diff --git a/cmd/livekit-cli/room.go b/cmd/livekit-cli/room.go
index de76c455..d3d4e249 100644
--- a/cmd/livekit-cli/room.go
+++ b/cmd/livekit-cli/room.go
@@ -54,6 +54,10 @@ var (
 					Name:  "track-egress-file",
 					Usage: "AutoTrackEgress json file (see examples/auto-track-egress.json)",
 				},
+				&cli.StringFlag{
+					Name:  "agents-file",
+					Usage: "Agents configuration json file",
+				},
 				&cli.StringFlag{
 					Name:  "room-configuration",
 					Usage: "Name of the room configuration to associate with the created room",
@@ -285,6 +289,18 @@ func createRoom(c *cli.Context) error {
 		req.Egress.Tracks = trackEgress
 	}
 
+	if agentsFile := c.String("agents-file"); agentsFile != "" {
+		agent := &livekit.RoomAgent{}
+		b, err := os.ReadFile(agentsFile)
+		if err != nil {
+			return err
+		}
+		if err = protojson.Unmarshal(b, agent); err != nil {
+			return err
+		}
+		req.Agent = agent
+	}
+
 	if roomConfig := c.String("room-configuration"); roomConfig != "" {
 		req.ConfigName = roomConfig
 	}