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Take E. coli K12 strain for primer position definitions > full-length gene > use RESCRIPt (which wraps VSEARCH) – use for trimming; can use to find position of alignment
Incorporate database of commonly used 16S primers
Consider entropy of first few bases? See alignment in first few segments?
The text was updated successfully, but these errors were encountered:
Suggested strategy, courtesy of Nick:
The text was updated successfully, but these errors were encountered: