Skip to content

Latest commit

 

History

History
279 lines (210 loc) · 11.5 KB

README.md

File metadata and controls

279 lines (210 loc) · 11.5 KB
Raw
title author date output
Build UCSC Track Hub from NCBI bigWigs
Jeffrey Long
10/15/2017
html_document
 
knitr::opts_chunk$set(echo = TRUE)

NCBI To UCSC

The Natioinal Center for Biotechnology Information, NCBI supplies data presented in scientific publications. These data support a wide range of experiments, including cancer research.

The UCSC Genome Browser is a widely used visualization tool for genomic data. Genomic functional features can be presented in many ways.

This effort acquires data from NCBI and uses tools developed by Jim Kent at UCSC to liftOver genomic data from human reference genome hg19 to hg38. The resulting bigWigs are then annotated to allow visualization in the UCSC Track Hubs. Within the UCSC Genome Browser, hypotheses can be generated and tested using a suite of tools in the UCSC Table Browser.

PLAN

  • Get data
  • Get toolchain
  • Convert [hg18].wig to [hg18].bigwig using wigToBigWig
  • Convert [hg18].bigwig to [hg18].bed using bigwigToBedGraph
  • Lift over the [hg18].bed to [hg38].bed
  • Convert the[hg38].bed back to bigwig using bedGraphToBigWig
  • Generated UCSC Track Hub files

DATA

The test data to be used is from Gozani lab, generated in 2011 and published by Kuo et. al. in "NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming". Two samples of chromatin immunoprecipitation next generation sequencing data (ChIP-Seq) will be used as examples. The control sample is labelled "input". The experimental data is "H3K36me2". Both data are for the biosample TKO2.

## Uncomment the next two lines to install wget on Mac OS.
# curl -fsSL https://raw.githubusercontent.com/Homebrew/install/master/install
# brew install wget --with-libressl
wget ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721530/suppl/GSM721530_TKO2_H3K36me2_rep1.wig.gz
wget ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM721nnn/GSM721532/suppl/GSM721532_TKO2_input.wig.gz

GET TOOLCHAIN

# Get toolchain
wget http://hgdownload.soe.ucsc.edu/admin/exe/macOSX.x86_64/wigToBigWig
wget http://hgdownload.soe.ucsc.edu/admin/exe/macOSX.x86_64/bigWigToBedGraph
wget http://hgdownload.soe.ucsc.edu/admin/exe/macOSX.x86_64/liftOver
wget http://hgdownload.soe.ucsc.edu/admin/exe/macOSX.x86_64/bedRemoveOverlap
wget http://hgdownload.soe.ucsc.edu/admin/exe/macOSX.x86_64/bedGraphToBigWig

# Adjust toolchain permissions
chmod 755 wigToBigWig
chmod 755 bigWigToBedGraph
chmod 755 liftOver
chmod 755 bedRemoveOverlap
chmod 755 bedGraphToBigWig

# Chain files specify mapping from one reference to another.
# This example uses hg18ToHg38.over.chain.
# The other two are commonly used.
wget --timestamping 'ftp://hgdownload.cse.ucsc.edu/goldenPath/hg19/liftOver/hg19ToHg38.over.chain.gz' -O hg19ToHg38.over.chain.gz
wget --timestamping 'ftp://hgdownload.cse.ucsc.edu/goldenPath/hg18/liftOver/hg18ToHg38.over.chain.gz' -O hg18ToHg38.over.chain.gz
wget --timestamping 'ftp://hgdownload.cse.ucsc.edu/goldenPath/hg38/liftOver/hg38ToHg19.over.chain.gz' -O hg38ToHg19.over.chain.gz
chmod 755 hg19ToHg38.over.chain.gz
chmod 755 hg18ToHg38.over.chain.gz
chmod 755 hg38ToHg19.over.chain.gz
gunzip hg19ToHg38.over.chain.gz
gunzip hg18ToHg38.over.chain.gz
gunzip hg38ToHg19.over.chain.gz
chmod 755 hg19ToHg38.over.chain
chmod 755 hg18ToHg38.over.chain
chmod 755 hg38ToHg19.over.chain

WIG to BIGWIG to BED, liftOver to hg38 to BIGWIG

Wig or wiggle files need to be converted to bigWig (bw) files before they can be converted to bed files. Further, bed files are required input format for liftOver, which maps the data to a new reference genome.

# Convert wigs to bigWigs
# The hg18.chrom.sizes file is retrieved from ucsc for 
#     conversion to bigWig
# Clip will issue warnings instead of killing process
#     when data does not map to chom.sizes.
./wigToBigWig GSM721532_TKO2_input.wig.gz http://hgdownload.cse.ucsc.edu/goldenPath/hg18/bigZips/hg18.chrom.sizes GSM721532_TKO2_input.wig.gz.bw -clip
./wigToBigWig GSM721530_TKO2_H3K36me2_rep1.wig.gz http://hgdownload.cse.ucsc.edu/goldenPath/hg18/bigZips/hg18.chrom.sizes GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw -clip

# Convert bigWig (bw) to bed (bedGraph)
# liftOver require bed formatted input files
./bigWigToBedGraph GSM721532_TKO2_input.wig.gz.bw GSM721532_TKO2_input.wig.gz.bw.bedGraph
./bigWigToBedGraph GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph


# liftOver from hg18 to hg38
./liftOver GSM721532_TKO2_input.wig.gz.bw.bedGraph hg18ToHg38.over.chain GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38 GSM721532_TKO2_input.wig.gz.bw.bedGraph.unMapped
./liftOver GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph hg18ToHg38.over.chain GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38 GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.unMapped

