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Orville Bennett Master's Thesis.out
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Orville Bennett Master's Thesis.out
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\BOOKMARK [0][-]{chapter.1}{Introduction}{}% 1
\BOOKMARK [1][-]{section.1.1}{Background}{chapter.1}% 2
\BOOKMARK [2][-]{subsection.1.1.1}{Calcium Signaling}{section.1.1}% 3
\BOOKMARK [2][-]{subsection.1.1.2}{Store-Operated Calcium Entry}{section.1.1}% 4
\BOOKMARK [1][-]{section.1.2}{STIM1}{chapter.1}% 5
\BOOKMARK [1][-]{section.1.3}{Orai3}{chapter.1}% 6
\BOOKMARK [1][-]{section.1.4}{Drosophila S2 cells}{chapter.1}% 7
\BOOKMARK [1][-]{section.1.5}{Metallothionein}{chapter.1}% 8
\BOOKMARK [1][-]{section.1.6}{Chemical reagents used}{chapter.1}% 9
\BOOKMARK [2][-]{subsection.1.6.1}{2-Aminoethoxyphenyl Borate}{section.1.6}% 10
\BOOKMARK [2][-]{subsection.1.6.2}{Cyclopiazonic Acid}{section.1.6}% 11
\BOOKMARK [2][-]{subsection.1.6.3}{Ethylene glycol tetraacetic acid}{section.1.6}% 12
\BOOKMARK [2][-]{subsection.1.6.4}{Fura-2}{section.1.6}% 13
\BOOKMARK [2][-]{subsection.1.6.5}{Probenecid}{section.1.6}% 14
\BOOKMARK [1][-]{section.1.7}{Specific Aims}{chapter.1}% 15
\BOOKMARK [2][-]{subsection.1.7.1}{Specific Aim \0431}{section.1.7}% 16
\BOOKMARK [2][-]{subsection.1.7.2}{Specific Aim \0432}{section.1.7}% 17
\BOOKMARK [2][-]{subsection.1.7.3}{Specific Aim \0433}{section.1.7}% 18
\BOOKMARK [1][-]{section.1.8}{Significance}{chapter.1}% 19
\BOOKMARK [0][-]{chapter.2}{Materials and Methods}{}% 20
\BOOKMARK [1][-]{section.2.1}{Materials}{chapter.2}% 21
\BOOKMARK [2][-]{subsection.2.1.1}{Restriction enzymes}{section.2.1}% 22
\BOOKMARK [2][-]{subsection.2.1.2}{Primers}{section.2.1}% 23
\BOOKMARK [2][-]{subsection.2.1.3}{Drosophila resources}{section.2.1}% 24
\BOOKMARK [1][-]{section.2.2}{Methods}{chapter.2}% 25
\BOOKMARK [2][-]{subsection.2.2.1}{Maintenance of cell lines}{section.2.2}% 26
\BOOKMARK [2][-]{subsection.2.2.2}{Creation of Drosophila expression constructs}{section.2.2}% 27
\BOOKMARK [2][-]{subsection.2.2.3}{Transfection of S2 cells}{section.2.2}% 28
\BOOKMARK [2][-]{subsection.2.2.4}{RT-PCR and cDNA synthesis}{section.2.2}% 29
\BOOKMARK [2][-]{subsection.2.2.5}{Ca2+ imaging experiments}{section.2.2}% 30
\BOOKMARK [0][-]{chapter.3}{Results}{}% 31
\BOOKMARK [1][-]{section.3.1}{Creating inducible vectors}{chapter.3}% 32
\BOOKMARK [1][-]{section.3.2}{Demonstrating inducible expression}{chapter.3}% 33
\BOOKMARK [1][-]{section.3.3}{Effective measurement of Ca2+ transients}{chapter.3}% 34
\BOOKMARK [0][-]{chapter.4}{Discussion}{}% 35
\BOOKMARK [1][-]{section.4.1}{Conclusion}{chapter.4}% 36
\BOOKMARK [0][-]{section.4.1}{Bibliography}{}% 37