11:51:57 +0200 Subject: [PATCH 178/235] Changes related to test data file naming and its location --- assets/{samplesheet_full.csv => samplesheet.csv} | 0 assets/samplesheet_test.csv | 2 -- conf/test.config | 2 +- docs/usage.md | 2 +- tests/csv/{samplesheet_test.csv => 1.0/samplesheet.csv} | 0 5 files changed, 2 insertions(+), 4 deletions(-) rename assets/{samplesheet_full.csv => samplesheet.csv} (100%) delete mode 100644 assets/samplesheet_test.csv rename tests/csv/{samplesheet_test.csv => 1.0/samplesheet.csv} (100%) diff --git a/assets/samplesheet_full.csv b/assets/samplesheet.csv similarity index 100% rename from assets/samplesheet_full.csv rename to assets/samplesheet.csv diff --git a/assets/samplesheet_test.csv b/assets/samplesheet_test.csv deleted file mode 100644 index 125f9bfd..00000000 --- a/assets/samplesheet_test.csv +++ /dev/null @@ -1,2 +0,0 @@ -sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse diff --git a/conf/test.config b/conf/test.config index 59c63832..27112de1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = "${baseDir}/tests/csv/samplesheet_test.csv" + input = "${baseDir}/tests/csv/1.0/samplesheet.csv" // Genome references genome = 'WBcel235' diff --git a/docs/usage.md b/docs/usage.md index 6faf9dbe..b2f74e42 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -82,7 +82,7 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,reverse | `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | | `strandedness` | Sample strand-specificity. Must be one of `unstranded`, `forward` or `reverse`. | -An [example samplesheet](../assets/samplesheet_test.csv) has been provided with the pipeline. +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. > **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. diff --git a/tests/csv/samplesheet_test.csv b/tests/csv/1.0/samplesheet.csv similarity index 100% rename from tests/csv/samplesheet_test.csv rename to tests/csv/1.0/samplesheet.csv From 93dd0a79ca46e16795a60d5d71ec3ce74a0271bd Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 12:08:09 +0200 Subject: [PATCH 179/235] Update: CITATIONS.md --- CITATIONS.md | 25 +++++++++++++++++++++++++ 1 file changed, 25 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index 0eae2ebf..a1174039 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,9 +12,34 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- [STAR](https://pubmed.ncbi.nlm.nih.gov/23104886/) + + > Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25. PubMed PMID: 23104886; PubMed Central PMCID: PMC3530905. + +- [SAMtools](https://pubmed.ncbi.nlm.nih.gov/19505943/) + + > Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PubMed PMID: 19505943; PubMed Central PMCID: PMC2723002. + +- [GATK](https://pubmed.ncbi.nlm.nih.gov/20644199/) + + > McKenna A, Hanna M, Banks E, et al.: The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 2010 Sep;20(9):1297-303. doi: 10.1101/gr.107524.110. Epub 2010 Jul 19. PubMed PMID: 20644199; PubMed Central PMCID: PMC2928508. + +- [snpEff](https://pubmed.ncbi.nlm.nih.gov/22728672/) + + > Cingolani P, Platts A, Wang le L, et al.: A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly (Austin). Apr-Jun 2012;6(2):80-92. doi: 10.4161/fly.19695. PubMed PMID: 22728672; PubMed Central PMCID: PMC3679285. + +- [VEP](https://pubmed.ncbi.nlm.nih.gov/27268795/) + + > McLaren W, Gil L, Hunt SE, et al.: The Ensembl Variant Effect Predictor. Genome Biol. 2016 Jun 6;17(1):122. doi: 10.1186/s13059-016-0974-4. PubMed PMID: 27268795; PubMed Central PMCID: PMC4893825. + - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +- [R](https://www.R-project.org/) + + > R Core Team (2017). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) From e39eb6f8d01ee332c253d3a5d0f72f0d3af613d3 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 12:13:00 +0200 Subject: [PATCH 180/235] Update: README.md --- README.md | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 74c68ba5..30d7b35c 100644 --- a/README.md +++ b/README.md @@ -16,9 +16,9 @@ ## Introduction -**nf-core/rnavar** is a bioinformatics best-practice analysis pipeline for GATK4 RNA variant calling pipeline. +**nf-core/rnavar** is a GATK4 best-practice analysis pipeline for RNA variant calling. -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! +The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnavar/results). @@ -47,7 +47,6 @@ On release, automated continuous integration tests run the pipeline on a full-si | FastQC | 0.11.9 | | STAR | 2.7.9a | | Samtools | 1.15 | -| Picard | 2.26.10 | | GATK | 4.2.5.0 | | SnpEff | 5.0 | | Ensembl VEP | 104.3 | From e144189d69e4faad8736e0d0c67dbfed3c2ea451 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 12:17:05 +0200 Subject: [PATCH 181/235] Update: assets/schema_input.json --- assets/schema_input.json | 11 ++++++++--- 1 file changed, 8 insertions(+), 3 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 32bc7f93..f2eb5ea8 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/assets/schema_input.json", - "title": "nf-core/rnavar pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-core/rnaseq/master/assets/schema_input.json", + "title": "nf-core/rnaseq pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { @@ -29,8 +29,13 @@ "maxLength": 0 } ] + }, + "strandedness": { + "type": "string", + "errorMessage": "Strandedness must be provided and be one of 'forward', 'reverse' or 'unstranded'", + "enum": ["forward", "reverse", "unstranded"] } }, - "required": ["sample", "fastq_1"] + "required": ["sample", "fastq_1", "strandedness"] } } From 3541008fc7ffdce2ed94118f1e12fed4c6bd77f4 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 12:18:29 +0200 Subject: [PATCH 182/235] Update: conf/modules.config --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index df225d23..58154736 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -78,7 +78,7 @@ process { ext.args = '--keep-exon-attrs -F -T' publishDir = [ path: { "${params.outdir}/genome" }, - mode: 'copy', + mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ?null : filename }, enabled: params.save_reference ] From e6ea933819a3b072b83a4a7a4cf6e7771f2bc8f9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 12:20:01 +0200 Subject: [PATCH 183/235] Update: removed Path for FASTQC in conf/modules.config --- conf/modules.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 58154736..fc334d0f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -128,7 +128,6 @@ process { withName: FASTQC { ext.args = '--quiet' publishDir = [ - path: { "${params.outdir}/reports/fastqc/${meta.id}" }, enabled: false ] } From 882e465f67b8b0226ae648068e5c60245ea61e58 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 13:11:28 +0200 Subject: [PATCH 184/235] Updated: README.md --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 30d7b35c..afd8179e 100644 --- a/README.md +++ b/README.md @@ -16,9 +16,9 @@ ## Introduction -**nf-core/rnavar** is a GATK4 best-practice analysis pipeline for RNA variant calling. +**nf-core/rnavar** is a bioinformatics best-practice analysis pipeline for GATK4 RNA variant calling. -The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/rnavar/results). From 9f5a98b99d63c3e3f97db11fc3661ba11f9cd7b1 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 13:44:19 +0200 Subject: [PATCH 185/235] Update: usage.md --- docs/usage.md | 55 +++++++++++++-------------------------------------- 1 file changed, 14 insertions(+), 41 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index b2f74e42..87d0b035 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -126,7 +126,7 @@ STAR alignment generates a coordinated-sorted BAM file as output. The coordinate ## Preprocessing options -Marking duplicate reads is performed using `Picard MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. +Marking duplicate reads is performed using `GATK MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. GATK best practices has been followed in this pipeline for RNA analysis, hence it uses GATK modules such as `SplitNCigarReads`, `BaseRecalibrator`, `ApplyBQSR`. The `BaseRecalibrator` process requires known variants sites VCF. ExAc, gnomAD, or dbSNP resources can be used as known sites of variation.You can supply the VCF and index files using parameters such as `--dbsnp`, `--dbsnp_tbi`, `--known_indels`, `--known_indels_tbi`. @@ -249,11 +249,11 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. -## CUSTOME CONFIGURATIONS +## CUSTOM CONFIGURATIONS ### Resource requests -Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnavar/blob/master/conf/base.config#L17) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. For example, if the nf-core/rnavar pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: @@ -286,7 +286,14 @@ Work dir: Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` ``` -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. +The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnavar Github repo](https://github.com/nf-core/rnavar/search?q=process+STAR_ALIGN). +We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. +If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnavar/blob/master/modules/nf-core/modules/star/align/main.nf#L3). +The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. +The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnavar/blob/master/conf/base.config#L37-L41) which in this case is defined as `72GB`. +Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least `72GB` of memory, in this case increased to `100GB`. +The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ```nextflow process { @@ -296,43 +303,9 @@ process { } ``` -> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNAVAR:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. - -### Tool-specific options - -For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes. - -The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: - -- Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. - -- An [`options.args`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. - -The nf-core/rnavar pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. - -When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnavar.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnavar.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. - -As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. - -Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnavar/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. - -As you will see in the example below, we have: - -- appended `--outFilterMismatchNmax 16` to the default `args` used by the module. -- changed the default `publish_dir` value to where the files will eventually be published in the main results directory. -- appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`. - -```nextflow -params { - modules { - 'star_align' { - args = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16" - publish_dir = "my_star_directory" - publish_files = ['out':'log', 'tab':'log', 'bam':''] - } - } -} -``` +> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. +> +> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. ### Updating containers From fc7a58e07ccb3dc171131b93e5106a01c9681f4e Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 13:48:42 +0200 Subject: [PATCH 186/235] Update: review feedback corrections --- README.md | 2 +- docs/usage.md | 2 +- workflows/rnavar.nf | 4 ++-- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index afd8179e..de25dd5a 100644 --- a/README.md +++ b/README.md @@ -28,7 +28,7 @@ On release, automated continuous integration tests run the pipeline on a full-si 2. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 3. Align reads to reference genome ([`STAR`](https://github.com/alexdobin/STAR)) 4. Sort and index alignments ([`SAMtools`](https://sourceforge.net/projects/samtools/files/samtools/)) -5. Duplicate read marking ([`Picard MarkDuplicates`](https://broadinstitute.github.io/picard/)) +5. Duplicate read marking ([`GATK4 MarkDuplicates`](https://gatk.broadinstitute.org/hc/en-us/articles/360037052812-MarkDuplicates-Picard)) 6. Splits reads that contain Ns in their cigar string ([`GATK4 SplitNCigarReads`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917482651-SplitNCigarReads)) 7. Estimate and correct systematic bias using base quality score recalibration ([`GATK4 BaseRecalibrator`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897206043-BaseRecalibrator), [`GATK4 ApplyBQSR`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897168667-ApplyBQSR)) 8. Convert a BED file to a Picard Interval List ([`GATK4 BedToIntervalList`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924780827-BedToIntervalList-Picard-)) diff --git a/docs/usage.md b/docs/usage.md index 87d0b035..f2692bb2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -126,7 +126,7 @@ STAR alignment generates a coordinated-sorted BAM file as output. The coordinate ## Preprocessing options -Marking duplicate reads is performed using `GATK MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. +Marking duplicate reads is performed using `GATK4 MarkDuplicates` tool. The tool does not remove duplicate reads by default, however you can set `--remove_duplicates true` to remove them. GATK best practices has been followed in this pipeline for RNA analysis, hence it uses GATK modules such as `SplitNCigarReads`, `BaseRecalibrator`, `ApplyBQSR`. The `BaseRecalibrator` process requires known variants sites VCF. ExAc, gnomAD, or dbSNP resources can be used as known sites of variation.You can supply the VCF and index files using parameters such as `--dbsnp`, `--dbsnp_tbi`, `--known_indels`, `--known_indels_tbi`. diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 28dd7870..02a3b29e 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -29,7 +29,7 @@ for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } -if(!params.star_index && !params.gtf && !params.gff){ exit 1, "GTF|GFF3 file is required to build a STAR reference index! Use option -gtf|-gff to provide a GTF|GFF file." } +if(!params.star_index && !params.gtf && !params.gff){ exit 1, "GTF|GFF3 file is required to build a STAR reference index! Use option --gtf|--gff to provide a GTF|GFF file." } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -219,7 +219,7 @@ workflow RNAVAR { ch_star_multiqc = ALIGN_STAR.out.log_final ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) - // SUBWORKFLOW: Mark duplicates with Picard + // SUBWORKFLOW: Mark duplicates with GATK4 MARKDUPLICATES(ch_genome_bam) ch_genome_bam = MARKDUPLICATES.out.bam_bai ch_samtools_stats = MARKDUPLICATES.out.stats From 317498b00cb4de1a9d4cbdccb2634de8d42fe45a Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 13:50:26 +0200 Subject: [PATCH 187/235] Correction: ext.args of HC --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index fc334d0f..861be763 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -301,7 +301,7 @@ process { withName: GATK4_HAPLOTYPECALLER { ext.args = [ - '-dont-use-soft-clipped-bases', + '--dont-use-soft-clipped-bases', params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '', params.bam_csi_index ? "--create-output-variant-index false" : "" ].join(' ').trim() From 73facdc1e9991cecf78fc4f7fd980c26761e1693 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 4 May 2022 13:56:55 +0200 Subject: [PATCH 188/235] update: assets/schema_input.json --- assets/schema_input.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index f2eb5ea8..6fd501da 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/rnaseq/master/assets/schema_input.json", - "title": "nf-core/rnaseq pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-core/rnavar/master/assets/schema_input.json", + "title": "nf-core/rnavar pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { From aa72ab3aa2878e572a4ed04aae206c9f896a98b0 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 5 May 2022 09:23:03 +0200 Subject: [PATCH 189/235] Edited: test_full.config --- conf/test_full.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/test_full.config b/conf/test_full.config index 38e65a51..a60cc21d 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,7 +15,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Parameters for full-size test - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.1/samplesheet_full.csv' - genome = 'GRCh37' - pseudo_aligner = 'salmon' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.4/samplesheet_full.csv' + read_length = 101 + genome = 'GRCh38' } From 5abe7d2d661b365d75d3f2ff33e203efce64f687 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 5 May 2022 09:26:42 +0200 Subject: [PATCH 190/235] Added: default genome as GRCh38 --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index e6459470..30b940a1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -15,7 +15,7 @@ params { input = null // sample sheet // Genome and reference options - genome = null + genome = 'GRCh38' igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_ignore = false save_reference = false From 471abee2d1fcd858dc988b4c2ced7f3ace148d2f Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Thu, 5 May 2022 11:52:04 +0200 Subject: [PATCH 191/235] Updated the samplesheet link --- conf/test_full.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_full.config b/conf/test_full.config index a60cc21d..81133bdc 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,7 +15,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Parameters for full-size test - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.4/samplesheet_full.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnavar/samplesheet/v1.0/samplesheet_full.csv' read_length = 101 genome = 'GRCh38' } From 85fc2379d10837704b484159c166198002ba15f9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 12:21:14 +0200 Subject: [PATCH 192/235] Changed munin profile to test in OUTPUT.md --- docs/output.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index 35c2915c..21001305 100644 --- a/docs/output.md +++ b/docs/output.md @@ -24,10 +24,10 @@ GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz, The pipeline has been executed with the following command. ```console -nextflow run nf-core/rnavar -profile munin,docker --input samplesheet.csv --genome GRCh38 --read_length 151 --annotate_tools merge +nextflow run nf-core/rnavar -profile test,docker --input samplesheet.csv --genome GRCh38 --read_length 151 --annotate_tools merge ``` -It used an institutional profile `munin` from [nf-core/configs](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config) +It used the test profile provided with this pipeline which can be viewed [here](https://raw.githubusercontent.com/nf-core/rnavar/master/conf/test.config) ## Pipeline overview From 591657fd2d071c732a3fd887ddb7b773a4a541c4 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 12:27:42 +0200 Subject: [PATCH 193/235] Updated OUTPUT.md --- docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index 21001305..6acbca92 100644 --- a/docs/output.md +++ b/docs/output.md @@ -28,7 +28,7 @@ nextflow run nf-core/rnavar -profile test,docker --input samplesheet.csv --genom ``` It used the test profile provided with this pipeline which can be viewed [here](https://raw.githubusercontent.com/nf-core/rnavar/master/conf/test.config) - +However, you can create your own institutional profile and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the profile name directly here in the command instead of `test` to best use your data and parameters. ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: From 30895dbb7bd54dbff186deeda87d5d1d13d1fd45 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 12:36:21 +0200 Subject: [PATCH 194/235] Deleted .prettierignore --- .prettierignore | 9 --------- docs/output.md | 2 +- modules.json | 2 +- 3 files changed, 2 insertions(+), 11 deletions(-) delete mode 100644 .prettierignore diff --git a/.prettierignore b/.prettierignore deleted file mode 100644 index d0e7ae58..00000000 --- a/.prettierignore +++ /dev/null @@ -1,9 +0,0 @@ -email_template.html -.nextflow* -work/ -data/ -results/ -.DS_Store -testing/ -testing* -*.pyc diff --git a/docs/output.md b/docs/output.md index 6acbca92..e504b1e6 100644 --- a/docs/output.md +++ b/docs/output.md @@ -24,7 +24,7 @@ GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz, The pipeline has been executed with the following command. ```console -nextflow run nf-core/rnavar -profile test,docker --input samplesheet.csv --genome GRCh38 --read_length 151 --annotate_tools merge +nextflow run nf-core/rnavar -profile test,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge ``` It used the test profile provided with this pipeline which can be viewed [here](https://raw.githubusercontent.com/nf-core/rnavar/master/conf/test.config) diff --git a/modules.json b/modules.json index 3f7997cc..61e0b776 100644 --- a/modules.json +++ b/modules.json @@ -98,4 +98,4 @@ } } } -} +} \ No newline at end of file From c5501accc430213d05c5029907e30a75ca32b15e Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 12:43:47 +0200 Subject: [PATCH 195/235] Added: citation for Tabix --- CITATIONS.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index a1174039..d84e03ce 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -36,6 +36,10 @@ > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +- [Tabix]() + + > Heng Li, Tabix: fast retrieval of sequence features from generic TAB-delimited files, Bioinformatics, Volume 27, Issue 5, 1 March 2011, Pages 718–719. doi: 10.1093/bioinformatics/btq671. PubMed PMID: 21208982; PubMed Central PMCID: PMC3042176. + - [R](https://www.R-project.org/) > R Core Team (2017). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. From b6ac130a209edb2ecd4e5c30364857938df01e2f Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 12:48:16 +0200 Subject: [PATCH 196/235] Updated samtools version in README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index de25dd5a..f1baf004 100644 --- a/README.md +++ b/README.md @@ -46,7 +46,7 @@ On release, automated continuous integration tests run the pipeline on a full-si | ----------- | ------- | | FastQC | 0.11.9 | | STAR | 2.7.9a | -| Samtools | 1.15 | +| Samtools | 1.15.1 | | GATK | 4.2.5.0 | | SnpEff | 5.0 | | Ensembl VEP | 104.3 | From 4676cece9f7d65c665bd7396208d79aa39c78014 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 13:38:26 +0200 Subject: [PATCH 197/235] Update link in check_samplesheet.py --- bin/check_samplesheet.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index d1a9919e..5362126c 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -169,15 +169,15 @@ def check_samplesheet(file_in, file_out): Example: This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: + see also the `rnavar test samplesheet`_:: sample,fastq_1,fastq_2 SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + .. _rnavar test samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/rnavar/samplesheet/v1.0/samplesheet.csv """ required_columns = {"sample", "fastq_1", "fastq_2"} From 546015122d497ef0badd523d7c83656adccfa67e Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 13:39:48 +0200 Subject: [PATCH 198/235] Removed commented line in conf/test.config --- conf/test.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 27112de1..e931a9a8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,7 +25,6 @@ params { // Genome references genome = 'WBcel235' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' - //fasta_fai = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta.fai' dict = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.dict' gtf = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.gtf' From e78e5b51253b6ba5f0087f94c5b76605f8a7995c Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 14:44:31 +0200 Subject: [PATCH 199/235] Review corrections --- conf/modules.config | 2 +- docs/usage.md | 6 ++---- subworkflows/nf-core/align_star.nf | 10 ++-------- subworkflows/nf-core/bam_sort_samtools.nf | 10 +++------- subworkflows/nf-core/bam_stats_samtools.nf | 8 +++----- subworkflows/nf-core/ensemblvep_annotate.nf | 14 +------------- subworkflows/nf-core/markduplicates.nf | 10 +++------- subworkflows/nf-core/recalibrate.nf | 11 +++-------- subworkflows/nf-core/snpeff_annotate.nf | 13 +------------ subworkflows/nf-core/splitncigar.nf | 9 +++++---- 10 files changed, 24 insertions(+), 69 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 861be763..f3534223 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -374,7 +374,7 @@ process { } } -if( (params.annotate_tools) && (params.annotate_tools.contains('snpeff') || params.annotate_tools.contains('merge')) ) { +if( (params.annotate_tools) && (params.annotate_tools.contains('snpeff') ) { process { withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:SNPEFF_ANNOTATE:TABIX_BGZIPTABIX' { ext.prefix = {"${meta.id}_snpEff.ann.vcf"} diff --git a/docs/usage.md b/docs/usage.md index f2692bb2..3f66258b 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -18,7 +18,7 @@ Note that the pipeline will create the following files in your working directory ```console work # Directory containing the nextflow working files - # Finished results in specified location (defined with --outdir) + # Finished results in specified location (defined with --outdir) .nextflow_log # Log file from Nextflow # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` @@ -84,8 +84,6 @@ TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz,,reverse An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. -> **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v3.1 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns, however it now also offers more flexibility in instances where replicate information is not required e.g. when sequencing clinical samples. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. - ## PIPELINE PARAMETERS AND DESCRIPTION ## Reference genome files @@ -169,7 +167,7 @@ nextflow run nf-core/rnavar --input samplesheet.csv --genome GRCh38 -profile doc ### Download annotation cache -A `Nextflow` helper script has been designed to help downloading `snpEff` and `VEP` caches. +A `Nextflow` helper script [link](https://raw.githubusercontent.com/nf-core/sarek/master/download_cache.nf) has been designed to help downloading `snpEff` and `VEP` caches. Such files are meant to be shared between multiple users, so this script is mainly meant for people administrating servers, clusters and advanced users. ```bash diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index 1a8d7bab..87e482db 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -2,14 +2,8 @@ // Alignment with STAR // -//params.align_options = [:] -//params.samtools_index_options = [:] -//params.samtools_sort_options = [:] -//params.samtools_stats_options = [:] -//params.seq_platform = [:] - -include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' //addParams( options: params.align_options, seq_platform: params.seq_platform) -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' //addParams( sort_options: params.samtools_sort_options, index_options: params.samtools_index_options, stats_options: params.samtools_stats_options ) +include { STAR_ALIGN } from '../../modules/nf-core/modules/star/align/main' +include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' workflow ALIGN_STAR { take: diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index 1746c834..d735bd37 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -2,13 +2,9 @@ // Sort, index BAM file and run samtools stats, flagstat and idxstats // -//params.sort_options = [:] -//params.index_options = [:] -//params.stats_options = [:] - -include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' //addParams( options: params.sort_options ) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams( options: params.index_options ) -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' //addParams( options: params.stats_options ) +include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' workflow BAM_SORT_SAMTOOLS { take: diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index 34ab083c..3d505a4d 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -2,11 +2,9 @@ // Run SAMtools stats, flagstat and idxstats // -//params.options = [:] - -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' //addParams( options: params.options ) -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' //addParams( options: params.options ) -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' //addParams( options: params.options ) +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' workflow BAM_STATS_SAMTOOLS { take: diff --git a/subworkflows/nf-core/ensemblvep_annotate.nf b/subworkflows/nf-core/ensemblvep_annotate.nf index 4be2cbb5..40572a8b 100644 --- a/subworkflows/nf-core/ensemblvep_annotate.nf +++ b/subworkflows/nf-core/ensemblvep_annotate.nf @@ -2,19 +2,8 @@ // Run VEP to annotate VCF files // -//params.bgziptabix_vep = [:] -//params.vep_options = [:] -//params.vep_tag = [:] -//params.use_cache = [:] - include { ENSEMBLVEP } from '../../modules/nf-core/modules/ensemblvep/main' -//addParams( -// options: params.vep_options, -// use_cache: params.use_cache, -// vep_tag: params.vep_tag -//) - -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' //addParams(options: params.bgziptabix_vep_options) +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' workflow ENSEMBLVEP_ANNOTATE { take: @@ -23,7 +12,6 @@ workflow ENSEMBLVEP_ANNOTATE { vep_species // value: which species vep_cache_version // value: which cache version vep_cache // path: path_to_vep_cache (optionnal) - // skip // boolean: true/false main: diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index d9b4df2b..b1c41edc 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -2,13 +2,9 @@ // GATK4 MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats // -//params.markduplicates_options = [:] -//params.samtools_index_options = [:] -//params.samtools_stats_options = [:] - -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' //addParams(options: params.samtools_stats_options) -include { GATK4_MARKDUPLICATES } from '../../modules/nf-core/modules/gatk4/markduplicates/main' //addParams(options: params.markduplicates_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) +include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' +include { GATK4_MARKDUPLICATES } from '../../modules/nf-core/modules/gatk4/markduplicates/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' workflow MARKDUPLICATES { take: diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 17e1e977..010f8be1 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -4,14 +4,9 @@ ======================================================================================== */ -//params.applybqsr_options = [:] -//params.qualimap_bamqc_options = [:] -//params.samtools_index_options = [:] -//params.samtools_stats_options = [:] - -include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' //addParams(options: params.applybqsr_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' //addParams(options: params.samtools_stats_options) +include { GATK4_APPLYBQSR as APPLYBQSR } from '../../modules/nf-core/modules/gatk4/applybqsr/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' workflow RECALIBRATE { take: diff --git a/subworkflows/nf-core/snpeff_annotate.nf b/subworkflows/nf-core/snpeff_annotate.nf index e9183e47..b7b618d0 100644 --- a/subworkflows/nf-core/snpeff_annotate.nf +++ b/subworkflows/nf-core/snpeff_annotate.nf @@ -2,19 +2,8 @@ // Run snpEff to annotate VCF files // -//params.bgziptabix_snpeff = [:] -//params.snpeff_options = [:] -//params.snpeff_tag = [:] -//params.use_cache = [:] - include { SNPEFF } from '../../modules/nf-core/modules/snpeff/main' -//addParams( -// options: params.snpeff_options, -// snpeff_tag: params.snpeff_tag, -// use_cache: params.use_cache -//) - -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' //addParams(options: params.bgziptabix_snpeff_options) +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/modules/tabix/bgziptabix/main' workflow SNPEFF_ANNOTATE { take: diff --git a/subworkflows/nf-core/splitncigar.nf b/subworkflows/nf-core/splitncigar.nf index 6f941a35..150c5bc1 100644 --- a/subworkflows/nf-core/splitncigar.nf +++ b/subworkflows/nf-core/splitncigar.nf @@ -3,8 +3,8 @@ // include { GATK4_SPLITNCIGARREADS } from '../../modules/nf-core/modules/gatk4/splitncigarreads/main' -include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' //addParams(options: params.samtools_merge_options) -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' //addParams(options: params.samtools_index_options) +include { SAMTOOLS_MERGE } from '../../modules/nf-core/modules/samtools/merge/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' workflow SPLITNCIGAR { take: @@ -32,8 +32,9 @@ workflow SPLITNCIGAR { bam_splitncigar .map{ meta, bam -> - meta.id = meta.sample - [meta, bam] + new_meta = meta.clone() + new_meta.id = meta.sample + [new_meta, bam] }.groupTuple().set{bam_splitncigar_interval} SAMTOOLS_MERGE(bam_splitncigar_interval, fasta) From 73f7dfb5c5675f95e0d69c3e1d29be13401a554a Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 9 May 2022 14:45:33 +0200 Subject: [PATCH 200/235] Fix syntax error --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index f3534223..0c0d774b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -374,7 +374,7 @@ process { } } -if( (params.annotate_tools) && (params.annotate_tools.contains('snpeff') ) { +if( (params.annotate_tools) && (params.annotate_tools.contains('snpeff')) ) { process { withName: 'NFCORE_RNAVAR:RNAVAR:ANNOTATE:SNPEFF_ANNOTATE:TABIX_BGZIPTABIX' { ext.prefix = {"${meta.id}_snpEff.ann.vcf"} From 1f8354ab44be9843bae257e1bf1d01c0c4b80a97 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 11 May 2022 15:25:55 +0200 Subject: [PATCH 201/235] Changed: command used for this experiment --- docs/output.md | 11 +++++------ 1 file changed, 5 insertions(+), 6 deletions(-) diff --git a/docs/output.md b/docs/output.md index e504b1e6..4b94dc56 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,7 +2,7 @@ ## Introduction -This document describes the output produced by the pipeline based on public data. +This document describes the output produced by the pipeline based on a public dataset. The directories listed below are created in the `results/` directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -24,18 +24,16 @@ GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz, The pipeline has been executed with the following command. ```console -nextflow run nf-core/rnavar -profile test,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge +nextflow run nf-core/rnavar -profile ,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge ``` -It used the test profile provided with this pipeline which can be viewed [here](https://raw.githubusercontent.com/nf-core/rnavar/master/conf/test.config) -However, you can create your own institutional profile and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the profile name directly here in the command instead of `test` to best use your data and parameters. +The `` used in this experiment can be found [here](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config). However, you can create your own institutional profile and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the profile name directly in the command instead of `` to use your own data and parameters. ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - [Preprocessing](#preprocessing) - [cat](#cat) - Merge re-sequenced FastQ files - - [FastQC](#fastqc) - Raw read QC - [Alignment](#alignment) - [STAR](#star) - Fast spliced aware genome alignment - [Alignment post-processing](#alignment-post-processing) @@ -47,7 +45,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Variant filtering](#other-steps) - [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria - [Quality control](#quality-control) - - [MultiQC](#multiqc) - Present QC for raw reads, alignment, read counting and sample similiarity + - [FastQC](#fastqc) - Raw read QC + - [MultiQC](#multiqc) - Present QC for raw reads from FASTQC - [Workflow reporting and genomes](#workflow-reporting-and-genomes) - [Reference genome files](#reference-genome-files) - Saving reference genome indices/files - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution From 29d400b8ac6097a9be3d38e988ef24cabdf544d6 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 11 May 2022 15:37:00 +0200 Subject: [PATCH 202/235] Edited output.md --- docs/output.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/docs/output.md b/docs/output.md index 4b94dc56..c5d92bbb 100644 --- a/docs/output.md +++ b/docs/output.md @@ -24,10 +24,10 @@ GM12878,/data/GM12878/SRR5665260_1.fastq.gz,/data/GM12878/SRR5665260_2.fastq.gz, The pipeline has been executed with the following command. ```console -nextflow run nf-core/rnavar -profile ,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge +nextflow run nf-core/rnavar -profile ,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge --outdir results ``` -The `` used in this experiment can be found [here](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config). However, you can create your own institutional profile and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the profile name directly in the command instead of `` to use your own data and parameters. +The `` used in this experiment can be found [here](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config). However, you can create your own institutional config and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the config name directly in the command instead of `` to use your own data and parameters. ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: From 906ba5568b7b0775684ef5a37f1c072bf85543a0 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 11 May 2022 15:48:30 +0200 Subject: [PATCH 203/235] Fix: python version in samplesheet_check.nf --- modules/local/samplesheet_check.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 76014d0d..7667d217 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -1,10 +1,10 @@ process SAMPLESHEET_CHECK { tag "$samplesheet" - conda (params.enable_conda ? "conda-forge::python=3.9.5" : null) + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.9--1' : - 'quay.io/biocontainers/python:3.9--1' }" + 'https://depot.galaxyproject.org/singularity/python:3.8.3' : + 'quay.io/biocontainers/python:3.8.3' }" input: path samplesheet From 8564ef87ed59cb9b626a8b069671db18ea1ad1a4 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 12 May 2022 16:17:54 +0200 Subject: [PATCH 204/235] Add: multiQC integration; Update doc with more content and plots; --skip_qc added, publish additional stats files --- conf/modules.config | 40 +++-- docs/images/alignment_star_mapping_stats.png | Bin 0 -> 44374 bytes docs/images/fastqc_gc_content.png | Bin 0 -> 40449 bytes docs/images/fastqc_length_dist.png | Bin 0 -> 28396 bytes docs/images/fastqc_mean_qual_score_dist.png | Bin 0 -> 33085 bytes docs/images/fastqc_overall_status.png | Bin 0 -> 25396 bytes docs/images/fastqc_seq_counts.png | Bin 0 -> 31505 bytes docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/images/multiqc_report.png | Bin 0 -> 111664 bytes docs/images/read_markduplicates_stats.png | Bin 0 -> 42491 bytes docs/images/snpeff_variant_quality.png | Bin 0 -> 19419 bytes .../snpeff_variants_by_effect_types.png | Bin 0 -> 64345 bytes docs/images/snpeff_variants_by_impact.png | Bin 0 -> 26424 bytes docs/images/snpeff_variants_by_region.png | Bin 0 -> 55399 bytes docs/images/vep_general_stats.png | Bin 0 -> 50973 bytes docs/images/vep_pph_summary.png | Bin 0 -> 26823 bytes docs/images/vep_sift_summary.png | Bin 0 -> 24816 bytes docs/output.md | 153 ++++++++++++++++-- subworkflows/nf-core/recalibrate.nf | 1 - tests/test_annotation.yml | 8 +- workflows/rnavar.nf | 71 ++++---- 23 files changed, 214 insertions(+), 59 deletions(-) create mode 100644 docs/images/alignment_star_mapping_stats.png create mode 100644 docs/images/fastqc_gc_content.png create mode 100644 docs/images/fastqc_length_dist.png create mode 100644 docs/images/fastqc_mean_qual_score_dist.png create mode 100644 docs/images/fastqc_overall_status.png create mode 100644 docs/images/fastqc_seq_counts.png delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 docs/images/multiqc_report.png create mode 100644 docs/images/read_markduplicates_stats.png create mode 100644 docs/images/snpeff_variant_quality.png create mode 100644 docs/images/snpeff_variants_by_effect_types.png create mode 100644 docs/images/snpeff_variants_by_impact.png create mode 100644 docs/images/snpeff_variants_by_region.png create mode 100644 docs/images/vep_general_stats.png create mode 100644 docs/images/vep_pph_summary.png create mode 100644 docs/images/vep_sift_summary.png diff --git a/conf/modules.config b/conf/modules.config index 0c0d774b..721b4c05 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -121,7 +121,8 @@ process { publishDir = [ path: { "${params.outdir}/reports"}, mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: !params.skip_qc ] } @@ -151,7 +152,7 @@ process { ].join(' ').trim() publishDir = [ [ - path: { "${params.outdir}/preprocessing/${meta.id}/log" }, + path: { "${params.outdir}/reports/stats/${meta.id}/STAR_log" }, mode: params.publish_dir_mode, pattern: '*.{out,tab}' ], @@ -218,10 +219,18 @@ process { ].join(' ').trim() ext.prefix = {"${meta.id}.markdup.sorted"} publishDir = [ - path: { "${params.outdir}/preprocessing/${meta.id}" }, - mode: params.publish_dir_mode, - enabled: true, - pattern: "*.{bam}" + [ + path: { "${params.outdir}/preprocessing/${meta.id}" }, + mode: params.publish_dir_mode, + enabled: true, + pattern: "*.{bam}" + ], + [ + path: { "${params.outdir}/reports/stats/${meta.id}" }, + mode: params.publish_dir_mode, + enabled: true, + pattern: "*.{metrics}" + ] ] } @@ -250,9 +259,9 @@ process { withName: 'SAMTOOLS_STATS|SAMTOOLS_FLAGSTAT|SAMTOOLS_IDXSTATS' { publishDir = [ - path: { "${params.outdir}/reports/samtools_stats/${meta.id}" }, + path: { "${params.outdir}/reports/stats/${meta.id}" }, mode: params.publish_dir_mode, - enabled: true, + enabled: !params.skip_qc, pattern: "*.{stats,flagstat}" ] } @@ -353,7 +362,12 @@ process { if(!params.vep_cache){ container = { params.genome ? "nfcore/vep:104.3.${params.genome}" : "nfcore/vep:104.3.${params.vep_genome}" } } - publishDir = [ enabled: false ] + publishDir = [ + mode: params.publish_dir_mode, + path: { "${params.outdir}/reports/EnsemblVEP/${meta.id}" }, + pattern: "*html", + enabled: !params.skip_qc + ] } withName: 'SNPEFF' { @@ -361,7 +375,13 @@ process { if(!params.snpeff_cache){ container = { params.genome ? "nfcore/snpeff:5.0.${params.genome}" : "nfcore/snpeff:5.0.${params.snpeff_db.split('\\.')[0]}" } } - publishDir = [ enabled: false ] + publishDir = [ + mode: params.publish_dir_mode, + path: { "${params.outdir}/reports/SnpEff/${meta.id}" }, + pattern: "*.{csv,html,txt}", + saveAs: { params.annotate_tools.contains('snpeff') ? it : null }, + enabled: !params.skip_qc + ] } withName: 'TABIX_BGZIPTABIX' { diff --git a/docs/images/alignment_star_mapping_stats.png b/docs/images/alignment_star_mapping_stats.png new file mode 100644 index 0000000000000000000000000000000000000000..d6a27911d6736dba2a17f130e478546a0eb05b22 GIT binary patch literal 44374 zcmeEu2UJtr)2~-WMVf&0rc@10K|ne}0HqoTy-Amj^cD~mr3gruE}@0qi4cmQbO9-$ zh2Bd-CrAmrc$NGA-uqv__10V8x87Up-MtQr!wGxO?Afz_Gka!ELLRFrl3b;~dhXmg zl1C4L8t2Yk5RAn-bvv zCUkzN=X&lO>E|Cmf3!KlEY6)PTzdq30QNG*;2_S|b<&oIjv@~-+0w$_MIZ+#V<-{H;E}%wHQbC@x5?xn7WZprUE8 z@$Cx-km`KA3cMm!Jh#XA+hA=&u61N7!r=fq>M4G+KeYmNOxHj1+?LV}VqS#&o=1(l 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z4L?E+HXW#O?z`rEhjW5-w8GWk%MPAWj0mn=DVnV4;$O zFfyirn~B2(1O*OxxHVMj@j)GfZnBYm`bsvfktHRK4OJ1<^%s7-d#hl4aO4KKuF~lZ zxIQTEU5=!FUr;sYA)a#@yrDo1T#m9!+fM#nDgjlKlc(ZmJJi{(s_0w& zr@V}U<8JIBH}03YrP}*Tj2Fo{&4ku@^4=aV8upCxU>m#z@j$e9C%I6+lr%$XU1NY? z3`u`*^qPJ#Gha@HGZItV;KKl7{zTLaSW+p)Dwu*Ckm)OO!p%uy`VN zcuVPHmOJiwxIz1BN<#5JugL0opNE1343MBdwj+)?DICKLaTh=< zDc;J0=^_1iVIRQ)PqmwU{q5`Jq~5m7xbu2rv*@>LKB}2%MiAdjO#rIfkOC?(`shH^ z7-_8G-*m}Y?j$~^`cC$46KmV5xg^@W{r|88&~EyYn,docker --input samplesheet.csv --genome GRCh38 --annotate_tools merge --outdir results ``` The `` used in this experiment can be found [here](https://github.com/nf-core/configs/blob/master/conf/pipeline/rnavar/munin.config). However, you can create your own institutional config and place it on [nf-core/configs](https://github.com/nf-core/configs/tree/master/conf/pipeline/rnavar) and then use the config name directly in the command instead of `` to use your own data and parameters. + ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: @@ -44,9 +45,18 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [GATK4 HaplotypeCaller](#gatk4-haplotypecaller) - Call SNPs and indels via local re-assembly of haplotypes - [Variant filtering](#other-steps) - [GATK4 VariantFiltration](#gatk4-variantfiltration) - Hard-filtering variant calls based on certain criteria -- [Quality control](#quality-control) - - [FastQC](#fastqc) - Raw read QC - - [MultiQC](#multiqc) - Present QC for raw reads from FASTQC +- [Variant annotation](#variant-annotation) + - [snpEff](#snpeff) + - [VEP](#vep) +- [QC and reporting](#qc-and-reporting) + - [QC](#qc) + - [FastQC](#fastqc) - Raw read QC + - [GATK MarkDuplicates reports](#gatk-markduplicates-reports) + - [samtools stats](#samtools-stats) + - [snpEff reports](#snpeff-reports) + - [VEP reports](#vep-reports) + - [Reporting](#reporting) + - [MultiQC](#multiqc) - Present QC for raw reads, alignment, base quality recalibration as well as variant annotation summary. - [Workflow reporting and genomes](#workflow-reporting-and-genomes) - [Reference genome files](#reference-genome-files) - Saving reference genome indices/files - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution @@ -71,6 +81,8 @@ If multiple libraries/runs have been provided for the same sample in the input s [STAR](https://github.com/alexdobin/STAR) is a read aligner designed for splice aware mapping typical of RNA sequencing data. STAR stands for *S*pliced *T*ranscripts *A*lignment to a *R*eference, and has been shown to have high accuracy and outperforms other aligners by more than a factor of 50 in mapping speed, but it is memory intensive. +![MultiQC - STAR Alignment stats](images/alignment_star_mapping_stats.png) +
    Output files @@ -83,7 +95,7 @@ If multiple libraries/runs have been provided for the same sample in the input s - `[SAMPLE].SJ.out.tab`: File containing filtered splice junctions detected after mapping the reads. - `preprocessing/[SAMPLE]/unmapped` - `[SAMPLE].unmapped_*.fastq.gz`: If `--save_unaligned` is specified, FastQ files containing unmapped reads will be placed in this directory. -- `reports/samtools_stats/[SAMPLE]/` +- `reports/stats/[SAMPLE]/` - `[SAMPLE].aligned.bam.flagstat`: Samtools flagstat summary of the alignment - `[SAMPLE].aligned.bam.stats`: Samtools stat output @@ -95,6 +107,8 @@ If multiple libraries/runs have been provided for the same sample in the input s [GATK MarkDuplicates](https://gatk.broadinstitute.org/hc/en-us/articles/360042477492-MarkDuplicates-Picard) locates and tags duplicate reads in a `BAM` file. The tool's main output is a new BAM file, in which duplicates have been identified in the SAM flags field for each read. +![MultiQC - MarkDuplicate stats](images/read_markduplicates_stats.png) + If desired, duplicates can be removed using the `--remove_duplicates true` option.
    @@ -103,9 +117,6 @@ If desired, duplicates can be removed using the `--remove_duplicates true` optio - `preprocessing/[SAMPLE]/` - `[SAMPLE].markdup.sorted.bam`: Picard Markduplicate bam file. - `[SAMPLE].markdup.sorted.bam.bai`: This is the index of the above \*.aligned.bam -- `reports/samtools_stats/[SAMPLE]/` - - `[SAMPLE].markdup.sorted.bam.flagstat`: Samtools flagstat summary of the alignment - - `[SAMPLE].markdup.sorted.bam.stats`: Samtools stat output
    @@ -177,6 +188,11 @@ The generated `VCF` header contains the software version and the used command li To annotate variants using `snpeff`, you can use `--annotate_tools snpeff` or `--annotate_tools merge`. The annotated variant files in VCF format can be found in `results/variant_annotation` folder. +![MultiQC - snpEff variant by region](images/snpeff_variants_by_region.png) +![MultiQC - snpEff variant by impact](images/snpeff_variants_by_impact.png) +![MultiQC - snpEff variant by effect types](images/snpeff_variants_by_effect_types.png) +![MultiQC - snpEff variant by quality](images/snpeff_variant_quality.png) +
    Output files @@ -210,6 +226,10 @@ Currently, it contains: To annotate variants using `vep`, you can use `--annotate_tools vep`. The annotated variant files in VCF format can be found in `results/variant_annotation` folder. +![MultiQC - VEP general statistics](images/vep_general_stats.png) +![MultiQC - VEP SIFT summary](images/vep_sift_summary.png) +![MultiQC - VEP Polyphen summary](images/vep_pph_summary.png) +
    Output files @@ -232,20 +252,127 @@ When `--annotate_tools merge` option is used, the annotation from both `snpeff` For further reading and documentation see the [VEP manual](https://www.ensembl.org/info/docs/tools/vep/index.html) -### MultiQC report with FASTQC read summary +## QC and Reporting + +### QC + +#### FastQC + +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +Plots will show: + +- Sequence counts for each sample. +- Sequence Quality Histograms: The mean quality value across each base position in the read. +- Per Sequence Quality Scores: The number of reads with average quality scores. Shows if a subset of reads has poor quality. +- Per Base Sequence Content: The proportion of each base position for which each of the four normal DNA bases has been called. +- Per Sequence GC Content: The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. +- Per Base N Content: The percentage of base calls at each position for which an N was called. +- Sequence Length Distribution. +- Sequence Duplication Levels: The relative level of duplication found for every sequence. +- Overrepresented sequences: The total amount of overrepresented sequences found in each library. +- Adapter Content: The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. + +![MultiQC - FASTQC Sequence Counts ](images/fastqc_seq_counts.png) +![MultiQC - FASTQC Mean Quality Distribution](images/fastqc_mean_qual_score_dist.png) +![MultiQC - FASTQC GC content](images/fastqc_gc_content.png) +![MultiQC - FASTQC Read Length Distribution](images/fastqc_length_dist.png) +![MultiQC - FASTQC Overall summary](images/fastqc_overall_status.png) + +#### GATK MarkDuplicates reports + +More information in the [GATK MarkDuplicates section](#gatk-markduplicates) + +Duplicates can arise during sample preparation *e.g.* library construction using PCR. +Duplicate reads can also result from a single amplification cluster, incorrectly detected as multiple clusters by the optical sensor of the sequencing instrument. These duplication artifacts are referred to as optical duplicates. + +
    +Output files + +- `reports/stats/[SAMPLE]/` + - `[SAMPLE].markdup.sorted.metrics`: Information about the number of duplicate reads in the sample. + +
    + +For further reading and documentation see the [MarkDuplicates manual](https://software.broadinstitute.org/gatk/documentation/tooldocs/4.1.2.0/picard_sam_markduplicates_MarkDuplicates.php). + +#### samtools stats + +[samtools stats](https://www.htslib.org/doc/samtools.html) collects statistics from `BAM` files and outputs in a text format. + +Plots will show: + +- Alignment metrics. + +
    +Output files + +- `results/reports/stats/[SAMPLE]/` + - `[SAMPLE].aligned.bam.flagstat`: Samtools flagstat output on raw alignment BAM. + - `[SAMPLE].aligned.bam.stats`: Samtools stats on raw alignment BAM. + - `[SAMPLE].markdup.sorted.bam.flagstat`: Samtools flagstat output on markduplicated BAM. + - `[SAMPLE].markdup.sorted.bam.stats`: Samtools flagstat output on markduplicated BAM. + - `[SAMPLE].recal.bam.stats`: Samtools flagstat output on recalibrated BAM. + +
    + +For further reading and documentation see the [`samtools` manual](https://www.htslib.org/doc/samtools.html#COMMANDS_AND_OPTIONS) + +#### snpEff reports + +[snpeff](http://snpeff.sourceforge.net/) is a genetic variant annotation and effect prediction toolbox. +It annotates and predicts the effects of variants on genes (such as amino acid changes) using multiple databases for annotations. + +Plots will shows : + +- locations of detected variants in the genome and the number of variants for each location. +- the putative impact of detected variants and the number of variants for each impact. +- the effect of variants at protein level and the number of variants for each effect type. +- the quantity as function of the variant quality score. + +
    +Output files + +- `results/reports/SnpEff/[SAMPLE]/` + - `[SAMPLE].csv`: Summary of variants by chromosome, region, effect, impact, functional class, type, etc. + - `[SAMPLE].genes.txt`: TXT (tab separated) summary counts for variants affecting each transcript and gene. + - `snpEff_summary.html`: Statistics with graphs to be viewed with a web browser + +
    + +#### VEP reports + +[VEP (Variant Effect Predictor)](https://www.ensembl.org/info/docs/tools/vep/index.html), based on `Ensembl`, is a tools to determine the effects of all sorts of variants, including SNPs, indels, structural variants, CNVs. + +
    +Output files + +- `results/reports/EnsemblVEP/[SAMPLE]/` + - `[SAMPLE].summary.html`: Statistics with graphs to be viewed with a web browser + +
    + +For further reading and documentation see the [VEP manual](https://www.ensembl.org/info/docs/tools/vep/index.html) + +### Reporting + +#### MultiQC + +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. +Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +![MultiQC - Report View](images/multiqc_report.png) -It integrates [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) which gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +The pipeline has special steps which also allow the software versions to be reported in the `MultiQC` output for future traceability. -The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . +For more information about how to use `MultiQC` reports, see [https://multiqc.info](https://multiqc.info).
    Output files - `reports/` - `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - - `multiqc_data/`: directory containing parsed statistics from FASTQC tools used in the pipeline. + - `multiqc_data/`: directory containing parsed statistics from different tools used in the pipeline. - `multiqc_plots/`: directory containing static images from the report in various formats.
    diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 010f8be1..70309891 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -10,7 +10,6 @@ include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/sam workflow RECALIBRATE { take: - skip_bamqc // boolean: true/false skip_samtools // boolean: true/false bam // channel: [mandatory] bam dict // channel: [mandatory] dict diff --git a/tests/test_annotation.yml b/tests/test_annotation.yml index d8855dc6..cf8fc787 100644 --- a/tests/test_annotation.yml +++ b/tests/test_annotation.yml @@ -8,24 +8,20 @@ - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi - path: results/reports/multiqc_report.html - name: Run VEP - command: nextflow run main.nf -profile test,docker --annotate_tools vep + command: nextflow run main.nf -profile test,docker --annotate_tools vep --skip_qc tags: - annotation - vep files: - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz.tbi - - path: results/reports/multiqc_report.html - name: Run snpEff followed by VEP - command: nextflow run main.nf -profile test,docker --annotate_tools merge + command: nextflow run main.nf -profile test,docker --annotate_tools merge --skip_qc tags: - annotation - merge - snpeff - vep files: - - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz - - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_snpEff_VEP.ann.vcf.gz.tbi - - path: results/reports/multiqc_report.html diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 02a3b29e..a8a76fae 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -37,8 +37,9 @@ if(!params.star_index && !params.gtf && !params.gff){ exit 1, "GTF|GFF3 file is ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true) +ch_multiqc_config = Channel.fromPath(file("$projectDir/assets/multiqc_config.yml", checkIfExists: true)) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() +ch_rnavar_logo = Channel.fromPath(file("$projectDir/assets/nf-core-rnavar_logo_light.png", checkIfExists: true)) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -117,6 +118,9 @@ def multiqc_report = [] workflow RNAVAR { + // To gather all QC reports for MultiQC + ch_reports = Channel.empty() + // To gather used softwares versions for MultiQC ch_versions = Channel.empty() // @@ -166,6 +170,7 @@ workflow RNAVAR { FASTQC ( ch_cat_fastq ) + ch_reports = ch_reports.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) // @@ -213,19 +218,19 @@ workflow RNAVAR { ch_genome_bam = ALIGN_STAR.out.bam ch_genome_bam_index = ALIGN_STAR.out.bai ch_transcriptome_bam = ALIGN_STAR.out.bam_transcript - ch_samtools_stats = ALIGN_STAR.out.stats - ch_samtools_flagstat = ALIGN_STAR.out.flagstat - ch_samtools_idxstats = ALIGN_STAR.out.idxstats - ch_star_multiqc = ALIGN_STAR.out.log_final + + // Gather QC reports + ch_reports = ch_reports.mix(ALIGN_STAR.out.stats.collect{it[1]}.ifEmpty([])) + ch_reports = ch_reports.mix(ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) // SUBWORKFLOW: Mark duplicates with GATK4 MARKDUPLICATES(ch_genome_bam) ch_genome_bam = MARKDUPLICATES.out.bam_bai - ch_samtools_stats = MARKDUPLICATES.out.stats - ch_samtools_flagstat = MARKDUPLICATES.out.flagstat - ch_samtools_idxstats = MARKDUPLICATES.out.idxstats - ch_markduplicates_multiqc = MARKDUPLICATES.out.metrics + + //Gather QC reports + ch_reports = ch_reports.mix(MARKDUPLICATES.out.stats.collect{it[1]}.ifEmpty([])) + ch_reports = ch_reports.mix(MARKDUPLICATES.out.metrics.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(MARKDUPLICATES.out.versions.first().ifEmpty(null)) // Subworkflow - SplitNCigarReads from GATK4 over the intervals @@ -260,6 +265,9 @@ workflow RNAVAR { known_sites_tbi ) ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table + + // Gather QC reports + ch_reports = ch_reports.mix(ch_bqsr_table.map{ meta, table -> table}) ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) // MODULE: ApplyBaseRecalibrator from GATK4 @@ -272,8 +280,7 @@ workflow RNAVAR { }.set{applybqsr_bam_bai_interval} RECALIBRATE( - ('bamqc' in params.skip_qc), - ('samtools' in params.skip_qc), + params.skip_qc, applybqsr_bam_bai_interval, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, @@ -282,6 +289,9 @@ workflow RNAVAR { bam_variant_calling = RECALIBRATE.out.bam bam_recalibrated_qc = RECALIBRATE.out.qc + + // Gather QC reports + ch_reports = ch_reports.mix(RECALIBRATE.out.qc.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) } else { bam_variant_calling = splitncigar_bam_bai @@ -369,33 +379,36 @@ workflow RNAVAR { vep_species, vep_cache_version, vep_cache) + + // Gather QC reports + ch_reports = ch_reports.mix(ANNOTATE.out.reports) ch_versions = ch_versions.mix(ANNOTATE.out.versions.first().ifEmpty(null)) } } - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + ch_version_yaml = Channel.empty() + CUSTOM_DUMPSOFTWAREVERSIONS (ch_versions.unique().collectFile(name: 'collated_versions.yml')) + ch_version_yaml = CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect() // // MODULE: MultiQC // - workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect() - ) - multiqc_report = MULTIQC.out.report.toList() - ch_versions = ch_versions.mix(MULTIQC.out.versions) + + if (!params.skip_qc){ + workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + ch_multiqc_files = Channel.empty().mix(ch_version_yaml, + ch_multiqc_custom_config.collect().ifEmpty([]), + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'), + ch_reports.collect(), + ch_multiqc_config, + ch_rnavar_logo) + + MULTIQC (ch_multiqc_files.collect()) + multiqc_report = MULTIQC.out.report.toList() + } + } /* From b7183506c050304d0f83efc5842bad2a59e577b7 Mon Sep 17 00:00:00 2001 From: Praveen Date: Thu, 12 May 2022 16:22:11 +0200 Subject: [PATCH 205/235] Fix: prettier issues --- assets/email_template.html | 141 ++++++++++++++++++++++++++----------- docs/output.md | 2 +- modules.json | 2 +- 3 files changed, 101 insertions(+), 44 deletions(-) diff --git a/assets/email_template.html b/assets/email_template.html index e8bda58a..ab86a4f3 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,53 +1,110 @@ - - - - + + + + - - nf-core/rnavar Pipeline Report - - -
    + + nf-core/rnavar Pipeline Report + + +
    + - +

