From fc8c81a14e77c86c854af4af04316ebf9bce172e Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 15:53:47 -0400 Subject: [PATCH 1/7] fix: update groovy code for running spooker from champagne --- lib/Utils.groovy | 31 ++++++++++++++++++++++++++----- 1 file changed, 26 insertions(+), 5 deletions(-) diff --git a/lib/Utils.groovy b/lib/Utils.groovy index db78a83..3fe6464 100644 --- a/lib/Utils.groovy +++ b/lib/Utils.groovy @@ -5,6 +5,30 @@ class Utils { def command_string = "spooker ${workflow.launchDir} ${pipeline_name}" def out = new StringBuilder() def err = new StringBuilder() + def spooker_in_path = check_command_in_path("spooker") + if (spooker_in_path) { + try { + println "Running spooker" + def command = command_string.execute() + command.consumeProcessOutput(out, err) + command.waitFor() + } catch(IOException e) { + err = e + } + new FileWriter("${workflow.launchDir}/log/spooker.log").with { + write("${out}\n${err}") + flush() + } + } else { + err = "spooker not found, skipping" + } + return err + } + // check whether a command is in the path + public static Boolean check_command_in_path(cmd) { + def command_string = "command -V ${cmd}" + def out = new StringBuilder() + def err = new StringBuilder() try { def command = command_string.execute() command.consumeProcessOutput(out, err) @@ -12,10 +36,7 @@ class Utils { } catch(IOException e) { err = e } - new FileWriter("${workflow.launchDir}/log/spooker.log").with { - write("${out}\n${err}") - flush() - } - return err + return err.length()==0 + } } From 85701a71994c94a46b07cce73e4f2369bcbceb87 Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 15:59:17 -0400 Subject: [PATCH 2/7] fix: biowulf has depreceated SLURM_JOBID in favor of SLURM_JOB_ID --- conf/biowulf.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/biowulf.config b/conf/biowulf.config index 2f9fb5a..79577e8 100644 --- a/conf/biowulf.config +++ b/conf/biowulf.config @@ -28,7 +28,7 @@ env.SINGULARITY_CACHEDIR = "/data/CCBR_Pipeliner/SIFS" process.clusterOptions = ' --gres=lscratch:200 ' -process.scratch = '/lscratch/$SLURM_JOBID' +process.scratch = '/lscratch/$SLURM_JOB_ID' process.stageInMode = 'symlink' process.stageOutMode = 'rsync' // for running pipeline on group sharing data directory, this can avoid inconsistent files timestamps From 9ee42ba500cfd45632df637d292ff90348fd9434 Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 16:00:56 -0400 Subject: [PATCH 3/7] feat!: handle multiple treatments & controls in samplesheet check --- assets/samplesheet_test_biowulf.csv | 6 +++--- assets/samplesheet_test_mle.csv | 10 +++++----- bin/check_samplesheet.py | 28 ++++++++++++++++------------ main.nf | 3 +++ subworkflows/local/input_check.nf | 4 +++- 5 files changed, 30 insertions(+), 21 deletions(-) diff --git a/assets/samplesheet_test_biowulf.csv b/assets/samplesheet_test_biowulf.csv index 2f7f3e4..aec877e 100644 --- a/assets/samplesheet_test_biowulf.csv +++ b/assets/samplesheet_test_biowulf.csv @@ -1,3 +1,3 @@ -sample,fastq_1,fastq_2,treat_or_ctrl -plasmid,/data/CCBR_Pipeliner/testdata/CRISPIN/ERR376998.fastq.gz,,control -ESC1,/data/CCBR_Pipeliner/testdata/CRISPIN/ERR376999.fastq.gz,,treatment +sample,rep,fastq_1,fastq_2,control +plasmid,,/data/CCBR_Pipeliner/testdata/CRISPIN/ERR376998.fastq.gz,, +ESC1,,/data/CCBR_Pipeliner/testdata/CRISPIN/ERR376999.fastq.gz,,plasmid diff --git a/assets/samplesheet_test_mle.csv b/assets/samplesheet_test_mle.csv index 6ebb874..be43aae 100644 --- a/assets/samplesheet_test_mle.csv +++ b/assets/samplesheet_test_mle.csv @@ -1,5 +1,5 @@ -sample,fastq_1,fastq_2,treat_or_ctrl -HL60.initial,tests/data/file1.fastq.gz,,control -KBM7.initial,tests/data/file1.fastq.gz,,control -HL60.final,tests/data/file2.fastq.gz,,treatment -KBM7.final,tests/data/file2.fastq.gz,,treatment +sample,rep,fastq_1,fastq_2,control +HL60.initial,,tests/data/file1.fastq.gz,,HL60.final +KBM7.initial,,tests/data/file1.fastq.gz,,KBM7.final +HL60.final,,tests/data/file2.fastq.gz,,treatment +KBM7.final,,tests/data/file2.fastq.gz,,treatment diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 27cb253..6d6baf1 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -8,6 +8,7 @@ import sys import errno import argparse +import pprint def parse_args(args=None): @@ -51,7 +52,7 @@ def check_samplesheet(file_in, file_out): with open(file_in, "r", encoding="utf-8-sig") as fin: ## Check header MIN_COLS = 2 - HEADER = ["sample", "fastq_1", "fastq_2", "treat_or_ctrl"] + HEADER = ["sample", "rep", "fastq_1", "fastq_2", "control"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print( @@ -81,7 +82,10 @@ def check_samplesheet(file_in, file_out): ) ## Check sample name entries - sample, fastq_1, fastq_2, treat_or_ctrl = lspl[: len(HEADER)] + sample_basename, rep, fastq_1, fastq_2, control = lspl[: len(HEADER)] + print("lspl") + pprint.pprint(lspl) + sample = f"{sample_basename}_{rep}" if rep else sample_basename if sample.find(" ") != -1: print( f"WARNING: Spaces have been replaced by underscores for sample: {sample}" @@ -102,14 +106,6 @@ def check_samplesheet(file_in, file_out): line, ) - ## check treatment or control designation - if treat_or_ctrl not in {"treatment", "control"}: - print_error( - "treat_or_ctrl column can only contain values `treatment` or `control`.", - "Line", - line, - ) - ## Auto-detect paired-end/single-end if sample and fastq_1 and fastq_2: ## Paired-end short reads is_single = "0" @@ -118,7 +114,7 @@ def check_samplesheet(file_in, file_out): else: print_error("Invalid combination of columns provided!", "Line", line) - sample_info = [is_single, fastq_1, fastq_2, treat_or_ctrl] + sample_info = [sample_basename, rep, is_single, fastq_1, fastq_2, control] ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2,]]} if sample not in sample_mapping_dict: sample_mapping_dict[sample] = [sample_info] @@ -135,7 +131,15 @@ def check_samplesheet(file_in, file_out): with open(file_out, "w") as fout: fout.write( ",".join( - ["sample", "single_end", "fastq_1", "fastq_2", "treat_or_ctrl"] + [ + "sample", + "sample_basename", + "rep", + "single_end", + "fastq_1", + "fastq_2", + "control", + ] ) + "\n" ) diff --git a/main.nf b/main.nf index b2d4d30..0f7e7fe 100644 --- a/main.nf +++ b/main.nf @@ -47,6 +47,7 @@ workflow { ch_count = TRIM_COUNT.out.count } + raw_reads .branch { meta, fastq -> treat: meta.treat_or_ctrl == 'treatment' @@ -58,6 +59,7 @@ workflow { treat = treat_meta.treat.collect() control = treat_meta.ctrl.collect() + if (params.mageck.run) { MAGECK(ch_count, treat, control) } @@ -67,4 +69,5 @@ workflow { if (params.bagel.run) { BAGEL(ch_count, control) } + } diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 7abe29d..cc96058 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -25,8 +25,10 @@ workflow INPUT_CHECK { def create_fastq_channel(LinkedHashMap row) { def meta = [:] meta.id = row.sample + meta.sample_basename = row.sample_basename + meta.rep = row.rep meta.single_end = row.single_end.toBoolean() - meta.treat_or_ctrl = row.treat_or_ctrl + meta.control = row.control // add path(s) of the fastq file(s) to the meta map def fastq_meta = [] From 944de4f7ad6dedb5e4b9f4ded12877fbffa483b5 Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 17:37:38 -0400 Subject: [PATCH 4/7] fix: reduce stringency of fastq extension --- bin/check_samplesheet.