diff --git a/bindings/python/python/opendal/__init__.pyi b/bindings/python/python/opendal/__init__.pyi
index 0ebadaea2ee3..64fedbbf09c6 100644
--- a/bindings/python/python/opendal/__init__.pyi
+++ b/bindings/python/python/opendal/__init__.pyi
@@ -86,6 +86,7 @@ class AsyncOperator:
 @final
 class File:
     def read(self, size: Optional[int] = None) -> bytes: ...
+    def readline(self, size: Optional[int] = None) -> bytes: ...
     def write(self, bs: bytes) -> None: ...
     def seek(self, pos: int, whence: int = 0) -> int: ...
     def tell(self) -> int: ...
diff --git a/bindings/python/src/file.rs b/bindings/python/src/file.rs
index be7a461893e3..b23c6d2903cd 100644
--- a/bindings/python/src/file.rs
+++ b/bindings/python/src/file.rs
@@ -18,6 +18,7 @@
 // Remove this `allow` after <https://github.com/rust-lang/rust-clippy/issues/12039> fixed.
 #![allow(clippy::unnecessary_fallible_conversions)]
 
+use std::io::BufRead;
 use std::io::Read;
 use std::io::Seek;
 use std::io::SeekFrom;
@@ -97,6 +98,51 @@ impl File {
         Buffer::new(buffer).into_bytes_ref(py)
     }
 
+    /// Read a single line from the file.
+    /// A newline character (`\n`) is left at the end of the string, and is only omitted on the last line of the file if the file doesn’t end in a newline.
+    /// If size is specified, at most size bytes will be read.
+    #[pyo3(signature = (size=None,))]
+    pub fn readline<'p>(
+        &'p mut self,
+        py: Python<'p>,
+        size: Option<usize>,
+    ) -> PyResult<Bound<PyAny>> {
+        let reader = match &mut self.0 {
+            FileState::Reader(r) => r,
+            FileState::Writer(_) => {
+                return Err(PyIOError::new_err(
+                    "I/O operation failed for reading on write only file.",
+                ));
+            }
+            FileState::Closed => {
+                return Err(PyIOError::new_err(
+                    "I/O operation failed for reading on closed file.",
+                ));
+            }
+        };
+
+        let buffer = match size {
+            None => {
+                let mut buffer = Vec::new();
+                reader
+                    .read_until(b'\n', &mut buffer)
+                    .map_err(|err| PyIOError::new_err(err.to_string()))?;
+                buffer
+            }
+            Some(size) => {
+                let mut bs = vec![0; size];
+                let mut reader = reader.take(size as u64);
+                let n = reader
+                    .read_until(b'\n', &mut bs)
+                    .map_err(|err| PyIOError::new_err(err.to_string()))?;
+                bs.truncate(n);
+                bs
+            }
+        };
+
+        Buffer::new(buffer).into_bytes_ref(py)
+    }
+
     /// Read bytes into a pre-allocated, writable buffer
     pub fn readinto(&mut self, buffer: PyBuffer<u8>) -> PyResult<usize> {
         let reader = match &mut self.0 {
diff --git a/bindings/python/tests/test_read.py b/bindings/python/tests/test_read.py
index ddbd5f08a9d4..73ccd04f7d5c 100644
--- a/bindings/python/tests/test_read.py
+++ b/bindings/python/tests/test_read.py
@@ -16,7 +16,8 @@
 # under the License.
 
 import os
-from random import randint
+import io
+from random import randint, choices
 from uuid import uuid4
 
 import pytest
@@ -73,6 +74,33 @@ def test_sync_reader(service_name, operator, async_operator):
     operator.delete(filename)
 
 
+@pytest.mark.need_capability("read", "write", "delete")
+def test_sync_reader_readline(service_name, operator, async_operator):
+    size = randint(1, 1024)
+    lines = randint(1, min(100, size))
+    filename = f"random_file_{str(uuid4())}"
+    content = bytearray(os.urandom(size))
+
+    for idx in choices(range(0, size), k=lines):
+        content[idx] = ord("\n")
+    operator.write(filename, content)
+
+    line_contents = io.BytesIO(content).readlines()
+    i = 0
+
+    with operator.open(filename, "rb") as reader:
+        assert reader.readable()
+        assert not reader.writable()
+        assert not reader.closed
+
+        while (read_content := reader.readline()) != b"":
+            assert read_content is not None
+            assert read_content == line_contents[i]
+            i += 1
+
+    operator.delete(filename)
+
+
 @pytest.mark.asyncio
 @pytest.mark.need_capability("read", "write", "delete")
 async def test_async_read(service_name, operator, async_operator):