    nf-core/rnavar v${version}

    +

    Run Name: $runName

    -

    nf-core/rnavar v${version}

    -

    Run Name: $runName

    - -<% if (!success){ - out << """ -
    -

    nf-core/rnavar execution completed unsuccessfully!

    + <% if (!success){ out << """ +
    +

    nf-core/rnavar execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    - 
    ${errorReport}
    -
    - """ -} else { - out << """ -
    + 
    ${errorReport}
    +
    + """ } else { out << """ +
    nf-core/rnavar execution completed successfully! -
    - """ -} -%> +
    + """ } %> -

    The workflow was completed at $dateComplete (duration: $duration)

    -

    The command used to launch the workflow was as follows:

    -
    $commandLine
    +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    + 
    +$commandLine
    -

    Pipeline Configuration:

    - - - <% out << summary.collect{ k,v -> "" }.join("\n") %> - -
    $k
    $v
    +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> " + + + + + " }.join("\n") %> + +
    + $k + + 
    $v
    +
    -

    nf-core/rnavar

    -

    https://github.com/nf-core/rnavar

    - -
    - - +

    nf-core/rnavar

    +

    https://github.com/nf-core/rnavar

    +
    + diff --git a/docs/output.md b/docs/output.md index 2084757b..690181b5 100644 --- a/docs/output.md +++ b/docs/output.md @@ -283,7 +283,7 @@ Plots will show: More information in the [GATK MarkDuplicates section](#gatk-markduplicates) -Duplicates can arise during sample preparation *e.g.* library construction using PCR. +Duplicates can arise during sample preparation _e.g._ library construction using PCR. Duplicate reads can also result from a single amplification cluster, incorrectly detected as multiple clusters by the optical sensor of the sequencing instrument. These duplication artifacts are referred to as optical duplicates.
    diff --git a/modules.json b/modules.json index 61e0b776..3f7997cc 100644 --- a/modules.json +++ b/modules.json @@ -98,4 +98,4 @@ } } } -} \ No newline at end of file +} From 88079ed443aefd3816b4b7636121dee36bfda6ed Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 17 May 2022 14:16:56 +0200 Subject: [PATCH 206/235] removed files_unchanged --- .nf-core.yml | 3 --- 1 file changed, 3 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index abfd2795..3805dc81 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,4 +1 @@ repository_type: pipeline -lint: - files_unchanged: - - assets/email_template.html From 7f15f58b298bf4c1bb0118f75841ebfdf64386dd Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 17 May 2022 14:17:43 +0200 Subject: [PATCH 207/235] fix: assets/email_template.html --- assets/email_template.html | 2 ++ 1 file changed, 2 insertions(+) diff --git a/assets/email_template.html b/assets/email_template.html index 21a09edc..e8bda58a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -3,11 +3,13 @@ + nf-core/rnavar Pipeline Report
    +

    nf-core/rnavar v${version}

    From b9dc6e72ebaac1c3af54768158439ef22480d619 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 17 May 2022 15:04:13 +0200 Subject: [PATCH 208/235] Corrected nextflow version in manifest - nextflow.config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 15257981..e3ab49f8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -235,7 +235,7 @@ manifest { homePage = 'https://github.com/nf-core/rnavar' description = 'GATK4 RNA variant calling pipeline' mainScript = 'main.nf' - nextflowVersion = '!>=21.10.3' + nextflowVersion = '>=21.10.3' version = '1.0dev' } From ba2893971d520fe95054eee06cd69a4b4c73162e Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 17 May 2022 16:10:07 +0200 Subject: [PATCH 209/235] Update: nf-core tools --- README.md | 2 +- modules.json | 24 +++++++++---------- .../nf-core/modules/gatk4/applybqsr/main.nf | 6 ++--- .../modules/gatk4/baserecalibrator/main.nf | 6 ++--- .../modules/gatk4/bedtointervallist/main.nf | 6 ++--- .../gatk4/createsequencedictionary/main.nf | 6 ++--- .../modules/gatk4/haplotypecaller/main.nf | 6 ++--- .../modules/gatk4/indexfeaturefile/main.nf | 6 ++--- .../modules/gatk4/intervallisttools/main.nf | 6 ++--- .../modules/gatk4/markduplicates/main.nf | 8 +++---- .../nf-core/modules/gatk4/mergevcfs/main.nf | 6 ++--- .../modules/gatk4/splitncigarreads/main.nf | 6 ++--- .../modules/gatk4/variantfiltration/main.nf | 6 ++--- modules/nf-core/modules/snpeff/Dockerfile | 9 +++---- modules/nf-core/modules/snpeff/build.sh | 5 ++-- modules/nf-core/modules/snpeff/meta.yml | 12 ---------- 16 files changed, 55 insertions(+), 65 deletions(-) diff --git a/README.md b/README.md index 56c31fbc..a00e8e23 100644 --- a/README.md +++ b/README.md @@ -48,7 +48,7 @@ On release, automated continuous integration tests run the pipeline on a full-si | FastQC | 0.11.9 | | STAR | 2.7.9a | | Samtools | 1.15.1 | -| GATK | 4.2.5.0 | +| GATK | 4.2.6.1 | | SnpEff | 5.0 | | Ensembl VEP | 104.3 | | MultiQC | 1.12 | diff --git a/modules.json b/modules.json index 3f7997cc..c35f0779 100644 --- a/modules.json +++ b/modules.json @@ -16,37 +16,37 @@ "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, "gatk4/applybqsr": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/baserecalibrator": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/bedtointervallist": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/createsequencedictionary": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/haplotypecaller": { - "git_sha": "68f1c27169946f931ea4318911de5681f88b2961" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/indexfeaturefile": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/intervallisttools": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/markduplicates": { - "git_sha": "0511e7fbbfa4ba41940d33b687b1cc90227b4eb8" + "git_sha": "df2620cfc7e4c21b14ed03c1c928f09fbabf83c4" }, "gatk4/mergevcfs": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/splitncigarreads": { - "git_sha": "10ca39a86f1b227a5535238d02398fec686eba72" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gatk4/variantfiltration": { - "git_sha": "409af2f27cbe45109acc7fee70718d2bf20aa449" + "git_sha": "169b2b96c1167f89ab07127b7057c1d90a6996c7" }, "gffread": { "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" @@ -79,7 +79,7 @@ "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, "snpeff": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" + "git_sha": "40dd662fd26c3eb3160b7c8cbbe9bff80bbe2c30" }, "star/align": { "git_sha": "1dddf1ce9443e3d93853d86e7a7aab52e5b4d614" diff --git a/modules/nf-core/modules/gatk4/applybqsr/main.nf b/modules/nf-core/modules/gatk4/applybqsr/main.nf index 7a64dab2..a0e2c45c 100644 --- a/modules/nf-core/modules/gatk4/applybqsr/main.nf +++ b/modules/nf-core/modules/gatk4/applybqsr/main.nf @@ -2,10 +2,10 @@ process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(bqsr_table), path(intervals) diff --git a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf index 766a8338..fb26d3da 100644 --- a/modules/nf-core/modules/gatk4/baserecalibrator/main.nf +++ b/modules/nf-core/modules/gatk4/baserecalibrator/main.nf @@ -2,10 +2,10 @@ process GATK4_BASERECALIBRATOR { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) diff --git a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf index 118f535b..6224d9b3 100644 --- a/modules/nf-core/modules/gatk4/bedtointervallist/main.nf +++ b/modules/nf-core/modules/gatk4/bedtointervallist/main.nf @@ -2,10 +2,10 @@ process GATK4_BEDTOINTERVALLIST { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(bed) diff --git a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf index dbf37048..13fa9e81 100644 --- a/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf +++ b/modules/nf-core/modules/gatk4/createsequencedictionary/main.nf @@ -2,10 +2,10 @@ process GATK4_CREATESEQUENCEDICTIONARY { tag "$fasta" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: path fasta diff --git a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf index 2cd9e7d4..6dd3f69e 100644 --- a/modules/nf-core/modules/gatk4/haplotypecaller/main.nf +++ b/modules/nf-core/modules/gatk4/haplotypecaller/main.nf @@ -2,10 +2,10 @@ process GATK4_HAPLOTYPECALLER { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(input), path(input_index), path(intervals) diff --git a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf index 90ff94e6..264f71ef 100644 --- a/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf +++ b/modules/nf-core/modules/gatk4/indexfeaturefile/main.nf @@ -2,10 +2,10 @@ process GATK4_INDEXFEATUREFILE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(feature_file) diff --git a/modules/nf-core/modules/gatk4/intervallisttools/main.nf b/modules/nf-core/modules/gatk4/intervallisttools/main.nf index 1b9b37f4..7ab26c15 100644 --- a/modules/nf-core/modules/gatk4/intervallisttools/main.nf +++ b/modules/nf-core/modules/gatk4/intervallisttools/main.nf @@ -2,10 +2,10 @@ process GATK4_INTERVALLISTTOOLS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(intervals) diff --git a/modules/nf-core/modules/gatk4/markduplicates/main.nf b/modules/nf-core/modules/gatk4/markduplicates/main.nf index 2650925b..68e4a21a 100644 --- a/modules/nf-core/modules/gatk4/markduplicates/main.nf +++ b/modules/nf-core/modules/gatk4/markduplicates/main.nf @@ -1,11 +1,11 @@ process GATK4_MARKDUPLICATES { tag "$meta.id" - label 'process_low' + label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/modules/gatk4/mergevcfs/main.nf b/modules/nf-core/modules/gatk4/mergevcfs/main.nf index 06ff3acb..964c1a3b 100644 --- a/modules/nf-core/modules/gatk4/mergevcfs/main.nf +++ b/modules/nf-core/modules/gatk4/mergevcfs/main.nf @@ -2,10 +2,10 @@ process GATK4_MERGEVCFS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(vcf) diff --git a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf index 85e5daa8..456ec055 100644 --- a/modules/nf-core/modules/gatk4/splitncigarreads/main.nf +++ b/modules/nf-core/modules/gatk4/splitncigarreads/main.nf @@ -2,10 +2,10 @@ process GATK4_SPLITNCIGARREADS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(bam), path(bai), path(intervals) diff --git a/modules/nf-core/modules/gatk4/variantfiltration/main.nf b/modules/nf-core/modules/gatk4/variantfiltration/main.nf index 6beb87ef..cda06e11 100644 --- a/modules/nf-core/modules/gatk4/variantfiltration/main.nf +++ b/modules/nf-core/modules/gatk4/variantfiltration/main.nf @@ -2,10 +2,10 @@ process GATK4_VARIANTFILTRATION { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::gatk4=4.2.5.0" : null) + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gatk4:4.2.5.0--hdfd78af_0' : - 'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" input: tuple val(meta), path(vcf), path(tbi) diff --git a/modules/nf-core/modules/snpeff/Dockerfile b/modules/nf-core/modules/snpeff/Dockerfile index 608716a4..d0e34757 100644 --- a/modules/nf-core/modules/snpeff/Dockerfile +++ b/modules/nf-core/modules/snpeff/Dockerfile @@ -8,15 +8,16 @@ LABEL \ COPY environment.yml / RUN conda env create -f /environment.yml && conda clean -a -# Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/nf-core-snpeff-5.0/bin:$PATH - # Setup default ARG variables ARG GENOME=GRCh38 ARG SNPEFF_CACHE_VERSION=99 +ARG SNPEFF_TAG=99 + +# Add conda installation dir to PATH (instead of doing 'conda activate') +ENV PATH /opt/conda/envs/nf-core-snpeff-${SNPEFF_TAG}/bin:$PATH # Download Genome RUN snpEff download -v ${GENOME}.${SNPEFF_CACHE_VERSION} # Dump the details of the installed packages to a file for posterity -RUN conda env export --name nf-core-snpeff-5.0 > nf-core-snpeff-5.0.yml +RUN conda env export --name nf-core-snpeff-${SNPEFF_TAG} > nf-core-snpeff-${SNPEFF_TAG}.yml diff --git a/modules/nf-core/modules/snpeff/build.sh b/modules/nf-core/modules/snpeff/build.sh index b94ffd69..2fccf9a8 100644 --- a/modules/nf-core/modules/snpeff/build.sh +++ b/modules/nf-core/modules/snpeff/build.sh @@ -9,10 +9,11 @@ build_push() { SNPEFF_TAG=$3 docker build \ + . \ -t nfcore/snpeff:${SNPEFF_TAG}.${GENOME} \ - software/snpeff/. \ --build-arg GENOME=${GENOME} \ - --build-arg SNPEFF_CACHE_VERSION=${SNPEFF_CACHE_VERSION} + --build-arg SNPEFF_CACHE_VERSION=${SNPEFF_CACHE_VERSION} \ + --build-arg SNPEFF_TAG=${SNPEFF_TAG} docker push nfcore/snpeff:${SNPEFF_TAG}.${GENOME} } diff --git a/modules/nf-core/modules/snpeff/meta.yml b/modules/nf-core/modules/snpeff/meta.yml index c191b9ac..2f0d866e 100644 --- a/modules/nf-core/modules/snpeff/meta.yml +++ b/modules/nf-core/modules/snpeff/meta.yml @@ -10,18 +10,6 @@ tools: homepage: https://pcingola.github.io/SnpEff/ documentation: https://pcingola.github.io/SnpEff/se_introduction/ licence: ["MIT"] -params: - - use_cache: - type: boolean - description: | - boolean to enable the usage of containers with cache - Enable the usage of containers with cache - Does not work with conda - - snpeff_tag: - type: value - description: | - Specify the tag for the container - https://hub.docker.com/r/nfcore/snpeff/tags input: - meta: type: map From 81976d0670af207639045ee90949984059c0eda5 Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 23 May 2022 16:31:11 +0200 Subject: [PATCH 210/235] Edits in documentations --- README.md | 3 ++- docs/usage.md | 10 +++++----- 2 files changed, 7 insertions(+), 6 deletions(-) diff --git a/README.md b/README.md index a00e8e23..090f92b6 100644 --- a/README.md +++ b/README.md @@ -36,7 +36,7 @@ On release, automated continuous integration tests run the pipeline on a full-si 9. Scatter one interval-list into many interval-files ([`GATK4 IntervalListTools`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917392155-IntervalListTools-Picard-)) 10. Call SNPs and indels ([`GATK4 HaplotypeCaller`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897180827-HaplotypeCaller)) 11. Merge multiple VCF files into one VCF ([`GATK4 MergeVCFs`](https://gatk.broadinstitute.org/hc/en-us/articles/4409924817691-MergeVcfs-Picard-)) -12. Index the VCF ([`GATK4 IndexFeatureFile`](https://gatk.broadinstitute.org/hc/en-us/articles/4409917499931-IndexFeatureFile)) +12. Index the VCF ([`Tabix`](http://www.htslib.org/doc/tabix.html)) 13. Filter variant calls based on certain criteria ([`GATK4 VariantFiltration`](https://gatk.broadinstitute.org/hc/en-us/articles/4409897204763-VariantFiltration)) 14. Annotate variants ([`snpEff`](https://pcingola.github.io/SnpEff/se_introduction/), [Ensembl VEP](https://www.ensembl.org/info/docs/tools/vep/index.html)) 15. Present QC for raw read, alignment, gene biotype, sample similarity, and strand-specificity checks ([`MultiQC`](http://multiqc.info/), [`R`](https://www.r-project.org/)) @@ -49,6 +49,7 @@ On release, automated continuous integration tests run the pipeline on a full-si | STAR | 2.7.9a | | Samtools | 1.15.1 | | GATK | 4.2.6.1 | +| Tabix | 1.11 | | SnpEff | 5.0 | | Ensembl VEP | 104.3 | | MultiQC | 1.12 | diff --git a/docs/usage.md b/docs/usage.md index 3f66258b..4d6b2cb0 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -256,11 +256,11 @@ Whilst the default requirements set within the pipeline will hopefully work for For example, if the nf-core/rnavar pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: ```console -[62/149eb0] NOTE: Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)' +[62/149eb0] NOTE: Process `NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) +Error executing process > 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)' Caused by: - Process `RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) + Process `NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) Command executed: STAR \ @@ -295,13 +295,13 @@ The custom config below can then be provided to the pipeline via the [`-c`](#-c) ```nextflow process { - withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' { + withName: 'NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:STAR_ALIGN' { memory = 100.GB } } ``` -> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. +> **NB:** We specify the full process name i.e. `NFCORE_RNAVAR:RNAVAR:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. > > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. From 3b53fe86e2a19bfb01a82cc44f3b0c4a8ea7ffa3 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 24 May 2022 12:03:50 +0200 Subject: [PATCH 211/235] Bump version to 1.0.0; Edit CHANGELOG.md --- CHANGELOG.md | 15 +++++++++++++-- nextflow.config | 2 +- 2 files changed, 14 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index cb967546..ba104592 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,12 +3,23 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0dev - [date] +## [1.0.0] nfcore/rnafusion - 2022/05/24 -Initial release of nf-core/rnavar, created with the [nf-core](https://nf-co.re/) template. +First production release of the pipeline with latest software versions. +It is written in Nextflow DSL2 and is based on the GATK4 best-practice pipeline for RNAseq [Ref](https://github.com/gatk-workflows/gatk4-rnaseq-germline-snps-indels). ### `Added` +- Added `FastQC v0.11.9` from nf-core modules for read-level QC and summary. +- Added `STAR v2.7.9a` from nf-core modules for read alignment to reference genome. +- Added `Samtools v1.15.1` from nf-core modules for alignment statistics and QC. +- Added `GATK v4.2.6.1` from nf-core modules for alignment post-processing, variant calling and filtration. +- Added `Tabix v1.11` from nf-core modules for indexing BAM ann VCF files. +- Added `SnpEff v5.0` from nf-core modules for variant annotation. +- Added `Ensembl VEP v104.3` from nf-core modules for variant annotation. +- Added `MultiQC v1.12` from nf-core modules for QC summary report. +- Added Scatter i.e., one interval-list into many interval-files to run multiple processes in parallel. + ### `Fixed` ### `Dependencies` diff --git a/nextflow.config b/nextflow.config index e3ab49f8..5c930df0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -236,7 +236,7 @@ manifest { description = 'GATK4 RNA variant calling pipeline' mainScript = 'main.nf' nextflowVersion = '>=21.10.3' - version = '1.0dev' + version = '1.0.0' } // Load modules.config for DSL2 module specific options From 1aff7bc35d790da6f099212dd8758c75cee989aa Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 24 May 2022 12:15:20 +0200 Subject: [PATCH 212/235] Edit: CHANGELOG.md --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ba104592..2cfd3f5a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [1.0.0] nfcore/rnafusion - 2022/05/24 +## [1.0.0] nfcore/rnavar - 2022/05/24 First production release of the pipeline with latest software versions. It is written in Nextflow DSL2 and is based on the GATK4 best-practice pipeline for RNAseq [Ref](https://github.com/gatk-workflows/gatk4-rnaseq-germline-snps-indels). From b5659563d23387b7e0a4a02eb897cc59355cc7ec Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 25 May 2022 11:47:05 +0200 Subject: [PATCH 213/235] Edit: CHANGELOG.md --- CHANGELOG.md | 10 ++++------ 1 file changed, 4 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2cfd3f5a..ba683a54 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,7 +6,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## [1.0.0] nfcore/rnavar - 2022/05/24 First production release of the pipeline with latest software versions. -It is written in Nextflow DSL2 and is based on the GATK4 best-practice pipeline for RNAseq [Ref](https://github.com/gatk-workflows/gatk4-rnaseq-germline-snps-indels). + +This version is based on GATK4 best-practices for RNAseq [Ref](https://github.com/gatk-workflows/gatk4-rnaseq-germline-snps-indels) and it includes: ### `Added` @@ -20,8 +21,5 @@ It is written in Nextflow DSL2 and is based on the GATK4 best-practice pipeline - Added `MultiQC v1.12` from nf-core modules for QC summary report. - Added Scatter i.e., one interval-list into many interval-files to run multiple processes in parallel. -### `Fixed` - -### `Dependencies` - -### `Deprecated` +Thanks to everyone that contributed to this release. +Special thanks to @maxulysse and @FriederikeHanssen for your review and valuable suggestions. From 249728ce06824d8e2dfe62db88ccee0a1bd49c2c Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 11:36:50 +0200 Subject: [PATCH 214/235] Fix: Incorrect merging of samples - Issue #54 --- bin/check_samplesheet.py | 17 +++++++---------- workflows/rnavar.nf | 6 ++++-- 2 files changed, 11 insertions(+), 12 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 8570149a..047b3fce 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -114,19 +114,16 @@ def validate_unique_samples(self): """ Assert that the combination of sample name and FASTQ filename is unique. - In addition to the validation, also rename the sample if more than one sample, - FASTQ file combination exists. + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ file(s). """ assert len(self._seen) == len(self.modified), "The pair of sample name and FASTQ must be unique." - if len({pair[0] for pair in self._seen}) < len(self._seen): - counts = Counter(pair[0] for pair in self._seen) - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - if counts[sample] > 1: - row[self._sample_col] = f"{sample}_T{seen[sample]}" + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" def read_head(handle, num_lines=10): diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index a8a76fae..b67c2cee 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -139,8 +139,10 @@ workflow RNAVAR { .reads .map { meta, fastq -> - meta.id = meta.id.split('_')[0..meta.id.split('_').size()-2].join('_') - [ meta, fastq ] } + def meta_clone = meta.clone() + meta_clone.id = meta_clone.id.split('_')[0..-2].join('_') + [ meta_clone, fastq ] + } .groupTuple(by: [0]) .branch { meta, fastq -> From 5a457b4fa7b893a4842032293404f46ba6e01620 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 11:39:42 +0200 Subject: [PATCH 215/235] Added: citation for Tabix in CITATIONS.md --- CITATIONS.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CITATIONS.md b/CITATIONS.md index d84e03ce..fedf7b37 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -36,7 +36,7 @@ > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. -- [Tabix]() +- [Tabix](https://pubmed.ncbi.nlm.nih.gov/21208982/) > Heng Li, Tabix: fast retrieval of sequence features from generic TAB-delimited files, Bioinformatics, Volume 27, Issue 5, 1 March 2011, Pages 718–719. doi: 10.1093/bioinformatics/btq671. PubMed PMID: 21208982; PubMed Central PMCID: PMC3042176. From b53e07f1d4d31741b6e0b61dbb4f6b37aa1cda76 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 11:41:13 +0200 Subject: [PATCH 216/235] Update: mode of GUNZIP_* in modules.config --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 721b4c05..45f8a678 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -51,7 +51,7 @@ process { withName: 'GUNZIP_.