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Asking for friend... |
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Replies: 1 comment
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Haha...aren't we all asking for a friend? Thanks so much for your question! eFluor 615 is great for multiplexed 2D imaging but the signal is still present after prolonged exposure (120+ minutes) of 1 mg/ml LiBH4. It behaves very similar to Alexa Fluor 594 and can be placed in the last cycle of an IBEX experiment or used as a fiducial. To find the inactivation conditions of a particular fluorophore/dye, please go to the Reagent Resources table as shown in the attached picture or go to this link that displays all the fluorescent probes tested by the IBEX Imaging Community.
Best of luck! |
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Haha...aren't we all asking for a friend?
Thanks so much for your question! eFluor 615 is great for multiplexed 2D imaging but the signal is still present after prolonged exposure (120+ minutes) of 1 mg/ml LiBH4. It behaves very similar to Alexa Fluor 594 and can be placed in the last cycle of an IBEX experiment or used as a fiducial.
To find the inactivation conditions of a particular fluorophore/dye, please go to the Reagent Resources table as shown in the attached picture or go to this link that displays all the fluorescent probes tested by the IBEX Imaging Community.
Best of luck!
A