From c02cc7e90325e798ce7128f88d4d01dca4738af2 Mon Sep 17 00:00:00 2001 From: Chang Y Date: Mon, 20 May 2019 19:49:54 +0800 Subject: [PATCH] --ccs typo --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 324060b..605578e 100644 --- a/README.md +++ b/README.md @@ -91,7 +91,7 @@ Run on raw subread data: Run on CCS data: - lima --css movie.ccs.bam barcodes.fasta prefix.bam + lima --ccs movie.ccs.bam barcodes.fasta prefix.bam lima --ccs movie.consensusreadset.xml barcodes.barcodeset.xml prefix.consensusreadset.xml If you do not need to import the demultiplexed data into SMRT Link, it is advised @@ -967,7 +967,7 @@ The score lead measures how close the best barcode call is to the second best. Possible solutions without seeing your data: * Is that sample actually barcoded? * Are your barcode sequences genetically too close for SMRT sequencing? - Try CCS2 calling first and demultiplex with `--css`. + Try CCS2 calling first and demultiplex with `--ccs`. * Are the synthesized products clean and not degenerate? * Did the sequencing run perform optimally, is the accuracy in the expected range? * Did you run lima twice, first on the original and then on the already