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real time rigid registration on confocal microscope acquisition to adjust lens position for time imaging

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CONFOCAL REGISTRATION PROJECT

Gofigure2 Team,
Megason Lab

This project is aimed at automatically moving the confocal microscope while imaging.
It uses a registration process between timepoints to determine global displacement of the sample.

This is an experimental project :
for now:

  • we use a translation transform in the registration process, which doesn’t consider the shape modification of the sample.
  • we only register 2D images in a representative area of the sample.

in the future, we may:

  • use a non rigid transform and evaluate displacement in the border of the images.
  • modify the optimizer
  • modify the metric
  • take into account the 3rd dimension
  • use a Kalman filter to predict displacements

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real time rigid registration on confocal microscope acquisition to adjust lens position for time imaging

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