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Add support for FusionCatcher #368

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iskandr opened this issue Sep 29, 2016 · 4 comments
Open

Add support for FusionCatcher #368

iskandr opened this issue Sep 29, 2016 · 4 comments

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@iskandr
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iskandr commented Sep 29, 2016

Identifies fusion proteins from RNAseq and also catches expression of viral sequences:

https://github.com/ndaniel/fusioncatcher

@hammer
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hammer commented Sep 29, 2016

May also want to consider https://sites.google.com/site/bioericscript as it's what UCSC's ProTECT uses for fusion calling in their neoepitope selection pipeline.

@arahuja
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arahuja commented Sep 30, 2016

I actually did this at some point: 4f3449d

I'm 80% certain this worked, but will re-test and submit a PR. The issue was that on many, many BAMs this required > 60 GB memory and would fail, which was really unfortunate.

@hammer
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hammer commented Sep 30, 2016

Grab a highmem type! https://cloud.google.com/compute/docs/machine-types

@arahuja
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arahuja commented Sep 30, 2016

Yea that is actually what spurred my move to gcloud originally, got a 128G machine to run all of our samples through.

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