diff --git a/README.md b/README.md
index f9dbf69..f38d8e0 100644
--- a/README.md
+++ b/README.md
@@ -215,7 +215,8 @@ The **MetatONTrack.sh** script reproduces what the EPI2ME 16S workflow does, bla
 
 Usage: MetatONTrack.sh \<fastq reads\> \<min num reads\>
 
-Note: set _BLASTN_, _SEQTK_ and _DB_ variables to blastn, seqtk executables and to an NCBI Blast-indexed database respectively inside the script; if using NCBI nt database, change _cut -d" " -f2,3_ (line 47) and _cut -d" " -f2_ (line 48) to _cut -d" " -f1,2_ and _cut -d" " -f1_ respectively
+Note: Activate the virtual environment with ```source activate ONTrack_env``` before running.
+Set _DB_ variable to an NCBI Blast-indexed database inside the script; if using NCBI nt database, change _cut -d" " -f2,3_ (line 47) and _cut -d" " -f2_ (line 48) to _cut -d" " -f1,2_ and _cut -d" " -f1_ respectively
 
 Inputs:
 * \<fastq reads\>:  MinION fastq reads from a meta-barcoding experiment