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ERC000027

ERC000027: ENA Micro B3

Minimum information about a Micro B3 sample. A checklist for reporting metadata of marine microbial samples associated with genomics data. NOTE: Non-genomics data, i.e. oceanographic environmental data and morphology-based biodiversity data, should be submitted to the appropriate National Oceanographic Data Centre according to established reporting practices maintained by oceanographic community experts. Major National Oceanographic Data Centres from countries bordering the North-East Atlantic, and its adjacent seas: the Mediterranean, the Black Sea, the Baltic, the North Sea and the Arctic are listed at http://www.seadatanet.org/Overview/Partners. For the Ocean Sampling Day campaign, non-genomics data shall be reported to the PANGAEA (http://www.pangaea.de/submit/).

Study

A Study is a container for a sequencing investigation that may comprise multiple experiments. The Study has an overall goal, but is otherwise minimally defined in the SRA. A Study is composed of a descriptor, zero or more experiments, and zero or more analyses. The submitter may decorate the Study with web links and properties.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for a study. this is used to link experiments to the study.
title mandatory Title of the study as would be used in a publication.
study_type mandatory The study_type presents a controlled vocabulary for expressing the overall purpose of the study. Whole Genome Sequencing, Metagenomics, Transcriptome Analysis, Resequencing, Epigenetics, Synthetic Genomics, Forensic or Paleo-genomics, Gene Regulation Study, Cancer Genomics, Population Genomics, RNASeq, Exome Sequencing, Pooled Clone Sequencing, Transcriptome Sequencing, Other
new_study_type optional Optional if 'study_type' is not 'other'. to propose a new term, select other and enter a new study type.
study_abstract optional Briefly describes the goals, purpose, and scope of the study. this need not be listed if it can be inherited from a referenced publication.

Experiment

An experiment object serves as a metadata record encapsulating essential details about a sequencing experiment, including the experimental design, sequencing type, and relevant parameters. This information enhances the interpretation and contextual understanding of nucleotide sequences submitted to the archive.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each experiment. this is used to link runs to experiments.
title mandatory Short text that can be used to call out experiment records in searches or in displays. this element is technically optional but should be used for all new records.
study_alias mandatory Identifies the parent study. (from study metadata)
sample_alias mandatory (from sample metadata)
design_description mandatory Goal and setup of the individual library including library was constructed.
library_name optional The submitter's name for this library.
library_strategy mandatory Sequencing technique intended for this library. WGS, WGA, WXS, RNA-Seq, ssRNA-seq, snRNA-seq, miRNA-Seq, ncRNA-Seq, FL-cDNA, EST, Hi-C, ATAC-seq, WCS, RAD-Seq, CLONE, POOLCLONE, AMPLICON, CLONEEND, FINISHING, ChIP-Seq, MNase-Seq, DNase-Hypersensitivity, Bisulfite-Seq, CTS, MRE-Seq, MeDIP-Seq, MBD-Seq, Tn-Seq, VALIDATION, FAIRE-seq, SELEX, RIP-Seq, ChIA-PET, Synthetic-Long-Read, Targeted-Capture, Tethered Chromatin Conformation Capture, NOMe-Seq, ChM-Seq, GBS, Ribo-Seq, OTHER
library_source mandatory The library_source specifies the type of source material that is being sequenced. GENOMIC, GENOMIC SINGLE CELL, TRANSCRIPTOMIC, TRANSCRIPTOMIC SINGLE CELL, METAGENOMIC, METATRANSCRIPTOMIC, SYNTHETIC, VIRAL RNA, OTHER
library_selection mandatory Method used to enrich the target in the sequence library preparation RANDOM, PCR, RANDOM PCR, RT-PCR, HMPR, MF, repeat fractionation, size fractionation, MSLL, cDNA, cDNA_randomPriming, cDNA_oligo_dT, PolyA, Oligo-dT, Inverse rRNA, Inverse rRNA selection, ChIP, ChIP-Seq, MNase, DNase, Hybrid Selection, Reduced Representation, Restriction Digest, 5-methylcytidine antibody, MBD2 protein methyl-CpG binding domain, CAGE, RACE, MDA, padlock probes capture method, other, unspecified
library_layout mandatory Library_layout specifies whether to expect single, paired, or other configuration of reads. in the case of paired reads, information about the relative distance and orientation is specified.
insert_size optional Insert size for paired reads
library_construction_protocol optional Free form text describing the protocol by which the sequencing library was constructed.
platform mandatory The platform record selects which sequencing platform and platform-specific runtime parameters. this will be determined by the center. optional if 'instrument_model' is provided. LS454, ILLUMINA, HELICOS, ABI_SOLID, COMPLETE_GENOMICS, BGISEQ, OXFORD_NANOPORE, PACBIO_SMRT, ION_TORRENT, CAPILLARY, DNBSEQ, ELEMENT, ULTIMA, VELA_DIAGNOSTICS, GENAPSYS, GENEMIND, TAPESTRI
instrument_model mandatory Model of the sequencing instrument. 454 GS, 454 GS 20, 454 GS FLX, 454 GS FLX Titanium, 454 GS FLX+, 454 GS Junior, AB 310 Genetic Analyzer, AB 3130 Genetic Analyzer, AB 3130xL Genetic Analyzer, AB 3500 Genetic Analyzer, AB 3500xL Genetic Analyzer, AB 3730 Genetic Analyzer, AB 3730xL Genetic Analyzer, AB 5500 Genetic Analyzer, AB 5500xl Genetic Analyzer, AB 5500xl-W Genetic Analysis System, AB SOLiD 3 Plus System, AB SOLiD 4 System, AB SOLiD 4hq System, AB SOLiD PI System, AB SOLiD System, AB SOLiD System 2.0, AB SOLiD System 3.0, BGISEQ-50, BGISEQ-500, Complete Genomics, DNBSEQ-G400, DNBSEQ-G400 FAST, DNBSEQ-G50, DNBSEQ-T7, Element AVITI, FASTASeq 300, GENIUS, GS111, Genapsys Sequencer, GenoCare 1600, GenoLab M, GridION, Helicos HeliScope, HiSeq X Five, HiSeq X Ten, Illumina Genome Analyzer, Illumina Genome Analyzer II, Illumina Genome Analyzer IIx, Illumina HiScanSQ, Illumina HiSeq 1000, Illumina HiSeq 1500, Illumina HiSeq 2000, Illumina HiSeq 2500, Illumina HiSeq 3000, Illumina HiSeq 4000, Illumina HiSeq X, Illumina MiSeq, Illumina MiniSeq, Illumina NovaSeq 6000, Illumina NovaSeq X, Illumina iSeq 100, Ion GeneStudio S5, Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, Ion Torrent Genexus, Ion Torrent PGM, Ion Torrent Proton, Ion Torrent S5, Ion Torrent S5 XL, MGISEQ-2000RS, MinION, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, Onso, PacBio RS, PacBio RS II, PromethION, Revio, Sentosa SQ301, Sequel, Sequel II, Sequel IIe, Tapestri, UG 100, unspecified

Run

A run contains a group of reads generated for a particular experiment.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each run.
experiment_alias mandatory From_experiment_metadata
file_name mandatory The name or relative pathname of a run data file.
file_format mandatory The run data file model. sra, srf, sff, fastq, fasta, tab, 454_native, 454_native_seq, 454_native_qual, Helicos_native, Illumina_native, Illumina_native_seq, Illumina_native_prb, Illumina_native_int, Illumina_native_qseq, Illumina_native_scarf, SOLiD_native, SOLiD_native_csfasta, SOLiD_native_qual, PacBio_HDF5, bam, cram, CompleteGenomics_native, OxfordNanopore_native

Sample

A Sample defines an isolate of sequenceable material upon which sequencing experiments can be based. The Sample object may be a surrogate for taxonomy accession or an anonymized individual identifier. Or, it may fully specify provenance and isolation method of the starting material.

Field name Cardinality Description Controlled vocabulary
alias mandatory Unique identificator for each sample.
title mandatory Short text that can be used to call out sample records in search results or in displays.
taxon_id mandatory Ncbi taxonomy identifier. this is appropriate for individual organisms and some environmental samples.
sample_description optional Free-form text describing the sample, its origin, and its method of isolation.
Latitude Start mandatory Latitude of the location where the sampling event started, e.g. each ctd cast, net tow, or bucket collection is a distinct event. format: ##.####, decimal degrees; north= +, south= -; use wgs 84 for gps data. example: -24.6666. (Units: DD)
Longitude Start mandatory Longitude of the location where the sampling event started, e.g. each ctd cast, net tow, or bucket collection is a distinct event. format: ###.####, decimal degrees; east= +, west= -; use wgs 84 for gps data. example: -096.1012. (Units: DD)
Latitude End recommended Latitude of the location where the sampling event ended, e.g. each ctd cast, net tow, or bucket collection is a distinct event. format: ##.####, decimal degrees; north= +, south= -; use wgs 84 for gps data. example: -24.6643. (Units: DD)
Longitude End recommended Longitude of the location where the sampling event ended, e.g. each ctd cast, net tow, or bucket collection is a distinct event. format: ###.####, decimal degrees; east= +, west= -; use wgs 84 for gps data. example: -096.1171. (Units: DD)
Protocol Label mandatory Identifies the protocol used to produce the sample, e.g. filtration and preservation. example: bact_nuc_w0.22-1.6.
trophic level optional Trophic levels are the feeding position in a food chain. microbes can be a range of producers (e.g. chemolithotroph) autotroph, carboxydotroph, chemoautotroph, chemoheterotroph, chemolithoautotroph, chemolithotroph, chemoorganoheterotroph, chemoorganotroph, chemosynthetic, chemotroph, copiotroph, diazotroph, facultative autotroph, heterotroph, lithoautotroph, lithoheterotroph, lithotroph, methanotroph, methylotroph, mixotroph, obligate chemoautolithotroph, oligotroph, organoheterotroph, organotroph, photoautotroph, photoheterotroph, photolithoautotroph, photolithotroph, photosynthetic, phototroph
observed biotic relationship optional Description of relationship(s) between the subject organism and other organism(s) it is associated with. e.g., parasite on species x; mutualist with species y. the target organism is the subject of the relationship, and the other organism(s) is the object. commensal, free living, mutualism, parasite, symbiont
known pathogenicity optional To what is the entity pathogenic, for instance plant, fungi, bacteria
relationship to oxygen optional Is this organism an aerobe, anaerobe? please note that aerobic and anaerobic are valid descriptors for microbial environments aerobe, anaerobe, facultative, microaerophilic, microanaerobe, obligate aerobe, obligate anaerobe
propagation optional The type of reproduction from the parent stock. values for this field is specific to different taxa. for phage or virus: lytic/lysogenic/temperate/obligately lytic. for plasmids: incompatibility group. for eukaryotes: sexual/asexual. mandatory for migs of eukayotes, plasmids and viruses.
sample collection method optional The method employed for collecting the sample. can be provided in the form of a pmid, doi, url or text.
sample storage temperature optional Temperature at which sample was stored, e.g. -80 (Units: °C)
sample storage location optional Location at which sample was stored, usually name of a specific freezer/room. indicate the location name.
oxygenation status of sample optional Oxygenation status of sample aerobic, anaerobic
density optional Density of sample (Units: g/m3)
project name mandatory Name of the project within which the sequencing was organized
finishing strategy optional Was the genome project intended to produce a complete or draft genome, coverage, the fold coverage of the sequencing expressed as 2x, 3x, 18x etc, and how many contigs were produced for the genome. mandatory for migs of eukaryote, bacteria and archaea.
ploidy optional The ploidy level of the genome (e.g. allopolyploid, haploid, diploid, triploid, tetraploid). it has implications for the downstream study of duplicated gene and regions of the genomes (and perhaps for difficulties in assembly). for terms, please select terms listed under class ploidy (pato:001374) of phenotypic quality ontology (pato), and for a browser of pato (v 2018-03-27) please refer to http://purl.bioontology.org/ontology/pato
number of replicons optional Reports the number of replicons in a nuclear genome of eukaryotes, in the genome of a bacterium or archaea or the number of segments in a segmented virus. always applied to the haploid chromosome count of a eukaryote. mandatory for migs of eukaryotes, bacteria, archaea and segmented virus.
extrachromosomal elements optional Do plasmids exist of significant phenotypic consequence (e.g. ones that determine virulence or antibiotic resistance). megaplasmids? other plasmids (borrelia has 15+ plasmids).
estimated size optional The estimated size of the genome (in bp) prior to sequencing. of particular importance in the sequencing of (eukaryotic) genome which could remain in draft form for a long or unspecified period. mandatory for migs of eukaryotes.
target gene optional Targeted gene or locus name for marker gene studies
target subfragment optional Name of subfragment of a gene or locus. important to e.g. identify special regions on marker genes like v6 on 16s rrna
multiplex identifiers optional Molecular barcodes, called multiplex identifiers (mids), that are used to specifically tag unique samples in a sequencing run. sequence should be reported in uppercase letters
sequence quality check optional Indicate if the sequence has been called by automatic systems (none) or undergone a manual editing procedure (e.g. by inspecting the raw data or chromatograms). applied only for sequences that are not submitted to sra or dra manual, none, software
chimera check software optional Tool(s) used for chimera checking, including version number and parameters, to discover and remove chimeric sequences. a chimeric sequence is comprised of two or more phylogenetically distinct parent sequences.
relevant electronic resources optional A related resource that is referenced, cited, or otherwise associated to the sequence in the format of a pmid, doi or url
relevant standard operating procedures optional Standard operating procedures used in assembly and/or annotation of genomes, metagenomes or environmental sequences in the format of a pmid, doi or url
environmental package mandatory Migs/mims/mimarks extension for reporting of measurements and observations obtained from one or more of the environments where the sample was obtained. all environmental packages listed here are further defined in separate subtables. by giving the name of the environmental package, a selection of fields can be made from the subtables and can be reported water
collection date mandatory The date the sample was collected with the intention of sequencing, either as an instance (single point in time) or interval. in case no exact time is available, the date/time can be right truncated i.e. all of these are valid iso8601 compliant times: 2008-01-23t19:23:10+00:00; 2008-01-23t19:23:10; 2008-01-23; 2008-01; 2008.
geographic location (country and/or sea) mandatory The geographical origin of where the sample was collected from, with the intention of sequencing, as defined by the country or sea name. country or sea names should be chosen from the insdc country list (http://insdc.org/country.html). Afghanistan, Albania, Algeria, American Samoa, Andorra, Angola, Anguilla, Antarctica, Antigua and Barbuda, Arctic Ocean, Argentina, Armenia, Aruba, Ashmore and Cartier Islands, Atlantic Ocean, Australia, Austria, Azerbaijan, Bahamas, Bahrain, Baker Island, Baltic Sea, Bangladesh, Barbados, Bassas da India, Belarus, Belgium, Belize, Benin, Bermuda, Bhutan, Bolivia, Borneo, Bosnia and Herzegovina, Botswana, Bouvet Island, Brazil, British Virgin Islands, Brunei, Bulgaria, Burkina Faso, Burundi, Cambodia, Cameroon, Canada, Cape Verde, Cayman Islands, Central African Republic, Chad, Chile, China, Christmas Island, Clipperton Island, Cocos Islands, Colombia, Comoros, Cook Islands, Coral Sea Islands, Costa Rica, Cote d'Ivoire, Croatia, Cuba, Curacao, Cyprus, Czechia, Czech Republic, Democratic Republic of the Congo, Denmark, Djibouti, Dominica, Dominican Republic, East Timor, Ecuador, Egypt, El Salvador, Equatorial Guinea, Eritrea, Estonia, Ethiopia, Europa Island, Falkland Islands (Islas Malvinas), Faroe Islands, Fiji, Finland, France, French Guiana, French Polynesia, French Southern and Antarctic Lands, Gabon, Gambia, Gaza Strip, Georgia, Germany, Ghana, Gibraltar, Glorioso Islands, Greece, Greenland, Grenada, Guadeloupe, Guam, Guatemala, Guernsey, Guinea, Guinea-Bissau, Guyana, Haiti, Heard Island and McDonald Islands, Honduras, Hong Kong, Howland Island, Hungary, Iceland, India, Indian Ocean, Indonesia, Iran, Iraq, Ireland, Isle of Man, Israel, Italy, Jamaica, Jan Mayen, Japan, Jarvis Island, Jersey, Johnston Atoll, Jordan, Juan de Nova Island, Kazakhstan, Kenya, Kerguelen Archipelago, Kingman Reef, Kiribati, Kosovo, Kuwait, Kyrgyzstan, Laos, Latvia, Lebanon, Lesotho, Liberia, Libya, Liechtenstein, Lithuania, Luxembourg, Macau, Macedonia, Madagascar, Malawi, Malaysia, Maldives, Mali, Malta, Marshall Islands, Martinique, Mauritania, Mauritius, Mayotte, Mediterranean Sea, Mexico, Micronesia, Midway Islands, Moldova, Monaco, Mongolia, Montenegro, Montserrat, Morocco, Mozambique, Myanmar, Namibia, Nauru, Navassa Island, Nepal, Netherlands, New Caledonia, New Zealand, Nicaragua, Niger, Nigeria, Niue, Norfolk Island, North Korea, North Sea, Northern Mariana Islands, Norway, Oman, Pacific Ocean, Pakistan, Palau, Palmyra Atoll, Panama, Papua New Guinea, Paracel Islands, Paraguay, Peru, Philippines, Pitcairn Islands, Poland, Portugal, Puerto Rico, Qatar, Republic of the Congo, Reunion, Romania, Ross Sea, Russia, Rwanda, Saint Helena, Saint Kitts and Nevis, Saint Lucia, Saint Pierre and Miquelon, Saint Vincent and the Grenadines, Samoa, San Marino, Sao Tome and Principe, Saudi Arabia, Senegal, Serbia, Seychelles, Sierra Leone, Singapore, Sint Maarten, Slovakia, Slovenia, Solomon Islands, Somalia, South Africa, South Georgia and the South Sandwich Islands, South Korea, Southern Ocean, Spain, Spratly Islands, Sri Lanka, Sudan, Suriname, Svalbard, Swaziland, Sweden, Switzerland, Syria, Taiwan, Tajikistan, Tanzania, Tasman Sea, Thailand, Togo, Tokelau, Tonga, Trinidad and Tobago, Tromelin Island, Tunisia, Turkey, Turkmenistan, Turks and Caicos Islands, Tuvalu, USA, Uganda, Ukraine, United Arab Emirates, United Kingdom, Uruguay, Uzbekistan, Vanuatu, Venezuela, Viet Nam, Virgin Islands, Wake Island, Wallis and Futuna, West Bank, Western Sahara, Yemen, Zambia, Zimbabwe, missing: control sample, missing: data agreement established pre-2023, missing: endangered species, missing: human-identifiable, missing: lab stock, missing: sample group, missing: synthetic construct, missing: third party data, not applicable, not collected, not provided, restricted access
broad-scale environmental context mandatory Report the major environmental system the sample or specimen came from. the system(s) identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done (e.g. in the desert or a rainforest). we recommend using subclasses of envo’s biome class: http://purl.obolibrary.org/obo/envo_00000428. envo documentation about how to use the field: https://github.com/environmentontology/envo/wiki/using-envo-with-mixs.
local environmental context mandatory Report the entity or entities which are in the sample or specimen’s local vicinity and which you believe have significant causal influences on your sample or specimen. we recommend using envo terms which are of smaller spatial grain than your entry for "broad-scale environmental context". terms, such as anatomical sites, from other obo library ontologies which interoperate with envo (e.g. uberon) are accepted in this field. envo documentation about how to use the field: https://github.com/environmentontology/envo/wiki/using-envo-with-mixs.
environmental medium mandatory Report the environmental material(s) immediately surrounding the sample or specimen at the time of sampling. we recommend using subclasses of 'environmental material' (http://purl.obolibrary.org/obo/envo_00010483). envo documentation about how to use the field: https://github.com/environmentontology/envo/wiki/using-envo-with-mixs . terms from other obo ontologies are permissible as long as they reference mass/volume nouns (e.g. air, water, blood) and not discrete, countable entities (e.g. a tree, a leaf, a table top).
Sampling Campaign mandatory Refers to a finite or indefinite activity aiming at collecting data/samples, e.g. a cruise, a time series, a mesocosm experiment. example: tara_20110401z.
Sampling Site mandatory Refers to the site/station where data/sample collection is performed. term list: osd site registry or sdn:c17, http://tinyurl.com/oxux985 example: poseidon-e1-m3a time series station.
Sampling Platform mandatory Refers to the unique stage from which the sampling device has been deployed. includes platform category from sdn:l06, http://seadatanet.maris2.nl/v_bodc_vocab_v2/search.asp?lib=l06, and platform name. example: research vessel tara.
amount or size of sample collected optional The total amount or size (volume (ml), mass (g) or area (m2) ) of sample collected. (Units: m3)
biomass optional Amount of biomass; should include the name for the part of biomass measured, e.g. microbial, total. can include multiple measurements (Units: t)
organism count optional Total cell count of any organism (or group of organisms) per gram, volume or area of sample, should include name of organism followed by count. the method that was used for the enumeration (e.g. qpcr, atp, mpn, etc.) should also be provided. (example: total prokaryotes; 3.5e7 cells per ml; qpcr)
Marine Region mandatory The geographical origin of the sample as defined by the marine region name chosen from the marine regions vocabulary at http://www.marineregions.org/. example: aegean sea.
water temperature mandatory Temperature of water at the time of taking the sample. format: ##.####, sdn:p02:75:temp, sdn:p06:46:upaa for °c. example: 17.7122. (Units: ºC)
sample storage duration optional Duration for which the sample was stored. indicate the duration for which the sample was stored written in iso 8601 format.
specific host optional If there is a host involved, please provide its taxid (or environmental if not actually isolated from the dead or alive host - i.e. pathogen could be isolated from a swipe of a bench etc) and report whether it is a laboratory or natural host). from this we can calculate any number of groupings of hosts (e.g. animal vs plant, all fish hosts, etc)
health or disease status of specific host optional Health or disease status of specific host at time of collection. this field accepts pato (v 2013-10-28) terms, for a browser please see http://purl.bioontology.org/ontology/pato
atmospheric data optional Measurement of atmospheric data; can include multiple data
conductivity optional Electrical conductivity of water (Units: mS/cm)
water current optional Measurement of magnitude and direction of flow within a fluid (Units: m3/s)
fluorescence optional Raw (volts) or converted (mg chla/m^3) fluorescence of the water (Units: mg Chla/m3)
light intensity optional Measurement of light intensity (Units: lux)
mean friction velocity optional Measurement of mean friction velocity (Units: m/s)
mean peak friction velocity optional Measurement of mean peak friction velocity (Units: m/s)
downward PAR optional Visible waveband radiance and irradiance measurements in the water column (Units: µE/m2/s)
photon flux optional Measurement of photon flux (Units: µmol/m2/s)
pressure optional Pressure to which the sample is subject, in atmospheres (Units: bar)
tidal stage optional Stage of tide high, low
turbidity optional Turbidity measurement (Units: NTU)
pH optional Ph measurement
total depth of water column optional Measurement of total depth of water column (Units: m)
alkyl diethers optional Concentration of alkyl diethers (Units: µg/L)
aminopeptidase activity optional Measurement of aminopeptidase activity (Units: mol/L/h)
ammonium optional Concentration of ammonium (Units: µmol/L)
bacterial carbon production optional Measurement of bacterial carbon production (Units: ng/h)
bacterial production optional Bacterial production in the water column measured by isotope uptake (Units: mg/m3/d)
bacterial respiration optional Measurement of bacterial respiration in the water column (Units: mg/m3/d)
bishomohopanol optional Concentration of bishomohopanol (Units: µg/g)
bromide optional Concentration of bromide (Units: µmol/L)
calcium optional Concentration of calcium (Units: µmol/L)
carbon/nitrogen ratio optional Ratio of amount or concentrations of carbon to nitrogen
chloride optional Concentration of chloride (Units: mg/L)
chlorophyll optional Concentration of chlorophyll (Units: µg/L)
diether lipids optional Concentration of diether lipids; can include multiple types of diether lipids (Units: ng/L)
dissolved carbon dioxide optional Concentration of dissolved carbon dioxide (Units: µmol/L)
dissolved hydrogen optional Concentration of dissolved hydrogen (Units: µmol/L)
dissolved inorganic carbon optional Dissolved inorganic carbon concentration (Units: µg/L)
dissolved inorganic nitrogen optional Concentration of dissolved inorganic nitrogen (Units: µg/L)
dissolved inorganic phosphorus optional Concentration of dissolved inorganic phosphorus (Units: µg/L)
dissolved organic carbon optional Concentration of dissolved organic carbon (Units: µmol/L)
dissolved organic nitrogen optional Dissolved organic nitrogen concentration measured as; total dissolved nitrogen - nh4 - no3 - no2 (Units: µg/L)
dissolved oxygen optional Concentration of dissolved oxygen (Units: µmol/kg)
glucosidase activity optional Measurement of glucosidase activity (Units: mol/L/h)
magnesium optional Concentration of magnesium (Units: parts/million)
n-alkanes optional Concentration of n-alkanes; can include multiple n-alkanes (Units: µmol/L)
nitrate optional Concentration of nitrate (Units: µmol/L)
nitrite optional Concentration of nitrite (Units: µmol/L)
total nitrogen concentration optional Concentration of nitrogen (total). total nitrogen concentration of water samples, calculated by: total nitrogen = total dissolved nitrogen + particulate nitrogen. can also be measured without filtering, reported as nitrogen (Units: µmol/L)
organic carbon optional Concentration of organic carbon (Units: µmol/L)
organic matter optional Concentration of organic matter (Units: µg/L)
organic nitrogen optional Concentration of organic nitrogen (Units: µg/L)
particulate organic carbon optional Concentration of particulate organic carbon (Units: µg/L)
particulate organic nitrogen optional Concentration of particulate organic nitrogen (Units: µg/L)
petroleum hydrocarbon optional Concentration of petroleum hydrocarbon (Units: µmol/L)
phaeopigments optional Concentration of phaeopigments; can include multiple phaeopigments (Units: µg/L)
phosphate optional Concentration of phosphate (Units: µmol/L)
phospholipid fatty acid optional Concentration of phospholipid fatty acids; can include multiple values (Units: mol/g)
potassium optional Concentration of potassium (Units: µmol/L)
primary production optional Measurement of primary production (Units: mg/m3/day)
redox potential optional Redox potential, measured relative to a hydrogen cell, indicating oxidation or reduction potential (Units: mV)