-ref and -wgref arguments are required

[luzhouyangge]

Hi, I am using the non-docker version, installed with v1.3.3.

When run the text.py, error occurs:

1. Testing extraction of genome regions...

ERROR (1)! If the docker image without reference genome or non-docker version is used, -ref and -wgref arguments are required.

How to solve the error?

Thank you

[aakechin]

@luzhouyangge you need to use both parameters -ref and -wgref. Without them, the program doesn't know which reference genome you are going to use.

[luzhouyangge]

Please list the files in hg19. Thank you!

[luzhouyangge]

Always error

[aakechin]

@luzhouyangge you need to download and prepare GenBank files to use getGeneRegions.py script as it is described at https://github.com/aakechin/NGS-PrimerPlex#reference-genome
Also, it is described in details at Wiki pages: https://github.com/aakechin/NGS-PrimerPlex/wiki/NGS-PrimerPlex-installation-in-linux-as-a-standalone-tool
For example, in my hg19 directory there are the following GenBank files:
chr10.gb
chr11.gb
chr12.gb
chr13.gb
chr14.gb
chr15.gb
chr16.gb
chr17.gb
chr18.gb
chr19.gb
chr1.gb
chr20.gb
chr21.gb
chr22.gb
chr2.gb
chr3.gb
chr4.gb
chr5.gb
chr6.gb
chr7.gb
chr8.gb
chr9.gb
chrM.gb
chrX.gb
chrY.gb