*' { publishDir = [ path: { "${params.outdir}/genome" }, - mode: 'copy', + mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, enabled: params.save_reference ] From 4881cdad9902b60acb30b8a0e3c409058fe145c9 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 14:34:09 +0200 Subject: [PATCH 217/235] Used multiline format for all process calls --- conf/modules.config | 20 ++--- docs/usage.md | 12 +-- nextflow.config | 88 ++++++++++----------- nextflow_schema.json | 18 ++--- subworkflows/local/annotate.nf | 22 +++++- subworkflows/local/input_check.nf | 12 +-- subworkflows/local/prepare_genome.nf | 38 +++++++-- subworkflows/nf-core/align_star.nf | 13 ++- subworkflows/nf-core/bam_sort_samtools.nf | 12 ++- subworkflows/nf-core/bam_stats_samtools.nf | 13 ++- subworkflows/nf-core/ensemblvep_annotate.nf | 13 ++- subworkflows/nf-core/markduplicates.nf | 12 ++- subworkflows/nf-core/recalibrate.nf | 11 ++- subworkflows/nf-core/snpeff_annotate.nf | 11 ++- subworkflows/nf-core/splitncigar.nf | 16 +++- tests/test_annotation.yml | 4 +- workflows/rnavar.nf | 29 +++++-- 17 files changed, 230 insertions(+), 114 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 45f8a678..5fca8a6a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -122,7 +122,7 @@ process { path: { "${params.outdir}/reports"}, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - enabled: !params.skip_qc + enabled: !params.skip_multiqc ] } @@ -206,7 +206,7 @@ process { '--SUBDIVISION_MODE BALANCING_WITHOUT_INTERVAL_SUBDIVISION_WITH_OVERFLOW', '--UNIQUE true', '--SORT true', - params.scatter_count ? "--SCATTER_COUNT $params.scatter_count" : '' + params.gatk_interval_scatter_count ? "--SCATTER_COUNT $params.gatk_interval_scatter_count" : '' ].join(' ').trim() publishDir = [ enabled: false ] } @@ -261,7 +261,7 @@ process { publishDir = [ path: { "${params.outdir}/reports/stats/${meta.id}" }, mode: params.publish_dir_mode, - enabled: !params.skip_qc, + enabled: !params.skip_multiqc, pattern: "*.{stats,flagstat}" ] } @@ -311,7 +311,7 @@ process { withName: GATK4_HAPLOTYPECALLER { ext.args = [ '--dont-use-soft-clipped-bases', - params.stand_call_conf ? "--standard-min-confidence-threshold-for-calling $params.stand_call_conf" : '', + params.gatk_hc_call_conf ? "--standard-min-confidence-threshold-for-calling $params.gatk_hc_call_conf" : '', params.bam_csi_index ? "--create-output-variant-index false" : "" ].join(' ').trim() publishDir = [ enabled: false ] @@ -340,10 +340,10 @@ process { withName: GATK4_VARIANTFILTRATION { ext.prefix = {"${meta.id}.haplotypecaller.filtered"} ext.args = [ - params.window ? "--window $params.window" : '', - params.cluster ? "--cluster $params.cluster" : '', - params.fs_filter ? "--filter-name \"FS\" --filter \"FS > $params.fs_filter\" " : '', - params.qd_filter ? "--filter-name \"QD\" --filter \"QD < $params.qd_filter\" " : '', + params.gatk_vf_window_size ? "--window $params.gatk_vf_window_size" : '', + params.gatk_vf_cluster_size ? "--cluster $params.gatk_vf_cluster_size" : '', + params.gatk_vf_fs_filter ? "--filter-name \"FS\" --filter \"FS > $params.gatk_vf_fs_filter\" " : '', + params.gatk_vf_qd_filter ? "--filter-name \"QD\" --filter \"QD < $params.gatk_vf_qd_filter\" " : '', ].join(' ').trim() publishDir = [ path: { "${params.outdir}/variant_calling/${meta.id}" }, @@ -366,7 +366,7 @@ process { mode: params.publish_dir_mode, path: { "${params.outdir}/reports/EnsemblVEP/${meta.id}" }, pattern: "*html", - enabled: !params.skip_qc + enabled: !params.skip_multiqc ] } @@ -380,7 +380,7 @@ process { path: { "${params.outdir}/reports/SnpEff/${meta.id}" }, pattern: "*.{csv,html,txt}", saveAs: { params.annotate_tools.contains('snpeff') ? it : null }, - enabled: !params.skip_qc + enabled: !params.skip_multiqc ] } diff --git a/docs/usage.md b/docs/usage.md index 4d6b2cb0..a59c1965 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -130,18 +130,18 @@ GATK best practices has been followed in this pipeline for RNA analysis, hence i > **NB:** Base recalibration can be turned off using `--skip_baserecalibration true` option. This is useful when you are analyzing data from non-model organisms where there is no known variant datasets exist. -`GATK SplitNCigarReads` is very time consuming step, therefore we made an attempt to break the GTF file into multiple chunks (scatters) using `GATK IntervalListTools` to run the process independently on each chunk in a parallel way to speed up the analysis. The default number of splits is set to 25, that means the GTF file is split into 25 smaller files and run `GATK SplitNCigarReads` on each of them in parallel. You can modify the number of splits using parameter `--scatter_count`. +`GATK SplitNCigarReads` is very time consuming step, therefore we made an attempt to break the GTF file into multiple chunks (scatters) using `GATK IntervalListTools` to run the process independently on each chunk in a parallel way to speed up the analysis. The default number of splits is set to 25, that means the GTF file is split into 25 smaller files and run `GATK SplitNCigarReads` on each of them in parallel. You can modify the number of splits using parameter `--gatk_interval_scatter_count`. ## Variant calling and filtering -`GATK HaplotypeCaller` is used for variant calling with default minimum phred-scaled confidence threshold as 20. This value can be changed using paramerter `--stand_call_conf`. +`GATK HaplotypeCaller` is used for variant calling with default minimum phred-scaled confidence threshold as 20. This value can be changed using paramerter `--gatk_hc_call_conf`. The pipeline runs a hard-filtering step on the variants by default. It does not filter out any variants, rather it flags i.e. PASS or other flags such as FS, QD, SnpCluster, etc. in FILTER column of the VCF. The following are the default filter criteria, however it can be changed using the respective parameters. -- `--cluster` is set to 3. It is the number of SNPs which make up a cluster. -- `--window` is set to 35. The window size (in bases) in which to evaluate clustered SNPs. -- `--fs_filter` is set to 30.0. Filter based on FisherStrand > 30.0. It is the Phred-scaled probability that there is strand bias at the site. -- `--qd_filter` is set to 2.0 meaning filter variants if Quality By Depth filter is < 2.0. +- `--gatk_vf_cluster_size` is set to 3. It is the number of SNPs which make up a cluster. +- `--gatk_vf_window_size` is set to 35. The window size (in bases) in which to evaluate clustered SNPs. +- `--gatk_vf_fs_filter` is set to 30.0. Filter based on FisherStrand > 30.0. It is the Phred-scaled probability that there is strand bias at the site. +- `--gatk_vf_qd_filter` is set to 2.0 meaning filter variants if Quality By Depth filter is < 2.0. Variant filtering is an optional step. You can skip it using `--skip_variantfiltration` parameter. diff --git a/nextflow.config b/nextflow.config index 5c930df0..007fded1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,72 +12,72 @@ params { // Pipeline options // Mandatory option - input = null // sample sheet + input = null // sample sheet // Genome and reference options - genome = 'GRCh38' - igenomes_base = 's3://ngi-igenomes/igenomes' - igenomes_ignore = false - save_reference = false - save_merged_fastq = false + genome = 'GRCh38' + igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_ignore = false + save_reference = false + save_merged_fastq = false // Sequence read information - read_length = 150 // Required for STAR to build index and align reads + read_length = 150 // Required for STAR to build index and align reads // Alignment - aligner = 'star' // Only STAR is currently supported. - star_twopass = true - star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR - star_limitBAMsortRAM = 0 - star_outBAMsortingBinsN = 50 - star_limitOutSJcollapsed = 1000000 - seq_center = null - seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work - bam_csi_index = false - save_unaligned = false - save_align_intermeds = false + aligner = 'star' // Only STAR is currently supported. + star_twopass = true + star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR + star_limitBAMsortRAM = 0 + star_outBAMsortingBinsN = 50 + star_limitOutSJcollapsed = 1000000 + seq_center = null + seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work + bam_csi_index = false + save_unaligned = false + save_align_intermeds = false // Preprocessing of alignment - remove_duplicates = false + remove_duplicates = false // Variant calling - no_intervals = false + no_intervals = false // Variant annotation - annotate_tools = null // List of annotation tools to run - snpeff or vep or merge - annotation_cache = false // Annotation cache disabled - cadd_cache = null // CADD cache disabled - cadd_indels = null // No CADD InDels file - cadd_indels_tbi = null // No CADD InDels index - cadd_wg_snvs = null // No CADD SNVs file - cadd_wg_snvs_tbi = null // No CADD SNVs index - genesplicer = null // genesplicer disabled within VEP - snpeff_cache = null // No directory for snpEff cache - snpeff_db = null // No default db for snpeff - vep_cache = null // No directory for VEP cache - vep_genome = null // No default genome for VEP - vep_cache_version = null // No default cache version for VEP + annotate_tools = null // List of annotation tools to run - snpeff or vep or merge + annotation_cache = false // Annotation cache disabled + cadd_cache = null // CADD cache disabled + cadd_indels = null // No CADD InDels file + cadd_indels_tbi = null // No CADD InDels index + cadd_wg_snvs = null // No CADD SNVs file + cadd_wg_snvs_tbi = null // No CADD SNVs index + genesplicer = null // genesplicer disabled within VEP + snpeff_cache = null // No directory for snpEff cache + snpeff_db = null // No default db for snpeff + vep_cache = null // No directory for VEP cache + vep_genome = null // No default genome for VEP + vep_cache_version = null // No default cache version for VEP // Skip steps - skip_baserecalibration = false - skip_intervallisttools = false - skip_variantfiltration = false - skip_variantannotation = false + skip_baserecalibration = false + skip_intervallisttools = false + skip_variantfiltration = false + skip_variantannotation = false // GATK intervallist parameters - scatter_count = 25 + gatk_interval_scatter_count = 25 //GATK haplotypecaller parameters - stand_call_conf = 20 + gatk_hc_call_conf = 20 //GATK variant filter parameters - window = 35 - cluster = 3 - fs_filter = 30.0 - qd_filter = 2.0 + gatk_vf_window_size = 35 + gatk_vf_cluster_size = 3 + gatk_vf_fs_filter = 30.0 + gatk_vf_qd_filter = 2.0 // QC - skip_qc = false + skip_multiqc = false // MultiQC options multiqc_config = null diff --git a/nextflow_schema.json b/nextflow_schema.json index fa2466b9..57a4f0e7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -253,7 +253,7 @@ "default": "", "fa_icon": "fas fa-toolbox", "properties": { - "stand_call_conf": { + "gatk_hc_call_conf": { "type": "number", "default": 20, "fa_icon": "fas fa-hammer", @@ -363,10 +363,10 @@ "description": "Skip variant annotation", "help_text": "Set this parameter if you don't want to run variant annotation." }, - "skip_qc": { + "skip_multiqc": { "type": "boolean", - "description": "Skip QC reports", - "help_text": "This parameter disable all QC reports from recalibrated BAM file." + "description": "Skip MultiQC reports", + "help_text": "This parameter disable all QC reports" } } }, @@ -376,7 +376,7 @@ "fa_icon": "fas fa-terminal", "description": "Define parameters of the tools used in the pipeline", "properties": { - "scatter_count": { + "gatk_interval_scatter_count": { "type": "integer", "default": 25, "description": "Number of times the gene interval list to be split in order to run GATK haplotype caller in parallel", @@ -387,25 +387,25 @@ "description": "Do not use gene interval file during variant calling", "help_text": "This parameter, if set to True, does not use the gene intervals during the variant calling step, which then results in variants from all regions including non-genic. Default is False" }, - "window": { + "gatk_vf_window_size": { "type": "integer", "default": 35, "description": "The window size (in bases) in which to evaluate clustered SNPs.", "help_text": "This parameter is used by GATK variant filteration step. It defines the window size (in bases) in which to evaluate clustered SNPs. It has to be used together with the other option 'cluster'." }, - "cluster": { + "gatk_vf_cluster_size": { "type": "integer", "default": 3, "description": "The number of SNPs which make up a cluster. Must be at least 2.", "help_text": "This parameter is used by GATK variant filteration step. It defines the number of SNPs which make up a cluster within a window. Must be at least 2." }, - "fs_filter": { + "gatk_vf_fs_filter": { "type": "number", "default": 30, "description": "Value to be used for the FisherStrand (FS) filter", "help_text": "This parameter defines the value to use for the FisherStrand (FS) filter in the GATK variant-filtering step. \nThe value should given in a float number format. Default is 30.0" }, - "qd_filter": { + "gatk_vf_qd_filter": { "type": "number", "default": 2, "description": "Value to be used for the QualByDepth (QD) filter", diff --git a/subworkflows/local/annotate.nf b/subworkflows/local/annotate.nf index 4c7b03ab..9555dddb 100644 --- a/subworkflows/local/annotate.nf +++ b/subworkflows/local/annotate.nf @@ -24,7 +24,11 @@ workflow ANNOTATE { ch_vcf_ann = Channel.empty() if (tools.contains('snpeff') || tools.contains('merge')) { - SNPEFF_ANNOTATE(vcf, snpeff_db, snpeff_cache) + SNPEFF_ANNOTATE ( + vcf, + snpeff_db, + snpeff_cache + ) ch_vcf_ann = ch_vcf_ann.mix(SNPEFF_ANNOTATE.out.vcf_tbi) ch_reports = ch_reports.mix(SNPEFF_ANNOTATE.out.reports) ch_versions = ch_versions.mix(SNPEFF_ANNOTATE.out.versions.first()) @@ -32,14 +36,26 @@ workflow ANNOTATE { if (tools.contains('merge')) { vcf_ann_for_merge = SNPEFF_ANNOTATE.out.vcf_tbi.map{ meta, vcf, tbi -> [meta, vcf] } - MERGE_ANNOTATE(vcf_ann_for_merge, vep_genome, vep_species, vep_cache_version, vep_cache) + MERGE_ANNOTATE ( + vcf_ann_for_merge, + vep_genome, + vep_species, + vep_cache_version, + vep_cache + ) ch_vcf_ann = ch_vcf_ann.mix(MERGE_ANNOTATE.out.vcf_tbi) ch_reports = ch_reports.mix(MERGE_ANNOTATE.out.reports) ch_versions = ch_versions.mix(MERGE_ANNOTATE.out.versions.first()) } if (tools.contains('vep')) { - ENSEMBLVEP_ANNOTATE(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + ENSEMBLVEP_ANNOTATE ( + vcf, + vep_genome, + vep_species, + vep_cache_version, + vep_cache + ) ch_vcf_ann = ch_vcf_ann.mix(ENSEMBLVEP_ANNOTATE.out.vcf_tbi) ch_reports = ch_reports.mix(ENSEMBLVEP_ANNOTATE.out.reports) ch_versions = ch_versions.mix(ENSEMBLVEP_ANNOTATE.out.versions.first()) diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 0aecf87f..95fc458e 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -9,11 +9,13 @@ workflow INPUT_CHECK { samplesheet // file: /path/to/samplesheet.csv main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } + SAMPLESHEET_CHECK ( + samplesheet + ) + .csv + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channel(it) } + .set { reads } emit: reads // channel: [ val(meta), [ reads ] ] diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index b0721aaf..0802bd04 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -26,7 +26,9 @@ workflow PREPARE_GENOME { // Uncompress genome fasta file if required // if (params.fasta.endsWith('.gz')) { - GUNZIP_FASTA ( Channel.fromPath(params.fasta).map{ it -> [[id:it[0].baseName], it] } ) + GUNZIP_FASTA ( + Channel.fromPath(params.fasta).map{ it -> [[id:it[0].baseName], it] } + ) ch_fasta = GUNZIP_FASTA.out.gunzip.map{ meta, fasta -> [fasta] }.collect() ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { @@ -40,7 +42,9 @@ workflow PREPARE_GENOME { ch_gffread_version = Channel.empty() if (params.gtf) { if (params.gtf.endsWith('.gz')) { - GUNZIP_GTF ( Channel.fromPath(params.gtf).map{ it -> [[id:it[0].baseName], it] } ) + GUNZIP_GTF ( + Channel.fromPath(params.gtf).map{ it -> [[id:it[0].baseName], it] } + ) ch_gtf = GUNZIP_GTF.out.gunzip.map{ meta, gtf -> [gtf] }.collect() ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { @@ -48,13 +52,21 @@ workflow PREPARE_GENOME { } } else if (params.gff) { if (params.gff.endsWith('.gz')) { - GUNZIP_GFF ( Channel.fromPath(params.gff).map{ it -> [[id:it[0].baseName], it] } ) + GUNZIP_GFF ( + Channel.fromPath(params.gff).map{ it -> [[id:it[0].baseName], it] } + ) ch_gff = GUNZIP_GFF.out.gunzip.map{ meta, gff -> [gff] }.collect() ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions) } else { ch_gff = Channel.fromPath(params.gff).collect() } - ch_gtf = GFFREAD ( ch_gff ).gtf + + GFFREAD ( + ch_gff + ) + .gtf + .set { ch_gtf } + ch_versions = ch_versions.mix(GFFREAD.out.versions) } @@ -63,7 +75,9 @@ workflow PREPARE_GENOME { // if (params.exon_bed) { if (params.exon_bed.endsWith('.gz')) { - GUNZIP_GENE_BED ( Channel.fromPath(params.exon_bed).map{ it -> [[id:it[0].baseName], it] } ) + GUNZIP_GENE_BED ( + Channel.fromPath(params.exon_bed).map{ it -> [[id:it[0].baseName], it] } + ) ch_gene_bed = GUNZIP_GENE_BED.out.gunzip.map{ meta, bed -> [bed] }.collect() ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { @@ -78,7 +92,9 @@ workflow PREPARE_GENOME { ch_fasta_fai = Channel.empty() if (params.fasta_fai) ch_fasta_fai = Channel.fromPath(params.fasta_fai).collect() if (!params.fasta_fai) { - SAMTOOLS_FAIDX(ch_fasta.map{ it -> [[id:it[0].getName()], it]}) + SAMTOOLS_FAIDX( + ch_fasta.map{ it -> [[id:it[0].getName()], it]} + ) ch_fasta_fai = SAMTOOLS_FAIDX.out.fai.map{ meta, fai -> [fai] }.collect() ch_versions = ch_versions.mix(SAMTOOLS_FAIDX.out.versions) } @@ -97,7 +113,9 @@ workflow PREPARE_GENOME { if ('star' in prepare_tool_indices) { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { - UNTAR_STAR_INDEX (Channel.fromPath(params.star_index).map{ it -> [[id:it[0].baseName], it] }) + UNTAR_STAR_INDEX ( + Channel.fromPath(params.star_index).map{ it -> [[id:it[0].baseName], it] } + ) ch_star_index = UNTAR_STAR_INDEX.out.untar.map{ meta, star_index -> [star_index] }.collect() ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { @@ -105,7 +123,11 @@ workflow PREPARE_GENOME { } } else { - ch_star_index = STAR_GENOMEGENERATE(ch_fasta,ch_gtf).index + STAR_GENOMEGENERATE ( + ch_fasta,ch_gtf + ) + .index + .set { ch_star_index } ch_versions = ch_versions.mix(STAR_GENOMEGENERATE.out.versions) } diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf index 87e482db..2a4069fd 100644 --- a/subworkflows/nf-core/align_star.nf +++ b/subworkflows/nf-core/align_star.nf @@ -21,13 +21,22 @@ workflow ALIGN_STAR { // // Map reads with STAR // - STAR_ALIGN ( reads, index, gtf, star_ignore_sjdbgtf, seq_platform, seq_center ) + STAR_ALIGN ( + reads, + index, + gtf, + star_ignore_sjdbgtf, + seq_platform, + seq_center + ) ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first()) // // Sort, index BAM file and run samtools stats, flagstat and idxstats // - BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) + BAM_SORT_SAMTOOLS ( + STAR_ALIGN.out.bam + ) ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions.first()) emit: diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index d735bd37..f473b759 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -14,10 +14,14 @@ workflow BAM_SORT_SAMTOOLS { ch_versions = Channel.empty() - SAMTOOLS_SORT ( ch_bam ) + SAMTOOLS_SORT ( + ch_bam + ) ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) - SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + SAMTOOLS_INDEX ( + SAMTOOLS_SORT.out.bam + ) ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) SAMTOOLS_SORT.out.bam @@ -33,7 +37,9 @@ workflow BAM_SORT_SAMTOOLS { } .set { ch_bam_bai } - BAM_STATS_SAMTOOLS ( ch_bam_bai ) + BAM_STATS_SAMTOOLS ( + ch_bam_bai + ) ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) emit: diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index 3d505a4d..68d632c3 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -13,13 +13,20 @@ workflow BAM_STATS_SAMTOOLS { main: ch_versions = Channel.empty() - SAMTOOLS_STATS ( ch_bam_bai, [] ) + SAMTOOLS_STATS ( + ch_bam_bai, + [] + ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) - SAMTOOLS_FLAGSTAT ( ch_bam_bai ) + SAMTOOLS_FLAGSTAT ( + ch_bam_bai + ) ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions.first()) - SAMTOOLS_IDXSTATS ( ch_bam_bai ) + SAMTOOLS_IDXSTATS ( + ch_bam_bai + ) ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions.first()) emit: diff --git a/subworkflows/nf-core/ensemblvep_annotate.nf b/subworkflows/nf-core/ensemblvep_annotate.nf index 40572a8b..13dffee4 100644 --- a/subworkflows/nf-core/ensemblvep_annotate.nf +++ b/subworkflows/nf-core/ensemblvep_annotate.nf @@ -17,9 +17,18 @@ workflow ENSEMBLVEP_ANNOTATE { ch_versions = Channel.empty() - ENSEMBLVEP(vcf, vep_genome, vep_species, vep_cache_version, vep_cache) + ENSEMBLVEP ( + vcf, + vep_genome, + vep_species, + vep_cache_version, + vep_cache + ) ch_versions = ch_versions.mix(ENSEMBLVEP.out.versions.first()) - TABIX_BGZIPTABIX(ENSEMBLVEP.out.vcf) + + TABIX_BGZIPTABIX ( + ENSEMBLVEP.out.vcf + ) ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) emit: diff --git a/subworkflows/nf-core/markduplicates.nf b/subworkflows/nf-core/markduplicates.nf index b1c41edc..85b724dd 100644 --- a/subworkflows/nf-core/markduplicates.nf +++ b/subworkflows/nf-core/markduplicates.nf @@ -14,13 +14,17 @@ workflow MARKDUPLICATES { ch_versions = Channel.empty() - GATK4_MARKDUPLICATES(bam) + GATK4_MARKDUPLICATES ( + bam + ) ch_versions = ch_versions.mix(GATK4_MARKDUPLICATES.out.versions.first()) // // Index BAM file and run samtools stats, flagstat and idxstats // - SAMTOOLS_INDEX(GATK4_MARKDUPLICATES.out.bam) + SAMTOOLS_INDEX ( + GATK4_MARKDUPLICATES.out.bam + ) ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) GATK4_MARKDUPLICATES.out.bam @@ -31,7 +35,9 @@ workflow MARKDUPLICATES { else [meta, bam, csi]} .set{ch_bam_bai} - BAM_STATS_SAMTOOLS(ch_bam_bai) + BAM_STATS_SAMTOOLS ( + ch_bam_bai + ) ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) emit: diff --git a/subworkflows/nf-core/recalibrate.nf b/subworkflows/nf-core/recalibrate.nf index 70309891..664cf3f5 100644 --- a/subworkflows/nf-core/recalibrate.nf +++ b/subworkflows/nf-core/recalibrate.nf @@ -24,11 +24,18 @@ workflow RECALIBRATE { bam_recalibrated = Channel.empty() bam_reports = Channel.empty() - APPLYBQSR(bam, fasta, fai, dict) + APPLYBQSR ( + bam, + fasta, + fai, + dict + ) bam_recalibrated = APPLYBQSR.out.bam ch_versions = ch_versions.mix(APPLYBQSR.out.versions.first()) - SAMTOOLS_INDEX(bam_recalibrated) + SAMTOOLS_INDEX ( + bam_recalibrated + ) bam_recalibrated_index = bam_recalibrated .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) diff --git a/subworkflows/nf-core/snpeff_annotate.nf b/subworkflows/nf-core/snpeff_annotate.nf index b7b618d0..9a8b65bc 100644 --- a/subworkflows/nf-core/snpeff_annotate.nf +++ b/subworkflows/nf-core/snpeff_annotate.nf @@ -15,9 +15,16 @@ workflow SNPEFF_ANNOTATE { ch_versions = Channel.empty() - SNPEFF(vcf, snpeff_db, snpeff_cache) + SNPEFF ( + vcf, + snpeff_db, + snpeff_cache + ) ch_versions = ch_versions.mix(SNPEFF.out.versions.first()) - TABIX_BGZIPTABIX(SNPEFF.out.vcf) + + TABIX_BGZIPTABIX ( + SNPEFF.out.vcf + ) ch_versions = ch_versions.mix(TABIX_BGZIPTABIX.out.versions.first()) emit: diff --git a/subworkflows/nf-core/splitncigar.nf b/subworkflows/nf-core/splitncigar.nf index 150c5bc1..1b1895f7 100644 --- a/subworkflows/nf-core/splitncigar.nf +++ b/subworkflows/nf-core/splitncigar.nf @@ -26,7 +26,12 @@ workflow SPLITNCIGAR { [new_meta, bam, bai, intervals] }.set{bam_interval} - GATK4_SPLITNCIGARREADS(bam_interval, fasta, fasta_fai, fasta_dict) + GATK4_SPLITNCIGARREADS ( + bam_interval, + fasta, + fasta_fai, + fasta_dict + ) bam_splitncigar = GATK4_SPLITNCIGARREADS.out.bam ch_versions = ch_versions.mix(GATK4_SPLITNCIGARREADS.out.versions.first()) @@ -37,11 +42,16 @@ workflow SPLITNCIGAR { [new_meta, bam] }.groupTuple().set{bam_splitncigar_interval} - SAMTOOLS_MERGE(bam_splitncigar_interval, fasta) + SAMTOOLS_MERGE ( + bam_splitncigar_interval, + fasta + ) splitncigar_bam = SAMTOOLS_MERGE.out.bam ch_versions = ch_versions.mix(SAMTOOLS_MERGE.out.versions.first()) - SAMTOOLS_INDEX(splitncigar_bam) + SAMTOOLS_INDEX ( + splitncigar_bam + ) splitncigar_bam_bai = splitncigar_bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) diff --git a/tests/test_annotation.yml b/tests/test_annotation.yml index cf8fc787..b6fb529d 100644 --- a/tests/test_annotation.yml +++ b/tests/test_annotation.yml @@ -8,7 +8,7 @@ - path: results/variant_annotation/GM12878/GM12878_snpEff.ann.vcf.gz.tbi - path: results/reports/multiqc_report.html - name: Run VEP - command: nextflow run main.nf -profile test,docker --annotate_tools vep --skip_qc + command: nextflow run main.nf -profile test,docker --annotate_tools vep --skip_multiqc tags: - annotation - vep @@ -16,7 +16,7 @@ - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz - path: results/variant_annotation/GM12878/GM12878_VEP.ann.vcf.gz.tbi - name: Run snpEff followed by VEP - command: nextflow run main.nf -profile test,docker --annotate_tools merge --skip_qc + command: nextflow run main.nf -profile test,docker --annotate_tools merge --skip_multiqc tags: - annotation - merge diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index b67c2cee..b59e6391 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -126,7 +126,9 @@ workflow RNAVAR { // // SUBWORKFLOW: Uncompress and prepare reference genome files // - PREPARE_GENOME (prepareToolIndices) + PREPARE_GENOME ( + prepareToolIndices + ) ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.exon_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) @@ -180,7 +182,10 @@ workflow RNAVAR { // ch_interval_list = Channel.empty() - GATK4_BEDTOINTERVALLIST(ch_genome_bed, PREPARE_GENOME.out.dict) + GATK4_BEDTOINTERVALLIST( + ch_genome_bed, + PREPARE_GENOME.out.dict + ) ch_interval_list = GATK4_BEDTOINTERVALLIST.out.interval_list ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions.first().ifEmpty(null)) @@ -190,7 +195,9 @@ workflow RNAVAR { ch_interval_list_split = Channel.empty() if (!params.skip_intervallisttools) { - GATK4_INTERVALLISTTOOLS(ch_interval_list) + GATK4_INTERVALLISTTOOLS( + ch_interval_list + ) ch_interval_list_split = GATK4_INTERVALLISTTOOLS.out.interval_list.map{ meta, bed -> [bed] }.flatten() } else ch_interval_list_split = ch_interval_list @@ -227,7 +234,9 @@ workflow RNAVAR { ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) // SUBWORKFLOW: Mark duplicates with GATK4 - MARKDUPLICATES(ch_genome_bam) + MARKDUPLICATES ( + ch_genome_bam + ) ch_genome_bam = MARKDUPLICATES.out.bam_bai //Gather QC reports @@ -238,7 +247,13 @@ workflow RNAVAR { // Subworkflow - SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). splitncigar_bam_bai = Channel.empty() - SPLITNCIGAR(ch_genome_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, ch_interval_list_split) + SPLITNCIGAR ( + ch_genome_bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_interval_list_split + ) splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) @@ -282,7 +297,7 @@ workflow RNAVAR { }.set{applybqsr_bam_bai_interval} RECALIBRATE( - params.skip_qc, + params.skip_multiqc, applybqsr_bam_bai_interval, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, @@ -397,7 +412,7 @@ workflow RNAVAR { // MODULE: MultiQC // - if (!params.skip_qc){ + if (!params.skip_multiqc){ workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty().mix(ch_version_yaml, From bb05debc01b914c059174f4af998ac14c7e0483f Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 15:14:18 +0200 Subject: [PATCH 218/235] Multiline formatting, ch_ prefix to all channels --- conf/test.config | 2 +- tests/csv/1.0/samplesheet.csv | 3 - workflows/rnavar.nf | 110 +++++++++++++++++----------------- 3 files changed, 56 insertions(+), 59 deletions(-) delete mode 100644 tests/csv/1.0/samplesheet.csv diff --git a/conf/test.config b/conf/test.config index e931a9a8..dd25aa41 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = "${baseDir}/tests/csv/1.0/samplesheet.csv" + input = "https://raw.githubusercontent.com/nf-core/test-datasets/rnavar/samplesheet/v1.0/samplesheet.csv" // Genome references genome = 'WBcel235' diff --git a/tests/csv/1.0/samplesheet.csv b/tests/csv/1.0/samplesheet.csv deleted file mode 100644 index d6a719d9..00000000 --- a/tests/csv/1.0/samplesheet.csv +++ /dev/null @@ -1,3 +0,0 @@ -sample,fastq_1,fastq_2,strandedness -GM12878,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse -TEST123,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz,https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz,reverse diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index b59e6391..69a5ed4b 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -94,18 +94,18 @@ def seq_platform = params.seq_platform ? params.seq_platform : [] def seq_center = params.seq_center ? params.seq_center : [] // Initialize file channels based on params -dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : Channel.empty() -dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : Channel.empty() -known_indels = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() -known_indels_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() +ch_dbsnp = params.dbsnp ? Channel.fromPath(params.dbsnp).collect() : Channel.empty() +ch_dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbsnp_tbi).collect() : Channel.empty() +ch_known_indels = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() +ch_known_indels_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() // Initialize varaint annotation associated channels -def snpeff_db = params.snpeff_db ?: Channel.empty() -def vep_cache_version = params.vep_cache_version ?: Channel.empty() -def vep_genome = params.vep_genome ?: Channel.empty() -def vep_species = params.vep_species ?: Channel.empty() -def snpeff_cache = params.snpeff_cache ? Channel.fromPath(params.snpeff_cache).collect() : [] -def vep_cache = params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : [] +ch_snpeff_db = params.snpeff_db ?: Channel.empty() +ch_vep_cache_version = params.vep_cache_version ?: Channel.empty() +ch_vep_genome = params.vep_genome ?: Channel.empty() +ch_vep_species = params.vep_species ?: Channel.empty() +ch_snpeff_cache = params.snpeff_cache ? Channel.fromPath(params.snpeff_cache).collect() : [] +ch_vep_cache = params.vep_cache ? Channel.fromPath(params.vep_cache).collect() : [] // MultiQC reporting def multiqc_report = [] @@ -246,7 +246,7 @@ workflow RNAVAR { // Subworkflow - SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). - splitncigar_bam_bai = Channel.empty() + ch_splitncigar_bam_bai = Channel.empty() SPLITNCIGAR ( ch_genome_bam, PREPARE_GENOME.out.fasta, @@ -254,10 +254,10 @@ workflow RNAVAR { PREPARE_GENOME.out.dict, ch_interval_list_split ) - splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai - ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) + ch_splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai + ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) - bam_variant_calling = Channel.empty() + ch_bam_variant_calling = Channel.empty() if(!params.skip_baserecalibration) { // MODULE: BaseRecalibrator from GATK4 @@ -265,21 +265,21 @@ workflow RNAVAR { // known_sites is made by grouping both the dbsnp and the known indels ressources // they can either or both be optional - known_sites = dbsnp.concat(known_indels).collect() - known_sites_tbi = dbsnp_tbi.concat(known_indels_tbi).collect() + ch_known_sites = ch_dbsnp.concat(ch_known_indels).collect() + ch_known_sites_tbi = ch_dbsnp_tbi.concat(ch_known_indels_tbi).collect() ch_interval_list_recalib = ch_interval_list.map{ meta, bed -> [bed] }.flatten() - splitncigar_bam_bai.combine(ch_interval_list_recalib) + ch_splitncigar_bam_bai.combine(ch_interval_list_recalib) .map{ meta, bam, bai, interval -> [ meta, bam, bai, interval] - }.set{splitncigar_bam_bai_interval} + }.set{ch_splitncigar_bam_bai_interval} GATK4_BASERECALIBRATOR( - splitncigar_bam_bai_interval, + ch_splitncigar_bam_bai_interval, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, - known_sites, - known_sites_tbi + ch_known_sites, + ch_known_sites_tbi ) ch_bqsr_table = GATK4_BASERECALIBRATOR.out.table @@ -288,43 +288,43 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) // MODULE: ApplyBaseRecalibrator from GATK4 - bam_applybqsr = splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) - bam_recalibrated_qc = Channel.empty() + ch_bam_applybqsr = ch_splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) + ch_bam_recalibrated_qc = Channel.empty() ch_interval_list_applybqsr = ch_interval_list.map{ meta, bed -> [bed] }.flatten() - bam_applybqsr.combine(ch_interval_list_applybqsr) + ch_bam_applybqsr.combine(ch_interval_list_applybqsr) .map{ meta, bam, bai, table, interval -> [ meta, bam, bai, table, interval] - }.set{applybqsr_bam_bai_interval} + }.set{ch_applybqsr_bam_bai_interval} RECALIBRATE( params.skip_multiqc, - applybqsr_bam_bai_interval, + ch_applybqsr_bam_bai_interval, PREPARE_GENOME.out.dict, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.fasta ) - bam_variant_calling = RECALIBRATE.out.bam - bam_recalibrated_qc = RECALIBRATE.out.qc + ch_bam_variant_calling = RECALIBRATE.out.bam + ch_bam_recalibrated_qc = RECALIBRATE.out.qc // Gather QC reports - ch_reports = ch_reports.mix(RECALIBRATE.out.qc.collect{it[1]}.ifEmpty([])) - ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) + ch_reports = ch_reports.mix(RECALIBRATE.out.qc.collect{it[1]}.ifEmpty([])) + ch_versions = ch_versions.mix(RECALIBRATE.out.versions.first().ifEmpty(null)) } else { - bam_variant_calling = splitncigar_bam_bai + ch_bam_variant_calling = ch_splitncigar_bam_bai } // MODULE: HaplotypeCaller from GATK4 interval_flag = params.no_intervals // Run haplotyper even in the absence of dbSNP files if (!params.dbsnp){ - dbsnp = [] - dbsnp_tbi = [] + ch_dbsnp = [] + ch_dbsnp_tbi = [] } - haplotypecaller_vcf = Channel.empty() + ch_haplotypecaller_vcf = Channel.empty() - haplotypecaller_interval_bam = bam_variant_calling.combine(ch_interval_list_split) + ch_haplotypecaller_interval_bam = ch_bam_variant_calling.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() new_meta.id = meta.id + "_" + interval_list.baseName @@ -333,15 +333,15 @@ workflow RNAVAR { } GATK4_HAPLOTYPECALLER( - haplotypecaller_interval_bam, + ch_haplotypecaller_interval_bam, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict, - dbsnp, - dbsnp_tbi + ch_dbsnp, + ch_dbsnp_tbi ) - haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf + ch_haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf .map{ meta, vcf -> meta.id = meta.sample [meta, vcf]} @@ -350,17 +350,17 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLER.out.versions.first().ifEmpty(null)) GATK4_MERGEVCFS( - haplotypecaller_raw, + ch_haplotypecaller_raw, PREPARE_GENOME.out.dict ) - haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf + ch_haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) TABIX( - haplotypecaller_vcf + ch_haplotypecaller_vcf ) - haplotypecaller_vcf_tbi = haplotypecaller_vcf + ch_haplotypecaller_vcf_tbi = ch_haplotypecaller_vcf .join(TABIX.out.tbi, by: [0], remainder: true) .join(TABIX.out.csi, by: [0], remainder: true) .map{meta, vcf, tbi, csi -> @@ -368,34 +368,34 @@ workflow RNAVAR { else [meta, vcf, csi] } - ch_versions = ch_versions.mix(TABIX.out.versions.first().ifEmpty(null)) - final_vcf = haplotypecaller_vcf + ch_versions = ch_versions.mix(TABIX.out.versions.first().ifEmpty(null)) + ch_final_vcf = ch_haplotypecaller_vcf // MODULE: VariantFiltration from GATK4 if (!params.skip_variantfiltration && !params.bam_csi_index ) { GATK4_VARIANTFILTRATION( - haplotypecaller_vcf_tbi, + ch_haplotypecaller_vcf_tbi, PREPARE_GENOME.out.fasta, PREPARE_GENOME.out.fai, PREPARE_GENOME.out.dict ) - filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf - final_vcf = filtered_vcf + ch_filtered_vcf = GATK4_VARIANTFILTRATION.out.vcf + ch_final_vcf = ch_filtered_vcf ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) } if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('snpeff') || params.annotate_tools.contains('vep'))) { ANNOTATE( - final_vcf, + ch_final_vcf, params.annotate_tools, - snpeff_db, - snpeff_cache, - vep_genome, - vep_species, - vep_cache_version, - vep_cache) + ch_snpeff_db, + ch_snpeff_cache, + ch_vep_genome, + ch_vep_species, + ch_vep_cache_version, + ch_vep_cache) // Gather QC reports ch_reports = ch_reports.mix(ANNOTATE.out.reports) From 253cbf8cbf944323618dc63fb72950403e59b15e Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 15:17:26 +0200 Subject: [PATCH 219/235] Clean up of blank lines --- subworkflows/local/prepare_genome.nf | 3 --- workflows/rnavar.nf | 5 ----- 2 files changed, 8 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 0802bd04..e4760b57 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -38,7 +38,6 @@ workflow PREPARE_GENOME { // // Uncompress GTF annotation file or create from GFF3 if required // - ch_gffread_version = Channel.empty() if (params.gtf) { if (params.gtf.endsWith('.gz')) { @@ -107,9 +106,7 @@ workflow PREPARE_GENOME { // // Uncompress STAR index or generate from scratch if required // - ch_star_index = Channel.empty() - if ('star' in prepare_tool_indices) { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 69a5ed4b..5eac4f37 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -170,7 +170,6 @@ workflow RNAVAR { // // MODULE: Run FastQC // - FASTQC ( ch_cat_fastq ) @@ -180,7 +179,6 @@ workflow RNAVAR { // // PREPARE THE INTERVAL LIST FROM GTF FILE // - ch_interval_list = Channel.empty() GATK4_BEDTOINTERVALLIST( ch_genome_bed, @@ -192,7 +190,6 @@ workflow RNAVAR { // // MODULE: IntervalListTools from GATK4 // - ch_interval_list_split = Channel.empty() if (!params.skip_intervallisttools) { GATK4_INTERVALLISTTOOLS( @@ -205,7 +202,6 @@ workflow RNAVAR { // // SUBWORKFLOW: Alignment with STAR // - ch_genome_bam = Channel.empty() ch_genome_bam_index = Channel.empty() ch_samtools_stats = Channel.empty() @@ -411,7 +407,6 @@ workflow RNAVAR { // // MODULE: MultiQC // - if (!params.skip_multiqc){ workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) From 4a9ad7b35a7281fa18f9bac042ee092bcc9c5e18 Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 15:19:33 +0200 Subject: [PATCH 220/235] Updated all test CI ymls to validate based on the testdata from repo --- tests/test_bamcsiindex.yml | 4 ---- tests/test_default.yml | 6 ------ 2 files changed, 10 deletions(-) diff --git a/tests/test_bamcsiindex.yml b/tests/test_bamcsiindex.yml index e1fd170e..06fb817f 100644 --- a/tests/test_bamcsiindex.yml +++ b/tests/test_bamcsiindex.yml @@ -6,9 +6,5 @@ - path: results/reports/multiqc_report.html - path: results/preprocessing/GM12878/GM12878.markdup.sorted.bam - path: results/preprocessing/GM12878/GM12878.markdup.sorted.bam.csi - - path: results/preprocessing/TEST123/TEST123.markdup.sorted.bam - - path: results/preprocessing/TEST123/TEST123.markdup.sorted.bam.csi - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.csi - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.csi diff --git a/tests/test_default.yml b/tests/test_default.yml index 79264af6..36cb35fa 100644 --- a/tests/test_default.yml +++ b/tests/test_default.yml @@ -11,9 +11,3 @@ - path: results/variant_calling/GM12878/GM12878.haplotypecaller.vcf.gz.tbi - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz - path: results/variant_calling/GM12878/GM12878.haplotypecaller.filtered.vcf.gz.tbi - - path: results/preprocessing/TEST123/TEST123.recal.bam - - path: results/preprocessing/TEST123/TEST123.recal.bam.bai - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.vcf.gz.tbi - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.filtered.vcf.gz - - path: results/variant_calling/TEST123/TEST123.haplotypecaller.filtered.vcf.gz.tbi From 203545eb195b9b34bdb1d9c10e2199b2253455df Mon Sep 17 00:00:00 2001 From: Praveen Date: Tue, 14 Jun 2022 15:34:19 +0200 Subject: [PATCH 221/235] Relabelled STAR params to be more clean and clear --- conf/modules.config | 6 +++--- nextflow.config | 14 +++++++------- nextflow_schema.json | 6 +++--- 3 files changed, 13 insertions(+), 13 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 5fca8a6a..61cf9ba1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -146,9 +146,9 @@ process { params.save_unaligned ? '--outReadsUnmapped Fastx' : '', params.read_length ? "--sjdbOverhang ${params.read_length - 1}" : '', params.star_twopass ? '--twopassMode Basic' : '', - params.star_limitBAMsortRAM > 0 ? "--limitBAMsortRAM ${params.star_limitBAMsortRAM}" : "", - params.star_outBAMsortingBinsN > 0 ? "--outBAMsortingBinsN ${params.star_outBAMsortingBinsN}" : "", - params.star_limitOutSJcollapsed > 0 ? "--limitOutSJcollapsed ${params.star_limitOutSJcollapsed}" : "" + params.star_max_memory_bamsort > 0 ? "--limitBAMsortRAM ${params.star_max_memory_bamsort}" : "", + params.star_bins_bamsort > 0 ? "--outBAMsortingBinsN ${params.star_bins_bamsort}" : "", + params.star_max_collapsed_junc > 0 ? "--limitOutSJcollapsed ${params.star_max_collapsed_junc}" : "" ].join(' ').trim() publishDir = [ [ diff --git a/nextflow.config b/nextflow.config index 007fded1..ee010d49 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,7 +12,7 @@ params { // Pipeline options // Mandatory option - input = null // sample sheet + input = null // sample sheet // Genome and reference options genome = 'GRCh38' @@ -25,12 +25,12 @@ params { read_length = 150 // Required for STAR to build index and align reads // Alignment - aligner = 'star' // Only STAR is currently supported. + aligner = 'star' // Only STAR is currently supported. star_twopass = true - star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR - star_limitBAMsortRAM = 0 - star_outBAMsortingBinsN = 50 - star_limitOutSJcollapsed = 1000000 + star_ignore_sjdbgtf = false // Ignore GTF file while creating index or alignment by STAR + star_max_memory_bamsort = 0 // STAR parameter limitBAMsortRAM to specify maximum RAM for sorting BAM + star_bins_bamsort = 50 // STAR parameter outBAMsortingBinsN to specify number of bins for sorting BAM + star_max_collapsed_junc = 1000000 // STAR parameter limitOutSJcollapsed to specify max number of collapsed junctions seq_center = null seq_platform = 'illumina' // Required for preparing for BAM headers for GATK to work bam_csi_index = false @@ -44,7 +44,7 @@ params { no_intervals = false // Variant annotation - annotate_tools = null // List of annotation tools to run - snpeff or vep or merge + annotate_tools = null // List of annotation tools to run - snpeff or vep or merge annotation_cache = false // Annotation cache disabled cadd_cache = null // CADD cache disabled cadd_indels = null // No CADD InDels file diff --git a/nextflow_schema.json b/nextflow_schema.json index 57a4f0e7..b8bfe2a1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -186,19 +186,19 @@ "description": "Do not use GTF file during STAR index buidling step", "help_text": "Do not use parameter --sjdbGTFfile during the STAR genomeGenerate process." }, - "star_limitBAMsortRAM": { + "star_max_memory_bamsort": { "type": "integer", "default": 0, "description": "Option to limit RAM when sorting BAM file. Value to be specified in bytes. If 0, will be set to the genome index size.", "help_text": "This parameter specifies the maximum available RAM (bytes) for sorting BAM during STAR alignment." }, - "star_outBAMsortingBinsN": { + "star_bins_bamsort": { "type": "integer", "default": 50, "description": "Specifies the number of genome bins for coordinate-sorting", "help_text": "This parameter specifies the number of bins to be used for coordinate sorting during STAR alignment step." }, - "star_limitOutSJcollapsed": { + "star_max_collapsed_junc": { "type": "integer", "default": 1000000, "description": "Specifies the maximum number of collapsed junctions" From 21ebd325e5658987cc8d67d2c9dc059eae7e4c76 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 15 Jun 2022 15:54:37 +0200 Subject: [PATCH 222/235] Edit: multiqc_config.yml to have the tools and their order in the report. --- assets/multiqc_config.yml | 53 +++++++++++++++++++++++++++++++++++++++ 1 file changed, 53 insertions(+) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index f3b0ac1d..60358731 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -9,3 +9,56 @@ report_section_order: order: -1001 export_plots: true + +# Run only these modules +run_modules: + - custom_content + - fastqc + - star + - samtools + - picard + - gatk + - snpeff + - vep + +# Order of modules +module_order: + - fastqc: + name: "FastQC (raw)" + path_filters: + - "*_val_*.zip" + - star: + name: "Read Alignment (STAR)" + - samtools: + name: "Samtools Flagstat" + - picard: + name: "GATK4 MarkDuplicates" + info: "Metrics generated either by GATK4 MarkDuplicates" + - qualimap: + name: "Qualimap" + - gatk: + name: "GATK4 BQSR" + - snpeff: + name: "SNPeff" + - vep: + name: "VEP" + +extra_fn_clean_exts: + - "_val" + +# Don't show % Dups in the General Stats table (we have this from Picard) +table_columns_visible: + fastqc: + percent_duplicates: False + +sp: + samtools/stats: + fn: "*.aligned.bam.stats" + samtools/flagstat: + fn: "*.aligned.bam.flagstat" + picard/markdups: + fn: "*.markdup.sorted.metrics" + snpeff: + contents: "SnpEff_version" + max_filesize: 5000000 + From 1b72d4ebde20193ae9d884f3e2189acae43efc32 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 15 Jun 2022 16:20:09 +0200 Subject: [PATCH 223/235] Added comments --- workflows/rnavar.nf | 46 ++++++++++++++++++++++++++++++++++----------- 1 file changed, 35 insertions(+), 11 deletions(-) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index 5eac4f37..34688263 100644 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -168,7 +168,7 @@ workflow RNAVAR { ch_versions = ch_versions.mix(CAT_FASTQ.out.versions.first().ifEmpty(null)) // - // MODULE: Run FastQC + // MODULE: Generate QC summary using FastQC // FASTQC ( ch_cat_fastq @@ -177,7 +177,7 @@ workflow RNAVAR { ch_versions = ch_versions.mix(FASTQC.out.versions.first()) // - // PREPARE THE INTERVAL LIST FROM GTF FILE + // MODULE: Prepare the interval list from the GTF file using GATK4 BedToIntervalList // ch_interval_list = Channel.empty() GATK4_BEDTOINTERVALLIST( @@ -188,7 +188,7 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_BEDTOINTERVALLIST.out.versions.first().ifEmpty(null)) // - // MODULE: IntervalListTools from GATK4 + // MODULE: Scatter one interval-list into many interval-files using GATK4 IntervalListTools // ch_interval_list_split = Channel.empty() if (!params.skip_intervallisttools) { @@ -200,7 +200,7 @@ workflow RNAVAR { else ch_interval_list_split = ch_interval_list // - // SUBWORKFLOW: Alignment with STAR + // SUBWORKFLOW: Perform read alignment using STAR aligner // ch_genome_bam = Channel.empty() ch_genome_bam_index = Channel.empty() @@ -229,7 +229,9 @@ workflow RNAVAR { ch_reports = ch_reports.mix(ALIGN_STAR.out.log_final.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(ALIGN_STAR.out.versions.first().ifEmpty(null)) + // // SUBWORKFLOW: Mark duplicates with GATK4 + // MARKDUPLICATES ( ch_genome_bam ) @@ -240,8 +242,10 @@ workflow RNAVAR { ch_reports = ch_reports.mix(MARKDUPLICATES.out.metrics.collect{it[1]}.ifEmpty([])) ch_versions = ch_versions.mix(MARKDUPLICATES.out.versions.first().ifEmpty(null)) - // Subworkflow - SplitNCigarReads from GATK4 over the intervals + // + // SUBWORKFLOW: SplitNCigarReads from GATK4 over the intervals // Splits reads that contain Ns in their cigar string (e.g. spanning splicing events in RNAseq data). + // ch_splitncigar_bam_bai = Channel.empty() SPLITNCIGAR ( ch_genome_bam, @@ -253,12 +257,13 @@ workflow RNAVAR { ch_splitncigar_bam_bai = SPLITNCIGAR.out.bam_bai ch_versions = ch_versions.mix(SPLITNCIGAR.out.versions.first().ifEmpty(null)) + // + // MODULE: BaseRecalibrator from GATK4 + // Generates a recalibration table based on various co-variates + // ch_bam_variant_calling = Channel.empty() - if(!params.skip_baserecalibration) { - // MODULE: BaseRecalibrator from GATK4 ch_bqsr_table = Channel.empty() - // known_sites is made by grouping both the dbsnp and the known indels ressources // they can either or both be optional ch_known_sites = ch_dbsnp.concat(ch_known_indels).collect() @@ -283,7 +288,6 @@ workflow RNAVAR { ch_reports = ch_reports.mix(ch_bqsr_table.map{ meta, table -> table}) ch_versions = ch_versions.mix(GATK4_BASERECALIBRATOR.out.versions.first().ifEmpty(null)) - // MODULE: ApplyBaseRecalibrator from GATK4 ch_bam_applybqsr = ch_splitncigar_bam_bai.join(ch_bqsr_table, by: [0]) ch_bam_recalibrated_qc = Channel.empty() @@ -292,6 +296,10 @@ workflow RNAVAR { .map{ meta, bam, bai, table, interval -> [ meta, bam, bai, table, interval] }.set{ch_applybqsr_bam_bai_interval} + // + // MODULE: ApplyBaseRecalibrator from GATK4 + // Recalibrates the base qualities of the input reads based on the recalibration table produced by the GATK BaseRecalibrator tool. + // RECALIBRATE( params.skip_multiqc, ch_applybqsr_bam_bai_interval, @@ -310,7 +318,6 @@ workflow RNAVAR { ch_bam_variant_calling = ch_splitncigar_bam_bai } - // MODULE: HaplotypeCaller from GATK4 interval_flag = params.no_intervals // Run haplotyper even in the absence of dbSNP files if (!params.dbsnp){ @@ -319,7 +326,6 @@ workflow RNAVAR { } ch_haplotypecaller_vcf = Channel.empty() - ch_haplotypecaller_interval_bam = ch_bam_variant_calling.combine(ch_interval_list_split) .map{ meta, bam, bai, interval_list -> new_meta = meta.clone() @@ -328,6 +334,10 @@ workflow RNAVAR { [new_meta, bam, bai, interval_list] } + // + // MODULE: HaplotypeCaller from GATK4 + // Calls germline SNPs and indels via local re-assembly of haplotypes. + // GATK4_HAPLOTYPECALLER( ch_haplotypecaller_interval_bam, PREPARE_GENOME.out.fasta, @@ -345,6 +355,10 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLER.out.versions.first().ifEmpty(null)) + // + // MODULE: MergeVCFS from GATK4 + // Merge multiple VCF files into one VCF + // GATK4_MERGEVCFS( ch_haplotypecaller_raw, PREPARE_GENOME.out.dict @@ -352,6 +366,9 @@ workflow RNAVAR { ch_haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) + // + // MODULE: Index the VCF using TABIX + // TABIX( ch_haplotypecaller_vcf ) @@ -367,7 +384,10 @@ workflow RNAVAR { ch_versions = ch_versions.mix(TABIX.out.versions.first().ifEmpty(null)) ch_final_vcf = ch_haplotypecaller_vcf + // // MODULE: VariantFiltration from GATK4 + // Filter variant calls based on certain criteria + // if (!params.skip_variantfiltration && !params.bam_csi_index ) { GATK4_VARIANTFILTRATION( @@ -382,6 +402,9 @@ workflow RNAVAR { ch_versions = ch_versions.mix(GATK4_VARIANTFILTRATION.out.versions.first().ifEmpty(null)) } + // + // SUBWORKFLOW: Annotate variants using snpEff and Ensembl VEP if enabled. + // if((!params.skip_variantannotation) && (params.annotate_tools) && (params.annotate_tools.contains('merge') || params.annotate_tools.contains('snpeff') || params.annotate_tools.contains('vep'))) { ANNOTATE( ch_final_vcf, @@ -406,6 +429,7 @@ workflow RNAVAR { // // MODULE: MultiQC + // Present summary of reads, alignment, duplicates, BSQR stats for all samples as well as workflow summary/parameters as single report // if (!params.skip_multiqc){ workflow_summary = WorkflowRnavar.paramsSummaryMultiqc(workflow, summary_params) From 7381dd31cf8c6a7502c77f4b3d999d68531f3018 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 15 Jun 2022 16:31:10 +0200 Subject: [PATCH 224/235] Fix: prettier --- assets/multiqc_config.yml | 1 - 1 file changed, 1 deletion(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 60358731..e1cb0b2b 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -61,4 +61,3 @@ sp: snpeff: contents: "SnpEff_version" max_filesize: 5000000 - From 1ea28550e533aa9eae88a950c8603cecee6b0422 Mon Sep 17 00:00:00 2001 From: Praveen Date: Wed, 15 Jun 2022 16:34:31 +0200 Subject: [PATCH 225/235] Update: comments --- bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 047b3fce..e9a7a178 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -115,7 +115,7 @@ def validate_unique_samples(self): Assert that the combination of sample name and FASTQ filename is unique. In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ file(s). + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. """ assert len(self._seen) == len(self.modified), "The pair of sample name and FASTQ must be unique." From 76515814c980eecaa7f39ecb43df808cdcdef77f Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Thu, 16 Jun 2022 15:09:01 +0200 Subject: [PATCH 226/235] Added the release date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ba683a54..ee641ef9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## [1.0.0] nfcore/rnavar - 2022/05/24 +## [1.0.0] nfcore/rnavar - 2022/06/20 First production release of the pipeline with latest software versions. From 1c4e0c1656255b69d5814baf044121cf4667345c Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 20 Jun 2022 11:51:33 +0200 Subject: [PATCH 227/235] Bump version to v1.1.0dev --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index ee010d49..8a37c30f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -236,7 +236,7 @@ manifest { description = 'GATK4 RNA variant calling pipeline' mainScript = 'main.nf' nextflowVersion = '>=21.10.3' - version = '1.0.0' + version = '1.1.0dev' } // Load modules.config for DSL2 module specific options From b69247fb1760927e1fc736a7dec66bf680b2c1ab Mon Sep 17 00:00:00 2001 From: Praveen Date: Mon, 20 Jun 2022 11:54:52 +0200 Subject: [PATCH 228/235] Edited changelog.md --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ba683a54..ec26605f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,9 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## [1.1.0dev] nfcore/rnavar + +New version with additional features and bug fixes. ## [1.0.0] nfcore/rnavar - 2022/05/24 First production release of the pipeline with latest software versions. From e86c5b74d16056383a3a2f8e450331b69b8cee94 Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Thu, 23 Jun 2022 11:05:21 +0200 Subject: [PATCH 229/235] Added Zenodo DOI badge --- README.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index 090f92b6..3a764555 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) -[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![Cite with Zenodo]((https://zenodo.org/badge/DOI/10.5281/zenodo.6669637.svg)](https://doi.org/10.5281/zenodo.6669637) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?logo=anaconda)](https://docs.conda.io/en/latest/) @@ -101,6 +101,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations +If you use nf-core/rnavar for your analysis, please cite it using the following doi: [10.5281/zenodo.6669637](https://doi.org/10.5281/zenodo.6669637) + An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. You can cite the `nf-core` publication as follows: From 051ad2b8ca4f9b87427353158a18b1b67f735ae3 Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Thu, 23 Jun 2022 11:06:53 +0200 Subject: [PATCH 230/235] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 3a764555..0cbbbe7d 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) -[![Cite with Zenodo]((https://zenodo.org/badge/DOI/10.5281/zenodo.6669637.svg)](https://doi.org/10.5281/zenodo.6669637) +[![Cite with Zenodo](https://zenodo.org/badge/DOI/10.5281/zenodo.6669637.svg)](https://doi.org/10.5281/zenodo.6669637) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?logo=anaconda)](https://docs.conda.io/en/latest/) From 6c382039e9457deed1abdc84316172bd2129e19b Mon Sep 17 00:00:00 2001 From: Praveen Raj S <43108054+praveenraj2018@users.noreply.github.com> Date: Thu, 23 Jun 2022 11:15:04 +0200 Subject: [PATCH 231/235] Updated Zenodo DOI --- README.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index 090f92b6..0cbbbe7d 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,7 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rnavar/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rnavar/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnavar/actions?query=workflow%3A%22nf-core+linting%22) [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?logo=Amazon%20AWS)](https://nf-co.re/rnavar/results) -[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![Cite with Zenodo](https://zenodo.org/badge/DOI/10.5281/zenodo.6669637.svg)](https://doi.org/10.5281/zenodo.6669637) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?logo=anaconda)](https://docs.conda.io/en/latest/) @@ -101,6 +101,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citations +If you use nf-core/rnavar for your analysis, please cite it using the following doi: [10.5281/zenodo.6669637](https://doi.org/10.5281/zenodo.6669637) + An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. You can cite the `nf-core` publication as follows: From 4f31fe05586fcf5604fec08347763e6dbff8efb0 Mon Sep 17 00:00:00 2001 From: mcharles Date: Thu, 24 Nov 2022 14:50:12 +0100 Subject: [PATCH 232/235] First commit --- conf/modules.config | 26 ++++ modules.json | 6 + modules/local/gtf2bed.nf | 14 +- .../nf-core/modules/bedtools/merge/main.nf | 36 +++++ .../nf-core/modules/bedtools/merge/meta.yml | 39 +++++ modules/nf-core/modules/bedtools/sort/main.nf | 37 +++++ .../nf-core/modules/bedtools/sort/meta.yml | 46 ++++++ .../modules/gatk4/combinegvcfs/main.nf | 48 ++++++ .../modules/gatk4/combinegvcfs/meta.yml | 61 ++++++++ subworkflows/local/prepare_genome.nf | 24 ++- workflows/rnavar.nf | 138 +++++++++++++++--- 11 files changed, 448 insertions(+), 27 deletions(-) mode change 100644 => 100755 conf/modules.config mode change 100644 => 100755 modules.json mode change 100644 => 100755 modules/local/gtf2bed.nf create mode 100644 modules/nf-core/modules/bedtools/merge/main.nf create mode 100644 modules/nf-core/modules/bedtools/merge/meta.yml create mode 100644 modules/nf-core/modules/bedtools/sort/main.nf create mode 100644 modules/nf-core/modules/bedtools/sort/meta.yml create mode 100755 modules/nf-core/modules/gatk4/combinegvcfs/main.nf create mode 100755 modules/nf-core/modules/gatk4/combinegvcfs/meta.yml mode change 100644 => 100755 subworkflows/local/prepare_genome.nf mode change 100644 => 100755 workflows/rnavar.nf diff --git a/conf/modules.config b/conf/modules.config old mode 100644 new mode 100755 index 61cf9ba1..a9ff5d12 --- a/conf/modules.config +++ b/conf/modules.config @@ -93,6 +93,7 @@ process { ] } + withName: SAMTOOLS_FAIDX { publishDir = [ path: { "${params.outdir}/genome" }, @@ -316,6 +317,22 @@ process { ].join(' ').trim() publishDir = [ enabled: false ] } + + withName: GATK4_HAPLOTYPECALLERGVCF { + ext.args = [ + '--dont-use-soft-clipped-bases', + params.gatk_hc_call_conf ? "--standard-min-confidence-threshold-for-calling $params.gatk_hc_call_conf" : '', + params.bam_csi_index ? "--create-output-variant-index false" : "", "-ERC GVCF" + ].join(' ').trim() + ext.prefix = {"${meta.id}.haplotypecaller"} + publishDir = [ enabled: false ] + } + + withName: GATK4_INDEXFEATUREFILE { + publishDir = [ + enabled: false + ] + } withName: GATK4_MERGEVCFS { ext.prefix = {"${meta.id}.haplotypecaller"} @@ -326,6 +343,15 @@ process { pattern: "*.{vcf.gz}" ] } + + withName: GATK4_COMBINEGVCFS { + ext.prefix = {"${meta.id}.haplotypecaller"} + publishDir = [ + path: { "${params.outdir}/variant_calling/${meta.id}" }, + mode: params.publish_dir_mode, + pattern: "*.{vcf.gz}" + ] + } withName: TABIX_TABIX { ext.args = params.bam_csi_index ? '--csi' : '' diff --git a/modules.json b/modules.json old mode 100644 new mode 100755 index c35f0779..6962f631 --- a/modules.json +++ b/modules.json @@ -57,6 +57,12 @@ "multiqc": { "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" }, + "bedtools/merge": { + "git_sha": "4bb1d4e362a38642e877afe41aaf58ded9e56c86" + }, + "bedtools/sort": { + "git_sha": "4bb1d4e362a38642e877afe41aaf58ded9e56c86" + }, "samtools/faidx": { "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" }, diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf old mode 100644 new mode 100755 index 0c434dcb..73d8a271 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -5,11 +5,12 @@ process GTF2BED { conda (params.enable_conda ? "conda-forge::r-base=3.5.0" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/r-base:3.5.0' : - 'quay.io/biocontainers/r-base:3.5.0' }" + 'quay.io/biocontainers/r-base:3.5.0'}" input: path gtf - + val feature_type + output: path '*.bed' , emit: bed path "versions.yml", emit: versions @@ -18,15 +19,20 @@ process GTF2BED { task.ext.when == null || task.ext.when script: // This script is bundled with the pipeline, in nf-core/rnaseq/bin/ + def allowed_type = ["exon", "transcript", "gene"]; + if (feature_type){ + feature_type = allowed_type.contains(feature_type) ? feature_type : "exon" + } + """ Rscript --no-save -<<'RCODE' gtf = read.table("${gtf}", sep="\t") - gtf = subset(gtf, V3 == "exon") + gtf = subset(gtf, V3 == "${feature_type}") write.table(data.frame(chrom=gtf[,'V1'], start=gtf[,'V4'], end=gtf[,'V5']), "tmp.exome.bed", quote = F, sep="\t", col.names = F, row.names = F) RCODE awk '{print \$1 "\t" (\$2 - 1) "\t" \$3}' tmp.exome.bed > exome.bed - rm tmp.exome.bed + rm -rf tmp.exome.bed cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/modules/bedtools/merge/main.nf b/modules/nf-core/modules/bedtools/merge/main.nf new file mode 100644 index 00000000..06dad822 --- /dev/null +++ b/modules/nf-core/modules/bedtools/merge/main.nf @@ -0,0 +1,36 @@ +process BEDTOOLS_MERGE { + tag "$meta.id" + label 'process_single' + + conda (params.enable_conda ? "bioconda::bedtools=2.30.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : + 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" + + input: + tuple val(meta), path(bed) + + output: + tuple val(meta), path('*.bed'), emit: bed + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if ("$bed" == "${prefix}.bed") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + bedtools \\ + merge \\ + -i $bed \\ + $args \\ + > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/bedtools/merge/meta.yml b/modules/nf-core/modules/bedtools/merge/meta.yml new file mode 100644 index 00000000..76743679 --- /dev/null +++ b/modules/nf-core/modules/bedtools/merge/meta.yml @@ -0,0 +1,39 @@ +name: bedtools_merge +description: combines overlapping or “book-ended” features in an interval file into a single feature which spans all of the combined features. +keywords: + - bed + - merge +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/merge.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: Input BED file + pattern: "*.