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+---
+layout: default
+math: mathjax
+title: Metrics Bcftools counts
+nav_order: 5
+---
+
+Last update: 20241219
+
+# Metrics Bcftools counts
+
+This is the main source of basic variant counts when understanding the effects of a qualifying variant (QV) design.
+
+The script shown below runs on a set of gVCF directories (`INPUT_DIRS`) where incremental filtering has reduces the dataset in each sequential step. 
+A pyramid filtering plot is likely to use such count data. 
+
+**Example output**:
+
+* Number of samples: 180
+* Number of SNPs:    97296
+* Number of INDELs:  17945
+* Number of MNPs:    0
+* Number of others:  0
+* Number of sites:   112132
+
+Usage Example:
+
+```
+#!/bin/bash
+
+#SBATCH --array=0-24
+...
+...
+
+set -e
+echo "START AT $(date)"
+
+variables="/path/variables.sh"
+source ${variables}
+QV_MODEL="Design_QV_SNV_INDEL_v1"
+
+# Tools setup
+module load StdEnv/2023 gcc/12.3 bcftools/1.19
+
+# Input directories setup
+declare -a INPUT_DIRS=(
+	"${VQSR_DIR}"
+	"${GENOTYPE_REFINEMENT_DIR}"
+	"${PRE_ANNOTATION_DIR}"
+	"${PRE_ANNOTATION_MAF_DIR}"
+	"${ANNOTATION_DIR}"
+	"${ANNOTATION_GNOMAD_DIR}"
+)
+
+declare -a NUMBER
+for j in {1..22} X Y M; do NUMBER+=($j); done
+INDEX=${NUMBER[$SLURM_ARRAY_TASK_ID]}
+
+# Processing each directory
+for INPUT_DIR in "${INPUT_DIRS[@]}"; do
+
+	echo " "
+	echo "Working on directory: ${INPUT_DIR}"
+	
+	# Create an array of VCF files for the current chromosome
+	mapfile -t vcf_files < <(ls ${INPUT_DIR}/chr${INDEX}_*.vcf.gz)
+	
+	# Process each VCF file in the directory
+	for vcf in "${vcf_files[@]}"; do
+		# Output file path for count results
+		count_out="${QC_SUMMARY_STATS}/all_gvcf/${QV_MODEL}_$(basename "${vcf}" .vcf.gz)_qc.log"
+		
+		# Get basic variant counts
+		echo "Input: ${vcf}"
+		echo "Output: ${count_out}"
+		bcftools +counts "${vcf}" > "${count_out}"
+	done
+done
+
+echo "END AT $(date)"
+```