chapter_05.md

layout	keywords	comments	image	title	subtitle	author	page_nav	prefix	contents
default		true	waves.jpg	Chapter V	'Water, Water Everywhere, Nor Any Drop to Drink': How Bacteria Adapt To Changes in Osmolarity	title title_url external_url description Summary false Mechanosensitive (MS) channels are transmembrane protein complexes which open and close in response to changes in membrane tension as a result of osmotic shock. Despite extensive biophysical characterization, the contribution of these channels to cell survival remains largely unknown. In this work, we use quantitative video microscopy to measure the abundance of a single species of MS channel in single cells followed by their survival after a large osmotic shock. We observe total death of the population with less than ~100 channels per cell and determine that approximately 500 - 700 channels are needed for 80% survival. The number of channels we find to confer nearly full survival is consistent with the counts of the number of channels in wild type cells in several earlier studies. These results prompt further studies to dissect the contribution of other channel species to survival.	prev next content url Chapter IV chapter_04 content url Chapter VI chapter_06	chapter_05	section_01_header section_02_abstract section_03_introduction section_04_results section_05_discussion section_06_methods

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