{bed}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: Overlapped bed file with combined features + pattern: "*.{bed}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" diff --git a/modules/nf-core/modules/bedtools/sort/main.nf b/modules/nf-core/modules/bedtools/sort/main.nf new file mode 100644 index 00000000..331c129a --- /dev/null +++ b/modules/nf-core/modules/bedtools/sort/main.nf @@ -0,0 +1,37 @@ +process BEDTOOLS_SORT { + tag "$meta.id" + label 'process_single' + + conda (params.enable_conda ? "bioconda::bedtools=2.30.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : + 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" + + input: + tuple val(meta), path(intervals) + val extension + + output: + tuple val(meta), path("*.${extension}"), emit: sorted + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if ("$intervals" == "${prefix}.${extension}") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + bedtools \\ + sort \\ + -i $intervals \\ + $args \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/bedtools/sort/meta.yml b/modules/nf-core/modules/bedtools/sort/meta.yml new file mode 100644 index 00000000..369e51ff --- /dev/null +++ b/modules/nf-core/modules/bedtools/sort/meta.yml @@ -0,0 +1,46 @@ +name: bedtools_sort +description: Sorts a feature file by chromosome and other criteria. +keywords: + - bed + - sort +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/sort.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intervals: + type: file + description: BED/BEDGRAPH + pattern: "*.{bed|bedGraph}" + + - extension: + type: string + description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + + - sorted: + type: file + description: Sorted output file + pattern: "*.${extension}" + + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" + - "@chris-cheshire" diff --git a/modules/nf-core/modules/gatk4/combinegvcfs/main.nf b/modules/nf-core/modules/gatk4/combinegvcfs/main.nf new file mode 100755 index 00000000..488666b3 --- /dev/null +++ b/modules/nf-core/modules/gatk4/combinegvcfs/main.nf @@ -0,0 +1,48 @@ +process GATK4_COMBINEGVCFS { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::gatk4=4.2.6.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gatk4:4.2.6.1--hdfd78af_0': + 'quay.io/biocontainers/gatk4:4.2.6.1--hdfd78af_0' }" + + input: + tuple val(meta), path(vcf) + tuple val(meta), path(vcf_idx) + path fasta + path fai + path dict + + output: + tuple val(meta), path("*.combined.g.vcf.gz"), emit: combined_gvcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def input_list = vcf.collect{"--variant $it"}.join(' ') + + def avail_mem = 3 + if (!task.memory) { + log.info '[GATK COMBINEGVCFS] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = task.memory.giga + } + """ + gatk --java-options "-Xmx${avail_mem}g" CombineGVCFs \\ + $input_list \\ + --output ${prefix}.combined.g.vcf.gz \\ + --reference ${fasta} \\ + --tmp-dir . \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/gatk4/combinegvcfs/meta.yml b/modules/nf-core/modules/gatk4/combinegvcfs/meta.yml new file mode 100755 index 00000000..9330e084 --- /dev/null +++ b/modules/nf-core/modules/gatk4/combinegvcfs/meta.yml @@ -0,0 +1,61 @@ +name: gatk4_combinegvcfs +description: Combine per-sample gVCF files produced by HaplotypeCaller into a multi-sample gVCF file +keywords: + - gvcf + - gatk4 + - vcf + - combinegvcfs + - Short_Variant_Discovery +tools: + - gatk4: + description: + Genome Analysis Toolkit (GATK4). Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools + with a primary focus on variant discovery and genotyping. Its powerful processing engine + and high-performance computing features make it capable of taking on projects of any size. + homepage: https://gatk.broadinstitute.org/hc/en-us + documentation: https://gatk.broadinstitute.org/hc/en-us/articles/360037593911-CombineGVCFs + tool_dev_url: https://github.com/broadinstitute/gatk + doi: 10.1158/1538-7445.AM2017-3590 + licence: ["Apache-2.0"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - vcf: + type: file + description: Compressed VCF files + pattern: "*.vcf.gz" + - vcf_idx: + type: file + description: VCF Index file + pattern: "*.vcf.gz.idx" + - fasta: + type: file + description: The reference fasta file + pattern: "*.fasta" + - fai: + type: file + description: FASTA index file + pattern: "*.fasta.fai" + - dict: + type: file + description: FASTA dictionary file + pattern: "*.dict" +output: + - gvcf: + type: file + description: Compressed Combined GVCF file + pattern: "*.combined.g.vcf.gz" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@sateeshperi" + - "@mjcipriano" + - "@hseabolt" + - "@maxulysse" diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf old mode 100644 new mode 100755 index e4760b57..8768a515 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -4,7 +4,9 @@ include { GATK4_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/modules/gatk4/createsequencedictionary/main' //addParams(options: params.genome_options) include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' //addParams(options: params.gffread_options) -include { GTF2BED } from '../../modules/local/gtf2bed' //addParams(options: params.genome_options) +include { GTF2BED } from '../../modules/local/gtf2bed' +include { BEDTOOLS_SORT } from '../../modules/nf-core/modules/bedtools/sort/main' +include { BEDTOOLS_MERGE } from '../../modules/nf-core/modules/bedtools/merge/main' include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) include { GUNZIP as GUNZIP_GENE_BED } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/modules/gunzip/main' //addParams(options: params.genome_options) @@ -17,6 +19,7 @@ include { UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/module workflow PREPARE_GENOME { take: prepare_tool_indices + feature_type main: @@ -83,9 +86,24 @@ workflow PREPARE_GENOME { ch_gene_bed = Channel.fromPath(params.exon_bed).collect() } } else { - ch_exon_bed = GTF2BED ( ch_gtf ).bed.collect() + ch_exon_bed = GTF2BED ( ch_gtf , feature_type).bed.collect() ch_versions = ch_versions.mix(GTF2BED.out.versions) } + + ch_exon_bed.map{ it -> [[id:'exome'], it] } + + // Bedtools sort + ch_bedtools_sort = BEDTOOLS_SORT(ch_exon_bed, 'sorted').sorted.collect() + ch_versions = ch_versions.mix(BEDTOOLS_SORT.out.versions) + + + // Bedtools merge + //ch_test_meta=BEDTOOLS_SORT.out[0] + //ch_test_path=BEDTOOLS_SORT.out[1] + //ch_bedtools_merge = BEDTOOLS_MERGE(BEDTOOLS_SORT.out.sorted.collect()) + ch_bedtools_merge = BEDTOOLS_MERGE(ch_bedtools_sort) + ch_versions = ch_versions.mix(BEDTOOLS_MERGE.out.versions) + // Index the genome fasta ch_fasta_fai = Channel.empty() @@ -141,6 +159,8 @@ workflow PREPARE_GENOME { dict = ch_fasta_dict // path: genome.fasta.dict gtf = ch_gtf // path: genome.gtf exon_bed = ch_exon_bed // path: exon.bed + bedtools_sort = ch_bedtools_sort // path: sort.bed + bedtools_merge = ch_bedtools_merge // path: merge.bed star_index = ch_star_index // path: star/index/ versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf old mode 100644 new mode 100755 index 34688263..c434eefe --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -57,19 +57,30 @@ include { ANNOTATE } from '../subworkflows/local/annotate' ======================================================================================== */ -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' -include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' -include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' -include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' -include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' -include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/modules/gatk4/haplotypecaller/main' -include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' -include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' -include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' -include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' -include { TABIX_TABIX as TABIX } from '../modules/nf-core/modules/tabix/tabix/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/modules/fastqc/main' +include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' +include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' +include { GATK4_BASERECALIBRATOR } from '../modules/nf-core/modules/gatk4/baserecalibrator/main' +include { GATK4_BEDTOINTERVALLIST } from '../modules/nf-core/modules/gatk4/bedtointervallist/main' +include { GATK4_INTERVALLISTTOOLS } from '../modules/nf-core/modules/gatk4/intervallisttools/main' +include { GATK4_HAPLOTYPECALLER } from '../modules/nf-core/modules/gatk4/haplotypecaller/main' +include { GATK4_HAPLOTYPECALLER as GATK4_HAPLOTYPECALLERGVCF } from '../modules/nf-core/modules/gatk4/haplotypecaller/main' +include { GATK4_MERGEVCFS } from '../modules/nf-core/modules/gatk4/mergevcfs/main' +include { GATK4_COMBINEGVCFS } from '../modules/nf-core/modules/gatk4/combinegvcfs/main' +include { GATK4_INDEXFEATUREFILE } from '../modules/nf-core/modules/gatk4/indexfeaturefile/main' +include { GATK4_VARIANTFILTRATION } from '../modules/nf-core/modules/gatk4/variantfiltration/main' +include { SAMTOOLS_INDEX } from '../modules/nf-core/modules/samtools/index/main' +include { TABIX_TABIX as TABIX } from '../modules/nf-core/modules/tabix/tabix/main' +include { TABIX_TABIX as TABIXGVCF } from '../modules/nf-core/modules/tabix/tabix/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' + +/* +======================================================================================== + IMPORT LOCAL MODULES +======================================================================================== +*/ + +include { GTF2BED } from '../modules/local/gtf2bed' /* ======================================================================================== @@ -99,7 +110,7 @@ ch_dbsnp_tbi = params.dbsnp_tbi ? Channel.fromPath(params.dbs ch_known_indels = params.known_indels ? Channel.fromPath(params.known_indels).collect() : Channel.empty() ch_known_indels_tbi = params.known_indels_tbi ? Channel.fromPath(params.known_indels_tbi).collect() : Channel.empty() -// Initialize varaint annotation associated channels +// Initialize variant annotation associated channels ch_snpeff_db = params.snpeff_db ?: Channel.empty() ch_vep_cache_version = params.vep_cache_version ?: Channel.empty() ch_vep_genome = params.vep_genome ?: Channel.empty() @@ -110,6 +121,13 @@ ch_vep_cache = params.vep_cache ? Channel.fromPath(params. // MultiQC reporting def multiqc_report = [] +// GTF2bed parameters +def feature_type = params.feature_type ? params.feature_type : "exon" + + +// generate_gvcf parameters +def generate_gvcf = params.generate_gvcf ? params.generate_gvcf : false + /* ======================================================================================== RUN MAIN WORKFLOW RNAVAR @@ -127,7 +145,8 @@ workflow RNAVAR { // SUBWORKFLOW: Uncompress and prepare reference genome files // PREPARE_GENOME ( - prepareToolIndices + prepareToolIndices, + feature_type ) ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.exon_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) @@ -338,6 +357,7 @@ workflow RNAVAR { // MODULE: HaplotypeCaller from GATK4 // Calls germline SNPs and indels via local re-assembly of haplotypes. // + GATK4_HAPLOTYPECALLER( ch_haplotypecaller_interval_bam, PREPARE_GENOME.out.fasta, @@ -346,15 +366,16 @@ workflow RNAVAR { ch_dbsnp, ch_dbsnp_tbi ) - + + ch_haplotypecaller_raw = GATK4_HAPLOTYPECALLER.out.vcf - .map{ meta, vcf -> - meta.id = meta.sample - [meta, vcf]} - .groupTuple() + .map{ meta, vcf -> + meta.id = meta.sample + [meta, vcf]} + .groupTuple() ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLER.out.versions.first().ifEmpty(null)) - + // // MODULE: MergeVCFS from GATK4 // Merge multiple VCF files into one VCF @@ -366,6 +387,81 @@ workflow RNAVAR { ch_haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) + if (generate_gvcf){ + GATK4_HAPLOTYPECALLERGVCF( + ch_haplotypecaller_interval_bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict, + ch_dbsnp, + ch_dbsnp_tbi + ) + + ch_haplotypecallergvcf_raw = GATK4_HAPLOTYPECALLERGVCF.out.vcf + .map{ meta, vcf -> + meta.id = meta.sample + [meta, vcf]} + .groupTuple() + + ch_versions = ch_versions.mix(GATK4_HAPLOTYPECALLERGVCF.out.versions.first().ifEmpty(null)) + // + // MODULE: IndexFeatureFile from GATK4 + // Index the gVCF files + // + GATK4_INDEXFEATUREFILE( + GATK4_HAPLOTYPECALLERGVCF.out.vcf + ) + + ch_haplotypecallergvcf_raw_index = GATK4_INDEXFEATUREFILE.out.index + .map{ meta, idx -> + meta.id = meta.sample + [meta, idx]} + .groupTuple() + + ch_versions = ch_versions.mix(GATK4_INDEXFEATUREFILE.out.versions.first().ifEmpty(null)) + + // + // MODULE: CombineGVCFS from GATK4 + // Merge multiple GVCF files into one GVCF + // + + //ch_haplotypecallergvcf_raw_tbi = ch_haplotypecallergvcf_raw + // .join(ch_haplotypecallergvcf_raw_index, by: [0], remainder: true) + // .map{meta, vcf, tbi -> + // [meta, vcf, tbi] + // } + + + + GATK4_COMBINEGVCFS( + ch_haplotypecallergvcf_raw, + ch_haplotypecallergvcf_raw_index, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.dict + ) + ch_haplotypecaller_gvcf = GATK4_COMBINEGVCFS.out.combined_gvcf + ch_versions = ch_versions.mix(GATK4_COMBINEGVCFS.out.versions.first().ifEmpty(null)) + + // + // MODULE: Index the VCF using TABIX + // + TABIXGVCF( + ch_haplotypecaller_gvcf + ) + + ch_haplotypecaller_gvcf_tbi = ch_haplotypecaller_gvcf + .join(TABIXGVCF.out.tbi, by: [0], remainder: true) + .join(TABIXGVCF.out.csi, by: [0], remainder: true) + .map{meta, vcf, tbi, csi -> + if (tbi) [meta, vcf, tbi] + else [meta, vcf, csi] + } + + ch_versions = ch_versions.mix(TABIXGVCF.out.versions.first().ifEmpty(null)) + + } + // // MODULE: Index the VCF using TABIX // From c686eef9a1a6129c4315f2bf4a4a1bc64c1eaf77 Mon Sep 17 00:00:00 2001 From: mcharles Date: Wed, 30 Nov 2022 10:52:02 +0100 Subject: [PATCH 233/235] Add params to config and json files --- nextflow.config | 6 ++++-- nextflow_schema.json | 12 +++++++++++- subworkflows/local/prepare_genome.nf | 12 +++++------- workflows/rnavar.nf | 14 +++++--------- 4 files changed, 25 insertions(+), 19 deletions(-) diff --git a/nextflow.config b/nextflow.config index ee010d49..70b9ad23 100644 --- a/nextflow.config +++ b/nextflow.config @@ -20,6 +20,7 @@ params { igenomes_ignore = false save_reference = false save_merged_fastq = false + feature_type = 'exon' // Sequence read information read_length = 150 // Required for STAR to build index and align reads @@ -42,7 +43,7 @@ params { // Variant calling no_intervals = false - + // Variant annotation annotate_tools = null // List of annotation tools to run - snpeff or vep or merge annotation_cache = false // Annotation cache disabled @@ -67,8 +68,9 @@ params { // GATK intervallist parameters gatk_interval_scatter_count = 25 - //GATK haplotypecaller parameters + // GATK haplotypecaller parameters gatk_hc_call_conf = 20 + generate_gvcf = false //GATK variant filter parameters gatk_vf_window_size = 35 diff --git a/nextflow_schema.json b/nextflow_schema.json index b8bfe2a1..f71d87f3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -156,6 +156,11 @@ "type": "string", "fa_icon": "fas fa-tag", "description": "VEP cache version" + }, + "feature_type": { + "type": "string", + "description": "Type of feature to parse from annotation file", + "help_text": "This parameter value can be exon, transcript or gene. Default exon" } } }, @@ -260,7 +265,12 @@ "description": "The minimum phred-scaled confidence threshold at which variants should be called.", "help_text": "Specify the minimum phred-scaled confidence threshold at which variants should be called.", "hidden": false - } + }, + "generate_gvcf": { + "type": "boolean", + "description": "Enable generation of GVCFs by sample additionnaly to the VCFs.", + "help_text": "This parameter enables GATK HAPLOTYPECALLER to generate GVCFs. Default false." + }, } }, "variant_annotation": { diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 8768a515..74acecc8 100755 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -90,18 +90,16 @@ workflow PREPARE_GENOME { ch_versions = ch_versions.mix(GTF2BED.out.versions) } - ch_exon_bed.map{ it -> [[id:'exome'], it] } - + //ch_exon_bed.view() + //ch_exon_bed.map{ it -> [[id:'exome'], it] } + //ch_exon_bed.view() // Bedtools sort - ch_bedtools_sort = BEDTOOLS_SORT(ch_exon_bed, 'sorted').sorted.collect() + ch_bedtools_sort = BEDTOOLS_SORT(ch_exon_bed.map{ it -> [[id:'exome'], it] }, 'sorted').sorted.collect() ch_versions = ch_versions.mix(BEDTOOLS_SORT.out.versions) // Bedtools merge - //ch_test_meta=BEDTOOLS_SORT.out[0] - //ch_test_path=BEDTOOLS_SORT.out[1] - //ch_bedtools_merge = BEDTOOLS_MERGE(BEDTOOLS_SORT.out.sorted.collect()) - ch_bedtools_merge = BEDTOOLS_MERGE(ch_bedtools_sort) + ch_bedtools_merge = BEDTOOLS_MERGE(ch_bedtools_sort).bed ch_versions = ch_versions.mix(BEDTOOLS_MERGE.out.versions) diff --git a/workflows/rnavar.nf b/workflows/rnavar.nf index c434eefe..94d202dd 100755 --- a/workflows/rnavar.nf +++ b/workflows/rnavar.nf @@ -23,7 +23,8 @@ def checkPathParamList = [ params.known_indels_tbi, params.snpeff_cache, params.vep_cache, - params.star_index] + params.star_index, +] for (param in checkPathParamList) {if (param) file(param, checkIfExists: true)} @@ -121,12 +122,6 @@ ch_vep_cache = params.vep_cache ? Channel.fromPath(params. // MultiQC reporting def multiqc_report = [] -// GTF2bed parameters -def feature_type = params.feature_type ? params.feature_type : "exon" - - -// generate_gvcf parameters -def generate_gvcf = params.generate_gvcf ? params.generate_gvcf : false /* ======================================================================================== @@ -144,9 +139,10 @@ workflow RNAVAR { // // SUBWORKFLOW: Uncompress and prepare reference genome files // + PREPARE_GENOME ( prepareToolIndices, - feature_type + params.feature_type ) ch_genome_bed = Channel.from([id:'genome.bed']).combine(PREPARE_GENOME.out.exon_bed) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) @@ -387,7 +383,7 @@ workflow RNAVAR { ch_haplotypecaller_vcf = GATK4_MERGEVCFS.out.vcf ch_versions = ch_versions.mix(GATK4_MERGEVCFS.out.versions.first().ifEmpty(null)) - if (generate_gvcf){ + if (params.generate_gvcf){ GATK4_HAPLOTYPECALLERGVCF( ch_haplotypecaller_interval_bam, PREPARE_GENOME.out.fasta, From 88ade604c45334586f343426559bab6b4f3c7757 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 5 Dec 2022 09:27:15 +0000 Subject: [PATCH 234/235] [automated] Fix linting with Prettier --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index f71d87f3..e0ec0fc1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -270,7 +270,7 @@ "type": "boolean", "description": "Enable generation of GVCFs by sample additionnaly to the VCFs.", "help_text": "This parameter enables GATK HAPLOTYPECALLER to generate GVCFs. Default false." - }, + } } }, "variant_annotation": { From 86460530bff9b5ca9f078f4cf3666e0ed17f8da0 Mon Sep 17 00:00:00 2001 From: "Maxime U. Garcia" Date: Mon, 5 Dec 2022 10:33:56 +0100 Subject: [PATCH 235/235] Apply suggestions from code review remove trailing spaces --- conf/modules.config | 3 --- modules/local/gtf2bed.nf | 4 +--- 2 files changed, 1 insertion(+), 6 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a9ff5d12..f05b3322 100755 --- a/conf/modules.config +++ b/conf/modules.config @@ -317,7 +317,6 @@ process { ].join(' ').trim() publishDir = [ enabled: false ] } - withName: GATK4_HAPLOTYPECALLERGVCF { ext.args = [ '--dont-use-soft-clipped-bases', @@ -327,7 +326,6 @@ process { ext.prefix = {"${meta.id}.haplotypecaller"} publishDir = [ enabled: false ] } - withName: GATK4_INDEXFEATUREFILE { publishDir = [ enabled: false @@ -343,7 +341,6 @@ process { pattern: "*.{vcf.gz}" ] } - withName: GATK4_COMBINEGVCFS { ext.prefix = {"${meta.id}.haplotypecaller"} publishDir = [ diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index 73d8a271..a8baa452 100755 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -10,7 +10,6 @@ process GTF2BED { input: path gtf val feature_type - output: path '*.bed' , emit: bed path "versions.yml", emit: versions @@ -23,11 +22,10 @@ process GTF2BED { if (feature_type){ feature_type = allowed_type.contains(feature_type) ? feature_type : "exon" } - """ Rscript --no-save -<<'RCODE' gtf = read.table("${gtf}", sep="\t") - gtf = subset(gtf, V3 == "${feature_type}") + gtf = subset(gtf, V3 == "${feature_type}") write.table(data.frame(chrom=gtf[,'V1'], start=gtf[,'V4'], end=gtf[,'V5']), "tmp.exome.bed", quote = F, sep="\t", col.names = F, row.names = F) RCODE
    • \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "