Gor this Error: requested HMM banded DP mx of 5030.12 Mb > 2500.00 Mb limit. Increase limit with --mxsize or tau with --tau.

[itiago]

Hi
I'm trying to do annotation on assemble metagenome file, but got this error message.
The file has 78,734 contigs, and is 120Mb.
I've used PhyloSift to annotate the bins that I've determined, but wanted to have and overall phylogenetic analyses of the all community.

at the end I had the message error related to "Error: requested HMM banded DP mx of 5030.12 Mb > 2500.00 Mb limit. Increase limit with --mxsize or tau with --tau."

I know that there was a issue already with the same subject, but there was not posted a fix (at least that I noticed)

Thanks for any help.
Igor

ERROR MESSAGE I'VE GOT:

5
--------------------- WARNING ---------------------

,SG: seq doesn't validate with [0-9A-Za-z*-.=~/?], mismatch is

------------- EXCEPTION: Bio::Root::Exception -------------
CTTTCGACGACGCCAAGTTGCTGGGCA] which does not look healthyCGCTCAAGACCAAGATGAAAGACATGAACGCGGCCAGCATCGA
STACK: Error::throw
STACK: Bio::Root::Root::throw /diag/software/phylosift_v1.0.1/bin/../lib/Bio/Root/Root.pm:472
STACK: Bio::PrimarySeq::seq /diag/software/phylosift_v1.0.1/bin/../lib/Bio/PrimarySeq.pm:285
STACK: Bio::PrimarySeq::new /diag/software/phylosift_v1.0.1/bin/../lib/Bio/PrimarySeq.pm:239
STACK: Bio::LocatableSeq::new /diag/software/phylosift_v1.0.1/bin/../lib/Bio/LocatableSeq.pm:121
STACK: Phylosift::FastSearch::translate_frame /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/FastSearch.pm:73 3
STACK: Phylosift::FastSearch::write_candidates /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/FastSearch.pm:1 053
STACK: Phylosift::FastSearch::launch_searches /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/FastSearch.pm:31 9
STACK: Phylosift::FastSearch::run_search /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/FastSearch.pm:136
STACK: Phylosift::Phylosift::run_search /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/Phylosift.pm:418
STACK: Phylosift::Phylosift::run /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/Phylosift.pm:175
STACK: Phylosift::Command::all::execute /diag/software/phylosift_v1.0.1/bin/../lib/Phylosift/Command/all.pm:129
STACK: App::Cmd::execute_command /diag/software/phylosift_v1.0.1/bin/../lib/App/Cmd.pm:243
STACK: App::Cmd::run /diag/software/phylosift_v1.0.1/bin/../lib/App/Cmd.pm:171

STACK: /diag/software/phylosift_v1.0.1/bin/phylosift:7

Error: requested HMM banded DP mx of 5030.12 Mb > 2500.00 Mb limit.
Increase limit with --mxsize or tau with --tau.