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Hello!@JianjunJin,
I try to use hifiasm result to assembling the chloroplast genome.Here is my codes
get_organelle_config.py -a embplant_pt,embplant_mt --use-local ./GetOrganelleDB/0.0.1 get_organelle_from_assembly.py -g ./lh_green.hic.p_utg.gfa -o plastome_output -F embplant_pt --max-depth 100
I get the error
2024-06-05 09:28:18,671 - INFO: Disentangling plastome_output/slimmed_assembly_graph.gfa as a circular genome ... 2024-06-05 09:28:18,692 - ERROR: Traceback (most recent call last): File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 815, in extract_organelle_genome disentangle_assembly(fastg_file=slim_out_fg, blast_db_base=blast_db, mode=organelle_type, File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 798, in disentangle_assembly disentangle_inside(fastg_f=fastg_file, tab_f=tab_file, o_p=output, w_f=weight_factor, log_in=log_dis, File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/pipe_control_func.py", line 139, in func_modified r = func(*args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 619, in disentangle_inside target_results = input_graph.find_target_graph(tab_f, ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 2478, in find_target_graph new_assembly.filter_by_coverage(database_n=database_name, File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 1749, in filter_by_coverage removing_low_cov = [candidate_v ^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 1751, in <listcomp> if abs(log(self.vertex_info[candidate_v].cov / max_tagged_cov)) > log_hard_cov_threshold] ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ ValueError: math domain error 2024-06-05 09:28:18,695 - ERROR: Traceback (most recent call last): File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 1043, in main ext_res = extract_organelle_genome(out_base=options.output_base, ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 845, in extract_organelle_genome raise e File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 815, in extract_organelle_genome disentangle_assembly(fastg_file=slim_out_fg, blast_db_base=blast_db, mode=organelle_type, File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 798, in disentangle_assembly disentangle_inside(fastg_f=fastg_file, tab_f=tab_file, o_p=output, w_f=weight_factor, log_in=log_dis, File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/pipe_control_func.py", line 139, in func_modified r = func(*args, **kwargs) ^^^^^^^^^^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/bin/get_organelle_from_assembly.py", line 619, in disentangle_inside target_results = input_graph.find_target_graph(tab_f, ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 2478, in find_target_graph new_assembly.filter_by_coverage(database_n=database_name, File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 1749, in filter_by_coverage removing_low_cov = [candidate_v ^^^^^^^^^^^^ File "/home/liuli/anaconda3/envs/py3.11/lib/python3.11/site-packages/GetOrganelleLib/assembly_parser.py", line 1751, in <listcomp> if abs(log(self.vertex_info[candidate_v].cov / max_tagged_cov)) > log_hard_cov_threshold] ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ ValueError: math domain error
Here is my log file get_org.log.txt
Any help would be appreciated! Best, Zhanwu
The text was updated successfully, but these errors were encountered:
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Hello!@JianjunJin,
I try to use hifiasm result to assembling the chloroplast genome.Here is my codes
I get the error
Here is my log file
get_org.log.txt
Any help would be appreciated!
Best,
Zhanwu
The text was updated successfully, but these errors were encountered: