diff --git a/README.md b/README.md index 7ccdea1..0712c21 100644 --- a/README.md +++ b/README.md @@ -35,16 +35,19 @@ Then, after completing _ONTrack_ installation, set the _BASECALLER_DIR_ variable * NCBI nt database (optional, in case you want to perform a local Blast analysis of your consensus sequences). -For downloading the database (~65 GB): +For downloading the database (~210 GB): ``` mkdir NCBI_nt_db cd NCBI_nt_db echo `date +%Y-%m-%d` > download_date.txt wget ftp://ftp.ncbi.nlm.nih.gov/blast/db/nt* -targz_files=$(find . | grep \.tar\.gz$ | sed 's/\.\///g') -for f in $targz_files; do tar -xzvf $f; done -rm $targz_files +targz_files=$(find . | grep "\\.tar\\.gz$") +for f in $targz_files; do + tar -xzvf $f; + rm $f; + rm $f".md5"; +done ``` Then, after completing the _ONTrack_ installation, set the _NTDB_ variable in **config_MinION_mobile_lab.R** to the full path to NCBI_nt_db/nt