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 6d6baf1..8afa98f 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -99,9 +99,9 @@ def check_samplesheet(file_in, file_out): if fastq: if fastq.find(" ") != -1: print_error("FastQ file contains spaces!", "Line", line) - if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + if not fastq.endswith("fastq.gz") and not fastq.endswith(".fq.gz"): print_error( - "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "FastQ file does not have extension 'fastq.gz' or '.fq.gz'!", "Line", line, ) From 57d21efe68e66f4f28127c67aece7c7559b09fc5 Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 17:38:01 -0400 Subject: [PATCH 5/7] chore: note that onComplete should create log dir if DNE --- lib/Utils.groovy | 1 + 1 file changed, 1 insertion(+) diff --git a/lib/Utils.groovy b/lib/Utils.groovy index 3fe6464..0ea4ad2 100644 --- a/lib/Utils.groovy +++ b/lib/Utils.groovy @@ -15,6 +15,7 @@ class Utils { } catch(IOException e) { err = e } + // TODO create log/ dir if it doesn't exist new FileWriter("${workflow.launchDir}/log/spooker.log").with { write("${out}\n${err}") flush() From f176e55524aef5d5183c7e9540a937466e398d8b Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Thu, 23 May 2024 17:38:46 -0400 Subject: [PATCH 6/7] feat: [WIP] combine treatments & controls into one channel --- main.nf | 18 ++++++++++++++++-- 1 file changed, 16 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 0f7e7fe..5f5cc27 100644 --- a/main.nf +++ b/main.nf @@ -41,13 +41,27 @@ workflow { .reads .set { raw_reads } + ch_count = params.count_table ? file(params.count_table, checkIfExists: true) : null if (!ch_count) { // trim reads and run mageck count TRIM_COUNT(raw_reads, file(params.library, checkIfExists: true)) ch_count = TRIM_COUNT.out.count } - + ch_branched = raw_reads.branch { meta, fastqs -> + treat: meta.control.length() > 0 + return [ [control: meta.control], meta.id ] + control: meta.control.length() == 0 + return [ [control: meta.sample_basename] ] + } + ch_treat_ctrl_linked = + ch_branched.treat + | groupTuple() + | map { ctrl, ids -> [ ctrl, ids.flatten() ] } + | cross( ch_branched.control ) + //raw_reads | view + ch_treat_ctrl_linked | view +/* raw_reads .branch { meta, fastq -> treat: meta.treat_or_ctrl == 'treatment' @@ -69,5 +83,5 @@ workflow { if (params.bagel.run) { BAGEL(ch_count, control) } - +*/ } From 79d74b25ef3cd7370e15b30b15627cc81cb3124e Mon Sep 17 00:00:00 2001 From: Kelly Sovacool Date: Tue, 28 May 2024 17:20:02 -0400 Subject: [PATCH 7/7] fix: run processes after mageck count once per control --- .gitignore | 1 + assets/dag.png | Bin 123630 -> 127159 bytes assets/samplesheet_test_mle.csv | 8 +- conf/test.config | 4 +- conf/test_mle.config | 4 +- main.nf | 93 +++++++++------- modules/local/bagel.nf | 79 -------------- modules/local/bagel/bayes_factor/main.nf | 27 +++++ modules/local/bagel/fold_change/main.nf | 29 +++++ modules/local/bagel/precision_recall/main.nf | 27 +++++ modules/local/{drugz.nf => drugz/main.nf} | 12 +- modules/local/mageck.nf | 109 ------------------- modules/local/mageck/count/main.nf | 35 ++++++ modules/local/mageck/mle/main.nf | 32 ++++++ modules/local/mageck/test/main.nf | 30 +++++ modules/local/mageck/vispr/main.nf | 15 +++ subworkflows/local/bagel.nf | 23 ++-- subworkflows/local/mageck.nf | 20 ++-- subworkflows/local/trim_count.nf | 26 ----- 19 files changed, 285 insertions(+), 289 deletions(-) delete mode 100644 modules/local/bagel.nf create mode 100644 modules/local/bagel/bayes_factor/main.nf create mode 100644 modules/local/bagel/fold_change/main.nf create mode 100644 modules/local/bagel/precision_recall/main.nf rename modules/local/{drugz.nf => drugz/main.nf} (69%) delete mode 100644 modules/local/mageck.nf create mode 100644 modules/local/mageck/count/main.nf create mode 100644 modules/local/mageck/mle/main.nf create mode 100644 modules/local/mageck/test/main.nf create mode 100644 modules/local/mageck/vispr/main.nf delete mode 100644 subworkflows/local/trim_count.nf diff --git a/.gitignore b/.gitignore index 097fd6b..3ed297a 100644 --- a/.gitignore +++ b/.gitignore @@ -28,6 +28,7 @@ replay_pid* work/ data/ results/ +output/ /params.yaml # python 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zm82GLrY^R&Dz7Zv6YTW6~D$kr&%N{$1Wd zz%5Skf10TsxRIJ!@c8l6U+;K?zG!#>ePS%SVWJ0iZhUOQJzn= zyR*m&Tkyk2js!Ja_aR=_AP;OYnC0Lzk&AxUOIf*~q2coG5q94zZWR~*#hjzwE5&6L z@8IrZW@1&7^hFP7tk8omC# tA8Q}A;ZvmYXAKj?-|xEqmsR?s&DvS>0)HF7ZLj$8l!=yRu@fA({Wlc&NXq~K diff --git a/assets/samplesheet_test_mle.csv b/assets/samplesheet_test_mle.csv index be43aae..3074509 100644 --- a/assets/samplesheet_test_mle.csv +++ b/assets/samplesheet_test_mle.csv @@ -1,5 +1,5 @@ sample,rep,fastq_1,fastq_2,control -HL60.initial,,tests/data/file1.fastq.gz,,HL60.final -KBM7.initial,,tests/data/file1.fastq.gz,,KBM7.final -HL60.final,,tests/data/file2.fastq.gz,,treatment -KBM7.final,,tests/data/file2.fastq.gz,,treatment +HL60.initial,,tests/data/file1.fastq.gz,, +KBM7.initial,,tests/data/file1.fastq.gz,, +HL60.final,,tests/data/file2.fastq.gz,,HL60.initial +KBM7.final,,tests/data/file2.fastq.gz,,KBM7.initial diff --git a/conf/test.config b/conf/test.config index 43ed959..e06b040 100644 --- a/conf/test.config +++ b/conf/test.config @@ -4,10 +4,10 @@ params { input = 'assets/samplesheet_test_biowulf.csv' library = 'assets/lib/yusa_library.csv' - outdir = 'results/test' + outdir = 'output/test' exp_name = 'test' - publish_dir_mode = 'symlink' + publish_dir_mode = 'link' mageck.run = true diff --git a/conf/test_mle.config b/conf/test_mle.config index 687e226..c79b58d 100644 --- a/conf/test_mle.config +++ b/conf/test_mle.config @@ -4,9 +4,11 @@ params { input = 'assets/samplesheet_test_mle.csv' library = 'assets/lib/yusa_library.csv' - outdir = 'results/test_mle' + outdir = 'output/test_mle' exp_name = 'leukemia' + publish_dir_mode = 'link' + count_table = '/data/CCBR_Pipeliner/testdata/CRISPIN/leukemia.new.csv' design_matrix = '/data/CCBR_Pipeliner/testdata/CRISPIN/designmat.txt' diff --git a/main.nf b/main.nf index 5f5cc27..f7f2e03 100644 --- a/main.nf +++ b/main.nf @@ -18,12 +18,13 @@ input : ${params.input} // SUBMODULES include { INPUT_CHECK } from './subworkflows/local/input_check.nf' -include { TRIM_COUNT } from './subworkflows/local/trim_count.nf' include { MAGECK } from './subworkflows/local/mageck.nf' include { BAGEL } from './subworkflows/local/bagel.nf' // MODULES -include { DRUGZ } from './modules/local/drugz.nf' +include { CUTADAPT } from './modules/CCBR/cutadapt' +include { MAGECK_COUNT } from './modules/local/mageck/count' +include { DRUGZ } from './modules/local/drugz' workflow.onComplete { if (!workflow.stubRun && !workflow.commandLine.contains('-preview')) { @@ -37,51 +38,67 @@ workflow.onComplete { workflow { INPUT_CHECK(file(params.input)) - INPUT_CHECK.out - .reads - .set { raw_reads } + INPUT_CHECK.out.reads + | set { raw_reads } + if (params.count_table) { - ch_count = params.count_table ? file(params.count_table, checkIfExists: true) : null - if (!ch_count) { // trim reads and run mageck count - TRIM_COUNT(raw_reads, file(params.library, checkIfExists: true)) - ch_count = TRIM_COUNT.out.count - } + // if count table exists, re-populate metadata with linked controls + ch_branched = raw_reads.branch { meta, fastqs -> + treat: meta.control.length() > 0 + return [ [control: meta.control], meta.id ] + control: meta.control.length() == 0 + return [ [control: meta.sample_basename], meta.id ] + } + ch_count = ch_branched.treat + | groupTuple() + | map { meta, ids -> + [ meta, ids.flatten().join(',')] + } + | join( ch_branched.control ) + | map { meta, ids, ctrl -> + meta.ids = ids + [ meta ] + } + | combine( Channel.fromPath(file(params.count_table, checkIfExists: true)) ) + ch_count | view + } else { + // trim reads and run mageck count + CUTADAPT(raw_reads) + + // group samples with their controls + ch_branched = CUTADAPT.out.reads.branch { meta, fastqs -> + treat: meta.control.length() > 0 + return [ [control: meta.control], meta.id, fastqs ] + control: meta.control.length() == 0 + return [ [control: meta.sample_basename], fastqs ] + } + ch_treat_ctrl_linked = ch_branched.treat + | groupTuple() + | map { meta, ids, fastqs -> + [ meta, ids.flatten().join(','), fastqs.flatten() ] + } + | join( ch_branched.control ) + | map { meta, ids, sample_fastqs, ctrl_fastqs -> + meta.ids = ids + [ meta, sample_fastqs, ctrl_fastqs ] + } - ch_branched = raw_reads.branch { meta, fastqs -> - treat: meta.control.length() > 0 - return [ [control: meta.control], meta.id ] - control: meta.control.length() == 0 - return [ [control: meta.sample_basename] ] - } - ch_treat_ctrl_linked = - ch_branched.treat - | groupTuple() - | map { ctrl, ids -> [ ctrl, ids.flatten() ] } - | cross( ch_branched.control ) - //raw_reads | view - ch_treat_ctrl_linked | view -/* - raw_reads - .branch { meta, fastq -> - treat: meta.treat_or_ctrl == 'treatment' - return meta.id - ctrl: meta.treat_or_ctrl == 'control' - return meta.id - } - .set { treat_meta } + MAGECK_COUNT(ch_treat_ctrl_linked, + file(params.library, checkIfExists: true) + ) + ch_count = MAGECK_COUNT.out.count + } - treat = treat_meta.treat.collect() - control = treat_meta.ctrl.collect() if (params.mageck.run) { - MAGECK(ch_count, treat, control) + MAGECK(ch_count) } if (params.drugz.run) { - DRUGZ(ch_count, treat, control) + DRUGZ(ch_count) } if (params.bagel.run) { - BAGEL(ch_count, control) + BAGEL(ch_count) } -*/ + } diff --git a/modules/local/bagel.nf b/modules/local/bagel.nf deleted file mode 100644 index 553b014..0000000 --- a/modules/local/bagel.nf +++ /dev/null @@ -1,79 +0,0 @@ - -process FOLD_CHANGE { - label 'bagel' - container "${params.containers.bagel}" - - input: - path(count) - val(control) - - output: - path("*.foldchange"), emit: fc - path("*.normed_readcount"), emit: count_norm - - script: - """ - BAGEL.py fc \\ - -i ${count} \\ - -o ${count.getBaseName(2)} \\ - -c ${control.join(',')} - """ - - stub: - """ - for ext in foldchange normed_readcount; do - touch ${count.getBaseName(2)}.\$ext - done - echo ${task.container} > output.txt - """ -} - -process BAYES_FACTOR { - label 'bagel' - container "${params.containers.bagel}" - - input: - path(fold_change) - - output: - path("*.bf"), emit: bf - - script: - """ - BAGEL.py bf \\ - -i ${fold_change} \\ - -o ${fold_change.getBaseName(2)}.bf \\ - -e ${params.bagel.core_essential_genes} \\ - -n ${params.bagel.non_essential_genes} \\ - -c ${params.bagel.test_columns} - """ - - stub: - """ - touch ${fold_change.getBaseName(2)}.bf - """ -} -process PRECISION_RECALL { - label 'bagel' - container "${params.containers.bagel}" - - input: - path(bayes_factor) - - output: - path("*.pr"), emit: pr - - script: - """ - BAGEL.py pr \\ - -i ${bayes_factor} \\ - -o ${bayes_factor.getBaseName(2)}.pr \\ - -e ${params.bagel.core_essential_genes} \\ - -n ${params.bagel.non_essential_genes} - """ - - stub: - """ - touch ${bayes_factor.getBaseName(2)}.pr - """ -} diff --git a/modules/local/bagel/bayes_factor/main.nf b/modules/local/bagel/bayes_factor/main.nf new file mode 100644 index 0000000..9930252 --- /dev/null +++ b/modules/local/bagel/bayes_factor/main.nf @@ -0,0 +1,27 @@ + +process BAGEL_BAYES_FACTOR { + tag { meta.control } + container "${params.containers.bagel}" + label 'process_single' + + input: + tuple val(meta), path(fold_change) + + output: + tuple val(meta), path("*.bf"), emit: bf + + script: + """ + BAGEL.py bf \\ + -i ${fold_change} \\ + -o ${fold_change.getBaseName(2)}.bf \\ + -e ${params.bagel.core_essential_genes} \\ + -n ${params.bagel.non_essential_genes} \\ + -c ${params.bagel.test_columns} + """ + + stub: + """ + touch ${fold_change.getBaseName(2)}.bf + """ +} diff --git a/modules/local/bagel/fold_change/main.nf b/modules/local/bagel/fold_change/main.nf new file mode 100644 index 0000000..a7d5478 --- /dev/null +++ b/modules/local/bagel/fold_change/main.nf @@ -0,0 +1,29 @@ + +process BAGEL_FOLD_CHANGE { + tag { meta.control } + container "${params.containers.bagel}" + label 'process_single' + + input: + tuple val(meta), path(count) + + output: + tuple val(meta), path("*.foldchange"), emit: fc + tuple val(meta), path("*.normed_readcount"), emit: count_norm + + script: + """ + BAGEL.py fc \\ + -i ${count} \\ + -o ${count.getBaseName(2)} \\ + -c ${meta.control} + """ + + stub: + """ + for ext in foldchange normed_readcount; do + touch ${count.getBaseName(2)}.\$ext + done + echo ${task.container} > output.txt + """ +} diff --git a/modules/local/bagel/precision_recall/main.nf b/modules/local/bagel/precision_recall/main.nf new file mode 100644 index 0000000..279b276 --- /dev/null +++ b/modules/local/bagel/precision_recall/main.nf @@ -0,0 +1,27 @@ + + +process BAGEL_PRECISION_RECALL { + tag { meta.control } + container "${params.containers.bagel}" + label 'process_single' + + input: + tuple val(meta), path(bayes_factor) + + output: + tuple val(meta), path("*.pr"), emit: pr + + script: + """ + BAGEL.py pr \\ + -i ${bayes_factor} \\ + -o ${bayes_factor.getBaseName(2)}.pr \\ + -e ${params.bagel.core_essential_genes} \\ + -n ${params.bagel.non_essential_genes} + """ + + stub: + """ + touch ${bayes_factor.getBaseName(2)}.pr + """ +} diff --git a/modules/local/drugz.nf b/modules/local/drugz/main.nf similarity index 69% rename from modules/local/drugz.nf rename to modules/local/drugz/main.nf index fcc2d62..4109f1e 100644 --- a/modules/local/drugz.nf +++ b/modules/local/drugz/main.nf @@ -4,13 +4,11 @@ process DRUGZ { container "${params.containers.drugz}" input: - path(count) - val(treatment) - val(control) + tuple val(meta), path(count) output: - path("*output.txt"), emit: txt - path("*foldchange.txt"), emit: fc + tuple val(meta), path("*output.txt"), emit: txt + tuple val(meta), path("*foldchange.txt"), emit: fc script: """ @@ -18,8 +16,8 @@ process DRUGZ { -i ${count} \\ -o ${count.getBaseName(2)}.output.txt \\ -f ${count.getBaseName(2)}.foldchange.txt \\ - -c ${control.join(',')} \\ - -x ${treatment.join(',')} \\ + -c ${meta.control} \\ + -x ${meta.ids} \\ -r ${params.drugz.remove_genes} \\ --half_window_size ${params.drugz.half_window_size} """ diff --git a/modules/local/mageck.nf b/modules/local/mageck.nf deleted file mode 100644 index a838a26..0000000 --- a/modules/local/mageck.nf +++ /dev/null @@ -1,109 +0,0 @@ - -process COUNT { - label 'mageck' - container "${params.containers.mageck}" - - input: - file(lib) - val(ids) - path(fastqs) - - output: - path("*.count.txt"), emit: count - path("*.count_normalized.txt"), emit: count_norm - path("*.countsummary.txt"), emit: count_sum - - script: - """ - mageck count \\ - -l ${lib} \\ - --fastq ${fastqs} \\ - --sample-label ${ids.join(',')} \\ - -n ${params.exp_name} - """ - - stub: - """ - for ext in count count_normalized countsummary; do - touch ${params.exp_name}.\${ext}.txt - done - uname -a > output.txt - echo ${task.container} >> output.txt - """ - -} - -process TEST { - label 'mageck' - container "${params.containers.mageck}" - - input: - path(count) - val(treatment) - val(control) - - output: - path("*.gene_summary.txt"), emit: gene - path("*.sgrna_summary.txt"), emit: sgrna - - script: - """ - mageck test \\ - -k ${count} \\ - -t ${treatment.join(',')} \\ - -c ${control.join(',')} \\ - -n ${count.getBaseName(2)} - """ - - stub: - """ - for ext in gene sgrna; do - touch ${count.getBaseName(2)}.\${ext}_summary.txt - done - """ -} - -process MLE { - label 'mageck' - label 'process_higher' - container "${params.containers.mageck}" - - input: - path(count) - path(design) - - output: - path("*.gene_summary.txt"), emit: gene - path("*.sgrna_summary.txt"), emit: sgrna - - script: - """ - export OMP_NUM_THREADS=1 # this number gets multiplied by --threads - mageck mle \\ - -k ${count} \\ - -d ${design} \\ - -n ${count.getBaseName(2)} \\ - --threads ${task.cpus} - """ - - stub: - """ - for ext in gene sgrna; do - touch ${count.getBaseName(2)}.\${ext}_summary.txt - done - """ -} - -process VISPR { // TODO - label 'vispr' - container "${params.containers.vispr}" - - output: - path("output.txt") - - script: - """ - uname -a >> output.txt - which vispr >> output.txt - """ -} diff --git a/modules/local/mageck/count/main.nf b/modules/local/mageck/count/main.nf new file mode 100644 index 0000000..1fb3cdd --- /dev/null +++ b/modules/local/mageck/count/main.nf @@ -0,0 +1,35 @@ + +process MAGECK_COUNT { + + tag { meta.control } + container "${params.containers.mageck}" + label 'process_single' + + input: + tuple val(meta), path(fastqs_sample), path(fastqs_control) + path(lib) + + output: + tuple val(meta), path("*.count.txt"), emit: count + tuple val(meta), path("*.count_normalized.txt"), emit: count_norm + tuple val(meta), path("*.countsummary.txt"), emit: count_sum + + script: + """ + mageck count \\ + -l ${lib} \\ + --fastq ${fastqs_sample} ${fastqs_control} \\ + --sample-label ${meta.ids},${meta.control} \\ + -n ${params.exp_name} + """ + + stub: + """ + for ext in count count_normalized countsummary; do + touch ${params.exp_name}.\${ext}.txt + done + uname -a > output.txt + echo ${task.container} >> output.txt + """ + +} diff --git a/modules/local/mageck/mle/main.nf b/modules/local/mageck/mle/main.nf new file mode 100644 index 0000000..35b43c4 --- /dev/null +++ b/modules/local/mageck/mle/main.nf @@ -0,0 +1,32 @@ + + +process MAGECK_MLE { + tag { meta.control } + label 'process_higher' + container "${params.containers.mageck}" + + input: + tuple val(meta), path(count) + path(design) + + output: + tuple val(meta), path("*.gene_summary.txt"), emit: gene + tuple val(meta), path("*.sgrna_summary.txt"), emit: sgrna + + script: + """ + export OMP_NUM_THREADS=1 # this number gets multiplied by --threads + mageck mle \\ + -k ${count} \\ + -d ${design} \\ + -n ${count.getBaseName(2)} \\ + --threads ${task.cpus} + """ + + stub: + """ + for ext in gene sgrna; do + touch ${count.getBaseName(2)}.\${ext}_summary.txt + done + """ +} diff --git a/modules/local/mageck/test/main.nf b/modules/local/mageck/test/main.nf new file mode 100644 index 0000000..50d61ed --- /dev/null +++ b/modules/local/mageck/test/main.nf @@ -0,0 +1,30 @@ + +process MAGECK_TEST { + + tag { meta.control } + container "${params.containers.mageck}" + label 'process_single' + + input: + tuple val(meta), path(count) + + output: + tuple val(meta), path("*.gene_summary.txt"), emit: gene + tuple val(meta), path("*.sgrna_summary.txt"), emit: sgrna + + script: + """ + mageck test \\ + -k ${count} \\ + -t ${meta.ids} \\ + -c ${meta.control} \\ + -n ${count.getBaseName(2)} + """ + + stub: + """ + for ext in gene sgrna; do + touch ${count.getBaseName(2)}.\${ext}_summary.txt + done + """ +} diff --git a/modules/local/mageck/vispr/main.nf b/modules/local/mageck/vispr/main.nf new file mode 100644 index 0000000..a621f76 --- /dev/null +++ b/modules/local/mageck/vispr/main.nf @@ -0,0 +1,15 @@ + + +process MAGECK_VISPR { // TODO + label 'vispr' + container "${params.containers.vispr}" + + output: + path("output.txt") + + script: + """ + uname -a >> output.txt + which vispr >> output.txt + """ +} diff --git a/subworkflows/local/bagel.nf b/subworkflows/local/bagel.nf index 1b0ab04..e2b9589 100644 --- a/subworkflows/local/bagel.nf +++ b/subworkflows/local/bagel.nf @@ -1,20 +1,19 @@ -include { FOLD_CHANGE } from '../../modules/local/bagel.nf' -include { BAYES_FACTOR } from '../../modules/local/bagel.nf' -include { PRECISION_RECALL } from '../../modules/local/bagel.nf' +include { BAGEL_FOLD_CHANGE } from '../../modules/local/bagel/fold_change' +include { BAGEL_BAYES_FACTOR } from '../../modules/local/bagel/bayes_factor' +include { BAGEL_PRECISION_RECALL } from '../../modules/local/bagel/precision_recall' workflow BAGEL { take: - count - ctrl + ch_count main: - FOLD_CHANGE(count, ctrl) - BAYES_FACTOR(FOLD_CHANGE.out.fc) - PRECISION_RECALL(BAYES_FACTOR.out.bf) + BAGEL_FOLD_CHANGE(ch_count) + BAGEL_BAYES_FACTOR(BAGEL_FOLD_CHANGE.out.fc) + BAGEL_PRECISION_RECALL(BAGEL_BAYES_FACTOR.out.bf) emit: - fold_change = FOLD_CHANGE.out.fc - count_norm = FOLD_CHANGE.out.count_norm - bayes_factor = BAYES_FACTOR.out.bf - prec_rec = PRECISION_RECALL.out.pr + fold_change = BAGEL_FOLD_CHANGE.out.fc + count_norm = BAGEL_FOLD_CHANGE.out.count_norm + bayes_factor = BAGEL_BAYES_FACTOR.out.bf + prec_rec = BAGEL_PRECISION_RECALL.out.pr } diff --git a/subworkflows/local/mageck.nf b/subworkflows/local/mageck.nf index 9483206..15b1808 100644 --- a/subworkflows/local/mageck.nf +++ b/subworkflows/local/mageck.nf @@ -1,28 +1,26 @@ -include { TEST } from "../../modules/local/mageck.nf" -include { MLE } from "../../modules/local/mageck.nf" +include { MAGECK_TEST } from "../../modules/local/mageck/test" +include { MAGECK_MLE } from "../../modules/local/mageck/mle" workflow MAGECK { take: - count - treat - ctrl + ch_count main: - TEST(count, treat, ctrl) + MAGECK_TEST(ch_count) if (params.design_matrix) { - MLE(count, file(params.design_matrix, checkIfExists: true)) - mle_gene = MLE.out.gene - mle_sgrna = MLE.out.sgrna + MAGECK_MLE(ch_count, file(params.design_matrix, checkIfExists: true)) + mle_gene = MAGECK_MLE.out.gene + mle_sgrna = MAGECK_MLE.out.sgrna } else { mle_gene = null mle_sgrna = null } emit: - test_gene = TEST.out.gene - test_sgrna = TEST.out.sgrna + test_gene = MAGECK_TEST.out.gene + test_sgrna = MAGECK_TEST.out.sgrna mle_gene = mle_gene mle_sgrna = mle_sgrna diff --git a/subworkflows/local/trim_count.nf b/subworkflows/local/trim_count.nf deleted file mode 100644 index ef12f53..0000000 --- a/subworkflows/local/trim_count.nf +++ /dev/null @@ -1,26 +0,0 @@ -include { CUTADAPT } from '../../modules/CCBR/cutadapt' -include { COUNT as MAGECK_COUNT } from "../../modules/local/mageck.nf" - -workflow TRIM_COUNT { - take: - raw_reads - library - - main: - CUTADAPT(raw_reads) - CUTADAPT.out.reads - .multiMap { meta, fastq -> - id: meta.id - fastq: fastq - } - .set{ reads } - - MAGECK_COUNT(library, - reads.id.collect(), - reads.fastq.collect() - ) - - emit: - count = MAGECK_COUNT.out.count - trimmed_reads = CUTADAPT.out.reads -}