From 9458dac842f77252744c65f6e82deb81bf94203e Mon Sep 17 00:00:00 2001 From: Mahsa Date: Sun, 21 Jul 2024 12:35:30 +0330 Subject: [PATCH] README file --- README.html | 7 +++++++ README.md | 10 +++++++++- 2 files changed, 16 insertions(+), 1 deletion(-) diff --git a/README.html b/README.html index e351a9e..366bed6 100644 --- a/README.html +++ b/README.html @@ -465,6 +465,13 @@

DEG analysis

function in logarithm scale

{r} fit <- lmFit(Ex, design = design) contrast <- makeContrasts(non_V600E-v600e, levels = design) fit2 <- contrasts.fit(fit = fit, contrasts = contrast) fit2 <- eBayes(fit2) deg <- topTable(fit2, number = Inf)

+
                  logFC  AveExpr         t      P.Value    adj.P.Val        B 
+ENSG00000114302  2.059983 9.016187  9.425437 5.230565e-18 7.353333e-14 30.26257    
+ENSG00000135709 -1.866375 2.860313 -9.361123 8.118053e-18 7.353333e-14 29.83572   
+ENSG00000106733 -1.558516 2.832475 -9.152146 3.356666e-17 1.843978e-13 28.45755      
+ENSG00000157954 -2.057048 2.989810 -9.123564 4.071489e-17 1.843978e-13 28.27013      
+ENSG00000164944  1.905614 7.507759  9.077754 5.544899e-17 2.009028e-13 27.97029      
+ENSG00000187098 -1.353065 2.716279 -9.004784 9.056078e-17 2.533801e-13 27.49412
diff --git a/README.md b/README.md index 6471062..586a274 100644 --- a/README.md +++ b/README.md @@ -133,10 +133,18 @@ fit2 <- eBayes(fit2) deg <- topTable(fit2, number = Inf) ``` +``` + logFC AveExpr t P.Value adj.P.Val B +ENSG00000114302 2.059983 9.016187 9.425437 5.230565e-18 7.353333e-14 30.26257 +ENSG00000135709 -1.866375 2.860313 -9.361123 8.118053e-18 7.353333e-14 29.83572 +ENSG00000106733 -1.558516 2.832475 -9.152146 3.356666e-17 1.843978e-13 28.45755 +ENSG00000157954 -2.057048 2.989810 -9.123564 4.071489e-17 1.843978e-13 28.27013 +ENSG00000164944 1.905614 7.507759 9.077754 5.544899e-17 2.009028e-13 27.97029 +ENSG00000187098 -1.353065 2.716279 -9.004784 9.056078e-17 2.533801e-13 27.49412 +``` -![](https://github.com/Mahsa-Ehsanifard/GEO_microarray/blob/main/voom.png?raw=true)