Negative

import cv2
import matplotlib.pyplot as plt

def main():
    path = "C:\\Users\\edkni\\Downloads\\image_processing_files\\Dataset\\"
    
    imgpath1 =  path + "4.2.06.tiff"
    img1 = cv2.imread(imgpath1, 1)
    
    img1 = cv2.cvtColor(img1,cv2.COLOR_BGR2RGB)
    img2 = cv2.cvtColor(img1,cv2.COLOR_BGR2GRAY)
    img3 = abs(255 - img1)
    img4 = abs(255 - img2)
    
    titles = ["Original image","Gray","Original Negative","Gray Negative"]
    images = [img1,img2,img3,img4]

 
    plt.subplot(2,2,1)
    plt.imshow(images[0])
    plt.title(titles[0])
    plt.xticks([])
    plt.yticks([])
       
    plt.subplot(2,2,2)
    plt.imshow(images[1],cmap = "gray")
    plt.title(titles[1])
    plt.xticks([])
    plt.yticks([])
    
    plt.subplot(2,2,3)
    plt.imshow(images[2])
    plt.xticks([])
    plt.yticks([])    
    plt.title(titles[2])

    plt.subplot(2,2,4)
    plt.imshow(images[3],cmap = "gray")
    plt.xticks([])
    plt.yticks([])
    plt.title(titles[3])
    plt.show() 

if __name__ == "__main__":
    main()