diff --git a/.changeset/chatty-gifts-bathe.md b/.changeset/chatty-gifts-bathe.md
new file mode 100644
index 0000000000..cb7d99eba9
--- /dev/null
+++ b/.changeset/chatty-gifts-bathe.md
@@ -0,0 +1,5 @@
+---
+'@aws-amplify/backend': minor
+---
+
+Allow StackProps to be passed from defineBackend down to AmplifyStack for root Stack configuration
diff --git a/packages/backend/API.md b/packages/backend/API.md
index 1cb83ee09f..7efc2c19a5 100644
--- a/packages/backend/API.md
+++ b/packages/backend/API.md
@@ -31,6 +31,7 @@ import { ResourceProvider } from '@aws-amplify/plugin-types';
 import { SsmEnvironmentEntriesGenerator } from '@aws-amplify/plugin-types';
 import { SsmEnvironmentEntry } from '@aws-amplify/plugin-types';
 import { Stack } from 'aws-cdk-lib';
+import { StackProps } from 'aws-cdk-lib';
 
 export { a }
 
@@ -70,7 +71,12 @@ export { ConstructFactoryGetInstanceProps }
 export { defineAuth }
 
 // @public
-export const defineBackend: <T extends DefineBackendProps>(constructFactories: T) => Backend<T>;
+export const defineBackend: <T extends DefineBackendProps>(constructFactories: T, props?: DefineBackendOptions) => Backend<T>;
+
+// @public (undocumented)
+export type DefineBackendOptions = {
+    mainStackProps?: MainStackProps;
+};
 
 // @public (undocumented)
 export type DefineBackendProps = Record<string, ConstructFactory<ResourceProvider & Partial<ResourceAccessAcceptorFactory<never>>>> & {
@@ -89,6 +95,9 @@ export { GenerateContainerEntryProps }
 
 export { ImportPathVerifier }
 
+// @public
+export type MainStackProps = Pick<StackProps, 'env' | 'crossRegionReferences'>;
+
 export { ResourceProvider }
 
 // @public
diff --git a/packages/backend/src/backend.ts b/packages/backend/src/backend.ts
index a4e767ada2..055cde97d0 100644
--- a/packages/backend/src/backend.ts
+++ b/packages/backend/src/backend.ts
@@ -6,6 +6,7 @@ import {
 } from '@aws-amplify/plugin-types';
 import { Stack } from 'aws-cdk-lib';
 import { ClientConfig } from '@aws-amplify/client-config';
+import { MainStackProps } from './engine/amplify_stack.js';
 
 export type BackendBase = {
   createStack: (name: string) => Stack;
@@ -22,6 +23,10 @@ export type DefineBackendProps = Record<
   >
 > & { [K in keyof BackendBase]?: never };
 
+export type DefineBackendOptions = {
+  mainStackProps?: MainStackProps;
+};
+
 /**
  * Use `defineBackend` to create an instance of this type.
  * This object has the Amplify BackendBase methods on it for interacting with the backend.
diff --git a/packages/backend/src/backend_factory.ts b/packages/backend/src/backend_factory.ts
index 208a82ea17..ea66f1f82a 100644
--- a/packages/backend/src/backend_factory.ts
+++ b/packages/backend/src/backend_factory.ts
@@ -18,7 +18,11 @@ import { createDefaultStack } from './default_stack_factory.js';
 import { getBackendIdentifier } from './backend_identifier.js';
 import { platformOutputKey } from '@aws-amplify/backend-output-schemas';
 import { fileURLToPath } from 'node:url';
-import { Backend, DefineBackendProps } from './backend.js';
+import {
+  Backend,
+  DefineBackendOptions,
+  DefineBackendProps,
+} from './backend.js';
 import { AmplifyBranchLinkerConstruct } from './engine/branch-linker/branch_linker_construct.js';
 import {
   ClientConfig,
@@ -35,6 +39,9 @@ const rootStackTypeIdentifier = 'root';
 const DEFAULT_CLIENT_CONFIG_VERSION_FOR_BACKEND_ADD_OUTPUT =
   ClientConfigVersionOption.V1;
 
+// Stricter Omit for constructor overloading
+type ExplicitOmit<T, K extends keyof T> = Pick<T, Exclude<keyof T, K>>;
+
 /**
  * Factory that collects and instantiates all the Amplify backend constructs
  */
@@ -51,11 +58,37 @@ export class BackendFactory<
 
   private readonly stackResolver: StackResolver;
   private readonly customOutputsAccumulator: CustomOutputsAccumulator;
+
+  /**
+   * stack and props.mainStackProps are mutually exclusive
+   */
+  constructor(
+    constructFactories: T,
+    stack: Stack,
+    props?: ExplicitOmit<DefineBackendOptions, 'mainStackProps'>
+  );
+  /**
+   *
+   */
+  constructor(
+    constructFactories: T,
+    stack?: never,
+    props?: DefineBackendOptions
+  );
+
   /**
    * Initialize an Amplify backend with the given construct factories and in the given CDK App.
    * If no CDK App is specified a new one is created
    */
-  constructor(constructFactories: T, stack: Stack = createDefaultStack()) {
+  constructor(
+    constructFactories: T,
+    stack?: Stack,
+    props?: DefineBackendOptions
+  ) {
+    if (stack === undefined) {
+      stack = createDefaultStack(undefined, props?.mainStackProps);
+    }
+
     new AttributionMetadataStorage().storeAttributionMetadata(
       stack,
       rootStackTypeIdentifier,
@@ -150,9 +183,10 @@ export class BackendFactory<
  * @param constructFactories - list of backend factories such as those created by `defineAuth` or `defineData`
  */
 export const defineBackend = <T extends DefineBackendProps>(
-  constructFactories: T
+  constructFactories: T,
+  props?: DefineBackendOptions
 ): Backend<T> => {
-  const backend = new BackendFactory(constructFactories);
+  const backend = new BackendFactory(constructFactories, undefined, props);
   return {
     ...backend.resources,
     createStack: backend.createStack,
diff --git a/packages/backend/src/default_stack_factory.ts b/packages/backend/src/default_stack_factory.ts
index b084c6ce6d..26535c1e14 100644
--- a/packages/backend/src/default_stack_factory.ts
+++ b/packages/backend/src/default_stack_factory.ts
@@ -1,14 +1,21 @@
 import { App, Stack } from 'aws-cdk-lib';
 import { ProjectEnvironmentMainStackCreator } from './project_environment_main_stack_creator.js';
 import { getBackendIdentifier } from './backend_identifier.js';
+import { MainStackProps } from './engine/amplify_stack.js';
 
 /**
  * Creates a default CDK scope for the Amplify backend to use if no scope is provided to the constructor
  */
-export const createDefaultStack = (app = new App()): Stack => {
+export const createDefaultStack = (
+  app?: App,
+  props?: MainStackProps
+): Stack => {
+  if (app === undefined) {
+    app = new App();
+  }
   const mainStackCreator = new ProjectEnvironmentMainStackCreator(
     app,
     getBackendIdentifier(app)
   );
-  return mainStackCreator.getOrCreateMainStack();
+  return mainStackCreator.getOrCreateMainStack(props);
 };
diff --git a/packages/backend/src/engine/amplify_stack.ts b/packages/backend/src/engine/amplify_stack.ts
index 4c7475658b..e775f69c10 100644
--- a/packages/backend/src/engine/amplify_stack.ts
+++ b/packages/backend/src/engine/amplify_stack.ts
@@ -1,8 +1,31 @@
 import { AmplifyFault } from '@aws-amplify/platform-core';
-import { Aspects, CfnElement, IAspect, Stack } from 'aws-cdk-lib';
+import { Aspects, CfnElement, IAspect, Stack, StackProps } from 'aws-cdk-lib';
 import { Role } from 'aws-cdk-lib/aws-iam';
 import { Construct, IConstruct } from 'constructs';
 
+/**
+ * Props for root CDK Stack
+ * @see {@link https://docs.aws.amazon.com/cdk/api/v2/docs/aws-cdk-lib.StackProps.html | AWS documentation for aws-cdk-lib.StackProps}
+ *
+ * Props are picked to ensure explicit addition of new StackProps is required.
+ * Props incompatible with Amplify's intended Stack hierarchy, build or deployment processes should always be ommited:
+ * - stackName: Conflicts with dynamic resource naming.
+ * - synthesizer: Conflicts with managed deployments and resource references.
+ * - terminationProtection: Conflicts with sandbox/app delete.
+ * - permissionsBoundary: Conflicts with single root Stack ethos (i.e. Unable to create Role prior to `defineBackend`).
+ *
+ * Props are passed down from `defineBackend`:
+ * @example <caption>Set explicit region (e.g. for `new cloudfront.experimental.EdgeFunction`)</caption>
+ * ```
+ * defineBackend({}, {
+ *   env:
+ *     region: 'us-east-1' // Any valid AWS region
+ *   }
+ * })
+ * ```
+ */
+export type MainStackProps = Pick<StackProps, 'env' | 'crossRegionReferences'>;
+
 /**
  * Amplify-specific Stack implementation to handle cross-cutting concerns for all Amplify stacks
  */
@@ -10,8 +33,8 @@ export class AmplifyStack extends Stack {
   /**
    * Default constructor
    */
-  constructor(scope: Construct, id: string) {
-    super(scope, id);
+  constructor(scope: Construct, id: string, props?: MainStackProps) {
+    super(scope, id, props);
     Aspects.of(this).add(new CognitoRoleTrustPolicyValidator());
   }
   /**
diff --git a/packages/backend/src/index.ts b/packages/backend/src/index.ts
index 039b5de54c..0ece4012a9 100644
--- a/packages/backend/src/index.ts
+++ b/packages/backend/src/index.ts
@@ -1,3 +1,4 @@
+export { type MainStackProps } from './engine/amplify_stack.js';
 export { defineBackend } from './backend_factory.js';
 export * from './backend.js';
 export * from './secret.js';
diff --git a/packages/backend/src/project_environment_main_stack_creator.ts b/packages/backend/src/project_environment_main_stack_creator.ts
index fbadb59b57..08f3353067 100644
--- a/packages/backend/src/project_environment_main_stack_creator.ts
+++ b/packages/backend/src/project_environment_main_stack_creator.ts
@@ -1,7 +1,7 @@
 import { BackendIdentifier, MainStackCreator } from '@aws-amplify/plugin-types';
 import { Construct } from 'constructs';
 import { Stack, Tags } from 'aws-cdk-lib';
-import { AmplifyStack } from './engine/amplify_stack.js';
+import { AmplifyStack, MainStackProps } from './engine/amplify_stack.js';
 import { BackendIdentifierConversions } from '@aws-amplify/platform-core';
 
 /**
@@ -20,11 +20,12 @@ export class ProjectEnvironmentMainStackCreator implements MainStackCreator {
   /**
    * Get a stack for this environment in the provided CDK scope
    */
-  getOrCreateMainStack = (): Stack => {
+  getOrCreateMainStack = (props?: MainStackProps): Stack => {
     if (this.mainStack === undefined) {
       this.mainStack = new AmplifyStack(
         this.scope,
-        BackendIdentifierConversions.toStackName(this.backendId)
+        BackendIdentifierConversions.toStackName(this.backendId),
+        props
       );
     }