From 9c74517adea51c31e66aed41be7303f0edd4964c Mon Sep 17 00:00:00 2001 From: Jessica Mattick <19544292+jmattick@users.noreply.github.com> Date: Tue, 7 Nov 2023 14:22:48 -0600 Subject: [PATCH] Update isoseq version 4.1.0 (#54) * Update isoseq version 4.1.0 * Fix formatting --------- Co-authored-by: Jessica Mattick --- docs/changelog.md | 6 +++++- docs/classification/pigeon-input.md | 5 ++++- docs/clustering/cli-workflow.md | 2 ++ 3 files changed, 11 insertions(+), 2 deletions(-) diff --git a/docs/changelog.md b/docs/changelog.md index 8c9ea2e..c8f202d 100644 --- a/docs/changelog.md +++ b/docs/changelog.md @@ -5,7 +5,11 @@ nav_order: 99 --- # Version changelog - * **4.0.0** + * **4.1.0** + * Update minimap2 to version 2.26 + * SMRT Link v13.0 release + + * 4.0.0 * Rename `isoseq3` to `isoseq` * Add new tool `isoseq cluster2` * Update `--max-5p-diff` default value for `isoseq collapse` diff --git a/docs/classification/pigeon-input.md b/docs/classification/pigeon-input.md index 134ac55..2635d32 100644 --- a/docs/classification/pigeon-input.md +++ b/docs/classification/pigeon-input.md @@ -8,6 +8,7 @@ nav_order: 4 ## Pigeon Tools Input **prepare** + | File | Description | | ----------- | ---- | | Transcript GFF | Collapsed transcript GFF from _isoseq collapse_ | @@ -16,6 +17,7 @@ nav_order: 4 | Intropolis data (Optional) | Intropolis data in custom format | **classify** + | File | Description | | ----------- | ---- | | Reference Genome | Reference genome in FASTA format | @@ -27,6 +29,7 @@ nav_order: 4 | FL Counts (Optional) | Bulk IsoSeq FL count info from _isoseq collapse_ (`*.flnc_counts.txt`) | **make-seurat** + | File | Description | | ----------- | ---- | | Molecules FASTA | Molecules FASTA file from _isoseq dedup_ | @@ -35,4 +38,4 @@ nav_order: 4 ### Examples Sorted and indexed references for human and mouse can be found [here](https://downloads.pacbcloud.com/public/dataset/MAS-Seq/REF-pigeon_ref_sets/). -Guidance about custom references can be found [here] (/classification/pigeon-annotation). +Guidance about custom references can be found [here](/classification/pigeon-annotation). diff --git a/docs/clustering/cli-workflow.md b/docs/clustering/cli-workflow.md index 9e15560..3cb463f 100644 --- a/docs/clustering/cli-workflow.md +++ b/docs/clustering/cli-workflow.md @@ -36,10 +36,12 @@ sequences in this [lima Iso-Seq FAQ](https://lima.how/faq/isoseq). $ lima movieX.hifi_reads.bam barcoded_primers.fasta movieX.fl.bam --isoseq --peek-guess If your sample is barcoded at the cDNA level, use `--overwrite-biosample-names` to update the sample names. + $ lima movieX.hifi_reads.bam barcoded_primers.fasta movieX.fl.bam --isoseq --peek-guess --overwrite-biosample-names **Example 1:** Following is the IsoSeq_v2_primers_12.fasta for the MAS-Seq bulk Iso-Seq primers that supports 12-plex barcoding at the cDNA level: + >IsoSeqX_bc01_5p CTACACGACGCTCTTCCGATCTACTACACGCAATGAAGTCGCAGGGTTGGG >IsoSeqX_bc02_5p