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nextflow.config
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nextflow.config
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manifest {
author = 'Dan Fornika'
name = 'BCCDC-PHL/routine-assembly'
version = '0.4.5'
description = 'BCCDC-PHL Routine Assembly'
mainScript = 'main.nf'
nextflowVersion = '>=20.01.0'
}
params {
prokka = false
bakta = false
hybrid = false
long_only = false
bakta_db = '/data/ref_databases/bakta/latest'
illumina_suffixes = ['*_R{1,2}_001', '*_R{1,2}', '*_{1,2}' ]
long_read_suffixes = ['*_RL', '*_L']
fastq_exts = ['.fastq.gz', '.fq.gz', '.fastq', '.fq']
fastq_search_path = makeFastqSearchPath(illumina_suffixes, fastq_exts)
fastq_input = 'NO_FILE'
long_reads_search_path = makeLongReadsSearchPath(long_read_suffixes, fastq_exts)
fastq_input_long = 'NO_FILE'
filtlong_min_length = 1000
filtlong_keep_percent = 90
samplesheet_input = 'NO_FILE'
use_sample_id_as_annotation_locustag = false
annotation_centre = 'BCCDC-PHL'
pipeline_short_name = parsePipelineName(manifest.toMap().get('name'))
pipeline_minor_version = parseMinorVersion(manifest.toMap().get('version'))
}
def makeFastqSearchPath(illumina_suffixes, fastq_exts) {
def fastq_search_path = []
if (params.fastq_input.toString() != 'NO_FILE') {
for (suffix in illumina_suffixes) {
for (ext in fastq_exts) {
fastq_search_path.add(params.fastq_input.toString() + '/' + suffix.toString() + ext.toString())
}
}
}
return fastq_search_path
}
def makeLongReadsSearchPath(long_read_suffixes, fastq_exts) {
def fastq_search_path = []
if (params.fastq_input_long.toString() != 'NO_FILE') {
for (suffix in long_read_suffixes) {
for (ext in fastq_exts) {
fastq_search_path.add(params.fastq_input_long.toString() + '/' + suffix.toString() + ext.toString())
}
}
}
return fastq_search_path
}
def parseMinorVersion(version) {
minor_version = version.split('\\.')[0..1].join('.')
return minor_version
}
def parsePipelineName(name) {
short_name = name.split('/')[1]
return short_name
}
profiles {
conda {
conda.enabled = true
process.conda = "$baseDir/environments/environment.yml"
if (params.cache){
conda.cacheDir = params.cache
}
}
}
// Capture exit codes from upstream processes when piping
process.shell = ['/bin/bash', '-euo', 'pipefail']
process {
withName: quast {
cpus = 2
}
withName: fastp {
cpus = 4
}
withName: filtlong {
cpus = 12
}
withName: unicycler {
cpus = 12
}
withName: prokka {
cpus = 8
}
withName: bakta {
cpus = 12
conda = "$baseDir/environments/bakta.yml"
}
}