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I tried to use the external mask generated by ImageJ in the Segmentation step by choosing the External Segmentation option. Every thing before Step 4 runs normally. However, in Background Mask step it gives me error (shown at the end of this communication). The strange thing was that the program let me pass the step 4 once, for completely unknown reasons. Other times I just keep getting the same failures. I attach a test sample here: TestSample.zip
MATLAB Version 9.14.0.2286388 (R2023a) Update 3
MATLAB License Number: 334062
Operating System: Microsoft Windows 10 Enterprise Version 10.0 (Build 19044)
Java VM Version: Java 1.8.0_202-b08 with Oracle Corporation Java HotSpot(TM) 64-Bit Server VM mixed mode
Movie 1 - E:\Pengning\20241115 ChemoG Talin test\test\Results\movieData.mat:
-- Step 5: Background Mask
Intermediate brace '{}' indexing produced a comma-separated list with 0 values, but it must produce a single value when followed by subsequent indexing
operations.
Error in createMovieBackgroundMasks (line 191)
movieData.processes_{iP}.outFilePaths_(p.ChannelIndex(j)));
Error in Process/run (line 255)
obj.funName_(obj.getOwner(), varargin{:});
Error in packageGUI_RunFcn>userfcn_runProc_dfs (line 635)
userData.crtPackage.processes_{procID}.run(); % throws exception
Error in packageGUI_RunFcn>start_processing_movies_in_series (line 221)
userfcn_runProc_dfs(procID, procRun{iMovie}, handles); % user data is retrieved, updated and submitted
Error in packageGUI_RunFcn (line 166)
movieException = start_processing_movies_in_series(movieRun,handles,movieException,procRun);
Error in packageGUI_OpeningFcn>@(hObject,eventdata)packageGUI_RunFcn(hObject,eventdata,guidata(hObject)) (line 432)
set(handles.pushbutton_run, 'Callback', @(hObject,eventdata)packageGUI_RunFcn(hObject,eventdata,guidata(hObject)));
Please verify your settings are correct. Feel free to contact us if you have question regarding this error.
Please help us improve the software by clearly reporting the scenario when this error occurs, and the above error information to us (error information is also displayed in Matlab command line).
I also receive a warning in the end in the command window as well. I paste all the displayed messages below:
Saving results...
Finished Correcting!
Using previously specified correction image directories...
The folder E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\shade_corrected_images\shade_corrected_images_for_channel_1 already existed. Cleaning the folder ...
The folder E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\shade_corrected_images\shade_corrected_images_for_channel_2 already existed. Cleaning the folder ...
Starting shade correction...
Loading and processing correction image(s)...
Applying shade correction to images...
Shade correcting channel 1...
Correcting images from "E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\dark_current_corrected_images\dark_current_corrected_images_for_channel_1", resulting images will be stored in "E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\shade_corrected_images\shade_corrected_images_for_channel_1"
Using correction images from E:\Pengning\20241115 ChemoG Talin test\FakeShades
Shade correcting channel 2...
Correcting images from "E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\dark_current_corrected_images\dark_current_corrected_images_for_channel_2", resulting images will be stored in "E:\Pengning\20241115 ChemoG Talin test\test\Results\BiosensorsPackage\shade_corrected_images\shade_corrected_images_for_channel_2"
Using correction images from E:\Pengning\20241115 ChemoG Talin test\FakeShades
Saving results...
Finished Correcting!
Finished importing external data!
Warning: Cannot create background masks because some channels do not have foreground masks! Please segment these channels before creating background masks!
I tried to use the external mask generated by ImageJ in the Segmentation step by choosing the External Segmentation option. Every thing before Step 4 runs normally. However, in Background Mask step it gives me error (shown at the end of this communication). The strange thing was that the program let me pass the step 4 once, for completely unknown reasons. Other times I just keep getting the same failures. I attach a test sample here: TestSample.zip
MATLAB Version 9.14.0.2286388 (R2023a) Update 3
MATLAB License Number: 334062
Operating System: Microsoft Windows 10 Enterprise Version 10.0 (Build 19044)
Java VM Version: Java 1.8.0_202-b08 with Oracle Corporation Java HotSpot(TM) 64-Bit Server VM mixed mode
Movie 1 - E:\Pengning\20241115 ChemoG Talin test\test\Results\movieData.mat:
-- Step 5: Background Mask
Intermediate brace '{}' indexing produced a comma-separated list with 0 values, but it must produce a single value when followed by subsequent indexing
operations.
Error in createMovieBackgroundMasks (line 191)
movieData.processes_{iP}.outFilePaths_(p.ChannelIndex(j)));
Error in Process/run (line 255)
obj.funName_(obj.getOwner(), varargin{:});
Error in packageGUI_RunFcn>userfcn_runProc_dfs (line 635)
userData.crtPackage.processes_{procID}.run(); % throws exception
Error in packageGUI_RunFcn>start_processing_movies_in_series (line 221)
userfcn_runProc_dfs(procID, procRun{iMovie}, handles); % user data is retrieved, updated and submitted
Error in packageGUI_RunFcn (line 166)
movieException = start_processing_movies_in_series(movieRun,handles,movieException,procRun);
Error in packageGUI_OpeningFcn>@(hObject,eventdata)packageGUI_RunFcn(hObject,eventdata,guidata(hObject)) (line 432)
set(handles.pushbutton_run, 'Callback', @(hObject,eventdata)packageGUI_RunFcn(hObject,eventdata,guidata(hObject)));
Please verify your settings are correct. Feel free to contact us if you have question regarding this error.
Please help us improve the software by clearly reporting the scenario when this error occurs, and the above error information to us (error information is also displayed in Matlab command line).
For contact information please refer to the following URL:
http://www.utsouthwestern.edu/labs/danuser/software/
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