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Automate identification of primers within samples #59

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lina-kim opened this issue Jun 2, 2023 · 0 comments
Open

Automate identification of primers within samples #59

lina-kim opened this issue Jun 2, 2023 · 0 comments
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enhancement New feature or request nice to have Stretch goal if time

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@lina-kim
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lina-kim commented Jun 2, 2023

Suggested strategy, courtesy of Nick:

  • Take E. coli K12 strain for primer position definitions > full-length gene > use RESCRIPt (which wraps VSEARCH) – use for trimming; can use to find position of alignment
  • Incorporate database of commonly used 16S primers
  • Consider entropy of first few bases? See alignment in first few segments?
@lina-kim lina-kim added enhancement New feature or request nice to have Stretch goal if time labels Jun 5, 2023
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Labels
enhancement New feature or request nice to have Stretch goal if time
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