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Genewise error #36

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lzyskyline opened this issue Jul 19, 2024 · 1 comment
Open

Genewise error #36

lzyskyline opened this issue Jul 19, 2024 · 1 comment

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@lzyskyline
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陈老师,您好,我在跑geta的第二步2.homolog_prediction时,出现以下报错:
failed to execute: /public/home/geta/bin/homolog_prediction --tmp_dir /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction --cpu 30 --identity 0.2 --evalue 1e-9 --homolog_coverage 0.3 --max_hits_num_per_match_region 10 --max_hit_num_per_single_species 2 --method exonerate,genewise,gth --threshod_ratio_of_intron_Supported_times 0.0 --genetic_code 1 --output_alignment_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_alignment.gff3 --output_raw_GFF3 /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.raw.gff3 /public/home/project/FX-geta/FX.tmp/homolog.fasta /public/home/project/FX-geta/FX.tmp/genome.fasta > /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.gff3 2> /public/home/project/FX-geta/FX.tmp/2.homolog_prediction/homolog_prediction.log
随后我单独运行了genewise,出现以下报错:
genewise /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.homolog.fasta /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/Chr01.49687_50382.start20354.AT2G01050_1.genome.fasta -codon /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/codon.table -trev -gff -genesf > /public/home/project/1_cooperate/FX-geta/FX.tmp/2.homolog_prediction/b.hitToGenePrediction/chunk0/genewise/Chr01.49687_50382.start20354.AT2G01050_1.gff
Warning Error
Unable to open gene.stat as gene stats file
Warning Error
Could not read gene statistics in gene.stat
Warning Error
Could not open BLOSUM62.bla as a filename for read Blast matrix
Warning Error
Could not read Comparison matrix file in BLOSUM62.bla
Fatal Error
Could not build objects!
请问,应该如何解决这个问题?谢谢

@dayuwww
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dayuwww commented Nov 17, 2024

你好,我也遇到了一样的报错,请问你解决了吗?

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