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Documentation for Sapkota et. al. 2019

Data

BLUEs_pheno_all.csv - Best linear unbiased estimate (BLUE) phenotype data for all accessions in the panel.

PI Subpopulation Cluster Race Origin DTA PH GN GW GY FLH PL BL
PI152651 Caudatum 4 0 NA 66 146.61 1286 27.32 43.95 97 14.33333333 57.95
PI17548 Kafir 2 0 NA 66 214.06 1167 15.62 26.66 156.8333333 22.83333333 83.41666667
PI24969 Durra 3 0 NA 80 182.06 1319 29.92 50.86 162 13.33333333 41.83333333
PI329435 Mixed 1 0 NA 80 95.5 1388 15.68 30.47 65.83333333 26 72.58333333

Qmatrix_admixture_k5.csv

Ancestry coefficients of five subpopulations calculated using admixture (Alexandre et. al. 2009).

SAP_geno-pheno.zip

A zip file containing both genotype matrix (-1,0,1 format) and phenotypic BLUEs.

Manuscript

Manuscript for the publication along with supplementary file. The figures and tables are embedded in the manuscript.

Scripts

Package versions used

lme4_1.1-21 MCMCglmm_2.29 ape_5.3 coda_0.19-2 Matrix_1.2-17 BGLR_1.0.8 rrBLUP_4.6

VCF_to_genotype_matrix.R

This script contains the functions my.read.vcf to read SNP files in vcf format, and the parse.vcf function to create a genotype matrix (in -1,0,1 format) that can be used in the rrBLUP package.

Stratified_Sampling_SAP_cluster.R

R script to create a cross-validation file with individuals proportionally divided from each cluster into five equal folds.

Cross-validation scripts

R scripts used to implement GBLUP and cross validations using kin.blup function in R package rrBLUP.

  • CV1_prediction.R # proportional sampling from races
  • CV2_AR_prediction.R # sampling from across race
  • CV2_WR_prediction.R # sampling from within race

MCMCglmm_CV1_vCov.R

Fits multi-response model in MCMCglmm to calculate variance-covariance components due to conditional expectations from race.

Heterozygosity_Pegas.R

R script to calculate heterozygosity per site using R package Pegas.

LD_Calculation_HillandWeir.R

Calculations for expected values of R^2 under drift equilibrium Hill and Weir (1988). As implemented in Remington et al. (2001).

GenomicHeritability.R

Calculates variance components and genomic heritability