You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
When calling Molecular consensus reads using fgbio's CallMolecularConsensusReads, the soft clip disappears.
I went through CorrectUmis, GroupReadsByUmi, CallMolecularConsensusReads, alignment on a bam file mapped with raw fastq, and noticed that the soft clips disappeared on important singleton reads.
Given the presence of soft clips in the bam file resulting from GroupReadsByUmi, it seems that the issue is in CallMolecularConsensusReads.
I only gave CallMolecularConsensusReads the java option and the --min-reads=1 parameter.
There doesn't seem to be any parameter associated with CallMolecularConsensusReads (I could be wrong)
This is the same in 2.1.1, when collapsing between chromosomes became possible, and in 2.4.0, the current latest version.
Although it is a singleton read, it is an important read that represents a clinically meaningful fusion, so I don't want the soft clips to disappear.
May I request your opinion on this issue?
Thank you!
The text was updated successfully, but these errors were encountered:
hello
When calling Molecular consensus reads using fgbio's
CallMolecularConsensusReads
, the soft clip disappears.I went through
CorrectUmis
,GroupReadsByUmi
,CallMolecularConsensusReads
,alignment
on a bam file mapped with raw fastq, and noticed that the soft clips disappeared on important singleton reads.Given the presence of soft clips in the bam file resulting from
GroupReadsByUmi
, it seems that the issue is inCallMolecularConsensusReads
.I only gave
CallMolecularConsensusReads
the java option and the--min-reads=1
parameter.There doesn't seem to be any parameter associated with
CallMolecularConsensusReads
(I could be wrong)This is the same in
2.1.1
, when collapsing between chromosomes became possible, and in2.4.0
, the current latest version.Although it is a singleton read, it is an important read that represents a clinically meaningful fusion, so I don't want the soft clips to disappear.
May I request your opinion on this issue?
Thank you!
The text was updated successfully, but these errors were encountered: