Error in netVisual_bubble on LR pairs detected in only part groups

[XiangyuYe]

Thank you for developing such a powerful package tool. I am doing cell-cell communication comparison across three groups following this tutorial: .

However, when I tried to visualize some LR pairs up-/down-regulated in a certain group using netVisual_bubble(), it returned me error as "No significant signaling interactions are inferred based on the input!". I noted that it raised from lines 2154~2156 in script "R/analysis.R", and may be caused by the missing of my input LR pairs in one of the control group (for my case, these pairs were excluded due to 0 prob, i.e., "net <- subset(net, prob > 0)" at line 2063 in script "R/analysis.R").

Although I can fix this by modify corresponding code in function subsetCommunication_internal ), I am not sure whether it is reasonable to treat LR pairs specifically detected in only part groups as differentially regulated ones. I would appreciate it if you can give me some advice.

Best,
Xiangyu

[sqjin]

@XiangyuYe If LR pairs are specifically detected in only part groups, it can be certainly considered as differentially regulated ones.