# Bed files must be sorted 
sort -k1,1 -k2,2n GGSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38 > GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38.sorted
sort -k1,1 -k2,2n GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38 > GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38.sorted

# Remove overlapping annotation from bed files
./bedRemoveOverlap GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38.sorted GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38.sorted.noOverlap
./bedRemoveOverlap GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38.sorted GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38.sorted.noOverlap

# Convert bed files to bigWig files
./bedGraphToBigWig GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38.sorted.noOverlap http://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes GSM721532_TKO2_input.wig.gz.bw.bedGraph.hg38.sorted.noOverlap.bw
./bedGraphToBigWig GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38.sorted.noOverlap http://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes GSM721530_TKO2_H3K36me2_rep1.wig.gz.bw.bedGraph.hg38.sorted.noOverlap.bw

GENERATE TRACK HUB FILES

TOOL DESCRIPTIONS

wigToBigWig v 4

Convert ascii format wig file (in fixedStep, variableStep or bedGraph format) to binary big wig format.

usage:

wigToBigWig in.wig chrom.sizes out.bw

Where in.wig is in one of the ascii wiggle formats, but not including track lines and chrom.sizes is a two-column file/URL: and out.bw is the output indexed big wig file.

If the assembly is hosted by UCSC, chrom.sizes can be a URL like http://hgdownload.cse.ucsc.edu/goldenPath//bigZips/.chrom.sizes or you may use the script fetchChromSizes to download the chrom.sizes file. If not hosted by UCSC, a chrom.sizes file can be generated by running twoBitInfo on the assembly .2bit file.

options:

  • blockSize=N - Number of items to bundle in r-tree. Default 256
  • itemsPerSlot=N - Number of data points bundled at lowest level. Default 1024
  • clip - If set just issue warning messages rather than dying if wig file contains items off end of chromosome.
  • unc - If set, do not use compression.
  • fixedSummaries - If set, use a predefined sequence of summary levels.
  • keepAllChromosomes - If set, store all chromosomes in b-tree.

bigWigToBedGraph

Convert from bigWig to bedGraph format.

usage:

bigWigToBedGraph in.bigWig out.bedGraph

options:

  • chrom=chr1 - if set restrict output to given chromosome
  • start=N - if set, restrict output to only that over start
  • end=N - if set, restict output to only that under end
  • udcDir=/dir/to/cache - place to put cache for remote bigBed/bigWigs

liftOver

Move annotations from one assembly to another

usage:

liftOver oldFile map.chain newFile unMapped

oldFile and newFile are in bed format by default, but can be in GFF and maybe eventually others with the appropriate flags below.

The map.chain file has the old genome as the target and the new genome as the query.

WARNING: liftOver was only designed to work between different assemblies of the same organism. It may not do what you want if you are lifting between different organisms. If there has been a rearrangement in one of the species, the size of the region being mapped may change dramatically after mapping.

options:

  • minMatch=0.N Minimum ratio of bases that must remap. Default 0.95
  • gff File is in gff/gtf format. Note that the gff lines are converted separately. It would be good to have a separate check after this that the lines that make up a gene model still make a plausible gene after liftOver
  • genePred - File is in genePred format
  • sample - File is in sample format
  • bedPlus=N - File is bed N+ format
  • positions - File is in browser "position" format
  • hasBin - File has bin value (used only with -bedPlus)
  • tab - Separate by tabs rather than space (used only with -bedPlus)
  • pslT - File is in psl format, map target side only
  • ends=N - Lift the first and last N bases of each record and combine the result. This is useful for lifting large regions like BAC end pairs.
  • minBlocks=0.N Minimum ratio of alignment blocks or exons that must map (default 1.00)
  • fudgeThick (bed 12 or 12+ only) If thickStart/thickEnd is not mapped, use the closest mapped base. Recommended if using * minBlocks.
  • multiple Allow multiple output regions
  • minChainT, -minChainQ Minimum chain size in target/query, when mapping to multiple output regions (default 0, 0)
  • minSizeT deprecated synonym for -minChainT (ENCODE compat.)
  • minSizeQ Min matching region size in query with -multiple.
  • chainTable Used with -multiple, format is db.tablename, to extend chains from net (preserves dups)
  • errorHelp Explain error messages

bedRemoveOverlap

Remove overlapping records from a (sorted) bed file.

Gets rid of the smaller of overlapping records.

usage:

bedRemoveOverlap in.bed out.bed

options:

-xxx=XXX

bedGraphToBigWig v 4

Convert a bedGraph file to bigWig format.

usage:

bedGraphToBigWig in.bedGraph chrom.sizes out.bw

where in.bedGraph is a four column file in the format:

  <chrom> <start> <end> <value>

and chrom.sizes is a two-column file/URL: and out.bw is the output indexed big wig file.

If the assembly is hosted by UCSC, chrom.sizes can be a URL like http://hgdownload.cse.ucsc.edu/goldenPath//bigZips/.chrom.sizes or you may use the script fetchChromSizes to download the chrom.sizes file. If not hosted by UCSC, a chrom.sizes file can be generated by running twoBitInfo on the assembly .2bit file.

The input bedGraph file must be sorted, use the unix sort command:

sort -k1,1 -k2,2n unsorted.bedGraph > sorted.bedGraph

options:

  • blockSize=N - Number of items to bundle in r-tree. Default 256
  • itemsPerSlot=N - Number of data points bundled at lowest level. Default 1024
  • unc - If set, do not use compression.

Fun Aside

Install Virtual Box to support GBiB.

References

bigWig and bigBed, Jim Kent 2010