Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Missing Pre-alignments with RefGenie in v0.10.0 #90

Open
CriticalSci opened this issue Apr 14, 2022 · 0 comments
Open

Missing Pre-alignments with RefGenie in v0.10.0 #90

CriticalSci opened this issue Apr 14, 2022 · 0 comments

Comments

@CriticalSci
Copy link

Hi @jpsmith5,

I recently installed PEPPRO on our cluster following the conda installation instructions. Everything appears to run fine except for the pre-alignment step. No matter what I define in the looper yaml input file, the pre-alignment to human_rDNA never happens and gets skipped despite having all the necessary RefGenie assets.

PEPPRO_log.md:

### Arguments passed to pipeline:
* `prealignment_index`:  `[]`
* `prealignment_names`:  `[]`
### Prealignments (04-13 18:55:48) elapsed: 251.0 _TIME_

You may use `--prealignment-index` to align to references before the genome alignment step. See http://peppro.databio.org/en/latest/ for documentation.

refgenie list lists:

genome assets
hg38 fasta, bowtie2_index, ensembl_gtf, ensembl_rb, refgene_anno, feat_annotation
human_rDNA fasta, bowtie2_index

I defined the prealignments in the yaml as per the examples in the repo:

  imply:
    - if:
        organism: ["human", "Homo sapiens", "Human", "Homo_sapiens"]
      then:
        genome: "hg38"
        prealignments: ["human_rDNA"] # also tried "human_rDNA"

The same input data/yaml/csv runs fine on the 0.9.11 PEPPRO install on a local machine though. I noticed there's been a lot of changes regarding RefGenie / prealignment code in peppro.py since 0.9.11 so perhaps you have a better idea of what could be going wrong with 0.10.0? Cheers!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant