The read.FCS analysis reveals a discrepancy of approximately 100-fold.

[JAEYOONSUNG]

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The values obtained from Bio-Rad's statistics and the statistical analysis of FL1-Area using read.FCS show a difference of approximately 100-fold. Can you explain why such a phenomenon occurs?

To Reproduce
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Expected behavior
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flowCore::read.FCS("filenames.fcs",
transformation="linearized")

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sessionInfo():

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[gfinak]

Maybe you can include some code to actually reproduce the statistical analysis you are trying to do as well as an explanation of what this statistical analysis is supposed to do so that we might determine why it's different or if it's even comparing apples to apples, so to speak.