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mrfast does not recognize the '--threads ' flag #9

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johnemajor opened this issue Dec 7, 2019 · 1 comment
Open

mrfast does not recognize the '--threads ' flag #9

johnemajor opened this issue Dec 7, 2019 · 1 comment

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@johnemajor
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johnemajor commented Dec 7, 2019

I'm running 1.0.6 version of Tardis. I've installed mrfastat version 'mrFAST 2.6.1.0 with FastHASH'. When I run tardis with --sensitive and --threads 96, I get the following error from mrfast:

logs/SENSITIVE.tardis.log:/home/ubuntu/conda/envs/wgspy3/bin/mrfast: unrecognized option '--threads'

@johnemajor
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johnemajor commented Dec 7, 2019

Here are all of the valid mrfast command line options:

`mrFAST : Micro-Read Fast Alignment Search Tool. Enhanced with FastHASH.

Usage: mrfast [options]

General Options:
-v|--version Current Version.
-h Shows the help file.

Indexing Options:
--index [file] Generate an index from the specified fasta file.
--ws [int] Set window size for indexing (default:12 max:14).

Searching Options:
--search [file] Search in the specified genome. Provide the path to the fasta file.
Index file should be in the same directory.
--pe Search will be done in Paired-End mode.
--seq [file] Input sequences in fasta/fastq format [file]. If
paired end reads are interleaved, use this option.
--seq1 [file] Input sequences in fasta/fastq format [file] (First
file). Use this option to indicate the first file of
paired end reads.
--seq2 [file] Input sequences in fasta/fastq format [file] (Second
file). Use this option to indicate the second file of
paired end reads.
-o [file] Output of the mapped sequences. The default is "output".
-u [file] Save unmapped sequences in fasta/fastq format.
--best Only the best mapping from all the possible mapping is returned.
--seqcomp Indicates that the input sequences are compressed (gz).
--outcomp Indicates that output file should be compressed (gz).
-e [int] Maximum allowed edit distance (default 4% of the read length).
--min [int] Min distance allowed between a pair of end sequences.
--max [int] Max distance allowed between a pair of end sequences.
--maxoea [int] Max number of One End Anchored (OEA) returned for each read pair.
We recommend 100 or above for NovelSeq use. Default = 100.
--maxdis [int] Max number of discordant map locations returned for each read pair.
We recommend 300 or above for VariationHunter use. Default = 300.
--crop [int] Trim the reads to the given length.
--sample [string] Sample name to be added to the SAM header (optional).
--rg [string] Read group ID to be added to the SAM header (optional).
--lib [string] Library name to be added to the SAM header (optional).

`

@johnemajor johnemajor changed the title mrfast does not recognize the ' mrfast does not recognize the '--threads ' flag Dec 8, 2019
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