You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
This is sort of a half-baked thought at this point, but it has occurred to me that much information is lost when selecting representative reference sequences to include in a reference package: consider the case when the observed biological diversity for a species consists of many identical or very closely related reference sequences, and a small number of more divergent sequences. It is likely that in this case we would select only one representative of the most prevalent variant to include in the reference package - and in this case pplacer has no way to know which of the reference sequences are more "authoritative" when performing classification. I wonder if there would be some way to represent the prevalence of individual reference sequences among all candidate reference sequences in the form of a weight, and whether the taxonomic assignment could be informed by these weights. Whether it would matter is of course another question... I could imagine that it might help mitigate classification artifacts caused by including "outlier" reference sequences in the reference package.
The text was updated successfully, but these errors were encountered:
This is sort of a half-baked thought at this point, but it has occurred to me that much information is lost when selecting representative reference sequences to include in a reference package: consider the case when the observed biological diversity for a species consists of many identical or very closely related reference sequences, and a small number of more divergent sequences. It is likely that in this case we would select only one representative of the most prevalent variant to include in the reference package - and in this case pplacer has no way to know which of the reference sequences are more "authoritative" when performing classification. I wonder if there would be some way to represent the prevalence of individual reference sequences among all candidate reference sequences in the form of a weight, and whether the taxonomic assignment could be informed by these weights. Whether it would matter is of course another question... I could imagine that it might help mitigate classification artifacts caused by including "outlier" reference sequences in the reference package.
The text was updated successfully, but these errors were encountered: