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CRAM support #2

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xtmgah opened this issue Oct 9, 2020 · 6 comments
Open

CRAM support #2

xtmgah opened this issue Oct 9, 2020 · 6 comments

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@xtmgah
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xtmgah commented Oct 9, 2020

Hello

I want to know if AR supports CRAM file as input? Thanks.

@jluebeck
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jluebeck commented Oct 9, 2020

Hi,

AR does not directly take a BAM/CRAM as input - only the AA-generated breakpoint graph derived from those files. AA is compatible with CRAM input, as per the AA README.

The workflow for analysis using AR involves running AA first, then running AR using that output along with the Bionano data.

@ZHONGHONGXIN
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Hi,

AR does not directly take a BAM/CRAM as input - only the AA-generated breakpoint graph derived from those files. AA is compatible with CRAM input, as per the AA README.

The workflow for analysis using AR involves running AA first, then running AR using that output along with the Bionano data.

Could you please tell me something about the address? That l can find more cmap files, thanks. Now, l jump into the AR, but l can't find the cmap file.

@jluebeck
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Hi, cmap files are generated from Bionano optical mapping. This is an entirely separate assay from whole-genome sequencing. The NCBI keeps a repository of Bionano OM data here.

@ZHONGHONGXIN
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Hi, cmap files are generated from Bionano optical mapping. This is an entirely separate assay from whole-genome sequencing. The NCBI keeps a repository of Bionano OM data here.

Hi, jluebeck

l find the human lung camp file to replace the real sample camp file because l can find my sample cmap file; l get the eccDNA circle, but l don't know this result whether or not correct. Could you please tell me some advice, thanks?

HongXin

@jluebeck
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Hi HongXin,

Sure, you can email me results at jluebeck [a t] ucsd.edu and I can take a look.

Jens

@ZHONGHONGXIN
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Hi HongXin,

Sure, you can email me results at jluebeck [a t] ucsd.edu and I can take a look.

Jens

Thanks, l send an email to your adress just now.

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