diff --git a/ModernWpf.Controls/CommandBarFlyout/CommandBarFlyoutToolBar.cs b/ModernWpf.Controls/CommandBarFlyout/CommandBarFlyoutToolBar.cs
index 64456daa..e28cef9f 100644
--- a/ModernWpf.Controls/CommandBarFlyout/CommandBarFlyoutToolBar.cs
+++ b/ModernWpf.Controls/CommandBarFlyout/CommandBarFlyoutToolBar.cs
@@ -633,7 +633,15 @@ void EnsureAutomationSetCountAndPosition()
             {
                 if (command is UIElement commandAsUIElement)
                 {
-                    if (commandAsUIElement.Visibility == Visibility.Visible)
+                    // Don't count AppBarSeparator if IsTabStop is false
+                    if (commandAsUIElement is AppBarSeparator separator)
+                    {
+                        if (!separator.IsTabStop)
+                        {
+                            continue;
+                        }
+                    }
+                    else if (commandAsUIElement.Visibility == Visibility.Visible)
                     {
                         sizeOfSet++;
                     }
@@ -650,7 +658,15 @@ void EnsureAutomationSetCountAndPosition()
             {
                 if (command is UIElement commandAsUIElement)
                 {
-                    if (commandAsUIElement.Visibility == Visibility.Visible)
+                    // Don't count AppBarSeparator if IsTabStop is false
+                    if (commandAsUIElement is AppBarSeparator separator)
+                    {
+                        if (!separator.IsTabStop)
+                        {
+                            continue;
+                        }
+                    }
+                    else if (commandAsUIElement.Visibility == Visibility.Visible)
                     {
                         AutomationProperties.SetSizeOfSet(commandAsUIElement, sizeOfSet);
                     }
@@ -868,7 +884,7 @@ bool IsControlFocusable(
             return control != null &&
                 control.Visibility == Visibility.Visible &&
                 control.IsEnabled &&
-                (!checkTabStop || control.IsTabStop);
+                (control.IsTabStop || (!checkTabStop && !(control is AppBarSeparator))); // AppBarSeparator is not focusable if IsTabStop is false
         }
 
         Control GetFirstTabStopControl(
diff --git a/test/ModernWpfTestApp/CommandBarFlyoutPage.xaml b/test/ModernWpfTestApp/CommandBarFlyoutPage.xaml
index 5a4de40f..dd5e2999 100644
--- a/test/ModernWpfTestApp/CommandBarFlyoutPage.xaml
+++ b/test/ModernWpfTestApp/CommandBarFlyoutPage.xaml
@@ -23,6 +23,7 @@
                 Opened="OnFlyoutOpened"
                 Closed="OnFlyoutClosed">
                 <ui:AppBarButton x:Name="CutButton1" AutomationProperties.AutomationId="CutButton1" Label="Cut" Icon="Cut" Click="OnElementClicked" />
+                <AppBarSeparator />
                 <ui:AppBarButton x:Name="CopyButton1" AutomationProperties.AutomationId="CopyButton1" Label="Copy" Icon="Copy" Click="OnElementClicked" />
                 <ui:AppBarButton x:Name="PasteButton1" AutomationProperties.AutomationId="PasteButton1" Label="Paste" Icon="Paste" Click="OnElementClicked" />
                 <ui:AppBarButton x:Name="BoldButton1" AutomationProperties.AutomationId="BoldButton1" Label="Bold" Icon="Bold" Click="OnElementClicked" />
@@ -30,6 +31,7 @@
                 <ui:AppBarToggleButton x:Name="UnderlineButton1" AutomationProperties.AutomationId="UnderlineButton1" Label="Underline" Icon="Underline" Click="OnElementClicked" />
                 <muxc:CommandBarFlyout.SecondaryCommands>
                     <ui:AppBarButton x:Name="UndoButton1" AutomationProperties.AutomationId="UndoButton1" Label="Undo" Icon="Undo" Click="OnElementClicked" />
+                    <AppBarSeparator IsTabStop="False" />
                     <ui:AppBarButton x:Name="RedoButton1" AutomationProperties.AutomationId="RedoButton1" Label="Redo" Icon="Redo" Click="OnElementClicked" />
                     <ui:AppBarButton x:Name="SelectAllButton1" AutomationProperties.AutomationId="SelectAllButton1" Label="Select all" Click="OnElementClicked" />
                     <ui:AppBarToggleButton x:Name="FavoriteToggleButton1" AutomationProperties.AutomationId="FavoriteToggleButton1" Label="Favorite" Icon="Favorite" Checked="OnElementChecked" Unchecked="OnElementUnchecked" />